AT2G16780

WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005006
Physical Location & Seq
Reverse (-)
7281374 .. 7283686
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G16780.1

Sequence Viewer

Length: 1248 bp
ATGGCAGATGAAGGTAAGGAGGAGACAGGAATGGGTCAGGTCGAGGAAGATTTTTCTGTGTGGAAGAAGAACACACCATTTCTCTACGATCTTTTGATCTCTCACCCACTCGAATGGCCATCTCTCACCGTCCATTGGGTTCCATCGACGCCAAATCCTTACGTAGCAGACTCTTACTTCGGTGTCCACAAGCTTATACTCGGTACTCATACTTCCGGTAGTGCTCAAGATTTTCTCATGGTCGCCGACGTCGTTACCCCGACACCAAACGCAGAACCTGGGATAGGCGGCGCTAATCAGGATCCCTTTATTCCTAAGGTGGAGATTAGGCAGAGGATACGTGTTGATGGAGAAGTGAACAGAGCGCGGTGTATGCCTCAAAAGCCGACGCTTGTGGGTGCAAAGACGAGTGGGTGTGAGGTGTTTCTGTTTGATTACGCCAAACATGCTGCAAAGTCTCAAACGAGTGAGTGTGATCCTGATCTGAGGCTAGTGGGACATGACAAGGAAGGGTATGGATTGTCTTGGAGTCCTTTCAAGGAAGGTTATCTTTTGAGTGGTTCACAGGATCAGAAGATCTGTCTTTGGGATGTTTCAGCTACACCGCAAGATAAGGTTTTGAATGCAATGTTTGTGTATGAGGGACATGAAAGTGCTATCGCAGATGTGTCATGGCACATGAAGAATGAGAACCTTTTTGGTTCTGCGGGTGAGGATGGCCGATTGGTGATATGGGACACACGGACAAACCAAATGCAACACCAAGTGAAAGTTCACGAGAGAGAGGTCAACTATCTATCTTTCAATCCGTTCAACGAATGGGTATTGGCCACAGCTTCCTCAGACTCCACTGTTGCTTTGTTTGACCTCCGGAAACTTAATGCGCCATTGCATGTCATGAGCAGCCATGAGGGAGAGGTCTTCCAAGTGGAGTGGGATCCTAACCATGAAACAGTACTCGCATCTTCAGGGGAGGACAGAAGGCTGATGGTCTGGGACCTGAACAGGGTTGGAGAAGAGCAGCTTGAGATAGAGTTGGATGCAGAAGATGGTCCACCAGAGTTACTCTTCTCTCACGGAGGCCACAAGGCTAAGATATCAGACTTTGCTTGGAACAAAAACGAGCCTTGGGTCATTGCAAGTGTAGCTGAAGACAACAGTCTTCAGGTGTGGCAGATGGCAGAAAGCATCTATCGCGATGAAGAGGATGCCGAGGACATCAAAGAAGATATTACACAACAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0031461 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

415

Amino Acids

46.7

Weight (kDa)

