Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Forward (+)

1331491 .. 1338728

Gene Structure 7238 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1004100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3279 bp

ATGGATTTGCTGCTAGTTCCGTATTCATCAGATCCCAGCCTGAAGATAATTCAGTGGCCGCCCTTCTTGCTTGCTAGCAAAATCCCAATAGCATTAGATATGGCAGTTCAATTTAAATCGAAGGACTCCGATCTCTGGAAGCGGATATGTGCGGATGAATATATGAAATGTGCTGTGATTGAATGTTATGAATCTTTCAAACATGTCCTTAATACTCTGGTAGTTGGAGACAATGAGAAAAGGATAATTGGCATCATCGTGAAGGAAATTGAAAGTAACATTTCAAAGAATACATTTCTTGTAAATTTTAGAATGGGTTCCTTGCCCACTCTTTGCAAGAAATTTGTAGAGCTTGTGGGGATCTTGAAAGATGGTGATGCATTCAAGCGATCTTCAGTGGTGTTGTTGCTTCAAGATATGCTGGAAGTAGTCACGCGTGATATGATGGTGAATGAGATCCGAGAGTTGGTTGAAGTTGGTCATAGTAGCAAGGATGCTGGAAGGCAACTCTTTGCTGGAACTGATGCAAAACCTGCAATATTGTTCCCTCCTCCAGTTACAGCACAGTGGGAAGAACAGATCAGACGCCTCCATCTGCTGCTAACAGTTAAGGAATCTGCCATTGACGTACCAACAAACCTAGAAGCACGTAGAAGGATTGCATTTTTTACAAATTCATTGTTCATGGATATGCCTCGTGCTCCCCGCGTCCGTAAAATGCTTTCATTTAGCATCATGACGCCATACTATAGTGAGGAGACTCTGTATTCCAAATCGGACCTTGAGATGGAAAATGAAGACGGTGTATCAATCATATACTACTTACAGAAGATTTTCCCAGATGAATGGAATAACTTCATGGAGCGACTTAATTGTAAAAAGGATAGTGAAATATGGGAGAATGAAGAAAATGTTTTGCAGCTTCGTCATTGGGTCTCCTTGCGAGGACAAACGCTCTGCAGGACTGTTAGAGGAATGATGTATTACCGACGAGCTCTGAAGCTTCAGGCTTTTCTTGACATGGCCAATGAAACCGAGATACTAGATGGCTACAAAGCAATCACGGTTCCACCAGAAGAAGAAAAGAAAAGCCAGAGATCTCTTTATGCTCAATTAGAAGCAGTGGCTGACCTTAAATTCACCTATGTTGCTACCTGCCAAAACTATGGCAATCAGAAACGCAGTGGAGATCGACGCGCAACAGACATCTTGAATCTGATGGTTAATTATCCTTCTCTTCGGGTTGCATATATCGATGAAGTTGAAGAGAGTGACGCTGCTAGTGGAAAGGTGCAAAAGGTTTACTATTCTGTCTTGGTCAAAGCTGTTGACAATCATGATCAGGAAATATATCGTATAAAGTTGCCTGGTTCGGCAAAGATTGGTGAAGGGAAACCCGAAAACCAGAACCATGCTATAATTTTTACTCGAGGAGAAGCTCTTCAGGCCATTGACATGAACCAGGACAATTACTTAGAAGAAGCTTTTAAAATGAGAAACCTTCTTGAAGAATTTAATGAGGACCACGGAGTACGTCCTCCTTCAATTTTAGGTGTTCGTGAGCATATCTTTACTGGAAGTGTTTCTTCATTGGCCTGGTTTATGTCAAATCAGGAAATGAGCTTTGTGACCATAGGTCAAAGAGTTCTTGCAAGACCTTTGAAGATCCGCTTCCACTATGGACACCCAGATGTCTTTGATAGAATCTTCCACATTACTCGTGGTGGCATGAGCAAGGCTTCCCGTGGCATCAATCTGAGCGAGGACATCTTTGCTGGTTTTAATTCAACGTTAAGGCGAGGGAATGTTACTCACCATGAATACATTCAAGTTGGGAAGGGTAGAGATGTTGGGCTCAACCAAATCTCTCTCTTTGAAGCCAAAGTTGCTTGTGGTAATGGAGAGCAAACGCTTAGCAGGGATATCTACCGCTTAGGGCACCGTTTTGACTTCTTTCGCATGTTGTCCTTCTATTTTTCGACAATAGGGTTTTACATCAGCGCAATGTTGGTTGTCCTTACAGTCTATGCGTACTTATACGGCAGACTTTACTTGTCATTAAGTGGAATGGAAAAGACAATTGTGAACTATGCCGCCACCAGGGGAAATAATGTTCTACAAGCAGCCATGGCTTCACAATCTATTTTCCAATTAGGTCTTTTGACTTCCTTGCCCATGATCATGGAAATTGGACTAGAAAGAGGGTTCAGAACTGCGTTAGGGGACATGATAATTATGCAGCTGCAACTAGCATCCGTTTTCTTCACCTTCTCTCTTGGTACCAAAGTTCATTATTATGGTCGAACTGTGCTGCACGGCGGTGCTAAATACAGAGCAACTGGTCGTGGCTTTGTTGTCAGGCATGAAAAGTTTGCCGAGAATTACAGGATGTACTCAAGGAGTCATTTTGTGAAAGGCCTTGAACTTATGGTACTGCTTATAATTTATCAGATATACGGTTCAGCAGTAACTGGTACAATATCATATATCTTCGTCACATTCTCCATGTGGTTCTTAGTGGTTTCGTGGCTGTTTGCTCCCTTCCTGTTTAACCCTTCAGGATTTGAATGGCAAAAGATAGTCGAAGATTGGGATGATTGGACAAAATGGATAAGTAGCCATGGTGGTATTGGTGTGCCAGCGACCAAGAGTTGGGAGTCTTGGTGGGATGAGGAACAGGAACACCTGCAGCACACTGGGGTTTTGGGACGATTTTGGGAGATTGTTCTTTCTCTACGCTTCTTTCTCTTCCAATACGGAATTGTGTACCATCTAAATGTGGCCAGGGGTGATAAAAGCATCATGGTTTATGGTCTGTCATGGCTGGTCATTGTCGCTGGGATGATCATCCTAAAGGTTGTGTCGATGGGAAGAAAGCGGTTCAGTGCAGATTTCCAGCTGATGTTCAGGCTTCTCAAGTTGTTCCTGTTTATTGGGTTTGTCGTCACTATTGGAATGCTTTTCGCTTTCCTCAGTCTTACTGTTGGTGACATCTTTGTGAGCTTATTGGCCTTCTTGCCTACAGGATGGGCGCTACTAATGATATCACAAGCATGCAAGCCGTTGGTGAAGGCCCTAGGAATGTGGGGATCTGTCAAAGCTCTAGCAAGAGGGTACGAGTACGTGATGGGGATCACTATACTTGCACCAGTAGTTGTTCTTGCATGGTTCCCATTTGTCTCGGAGTTCCAGACCAGGCTGCTATTCAACCAAGCTTTCAGCCGAGGGCTTCAGATCCAACGTATTCTTACTGGTGGGAAGAAGCACAAGTCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1093

Amino Acids

124.99

Weight (kDa)

8.88

Isoelectric Point (pI)

36.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CALS1	PF25968	20 - 203	1.4e-67	Callose synthase 1 domain
Glucan_synthase	PF02364	210 - 297	1e-26	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	301 - 904	2.3e-209	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2441
AarI	CACCTGC	1 cut(s)	2694
Acc36I	ACCTGC	3 cut(s)	541, 1163, 2694
Acc65I	GGTACC	1 cut(s)	2279
AccB1I	GGYRCC	2 cut(s)	1938, 2279
AccB7I	CCANNNNNTGG	1 cut(s)	2652
AccII	CGCG	3 cut(s)	436, 708, 1197
AciI	CCGC	9 cut(s)	59, 142, 152, 706, 1669, 1930, 2094, 2319, 2878
AclI	AACGTT	1 cut(s)	1790
AclWI	GGATC	7 cut(s)	26, 368, 451, 1660, 3097, 3140, 3229
AcoI	YGGCCR	3 cut(s)	56, 1023, 2781
AcsI	RAATTY	5 cut(s)	304, 341, 673, 1136, 1511
AcuI	CTGAAG	7 cut(s)	62, 378, 989, 1019, 1427, 2541, 3215
AcyI	GRCGYC	2 cut(s)	586, 740
