Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Reverse (-)

34255216 .. 34262239

Gene Structure 7024 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG299200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGGATTTGCTGCTGGTTCCTTATTCTTCAGATACGAGCCTGAAAATAATTCAGTGGCCACCTTTCTTGCTTGCTAGCAAAATCCCAATAGCATTAGATATGGCAGCTCAATTCAAAAACAAGGACTCTGATCTCTGGAAGCGGATATGTGGAGATGAATATATGAAGTGTGCTGTGATTGAATGTTATGAGTCTTTCAAACATGTCCTTAAGGCTTTGGTGGTTGGAGAAAATGAGAAAAGGATCATTGGCATCATTATTAAGGAAATTGAAAGTAACATTTCCAAGAATACATTTCTTGATAATTTTAGAATGGCCTCTTTACCCACTCTCTGCCAGAAATTTGTAGAGCTTGTGGTGATCTTGAAAGATGGTGATTCATCCAAGCGATCTGCGGTGGTGCTGCTGCTGCAAGATATGTTGGAAGTTGTCACTCGTGATATGATGGTGAATGAGATCCGAGAGGTGGTTGAAGTTGGCCATAGTAGCAAGGACACTGGAAGGCAGCTTTTTGCTGGTACTGATGCAAAACCTGCGATCGTATTCCCTCCTCCAGTTACAGCACAATGGGAAGAACAGATAAGGCGCCTCCATCTGCTGTTGACTGTTAAGGAGTCTGCAATTGATGTACCAATAAACCTTGAAGCACGTAGAAGGATTTCATTCTTTGCAAATTCATTGTTCATGGATATGCCCCGTGCTCCCCGTGTTCGTAAGATGCTTTCGTTCAGCATTTTGACTCCCTACTATAGTGAGGAGACTGTGTATTCCAAAAGTGACCTTGAGATGGAAAATGAAGATGGTGTATCAATCATATATTACTTGCAGAAGATTTTCCCAGATGAATGGACTAACTTCATGGAGCGGCTTAATTGTAAAAAGGATGCTGAAATATGGGAAAATGAAGAGAACATTTTGCAGCTGCGTCATTGGGTCTCCTTACGAGGACAAACTCTCTGCAGGACAGTTAGAGGAATGATGTATTACCGACGAGCTCTGAAGCTTCAGGCTTTTCTTGATATGGCTAATGAAGAAGAGATACTAGAAGGGTACAAAGCCGTCACAGTTTCACCAGAGGACGAAAAGAAGAGCCGGAGATCCCTATTTGCTCAACTAGAAGCAGTGGCTGATCTTAAATTTACCTATGTTGCTACTTGCCAAAACTATGGCAATCAGAAGCGAAGTGGAGATCGTCGTGCAACTGACATCCTGAATCTGATGGTTAATAATCCCTCTCTCAGGGTTGCATATATTGATGAAGTTGAAGAACGAGAAGGTGAAAAGGTGCAAAAGGTTTACTATTCTGTATTGGTCAAAGCAGTTGACAACCTTGACCAGTTGGTTGTGATGACGGTCTACTCTTTCCTGTATGGTAGACTCTACTTGTCATTGAGTGGAATGGAGAAGACAATTATAACCTATGCTGCTAGAAGGGGAAATAATGCTCTACAAACAGCTATGGCTTCACAATCTGTTGTCCAATTAGGTTTGTTGACGTCCTTGCCCATGATCATGGAAATCGGACTTGAAAGAGGGTTCAGAACTGCATTAGGGGACACGATAATAATGCAGCTGCAGCTAGCATCAGTTTTCTTTACTTTCTCACTGGGTACAAAGGTTCATTACTATGGGAGAACAATCCTGCATGGTGGGGCTAAGTACAGAGCAACTGGCCGTGGCTTTGTTGTTCGGCATGAGAGGTTTGGTGAAAACTACAGGATGTACTCACGGAGTCACTTTACAAAAGGCCTGGAGCTTATGATTCTGCTTATTATTTATAGTATATATGGCTCAGCAGCAGATGGCTCAGCTGCATATATCTTCATCACATTCTCCATGTGGTTCTTAGTGATTTCTTGGTTATTTGCCCCCTTCCTCTTCAACCCTTCGGGATTTGAATGGCAAAAAATAGTGGAAGATTGGGATGATTGGTCAAAATGGATAACTAGCCGTGGTGGTATTGGTGTGCCAGCAAATAAGAGCTGGGAGTCCTGGTGGGATGAGGAACAGGAGCACCTGCAGCACACTGGGATGTTGGGACGGTTTTGGGAGATTGTACTTTCTCTGCGCTTCTTTCTTTTCCAATATGGAATTGTCTACCATCTCAATGTTTCCAGGGGTGATAAAAGTATCATGGTTTATGCTCTGTCATGGCTCGTCATTGTTGCTGTGATGATTATCCTCAAGATTGTATCCTTGGGTAGAAAAAGGTTCAGTGCCGATTTCCAGCTGATGTTCAGACTTCTGAAGTTGTTCGTCTTCATTGGGTTTGTCGTCACCATTGCAGTACTTTTTATTTTCCTTAGTCTCACTGTTGGTGACATCTTTGTAAGCTTACTGGCCTTCTTGCCTACTGGGTGGGCAATACTTATGATATCACAAGCGTGCAGGCCATTGGTGAAGGTCTTAGGAATGTGGGGGTCTGTAAAAGCTCTAGCAAGAGGGTACGAGTACGTGATGGGACTGGTTATCTTTACTCCGGTGGCTGTTCTGGCATGGTTCCCATTTGTCTCAGAGTTCCAGACCAGGCTGCTATTCAACCAGGCTTTCAGTCGAGGCCTTCAGATCCAACGTATTCTTTCTGGTGGGAAGAAGCAAAAGTCCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

869

Amino Acids

99.8

Weight (kDa)

8.87

Isoelectric Point (pI)

38.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CALS1	PF25968	20 - 203	3.1e-68	Callose synthase 1 domain
Glucan_synthase	PF02364	210 - 297	2.5e-27	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	301 - 445	6.3e-28	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	447 - 683	1.9e-50	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1415
AarI	CACCTGC	1 cut(s)	2025
AatII	GACGTC	1 cut(s)	1499
Acc36I	ACCTGC	2 cut(s)	541, 2025
AccB1I	GGYRCC	1 cut(s)	585
AccBSI	CCGCTC	1 cut(s)	865
AccI	GTMKAC	3 cut(s)	1356, 1375, 2097
AciI	CCGC	3 cut(s)	142, 395, 865
AclWI	GGATC	4 cut(s)	251, 451, 1092, 2560
AcoI	YGGCCR	3 cut(s)	56, 478, 1672
AcsI	RAATTY	3 cut(s)	341, 673, 1136
AcuI	CTGAAG	5 cut(s)	12, 989, 1019, 2267, 2546
AcyI	GRCGYC	2 cut(s)	586, 1496
AfiI	CCNNNNNNNGG	3 cut(s)	466, 787, 1239
AflII	CTTAAG	1 cut(s)	209
AflIII	ACRYGT	1 cut(s)	202
AjnI	CCWGG	5 cut(s)	1749, 1991, 2114, 2525, 2541
AleI	CACNNNNGTG	1 cut(s)	2383
Alw21I	GWGCWC	3 cut(s)	703, 997, 2016
Alw26I	GTCTC	4 cut(s)	752, 940, 2312, 2515
AlwI	GGATC	4 cut(s)	251, 451, 1092, 2560
AlwNI	CAGNNNCTG	1 cut(s)	1127
AoxI	GGCC	8 cut(s)	56, 315, 478, 1672, 1747, 2340, 2390, 2557
ApoI	RAATTY	3 cut(s)	341, 673, 1136
Asp700I	GAANNNNTTC	3 cut(s)	658, 833, 2252
AspLEI	GCGC	2 cut(s)	588, 2070
AsuNHI	GCTAGC	2 cut(s)	74, 1579
BaeI	ACNNNNGTAYC	2 cut(s)	1602, 1635
BalI	TGGCCA	2 cut(s)	58, 480
BanI	GGYRCC	1 cut(s)	585
BanII	GRGCYC	1 cut(s)	997
