Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Reverse (-)

30529544 .. 30536827

Gene Structure 7284 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG277300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGGCGGCTCCAGATTCCGGCCCCCAGGGGCTGACGCGACGGCCGTCACGGAGCGCCGCGACGACGACGTTTTCGACGGAGGTCTTCGACAACGAGGTCGTCCCCTCCGCCATCGCCCCTATCGCTCCGCTCCTCCGCGTCGCCAACGAAGTCGAGCCCGAGCGCCCTCGCGTCGCCTATCTCTGTCGGTTCTACGCGTTTGAGAAGGCGCACAGATTGGACCCCGGCTCCAGCGGCCGTGGTGTGAGGCAGTTCAAGACCTTGCTCCTGCAGCGATTGGAGAGGGAAAATGCATCAAGTCTTTCTGCTCGAGTTAAAAAGTCTGATGCCAGGGAAATTACAAGCTTCTATCAGCAATATTATGATCACTATGTTAAAGCTGTTCAAGAGGAACAGGTAGACAGAGCTCAAGTGAGTAAAGCCTACAATACACTTAACCAGCTTTTTGAAGTACTTTGCGGGGTTAACAAGTCAGAGAAAGTTGAAGAAGTTGCTCCTGAGATTATTGCAGCCGCTAGAGATGTCCAAGAGAAGACGGAAATATACGCCCCGTATAATATTCTTCCTTTGGATTCTGCTGGTGCTACACAGTCTGTCATGCAGCTTGACGAGGTTAAGGCCGCTGTGGCTGCACTGTGGAATACTCGTGGTTTAAACTGGCCAAGTGCATTCGAGCAGCACAGACAAAAAGCTAATGATTTGGATCTCCTTGATTGGCTTAGGGCCATGTTTGGGTTCCAGAGAGATAACGTCAGGAACCAGAGGGAGCATTTGATATTGCTACTTGCGAATACGCATATAAGACTTCATCCCAAACCTGAACCTCTTAACAAGCTTGATGATCGAGCTGTTGATGCAGTTCTGGACAAGCTTTTTAAGAACTACAAAACGTGGTGTAAATTTTTGGGTCGAAAACATAGTTTAAGGCTACCTCAAGGTCAGCAAGAAGTACAACAGAGGAAGATACTTTACATGGGTTTATATCTTCTCATATGGGGTGAAGCAGCGAATGTTCGCTTCATGCCTGAATGCCTCTGCTATATTTTCCATAATATGGCATATGAACTCCATGGTCTCTTGGCTGGAAATGTCAGCATTGTCACTGGGGAAAACATAAAGCCTTCTTATGGTGGGGATGATGAGGCCTTTTTGAGGAAGGTTATAACCCCCCTATACCGTGTAATCGAAAAGGAAGCCAAGAAGGGCGGCGGTGGAAAAGCTCCTCACACAGCTTGGTGCAACTATGATGATTTAAATGAATATTTCTGGTCATCTGATTGTTTCTCTCTTGGTTGGCCCATGCGTGATGATGGGGATTTTTTTAAATCAACTCGTGACTTGGCACAGGGAAGAAAGGGCTCTAAAAGAAAATCTGGAAGCACAGGCAAATCATATTTTATTGAAACTCGCACATTTTGGCATATCTTTCGAAGTTATGATCGAATGTGGACCTTTTATATACTGGCTCTACAGGCTATGCTCATTATTGCATTCAGCAAGGTTTCACCGCTGGATATATTTCAACAGGATGTCCTAAAAGATCTATCAAGTATTTTCATTACAGCAGCATTTCTTCGCGTCCTTCAGAGTATTCTGGATATGGTTTTGAACTTCCCTGGATATCACAGGTGTCGGTTCACAGATGTCCTGAGAAATATTCTGAAGATAGTTGTTAGTCTTGCATGGGCAGTCATTCTTCCACTTTTCTATGTGCATTCCTTCAATGATTCCCCTCAACAAATTGCAGATATACTGTCATTCCTTAAGAACATTCAAGGCGTTCCATCATTATATCTATTGGCTGTTGCAGTATATTTGCTTCCAAATTTACTGACAGCAGTTTTGTTCCTTTTCCCAATGCTCCGTCGTTTTATTGAAAATTCAGACTGGCATATCATCAGGTTCCTCTTGTGGTGGTCACAGCCAAGAATATATGTTGGAAGAGGAATGCATGAAAGTCAATTTGCCCTTCTAAAGTATACTTTTTTCTGGTTGCTACTTTTGGCTTGCAAGTTTACAGTTAGCTATTTTGTCCAGATAAAACCTCTGGTAGAGCCAACAGAAGACATAATGAAGATTCGTCGTATTAATTATGAGTGGCATGAATTTTTCCCTAATGCTGAACATAACTATGGAGCAGTTGTCTCACTCTGGGCACCTGTTATCATGGTTTATTTCATGGACACACAAATTTGGTATGCTATATTTCAAACATTATATGGAGGTATTGTTGGCGCCTTTGATCGCCTCGGAGAGATTCGTACTCTAGGCATGCTAAGATCCCGGTTCCAGTCTCTACCTGGTGCATTCAACACATATCTAGTGCCCTCGGATAAGTCAACAAAAAGGGGATTCTCTTTCTCAAAGAAATTTGCGGAGGCTTCAGCAAGTAGGAGAAGTGAAGCTGCAAAGTTTGCTCAGTTGTGGAATGAAGTGATATGTAGCTTTCGTGAGGAAGATCTCATCAGCGACAGGAAATGCTCTGCAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

830

Amino Acids

95.57

Weight (kDa)

9.06

Isoelectric Point (pI)

49.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vta1	PF04652	41 - 162	4.2e-13	Vta1 like
FKS1_dom1	PF14288	321 - 432	2.7e-41	1,3-beta-glucan synthase subunit FKS1, domain-1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1163
AasI	GACNNNNNNGTC	1 cut(s)	95
AccB1I	GGYRCC	2 cut(s)	2155, 2234
AccB7I	CCANNNNNTGG	1 cut(s)	1054
AccBSI	CCGCTC	1 cut(s)	130
AccI	GTMKAC	2 cut(s)	399, 1979
AccII	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
AclWI	GGATC	2 cut(s)	711, 2274
AcoI	YGGCCR	3 cut(s)	41, 235, 659
AcsI	RAATTY	6 cut(s)	899, 1825, 1879, 2105, 2190, 2369
AcuI	CTGAAG	3 cut(s)	1568, 1682, 2367
AcyI	GRCGYC	1 cut(s)	2235
AfaI	GTAC	3 cut(s)	453, 951, 2263
AfiI	CCNNNNNNNGG	5 cut(s)	17, 732, 1054, 1127, 1152
AflII	CTTAAG	1 cut(s)	1763
AflIII	ACRYGT	1 cut(s)	195
AjnI	CCWGG	4 cut(s)	24, 329, 1615, 2299
Alw21I	GWGCWC	1 cut(s)	409
Alw26I	GTCTC	3 cut(s)	1079, 2149, 2298
AlwI	GGATC	2 cut(s)	711, 2274
AlwNI	CAGNNNCTG	1 cut(s)	31
Ama87I	CYCGRG	2 cut(s)	158, 309
AoxI	GGCC	8 cut(s)	19, 41, 235, 618, 659, 723, 1143, 1295
ApeKI	GCWGC	8 cut(s)	271, 509, 601, 629, 676, 1004, 1565, 2405
ApoI	RAATTY	6 cut(s)	899, 1825, 1879, 2105, 2190, 2369
ArsI	GACNNNNNNTTYG	4 cut(s)	55, 87, 1825, 1857
AseI	ATTAAT	1 cut(s)	2088
AspLEI	GCGC	4 cut(s)	56, 165, 211, 2237
AspS9I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
AsuC2I	CCSGG	2 cut(s)	225, 2284