AfiI	CCNNNNNNNGG	5 cut(s)	135, 466, 787, 2100, 2652
AflIII	ACRYGT	2 cut(s)	202, 434
AhdI	GACNNNNNGTC	1 cut(s)	1635
AjnI	CCWGG	6 cut(s)	1366, 1461, 1595, 2099, 2783, 3194
AleI	CACNNNNGTG	1 cut(s)	2319
AloI	GAACNNNNNNTCC	2 cut(s)	2097, 2129
Alw21I	GWGCWC	2 cut(s)	703, 997
Alw26I	GTCTC	4 cut(s)	222, 752, 940, 3184
AlwI	GGATC	7 cut(s)	26, 368, 451, 1660, 3097, 3140, 3229
AlwNI	CAGNNNCTG	2 cut(s)	1127, 2471
Ama87I	CYCGRG	1 cut(s)	1428
AoxI	GGCC	8 cut(s)	56, 1023, 1446, 1593, 2416, 2781, 3009, 3072
ApoI	RAATTY	5 cut(s)	304, 341, 673, 1136, 1511
Asp700I	GAANNNNTTC	6 cut(s)	316, 833, 854, 1440, 1582, 1824
Asp718I	GGTACC	1 cut(s)	2279
AspA2I	CCTAGG	1 cut(s)	3076
AspLEI	GCGC	3 cut(s)	1199, 2003, 3034
AspS9I	GGNCC	3 cut(s)	778, 1522, 3073
AsuHPI	GGTGA	9 cut(s)	386, 460, 1132, 1397, 1805, 2257, 2801, 2999, 3079
AsuNHI	GCTAGC	1 cut(s)	74
AvaI	CYCGRG	1 cut(s)	1428
AvaII	GGWCC	2 cut(s)	778, 1522
AvrII	CCTAGG	1 cut(s)	3076
BaeGI	GKGCMC	1 cut(s)	1941
BaeI	ACNNNNGTAYC	2 cut(s)	2271, 2304
BalI	TGGCCA	2 cut(s)	1025, 2783
BanI	GGYRCC	2 cut(s)	1938, 2279
BanII	GRGCYC	2 cut(s)	997, 1857
BauI	CACGAG	2 cut(s)	696, 1719
BbsI	GAAGAC	1 cut(s)	804
Bbv12I	GWGCWC	2 cut(s)	703, 997
BbvI	GCAGC	9 cut(s)	585, 931, 1264, 2135, 2229, 2251, 2298, 2701, 3186
BceAI	ACGGC	3 cut(s)	2056, 2332, 3046
BciT130I	CCWGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
BclI	TGATCA	3 cut(s)	1339, 2178, 2844
BcoDI	GTCTC	4 cut(s)	222, 752, 940, 3184
BfaI	CTAG	9 cut(s)	14, 75, 641, 1043, 1281, 2195, 2249, 3077, 3104
BfmI	CTRYAG	4 cut(s)	748, 958, 2687, 3021
BfoI	RGCGCY	1 cut(s)	3035
BfuAI	ACCTGC	3 cut(s)	541, 1163, 2694
BglII	AGATCT	1 cut(s)	1097
BlnI	CCTAGG	1 cut(s)	3076
BlpI	GCTNAGC	1 cut(s)	1913
Bme1390I	CCNGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
Bme18I	GGWCC	2 cut(s)	778, 1522
BmeRI	GACNNNNNGTC	1 cut(s)	1635
BmeT110I	CYCGRG	1 cut(s)	1428
BmgT120I	GGNCC	3 cut(s)	778, 1522, 3073
BmiI	GGNNCC	5 cut(s)	319, 1068, 1940, 2281, 3170
BmrFI	CCNGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
BmrI	ACTGGG	1 cut(s)	2706
BmsI	GCATC	8 cut(s)	261, 367, 484, 514, 741, 1758, 2261, 2808
BmtI	GCTAGC	1 cut(s)	78
BmuI	ACTGGG	1 cut(s)	2706
BpiI	GAAGAC	1 cut(s)	804
BpmI	CTGGAG	1 cut(s)	537
Bpu10I	CCTNAGC	1 cut(s)	1933
