Rroxscaffold_7G00200740

Protein of unknown function (DUF1640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002615
Physical Location & Seq
Forward (+)
48102131 .. 48105422
3292 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00200740.1

Sequence Viewer

Length: 468 bp
ATGGCTCTCTGTAAGCACGTCCTCAAGTCCGCCGCCGATTCTGCCTTTCTTCTCCGGCGAGGCCTCTACTCGTCGTCGTCGGTTTCCACTAACGGAGTCGTCACCCGCGCCTCGGAGCTAGTCAAGTCCGGCGGAAACCGAGCCTATCCGGTGGACACTCTAGCTCTGGTGAGGGGCTTGGAGGCCAAAGGCGTGCCGACGGACCATGCCGAGGCAATCACGTCTGCCATCACCGGAGTTTCCACTGATAGCTTGGAGAATGTGAACCAATTGCAGAGGACTGTGATGCTGCAAGAAGCGAAACTGTCCAAGTTTGAAGCCGAAGTAAAGAGTTTACAGGAACATCATTTTTCCATGTTGACACGTGAAACAGAAAAGCTTCAGAGTATCATAGACAAGATGCGCAGTGAACTGAGGTATGAGTTGGACAAGGTTGCAGCTGGGGTCACGCTTGGATTTAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1640

Protein Analysis

155

Amino Acids

16.87

Weight (kDa)