BarI	GAAGNNNNNNTAC	2 cut(s)	1023, 1055
BauI	CACGAG	1 cut(s)	435
BbsI	GAAGAC	2 cut(s)	1412, 2251
Bbv12I	GWGCWC	3 cut(s)	703, 997, 2016
BccI	CCATC	9 cut(s)	365, 439, 600, 781, 794, 1213, 1796, 2109, 2452
BceAI	ACGGC	3 cut(s)	1043, 1659, 1935
BcgI	CGANNNNNNTGC	2 cut(s)	1549, 1583
BciT130I	CCWGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
BciVI	GTATCC	1 cut(s)	2203
BclI	TGATCA	1 cut(s)	1509
BcoDI	GTCTC	4 cut(s)	752, 940, 2312, 2515
BfaI	CTAG	7 cut(s)	75, 1043, 1115, 1428, 1580, 1947, 2435
BfmI	CTRYAG	5 cut(s)	748, 958, 1574, 1714, 2018
BfoI	RGCGCY	1 cut(s)	589
BfrI	CTTAAG	1 cut(s)	209
BfuAI	ACCTGC	2 cut(s)	541, 2025
BfuI	GTATCC	1 cut(s)	2203
BlpI	GCTNAGC	2 cut(s)	1792, 1807
BmcAI	AGTACT	1 cut(s)	2289
Bme1390I	CCNGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
BmiI	GGNNCC	3 cut(s)	18, 587, 2501
BmrFI	CCNGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
BmrI	ACTGGG	3 cut(s)	1616, 2037, 2364
BmsI	GCATC	5 cut(s)	261, 514, 708, 874, 1592
BmtI	GCTAGC	2 cut(s)	78, 1583
BmuI	ACTGGG	3 cut(s)	1616, 2037, 2364
BpiI	GAAGAC	2 cut(s)	1412, 2251
BpmI	CTGGAG	2 cut(s)	537, 1772
Bpu1102I	GCTNAGC	2 cut(s)	1792, 1807
BpuEI	CTTGAG	2 cut(s)	803, 2168
BsaAI	YACGTR	2 cut(s)	650, 2455
BsaBI	GATNNNNATC	1 cut(s)	2177
BsaHI	GRCGYC	2 cut(s)	586, 1496
BsaI	GGTCTC	1 cut(s)	940
BsaJI	CCNNGG	4 cut(s)	1675, 1951, 2115, 2196
BsaWI	WCCGGW	1 cut(s)	2479
BsaXI	ACNNNNNCTCC	4 cut(s)	749, 779, 1979, 2009
Bsc4I	CCNNNNNNNGG	3 cut(s)	466, 787, 1239
Bse1I	ACTGG	9 cut(s)	502, 554, 1336, 1611, 1675, 2032, 2343, 2359, 2469
Bse3DI	GCAATG	1 cut(s)	2280
Bse8I	GATNNNNATC	1 cut(s)	2177
BseBI	CCWGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
BseDI	CCNNGG	4 cut(s)	1675, 1951, 2115, 2196
BseGI	GGATG	7 cut(s)	380, 889, 1206, 1725, 1930, 2005, 2037
BseJI	GATNNNNATC	1 cut(s)	2177
BseLI	CCNNNNNNNGG	3 cut(s)	466, 787, 1239
BseMI	GCAATG	1 cut(s)	2280
BseMII	CTCAG	4 cut(s)	1252, 1806, 1821, 2526
BseNI	ACTGG	9 cut(s)	502, 554, 1336, 1611, 1675, 2032, 2343, 2359, 2469
BseRI	GAGGAG	2 cut(s)	540, 770
BseYI	CCCAGC	1 cut(s)	1983
BsgI	GTGCAG	1 cut(s)	2407
Bsh1285I	CGRYCG	1 cut(s)	540
BshFI	GGCC	8 cut(s)	58, 317, 480, 1674, 1749, 2342, 2392, 2559
BshNI	GGYRCC	1 cut(s)	585
BsiEI	CGRYCG	1 cut(s)	540
