Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Reverse (-)

33331683 .. 33346545

Gene Structure 14863 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G023080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5631 bp

ATGGCGGCTCTAGATTCCGGCCCCCAGGGGCTGACGCGACGGCCGTCACGGAGCGCCGCGACGACGACGTTTTCGACGGAGGTCTTCGACAACGAGGTCGTCCCCTCCGCCATCGCCCCTATCGCTCCGCTCCTCCGCGTCGCCAACGAAGTCGAGCCCGAGCGCCCTCGCGTCGCCTATCTCTGTCGGTTCTACGCGTTTGAGAAGGCGCACAGATTGGACCCCGGCTCCAGCGGCCGTGGTGTGAGGCAGTTCAAGACCTTGCTCCTGCAGCGATTGGAGAGGGAAAATGCATCAAGTCTTTCTGCTCGAGTTAAAAAGTCTGATGCCAGGGAAATTACAAGCTTCTATCAGCAATATTATGATCACTATGTTAAAGCTGTTCAAGAGGAGCAGGTAGACAGAGCTCAAGTGAGTAAAGCCTACAATACACTTAACCAGCTTTTTGAAGTACTTTGCGGGGTTAACAAGTCAGAGAAAGTTGAAGAAGTTGCTCCTGAGATTATTGCAGCCGCTAGAGATGTCCAAGAGAAGACGGAAATATACGCCCCGTATAATATTCTTCCTTTGGATTCTGCTGGTGCTACACAGTCTGTCATGCAGCTTGACGAGGTTAAGGCCGCTGTGGCTGCACTGTGGAATACTCGTGGTTTAAACTGGCCAAGTGCATTCGAGCAGCACAGACAAAAAGCTAATGATTTGGATCTCCTTGATTGGCTTAGGGCCATGTTTGGGTTCCAGAGAGATAATGTCAGGAACCAGAGGGAGCATTTGATATTGCTACTTGCGAATACGCATATAAGACTTCATCCCAAACCTGAACCTCTTAACAAGCTTGATGATCGAGCTGTTGATGCAGTTCTGGACAAGCTTTTTAAGAACTACAAAACATGGTGTAAATTTTTGGGTCGAAAACATAGTTTAAGGCTACCCCAAGGTCAGCAAGAAGTACAACAGAGGAAGATACTTTACATGGGTTTATATCTTCTCATATGGGGTGAAGCAGCGAATGTTCGCTTCATGCCTGAATGCCTCTGCTATATTTTCCATAATATGGCATATGAACTCCATGGTCTGTTGGCTGGAAATGTCAGCATTGTCACTGGGGAAAATATAAAGCCTTCTTATGGTGGGGATGATGAGGCCTTTTTGAGGAAGGTTATAACCCCCCTATACCGTGTAATCGAAAAGGAAGCCAAGAAGGGCGGCGGTGGAAAAGCTCCTCACACAGCTTGGTGCAACTATGATGATTTAAATGAATATTTCTGGTCATCTGATTGTTTCTCTCTTGGTTGGCCCATGCGTGATGATGGGGATTTTTTTAAATCAACTCGTGACTTGGCACAGGGAAGAAAGGGCTCTAAAAGAAAATCTGGAAGCACAGGCAAATCATATTTTATTGAAACTCGCACATTTTGGCATATCTTTCGAAGTTTTGATCGAATGTGGACCTTTTATATACTGGCTCTACAGGCTATGCTCATTATTGCATTCAGCAAGGTTTCACCGCTGGATATATTTCAACAGGATGTCCTAAAAGATCTATCAAGTATTTTCATTACAGCAGCATTTCTTCGCGTCCTTCAGAGTATTCTGGATATGGTTTTGAACTTCCCTGGATATCACAGGTGTCGGTTCACAGATGTCCTGAGAAATATTCTGAAGATAGTTGTTAGTCTTGCATGGGCAGTCATTCTTCCACTTTTCTATGTGCATTCCTTCAATGATTCCCCTCAACAAATTGCAGATATACTGTCATTCCTTAAGAACATTCAAGGCGTTCCATCATTATATCTATTGGCTGTTGCAGTATATTTGCTTCCAAATTTACTGACAGCAGTTTTGTTCCTTTTCCCAATGCTCCGTCGTTTTATTGAAAATTCAGACTGGCATATCATCAGGTTCCTCTTGTGGTGGTCACAGCCAAGAATATATGTTGGAAGAGGAATGCATGAAAGTCAATTTGCCCTTCTAAAGTATACTTTTTTCTGGTTGCTACTTTTGGCTTGCAAGTTTACAGTTAGCTATTTTGTCCAGATAAAACCTCTGGTAGAGCCAACAGAAGACATAATGAAGATTCGTCGTATTAATTATGAGTGGCATGAATTTTTCCCTAATGCTGAACATAACTATGGAGCAGTTGTCTCACTCTGGGCACCTGTTATCATGGTTTATTTCATGGACACACAAATTTGGTATGCTATATTCCAAACATTATATGGAGGTATTGTTGGCGCCTTTGATCGCCTCGGAGAGATTCGTACTCTAGGCATGCTAAGATCCCGGTTCCAGTCTCTACCTGGTGCATTCAACACATATCTAGTGCCCTCGGATAAGTCAACAAAAAGGGGATTCTCTTTCTCAAAGAAATTTGCGGAGGCTTCAGCAAGTAGGAGAAGTGAAGCTGCAAAGTTTGCTCAGTTGTGGAATGAAGTGATATGTAGCTTTCCATTAGACATGGCAGCTCAATTCAAAAACAAGGACTCTGATCTCTGGAAGCGGATATGTGGAGATGAATATATGAAGTGTGCTGTGATTGAATGTTATGAGTCTTTCAAACATGTCCTTAAGGCTTTGGTGGTTGGAGAAAATGAGAAAAGGATCATTGGCATCATTATTAAGGAAATTGAAAGTAACATTTCCAAGAATACATTTCTTGATAATTTTAGAATGGCCTCTTTACCCACTCTTTGCCAGAAATTTGTAGAGCTTGTGGTGATCTTGAAAGATGGTGATTCATCCAAGCGATCTGCGGTGGTGCTGCTGCTGCAAGATATGTTGGAAGTTGTCACTCGTGATATGATGGTGAATGAGATCCGAGAGGTGGTTGAAGTTGGTCATAGTAGCAAGGACACTGGAAGGCAACTTTTTGCTGGTACTGATGCAAAACCTGCGATCGTGTTCCCTCCTCCAGTTACAGCACAATGGGAAGAACAGATAAGGCGCCTCCATCTGCTGTTGACTGTTAAGGAGTCTGCAATTGATGTACCAATAAACCTTGAAGCACGTAGAAGGATTTCATTCTTTGCAAATTCATTGTTCATGGATATGCCCCGTGCTCCCCGTGTTCGTAAGATGCTTTCGTTCAGCATCTTGACTCCCTACTATAGTGAGGAGACTGTGTATTCCAAAAGTGACCTTGAGATGGAAAATGAAGATGGTGTATCAATCATATATTACTTGCAGAAGATTTTCCCAGATGAATGGACTAACTTCATGGAGCGGCTTAATTGTAAAAAGGATGCTGAAATATGGGAAAATGAAGAGAACATTTTGCAGCTGCGTCATTGGGTCTCCTTACGAGGACAAACTCTCTGCAGGACAGTTAGAGGAATGATGTATTACCGACGAGCTCTGAAGCTTCAGGCTTTTCTTGATATGGCTAATGAAGAAGAGATACTAGAAGGGTACAAAGCCGTCACAGTTTCACCAGAGGACGAAAAGAAGAGCCGGAGATCCCTATTTGCTCAACTAGAAGCAGTGGCTGATCTTAAATTTACCTATGTTGCTACTTGCCAAAACTATGGCAATCAGAAGCGAAGTGGAGATCGTCGTGCAACTGACATCTTGAATCTGATGGTTAATAATCCCTCTCTCAGGGTTGCATATATTGATGAAGTTGAAGAACGAGAAGGTGAAAAGGTGCAAAAGGTTTACTATTCTGTATTGGTCAAAGCAGTTGACAACCTTGACCAGGAAATATATCGTATAAAATTGCCAGGCTCAGCAAAGATTGGAGAAGGGAAGCCTGAAAACCAGAACCATGCAGTAATCTTTACTCGTGGAGAAGCTCTTCAGGCTATTGACATGAATCAGGACAATTACTTGGAAGAAGCTCTTAAGATGCGTAACCTTCTTGAAGAATTTAATGAGGATCACGGAGTGCGTTCGCCTTCAATTTTAGGTGTTCGTGAGCATATCTTTACTGGAAGTGTTTCTTCGTTGGCGTGGTTTATGTCAAATCAAGAAATGAGCTTCGTGACCATCGGTCAAAGAGTTCTTGCTAGACCTCTTAAGGTTCGGTTCCATTATGGACATCCAGATGTCTTTGATAGAATCTTCCATATTACTCGTGGTGGCATGAGCAAGGCTTCTCGTGGGATCAATCTCAGCGAGGATATTTTTGCAGGTTTCAATTCCACTCTAAGAAGAGGAAATGTCACTCACCATGAGTACATCCAAGTTGGGAAGGGTAGGGATGTTGGCCTCAATCAAATCTCTCTCTTTGAGGCTAAAGTTGCTTGCGGTAATGGAGAGCAGACACTTAGCAGAGACATCTATCGATTAGGGCACCGTTATGATTTCTTCCGCATGTTGTCGTATTACTTTTCGACAATAGGATTTTATATCAGCTCAATGTTGGTTGTGATGACGGTCTACTCTTTCCTGTATGGTAGACTCTACTTGTCATTGAGTGGAATGGAGAAGACAATTATAACCTATGCTGCTAGAAGGGGAAATAATGCTCTACAAACAGCTATGGCTTCACAATCTGTTGTCCAATTAGGTTTGTTGACGTCCTTGCCCATGATCATGGAAATCGGACTTGAAAGAGGTTTCAGAACTGCATTAGGGGACACGGTAATAATGCAGCTGCAGCTAGCATCAGTTTTCTTTACTTTCTCACTGGGTACAAAGGTTCATTACTATGGGAGAACAATCCTGCATGGTGGGGCTAAGTACAGAGCAACTGGCCGTGGCTTTGTTGTTCGGCATGAAAGGTTTGGTGAAAACTACAGGATGTACTCACGGAGTCACTTTACAAAAGGCCTGGAGCTTATGATACTGCTTATTATTTATAGTATATATGGCTCAGCAGCAGATGGCTCAGCTGCATATATCTTCATCTCATTCTCCATGTGGTTCTTAGTGATTTCTTGGTTATTTGCCCCCTTCCTCTTCAACCCTTCGGGATTTGAATGGCAAAAAATAGTGGAAGATTGGGATGATTGGTCAAAATGGATAACTAGCCGTGGTGGTATTGGTGTGCCAGCAAATAAGAGCTGGGAGTCCTGGTGGGATGAGGAACAGGAGCACCTGCAGCACACTGGGATGTTGGGACGGTTTTGGGAGATTGTACTTTCTCTGCGCTTCTTTCTTTTCCAATATGGAATTGTCTACCATCTCAATGTTTCCAGGGGTGATAAAAGTATCATGGTTTATGCTCTGTCATGGCTCGTCATTGTTGCTGTGATGATTATCCTCAAGATTGTATCCTTGGGTAGAAAAAGGTTCAGTGCTGATTTCCAGCTGATGTTCAGACTTCTGAAGTTGTTCGTCTTCATTGGGTTTGTCGTCACCATTGCAGTACTTTTTATTTTCCTTAGTCTCACTGTTGGTGACATCTTTGTAAGCTTACTGGCCTTCTTGCCTACTGGGTGGGCAATACTTATGATATCACAAGCATGCAGGCCATTGGTGAAGGTCTTAGGAATGTGGGCGTCTGTAAAAGCTCTAGCAAGAGGGTACGAGTACGTGATGGGACTGGTTATCTTTACCCCAGTGGCTGTTCTGGCATGGTTCCCATTTGTCTCAGAGTTCCAGACCAGGCTGCTATTCAACCAGGCTTTCAGTCGAGGCCTTCAGATCCAACGTATTCTTTCTGGTGGGAAGAAGCAAAAGTCCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1876

Amino Acids

215.58

Weight (kDa)

9.04

Isoelectric Point (pI)

43.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vta1	PF04652	41 - 162	1.3e-12	Vta1 like
FKS1_dom1	PF14288	321 - 432	8e-41	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	818 - 989	3.9e-59	Callose synthase 1 domain
Glucan_synthase	PF02364	997 - 1081	7.9e-27	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	1087 - 1690	1.3e-206	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1163, 4436
AarI	CACCTGC	1 cut(s)	5046
AasI	GACNNNNNNGTC	1 cut(s)	95
AatII	GACGTC	1 cut(s)	4520
Acc36I	ACCTGC	4 cut(s)	385, 2899, 4118, 5046
AccB1I	GGYRCC	4 cut(s)	2155, 2234, 2943, 4290
AccB7I	CCANNNNNTGG	1 cut(s)	1054
AccBSI	CCGCTC	2 cut(s)	130, 3223
AccI	GTMKAC	5 cut(s)	399, 1979, 4377, 4396, 5118
AccII	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
AclWI	GGATC	8 cut(s)	711, 2274, 2609, 2809, 3450, 3882, 4109, 5581
AcoI	YGGCCR	4 cut(s)	41, 235, 659, 4693
AcuI	CTGAAG	8 cut(s)	1568, 1682, 2367, 3347, 3377, 3779, 5288, 5567
AcyI	GRCGYC	4 cut(s)	2235, 2944, 4517, 5441
AdeI	CACNNNGTG	1 cut(s)	3883
AfiI	CCNNNNNNNGG	8 cut(s)	732, 1054, 1127, 1152, 2824, 3145, 3597, 3694
AflII	CTTAAG	4 cut(s)	1763, 2567, 3839, 4013
AflIII	ACRYGT	2 cut(s)	195, 2560
AhdI	GACNNNNNGTC	1 cut(s)	3987
Alw21I	GWGCWC	4 cut(s)	409, 3061, 3355, 5037
Alw26I	GTCTC	7 cut(s)	2149, 2298, 3110, 3298, 4266, 5333, 5536
AlwI	GGATC	8 cut(s)	711, 2274, 2609, 2809, 3450, 3882, 4109, 5581
AlwNI	CAGNNNCTG	3 cut(s)	31, 3485, 5507
Ama87I	CYCGRG	2 cut(s)	158, 309
ArsI	GACNNNNNNTTYG	4 cut(s)	55, 87, 1825, 1857
AseI	ATTAAT	1 cut(s)	2088
Asp700I	GAANNNNTTC	5 cut(s)	3016, 3191, 3792, 3934, 5273
AspLEI	GCGC	6 cut(s)	56, 165, 211, 2237, 2946, 5091
AspS9I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
AsuC2I	CCSGG	2 cut(s)	225, 2284