Bpu1102I	GCTNAGC	1 cut(s)	1913
BpuEI	CTTGAG	3 cut(s)	803, 2380, 2900
Bsa29I	ATCGAT	1 cut(s)	1254
BsaAI	YACGTR	2 cut(s)	650, 3124
BsaBI	GATNNNNATC	2 cut(s)	2846, 3131
BsaHI	GRCGYC	2 cut(s)	586, 740
BsaI	GGTCTC	1 cut(s)	940
BsaJI	CCNNGG	8 cut(s)	1525, 1744, 2100, 2127, 2620, 2784, 3076, 3223
BsaXI	ACNNNNNCTCC	4 cut(s)	749, 779, 2648, 2678
Bsc4I	CCNNNNNNNGG	5 cut(s)	135, 466, 787, 2100, 2652
Bse1I	ACTGG	7 cut(s)	554, 1579, 2344, 2476, 2701, 3149, 3256
Bse3DI	GCAATG	1 cut(s)	2010
Bse8I	GATNNNNATC	2 cut(s)	2846, 3131
BseBI	CCWGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
BseCI	ATCGAT	1 cut(s)	1254
BseDI	CCNNGG	8 cut(s)	1525, 1744, 2100, 2127, 2620, 2784, 3076, 3223
BseGI	GGATG	9 cut(s)	160, 499, 2252, 2394, 2599, 2674, 2847, 2847, 3032
BseJI	GATNNNNATC	2 cut(s)	2846, 3131
BseLI	CCNNNNNNNGG	5 cut(s)	135, 466, 787, 2100, 2652
BseMI	GCAATG	1 cut(s)	2010
BseMII	CTCAG	2 cut(s)	1748, 2986
BseNI	ACTGG	7 cut(s)	554, 1579, 2344, 2476, 2701, 3149, 3256
BseRI	GAGGAG	3 cut(s)	540, 770, 1446
BseSI	GKGCMC	1 cut(s)	1941
BseXI	GCAGC	9 cut(s)	585, 931, 1264, 2135, 2229, 2251, 2298, 2701, 3186
BseYI	CCCAGC	2 cut(s)	35, 2837
BsgI	GTGCAG	2 cut(s)	2297, 2907
Bsh1236I	CGCG	3 cut(s)	436, 708, 1197
BshFI	GGCC	8 cut(s)	58, 1025, 1448, 1595, 2418, 2783, 3011, 3074
BshNI	GGYRCC	2 cut(s)	1938, 2279
BshVI	ATCGAT	1 cut(s)	1254
BsiHKAI	GWGCWC	2 cut(s)	703, 997
BsiHKCI	CYCGRG	1 cut(s)	1428
BslFI	GGGAC	2 cut(s)	2237, 2721
BslI	CCNNNNNNNGG	5 cut(s)	135, 466, 787, 2100, 2652
BsmAI	GTCTC	4 cut(s)	222, 752, 940, 3184
BsmFI	GGGAC	2 cut(s)	2237, 2721
BsmI	GAATGC	2 cut(s)	380, 2961
BsnI	GGCC	8 cut(s)	58, 1025, 1448, 1595, 2418, 2783, 3011, 3074
Bso31I	GGTCTC	1 cut(s)	940
BsoBI	CYCGRG	1 cut(s)	1428
Bsp1286I	GDGCHC	4 cut(s)	703, 997, 1857, 1941
Bsp1720I	GCTNAGC	1 cut(s)	1913
Bsp19I	CCATGG	2 cut(s)	2127, 2620
BspACI	CCGC	9 cut(s)	59, 142, 152, 706, 1669, 1930, 2094, 2319, 2878
BspANI	GGCC	8 cut(s)	58, 1025, 1448, 1595, 2418, 2783, 3011, 3074
BspCNI	CTCAG	2 cut(s)	1749, 2985
BspDI	ATCGAT	1 cut(s)	1254
BspFNI	CGCG	3 cut(s)	436, 708, 1197
BspHI	TCATGA	2 cut(s)	735, 1336
BspLI	GGNNCC	5 cut(s)	319, 1068, 1940, 2281, 3170
BspMAI	CTGCAG	2 cut(s)	962, 2691
BspMI	ACCTGC	3 cut(s)	541, 1163, 2694
BspOI	GCTAGC	1 cut(s)	78
