AT2G16460

Protein of unknown function (DUF1640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002615
Physical Location & Seq
Reverse (-)
7133387 .. 7135812
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G16460.1

Sequence Viewer

Length: 693 bp
ATGACATTGTCCAAGCGATTGGCTCTATTGGGAGCTCAATCTGCGATTACTCTCGCTAAACCTCGAGGTCTCGGTTCCTCTCTCGGGTTACTTGATCGTCGTCCATTTCCGTATCGTCAATTTTCTGAGCTCACGAAAGCTAATGGGAGACGTGCTTTTCTCGTTGATACATTGGCTCTGGTGAGGAGTCTTGAAGCTCAAGGAGTTCCATCGAAACAAGCTGAGGCAATAACTTCTGCTATTACTGAGGTTTTGAATGATAGCTTGGAAAATGTCTCTGAGTCTTTTGTGTCGAAAGCGGAAATGCAGAAGATTGAAATGATTCAAGATTCAAATTTATCGAAATTTAAATCTGAAGTCAAAAGTTCTCAGGAGCATCATTTTACTGTATTGCAACGTGAGACTGAAAAATTGAGAGGCGATATAGAGAAGATGCGAAGTGAGCTAAGGTACGAAATTGATAAGGTCACAGCGGGACAACGCTTGGATTTGAATCTTGAAAGAGGCCGAATACGTGATGAGCTAGCTAATCAGAATACCGAAACAACAAACCTCACAAACAAGCTTGACAGGGAAATTCATGCGTTGAGAGCTCAATTAGAAGCTTCAAAATACGAAGTCATCAAATATTGCATAGGTACACTTGTGTCGATTTCAGCAGTGGGACTCGCCGTCCTTCGTATCATGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1640

Protein Analysis

230

Amino Acids

25.79

Weight (kDa)

