Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Forward (+)

22539142 .. 22555139

Gene Structure 15998 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1211500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5775 bp

ATGTCAAACCTGGATCTGCCTGGCCCTAGCGGCCCTCAGGGGCTGACGAGACGGCCGTCGAGGAGCGCGGCCACCACTACTTTCTCCACGGAGGTCTTCGACAATGAGGTCGTCCCCTCCGCTCTCGCCTCCATCGCTCCCATCCTCCGTGTCGCCAGCGAGATCGAGGCCGACCGTCCTCGCGTCGCCTATCTCTGTCGGTTCTATGCGTTCGAGAAGGCGCACCGATTGGATCCGAGCTCCAGCGGCCGTGGCGTCCGCCAGTTCAAGACGTTGCTCCTCCAACGACTCGAGAGGGACAATGCATCAAGTTTGGCTTCTCGGGTAAAAAAGACAGATGCCAGAGAAATTGAAAGCTTCTATCAGCAGTATTATGAACATTATGTCAGAGCTCTTGACCAGGGTGAGCAGGCTGACAGAGCCCAACTGGGCAAAGCCTACCAGTCTGCCGGAGTCCTTTTTGAAGTGCTTTGCGCTGTTAACAAGACAGAGAAGGTTGATGAAGTTGCTCCTGAGATTATTGCGGCTGCTAAAGATGTCCAAGAAAAGACGGAAATTTATGCTCCTTATAACATTCTTCCTTTGGATTCTGCTGGTGCTACTCAGTCTATCATGCAACTTGAAGAGGTTAAGGCTGCAGTGGGTGCACTGTGGAACACTCGTGGTTTGAACTGGCCTAGTGCATTCGAGAACCGGCAGAAAGCTGGCGATCTTGACCTCCTTGATTGGCTGAGGGCCATGTTTGGATTCCAGAAAGACAACGTGAGGAACCAGAGGGAGCATTTGATATTGCTACTTGCAAATACTCATATAAGGCTTCATCCCAAACCCGAACCTCTTAATAAGCTGGATGATCGAGCTGTTGATGCAGTTATGGGAAAACTTTTTAAGAACTACAAAACGTGGTGCAAATATTTGGGAAGAAAACATAGTTTGAGACTTCCCCAAGGTCAGCAAGAAATACAGCAAAGGAAGATACTATACATGGGTTTATATCTTCTCATCTGGGGTGAAGCAGCGAACGTCCGCTTTATGCCAGAATGTTTGTGCTATATTTTTCATAATATGGCATATGAACTCCATGGTCTGTTGGCTGGAAATGTCAGCATTGTTACTGGGGAAAATATAAAGCCTTCTTATGGTGGGGATGATGAGGCTTTTTTACGGAAAGTTATAACCCCCTTATATCGTGTAATTGAAAAGGAAGCCAAGAAGGGTGAAAATGGAAAAGCTCCTCACATATCTTGGTGCAACTATGACGATCTCAATGAATATTTCTGGTTTGTGTCGTCTGATTGCTTCTCTCTTGGTTGGCCCATGCGTGATGATGGTGATTTTTTCAAATCCACGCGTGACTTGGTGCAGGGGAGAAAGGGCTCTAGAAGAAAATCTGGAAGCACAGGAAAATCTTATTTTATTGAAACTCGGACATTTTGGCACATTTTTCGAAGTTTTGACCGGTTTTGGACCTTTTACGTACTGGCTCTACAGGCTCTGCTCATTATTGCTTTTAGAGGGATTTCACCATTGGATATTTTTCAAAAGGATGTCCTAAGAGATCTATCAAGTATTTTCATCACAGCGGCATTTCTTCGAGTCCTTCAAAGCATTTTGGATATTGTTTTAAACTTCCCGGGATATCATAGGTGGAGGTTCACTGATGTACTGCGAAATATTCTCAAGATAATTGTTAGTCTTGCATGGGCAATCGTTCTTCCACTGTTTTATGTGCATTCCTTCAATAATGCCCCTAAACAAATCATGGATGTACTGTCATTCCTTAAGAATGTGAATGGTGTTCCTCCTTTATACATCATGGCTGTTGCAGTATATTTGCTGCCAAATTTACTAGCGGCATTTTTGTTCCTTTTCCCGCTGTTCCGACGATGGATTGAAAACTCAGACTGGCATATCATTAGGTTCCTCCTGTGGTGGTCACAGCCAAGAATCTATGTCGGGAGGGGAATGCATGAAAGTCAATTTGCCCTTATTAAGTATACTATTTTTTGGGTGCTGCTTTTGGCTTGCAAGTTTACAGTTAGCTATCTTATCCAGATAAAACCTTTGGTGAAGCCAACTAGAGACATTATGAACATTCGTCGTGTAGATTATCAATGGCATGAATTTTTCCCTAATGCTCAAAACAACTATGGAGCAGTTGTGTCACTTTGGGCACCAGTAATCTTGGTTTATTTGATGGACACTCAAATATGGTATGCTATCTTTCAGACTTTGTATGGTGGTGTTGTTGGGGCATTTGATCGCCTAGGAGAGATTCGAACACTTGGTATGCTGAGATCACGGTTCCAGTCTCTACCTGGTGCATTCAACACATACCTAGTGCCTTCAGATAAGTCAACAAAAAGGGGATTCTCCTTCTCAAAGCGTTTTGTGGAGATTACAGCAAGCAGGAGAAGTGAAGCTGCAAAGTTTGCTCAGATGTGGAATGAAGTGATATGTAGCTTCCGTGAGGAAGATATCATAAATGACAGGGAAATGGATTTGCTGCTAGTTCCTTATTCATCAGATCCCAGCCTGAAGATAATTCAGTGGCCACCCTTCTTGCTTGCTAGCAAAATCCCAATAGCATTAGATATGGCAGTTCAATTTAAATCGAAGGACTCCGATCTCTGGAAGCGGATATGTGCAGATGAATATATGAAATGTGCTGTGATTGAATGTTATGAATCTTTCAAACATGTCCTTAGTACTCTGGTAGTTGGAGAAAATGAGAAAAGGATAATTGGCATCATCGTGAAGGAAATTGAAAGTAACATTTCAAAGAATACATTTCTTGTAAATTTTAGAATGGGTTCTTTGCCCACTCTTTGCAATAAATTTGTAGAGCTTGTGAGGATCTTGAAAGATGGTGATTCATCCAAGCGATCTTCAGTGGTGTTGTTGCTTCAAGATATGCTGGAAGTAGTCACTCGGGATATGATGGTGAATGAGATCCGAGAGTTGGTTGAAGTTGGTCATAGTAGCAAGGATGCTGGAAGGCAACTTTTTGCTGGAACTGATGCAAAACCTGCAATATTATTCCCTCCTCCAGTTACAGCACGGTGGGAAGAGCAGATAAGCCGCCTCCATCTTCTGCTAACAGTTAAGGAATCTGCCATTGACGTACCAACAAACCTAGAAGCGCGTAGAAGGATTGCATTTTTTACAAATTCATTGTTCATGGATATGCCGCGTGCTCCCCGAGTCCGTAAAATGCTTTCATTCAGCATCATGACCCCATACTATAGTGAGGAGACTGTGTATTCCAAATCAGACCTTGAGATGGAAAATGAAGATGGTGTATCAATCATATACTACTTGCAGAAGATTTTCCCAGATGAATGGAATAACTTCATGGAGCGCCTTAATTGTAAAAAGGATAGTGAAATATGGGAGAATGAAGAAAACATTTTGCATCTTCGTCATTGGGTCTCCTTACGAGGACAAACTCTCTGCAGGACTGTTAGAGGAATGATGTACTACCGACGAGCACTGAAGCTTCAGGCTTTTCTTGACATGGCTACTGAAACCGATATACTAGATGGCTACAAAGCAATCATGGTTCCACCAGAAGAAGAAAAGAAAAGCCAGAGATCCCTTTATGCTCAATTAGAAGCAGTGGCTGACCTTAAATTCACCTATGTTGCTACCTGCCAAAACTATGGCAATCAGAAACGCAGTGGAGATCGACGTGCAACAGACATCTTGAATCTGATGGTTAATAATCCCTCTCTTCGGGTTGCATATATCGATGAAGTTGAAGAGAGTGATGCTGGTAGTGGAAAGGTGCAAAAGGTTTACTACTCTGTCTTGGTCAAAGCTGTTGACAATCATGACCAGGAAATATATCGTATAAAATTGCCTGGTTCGGCAAAGATTGGAGAAGGAAAACCTGAAAACCAGAACCATGCTATAATTTTTACTCGAGGTGAAGCTCTTCAGGCCATTGACATGAACCAGGACAATTACTTAGAAGAAGCTTTTAAAATGCGAAACCTTCTTGAAGAATTTAATGAGGACCATGGAGTGCGTCCACCTTCAATTTTAGGTGTTCGTGAGCATATCTTTACTGGAAGTGTTTCTTCATTGGCCTGGTTTATGTCAAATCAAGAAATGAGCTTTGTGACCATAGGTCAAAGAGTTCTTGCAAGACCTTTGAAGATTCGCTTCCACTATGGACACCCAGATGTCTTTGATAGAATCTTCCACATTACTCGTGGTGGCATGAGCAAGGCTTCCCGTGGCATCAATCTTAGCGAGGACATCTTTGCTGGTTTTAACTCAACGTTAAGGAGAGGGAACATTACGCATCACGAATATATTCAAGTTGGGAAGGGTAGGGATGTTGGGCTCAACCAAATCTCTCTCTTTGAAGCTAAAGTTGCTTGTGGTAATGGAGAGCAGACGCTTAGCAGGGATATCTACCGCTTGGGGCACCGTTTTGACTTCTTTCGCATGTTGTCCTGTTATTTTTCGACAATAGGGTTTTATATCAGCGCAATGTTGGTTGTCCTTACAGTCTATGCTTTCTTGTACGGAAGACTTTACTTGTCATTAAGTGGAATGGAGAAGACAATTGTGAACTATGCCGCCACCAGGGGAAATAATGTTCTACAAACAGCCATGGCTTCACAATCTGTTTTCCAATTAGGTCTTTTAACTTCCTTGCCCATGATCATGGAAATTGGACTAGAAAGAGGGTTCAGAACTGCATTAGGGGACATGATAATCATGCAGCTGCAACTAGCATCTGTTTTCTTCACCTTCTCTCTTGGTACAAAAGTTCATTATTTTGGCCGAACAGTGCTGCACGGTGGGGCTAAATACAGAGCAACTGGCCGTGGCTTTGTTGTCCGGCATGAAAAGTTTGCAGAGAACTACAGGATGTACTCAAGGAGTCACTTTGTGAAAGGCCTCGAACTTATGGTACTGCTTATAGTTTATCAGATATATGGTTCAGCAGTAAAAGGCACAATATCATATATCTTTGTCACATTCTCCATGTGGTTCTTAGTGGTTTCGTGGCTGTTCGCTCCCTTCCTGTTTAACCCTTCGGGATTTGAATGGCAAAAGATAGTCGAAGATTGGGATGATTGGGCAAAATGGATAAGTAGCCCTGGTGGTATTGGTGTGCCAGCAACCAAGAGCTGGGAGTCTTGGTGGGATGAGGAACAAGAACACCTCCACCACACAGGGGTTTTGGGACGATTTTGGGAGATTGTTCTTGCTCTGCGCTTCTTTCTCTTCCAATACGGAATTGTGTACCATCTAAATGTGGCCAGGGGTGATAAAAGCATCATGGTTTATGGGCTGTCATGGCTGGTCATTGTCGCTGTGATGATCATCCTAAAGGTTGTGTCAATGGGAAGACAGCGGTTCAGTGCAGATTTCCAGCTGATGTTCAGGCTTCTCAAGTTGTTCCTGTTTATTGGGTTTGTTGTCACTATTGGAATGCTTTTCGCTTTCCTCAGTCTTACTGTTGGCGACATCTTTGTGAGCTTATTGGCCTTCTTGCCTACTGGATGGGCGCTACTACTGATGTCACAAGCGTGCAAGCCATTGGTGAAGGTCCTTGGAATGTGGGGATCTGTCAAAGCTCTAGCAAGAGGGTATGAGTACGTGATGGGGCTCGTCATCTTCGCACCAGTAGCTGTTCTTGCATGGTTCCCATTTGTCTCAGAGTTCCAGACCAGGCTGCTATTCAACCAAGCTTTCAGCCGAGGGCTCCAGATCCAGCGTATTCTTGCTGGTGGGAAGAAGCACAAGTCAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1925

Amino Acids

220.82

Weight (kDa)

9.08

Isoelectric Point (pI)

43.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vta1	PF04652	44 - 176	2.7e-13	Vta1 like
FKS1_dom1	PF14288	325 - 438	2.5e-39	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	852 - 1035	3.5e-67	Callose synthase 1 domain
Glucan_synthase	PF02364	1042 - 1736	3.3e-232	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	570, 1175
AasI	GACNNNNNNGTC	1 cut(s)	107
Acc36I	ACCTGC	2 cut(s)	3037, 3659
AccB1I	GGYRCC	2 cut(s)	2173, 4434
AccB7I	CCANNNNNTGG	1 cut(s)	5148
AccBSI	CCGCTC	1 cut(s)	122
AccI	GTMKAC	1 cut(s)	1997
AccII	CGCG	5 cut(s)	68, 183, 1351, 3145, 3193
AclI	AACGTT	1 cut(s)	4286
AclWI	GGATC	9 cut(s)	21, 227, 240, 2522, 2864, 2947, 3588, 5593, 5725
AcoI	YGGCCR	7 cut(s)	53, 69, 247, 2552, 4795, 4837, 5277
AcsI	RAATTY	8 cut(s)	555, 1843, 2123, 2800, 2837, 3169, 3632, 4007
AcuI	CTGAAG	6 cut(s)	2331, 2558, 2874, 3485, 3515, 3923
AcyI	GRCGYC	1 cut(s)	255
AdeI	CACNNNGTG	1 cut(s)	4906
AfiI	CCNNNNNNNGG	8 cut(s)	1139, 2631, 2962, 3283, 3735, 4596, 5148, 5194
AflII	CTTAAG	1 cut(s)	1781
AflIII	ACRYGT	2 cut(s)	1349, 2698
AgeI	ACCGGT	1 cut(s)	1458
AhdI	GACNNNNNGTC	1 cut(s)	4131
AjiI	CACGTC	1 cut(s)	3692
AleI	CACNNNNGTG	1 cut(s)	5548
AloI	GAACNNNNNNTCC	2 cut(s)	4593, 4625
Alw21I	GWGCWC	5 cut(s)	242, 394, 649, 3199, 3493
Alw26I	GTCTC	7 cut(s)	43, 931, 2076, 2316, 3248, 3436, 5680
Alw44I	GTGCAC	1 cut(s)	645
AlwI	GGATC	9 cut(s)	21, 227, 240, 2522, 2864, 2947, 3588, 5593, 5725
AlwNI	CAGNNNCTG	4 cut(s)	43, 1495, 3623, 5651
Ama87I	CYCGRG	6 cut(s)	290, 321, 1633, 2931, 3201, 3924
ApaLI	GTGCAC	1 cut(s)	645
ApoI	RAATTY	8 cut(s)	555, 1843, 2123, 2800, 2837, 3169, 3632, 4007
AsiGI	ACCGGT	1 cut(s)	1458
Asp700I	GAANNNNTTC	6 cut(s)	2812, 3329, 3350, 3936, 4078, 4320
AspA2I	CCTAGG	1 cut(s)	2266
AspLEI	GCGC	8 cut(s)	68, 223, 476, 3145, 3363, 4499, 5235, 5530
AspS9I	GGNCC	7 cut(s)	23, 32, 735, 1314, 1467, 4018, 5569