BsiHKAI	GWGCWC	3 cut(s)	703, 997, 2016
BsiSI	CCGG	2 cut(s)	1093, 2480
BslFI	GGGAC	3 cut(s)	1568, 2052, 2475
BslI	CCNNNNNNNGG	3 cut(s)	466, 787, 1239
BsmAI	GTCTC	4 cut(s)	752, 940, 2312, 2515
BsmFI	GGGAC	3 cut(s)	1568, 2052, 2475
BsnI	GGCC	8 cut(s)	58, 317, 480, 1674, 1749, 2342, 2392, 2559
Bso31I	GGTCTC	1 cut(s)	940
Bsp1286I	GDGCHC	3 cut(s)	703, 997, 2016
Bsp1720I	GCTNAGC	2 cut(s)	1792, 1807
BspACI	CCGC	3 cut(s)	142, 395, 865
BspANI	GGCC	8 cut(s)	58, 317, 480, 1674, 1749, 2342, 2392, 2559
BspCNI	CTCAG	4 cut(s)	1251, 1805, 1820, 2525
BspLI	GGNNCC	3 cut(s)	18, 587, 2501
BspMAI	CTGCAG	3 cut(s)	962, 1578, 2022
BspMI	ACCTGC	2 cut(s)	541, 2025
BspOI	GCTAGC	2 cut(s)	78, 1583
BspPI	GGATC	4 cut(s)	251, 451, 1092, 2560
BspQI	GCTCTTC	1 cut(s)	1082
BspT107I	GGYRCC	1 cut(s)	585
BspTI	CTTAAG	1 cut(s)	209
BspTNI	GGTCTC	1 cut(s)	940
BsrBI	CCGCTC	1 cut(s)	865
BsrDI	GCAATG	1 cut(s)	2280
BsrI	ACTGG	9 cut(s)	502, 554, 1336, 1611, 1675, 2032, 2343, 2359, 2469
BssECI	CCNNGG	4 cut(s)	1675, 1951, 2115, 2196
BssNI	GRCGYC	2 cut(s)	586, 1496
BssSI	CACGAG	1 cut(s)	435
BssT1I	CCWWGG	1 cut(s)	2196
Bst2BI	CACGAG	1 cut(s)	435
Bst2UI	CCWGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
Bst4CI	ACNGT	7 cut(s)	607, 763, 967, 1066, 1354, 2043, 2314
Bst6I	CTCTTC	4 cut(s)	900, 1029, 1082, 1883
BstACI	GRCGYC	2 cut(s)	586, 1496
BstAFI	CTTAAG	1 cut(s)	209
BstAPI	GCANNNNNTGC	1 cut(s)	533
BstBAI	YACGTR	2 cut(s)	650, 2455
BstC8I	GCNNGC	6 cut(s)	72, 76, 1581, 1971, 2386, 2390
BstDEI	CTNAG	8 cut(s)	1238, 1656, 1792, 1807, 1846, 2303, 2407, 2512
BstDSI	CCRYGG	2 cut(s)	1675, 1951
BstENI	CCTNNNNNAGG	1 cut(s)	1237
BstF5I	GGATG	7 cut(s)	380, 889, 1206, 1725, 1930, 2005, 2037
BstH2I	RGCGCY	1 cut(s)	589
BstHHI	GCGC	2 cut(s)	588, 2070
BstMAI	GTCTC	4 cut(s)	752, 940, 2312, 2515
BstMCI	CGRYCG	1 cut(s)	540
BstMWI	GCNNNNNNNGC	6 cut(s)	409, 486, 533, 1576, 2020, 2492
BstNI	CCWGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
BstNSI	RCATGY	1 cut(s)	206
BstSCI	CCNGG	5 cut(s)	1749, 1991, 2114, 2525, 2541
BstSFI	CTRYAG	5 cut(s)	748, 958, 1574, 1714, 2018
BstV2I	GAAGAC	2 cut(s)	1412, 2251
BstX2I	RGATCY	3 cut(s)	456, 1097, 2565
BstXI	CCANNNNNNTGG	3 cut(s)	846, 1166, 1513
BstYI	RGATCY	3 cut(s)	456, 1097, 2565