AsuHPI	GGTGA	2 cut(s)	1010, 1497
AsuII	TTCGAA	1 cut(s)	1429
AvaI	CYCGRG	2 cut(s)	158, 309
AvaII	GGWCC	2 cut(s)	220, 1449
BaeGI	GKGCMC	2 cut(s)	2158, 2328
BalI	TGGCCA	1 cut(s)	661
BanI	GGYRCC	2 cut(s)	2155, 2234
BanII	GRGCYC	3 cut(s)	159, 409, 1361
BarI	GAAGNNNNNNTAC	4 cut(s)	953, 985, 1803, 1835
BauI	CACGAG	2 cut(s)	645, 1332
BbsI	GAAGAC	3 cut(s)	76, 539, 2070
Bbv12I	GWGCWC	1 cut(s)	409
BbvI	GCAGC	8 cut(s)	283, 521, 613, 616, 688, 1016, 1577, 2392
BccI	CCATC	3 cut(s)	119, 1304, 1792
BceAI	ACGGC	3 cut(s)	28, 56, 222
BcgI	CGANNNNNNTGC	2 cut(s)	1409, 1443
BciT130I	CCWGG	4 cut(s)	26, 331, 1617, 2301
BclI	TGATCA	1 cut(s)	364
BcnI	CCSGG	2 cut(s)	225, 2284
BcoDI	GTCTC	3 cut(s)	1079, 2149, 2298
BfaI	CTAG	3 cut(s)	516, 2267, 2321
BfmI	CTRYAG	2 cut(s)	269, 1469
BfoI	RGCGCY	3 cut(s)	57, 166, 2238
BfrI	CTTAAG	1 cut(s)	1763
BglI	GCCNNNNNGGC	1 cut(s)	626
BglII	AGATCT	2 cut(s)	1540, 2458
BmcAI	AGTACT	1 cut(s)	453
Bme1390I	CCNGG	6 cut(s)	26, 225, 331, 1617, 2284, 2301
Bme18I	GGWCC	2 cut(s)	220, 1449
BmeT110I	CYCGRG	2 cut(s)	158, 309
BmgT120I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
BmrFI	CCNGG	6 cut(s)	26, 225, 331, 1617, 2284, 2301
BmrI	ACTGGG	1 cut(s)	1113
BmsI	GCATC	3 cut(s)	302, 316, 844
BmuI	ACTGGG	1 cut(s)	1113
BoxI	GACNNNNGTC	2 cut(s)	43, 80
BpiI	GAAGAC	3 cut(s)	76, 539, 2070
BpmI	CTGGAG	1 cut(s)	214
Bpu10I	CCTNAGC	1 cut(s)	719
Bpu14I	TTCGAA	1 cut(s)	1429
BpuEI	CTTGAG	2 cut(s)	393, 918
BpuMI	CCSGG	2 cut(s)	225, 2284
BsaBI	GATNNNNATC	1 cut(s)	702
BsaHI	GRCGYC	1 cut(s)	2235
BsaI	GGTCTC	1 cut(s)	1079
BsaJI	CCNNGG	9 cut(s)	24, 25, 223, 238, 330, 1069, 1615, 2248, 2328
BsaXI	ACNNNNNCTCC	6 cut(s)	212, 242, 2127, 2157, 2214, 2244
Bsc4I	CCNNNNNNNGG	5 cut(s)	17, 732, 1054, 1127, 1152
Bse1I	ACTGG	5 cut(s)	662, 1108, 1467, 1892, 2290
Bse8I	GATNNNNATC	1 cut(s)	702
BseBI	CCWGG	4 cut(s)	26, 331, 1617, 2301
BseDI	CCNNGG	9 cut(s)	24, 25, 223, 238, 330, 1069, 1615, 2248, 2328
BseGI	GGATG	3 cut(s)	808, 1141, 1534
BseJI	GATNNNNATC	1 cut(s)	702
BseLI	CCNNNNNNNGG	5 cut(s)	17, 732, 1054, 1127, 1152
BseMII	CTCAG	3 cut(s)	489, 1640, 2432
BseNI	ACTGG	5 cut(s)	662, 1108, 1467, 1892, 2290
