FvH4_2g10260

Protein of unknown function (DUF1640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002615
Physical Location & Seq
Reverse (-)
9098782 .. 9101923
3142 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10260.t2

Sequence Viewer

Length: 663 bp
ATGGCTCTCTCAAAGCGCGCGCTCAAATCCGCCGCCGATTCCGCCTTTCTCCTACGGCGAGGCCTCTACTCTTCGTCGTCGGTCTCCACTAACGGCGTCGTCGCCCGCGCCTCCGAGCTCGTCAAGTCCGGCGGAAAACGAGCCTTTCCGGTCGACACTTTGGCTCTGGTGAGGGGCTTGGAAGCCAAAGGCGTTCCGACGGCGCATGCCGAGGCAATCACGTCTGCCGTTACCGGAATTTACACTGATAGCTTGGAGAATGTGCACCAATTGCAGAAGACTGTGATACTGCAAGAAGCGAAGCTGTCCAAGTTCGAAGTCGAAATCAAGAGTTTACAGGAACATCATTTTTCCATGTTGACACGTGAAACAGAAAAGCTTCAGAGTGTCATAGACAAGTTGCGGAGTGAACTGAGGTATGAGTTGGACAAGGTTGCAGCTGGGTCACGCTTGGATTTAAATCTTGAAAAAGGGCGCATGCGTGATGAGTTTAACAATCAGAATGCAGAAACAGCCAATCTAACAAACAAACTTGATCGAGAAATTCAAGCAGTAAAAGCCTTGGTTGAAACGGGAAAATATGAAGTGATTAAATACTGCATTGGGACCCTTGTCTCCATGACAGCAGTTGGCATTGCTGTCCTCCGTATTATGATGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1640

Protein Analysis

221

Amino Acids

24.2

Weight (kDa)

9.25

Isoelectric Point (pI)