4.66

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1C_H4-bd PF12265 16 - 87 5.4e-24 Histone-binding protein RBBP4 or subunit C of CAF1 complex
WDR55 PF24796 112 - 338 7.2e-15 WDR55
WD40_CDC20-Fz PF24807 117 - 259 5.9e-13 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 141 - 263 5.3e-08 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 162 - 270 1.7e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 167 - 270 1.8e-16 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 172 - 338 5.4e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_Aladin PF25460 173 - 281 8e-07 Aladin seven-bladed propeller
WD40_Prp19 PF24814 176 - 334 2.8e-19 Prp19 WD40 domain
Beta-prop_EIPR1 PF23609 176 - 288 1.3e-11 EIPR1 beta-propeller
WD40_Gbeta PF25391 182 - 304 5.7e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 183 - 291 6e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 183 - 392 5.4e-14 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 184 - 288 7.7e-07 WDR3 second beta-propeller domain
EIF3I PF24805 189 - 294 2.2e-08 EIF3I
Beta-prop_WDR3_1st PF25173 211 - 334 8.8e-17 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 212 - 293 1.1e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 213 - 336 1.2e-18 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 214 - 336 2.9e-18 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 215 - 335 1.3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 259 - 344 4.3e-13 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 262 - 336 5.1e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 252
AccII CGCG 2 cut(s) 367, 1197
AccIII TCCGGA 1 cut(s) 870
AciI CCGC 4 cut(s) 288, 367, 605, 707
AclWI GGATC 6 cut(s) 296, 309, 470, 576, 932, 945
AcoI YGGCCR 3 cut(s) 116, 718, 828
AcuI CTGAAG 3 cut(s) 951, 1148, 1170
AcyI GRCGYC 2 cut(s) 149, 249
AdeI CACNNNGTG 1 cut(s) 766
AfaI GTAC 2 cut(s) 205, 957
AfiI CCNNNNNNNGG 3 cut(s) 135, 284, 1006
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 4 cut(s) 538, 622, 805, 814
AjnI CCWGG 1 cut(s) 277
AjuI GAANNNNNNNTTGG 2 cut(s) 756, 788
AluBI AGCT 5 cut(s) 193, 599, 836, 1024, 1148
AluI AGCT 5 cut(s) 193, 599, 836, 1024, 1148
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 2 cut(s) 17, 462
AlwI GGATC 6 cut(s) 296, 309, 470, 576, 932, 945
AlwNI CAGNNNCTG 1 cut(s) 278
Aor13HI TCCGGA 1 cut(s) 870
AoxI GGCC 4 cut(s) 116, 718, 828, 1081
ApeKI GCWGC 3 cut(s) 449, 903, 1021
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspLEI GCGC 3 cut(s) 293, 367, 886
AspS9I GGNCC 2 cut(s) 997, 1052
AsuHPI GGTGA 4 cut(s) 95, 118, 722, 739
AvaII GGWCC 2 cut(s) 997, 1052
AxyI CCTNAGG 1 cut(s) 315
BalI TGGCCA 2 cut(s) 118, 830
BamHI GGATCC 2 cut(s) 301, 937
BarI GAAGNNNNNNTAC 2 cut(s) 196, 228
BauI CACGAG 1 cut(s) 776
BbsI GAAGAC 3 cut(s) 913, 1154, 1158
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 3 cut(s) 436, 915, 1033
BccI CCATC 7 cut(s) 127, 151, 341, 710, 982, 1043, 1171
BciT130I CCWGG 1 cut(s) 279