AsuII	TTCGAA	1 cut(s)	1429
AsuNHI	GCTAGC	1 cut(s)	4600
AvaI	CYCGRG	2 cut(s)	158, 309
AvaII	GGWCC	2 cut(s)	220, 1449
BaeGI	GKGCMC	3 cut(s)	2158, 2328, 4293
BaeI	ACNNNNGTAYC	2 cut(s)	4623, 4656
BalI	TGGCCA	1 cut(s)	661
BanI	GGYRCC	4 cut(s)	2155, 2234, 2943, 4290
BanII	GRGCYC	4 cut(s)	159, 409, 1361, 3355
BarI	GAAGNNNNNNTAC	6 cut(s)	953, 985, 1803, 1835, 3381, 3413
BauI	CACGAG	6 cut(s)	645, 1332, 2793, 3780, 4071, 4095
BbsI	GAAGAC	5 cut(s)	76, 539, 2070, 4433, 5272
Bbv12I	GWGCWC	4 cut(s)	409, 3061, 3355, 5037
BceAI	ACGGC	6 cut(s)	28, 56, 222, 3401, 4680, 4956
BcgI	CGANNNNNNTGC	2 cut(s)	1409, 1443
BciVI	GTATCC	1 cut(s)	5224
BclI	TGATCA	2 cut(s)	364, 4530
BcnI	CCSGG	2 cut(s)	225, 2284
BcoDI	GTCTC	7 cut(s)	2149, 2298, 3110, 3298, 4266, 5333, 5536
BfmI	CTRYAG	7 cut(s)	269, 1469, 3106, 3316, 4595, 4735, 5039
BfoI	RGCGCY	4 cut(s)	57, 166, 2238, 2947
BfrI	CTTAAG	4 cut(s)	1763, 2567, 3839, 4013
BfuAI	ACCTGC	4 cut(s)	385, 2899, 4118, 5046
BfuI	GTATCC	1 cut(s)	5224
BglI	GCCNNNNNGGC	1 cut(s)	626
BglII	AGATCT	1 cut(s)	1540
BlpI	GCTNAGC	3 cut(s)	3724, 4813, 4828
BmcAI	AGTACT	2 cut(s)	453, 5310
Bme18I	GGWCC	2 cut(s)	220, 1449
BmeRI	GACNNNNNGTC	1 cut(s)	3987
BmeT110I	CYCGRG	2 cut(s)	158, 309
BmgT120I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
BmrI	ACTGGG	5 cut(s)	1113, 4637, 5058, 5385, 5495
BmtI	GCTAGC	1 cut(s)	4604
BmuI	ACTGGG	5 cut(s)	1113, 4637, 5058, 5385, 5495
BoxI	GACNNNNGTC	2 cut(s)	43, 80
BpiI	GAAGAC	5 cut(s)	76, 539, 2070, 4433, 5272
BpmI	CTGGAG	3 cut(s)	214, 2895, 4793
Bpu10I	CCTNAGC	1 cut(s)	719
Bpu1102I	GCTNAGC	3 cut(s)	3724, 4813, 4828
Bpu14I	TTCGAA	1 cut(s)	1429
BpuEI	CTTGAG	3 cut(s)	393, 3161, 5189
BpuMI	CCSGG	2 cut(s)	225, 2284
Bsa29I	ATCGAT	1 cut(s)	4282
BsaAI	YACGTR	2 cut(s)	3008, 5476
BsaBI	GATNNNNATC	2 cut(s)	702, 5198
BsaHI	GRCGYC	4 cut(s)	2235, 2944, 4517, 5441
BsaI	GGTCTC	1 cut(s)	3298
Bsc4I	CCNNNNNNNGG	8 cut(s)	732, 1054, 1127, 1152, 2824, 3145, 3597, 3694
Bse3DI	GCAATG	1 cut(s)	5301
Bse8I	GATNNNNATC	2 cut(s)	702, 5198
BseCI	ATCGAT	1 cut(s)	4282
BseJI	GATNNNNATC	2 cut(s)	702, 5198
BseLI	CCNNNNNNNGG	8 cut(s)	732, 1054, 1127, 1152, 2824, 3145, 3597, 3694
BseMI	GCAATG	1 cut(s)	5301
BseMII	CTCAG	9 cut(s)	489, 1640, 2432, 3610, 3738, 4123, 4827, 4842, 5547
BseRI	GAGGAG	5 cut(s)	122, 404, 1212, 2898, 3128
BseSI	GKGCMC	3 cut(s)	2158, 2328, 4293
BseX3I	CGGCCG	2 cut(s)	41, 235
BseYI	CCCAGC	1 cut(s)	5004
BsgI	GTGCAG	1 cut(s)	615
Bsh1236I	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
Bsh1285I	CGRYCG	3 cut(s)	44, 238, 2898
BshNI	GGYRCC	4 cut(s)	2155, 2234, 2943, 4290
BshVI	ATCGAT	1 cut(s)	4282
BsiEI	CGRYCG	3 cut(s)	44, 238, 2898