9.16

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC90-like PF07798 55 - 229 5.5e-60 Coiled-coil domain-containing protein 90-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 63
AciI CCGC 2 cut(s) 299, 473
AcsI RAATTY 3 cut(s) 334, 344, 576
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 2 cut(s) 452, 640
AfiI CCNNNNNNNGG 1 cut(s) 84
AgsI TTSAA 8 cut(s) 194, 256, 317, 326, 333, 493, 500, 609
AhdI GACNNNNNGTC 1 cut(s) 671
AjiI CACGTC 1 cut(s) 152
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
Alw21I GWGCWC 3 cut(s) 37, 132, 595
Alw26I GTCTC 4 cut(s) 74, 142, 280, 395
Ama87I CYCGRG 2 cut(s) 63, 83
AoxI GGCC 1 cut(s) 505
ApoI RAATTY 3 cut(s) 334, 344, 576
Asp700I GAANNNNTTC 1 cut(s) 321
AsuHPI GGTGA 1 cut(s) 193
AsuNHI GCTAGC 1 cut(s) 523
AvaI CYCGRG 2 cut(s) 63, 83
BaeI ACNNNNGTAYC 2 cut(s) 630, 663
BanII GRGCYC 3 cut(s) 37, 132, 595
Bbv12I GWGCWC 3 cut(s) 37, 132, 595
BbvCI CCTCAGC 1 cut(s) 222
BccI CCATC 1 cut(s) 217
BceAI ACGGC 1 cut(s) 656
BcoDI GTCTC 4 cut(s) 74, 142, 280, 395
BfaI CTAG 1 cut(s) 524
BmeRI GACNNNNNGTC 1 cut(s) 671
BmeT110I CYCGRG 2 cut(s) 63, 83
BmgBI CACGTC 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 76
BmsI GCATC 2 cut(s) 385, 423
BmtI GCTAGC 1 cut(s) 527
Bpu10I CCTNAGC 2 cut(s) 222, 446
BpuEI CTTGAG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 74
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 492
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 84
BseMII CTCAG 5 cut(s) 117, 213, 237, 270, 383
BseRI GAGGAG 1 cut(s) 199
BshFI GGCC 1 cut(s) 507
BsiHKAI GWGCWC 3 cut(s) 37, 132, 595
BsiHKCI CYCGRG 2 cut(s) 63, 83
BslFI GGGAC 2 cut(s) 489, 678
BslI CCNNNNNNNGG 1 cut(s) 84
BsmAI GTCTC 4 cut(s) 74, 142, 280, 395
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 2 cut(s) 489, 678
BsnI GGCC 1 cut(s) 507
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 2 cut(s) 63, 83
Bsp1286I GDGCHC 3 cut(s) 37, 132, 595
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 2 cut(s) 299, 473
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 5 cut(s) 118, 214, 238, 271, 382
BspHI TCATGA 1 cut(s) 684
BspLI GGNNCC 1 cut(s) 76
BspOI GCTAGC 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 74
BssMI GATC 1 cut(s) 94
Bst4CI ACNGT 1 cut(s) 388
BstBAI YACGTR 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 6 cut(s) 126, 222, 246, 279, 369, 446
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 4 cut(s) 74, 142, 280, 395
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 3 cut(s) 41, 442, 590
BstXI CCANNNNNNTGG 1 cut(s) 19
BsuRI GGCC 1 cut(s) 507
BtrI CACGTC 1 cut(s) 152
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 1 cut(s) 666
Cac8I GCNNGC 1 cut(s) 525
CciI TCATGA 1 cut(s) 684
Csp6I GTAC 2 cut(s) 451, 639
CviAII CATG 2 cut(s) 581, 685
CviQI GTAC 2 cut(s) 451, 639
DdeI CTNAG 6 cut(s) 126, 222, 246, 279, 369, 446
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
DraI TTTAAA 1 cut(s) 349
DriI GACNNNNNGTC 1 cut(s) 671
Eam1105I GACNNNNNGTC 1 cut(s) 671
Ecl136II GAGCTC 3 cut(s) 35, 130, 593
Eco24I GRGCYC 3 cut(s) 37, 132, 595
Eco31I GGTCTC 1 cut(s) 74
Eco53kI GAGCTC 3 cut(s) 35, 130, 593
Eco57I CTGAAG 1 cut(s) 375
Eco88I CYCGRG 2 cut(s) 63, 83
EcoICRI GAGCTC 3 cut(s) 35, 130, 593
EcoT38I GRGCYC 3 cut(s) 37, 132, 595
Esp3I CGTCTC 1 cut(s) 142
FaeI CATG 2 cut(s) 584, 688
FaiI YATR 4 cut(s) 425, 582, 635, 686
FaqI GGGAC 2 cut(s) 489, 678
FatI CATG 2 cut(s) 580, 684
FauI CCCGC 1 cut(s) 466
FriOI GRGCYC 3 cut(s) 37, 132, 595
FspBI CTAG 1 cut(s) 524
HaeIII GGCC 1 cut(s) 507
Hin1II CATG 2 cut(s) 584, 688
HindIII AAGCTT 2 cut(s) 563, 603
HinfI GANTC 6 cut(s) 187, 281, 322, 329, 493, 666
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 641
Hpy188I TCNGA 4 cut(s) 127, 280, 355, 534
Hpy188III TCNNGA 6 cut(s) 133, 191, 326, 371, 497, 685
Hpy8I GTNNAC 1 cut(s) 641
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 1 cut(s) 686
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 3 cut(s) 151, 397, 514
HpyCH4V TGCA 3 cut(s) 307, 394, 633
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 442, 590
HpyF3I CTNAG 6 cut(s) 126, 222, 246, 279, 369, 446
HpySE526I ACGT 3 cut(s) 151, 397, 514
Hsp92II CATG 2 cut(s) 584, 688
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 32, 373
LpnPI CCDG 3 cut(s) 164, 356, 556
LweI GCATC 2 cut(s) 385, 423
MaeI CTAG 1 cut(s) 524
MaeII ACGT 3 cut(s) 151, 397, 514
MaeIII GTNAC 2 cut(s) 87, 466
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 2 cut(s) 322, 442
MhlI GDGCHC 3 cut(s) 37, 132, 595
MluCI AATT 7 cut(s) 119, 334, 344, 410, 456, 576, 596
MlyI GAGTC 3 cut(s) 196, 290, 660
MnlI CCTC 9 cut(s) 59, 72, 88, 177, 217, 241, 410, 497, 563
MroXI GAANNNNTTC 1 cut(s) 321
MseI TTAA 2 cut(s) 348, 691
MspA1I CMGCKG 1 cut(s) 473
MwoI GCNNNNNNNGC 3 cut(s) 41, 442, 590
NdeII GATC 1 cut(s) 94
NheI GCTAGC 1 cut(s) 523
NlaIII CATG 2 cut(s) 584, 688
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 63
PagI TCATGA 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 3 cut(s) 322, 329, 493
PflFI GACNNNGTC 1 cut(s) 7
PleI GAGTC 3 cut(s) 195, 289, 660
PpsI GAGTC 3 cut(s) 195, 289, 660
Ppu21I YACGTR 1 cut(s) 515
Psp124BI GAGCTC 3 cut(s) 37, 132, 595
PspN4I GGNNCC 1 cut(s) 76
PspXI VCTCGAGB 1 cut(s) 63
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 2 cut(s) 452, 640
RsaNI GTAC 2 cut(s) 451, 639
SacI GAGCTC 3 cut(s) 37, 132, 595
SaqAI TTAA 2 cut(s) 348, 691
Sau3AI GATC 1 cut(s) 94
SchI GAGTC 3 cut(s) 196, 290, 660
SduI GDGCHC 3 cut(s) 37, 132, 595
SfaNI GCATC 2 cut(s) 385, 423
Sfr274I CTCGAG 1 cut(s) 63
SlaI CTCGAG 1 cut(s) 63
SmiI ATTTAAAT 1 cut(s) 349
SmlI CTYRAG 2 cut(s) 63, 198
SmoI CTYRAG 2 cut(s) 63, 198
Sse9I AATT 7 cut(s) 119, 334, 344, 410, 456, 576, 596
SsiI CCGC 2 cut(s) 299, 473
SspI AATATT 1 cut(s) 629
SspMI CTAG 1 cut(s) 524
SstI GAGCTC 3 cut(s) 37, 132, 595
SwaI ATTTAAAT 1 cut(s) 349
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 3 cut(s) 154, 400, 517
TaqI TCGA 5 cut(s) 64, 212, 293, 341, 650
TasI AATT 7 cut(s) 119, 334, 344, 410, 456, 576, 596
TfiI GAWTC 3 cut(s) 322, 329, 493
Tru1I TTAA 2 cut(s) 348, 691
Tru9I TTAA 2 cut(s) 348, 691
TscAI CASTG 1 cut(s) 666
TseFI GTSAC 1 cut(s) 466
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 1 cut(s) 569
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 1 cut(s) 666
Tth111I GACNNNGTC 1 cut(s) 7
XapI RAATTY 3 cut(s) 334, 344, 576
XhoI CTCGAG 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.