BciVI GTATCC 1 cut(s) 330
BcoDI GTCTC 2 cut(s) 17, 462
BfaI CTAG 1 cut(s) 491
BfoI RGCGCY 1 cut(s) 294
BfuI GTATCC 1 cut(s) 330
BglII AGATCT 1 cut(s) 576
BisI GCNGC 4 cut(s) 289, 450, 904, 1022
BlsI GCNGC 4 cut(s) 290, 451, 905, 1023
BmcAI AGTACT 1 cut(s) 957
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 2 cut(s) 997, 1052
BmgT120I GGNCC 2 cut(s) 997, 1052
BmiI GGNNCC 4 cut(s) 141, 303, 939, 998
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 4 cut(s) 971, 1030, 1197, 1198
BoxI GACNNNNGTC 1 cut(s) 1158
BpiI GAAGAC 3 cut(s) 913, 1154, 1158
BpuEI CTTGAG 2 cut(s) 210, 1046
BsaAI YACGTR 2 cut(s) 163, 341
BsaBI GATNNNNATC 1 cut(s) 480
BsaHI GRCGYC 2 cut(s) 149, 249
BsaJI CCNNGG 3 cut(s) 278, 1127, 1212
BsaWI WCCGGW 2 cut(s) 215, 870
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 284, 1006
Bse21I CCTNAGG 1 cut(s) 315
Bse3DI GCAATG 3 cut(s) 633, 887, 1134
Bse8I GATNNNNATC 1 cut(s) 480
BseAI TCCGGA 1 cut(s) 870
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 3 cut(s) 278, 1127, 1212
BseGI GGATG 4 cut(s) 595, 721, 1045, 1213
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 3 cut(s) 135, 284, 1006
BseMI GCAATG 3 cut(s) 633, 887, 1134
BseMII CTCAG 2 cut(s) 476, 855
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 3 cut(s) 436, 915, 1033
Bsh1236I CGCG 2 cut(s) 367, 1197
BshFI GGCC 4 cut(s) 118, 720, 830, 1083
BsiHKAI GWGCWC 1 cut(s) 226
BsiSI CCGG 2 cut(s) 216, 871
BslFI GGGAC 4 cut(s) 510, 657, 749, 1010
BslI CCNNNNNNNGG 3 cut(s) 135, 284, 1006
BsmAI GTCTC 2 cut(s) 17, 462
BsmFI GGGAC 4 cut(s) 510, 657, 749, 1010
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 4 cut(s) 118, 720, 830, 1083
Bsp1286I GDGCHC 1 cut(s) 226
Bsp13I TCCGGA 1 cut(s) 870
Bsp143I GATC 8 cut(s) 88, 96, 301, 475, 481, 568, 576, 937
Bsp68I TCGCGA 1 cut(s) 1197
BspACI CCGC 4 cut(s) 288, 367, 605, 707
BspANI GGCC 4 cut(s) 118, 720, 830, 1083
BspCNI CTCAG 2 cut(s) 477, 854
BspEI TCCGGA 1 cut(s) 870
BspFNI CGCG 2 cut(s) 367, 1197
BspHI TCATGA 2 cut(s) 897, 1244
BspLI GGNNCC 4 cut(s) 141, 303, 939, 998
BspPI GGATC 6 cut(s) 296, 309, 470, 576, 932, 945
BspQI GCTCTTC 1 cut(s) 1011
BsrDI GCAATG 3 cut(s) 633, 887, 1134
BssECI CCNNGG 3 cut(s) 278, 1127, 1212
BssMI GATC 8 cut(s) 88, 96, 301, 475, 481, 568, 576, 937
BssNI GRCGYC 2 cut(s) 149, 249
BssSI CACGAG 1 cut(s) 776
BssT1I CCWWGG 1 cut(s) 1127
Bst2BI CACGAG 1 cut(s) 776
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 4 cut(s) 130, 853, 955, 1160
Bst6I CTCTTC 3 cut(s) 1011, 1073, 1197
BstACI GRCGYC 2 cut(s) 149, 249
BstBAI YACGTR 2 cut(s) 163, 341
BstDEI CTNAG 4 cut(s) 315, 485, 841, 1092
BstENI CCTNNNNNAGG 1 cut(s) 282
BstF5I GGATG 4 cut(s) 595, 721, 1045, 1213
BstFNI CGCG 2 cut(s) 367, 1197
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 3 cut(s) 293, 367, 886
BstKTI GATC 8 cut(s) 91, 99, 304, 478, 484, 571, 579, 940
BstMAI GTCTC 2 cut(s) 17, 462
BstMBI GATC 8 cut(s) 88, 96, 301, 475, 481, 568, 576, 937
BstMWI GCNNNNNNNGC 5 cut(s) 373, 382, 446, 1145, 1194