BseRI	GAGGAG	2 cut(s)	122, 1212
BseSI	GKGCMC	2 cut(s)	2158, 2328
BseX3I	CGGCCG	2 cut(s)	41, 235
BseXI	GCAGC	8 cut(s)	283, 521, 613, 616, 688, 1016, 1577, 2392
BsgI	GTGCAG	1 cut(s)	615
Bsh1236I	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
Bsh1285I	CGRYCG	2 cut(s)	44, 238
BshFI	GGCC	8 cut(s)	21, 43, 237, 620, 661, 725, 1145, 1297
BshNI	GGYRCC	2 cut(s)	2155, 2234
BsiEI	CGRYCG	2 cut(s)	44, 238
BsiHKAI	GWGCWC	1 cut(s)	409
BsiHKCI	CYCGRG	2 cut(s)	158, 309
BsiSI	CCGG	3 cut(s)	18, 225, 2284
BslFI	GGGAC	1 cut(s)	86
BslI	CCNNNNNNNGG	5 cut(s)	17, 732, 1054, 1127, 1152
BsmAI	GTCTC	3 cut(s)	1079, 2149, 2298
BsmFI	GGGAC	1 cut(s)	86
BsmI	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2306
BsnI	GGCC	8 cut(s)	21, 43, 237, 620, 661, 725, 1145, 1297
Bso31I	GGTCTC	1 cut(s)	1079
BsoBI	CYCGRG	2 cut(s)	158, 309
Bsp119I	TTCGAA	1 cut(s)	1429
Bsp1286I	GDGCHC	5 cut(s)	159, 409, 1361, 2158, 2328
Bsp143I	GATC	8 cut(s)	364, 703, 841, 1438, 1540, 2242, 2279, 2458
Bsp19I	CCATGG	1 cut(s)	1069
BspANI	GGCC	8 cut(s)	21, 43, 237, 620, 661, 725, 1145, 1297
BspCNI	CTCAG	3 cut(s)	490, 1641, 2431
BspFNI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BspMAI	CTGCAG	1 cut(s)	273
BspPI	GGATC	2 cut(s)	711, 2274
BspT104I	TTCGAA	1 cut(s)	1429
BspT107I	GGYRCC	2 cut(s)	2155, 2234
BspTI	CTTAAG	1 cut(s)	1763
BspTNI	GGTCTC	1 cut(s)	1079
BsrBI	CCGCTC	1 cut(s)	130
BsrI	ACTGG	5 cut(s)	662, 1108, 1467, 1892, 2290
BssECI	CCNNGG	9 cut(s)	24, 25, 223, 238, 330, 1069, 1615, 2248, 2328
BssMI	GATC	8 cut(s)	364, 703, 841, 1438, 1540, 2242, 2279, 2458
BssNAI	GTATAC	1 cut(s)	1980
BssNI	GRCGYC	1 cut(s)	2235
BssSI	CACGAG	2 cut(s)	645, 1332
BssT1I	CCWWGG	1 cut(s)	1069
Bst1107I	GTATAC	1 cut(s)	1980
Bst2BI	CACGAG	2 cut(s)	645, 1332
Bst2UI	CCWGG	4 cut(s)	26, 331, 1617, 2301
Bst4CI	ACNGT	5 cut(s)	591, 636, 1178, 1755, 2020
Bst6I	CTCTTC	1 cut(s)	1936
BstACI	GRCGYC	1 cut(s)	2235
BstAFI	CTTAAG	1 cut(s)	1763
BstAPI	GCANNNNNTGC	1 cut(s)	2414
BstBI	TTCGAA	1 cut(s)	1429
BstC8I	GCNNGC	2 cut(s)	2008, 2273
BstDEI	CTNAG	5 cut(s)	498, 719, 1649, 2276, 2418
BstDSI	CCRYGG	2 cut(s)	238, 1069
BstENI	CCTNNNNNAGG	1 cut(s)	1150
BstF5I	GGATG	3 cut(s)	808, 1141, 1534
BstFNI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BstH2I	RGCGCY	3 cut(s)	57, 166, 2238
BstHHI	GCGC	4 cut(s)	56, 165, 211, 2237
BstKTI	GATC	8 cut(s)	367, 706, 844, 1441, 1543, 2245, 2282, 2461
BstMAI	GTCTC	3 cut(s)	1079, 2149, 2298
BstMBI	GATC	8 cut(s)	364, 703, 841, 1438, 1540, 2242, 2279, 2458
BstMCI	CGRYCG	2 cut(s)	44, 238
BstMWI	GCNNNNNNNGC	8 cut(s)	122, 234, 271, 626, 629, 854, 1472, 2414
BstNI	CCWGG	4 cut(s)	26, 331, 1617, 2301
BstNSI	RCATGY	1 cut(s)	2275
BstPAI	GACNNNNGTC	2 cut(s)	43, 80
BstSCI	CCNGG	6 cut(s)	24, 223, 329, 1615, 2282, 2299
BstSFI	CTRYAG	2 cut(s)	269, 1469
BstSLI	GKGCMC	2 cut(s)	2158, 2328
BstUI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BstV1I	GCAGC	8 cut(s)	283, 521, 613, 616, 688, 1016, 1577, 2392
BstV2I	GAAGAC	3 cut(s)	76, 539, 2070
BstX2I	RGATCY	4 cut(s)	703, 1540, 2279, 2458
BstYI	RGATCY	4 cut(s)	703, 1540, 2279, 2458
BstZ17I	GTATAC	1 cut(s)	1980
BstZI	CGGCCG	2 cut(s)	41, 235
BsuRI	GGCC	8 cut(s)	21, 43, 237, 620, 661, 725, 1145, 1297
BtgI	CCRYGG	2 cut(s)	238, 1069
BtgZI	GCGATG	1 cut(s)	97
BtsCI	GGATG	3 cut(s)	808, 1141, 1534
BtsIMutI	CAGTG	2 cut(s)	632, 1101
Cac8I	GCNNGC	2 cut(s)	2008, 2273
CaiI	CAGNNNCTG	1 cut(s)	31
CfoI	GCGC	4 cut(s)	56, 165, 211, 2237
Cfr13I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
CseI	GACGC	4 cut(s)	43, 127, 160, 1567
CsiI	ACCWGGT	1 cut(s)	2299
Csp6I	GTAC	3 cut(s)	452, 950, 2262
CviQI	GTAC	3 cut(s)	452, 950, 2262
DdeI	CTNAG	5 cut(s)	498, 719, 1649, 2276, 2418
DinI	GGCGCC	1 cut(s)	2236
DpnI	GATC	8 cut(s)	366, 705, 843, 1440, 1542, 2244, 2281, 2460
DpnII	GATC	8 cut(s)	364, 703, 841, 1438, 1540, 2242, 2279, 2458
DraI	TTTAAA	3 cut(s)	654, 1254, 1324
DrdI	GACNNNNNNGTC	1 cut(s)	95
DseDI	GACNNNNNNGTC	1 cut(s)	95
EaeI	YGGCCR	3 cut(s)	41, 235, 659
EagI	CGGCCG	2 cut(s)	41, 235
Eam1104I	CTCTTC	1 cut(s)	1936
EarI	CTCTTC	1 cut(s)	1936
EciI	GGCGGA	1 cut(s)	97
Ecl136II	GAGCTC	1 cut(s)	407
EclXI	CGGCCG	2 cut(s)	41, 235
Eco130I	CCWWGG	1 cut(s)	1069
Eco147I	AGGCCT	1 cut(s)	1145
Eco24I	GRGCYC	3 cut(s)	159, 409, 1361
Eco31I	GGTCTC	1 cut(s)	1079
Eco32I	GATATC	1 cut(s)	1622