AsuC2I	CCSGG	2 cut(s)	1634, 1635
AsuII	TTCGAA	2 cut(s)	1447, 2278
AsuNHI	GCTAGC	1 cut(s)	2570
AvaI	CYCGRG	6 cut(s)	290, 321, 1633, 2931, 3201, 3924
AvaII	GGWCC	3 cut(s)	1467, 4018, 5569
AvrII	CCTAGG	1 cut(s)	2266
AxyI	CCTNAGG	1 cut(s)	36
BaeGI	GKGCMC	3 cut(s)	649, 2176, 4437
BaeI	ACNNNNGTAYC	2 cut(s)	4767, 4800
BalI	TGGCCA	2 cut(s)	2554, 5279
BamHI	GGATCC	1 cut(s)	232
BanI	GGYRCC	2 cut(s)	2173, 4434
BanII	GRGCYC	7 cut(s)	242, 394, 424, 1379, 4353, 5631, 5727
BarI	GAAGNNNNNNTAC	2 cut(s)	965, 997
BauI	CACGAG	2 cut(s)	660, 4215
BbsI	GAAGAC	4 cut(s)	88, 4546, 4577, 5374
Bbv12I	GWGCWC	5 cut(s)	242, 394, 649, 3199, 3493
BbvCI	CCTCAGC	1 cut(s)	731
BceAI	ACGGC	4 cut(s)	40, 68, 234, 4824
BcgI	CGANNNNNNTGC	2 cut(s)	1427, 1461
BclI	TGATCA	2 cut(s)	4674, 5340
BcnI	CCSGG	2 cut(s)	1634, 1635
BcoDI	GTCTC	7 cut(s)	43, 931, 2076, 2316, 3248, 3436, 5680
BfmI	CTRYAG	5 cut(s)	636, 1487, 3244, 3454, 4879
BfoI	RGCGCY	2 cut(s)	3364, 5531
BfrI	CTTAAG	1 cut(s)	1781
BfuAI	ACCTGC	2 cut(s)	3037, 3659
BglI	GCCNNNNNGGC	1 cut(s)	30
BglII	AGATCT	1 cut(s)	1558
BlnI	CCTAGG	1 cut(s)	2266
BlpI	GCTNAGC	1 cut(s)	4409
BmcAI	AGTACT	1 cut(s)	2710
Bme18I	GGWCC	3 cut(s)	1467, 4018, 5569
BmeRI	GACNNNNNGTC	1 cut(s)	4131
BmeT110I	CYCGRG	6 cut(s)	290, 321, 1633, 2931, 3201, 3924
BmgBI	CACGTC	1 cut(s)	3692
BmgT120I	GGNCC	7 cut(s)	23, 32, 735, 1314, 1467, 4018, 5569
BmiI	GGNNCC	9 cut(s)	234, 770, 1922, 2175, 2306, 3564, 4436, 5666, 5726
BmrI	ACTGGG	2 cut(s)	437, 1125
BmtI	GCTAGC	1 cut(s)	2574
BmuI	ACTGGG	2 cut(s)	437, 1125
BoxI	GACNNNNGTC	1 cut(s)	55
BpiI	GAAGAC	4 cut(s)	88, 4546, 4577, 5374
BpmI	CTGGAG	3 cut(s)	226, 3033, 5711
Bpu10I	CCTNAGC	1 cut(s)	731
Bpu1102I	GCTNAGC	1 cut(s)	4409
Bpu14I	TTCGAA	2 cut(s)	1447, 2278
BpuEI	CTTGAG	4 cut(s)	1664, 3299, 4876, 5396
BpuMI	CCSGG	2 cut(s)	1634, 1635
Bsa29I	ATCGAT	1 cut(s)	3750
BsaAI	YACGTR	2 cut(s)	1477, 5620
BsaBI	GATNNNNATC	1 cut(s)	5342
BsaHI	GRCGYC	1 cut(s)	255
BsaI	GGTCTC	1 cut(s)	3436
BsaWI	WCCGGW	1 cut(s)	1458
BsaXI	ACNNNNNCTCC	6 cut(s)	2145, 2175, 3245, 3275, 5144, 5174
Bsc4I	CCNNNNNNNGG	8 cut(s)	1139, 2631, 2962, 3283, 3735, 4596, 5148, 5194
Bse118I	RCCGGY	2 cut(s)	693, 1458
Bse21I	CCTNAGG	1 cut(s)	36
Bse3DI	GCAATG	1 cut(s)	4506
Bse8I	GATNNNNATC	1 cut(s)	5342
BseCI	ATCGAT	1 cut(s)	3750
BseJI	GATNNNNATC	1 cut(s)	5342
BseLI	CCNNNNNNNGG	8 cut(s)	1139, 2631, 2962, 3283, 3735, 4596, 5148, 5194
BseMI	GCAATG	1 cut(s)	4506
BseMII	CTCAG	9 cut(s)	50, 504, 617, 722, 1914, 2285, 2450, 5482, 5691
BseRI	GAGGAG	5 cut(s)	76, 269, 1224, 3036, 3266
BseSI	GKGCMC	3 cut(s)	649, 2176, 4437
BseX3I	CGGCCG	2 cut(s)	53, 247
BseYI	CCCAGC	2 cut(s)	2531, 5148
BsgI	GTGCAG	4 cut(s)	1382, 2667, 4793, 5403
Bsh1236I	CGCG	5 cut(s)	68, 183, 1351, 3145, 3193