BsiHKAI	GWGCWC	4 cut(s)	409, 3061, 3355, 5037
BsiHKCI	CYCGRG	2 cut(s)	158, 309
BsiSI	CCGG	4 cut(s)	18, 225, 2284, 3451
BslFI	GGGAC	4 cut(s)	86, 4589, 5073, 5496
BslI	CCNNNNNNNGG	8 cut(s)	732, 1054, 1127, 1152, 2824, 3145, 3597, 3694
BsmAI	GTCTC	7 cut(s)	2149, 2298, 3110, 3298, 4266, 5333, 5536
BsmFI	GGGAC	4 cut(s)	86, 4589, 5073, 5496
BsmI	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2306
Bso31I	GGTCTC	1 cut(s)	3298
BsoBI	CYCGRG	2 cut(s)	158, 309
Bsp119I	TTCGAA	1 cut(s)	1429
Bsp1286I	GDGCHC	9 cut(s)	159, 409, 1361, 2158, 2328, 3061, 3355, 4293, 5037
Bsp1720I	GCTNAGC	3 cut(s)	3724, 4813, 4828
Bsp19I	CCATGG	1 cut(s)	1069
BspCNI	CTCAG	9 cut(s)	490, 1641, 2431, 3609, 3737, 4122, 4826, 4841, 5546
BspDI	ATCGAT	1 cut(s)	4282
BspFNI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BspMAI	CTGCAG	4 cut(s)	273, 3320, 4599, 5043
BspMI	ACCTGC	4 cut(s)	385, 2899, 4118, 5046
BspOI	GCTAGC	1 cut(s)	4604
BspPI	GGATC	8 cut(s)	711, 2274, 2609, 2809, 3450, 3882, 4109, 5581
BspQI	GCTCTTC	2 cut(s)	3440, 3798
BspT104I	TTCGAA	1 cut(s)	1429
BspT107I	GGYRCC	4 cut(s)	2155, 2234, 2943, 4290
BspTI	CTTAAG	4 cut(s)	1763, 2567, 3839, 4013
BspTNI	GGTCTC	1 cut(s)	3298
BsrBI	CCGCTC	2 cut(s)	130, 3223
BsrDI	GCAATG	1 cut(s)	5301
BssNAI	GTATAC	1 cut(s)	1980
BssNI	GRCGYC	4 cut(s)	2235, 2944, 4517, 5441
BssSI	CACGAG	6 cut(s)	645, 1332, 2793, 3780, 4071, 4095
BssT1I	CCWWGG	3 cut(s)	934, 1069, 5217
Bst1107I	GTATAC	1 cut(s)	1980
Bst2BI	CACGAG	6 cut(s)	645, 1332, 2793, 3780, 4071, 4095
Bst6I	CTCTTC	7 cut(s)	1936, 3258, 3387, 3440, 3798, 4144, 4904
BstACI	GRCGYC	4 cut(s)	2235, 2944, 4517, 5441
BstAFI	CTTAAG	4 cut(s)	1763, 2567, 3839, 4013
BstAPI	GCANNNNNTGC	2 cut(s)	2414, 2891
BstBAI	YACGTR	2 cut(s)	3008, 5476
BstBI	TTCGAA	1 cut(s)	1429
BstC8I	GCNNGC	7 cut(s)	2008, 2273, 4243, 4602, 4992, 5407, 5411
BstDSI	CCRYGG	4 cut(s)	238, 1069, 4696, 4972
BstENI	CCTNNNNNAGG	3 cut(s)	1150, 3595, 3692
BstFNI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BstH2I	RGCGCY	4 cut(s)	57, 166, 2238, 2947
BstHHI	GCGC	6 cut(s)	56, 165, 211, 2237, 2946, 5091
BstMAI	GTCTC	7 cut(s)	2149, 2298, 3110, 3298, 4266, 5333, 5536
BstMCI	CGRYCG	3 cut(s)	44, 238, 2898
BstNSI	RCATGY	4 cut(s)	2275, 2564, 4315, 5409
BstPAI	GACNNNNGTC	2 cut(s)	43, 80
BstSFI	CTRYAG	7 cut(s)	269, 1469, 3106, 3316, 4595, 4735, 5039
BstSLI	GKGCMC	3 cut(s)	2158, 2328, 4293
BstUI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BstV2I	GAAGAC	5 cut(s)	76, 539, 2070, 4433, 5272
BstX2I	RGATCY	6 cut(s)	703, 1540, 2279, 2814, 3455, 5586
BstXI	CCANNNNNNTGG	3 cut(s)	3204, 3524, 4534