BsuI	GTATCC	1 cut(s)	2203
BsuRI	GGCC	8 cut(s)	58, 317, 480, 1674, 1749, 2342, 2392, 2559
BtgI	CCRYGG	2 cut(s)	1675, 1951
BtsCI	GGATG	7 cut(s)	380, 889, 1206, 1725, 1930, 2005, 2037
BtsI	GCAGTG	1 cut(s)	1128
BtsIMutI	CAGTG	7 cut(s)	59, 495, 1128, 1604, 2025, 2221, 2310
BveI	ACCTGC	2 cut(s)	541, 2025
Cac8I	GCNNGC	6 cut(s)	72, 76, 1581, 1971, 2386, 2390
CaiI	CAGNNNCTG	1 cut(s)	1127
CfoI	GCGC	2 cut(s)	588, 2070
CseI	GACGC	1 cut(s)	914
CspCI	CAANNNNNGTGG	2 cut(s)	48, 83
DdeI	CTNAG	8 cut(s)	1238, 1656, 1792, 1807, 1846, 2303, 2407, 2512
DinI	GGCGCC	1 cut(s)	587
EaeI	YGGCCR	3 cut(s)	56, 478, 1672
Eam1104I	CTCTTC	4 cut(s)	900, 1029, 1082, 1883
EarI	CTCTTC	4 cut(s)	900, 1029, 1082, 1883
Ecl136II	GAGCTC	1 cut(s)	995
Eco130I	CCWWGG	1 cut(s)	2196
Eco147I	AGGCCT	2 cut(s)	1749, 2559
Eco24I	GRGCYC	1 cut(s)	997
Eco31I	GGTCTC	1 cut(s)	940
Eco32I	GATATC	1 cut(s)	2376
Eco53kI	GAGCTC	1 cut(s)	995
Eco57I	CTGAAG	5 cut(s)	12, 989, 1019, 2267, 2546
EcoICRI	GAGCTC	1 cut(s)	995
EcoNI	CCTNNNNNAGG	1 cut(s)	1237
EcoRII	CCWGG	5 cut(s)	1749, 1991, 2114, 2525, 2541
EcoRV	GATATC	1 cut(s)	2376
EcoT14I	CCWWGG	1 cut(s)	2196
EcoT38I	GRGCYC	1 cut(s)	997
EgeI	GGCGCC	1 cut(s)	587
EheI	GGCGCC	1 cut(s)	587
ErhI	CCWWGG	1 cut(s)	2196
FaqI	GGGAC	3 cut(s)	1568, 2052, 2475
FbaI	TGATCA	1 cut(s)	1509
FblI	GTMKAC	3 cut(s)	1356, 1375, 2097
FokI	GGATG	7 cut(s)	367, 896, 1193, 1732, 1937, 2012, 2044
FriOI	GRGCYC	1 cut(s)	997
FspBI	CTAG	7 cut(s)	75, 1043, 1115, 1428, 1580, 1947, 2435
GlaI	GCGC	2 cut(s)	587, 2069
GsaI	CCCAGC	1 cut(s)	1987
GsuI	CTGGAG	2 cut(s)	537, 1772
HaeII	RGCGCY	1 cut(s)	589
HaeIII	GGCC	8 cut(s)	58, 317, 480, 1674, 1749, 2342, 2392, 2559
HapII	CCGG	2 cut(s)	1093, 2480
HgaI	GACGC	1 cut(s)	914
HhaI	GCGC	2 cut(s)	588, 2070
Hin1I	GRCGYC	2 cut(s)	586, 1496
Hin6I	GCGC	2 cut(s)	586, 2068
HinP1I	GCGC	2 cut(s)	586, 2068
HincII	GTYRAC	3 cut(s)	603, 1324, 1494
HindII	GTYRAC	3 cut(s)	603, 1324, 1494
HindIII	AAGCTT	2 cut(s)	1001, 2332
HpaII	CCGG	2 cut(s)	1093, 2480
Hpy166II	GTNNAC	7 cut(s)	603, 1297, 1324, 1357, 1376, 1494, 2098
Hpy188III	TCNNGA	9 cut(s)	136, 299, 364, 437, 1016, 1210, 1890, 2185, 2521
Hpy8I	GTNNAC	7 cut(s)	603, 1297, 1324, 1357, 1376, 1494, 2098
Hpy99I	CGWCG	2 cut(s)	993, 1197