Bsh1285I	CGRYCG	3 cut(s)	56, 175, 250
BshNI	GGYRCC	2 cut(s)	2173, 4434
BshTI	ACCGGT	1 cut(s)	1458
BshVI	ATCGAT	1 cut(s)	3750
BsiEI	CGRYCG	3 cut(s)	56, 175, 250
BsiHKAI	GWGCWC	5 cut(s)	242, 394, 649, 3199, 3493
BsiHKCI	CYCGRG	6 cut(s)	290, 321, 1633, 2931, 3201, 3924
BsiSI	CCGG	5 cut(s)	450, 694, 1459, 1634, 4855
BslFI	GGGAC	4 cut(s)	98, 311, 4733, 5217
BslI	CCNNNNNNNGG	8 cut(s)	1139, 2631, 2962, 3283, 3735, 4596, 5148, 5194
BsmAI	GTCTC	7 cut(s)	43, 931, 2076, 2316, 3248, 3436, 5680
BsmBI	CGTCTC	1 cut(s)	43
BsmFI	GGGAC	4 cut(s)	98, 311, 4733, 5217
BsmI	GAATGC	5 cut(s)	683, 1732, 1971, 2324, 5457
Bso31I	GGTCTC	1 cut(s)	3436
BsoBI	CYCGRG	6 cut(s)	290, 321, 1633, 2931, 3201, 3924
Bsp119I	TTCGAA	2 cut(s)	1447, 2278
Bsp1720I	GCTNAGC	1 cut(s)	4409
Bsp19I	CCATGG	3 cut(s)	1081, 4021, 4623
BspCNI	CTCAG	9 cut(s)	49, 505, 616, 723, 1913, 2286, 2449, 5481, 5690
BspDI	ATCGAT	1 cut(s)	3750
BspFNI	CGCG	5 cut(s)	68, 183, 1351, 3145, 3193
BspHI	TCATGA	2 cut(s)	3231, 3832
BspLI	GGNNCC	9 cut(s)	234, 770, 1922, 2175, 2306, 3564, 4436, 5666, 5726
BspMAI	CTGCAG	2 cut(s)	640, 3458
BspMI	ACCTGC	2 cut(s)	3037, 3659
BspOI	GCTAGC	1 cut(s)	2574
BspPI	GGATC	9 cut(s)	21, 227, 240, 2522, 2864, 2947, 3588, 5593, 5725
BspQI	GCTCTTC	2 cut(s)	3063, 3942
BspT104I	TTCGAA	2 cut(s)	1447, 2278
BspT107I	GGYRCC	2 cut(s)	2173, 4434
BspTI	CTTAAG	1 cut(s)	1781
BspTNI	GGTCTC	1 cut(s)	3436
BsrBI	CCGCTC	1 cut(s)	122
BsrDI	GCAATG	1 cut(s)	4506
BsrFI	RCCGGY	2 cut(s)	693, 1458
BssAI	RCCGGY	2 cut(s)	693, 1458
BssNAI	GTATAC	1 cut(s)	1998
BssNI	GRCGYC	1 cut(s)	255
BssSI	CACGAG	2 cut(s)	660, 4215
BssT1I	CCWWGG	6 cut(s)	946, 1081, 2266, 4021, 4623, 5572
Bst1107I	GTATAC	1 cut(s)	1998
Bst2BI	CACGAG	2 cut(s)	660, 4215
Bst6I	CTCTTC	6 cut(s)	618, 3063, 3738, 3756, 3942, 5249
BstACI	GRCGYC	1 cut(s)	255
BstAFI	CTTAAG	1 cut(s)	1781
BstAPI	GCANNNNNTGC	3 cut(s)	644, 2432, 3029
BstBAI	YACGTR	2 cut(s)	1477, 5620
BstBI	TTCGAA	2 cut(s)	1447, 2278
BstDSI	CCRYGG	7 cut(s)	87, 250, 1081, 4021, 4240, 4623, 4840
BstFNI	CGCG	5 cut(s)	68, 183, 1351, 3145, 3193
BstH2I	RGCGCY	2 cut(s)	3364, 5531
BstHHI	GCGC	8 cut(s)	68, 223, 476, 3145, 3363, 4499, 5235, 5530
BstMAI	GTCTC	7 cut(s)	43, 931, 2076, 2316, 3248, 3436, 5680
BstMCI	CGRYCG	3 cut(s)	56, 175, 250
BstNSI	RCATGY	2 cut(s)	2702, 4459
BstPAI	GACNNNNGTC	1 cut(s)	55
BstSFI	CTRYAG	5 cut(s)	636, 1487, 3244, 3454, 4879
BstSLI	GKGCMC	3 cut(s)	649, 2176, 4437
BstSNI	TACGTA	1 cut(s)	1477
BstUI	CGCG	5 cut(s)	68, 183, 1351, 3145, 3193
BstV2I	GAAGAC	4 cut(s)	88, 4546, 4577, 5374
BstX2I	RGATCY	9 cut(s)	13, 232, 1558, 2527, 2856, 2952, 3593, 5585, 5730
BstXI	CCANNNNNNTGG	3 cut(s)	3342, 3662, 4678