BstYI	RGATCY	6 cut(s)	703, 1540, 2279, 2814, 3455, 5586
BstZ17I	GTATAC	1 cut(s)	1980
BstZI	CGGCCG	2 cut(s)	41, 235
Bsu15I	ATCGAT	1 cut(s)	4282
BsuI	GTATCC	1 cut(s)	5224
BsuTUI	ATCGAT	1 cut(s)	4282
BtgI	CCRYGG	4 cut(s)	238, 1069, 4696, 4972
BtgZI	GCGATG	1 cut(s)	97
BtsI	GCAGTG	1 cut(s)	3486
BtsIMutI	CAGTG	9 cut(s)	632, 1101, 2853, 3486, 4625, 5046, 5242, 5331, 5508
BveI	ACCTGC	4 cut(s)	385, 2899, 4118, 5046
Cac8I	GCNNGC	7 cut(s)	2008, 2273, 4243, 4602, 4992, 5407, 5411
CaiI	CAGNNNCTG	3 cut(s)	31, 3485, 5507
CfoI	GCGC	6 cut(s)	56, 165, 211, 2237, 2946, 5091
Cfr13I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
ClaI	ATCGAT	1 cut(s)	4282
CseI	GACGC	6 cut(s)	43, 127, 160, 1567, 3272, 5430
CsiI	ACCWGGT	1 cut(s)	2299
DinI	GGCGCC	2 cut(s)	2236, 2945
DraI	TTTAAA	3 cut(s)	654, 1254, 1324
DraIII	CACNNNGTG	1 cut(s)	3883
DrdI	GACNNNNNNGTC	1 cut(s)	95
DriI	GACNNNNNGTC	1 cut(s)	3987
DseDI	GACNNNNNNGTC	1 cut(s)	95
EaeI	YGGCCR	4 cut(s)	41, 235, 659, 4693
EagI	CGGCCG	2 cut(s)	41, 235
Eam1104I	CTCTTC	7 cut(s)	1936, 3258, 3387, 3440, 3798, 4144, 4904
Eam1105I	GACNNNNNGTC	1 cut(s)	3987
EarI	CTCTTC	7 cut(s)	1936, 3258, 3387, 3440, 3798, 4144, 4904
EciI	GGCGGA	1 cut(s)	97
Ecl136II	GAGCTC	2 cut(s)	407, 3353
EclXI	CGGCCG	2 cut(s)	41, 235
Eco130I	CCWWGG	3 cut(s)	934, 1069, 5217
Eco147I	AGGCCT	3 cut(s)	1145, 4770, 5580
Eco24I	GRGCYC	4 cut(s)	159, 409, 1361, 3355
Eco31I	GGTCTC	1 cut(s)	3298
Eco32I	GATATC	2 cut(s)	1622, 5397
Eco47I	GGWCC	2 cut(s)	220, 1449
Eco52I	CGGCCG	2 cut(s)	41, 235
Eco53kI	GAGCTC	2 cut(s)	407, 3353
Eco57I	CTGAAG	8 cut(s)	1568, 1682, 2367, 3347, 3377, 3779, 5288, 5567
Eco88I	CYCGRG	2 cut(s)	158, 309
EcoICRI	GAGCTC	2 cut(s)	407, 3353
EcoNI	CCTNNNNNAGG	3 cut(s)	1150, 3595, 3692
EcoRV	GATATC	2 cut(s)	1622, 5397
EcoT14I	CCWWGG	3 cut(s)	934, 1069, 5217
EcoT22I	ATGCAT	2 cut(s)	295, 1953
EcoT38I	GRGCYC	4 cut(s)	159, 409, 1361, 3355
EgeI	GGCGCC	2 cut(s)	2236, 2945
EheI	GGCGCC	2 cut(s)	2236, 2945
ErhI	CCWWGG	3 cut(s)	934, 1069, 5217
FalI	AAGNNNNNCTT	4 cut(s)	3822, 3854, 3922, 3954
FaqI	GGGAC	4 cut(s)	86, 4589, 5073, 5496
FauI	CCCGC	1 cut(s)	452
FauNDI	CATATG	2 cut(s)	992, 1060
FbaI	TGATCA	2 cut(s)	364, 4530
FblI	GTMKAC	5 cut(s)	399, 1979, 4377, 4396, 5118
FriOI	GRGCYC	4 cut(s)	159, 409, 1361, 3355
GlaI	GCGC	6 cut(s)	55, 164, 210, 2236, 2945, 5090
GsaI	CCCAGC	1 cut(s)	5008
GsuI	CTGGAG	3 cut(s)	214, 2895, 4793
HaeII	RGCGCY	4 cut(s)	57, 166, 2238, 2947
HapII	CCGG	4 cut(s)	18, 225, 2284, 3451
HgaI	GACGC	6 cut(s)	43, 127, 160, 1567, 3272, 5430
HhaI	GCGC	6 cut(s)	56, 165, 211, 2237, 2946, 5091
Hin1I	GRCGYC	4 cut(s)	2235, 2944, 4517, 5441