HpyCH4III	ACNGT	7 cut(s)	607, 763, 967, 1066, 1354, 2043, 2314
HpyCH4IV	ACGT	4 cut(s)	649, 1496, 2454, 2572
HpyF10VI	GCNNNNNNNGC	6 cut(s)	409, 486, 533, 1576, 2020, 2492
HpyF3I	CTNAG	8 cut(s)	1238, 1656, 1792, 1807, 1846, 2303, 2407, 2512
HpySE526I	ACGT	4 cut(s)	649, 1496, 2454, 2572
Hsp92I	GRCGYC	2 cut(s)	586, 1496
HspAI	GCGC	2 cut(s)	586, 2068
KasI	GGCGCC	1 cut(s)	585
Ksp22I	TGATCA	1 cut(s)	1509
LguI	GCTCTTC	1 cut(s)	1082
LmnI	GCTCC	4 cut(s)	706, 862, 1753, 2011
LweI	GCATC	5 cut(s)	261, 514, 708, 874, 1592
MaeI	CTAG	7 cut(s)	75, 1043, 1115, 1428, 1580, 1947, 2435
MaeII	ACGT	4 cut(s)	649, 1496, 2454, 2572
MaeIII	GTNAC	8 cut(s)	275, 430, 556, 776, 1060, 1733, 2275, 2318
MbiI	CCGCTC	1 cut(s)	865
MfeI	CAATTG	1 cut(s)	621
MflI	RGATCY	3 cut(s)	456, 1097, 2565
MhlI	GDGCHC	3 cut(s)	703, 997, 2016
MlsI	TGGCCA	2 cut(s)	58, 480
MluNI	TGGCCA	2 cut(s)	58, 480
Mly113I	GGCGCC	1 cut(s)	586
MlyI	GAGTC	7 cut(s)	119, 200, 623, 733, 1371, 1741, 1997
MmeI	TCCRAC	3 cut(s)	205, 402, 2593
Mox20I	TGGCCA	2 cut(s)	58, 480
MroXI	GAANNNNTTC	3 cut(s)	658, 833, 2252
MscI	TGGCCA	2 cut(s)	58, 480
MseI	TTAA	6 cut(s)	210, 261, 609, 870, 1134, 1224
MslI	CAYNNNNRTG	6 cut(s)	689, 1511, 1626, 2030, 2106, 2383
Msp20I	TGGCCA	2 cut(s)	58, 480
MspA1I	CMGCKG	4 cut(s)	922, 1573, 1811, 2230
MspCI	CTTAAG	1 cut(s)	209
MspI	CCGG	2 cut(s)	1093, 2480
MspR9I	CCNGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
MunI	CAATTG	1 cut(s)	621
MvaI	CCWGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
MwoI	GCNNNNNNNGC	6 cut(s)	409, 486, 533, 1576, 2020, 2492
NarI	GGCGCC	1 cut(s)	586
NheI	GCTAGC	2 cut(s)	74, 1579
NlaIV	GGNNCC	3 cut(s)	18, 587, 2501
NmuCI	GTSAC	6 cut(s)	430, 776, 1060, 1733, 2275, 2318
NspI	RCATGY	1 cut(s)	206
OliI	CACNNNNGTG	1 cut(s)	2383
PaqCI	CACCTGC	1 cut(s)	2025
PceI	AGGCCT	2 cut(s)	1749, 2559
PciI	ACATGT	1 cut(s)	202
PciSI	GCTCTTC	1 cut(s)	1082
PdmI	GAANNNNTTC	3 cut(s)	658, 833, 2252
PfeI	GAWTC	3 cut(s)	377, 1213, 1762
Ple19I	CGATCG	1 cut(s)	540
PleI	GAGTC	7 cut(s)	119, 199, 622, 733, 1371, 1740, 1996
PluTI	GGCGCC	1 cut(s)	589
PpsI	GAGTC	7 cut(s)	119, 199, 622, 733, 1371, 1740, 1996
Ppu21I	YACGTR	2 cut(s)	650, 2455
PscI	ACATGT	1 cut(s)	202
PsiI	TTATAA	1 cut(s)	1415