BspPI	GGATC	7 cut(s)	26, 368, 451, 1660, 3097, 3140, 3229
BspQI	GCTCTTC	1 cut(s)	1446
BspT107I	GGYRCC	2 cut(s)	1938, 2279
BspTNI	GGTCTC	1 cut(s)	940
BsrDI	GCAATG	1 cut(s)	2010
BsrI	ACTGG	7 cut(s)	554, 1579, 2344, 2476, 2701, 3149, 3256
BssECI	CCNNGG	8 cut(s)	1525, 1744, 2100, 2127, 2620, 2784, 3076, 3223
BssNI	GRCGYC	2 cut(s)	586, 740
BssSI	CACGAG	2 cut(s)	696, 1719
BssT1I	CCWWGG	3 cut(s)	2127, 2620, 3076
Bst2BI	CACGAG	2 cut(s)	696, 1719
Bst2UI	CCWGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
Bst6I	CTCTTC	4 cut(s)	1242, 1260, 1446, 2753
BstACI	GRCGYC	2 cut(s)	586, 740
BstAPI	GCANNNNNTGC	1 cut(s)	533
BstBAI	YACGTR	2 cut(s)	650, 3124
BstC8I	GCNNGC	5 cut(s)	72, 76, 2640, 3055, 3059
BstDEI	CTNAG	6 cut(s)	1474, 1757, 1913, 1933, 2515, 2972
BstDSI	CCRYGG	4 cut(s)	1525, 1744, 2127, 2620
BstF5I	GGATG	9 cut(s)	160, 499, 2252, 2394, 2599, 2674, 2847, 2847, 3032
BstFNI	CGCG	3 cut(s)	436, 708, 1197
BstH2I	RGCGCY	1 cut(s)	3035
BstHHI	GCGC	3 cut(s)	1199, 2003, 3034
BstMAI	GTCTC	4 cut(s)	222, 752, 940, 3184
BstMWI	GCNNNNNNNGC	5 cut(s)	67, 533, 1445, 1886, 2129
BstNI	CCWGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
BstNSI	RCATGY	3 cut(s)	206, 1963, 3057
BstSCI	CCNGG	6 cut(s)	1366, 1461, 1595, 2099, 2783, 3194
BstSFI	CTRYAG	4 cut(s)	748, 958, 2687, 3021
BstSLI	GKGCMC	1 cut(s)	1941
BstUI	CGCG	3 cut(s)	436, 708, 1197
BstV1I	GCAGC	9 cut(s)	585, 931, 1264, 2135, 2229, 2251, 2298, 2701, 3186
BstV2I	GAAGAC	1 cut(s)	804
BstX2I	RGATCY	7 cut(s)	31, 360, 456, 1097, 1665, 3089, 3234
BstXI	CCANNNNNNTGG	3 cut(s)	846, 1166, 2182
BstYI	RGATCY	7 cut(s)	31, 360, 456, 1097, 1665, 3089, 3234
Bsu15I	ATCGAT	1 cut(s)	1254
BsuRI	GGCC	8 cut(s)	58, 1025, 1448, 1595, 2418, 2783, 3011, 3074
BsuTUI	ATCGAT	1 cut(s)	1254
BtgI	CCRYGG	4 cut(s)	1525, 1744, 2127, 2620
BtsCI	GGATG	9 cut(s)	160, 499, 2252, 2394, 2599, 2674, 2847, 2847, 3032
BtsI	GCAGTG	2 cut(s)	1128, 1189
BtsIMutI	CAGTG	7 cut(s)	59, 402, 572, 1128, 1189, 2694, 2890
BveI	ACCTGC	3 cut(s)	541, 1163, 2694
Cac8I	GCNNGC	5 cut(s)	72, 76, 2640, 3055, 3059
CaiI	CAGNNNCTG	2 cut(s)	1127, 2471
CciI	TCATGA	2 cut(s)	735, 1336
CfoI	GCGC	3 cut(s)	1199, 2003, 3034
Cfr13I	GGNCC	3 cut(s)	778, 1522, 3073
ClaI	ATCGAT	1 cut(s)	1254
CseI	GACGC	5 cut(s)	594, 697, 748, 1203, 1283
