Rh6CG010200

WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005006
Physical Location & Seq
Forward (+)
1197986 .. 1201145
3160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG010200.1

Sequence Viewer

Length: 966 bp
ATGGCCGACGATCACGACCAAGAAGACACGGCCGTCGTTGAGGAAGAGTACGCCGTCTGGAAGAAGAACACTCCGTTCCTCTACGACCTCATCATCTCCCACCCTCTCGAATGGCCATCTCTCACCGTCCACTGGGTCCCCTCGGCCCCGCAGCCCTACTCCGATCCATCTCTCGCCGTCCACAAGCTCATCCTCGGGACCCACACCTCCGACGACTACCCCAACTACCTCATGGTCGCCGACGCCCTCCTCCCCGCATCTCAACCCAACGTTGACGCCGTTTCCGACGAGCCCACTCTCCCTAAGGTTGAGATTACGCAGAAGATTCGGGTCGATGGGGAAGTGAATAGGGCAAGGTGTATGCTGCAGAAGCCGAATATGGTTGGGGCGAAAACCAGCGGTAGCGAGGTGTATGTGTTTGATTGTACCAAGCAGGGGCTGAAGTCTCAAGATGGTGAGTGTGATCCTGATTTGAGGTTGAGGGGTCATGATAAAGAAGGGTATGGCTTGTCATGGAGTCCATTTAAGGAGGGATACCTTTTGAGTGGTTCAAATGATTGTAAAATTTGCTTGTGGGATGTGTCTGCTTTGGCTCAAGATAGAGTGCTTCATCCAATGCATGTTTTTGAGCGTCATGAAAGTGTGGTTAATGATGTATCATGGCATTCGAAGAACGAGAATCTGTTTGGATCTGTTGGGGACGATTGTCAATTGATGATATGGGACTTGAGAACAAATCAAGCCCAAGACTCTGTCAAAGTTCATGAGAAAGAGGTAAACTATCTATCTTTCCATCCGAGTAATGAGTGGGTTTTGGCTACAGCATCTTCAGACACTACTGTGGGTCTGTTTGATATGCGGAAGCTGACGGTACCATTGCATGCTCTGAGTGGCCACACGTATGCTCTTCATCCCTTGCTCCCCTCCTTGAAATTTATTCATATAACCGTCATCTCTGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0031461 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

321

Amino Acids

36.01

Weight (kDa)