BstYI	RGATCY	9 cut(s)	13, 232, 1558, 2527, 2856, 2952, 3593, 5585, 5730
BstZ17I	GTATAC	1 cut(s)	1998
BstZI	CGGCCG	2 cut(s)	53, 247
Bsu15I	ATCGAT	1 cut(s)	3750
Bsu36I	CCTNAGG	1 cut(s)	36
BsuTUI	ATCGAT	1 cut(s)	3750
BtgI	CCRYGG	7 cut(s)	87, 250, 1081, 4021, 4240, 4623, 4840
BtgZI	GCGATG	1 cut(s)	118
BtrI	CACGTC	1 cut(s)	3692
BtsI	GCAGTG	3 cut(s)	645, 3624, 3685
BveI	ACCTGC	2 cut(s)	3037, 3659
CaiI	CAGNNNCTG	4 cut(s)	43, 1495, 3623, 5651
CciI	TCATGA	2 cut(s)	3231, 3832
CfoI	GCGC	8 cut(s)	68, 223, 476, 3145, 3363, 4499, 5235, 5530
Cfr10I	RCCGGY	2 cut(s)	693, 1458
Cfr13I	GGNCC	7 cut(s)	23, 32, 735, 1314, 1467, 4018, 5569
Cfr9I	CCCGGG	1 cut(s)	1633
ClaI	ATCGAT	1 cut(s)	3750
CseI	GACGC	4 cut(s)	172, 244, 4019, 4414
CsiI	ACCWGGT	1 cut(s)	2317
CspAI	ACCGGT	1 cut(s)	1458
CspCI	CAANNNNNGTGG	2 cut(s)	2544, 2579
DraI	TTTAAA	3 cut(s)	1626, 2611, 3985
DraIII	CACNNNGTG	1 cut(s)	4906
DrdI	GACNNNNNNGTC	1 cut(s)	107
DriI	GACNNNNNGTC	1 cut(s)	4131
DseDI	GACNNNNNNGTC	1 cut(s)	107
EaeI	YGGCCR	7 cut(s)	53, 69, 247, 2552, 4795, 4837, 5277
EagI	CGGCCG	2 cut(s)	53, 247
Eam1104I	CTCTTC	6 cut(s)	618, 3063, 3738, 3756, 3942, 5249
Eam1105I	GACNNNNNGTC	1 cut(s)	4131
EarI	CTCTTC	6 cut(s)	618, 3063, 3738, 3756, 3942, 5249
EciI	GGCGGA	1 cut(s)	248
Ecl136II	GAGCTC	2 cut(s)	240, 392
EclXI	CGGCCG	2 cut(s)	53, 247
Eco105I	TACGTA	1 cut(s)	1477
Eco130I	CCWWGG	6 cut(s)	946, 1081, 2266, 4021, 4623, 5572
Eco147I	AGGCCT	1 cut(s)	4914
Eco24I	GRGCYC	7 cut(s)	242, 394, 424, 1379, 4353, 5631, 5727
Eco31I	GGTCTC	1 cut(s)	3436
Eco32I	GATATC	3 cut(s)	1640, 2479, 4420
Eco47I	GGWCC	3 cut(s)	1467, 4018, 5569
Eco52I	CGGCCG	2 cut(s)	53, 247
Eco53kI	GAGCTC	2 cut(s)	240, 392
Eco57I	CTGAAG	6 cut(s)	2331, 2558, 2874, 3485, 3515, 3923
Eco81I	CCTNAGG	1 cut(s)	36
Eco88I	CYCGRG	6 cut(s)	290, 321, 1633, 2931, 3201, 3924
EcoICRI	GAGCTC	2 cut(s)	240, 392
EcoO109I	RGGNCCY	1 cut(s)	5569
EcoRV	GATATC	3 cut(s)	1640, 2479, 4420
EcoT14I	CCWWGG	6 cut(s)	946, 1081, 2266, 4021, 4623, 5572
EcoT22I	ATGCAT	2 cut(s)	307, 1971
EcoT38I	GRGCYC	7 cut(s)	242, 394, 424, 1379, 4353, 5631, 5727
ErhI	CCWWGG	6 cut(s)	946, 1081, 2266, 4021, 4623, 5572
Esp3I	CGTCTC	1 cut(s)	43
FalI	AAGNNNNNCTT	6 cut(s)	301, 333, 4066, 4098, 4151, 4183
FaqI	GGGAC	4 cut(s)	98, 311, 4733, 5217
FauI	CCCGC	1 cut(s)	1881
FauNDI	CATATG	1 cut(s)	1072
FbaI	TGATCA	2 cut(s)	4674, 5340
FblI	GTMKAC	1 cut(s)	1997
FriOI	GRGCYC	7 cut(s)	242, 394, 424, 1379, 4353, 5631, 5727
GlaI	GCGC	8 cut(s)	67, 222, 475, 3144, 3362, 4498, 5234, 5529
GsaI	CCCAGC	2 cut(s)	2535, 5152
GsuI	CTGGAG	3 cut(s)	226, 3033, 5711
HaeII	RGCGCY	2 cut(s)	3364, 5531
HapII	CCGG	5 cut(s)	450, 694, 1459, 1634, 4855
HgaI	GACGC	4 cut(s)	172, 244, 4019, 4414
HhaI	GCGC	8 cut(s)	68, 223, 476, 3145, 3363, 4499, 5235, 5530