DdeI	CTNAG	6 cut(s)	1474, 1757, 1913, 1933, 2515, 2972
DraI	TTTAAA	2 cut(s)	115, 1489
DriI	GACNNNNNGTC	1 cut(s)	1635
EaeI	YGGCCR	3 cut(s)	56, 1023, 2781
Eam1104I	CTCTTC	4 cut(s)	1242, 1260, 1446, 2753
Eam1105I	GACNNNNNGTC	1 cut(s)	1635
EarI	CTCTTC	4 cut(s)	1242, 1260, 1446, 2753
Ecl136II	GAGCTC	1 cut(s)	995
Eco130I	CCWWGG	3 cut(s)	2127, 2620, 3076
Eco147I	AGGCCT	1 cut(s)	2418
Eco24I	GRGCYC	2 cut(s)	997, 1857
Eco31I	GGTCTC	1 cut(s)	940
Eco32I	GATATC	2 cut(s)	1924, 3045
Eco47I	GGWCC	2 cut(s)	778, 1522
Eco53kI	GAGCTC	1 cut(s)	995
Eco57I	CTGAAG	7 cut(s)	62, 378, 989, 1019, 1427, 2541, 3215
Eco88I	CYCGRG	1 cut(s)	1428
EcoICRI	GAGCTC	1 cut(s)	995
EcoO109I	RGGNCCY	1 cut(s)	3073
EcoRII	CCWGG	6 cut(s)	1366, 1461, 1595, 2099, 2783, 3194
EcoRV	GATATC	2 cut(s)	1924, 3045
EcoT14I	CCWWGG	3 cut(s)	2127, 2620, 3076
EcoT22I	ATGCAT	1 cut(s)	382
EcoT38I	GRGCYC	2 cut(s)	997, 1857
ErhI	CCWWGG	3 cut(s)	2127, 2620, 3076
FalI	AAGNNNNNCTT	4 cut(s)	1570, 1602, 1655, 1687
FaqI	GGGAC	2 cut(s)	2237, 2721
FauI	CCCGC	1 cut(s)	713
FbaI	TGATCA	3 cut(s)	1339, 2178, 2844
FokI	GGATG	9 cut(s)	167, 506, 2239, 2401, 2606, 2681, 2834, 2854, 3039
FriOI	GRGCYC	2 cut(s)	997, 1857
FspBI	CTAG	9 cut(s)	14, 75, 641, 1043, 1281, 2195, 2249, 3077, 3104
GlaI	GCGC	3 cut(s)	1198, 2002, 3033
GsaI	CCCAGC	2 cut(s)	39, 2841
GsuI	CTGGAG	1 cut(s)	537
HaeII	RGCGCY	1 cut(s)	3035
HaeIII	GGCC	8 cut(s)	58, 1025, 1448, 1595, 2418, 2783, 3011, 3074
HgaI	GACGC	5 cut(s)	594, 697, 748, 1203, 1283
HhaI	GCGC	3 cut(s)	1199, 2003, 3034
Hin1I	GRCGYC	2 cut(s)	586, 740
Hin6I	GCGC	3 cut(s)	1197, 2001, 3032
HinP1I	GCGC	3 cut(s)	1197, 2001, 3032
HincII	GTYRAC	1 cut(s)	1330
HindII	GTYRAC	1 cut(s)	1330
HindIII	AAGCTT	3 cut(s)	1001, 1482, 3213
HinfI	GANTC	8 cut(s)	125, 191, 614, 760, 1213, 1704, 2401, 2657
HphI	GGTGA	9 cut(s)	386, 460, 1132, 1397, 1805, 2257, 2801, 2999, 3079
Hpy166II	GTNNAC	4 cut(s)	1303, 1330, 2086, 2767
Hpy8I	GTNNAC	4 cut(s)	1303, 1330, 2086, 2767
Hpy99I	CGWCG	2 cut(s)	993, 1197
HpyCH4IV	ACGT	6 cut(s)	627, 649, 1534, 1790, 3123, 3241
HpyF10VI	GCNNNNNNNGC	5 cut(s)	67, 533, 1445, 1886, 2129
HpyF3I	CTNAG	6 cut(s)	1474, 1757, 1913, 1933, 2515, 2972
HpySE526I	ACGT	6 cut(s)	627, 649, 1534, 1790, 3123, 3241
Hsp92I	GRCGYC	2 cut(s)	586, 740
