MD00G1040600.v1.1

Protein of unknown function (DUF1640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002615
Physical Location & Seq
Forward (+)
6937234 .. 6940489
3256 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1040600.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGCGCTCTGCAAGCGGGTGCTTCAAACCGGTGCCGAATCGGCGTTTCGCTTCTCCGGCTTCCGACGAAACTTCTATGCGCCTTCCTCGACGGGCAACAGAGGACACGGACTCGTGAGCAGGCAGATTTCGCAGCTGGTCAAACCGAACGGAAACCGCGCGTTTCTTGTCGACACGCTGGCTCTGGTCAGGGGTTTGGAGGCCAAAGGCGTGCCGACGAAGCAGGCGGAGGCCATCACGGCTGCGATCACTGAAGTTCTGAATGATAGCTTGGAGAATGTAGCTCATGCTTTTGTATCTAAATCCGAAATGCAGCAGGCTGTAATGCTGCAAGAATCAAACCTGTCAAAGTTCAAATCCGAAGTACAGAGTTCACAGGAGCATCATTTTACAGTGTTGCAACGTGAAACTGAAAAACTTCGTGGTGATATAGAGAAGATGCGCAGTGAATTGAGGTACGAGATTGACAAAGTTACAGCAGGGCAGCGTTTGGATTTAAATCTTGAAAGAGGGCGCATACGCGATGAGCTTGCCAACCAAAATGCAGAAACAACTAATCTCACAAATAAACTTGACAGAGAAATTCATGCATTAAGAGCACAGGTGGAAACTGCAAAATATGAGGTGATCAAATATTGCATAGGTACTTTGGTCTCCATAACTGCTGTTGGACTCGCTGTAGTTCGTATTTTCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1640

Protein Analysis

233

Amino Acids

25.92

Weight (kDa)