Hin1I	GRCGYC	1 cut(s)	255
Hin6I	GCGC	8 cut(s)	66, 221, 474, 3143, 3361, 4497, 5233, 5528
HinP1I	GCGC	8 cut(s)	66, 221, 474, 3143, 3361, 4497, 5233, 5528
HincII	GTYRAC	3 cut(s)	481, 2358, 3826
HindII	GTYRAC	3 cut(s)	481, 2358, 3826
HindIII	AAGCTT	4 cut(s)	355, 3497, 3978, 5709
HpaI	GTTAAC	1 cut(s)	481
HpaII	CCGG	5 cut(s)	450, 694, 1459, 1634, 4855
Hpy99I	CGWCG	6 cut(s)	61, 188, 1887, 2103, 3489, 3693
HpyCH4IV	ACGT	9 cut(s)	272, 762, 902, 1023, 1476, 3123, 3691, 4286, 5619
HpySE526I	ACGT	9 cut(s)	272, 762, 902, 1023, 1476, 3123, 3691, 4286, 5619
Hsp92I	GRCGYC	1 cut(s)	255
HspAI	GCGC	8 cut(s)	66, 221, 474, 3143, 3361, 4497, 5233, 5528
Ksp22I	TGATCA	2 cut(s)	4674, 5340
KspAI	GTTAAC	1 cut(s)	481
LguI	GCTCTTC	2 cut(s)	3063, 3942
MabI	ACCWGGT	1 cut(s)	2317
MaeII	ACGT	9 cut(s)	272, 762, 902, 1023, 1476, 3123, 3691, 4286, 5619
MbiI	CCGCTC	1 cut(s)	122
MfeI	CAATTG	1 cut(s)	4575
MflI	RGATCY	9 cut(s)	13, 232, 1558, 2527, 2856, 2952, 3593, 5585, 5730
MlsI	TGGCCA	2 cut(s)	2554, 5279
MluI	ACGCGT	1 cut(s)	1349
MluNI	TGGCCA	2 cut(s)	2554, 5279
MlyI	GAGTC	7 cut(s)	282, 462, 1605, 2615, 3213, 4906, 5162
MmeI	TCCRAC	3 cut(s)	307, 1906, 2701
Mox20I	TGGCCA	2 cut(s)	2554, 5279
Mph1103I	ATGCAT	2 cut(s)	307, 1971
MroXI	GAANNNNTTC	6 cut(s)	2812, 3329, 3350, 3936, 4078, 4320
MscI	TGGCCA	2 cut(s)	2554, 5279
MslI	CAYNNNNRTG	8 cut(s)	2753, 3185, 3950, 4185, 4676, 5271, 5492, 5548
Msp20I	TGGCCA	2 cut(s)	2554, 5279
MspA1I	CMGCKG	6 cut(s)	246, 1583, 1876, 4738, 5374, 5395
MspCI	CTTAAG	1 cut(s)	1781
MspI	CCGG	5 cut(s)	450, 694, 1459, 1634, 4855
MunI	CAATTG	1 cut(s)	4575
Mva1269I	GAATGC	5 cut(s)	683, 1732, 1971, 2324, 5457
MvnI	CGCG	5 cut(s)	68, 183, 1351, 3145, 3193
NciI	CCSGG	2 cut(s)	1634, 1635
NcoI	CCATGG	3 cut(s)	1081, 4021, 4623
NdeI	CATATG	1 cut(s)	1072
NheI	GCTAGC	1 cut(s)	2570
NlaIV	GGNNCC	9 cut(s)	234, 770, 1922, 2175, 2306, 3564, 4436, 5666, 5726
NmeAIII	GCCGAG	1 cut(s)	5744
NmuCI	GTSAC	9 cut(s)	1352, 1935, 2163, 2926, 4123, 4898, 4990, 5440, 5541
NsiI	ATGCAT	2 cut(s)	307, 1971
NspI	RCATGY	2 cut(s)	2702, 4459
NspV	TTCGAA	2 cut(s)	1447, 2278
OliI	CACNNNNGTG	1 cut(s)	5548
PaeR7I	CTCGAG	2 cut(s)	290, 3924
PagI	TCATGA	2 cut(s)	3231, 3832
PceI	AGGCCT	1 cut(s)	4914
PciI	ACATGT	1 cut(s)	2698
PciSI	GCTCTTC	2 cut(s)	3063, 3942
PcsI	WCGNNNNNNNCGW	1 cut(s)	53
PctI	GAATGC	5 cut(s)	683, 1732, 1971, 2324, 5457
PdmI	GAANNNNTTC	6 cut(s)	2812, 3329, 3350, 3936, 4078, 4320
PflMI	CCANNNNNTGG	1 cut(s)	5148
PinAI	ACCGGT	1 cut(s)	1458
PleI	GAGTC	7 cut(s)	282, 461, 1604, 2615, 3212, 4905, 5161
PpsI	GAGTC	7 cut(s)	282, 461, 1604, 2615, 3212, 4905, 5161
Ppu21I	YACGTR	2 cut(s)	1477, 5620
PpuMI	RGGWCCY	1 cut(s)	5569