HspAI	GCGC	3 cut(s)	1197, 2001, 3032
KpnI	GGTACC	1 cut(s)	2283
Ksp22I	TGATCA	3 cut(s)	1339, 2178, 2844
LguI	GCTCTTC	1 cut(s)	1446
LmnI	GCTCC	3 cut(s)	706, 862, 2542
Lsp1109I	GCAGC	9 cut(s)	585, 931, 1264, 2135, 2229, 2251, 2298, 2701, 3186
LweI	GCATC	8 cut(s)	261, 367, 484, 514, 741, 1758, 2261, 2808
MaeI	CTAG	9 cut(s)	14, 75, 641, 1043, 1281, 2195, 2249, 3077, 3104
MaeII	ACGT	6 cut(s)	627, 649, 1534, 1790, 3123, 3241
MfeI	CAATTG	1 cut(s)	2079
MflI	RGATCY	7 cut(s)	31, 360, 456, 1097, 1665, 3089, 3234
MhlI	GDGCHC	4 cut(s)	703, 997, 1857, 1941
MlsI	TGGCCA	2 cut(s)	1025, 2783
MluI	ACGCGT	1 cut(s)	434
MluNI	TGGCCA	2 cut(s)	1025, 2783
MlyI	GAGTC	4 cut(s)	119, 754, 2410, 2666
MmeI	TCCRAC	2 cut(s)	205, 3262
Mox20I	TGGCCA	2 cut(s)	1025, 2783
Mph1103I	ATGCAT	1 cut(s)	382
MroXI	GAANNNNTTC	6 cut(s)	316, 833, 854, 1440, 1582, 1824
MscI	TGGCCA	2 cut(s)	1025, 2783
Msp20I	TGGCCA	2 cut(s)	1025, 2783
MspA1I	CMGCKG	2 cut(s)	2242, 2899
MspR9I	CCNGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
MunI	CAATTG	1 cut(s)	2079
Mva1269I	GAATGC	2 cut(s)	380, 2961
MvaI	CCWGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
MvnI	CGCG	3 cut(s)	436, 708, 1197
MwoI	GCNNNNNNNGC	5 cut(s)	67, 533, 1445, 1886, 2129
NcoI	CCATGG	2 cut(s)	2127, 2620
NheI	GCTAGC	1 cut(s)	74
NlaIV	GGNNCC	5 cut(s)	319, 1068, 1940, 2281, 3170
NmeAIII	GCCGAG	2 cut(s)	2401, 3248
NmuCI	GTSAC	6 cut(s)	430, 1271, 1627, 2494, 2944, 2987
NsiI	ATGCAT	1 cut(s)	382
NspI	RCATGY	3 cut(s)	206, 1963, 3057
OliI	CACNNNNGTG	1 cut(s)	2319
PaeI	GCATGC	1 cut(s)	3057
PaeR7I	CTCGAG	1 cut(s)	1428
PagI	TCATGA	2 cut(s)	735, 1336
PaqCI	CACCTGC	1 cut(s)	2694
PceI	AGGCCT	1 cut(s)	2418
PciI	ACATGT	1 cut(s)	202
PciSI	GCTCTTC	1 cut(s)	1446
PctI	GAATGC	2 cut(s)	380, 2961
PdmI	GAANNNNTTC	6 cut(s)	316, 833, 854, 1440, 1582, 1824
PfeI	GAWTC	4 cut(s)	191, 614, 1213, 1704
PflMI	CCANNNNNTGG	1 cut(s)	2652
PleI	GAGTC	4 cut(s)	119, 754, 2409, 2665
PpsI	GAGTC	4 cut(s)	119, 754, 2409, 2665
Ppu21I	YACGTR	2 cut(s)	650, 3124
PscI	ACATGT	1 cut(s)	202
PsiI	TTATAA	1 cut(s)	2441
Psp124BI	GAGCTC	1 cut(s)	997
Psp1406I	AACGTT	1 cut(s)	1790
Psp6I	CCWGG	6 cut(s)	1366, 1461, 1595, 2099, 2783, 3194
PspFI	CCCAGC	2 cut(s)	35, 2837
PspGI	CCWGG	6 cut(s)	1366, 1461, 1595, 2099, 2783, 3194