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 2 cut(s) 449, 896
BstPAI GACNNNNGTC 1 cut(s) 1158
BstSCI CCNGG 1 cut(s) 277
BstSNI TACGTA 1 cut(s) 163
BstUI CGCG 2 cut(s) 367, 1197
BstV1I GCAGC 3 cut(s) 436, 915, 1033
BstV2I GAAGAC 3 cut(s) 913, 1154, 1158
BstX2I RGATCY 3 cut(s) 301, 576, 937
BstXI CCANNNNNNTGG 1 cut(s) 114
BstYI RGATCY 3 cut(s) 301, 576, 937
Bsu36I CCTNAGG 1 cut(s) 315
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 4 cut(s) 118, 720, 830, 1083
BtgZI GCGATG 1 cut(s) 1212
BtsCI GGATG 4 cut(s) 595, 721, 1045, 1213
BtsIMutI CAGTG 1 cut(s) 849
BtuMI TCGCGA 1 cut(s) 1197
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 2 cut(s) 897, 1244
CfoI GCGC 3 cut(s) 293, 367, 886
Cfr13I GGNCC 2 cut(s) 997, 1052
CseI GACGC 2 cut(s) 157, 397
Csp6I GTAC 2 cut(s) 204, 956
CspCI CAANNNNNGTGG 2 cut(s) 914, 949
CviQI GTAC 2 cut(s) 204, 956
DdeI CTNAG 4 cut(s) 315, 485, 841, 1092
DpnI GATC 8 cut(s) 90, 98, 303, 477, 483, 570, 578, 939
DpnII GATC 8 cut(s) 88, 96, 301, 475, 481, 568, 576, 937
DraIII CACNNNGTG 1 cut(s) 766
EaeI YGGCCR 3 cut(s) 116, 718, 828
Eam1104I CTCTTC 3 cut(s) 1011, 1073, 1197
EarI CTCTTC 3 cut(s) 1011, 1073, 1197
Eco105I TACGTA 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 1127
Eco32I GATATC 1 cut(s) 1098
Eco47I GGWCC 2 cut(s) 997, 1052
Eco57I CTGAAG 3 cut(s) 951, 1148, 1170
Eco81I CCTNAGG 1 cut(s) 315
EcoNI CCTNNNNNAGG 1 cut(s) 282
EcoO109I RGGNCCY 1 cut(s) 997
EcoRII CCWGG 1 cut(s) 277
EcoRV GATATC 1 cut(s) 1098
EcoT14I CCWWGG 1 cut(s) 1127
ErhI CCWWGG 1 cut(s) 1127
FalI AAGNNNNNCTT 4 cut(s) 534, 566, 1008, 1040
FaqI GGGAC 4 cut(s) 510, 657, 749, 1010
FauI CCCGC 1 cut(s) 700
Fnu4HI GCNGC 4 cut(s) 289, 450, 904, 1022
FokI GGATG 4 cut(s) 602, 728, 1052, 1220
Fsp4HI GCNGC 4 cut(s) 289, 450, 904, 1022
FspBI CTAG 1 cut(s) 491
GlaI GCGC 3 cut(s) 292, 366, 885
GluI GCNGC 4 cut(s) 289, 450, 904, 1022
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 4 cut(s) 118, 720, 830, 1083
HapII CCGG 2 cut(s) 216, 871
HgaI GACGC 2 cut(s) 157, 397
HhaI GCGC 3 cut(s) 293, 367, 886
Hin1I GRCGYC 2 cut(s) 149, 249
Hin6I GCGC 3 cut(s) 291, 365, 884
HinP1I GCGC 3 cut(s) 291, 365, 884
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 3 cut(s) 170, 529, 845
HpaII CCGG 2 cut(s) 216, 871
HphI GGTGA 4 cut(s) 95, 118, 722, 739
Hpy166II GTNNAC 6 cut(s) 187, 358, 563, 775, 790, 1055
Hpy188I TCNGA 4 cut(s) 486, 573, 844, 1102
Hpy188III TCNNGA 8 cut(s) 227, 299, 479, 776, 871, 898, 1196, 1245
Hpy8I GTNNAC 6 cut(s) 187, 358, 563, 775, 790, 1055
Hpy99I CGWCG 4 cut(s) 151, 251, 254, 391
HpyAV CCTTC 4 cut(s) 5, 503, 536, 975
HpyCH4III ACNGT 4 cut(s) 130, 853, 955, 1160
HpyCH4IV ACGT 3 cut(s) 162, 249, 340
HpyCH4V TGCA 7 cut(s) 401, 452, 626, 757, 892, 1043, 1139
HpyF10VI GCNNNNNNNGC 5 cut(s) 373, 382, 446, 1145, 1194
HpyF3I CTNAG 4 cut(s) 315, 485, 841, 1092
HpySE526I ACGT 3 cut(s) 162, 249, 340
Hsp92I GRCGYC 2 cut(s) 149, 249
HspAI GCGC 3 cut(s) 291, 365, 884
Kpn2I TCCGGA 1 cut(s) 870