8.71

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC90-like PF07798 51 - 153 2.7e-20 Coiled-coil domain-containing protein 90-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 404
AccII CGCG 1 cut(s) 108
AciI CCGC 4 cut(s) 30, 33, 106, 132
AcuI CTGAAG 1 cut(s) 365
AcvI CACGTG 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 112, 211
AflIII ACRYGT 1 cut(s) 362
AgsI TTSAA 1 cut(s) 317
AjiI CACGTC 2 cut(s) 19, 222
AluBI AGCT 5 cut(s) 118, 164, 252, 379, 440
AluI AGCT 5 cut(s) 118, 164, 252, 379, 440
AoxI GGCC 2 cut(s) 61, 183
ApeKI GCWGC 2 cut(s) 289, 437
Asp700I GAANNNNTTC 1 cut(s) 378
AspLEI GCGC 2 cut(s) 110, 405
AspS9I GGNCC 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 94, 181, 223
AvaII GGWCC 1 cut(s) 202
BbrPI CACGTG 1 cut(s) 365
BbvI GCAGC 2 cut(s) 276, 449
BccI CCATC 1 cut(s) 236
BfaI CTAG 2 cut(s) 119, 161
BisI GCNGC 3 cut(s) 33, 290, 438
BlsI GCNGC 3 cut(s) 34, 291, 439
Bme18I GGWCC 1 cut(s) 202
BmgBI CACGTC 2 cut(s) 19, 222
BmgT120I GGNCC 1 cut(s) 202
BmsI GCATC 2 cut(s) 276, 390
BpuEI CTTGAG 1 cut(s) 8
BsaAI YACGTR 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 460
BsaJI CCNNGG 2 cut(s) 111, 210
BsaWI WCCGGW 2 cut(s) 148, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 211
Bse8I GATNNNNATC 1 cut(s) 460
BseDI CCNNGG 2 cut(s) 111, 210
BseJI GATNNNNATC 1 cut(s) 460
BseLI CCNNNNNNNGG 2 cut(s) 112, 211
BseMII CTCAG 1 cut(s) 404
BseXI GCAGC 2 cut(s) 276, 449
BseYI CCCAGC 1 cut(s) 440
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 63, 185
BsiSI CCGG 4 cut(s) 55, 129, 149, 234
BslI CCNNNNNNNGG 2 cut(s) 112, 211
BsnI GGCC 2 cut(s) 63, 185
BspACI CCGC 4 cut(s) 30, 33, 106, 132
BspANI GGCC 2 cut(s) 63, 185
BspCNI CTCAG 1 cut(s) 405
BspFNI CGCG 1 cut(s) 108
BssECI CCNNGG 2 cut(s) 111, 210
Bst4CI ACNGT 2 cut(s) 283, 306
BstBAI YACGTR 1 cut(s) 365
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 1 cut(s) 413
BstFNI CGCG 1 cut(s) 108
BstHHI GCGC 2 cut(s) 110, 405
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 276, 449
BsuRI GGCC 2 cut(s) 63, 185
BtrI CACGTC 2 cut(s) 19, 222
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 243, 412
Cac8I GCNNGC 1 cut(s) 194
CfoI GCGC 2 cut(s) 110, 405
Cfr13I GGNCC 1 cut(s) 202
CviAII CATG 2 cut(s) 206, 355
DdeI CTNAG 1 cut(s) 413
DraI TTTAAA 1 cut(s) 460
EciI GGCGGA 2 cut(s) 19, 147
Eco147I AGGCCT 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 365
Eco72I CACGTG 1 cut(s) 365
FaeI CATG 2 cut(s) 209, 358
FaiI YATR 4 cut(s) 207, 356, 392, 420
FatI CATG 2 cut(s) 205, 354
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 33, 290, 438
Fsp4HI GCNGC 3 cut(s) 33, 290, 438
FspBI CTAG 2 cut(s) 119, 161
FspI TGCGCA 1 cut(s) 404
GlaI GCGC 2 cut(s) 109, 404
GluI GCNGC 3 cut(s) 33, 290, 438
GsaI CCCAGC 1 cut(s) 444
HaeIII GGCC 2 cut(s) 63, 185
HapII CCGG 4 cut(s) 55, 129, 149, 234
HhaI GCGC 2 cut(s) 110, 405
Hin1II CATG 2 cut(s) 209, 358
Hin6I GCGC 2 cut(s) 108, 403
HinP1I GCGC 2 cut(s) 108, 403
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 2 cut(s) 38, 96
HpaII CCGG 4 cut(s) 55, 129, 149, 234
HphI GGTGA 3 cut(s) 94, 181, 223
Hpy166II GTNNAC 5 cut(s) 154, 265, 335, 360, 410
Hpy188I TCNGA 2 cut(s) 115, 384
Hpy188III TCNNGA 1 cut(s) 465
Hpy8I GTNNAC 5 cut(s) 154, 265, 335, 360, 410
Hpy99I CGWCG 4 cut(s) 76, 79, 82, 202
HpyCH4III ACNGT 2 cut(s) 283, 306
HpyCH4IV ACGT 3 cut(s) 18, 221, 364
HpyCH4V TGCA 3 cut(s) 274, 292, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 1 cut(s) 413
HpySE526I ACGT 3 cut(s) 18, 221, 364
Hsp92II CATG 2 cut(s) 209, 358
HspAI GCGC 2 cut(s) 108, 403
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 7 cut(s) 68, 142, 152, 162, 247, 323, 426
Lsp1109I GCAGC 2 cut(s) 276, 449
LweI GCATC 2 cut(s) 276, 390
MaeI CTAG 2 cut(s) 119, 161
MaeII ACGT 3 cut(s) 18, 221, 364
MaeIII GTNAC 2 cut(s) 100, 445
MboII GAAGA 1 cut(s) 41
MfeI CAATTG 1 cut(s) 269
MluCI AATT 1 cut(s) 269
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 9 cut(s) 32, 53, 74, 121, 165, 175, 205, 270, 408
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 4 cut(s) 55, 129, 149, 234
MunI CAATTG 1 cut(s) 269
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 41
NlaIII CATG 2 cut(s) 209, 358
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 2 cut(s) 100, 445
NsbI TGCGCA 1 cut(s) 404
PceI AGGCCT 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 1 cut(s) 38
PkrI GCNGC 3 cut(s) 34, 291, 439
PleI GAGTC 1 cut(s) 104
PmaCI CACGTG 1 cut(s) 365
PmlI CACGTG 1 cut(s) 365
PpsI GAGTC 1 cut(s) 104
Ppu21I YACGTR 1 cut(s) 365
PspCI CACGTG 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 440
PspPI GGNCC 1 cut(s) 202
PvuII CAGCTG 1 cut(s) 440
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 3 cut(s) 33, 290, 438
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 1 cut(s) 105
SfaNI GCATC 2 cut(s) 276, 390
SinI GGWCC 1 cut(s) 202
SmiI ATTTAAAT 1 cut(s) 460
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 1 cut(s) 269
SseBI AGGCCT 1 cut(s) 63
SsiI CCGC 4 cut(s) 30, 33, 106, 132
SspMI CTAG 2 cut(s) 119, 161
StuI AGGCCT 1 cut(s) 63
SwaI ATTTAAAT 1 cut(s) 460
TaaI ACNGT 2 cut(s) 283, 306
TaiI ACGT 3 cut(s) 21, 224, 367
TasI AATT 1 cut(s) 269
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 2 cut(s) 250, 412
TseFI GTSAC 2 cut(s) 100, 445
TseI GCWGC 2 cut(s) 289, 437
Tsp45I GTSAC 2 cut(s) 100, 445
TspGWI ACGGA 2 cut(s) 108, 215
TspRI CASTG 2 cut(s) 250, 412
VpaK11BI GGWCC 1 cut(s) 202
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 2 cut(s) 119, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.