9.27

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC90-like PF07798 57 - 231 9.5e-61 Coiled-coil domain-containing protein 90-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 443
AccB1I GGYRCC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 171
AccII CGCG 3 cut(s) 159, 161, 522
AciI CCGC 3 cut(s) 16, 157, 227
AcsI RAATTY 1 cut(s) 582
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 3 cut(s) 366, 458, 646
AgeI ACCGGT 1 cut(s) 29
AgsI TTSAA 3 cut(s) 26, 355, 506
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 4 cut(s) 136, 270, 284, 529
AluI AGCT 4 cut(s) 136, 270, 284, 529
Alw21I GWGCWC 1 cut(s) 601
Alw26I GTCTC 1 cut(s) 658
AoxI GGCC 2 cut(s) 201, 231
ApeKI GCWGC 5 cut(s) 133, 242, 313, 328, 484
ApoI RAATTY 1 cut(s) 582
AsiGI ACCGGT 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 417
AspLEI GCGC 5 cut(s) 7, 82, 161, 444, 516
AsuHPI GGTGA 2 cut(s) 437, 637
BaeI ACNNNNGTAYC 2 cut(s) 636, 669
BanI GGYRCC 1 cut(s) 32
BauI CACGAG 1 cut(s) 113
Bbv12I GWGCWC 1 cut(s) 601
BbvI GCAGC 5 cut(s) 145, 229, 315, 325, 496
BccI CCATC 1 cut(s) 242
BceAI ACGGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BclI TGATCA 1 cut(s) 627
BcoDI GTCTC 1 cut(s) 658
BfmI CTRYAG 1 cut(s) 678
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 2 cut(s) 41, 239
BisI GCNGC 5 cut(s) 134, 243, 314, 329, 485
BlsI GCNGC 5 cut(s) 135, 244, 315, 330, 486
BmiI GGNNCC 1 cut(s) 34
BmsI GCATC 2 cut(s) 391, 429
BsaBI GATNNNNATC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 658
BsaWI WCCGGW 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 498
BseJI GATNNNNATC 1 cut(s) 498
BseXI GCAGC 5 cut(s) 145, 229, 315, 325, 496
Bsh1236I CGCG 3 cut(s) 159, 161, 522
BshFI GGCC 2 cut(s) 203, 233
BshNI GGYRCC 1 cut(s) 32
BshTI ACCGGT 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 601
BsiSI CCGG 2 cut(s) 30, 57
BsmAI GTCTC 1 cut(s) 658
BsnI GGCC 2 cut(s) 203, 233
Bso31I GGTCTC 1 cut(s) 658
Bsp1286I GDGCHC 1 cut(s) 601
Bsp143I GATC 2 cut(s) 246, 627
BspACI CCGC 3 cut(s) 16, 157, 227
BspANI GGCC 2 cut(s) 203, 233
BspFNI CGCG 3 cut(s) 159, 161, 522
BspLI GGNNCC 1 cut(s) 34
BspT107I GGYRCC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 658
BsrFI RCCGGY 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 29
BssMI GATC 2 cut(s) 246, 627
BssSI CACGAG 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 394
BstC8I GCNNGC 7 cut(s) 14, 122, 180, 212, 225, 318, 531
BstFNI CGCG 3 cut(s) 159, 161, 522
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 5 cut(s) 7, 82, 161, 444, 516
BstKTI GATC 2 cut(s) 249, 630
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 2 cut(s) 246, 627
BstMWI GCNNNNNNNGC 7 cut(s) 13, 41, 57, 130, 220, 239, 596
BstSFI CTRYAG 1 cut(s) 678
BstUI CGCG 3 cut(s) 159, 161, 522
BstV1I GCAGC 5 cut(s) 145, 229, 315, 325, 496
BsuRI GGCC 2 cut(s) 203, 233
BtgZI GCGATG 1 cut(s) 537
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 3 cut(s) 249, 399, 451
Cac8I GCNNGC 7 cut(s) 14, 122, 180, 212, 225, 318, 531
CfoI GCGC 5 cut(s) 7, 82, 161, 444, 516
Cfr10I RCCGGY 1 cut(s) 29
Csp6I GTAC 3 cut(s) 365, 457, 645
CspAI ACCGGT 1 cut(s) 29
CviAII CATG 2 cut(s) 287, 587
CviQI GTAC 3 cut(s) 365, 457, 645
DpnI GATC 2 cut(s) 248, 629
DpnII GATC 2 cut(s) 246, 627
DraI TTTAAA 1 cut(s) 498
EciI GGCGGA 1 cut(s) 242
Eco31I GGTCTC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 592
FaeI CATG 2 cut(s) 290, 590
FaiI YATR 9 cut(s) 78, 288, 431, 518, 588, 621, 641, 659, 697
FatI CATG 2 cut(s) 286, 586
FauI CCCGC 1 cut(s) 9
FbaI TGATCA 1 cut(s) 627
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 5 cut(s) 134, 243, 314, 329, 485
Fsp4HI GCNGC 5 cut(s) 134, 243, 314, 329, 485
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 5 cut(s) 6, 81, 160, 443, 515
GluI GCNGC 5 cut(s) 134, 243, 314, 329, 485
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 203, 233
HapII CCGG 2 cut(s) 30, 57
HhaI GCGC 5 cut(s) 7, 82, 161, 444, 516
Hin1II CATG 2 cut(s) 290, 590
Hin6I GCGC 5 cut(s) 5, 80, 159, 442, 514
HinP1I GCGC 5 cut(s) 5, 80, 159, 442, 514
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 4 cut(s) 38, 111, 335, 672
HpaII CCGG 2 cut(s) 30, 57
HphI GGTGA 2 cut(s) 437, 637
Hpy166II GTNNAC 2 cut(s) 172, 374
Hpy188I TCNGA 4 cut(s) 65, 261, 307, 361
Hpy188III TCNNGA 2 cut(s) 115, 503
Hpy8I GTNNAC 2 cut(s) 172, 374
Hpy99I CGWCG 3 cut(s) 69, 94, 220
HpyAV CCTTC 1 cut(s) 93
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 403
HpyCH4V TGCA 8 cut(s) 12, 313, 331, 400, 545, 590, 614, 639
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 41, 57, 130, 220, 239, 596
HpySE526I ACGT 1 cut(s) 403
Hsp92II CATG 2 cut(s) 290, 590
HspAI GCGC 5 cut(s) 5, 80, 159, 442, 514
Ksp22I TGATCA 1 cut(s) 627
Kzo9I GATC 2 cut(s) 246, 627
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 5 cut(s) 145, 229, 315, 325, 496
LweI GCATC 2 cut(s) 391, 429
MaeII ACGT 1 cut(s) 403
MaeIII GTNAC 1 cut(s) 472
MalI GATC 2 cut(s) 248, 629
MboI GATC 2 cut(s) 246, 627
MboII GAAGA 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 601
MluCI AATT 2 cut(s) 449, 582
MlyI GAGTC 2 cut(s) 105, 666
MmeI TCCRAC 2 cut(s) 88, 649
MnlI CCTC 7 cut(s) 95, 97, 193, 223, 447, 503, 616
Mph1103I ATGCAT 1 cut(s) 592
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 2 cut(s) 497, 593
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 2 cut(s) 30, 57
MvnI CGCG 3 cut(s) 159, 161, 522
MwoI GCNNNNNNNGC 7 cut(s) 13, 41, 57, 130, 220, 239, 596
NdeII GATC 2 cut(s) 246, 627
NlaIII CATG 2 cut(s) 290, 590
NlaIV GGNNCC 1 cut(s) 34
NsbI TGCGCA 1 cut(s) 443
NsiI ATGCAT 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 2 cut(s) 38, 335
PinAI ACCGGT 1 cut(s) 29
PkrI GCNGC 5 cut(s) 135, 244, 315, 330, 486
PleI GAGTC 2 cut(s) 105, 666
PpsI GAGTC 2 cut(s) 105, 666
PspN4I GGNNCC 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 136
RsaI GTAC 3 cut(s) 366, 458, 646
RsaNI GTAC 3 cut(s) 365, 457, 645
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 497, 593
SatI GCNGC 5 cut(s) 134, 243, 314, 329, 485
Sau3AI GATC 2 cut(s) 246, 627
SchI GAGTC 2 cut(s) 105, 666
SduI GDGCHC 1 cut(s) 601
SfaNI GCATC 2 cut(s) 391, 429
SfcI CTRYAG 1 cut(s) 678
SmiI ATTTAAAT 1 cut(s) 498
Sse9I AATT 2 cut(s) 449, 582
SsiI CCGC 3 cut(s) 16, 157, 227
SspI AATATT 1 cut(s) 635
SwaI ATTTAAAT 1 cut(s) 498
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 406
TaqI TCGA 2 cut(s) 89, 171
TasI AATT 2 cut(s) 449, 582
TatI WGTACW 1 cut(s) 364
TfiI GAWTC 2 cut(s) 38, 335
Tru1I TTAA 2 cut(s) 497, 593
Tru9I TTAA 2 cut(s) 497, 593
TscAI CASTG 3 cut(s) 256, 399, 451
TseI GCWGC 5 cut(s) 133, 242, 313, 328, 484
TspDTI ATGAA 1 cut(s) 575
TspGWI ACGGA 2 cut(s) 123, 165
TspRI CASTG 3 cut(s) 256, 399, 451
XapI RAATTY 1 cut(s) 582
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.