Eco47I	GGWCC	2 cut(s)	220, 1449
Eco52I	CGGCCG	2 cut(s)	41, 235
Eco53kI	GAGCTC	1 cut(s)	407
Eco57I	CTGAAG	3 cut(s)	1568, 1682, 2367
Eco88I	CYCGRG	2 cut(s)	158, 309
EcoICRI	GAGCTC	1 cut(s)	407
EcoNI	CCTNNNNNAGG	1 cut(s)	1150
EcoRII	CCWGG	4 cut(s)	24, 329, 1615, 2299
EcoRV	GATATC	1 cut(s)	1622
EcoT14I	CCWWGG	1 cut(s)	1069
EcoT22I	ATGCAT	2 cut(s)	295, 1953
EcoT38I	GRGCYC	3 cut(s)	159, 409, 1361
EgeI	GGCGCC	1 cut(s)	2236
EheI	GGCGCC	1 cut(s)	2236
ErhI	CCWWGG	1 cut(s)	1069
FaqI	GGGAC	1 cut(s)	86
FauI	CCCGC	1 cut(s)	452
FauNDI	CATATG	2 cut(s)	992, 1060
FbaI	TGATCA	1 cut(s)	364
FblI	GTMKAC	2 cut(s)	399, 1979
FokI	GGATG	3 cut(s)	795, 1148, 1541
FriOI	GRGCYC	3 cut(s)	159, 409, 1361
FspBI	CTAG	3 cut(s)	516, 2267, 2321
GlaI	GCGC	4 cut(s)	55, 164, 210, 2236
GsuI	CTGGAG	1 cut(s)	214
HaeII	RGCGCY	3 cut(s)	57, 166, 2238
HaeIII	GGCC	8 cut(s)	21, 43, 237, 620, 661, 725, 1145, 1297
HapII	CCGG	3 cut(s)	18, 225, 2284
HgaI	GACGC	4 cut(s)	43, 127, 160, 1567
HhaI	GCGC	4 cut(s)	56, 165, 211, 2237
Hin1I	GRCGYC	1 cut(s)	2235
Hin6I	GCGC	4 cut(s)	54, 163, 209, 2235
HinP1I	GCGC	4 cut(s)	54, 163, 209, 2235
HincII	GTYRAC	2 cut(s)	466, 2340
HindII	GTYRAC	2 cut(s)	466, 2340
HindIII	AAGCTT	3 cut(s)	343, 833, 869
HinfI	GANTC	6 cut(s)	14, 572, 1727, 2077, 2257, 2352
HpaI	GTTAAC	1 cut(s)	466
HpaII	CCGG	3 cut(s)	18, 225, 2284
HphI	GGTGA	2 cut(s)	1010, 1497
Hpy166II	GTNNAC	7 cut(s)	400, 466, 1449, 1638, 1980, 2016, 2340
Hpy188I	TCNGA	8 cut(s)	325, 475, 1276, 1587, 1662, 1885, 2252, 2332
Hpy8I	GTNNAC	7 cut(s)	400, 466, 1449, 1638, 1980, 2016, 2340
Hpy99I	CGWCG	9 cut(s)	42, 64, 67, 70, 79, 143, 176, 1869, 2085
HpyAV	CCTTC	7 cut(s)	199, 1131, 1150, 1195, 1592, 1729, 1979
HpyCH4III	ACNGT	5 cut(s)	591, 636, 1178, 1755, 2020
HpyCH4IV	ACGT	3 cut(s)	68, 750, 890
HpyF10VI	GCNNNNNNNGC	8 cut(s)	122, 234, 271, 626, 629, 854, 1472, 2414
HpyF3I	CTNAG	5 cut(s)	498, 719, 1649, 2276, 2418
HpySE526I	ACGT	3 cut(s)	68, 750, 890
Hsp92I	GRCGYC	1 cut(s)	2235
HspAI	GCGC	4 cut(s)	54, 163, 209, 2235
KasI	GGCGCC	1 cut(s)	2234
Ksp22I	TGATCA	1 cut(s)	364
KspAI	GTTAAC	1 cut(s)	466
Kzo9I	GATC	8 cut(s)	364, 703, 841, 1438, 1540, 2242, 2279, 2458