Psp124BI	GAGCTC	1 cut(s)	997
Psp6I	CCWGG	5 cut(s)	1749, 1991, 2114, 2525, 2541
PspFI	CCCAGC	1 cut(s)	1983
PspGI	CCWGG	5 cut(s)	1749, 1991, 2114, 2525, 2541
PspN4I	GGNNCC	3 cut(s)	18, 587, 2501
PstI	CTGCAG	3 cut(s)	962, 1578, 2022
PstNI	CAGNNNCTG	1 cut(s)	1127
PsuI	RGATCY	3 cut(s)	456, 1097, 2565
PvuI	CGATCG	1 cut(s)	540
PvuII	CAGCTG	4 cut(s)	922, 1573, 1811, 2230
RseI	CAYNNNNRTG	6 cut(s)	689, 1511, 1626, 2030, 2106, 2383
SacI	GAGCTC	1 cut(s)	997
SapI	GCTCTTC	1 cut(s)	1082
SaqAI	TTAA	6 cut(s)	210, 261, 609, 870, 1134, 1224
ScaI	AGTACT	1 cut(s)	2289
SchI	GAGTC	7 cut(s)	119, 200, 623, 733, 1371, 1741, 1997
ScrFI	CCNGG	5 cut(s)	1751, 1993, 2116, 2527, 2543
SduI	GDGCHC	3 cut(s)	703, 997, 2016
SfaNI	GCATC	5 cut(s)	261, 514, 708, 874, 1592
SfcI	CTRYAG	5 cut(s)	748, 958, 1574, 1714, 2018
SfoI	GGCGCC	1 cut(s)	587
SmiMI	CAYNNNNRTG	6 cut(s)	689, 1511, 1626, 2030, 2106, 2383
SmlI	CTYRAG	3 cut(s)	209, 782, 2183
SmoI	CTYRAG	3 cut(s)	209, 782, 2183
SseBI	AGGCCT	2 cut(s)	1749, 2559
SsiI	CCGC	3 cut(s)	142, 395, 865
SspDI	GGCGCC	1 cut(s)	585
SspMI	CTAG	7 cut(s)	75, 1043, 1115, 1428, 1580, 1947, 2435
SstI	GAGCTC	1 cut(s)	997
StuI	AGGCCT	2 cut(s)	1749, 2559
StyD4I	CCNGG	5 cut(s)	1749, 1991, 2114, 2525, 2541
StyI	CCWWGG	1 cut(s)	2196
TaaI	ACNGT	7 cut(s)	607, 763, 967, 1066, 1354, 2043, 2314
TaiI	ACGT	4 cut(s)	652, 1499, 2457, 2575
TaqI	TCGA	1 cut(s)	2554
TatI	WGTACW	4 cut(s)	1659, 1722, 2056, 2287
TauI	GCSGC	1 cut(s)	868
TfiI	GAWTC	3 cut(s)	377, 1213, 1762
Tru1I	TTAA	6 cut(s)	210, 261, 609, 870, 1134, 1224
Tru9I	TTAA	6 cut(s)	210, 261, 609, 870, 1134, 1224
TscAI	CASTG	7 cut(s)	59, 502, 1128, 1611, 2032, 2221, 2317
TseFI	GTSAC	6 cut(s)	430, 776, 1060, 1733, 2275, 2318
Tsp45I	GTSAC	6 cut(s)	430, 776, 1060, 1733, 2275, 2318
TspGWI	ACGGA	1 cut(s)	1744
TspRI	CASTG	7 cut(s)	59, 502, 1128, 1611, 2032, 2221, 2317
Vha464I	CTTAAG	1 cut(s)	209
XagI	CCTNNNNNAGG	1 cut(s)	1237
XapI	RAATTY	3 cut(s)	341, 673, 1136
XceI	RCATGY	1 cut(s)	206
XmiI	GTMKAC	3 cut(s)	1356, 1375, 2097
XmnI	GAANNNNTTC	3 cut(s)	658, 833, 2252
XspI	CTAG	7 cut(s)	75, 1043, 1115, 1428, 1580, 1947, 2435
ZraI	GACGTC	1 cut(s)	1497
ZrmI	AGTACT	1 cut(s)	2289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.