5.14

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1C_H4-bd PF12265 15 - 85 1.3e-25 Histone-binding protein RBBP4 or subunit C of CAF1 complex
WD40_CDC20-Fz PF24807 111 - 253 2.7e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 127 - 285 1.2e-15 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 142 - 288 2.2e-09 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 144 - 303 5e-10 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 162 - 300 2.2e-17 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 163 - 260 3.3e-13 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 168 - 301 5.7e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_Aladin PF25460 169 - 278 8.3e-08 Aladin seven-bladed propeller
WD40_Prp19 PF24814 171 - 289 3.4e-15 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 178 - 288 2.3e-08 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 179 - 290 2.3e-09 WDR55
EIF3I PF24805 185 - 291 2e-08 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 207 - 288 4.8e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_EIPR1 PF23609 212 - 284 1e-08 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 212 - 298 4.3e-13 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 226 - 302 3.3e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
Acc65I GGTACC 1 cut(s) 871
AccB1I GGYRCC 1 cut(s) 871
AciI CCGC 4 cut(s) 149, 255, 399, 859
AclI AACGTT 1 cut(s) 270
AclWI GGATC 3 cut(s) 158, 458, 697
AcoI YGGCCR 4 cut(s) 3, 30, 113, 892
AcsI RAATTY 2 cut(s) 564, 932
AcuI CTGAAG 2 cut(s) 461, 813
AcyI GRCGYC 2 cut(s) 243, 276
AfaI GTAC 3 cut(s) 50, 427, 873
AfiI CCNNNNNNNGG 2 cut(s) 132, 435
AflIII ACRYGT 1 cut(s) 897
AgsI TTSAA 2 cut(s) 552, 931
AleI CACNNNNGTG 1 cut(s) 839
AluBI AGCT 2 cut(s) 187, 865
AluI AGCT 2 cut(s) 187, 865
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 3 cut(s) 158, 458, 697
AlwNI CAGNNNCTG 1 cut(s) 439
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 5 cut(s) 3, 30, 113, 144, 892
ApeKI GCWGC 2 cut(s) 151, 364
ApoI RAATTY 2 cut(s) 564, 932
Asp718I GGTACC 1 cut(s) 871
AspS9I GGNCC 3 cut(s) 136, 145, 198
AsuHPI GGTGA 2 cut(s) 115, 467
AsuII TTCGAA 1 cut(s) 668
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 2 cut(s) 136, 198
AxyI CCTNAGG 1 cut(s) 303
BalI TGGCCA 2 cut(s) 115, 894
BanI GGYRCC 1 cut(s) 871
BanII GRGCYC 1 cut(s) 294
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 163, 351
BccI CCATC 5 cut(s) 124, 175, 329, 446, 801
BceAI ACGGC 5 cut(s) 17, 38, 45, 161, 263
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciVI GTATCC 1 cut(s) 527
BcoDI GTCTC 1 cut(s) 450
BfmI CTRYAG 2 cut(s) 365, 819
BfuI GTATCC 1 cut(s) 527
BisI GCNGC 2 cut(s) 152, 365
BlsI GCNGC 2 cut(s) 153, 366
Bme18I GGWCC 2 cut(s) 136, 198
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 3 cut(s) 136, 145, 198
BmiI GGNNCC 6 cut(s) 137, 138, 147, 199, 200, 873
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 266, 833
BmuI ACTGGG 1 cut(s) 142
BoxI GACNNNNGTC 1 cut(s) 705
BpiI GAAGAC 1 cut(s) 30
Bpu14I TTCGAA 1 cut(s) 668
BpuEI CTTGAG 3 cut(s) 432, 579, 748
BsaAI YACGTR 1 cut(s) 900
BsaHI GRCGYC 2 cut(s) 243, 276
BsaJI CCNNGG 2 cut(s) 141, 193
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 435
Bse1I ACTGG 1 cut(s) 137
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 875
BseDI CCNNGG 2 cut(s) 141, 193
BseGI GGATG 5 cut(s) 189, 583, 610, 793, 910
BseLI CCNNNNNNNGG 2 cut(s) 132, 435
BseMI GCAATG 1 cut(s) 875
BseMII CTCAG 1 cut(s) 878
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 1 cut(s) 239
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 2 cut(s) 163, 351
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 5 cut(s) 5, 32, 115, 146, 894
BshNI GGYRCC 1 cut(s) 871
BsiEI CGRYCG 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 194
BslFI GGGAC 4 cut(s) 122, 211, 713, 737
BslI CCNNNNNNNGG 2 cut(s) 132, 435
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 4 cut(s) 122, 211, 713, 737
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 5 cut(s) 5, 32, 115, 146, 894
BsoBI CYCGRG 1 cut(s) 194