Lsp1109I	GCAGC	8 cut(s)	283, 521, 613, 616, 688, 1016, 1577, 2392
LweI	GCATC	3 cut(s)	302, 316, 844
MabI	ACCWGGT	1 cut(s)	2299
MaeI	CTAG	3 cut(s)	516, 2267, 2321
MaeII	ACGT	3 cut(s)	68, 750, 890
MaeIII	GTNAC	4 cut(s)	45, 1099, 1334, 1917
MalI	GATC	8 cut(s)	366, 705, 843, 1440, 1542, 2244, 2281, 2460
MbiI	CCGCTC	1 cut(s)	130
MboI	GATC	8 cut(s)	364, 703, 841, 1438, 1540, 2242, 2279, 2458
MflI	RGATCY	4 cut(s)	703, 1540, 2279, 2458
MhlI	GDGCHC	5 cut(s)	159, 409, 1361, 2158, 2328
MlsI	TGGCCA	1 cut(s)	661
MluI	ACGCGT	1 cut(s)	195
MluNI	TGGCCA	1 cut(s)	661
Mly113I	GGCGCC	1 cut(s)	2235
MmeI	TCCRAC	1 cut(s)	1918
Mox20I	TGGCCA	1 cut(s)	661
Mph1103I	ATGCAT	2 cut(s)	295, 1953
MscI	TGGCCA	1 cut(s)	661
MslI	CAYNNNNRTG	1 cut(s)	2130
Msp20I	TGGCCA	1 cut(s)	661
MspA1I	CMGCKG	3 cut(s)	234, 623, 1510
MspCI	CTTAAG	1 cut(s)	1763
MspI	CCGG	3 cut(s)	18, 225, 2284
MspR9I	CCNGG	6 cut(s)	26, 225, 331, 1617, 2284, 2301
MssI	GTTTAAAC	1 cut(s)	654
Mva1269I	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2306
MvaI	CCWGG	4 cut(s)	26, 331, 1617, 2301
MvnI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
MwoI	GCNNNNNNNGC	8 cut(s)	122, 234, 271, 626, 629, 854, 1472, 2414
NarI	GGCGCC	1 cut(s)	2235
NciI	CCSGG	2 cut(s)	225, 2284
NcoI	CCATGG	1 cut(s)	1069
NdeI	CATATG	2 cut(s)	992, 1060
NdeII	GATC	8 cut(s)	364, 703, 841, 1438, 1540, 2242, 2279, 2458
NmuCI	GTSAC	4 cut(s)	45, 1099, 1334, 1917
NsiI	ATGCAT	2 cut(s)	295, 1953
NspI	RCATGY	1 cut(s)	2275
NspV	TTCGAA	1 cut(s)	1429
PaeI	GCATGC	1 cut(s)	2275
PaeR7I	CTCGAG	1 cut(s)	309
PasI	CCCWGGG	1 cut(s)	25
PceI	AGGCCT	1 cut(s)	1145
PcsI	WCGNNNNNNNCGW	2 cut(s)	41, 71
PctI	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2306
PfeI	GAWTC	6 cut(s)	14, 572, 1727, 2077, 2257, 2352
PflMI	CCANNNNNTGG	1 cut(s)	1054
PluTI	GGCGCC	1 cut(s)	2238
PmeI	GTTTAAAC	1 cut(s)	654
PshAI	GACNNNNGTC	2 cut(s)	43, 80
PshBI	ATTAAT	1 cut(s)	2088
PsiI	TTATAA	1 cut(s)	1163
Psp124BI	GAGCTC	1 cut(s)	409
Psp6I	CCWGG	4 cut(s)	24, 329, 1615, 2299
PspGI	CCWGG	4 cut(s)	24, 329, 1615, 2299
PspPI	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
PspXI	VCTCGAGB	1 cut(s)	309