29.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC90-like PF07798 51 - 158 1.8e-24 Coiled-coil domain-containing protein 90-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 153
AccII CGCG 3 cut(s) 18, 20, 108
AciI CCGC 6 cut(s) 30, 33, 42, 106, 132, 403
AcsI RAATTY 2 cut(s) 237, 543
AcuI CTGAAG 1 cut(s) 365
AcvI CACGTG 1 cut(s) 365
AcyI GRCGYC 1 cut(s) 96
AflIII ACRYGT 1 cut(s) 362
AgsI TTSAA 3 cut(s) 467, 548, 569
AjiI CACGTC 1 cut(s) 222
AluBI AGCT 5 cut(s) 118, 252, 304, 379, 440
AluI AGCT 5 cut(s) 118, 252, 304, 379, 440
Alw21I GWGCWC 2 cut(s) 120, 267
Alw26I GTCTC 2 cut(s) 88, 619
Alw44I GTGCAC 1 cut(s) 263
AoxI GGCC 1 cut(s) 61
ApaLI GTGCAC 1 cut(s) 263
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 2 cut(s) 237, 543
Asp700I GAANNNNTTC 1 cut(s) 378
AspLEI GCGC 6 cut(s) 18, 20, 22, 110, 205, 477
AspS9I GGNCC 1 cut(s) 606
AsuHPI GGTGA 1 cut(s) 181
AsuII TTCGAA 1 cut(s) 315
AvaII GGWCC 1 cut(s) 606
BaeGI GKGCMC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 2 cut(s) 120, 267
BbvI GCAGC 1 cut(s) 449
BceAI ACGGC 4 cut(s) 71, 109, 212, 216
BcoDI GTCTC 2 cut(s) 88, 619
BisI GCNGC 2 cut(s) 33, 438
BlsI GCNGC 2 cut(s) 34, 439
Bme18I GGWCC 1 cut(s) 606
BmgBI CACGTC 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 606
BmiI GGNNCC 2 cut(s) 607, 608
BoxI GACNNNNGTC 1 cut(s) 611
BpiI GAAGAC 1 cut(s) 284
Bpu14I TTCGAA 1 cut(s) 315
BsaAI YACGTR 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 2 cut(s) 210, 561
BsaWI WCCGGW 2 cut(s) 148, 233
Bse3DI GCAATG 1 cut(s) 633
Bse8I GATNNNNATC 1 cut(s) 459
BseDI CCNNGG 2 cut(s) 210, 561
BseJI GATNNNNATC 1 cut(s) 459
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 1 cut(s) 404
BsePI GCGCGC 2 cut(s) 16, 18
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 1 cut(s) 449
BseYI CCCAGC 1 cut(s) 440
Bsh1236I CGCG 3 cut(s) 18, 20, 108
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 2 cut(s) 120, 267
BsiSI CCGG 3 cut(s) 129, 149, 234
BslFI GGGAC 1 cut(s) 619
BsmAI GTCTC 2 cut(s) 88, 619
BsmFI GGGAC 1 cut(s) 619
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 1 cut(s) 63
Bso31I GGTCTC 1 cut(s) 88
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 2 cut(s) 120, 267
Bsp143I GATC 1 cut(s) 535
BspACI CCGC 6 cut(s) 30, 33, 42, 106, 132, 403
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 1 cut(s) 405
BspFNI CGCG 3 cut(s) 18, 20, 108
BspLI GGNNCC 2 cut(s) 607, 608
BspT104I TTCGAA 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 633
BssECI CCNNGG 2 cut(s) 210, 561
BssHII GCGCGC 2 cut(s) 16, 18
BssMI GATC 1 cut(s) 535
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 561
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 1 cut(s) 76
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 271
BstBAI YACGTR 1 cut(s) 365
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 5 cut(s) 18, 20, 106, 207, 479
BstDEI CTNAG 1 cut(s) 413
BstFNI CGCG 3 cut(s) 18, 20, 108
BstHHI GCGC 6 cut(s) 18, 20, 22, 110, 205, 477
BstKTI GATC 1 cut(s) 538
BstMAI GTCTC 2 cut(s) 88, 619
BstMBI GATC 1 cut(s) 535
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 4 cut(s) 41, 271, 512, 557
BstNSI RCATGY 2 cut(s) 209, 481
BstPAI GACNNNNGTC 1 cut(s) 611
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 3 cut(s) 18, 20, 108
BstV1I GCAGC 1 cut(s) 449
BstV2I GAAGAC 1 cut(s) 284
BsuRI GGCC 1 cut(s) 63
BtrI CACGTC 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 5 cut(s) 18, 20, 106, 207, 479
CfoI GCGC 6 cut(s) 18, 20, 22, 110, 205, 477
Cfr13I GGNCC 1 cut(s) 606
CseI GACGC 1 cut(s) 85
CviAII CATG 4 cut(s) 206, 355, 478, 619
DdeI CTNAG 1 cut(s) 413
DpnI GATC 1 cut(s) 537
DpnII GATC 1 cut(s) 535
DraI TTTAAA 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
EciI GGCGGA 3 cut(s) 19, 31, 147
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 561
Eco147I AGGCCT 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 606
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 365
Eco72I CACGTG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 606
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 4 cut(s) 209, 358, 481, 622
FaiI YATR 8 cut(s) 207, 356, 392, 420, 479, 582, 620, 653
FaqI GGGAC 1 cut(s) 619
FatI CATG 4 cut(s) 205, 354, 477, 618