Hin6I	GCGC	6 cut(s)	54, 163, 209, 2235, 2944, 5089
HinP1I	GCGC	6 cut(s)	54, 163, 209, 2235, 2944, 5089
HincII	GTYRAC	5 cut(s)	466, 2340, 2961, 3682, 4515
HindII	GTYRAC	5 cut(s)	466, 2340, 2961, 3682, 4515
HindIII	AAGCTT	5 cut(s)	343, 833, 869, 3359, 5353
HpaI	GTTAAC	1 cut(s)	466
HpaII	CCGG	4 cut(s)	18, 225, 2284, 3451
HpyCH4IV	ACGT	5 cut(s)	68, 3007, 4517, 5475, 5593
HpySE526I	ACGT	5 cut(s)	68, 3007, 4517, 5475, 5593
Hsp92I	GRCGYC	4 cut(s)	2235, 2944, 4517, 5441
HspAI	GCGC	6 cut(s)	54, 163, 209, 2235, 2944, 5089
KasI	GGCGCC	2 cut(s)	2234, 2943
Ksp22I	TGATCA	2 cut(s)	364, 4530
KspAI	GTTAAC	1 cut(s)	466
LguI	GCTCTTC	2 cut(s)	3440, 3798
MabI	ACCWGGT	1 cut(s)	2299
MaeII	ACGT	5 cut(s)	68, 3007, 4517, 5475, 5593
MbiI	CCGCTC	2 cut(s)	130, 3223
MfeI	CAATTG	1 cut(s)	2979
MflI	RGATCY	6 cut(s)	703, 1540, 2279, 2814, 3455, 5586
MhlI	GDGCHC	9 cut(s)	159, 409, 1361, 2158, 2328, 3061, 3355, 4293, 5037
MlsI	TGGCCA	1 cut(s)	661
MluI	ACGCGT	1 cut(s)	195
MluNI	TGGCCA	1 cut(s)	661
Mly113I	GGCGCC	2 cut(s)	2235, 2944
MlyI	GAGTC	7 cut(s)	2477, 2558, 2981, 3091, 4392, 4762, 5018
MmeI	TCCRAC	4 cut(s)	1918, 2563, 2760, 5614
Mox20I	TGGCCA	1 cut(s)	661
Mph1103I	ATGCAT	2 cut(s)	295, 1953
MroXI	GAANNNNTTC	5 cut(s)	3016, 3191, 3792, 3934, 5273
MscI	TGGCCA	1 cut(s)	661
MslI	CAYNNNNRTG	9 cut(s)	2130, 3047, 4041, 4296, 4532, 4647, 5051, 5127, 5404
Msp20I	TGGCCA	1 cut(s)	661
MspA1I	CMGCKG	7 cut(s)	234, 623, 1510, 3280, 4594, 4832, 5251
MspCI	CTTAAG	4 cut(s)	1763, 2567, 3839, 4013
MspI	CCGG	4 cut(s)	18, 225, 2284, 3451
MssI	GTTTAAAC	1 cut(s)	654
MunI	CAATTG	1 cut(s)	2979
Mva1269I	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2306
MvnI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
NarI	GGCGCC	2 cut(s)	2235, 2944
NciI	CCSGG	2 cut(s)	225, 2284
NcoI	CCATGG	1 cut(s)	1069
NdeI	CATATG	2 cut(s)	992, 1060
NheI	GCTAGC	1 cut(s)	4600
NsiI	ATGCAT	2 cut(s)	295, 1953
NspI	RCATGY	4 cut(s)	2275, 2564, 4315, 5409
NspV	TTCGAA	1 cut(s)	1429
PaeI	GCATGC	2 cut(s)	2275, 5409
PaeR7I	CTCGAG	1 cut(s)	309
PaqCI	CACCTGC	1 cut(s)	5046
PasI	CCCWGGG	1 cut(s)	25
PceI	AGGCCT	3 cut(s)	1145, 4770, 5580
PciI	ACATGT	1 cut(s)	2560
PciSI	GCTCTTC	2 cut(s)	3440, 3798
PcsI	WCGNNNNNNNCGW	2 cut(s)	41, 71
PctI	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2306
PdmI	GAANNNNTTC	5 cut(s)	3016, 3191, 3792, 3934, 5273
PflMI	CCANNNNNTGG	1 cut(s)	1054
Ple19I	CGATCG	1 cut(s)	2898
PleI	GAGTC	7 cut(s)	2477, 2557, 2980, 3091, 4392, 4761, 5017
PluTI	GGCGCC	2 cut(s)	2238, 2947
PmeI	GTTTAAAC	1 cut(s)	654
PpsI	GAGTC	7 cut(s)	2477, 2557, 2980, 3091, 4392, 4761, 5017
Ppu21I	YACGTR	2 cut(s)	3008, 5476