Kzo9I GATC 8 cut(s) 88, 96, 301, 475, 481, 568, 576, 937
LguI GCTCTTC 1 cut(s) 1011
Lsp1109I GCAGC 3 cut(s) 436, 915, 1033
LweI GCATC 4 cut(s) 971, 1030, 1197, 1198
MaeI CTAG 1 cut(s) 491
MaeII ACGT 3 cut(s) 162, 249, 340
MaeIII GTNAC 2 cut(s) 253, 1062
MalI GATC 8 cut(s) 90, 98, 303, 477, 483, 570, 578, 939
MboI GATC 8 cut(s) 88, 96, 301, 475, 481, 568, 576, 937
MflI RGATCY 3 cut(s) 301, 576, 937
MhlI GDGCHC 1 cut(s) 226
MlsI TGGCCA 2 cut(s) 118, 830
MluNI TGGCCA 2 cut(s) 118, 830
MlyI GAGTC 3 cut(s) 164, 538, 839
MmeI TCCRAC 2 cut(s) 991, 1017
Mox20I TGGCCA 2 cut(s) 118, 830
MroI TCCGGA 1 cut(s) 870
MscI TGGCCA 2 cut(s) 118, 830
MseI TTAA 1 cut(s) 879
MslI CAYNNNNRTG 2 cut(s) 112, 651
Msp20I TGGCCA 2 cut(s) 118, 830
MspI CCGG 2 cut(s) 216, 871
MspR9I CCNGG 1 cut(s) 279
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 2 cut(s) 367, 1197
MwoI GCNNNNNNNGC 5 cut(s) 373, 382, 446, 1145, 1194
NdeII GATC 8 cut(s) 88, 96, 301, 475, 481, 568, 576, 937
NlaIV GGNNCC 4 cut(s) 141, 303, 939, 998
NmeAIII GCCGAG 1 cut(s) 1237
NruI TCGCGA 1 cut(s) 1197
NspI RCATGY 2 cut(s) 449, 896
PagI TCATGA 2 cut(s) 897, 1244
PciSI GCTCTTC 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PctI GAATGC 1 cut(s) 628
PkrI GCNGC 4 cut(s) 290, 451, 905, 1023
PleI GAGTC 3 cut(s) 164, 537, 839
PpsI GAGTC 3 cut(s) 164, 537, 839
Ppu21I YACGTR 2 cut(s) 163, 341
PpuMI RGGWCCY 1 cut(s) 997
PshAI GACNNNNGTC 1 cut(s) 1158
Psp5II RGGWCCY 1 cut(s) 997
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 4 cut(s) 141, 303, 939, 998
PspPI GGNCC 2 cut(s) 997, 1052
PspPPI RGGWCCY 1 cut(s) 997
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 3 cut(s) 301, 576, 937
RruI TCGCGA 1 cut(s) 1197
RsaI GTAC 2 cut(s) 205, 957
RsaNI GTAC 2 cut(s) 204, 956
RseI CAYNNNNRTG 2 cut(s) 112, 651
SapI GCTCTTC 1 cut(s) 1011
SaqAI TTAA 1 cut(s) 879
SatI GCNGC 4 cut(s) 289, 450, 904, 1022
Sau3AI GATC 8 cut(s) 88, 96, 301, 475, 481, 568, 576, 937
Sau96I GGNCC 2 cut(s) 997, 1052
ScaI AGTACT 1 cut(s) 957
SchI GAGTC 3 cut(s) 164, 538, 839
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 1 cut(s) 226
SfaNI GCATC 4 cut(s) 971, 1030, 1197, 1198
SinI GGWCC 2 cut(s) 997, 1052
SmiMI CAYNNNNRTG 2 cut(s) 112, 651
SmlI CTYRAG 2 cut(s) 225, 1025
SmoI CTYRAG 2 cut(s) 225, 1025
SnaBI TACGTA 1 cut(s) 163
SsiI CCGC 4 cut(s) 288, 367, 605, 707
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 1 cut(s) 1127
TaaI ACNGT 4 cut(s) 130, 853, 955, 1160
TaiI ACGT 3 cut(s) 165, 252, 343
TaqI TCGA 3 cut(s) 42, 111, 146
TatI WGTACW 1 cut(s) 955
TauI GCSGC 1 cut(s) 291
Tru1I TTAA 1 cut(s) 879
Tru9I TTAA 1 cut(s) 879
TscAI CASTG 1 cut(s) 856
TseI GCWGC 3 cut(s) 449, 903, 1021
TspDTI ATGAA 5 cut(s) 24, 663, 695, 963, 1215
TspGWI ACGGA 3 cut(s) 757, 798, 1092
TspRI CASTG 1 cut(s) 856
VpaK11BI GGWCC 2 cut(s) 997, 1052
XagI CCTNNNNNAGG 1 cut(s) 282
XceI RCATGY 2 cut(s) 449, 896
XspI CTAG 1 cut(s) 491
ZraI GACGTC 1 cut(s) 250
ZrmI AGTACT 1 cut(s) 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.