PstI	CTGCAG	1 cut(s)	273
PstNI	CAGNNNCTG	1 cut(s)	31
PsuI	RGATCY	4 cut(s)	703, 1540, 2279, 2458
RsaI	GTAC	3 cut(s)	453, 951, 2263
RsaNI	GTAC	3 cut(s)	452, 950, 2262
RseI	CAYNNNNRTG	1 cut(s)	2130
SacI	GAGCTC	1 cut(s)	409
Sau3AI	GATC	8 cut(s)	364, 703, 841, 1438, 1540, 2242, 2279, 2458
Sau96I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
ScaI	AGTACT	1 cut(s)	453
ScrFI	CCNGG	6 cut(s)	26, 225, 331, 1617, 2284, 2301
SduI	GDGCHC	5 cut(s)	159, 409, 1361, 2158, 2328
SexAI	ACCWGGT	1 cut(s)	2299
SfaNI	GCATC	3 cut(s)	302, 316, 844
SfcI	CTRYAG	2 cut(s)	269, 1469
SfoI	GGCGCC	1 cut(s)	2236
Sfr274I	CTCGAG	1 cut(s)	309
SfuI	TTCGAA	1 cut(s)	1429
SinI	GGWCC	2 cut(s)	220, 1449
SlaI	CTCGAG	1 cut(s)	309
SmiI	ATTTAAAT	1 cut(s)	1254
SmiMI	CAYNNNNRTG	1 cut(s)	2130
SmlI	CTYRAG	4 cut(s)	309, 408, 933, 1763
SmoI	CTYRAG	4 cut(s)	309, 408, 933, 1763
SphI	GCATGC	1 cut(s)	2275
SseBI	AGGCCT	1 cut(s)	1145
SspDI	GGCGCC	1 cut(s)	2234
SspI	AATATT	4 cut(s)	359, 559, 1262, 1657
SspMI	CTAG	3 cut(s)	516, 2267, 2321
SstI	GAGCTC	1 cut(s)	409
StuI	AGGCCT	1 cut(s)	1145
StyD4I	CCNGG	6 cut(s)	24, 223, 329, 1615, 2282, 2299
StyI	CCWWGG	1 cut(s)	1069
SwaI	ATTTAAAT	1 cut(s)	1254
TaaI	ACNGT	5 cut(s)	591, 636, 1178, 1755, 2020
TaiI	ACGT	3 cut(s)	71, 753, 893
TatI	WGTACW	2 cut(s)	451, 949
TauI	GCSGC	6 cut(s)	8, 59, 237, 515, 623, 1209
TfiI	GAWTC	6 cut(s)	14, 572, 1727, 2077, 2257, 2352
TscAI	CASTG	2 cut(s)	639, 1108
TseFI	GTSAC	4 cut(s)	45, 1099, 1334, 1917
TseI	GCWGC	8 cut(s)	271, 509, 601, 629, 676, 1004, 1565, 2405
Tsp45I	GTSAC	4 cut(s)	45, 1099, 1334, 1917
TspGWI	ACGGA	4 cut(s)	64, 92, 551, 1853
TspRI	CASTG	2 cut(s)	639, 1108
Van91I	CCANNNNNTGG	1 cut(s)	1054
Vha464I	CTTAAG	1 cut(s)	1763
VpaK11BI	GGWCC	2 cut(s)	220, 1449
VspI	ATTAAT	1 cut(s)	2088
XagI	CCTNNNNNAGG	1 cut(s)	1150
XapI	RAATTY	6 cut(s)	899, 1825, 1879, 2105, 2190, 2369
XceI	RCATGY	1 cut(s)	2275
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2297
XhoI	CTCGAG	1 cut(s)	309
XmiI	GTMKAC	2 cut(s)	399, 1979
XspI	CTAG	3 cut(s)	516, 2267, 2321
ZrmI	AGTACT	1 cut(s)	453
Zsp2I	ATGCAT	2 cut(s)	295, 1953

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.