FauI CCCGC 1 cut(s) 113
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 2 cut(s) 33, 438
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 2 cut(s) 33, 438
GlaI GCGC 6 cut(s) 17, 19, 21, 109, 204, 476
GluI GCNGC 2 cut(s) 33, 438
GsaI CCCAGC 1 cut(s) 444
HaeIII GGCC 1 cut(s) 63
HapII CCGG 3 cut(s) 129, 149, 234
HgaI GACGC 1 cut(s) 85
HhaI GCGC 6 cut(s) 18, 20, 22, 110, 205, 477
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 4 cut(s) 209, 358, 481, 622
Hin6I GCGC 6 cut(s) 16, 18, 20, 108, 203, 475
HinP1I GCGC 6 cut(s) 16, 18, 20, 108, 203, 475
HincII GTYRAC 2 cut(s) 154, 360
HindII GTYRAC 2 cut(s) 154, 360
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 1 cut(s) 38
HpaII CCGG 3 cut(s) 129, 149, 234
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 5 cut(s) 154, 265, 335, 360, 410
Hpy188I TCNGA 4 cut(s) 115, 198, 384, 501
Hpy188III TCNNGA 3 cut(s) 328, 464, 539
Hpy8I GTNNAC 5 cut(s) 154, 265, 335, 360, 410
Hpy99I CGWCG 5 cut(s) 79, 82, 101, 104, 202
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 2 cut(s) 221, 364
HpyCH4V TGCA 6 cut(s) 265, 274, 292, 437, 506, 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 271, 512, 557
HpyF3I CTNAG 1 cut(s) 413
HpySE526I ACGT 2 cut(s) 221, 364
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 4 cut(s) 209, 358, 481, 622
HspAI GCGC 6 cut(s) 16, 18, 20, 108, 203, 475
KflI GGGWCCC 1 cut(s) 606
Kzo9I GATC 1 cut(s) 535
LpnPI CCDG 6 cut(s) 142, 152, 162, 247, 323, 426
Lsp1109I GCAGC 1 cut(s) 449
MaeII ACGT 2 cut(s) 221, 364
MaeIII GTNAC 2 cut(s) 229, 444
MalI GATC 1 cut(s) 537
MboI GATC 1 cut(s) 535
MboII GAAGA 2 cut(s) 63, 289
MfeI CAATTG 1 cut(s) 269
MhlI GDGCHC 2 cut(s) 120, 267
MluCI AATT 4 cut(s) 237, 269, 543, 658
MmeI TCCRAC 2 cut(s) 221, 405
MnlI CCTC 7 cut(s) 53, 74, 121, 165, 205, 408, 653
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 3 cut(s) 458, 492, 591
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 3 cut(s) 129, 149, 234
MunI CAATTG 1 cut(s) 269
Mva1269I GAATGC 1 cut(s) 508
MvnI CGCG 3 cut(s) 18, 20, 108
MwoI GCNNNNNNNGC 4 cut(s) 41, 271, 512, 557
NdeII GATC 1 cut(s) 535
NlaIII CATG 4 cut(s) 209, 358, 481, 622
NlaIV GGNNCC 2 cut(s) 607, 608
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 444
NspI RCATGY 2 cut(s) 209, 481
NspV TTCGAA 1 cut(s) 315
PaeI GCATGC 2 cut(s) 209, 481
PauI GCGCGC 2 cut(s) 16, 18
PceI AGGCCT 1 cut(s) 63
PctI GAATGC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 1 cut(s) 38
PkrI GCNGC 2 cut(s) 34, 439
PmaCI CACGTG 1 cut(s) 365
PmlI CACGTG 1 cut(s) 365
Ppu21I YACGTR 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 606
PshAI GACNNNNGTC 1 cut(s) 611
Psp124BI GAGCTC 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 606
PspCI CACGTG 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 440
PspN4I GGNNCC 2 cut(s) 607, 608
PspPI GGNCC 1 cut(s) 606
PspPPI RGGWCCY 1 cut(s) 606
PteI GCGCGC 2 cut(s) 16, 18
PvuII CAGCTG 1 cut(s) 440
SacI GAGCTC 1 cut(s) 120
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 3 cut(s) 458, 492, 591
SatI GCNGC 2 cut(s) 33, 438
Sau3AI GATC 1 cut(s) 535
Sau96I GGNCC 1 cut(s) 606
SduI GDGCHC 2 cut(s) 120, 267
SetI ASST 9 cut(s) 120, 224, 254, 306, 367, 381, 419, 435, 442
SfuI TTCGAA 1 cut(s) 315
SinI GGWCC 1 cut(s) 606
SmiI ATTTAAAT 1 cut(s) 459
SphI GCATGC 2 cut(s) 209, 481
Sse9I AATT 4 cut(s) 237, 269, 543, 658
SseBI AGGCCT 1 cut(s) 63
SsiI CCGC 6 cut(s) 30, 33, 42, 106, 132, 403
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 1 cut(s) 63
StyI CCWWGG 1 cut(s) 561
SwaI ATTTAAAT 1 cut(s) 459
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 2 cut(s) 224, 367
TaqI TCGA 4 cut(s) 153, 315, 321, 538
TaqII GACCGA 1 cut(s) 70
TasI AATT 4 cut(s) 237, 269, 543, 658
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 3 cut(s) 458, 492, 591
Tru9I TTAA 3 cut(s) 458, 492, 591
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 444
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 1 cut(s) 444
TspDTI ATGAA 1 cut(s) 597
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 1 cut(s) 250
VneI GTGCAC 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 606
XapI RAATTY 2 cut(s) 237, 543
XceI RCATGY 2 cut(s) 209, 481
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.