PscI	ACATGT	1 cut(s)	2560
PshAI	GACNNNNGTC	2 cut(s)	43, 80
PshBI	ATTAAT	1 cut(s)	2088
PsiI	TTATAA	2 cut(s)	1163, 4436
Psp124BI	GAGCTC	2 cut(s)	409, 3355
PspFI	CCCAGC	1 cut(s)	5004
PspPI	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
PspXI	VCTCGAGB	1 cut(s)	309
PstI	CTGCAG	4 cut(s)	273, 3320, 4599, 5043
PstNI	CAGNNNCTG	3 cut(s)	31, 3485, 5507
PsuI	RGATCY	6 cut(s)	703, 1540, 2279, 2814, 3455, 5586
PvuI	CGATCG	1 cut(s)	2898
PvuII	CAGCTG	4 cut(s)	3280, 4594, 4832, 5251
RseI	CAYNNNNRTG	9 cut(s)	2130, 3047, 4041, 4296, 4532, 4647, 5051, 5127, 5404
SacI	GAGCTC	2 cut(s)	409, 3355
SapI	GCTCTTC	2 cut(s)	3440, 3798
Sau96I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1449
ScaI	AGTACT	2 cut(s)	453, 5310
SchI	GAGTC	7 cut(s)	2477, 2558, 2981, 3091, 4392, 4762, 5018
SduI	GDGCHC	9 cut(s)	159, 409, 1361, 2158, 2328, 3061, 3355, 4293, 5037
SexAI	ACCWGGT	1 cut(s)	2299
SfcI	CTRYAG	7 cut(s)	269, 1469, 3106, 3316, 4595, 4735, 5039
SfoI	GGCGCC	2 cut(s)	2236, 2945
Sfr274I	CTCGAG	1 cut(s)	309
SfuI	TTCGAA	1 cut(s)	1429
SinI	GGWCC	2 cut(s)	220, 1449
SlaI	CTCGAG	1 cut(s)	309
SmiI	ATTTAAAT	1 cut(s)	1254
SmiMI	CAYNNNNRTG	9 cut(s)	2130, 3047, 4041, 4296, 4532, 4647, 5051, 5127, 5404
SmlI	CTYRAG	8 cut(s)	309, 408, 1763, 2567, 3140, 3839, 4013, 5204
SmoI	CTYRAG	8 cut(s)	309, 408, 1763, 2567, 3140, 3839, 4013, 5204
SphI	GCATGC	2 cut(s)	2275, 5409
SseBI	AGGCCT	3 cut(s)	1145, 4770, 5580
SspDI	GGCGCC	2 cut(s)	2234, 2943
SspI	AATATT	4 cut(s)	359, 559, 1262, 1657
SstI	GAGCTC	2 cut(s)	409, 3355
StuI	AGGCCT	3 cut(s)	1145, 4770, 5580
StyI	CCWWGG	3 cut(s)	934, 1069, 5217
SwaI	ATTTAAAT	1 cut(s)	1254
TaiI	ACGT	5 cut(s)	71, 3010, 4520, 5478, 5596
TaqII	GACCGA	1 cut(s)	3977
TatI	WGTACW	7 cut(s)	451, 949, 4173, 4680, 4743, 5077, 5308
TauI	GCSGC	7 cut(s)	8, 59, 237, 515, 623, 1209, 3226
TscAI	CASTG	9 cut(s)	639, 1108, 2860, 3486, 4632, 5053, 5242, 5338, 5508
TspGWI	ACGGA	6 cut(s)	64, 92, 551, 1853, 3894, 4765
TspRI	CASTG	9 cut(s)	639, 1108, 2860, 3486, 4632, 5053, 5242, 5338, 5508
Van91I	CCANNNNNTGG	1 cut(s)	1054
Vha464I	CTTAAG	4 cut(s)	1763, 2567, 3839, 4013
VpaK11BI	GGWCC	2 cut(s)	220, 1449
VspI	ATTAAT	1 cut(s)	2088
XagI	CCTNNNNNAGG	3 cut(s)	1150, 3595, 3692
XbaI	TCTAGA	1 cut(s)	10
XceI	RCATGY	4 cut(s)	2275, 2564, 4315, 5409
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2216, 2297, 4070
XhoI	CTCGAG	1 cut(s)	309
XmiI	GTMKAC	5 cut(s)	399, 1979, 4377, 4396, 5118
XmnI	GAANNNNTTC	5 cut(s)	3016, 3191, 3792, 3934, 5273
ZraI	GACGTC	1 cut(s)	4518
ZrmI	AGTACT	2 cut(s)	453, 5310
Zsp2I	ATGCAT	2 cut(s)	295, 1953

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.