PscI	ACATGT	1 cut(s)	2698
PshAI	GACNNNNGTC	1 cut(s)	55
PsiI	TTATAA	2 cut(s)	570, 1175
Psp124BI	GAGCTC	2 cut(s)	242, 394
Psp1406I	AACGTT	1 cut(s)	4286
Psp5II	RGGWCCY	1 cut(s)	5569
PspFI	CCCAGC	2 cut(s)	2531, 5148
PspN4I	GGNNCC	9 cut(s)	234, 770, 1922, 2175, 2306, 3564, 4436, 5666, 5726
PspPI	GGNCC	7 cut(s)	23, 32, 735, 1314, 1467, 4018, 5569
PspPPI	RGGWCCY	1 cut(s)	5569
PspXI	VCTCGAGB	1 cut(s)	3924
PsrI	GAACNNNNNNTAC	2 cut(s)	4911, 4943
PstI	CTGCAG	2 cut(s)	640, 3458
PstNI	CAGNNNCTG	4 cut(s)	43, 1495, 3623, 5651
PsuI	RGATCY	9 cut(s)	13, 232, 1558, 2527, 2856, 2952, 3593, 5585, 5730
PvuII	CAGCTG	2 cut(s)	4738, 5395
RseI	CAYNNNNRTG	8 cut(s)	2753, 3185, 3950, 4185, 4676, 5271, 5492, 5548
SacI	GAGCTC	2 cut(s)	242, 394
SapI	GCTCTTC	2 cut(s)	3063, 3942
Sau96I	GGNCC	7 cut(s)	23, 32, 735, 1314, 1467, 4018, 5569
ScaI	AGTACT	1 cut(s)	2710
SchI	GAGTC	7 cut(s)	282, 462, 1605, 2615, 3213, 4906, 5162
SexAI	ACCWGGT	1 cut(s)	2317
SfcI	CTRYAG	5 cut(s)	636, 1487, 3244, 3454, 4879
SfiI	GGCCNNNNNGGCC	1 cut(s)	30
Sfr274I	CTCGAG	2 cut(s)	290, 3924
SfuI	TTCGAA	2 cut(s)	1447, 2278
SinI	GGWCC	3 cut(s)	1467, 4018, 5569
SlaI	CTCGAG	2 cut(s)	290, 3924
SmaI	CCCGGG	1 cut(s)	1635
SmiI	ATTTAAAT	1 cut(s)	2611
SmiMI	CAYNNNNRTG	8 cut(s)	2753, 3185, 3950, 4185, 4676, 5271, 5492, 5548
SmlI	CTYRAG	7 cut(s)	290, 1679, 1781, 3278, 3924, 4891, 5411
SmoI	CTYRAG	7 cut(s)	290, 1679, 1781, 3278, 3924, 4891, 5411
SnaBI	TACGTA	1 cut(s)	1477
SseBI	AGGCCT	1 cut(s)	4914
SspI	AATATT	4 cut(s)	914, 1274, 1675, 3036
SstI	GAGCTC	2 cut(s)	242, 394
StuI	AGGCCT	1 cut(s)	4914
StyI	CCWWGG	6 cut(s)	946, 1081, 2266, 4021, 4623, 5572
SwaI	ATTTAAAT	1 cut(s)	2611
TaiI	ACGT	9 cut(s)	275, 765, 905, 1026, 1479, 3126, 3694, 4289, 5622
TatI	WGTACW	5 cut(s)	1663, 1768, 2708, 3477, 4887
TauI	GCSGC	9 cut(s)	33, 71, 249, 527, 1586, 1856, 3084, 3193, 4592
TseFI	GTSAC	9 cut(s)	1352, 1935, 2163, 2926, 4123, 4898, 4990, 5440, 5541
Tsp45I	GTSAC	9 cut(s)	1352, 1935, 2163, 2926, 4123, 4898, 4990, 5440, 5541
TspGWI	ACGGA	8 cut(s)	104, 137, 566, 1180, 2456, 3197, 4551, 5268
TspMI	CCCGGG	1 cut(s)	1633
Van91I	CCANNNNNTGG	1 cut(s)	5148
Vha464I	CTTAAG	1 cut(s)	1781
VneI	GTGCAC	1 cut(s)	645
VpaK11BI	GGWCC	3 cut(s)	1467, 4018, 5569
XapI	RAATTY	8 cut(s)	555, 1843, 2123, 2800, 2837, 3169, 3632, 4007
XbaI	TCTAGA	1 cut(s)	1379
XceI	RCATGY	2 cut(s)	2702, 4459
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1354, 2315, 4214
XhoI	CTCGAG	2 cut(s)	290, 3924
XmaI	CCCGGG	1 cut(s)	1633
XmaJI	CCTAGG	1 cut(s)	2266
XmiI	GTMKAC	1 cut(s)	1997
XmnI	GAANNNNTTC	6 cut(s)	2812, 3329, 3350, 3936, 4078, 4320
ZrmI	AGTACT	1 cut(s)	2710
Zsp2I	ATGCAT	2 cut(s)	307, 1971

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.