PspN4I	GGNNCC	5 cut(s)	319, 1068, 1940, 2281, 3170
PspPI	GGNCC	3 cut(s)	778, 1522, 3073
PspXI	VCTCGAGB	1 cut(s)	1428
PsrI	GAACNNNNNNTAC	2 cut(s)	2415, 2447
PstI	CTGCAG	2 cut(s)	962, 2691
PstNI	CAGNNNCTG	2 cut(s)	1127, 2471
PsuI	RGATCY	7 cut(s)	31, 360, 456, 1097, 1665, 3089, 3234
PvuII	CAGCTG	2 cut(s)	2242, 2899
SacI	GAGCTC	1 cut(s)	997
SapI	GCTCTTC	1 cut(s)	1446
Sau96I	GGNCC	3 cut(s)	778, 1522, 3073
SchI	GAGTC	4 cut(s)	119, 754, 2410, 2666
ScrFI	CCNGG	6 cut(s)	1368, 1463, 1597, 2101, 2785, 3196
SduI	GDGCHC	4 cut(s)	703, 997, 1857, 1941
SfaNI	GCATC	8 cut(s)	261, 367, 484, 514, 741, 1758, 2261, 2808
SfcI	CTRYAG	4 cut(s)	748, 958, 2687, 3021
Sfr274I	CTCGAG	1 cut(s)	1428
SinI	GGWCC	2 cut(s)	778, 1522
SlaI	CTCGAG	1 cut(s)	1428
SmiI	ATTTAAAT	1 cut(s)	115
SmlI	CTYRAG	4 cut(s)	782, 1428, 2395, 2915
SmoI	CTYRAG	4 cut(s)	782, 1428, 2395, 2915
SphI	GCATGC	1 cut(s)	3057
SseBI	AGGCCT	1 cut(s)	2418
SsiI	CCGC	9 cut(s)	59, 142, 152, 706, 1669, 1930, 2094, 2319, 2878
SspI	AATATT	1 cut(s)	540
SspMI	CTAG	9 cut(s)	14, 75, 641, 1043, 1281, 2195, 2249, 3077, 3104
SstI	GAGCTC	1 cut(s)	997
StuI	AGGCCT	1 cut(s)	2418
StyD4I	CCNGG	6 cut(s)	1366, 1461, 1595, 2099, 2783, 3194
StyI	CCWWGG	3 cut(s)	2127, 2620, 3076
SwaI	ATTTAAAT	1 cut(s)	115
TaiI	ACGT	6 cut(s)	630, 652, 1537, 1793, 3126, 3244
TaqI	TCGA	8 cut(s)	119, 1192, 1254, 1429, 1979, 2302, 2583, 2864
TatI	WGTACW	1 cut(s)	2391
TauI	GCSGC	2 cut(s)	61, 2096
TfiI	GAWTC	4 cut(s)	191, 614, 1213, 1704
TscAI	CASTG	7 cut(s)	59, 402, 572, 1128, 1189, 2701, 2890
TseFI	GTSAC	6 cut(s)	430, 1271, 1627, 2494, 2944, 2987
Tsp45I	GTSAC	6 cut(s)	430, 1271, 1627, 2494, 2944, 2987
TspGWI	ACGGA	5 cut(s)	9, 701, 1542, 2245, 2772
TspRI	CASTG	7 cut(s)	59, 402, 572, 1128, 1189, 2701, 2890
Van91I	CCANNNNNTGG	1 cut(s)	2652
VpaK11BI	GGWCC	2 cut(s)	778, 1522
XapI	RAATTY	5 cut(s)	304, 341, 673, 1136, 1511
XceI	RCATGY	3 cut(s)	206, 1963, 3057
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1718, 2627
XhoI	CTCGAG	1 cut(s)	1428
XmaJI	CCTAGG	1 cut(s)	3076
XmnI	GAANNNNTTC	6 cut(s)	316, 833, 854, 1440, 1582, 1824
XspI	CTAG	9 cut(s)	14, 75, 641, 1043, 1281, 2195, 2249, 3077, 3104
Zsp2I	ATGCAT	1 cut(s)	382

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.