Bsp119I TTCGAA 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 4 cut(s) 10, 163, 463, 689
BspACI CCGC 4 cut(s) 149, 255, 399, 859
BspANI GGCC 5 cut(s) 5, 32, 115, 146, 894
BspCNI CTCAG 1 cut(s) 879
BspHI TCATGA 3 cut(s) 487, 634, 763
BspLI GGNNCC 6 cut(s) 137, 138, 147, 199, 200, 873
BspMAI CTGCAG 1 cut(s) 369
BspPI GGATC 3 cut(s) 158, 458, 697
BspQI GCTCTTC 1 cut(s) 912
BspT104I TTCGAA 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 871
BsrDI GCAATG 1 cut(s) 875
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 141, 193
BssMI GATC 4 cut(s) 10, 163, 463, 689
BssNI GRCGYC 2 cut(s) 243, 276
Bst4CI ACNGT 4 cut(s) 127, 841, 871, 949
Bst6I CTCTTC 2 cut(s) 39, 912
BstACI GRCGYC 2 cut(s) 243, 276
BstBAI YACGTR 1 cut(s) 900
BstBI TTCGAA 1 cut(s) 668
BstC8I GCNNGC 1 cut(s) 882
BstDEI CTNAG 2 cut(s) 303, 887
BstF5I GGATG 5 cut(s) 189, 583, 610, 793, 910
BstKTI GATC 4 cut(s) 13, 166, 466, 692
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 4 cut(s) 10, 163, 463, 689
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNSI RCATGY 2 cut(s) 623, 884
BstPAI GACNNNNGTC 1 cut(s) 705
BstSFI CTRYAG 2 cut(s) 365, 819
BstV1I GCAGC 2 cut(s) 163, 351
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 689
BstYI RGATCY 1 cut(s) 689
BstZI CGGCCG 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 303
BsuI GTATCC 1 cut(s) 527
BsuRI GGCC 5 cut(s) 5, 32, 115, 146, 894
BtsCI GGATG 5 cut(s) 189, 583, 610, 793, 910
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 1 cut(s) 882
CaiI CAGNNNCTG 1 cut(s) 439
CciI TCATGA 3 cut(s) 487, 634, 763
Cfr13I GGNCC 3 cut(s) 136, 145, 198
CseI GACGC 3 cut(s) 251, 284, 620
Csp6I GTAC 3 cut(s) 49, 426, 872
CviAII CATG 8 cut(s) 232, 488, 513, 620, 635, 660, 764, 881
CviQI GTAC 3 cut(s) 49, 426, 872
DdeI CTNAG 2 cut(s) 303, 887
DpnI GATC 4 cut(s) 12, 165, 465, 691
DpnII GATC 4 cut(s) 10, 163, 463, 689
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 4 cut(s) 3, 30, 113, 892
EagI CGGCCG 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 39, 912
EarI CTCTTC 2 cut(s) 39, 912
EclXI CGGCCG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 294
Eco47I GGWCC 2 cut(s) 136, 198
Eco52I CGGCCG 1 cut(s) 30
Eco57I CTGAAG 2 cut(s) 461, 813
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 194
EcoO109I RGGNCCY 2 cut(s) 136, 198
EcoT22I ATGCAT 1 cut(s) 621
EcoT38I GRGCYC 1 cut(s) 294
FaeI CATG 8 cut(s) 235, 491, 516, 623, 638, 663, 767, 884
FaqI GGGAC 4 cut(s) 122, 211, 713, 737
FatI CATG 8 cut(s) 231, 487, 512, 619, 634, 659, 763, 880
FauI CCCGC 2 cut(s) 156, 262
Fnu4HI GCNGC 2 cut(s) 152, 365
FokI GGATG 5 cut(s) 176, 590, 597, 780, 897
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 2 cut(s) 152, 365
GluI GCNGC 2 cut(s) 152, 365
HaeIII GGCC 5 cut(s) 5, 32, 115, 146, 894
HgaI GACGC 3 cut(s) 251, 284, 620
Hin1I GRCGYC 2 cut(s) 243, 276
Hin1II CATG 8 cut(s) 235, 491, 516, 623, 638, 663, 767, 884
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 4 cut(s) 325, 517, 679, 749
HphI GGTGA 2 cut(s) 115, 467
Hpy166II GTNNAC 4 cut(s) 130, 181, 274, 778
Hpy188I TCNGA 6 cut(s) 163, 211, 286, 798, 832, 888
Hpy8I GTNNAC 4 cut(s) 130, 181, 274, 778
Hpy99I CGWCG 5 cut(s) 11, 38, 215, 245, 290
HpyAV CCTTC 1 cut(s) 491
HpyCH4III ACNGT 4 cut(s) 127, 841, 871, 949
HpyCH4IV ACGT 2 cut(s) 270, 899
HpyCH4V TGCA 3 cut(s) 367, 619, 880
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpyF3I CTNAG 2 cut(s) 303, 887
HpySE526I ACGT 2 cut(s) 270, 899
Hsp92I GRCGYC 2 cut(s) 243, 276
Hsp92II CATG 8 cut(s) 235, 491, 516, 623, 638, 663, 767, 884
KflI GGGWCCC 2 cut(s) 136, 198
KpnI GGTACC 1 cut(s) 875
Kzo9I GATC 4 cut(s) 10, 163, 463, 689
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 1 cut(s) 924
LpnPI CCDG 5 cut(s) 43, 118, 409, 419, 480
Lsp1109I GCAGC 2 cut(s) 163, 351
LweI GCATC 2 cut(s) 266, 833
MaeII ACGT 2 cut(s) 270, 899
MalI GATC 4 cut(s) 12, 165, 465, 691
MboI GATC 4 cut(s) 10, 163, 463, 689
MboII GAAGA 8 cut(s) 35, 56, 73, 76, 334, 682, 819, 899
MfeI CAATTG 1 cut(s) 710
MflI RGATCY 1 cut(s) 689
MhlI GDGCHC 1 cut(s) 294
MlsI TGGCCA 2 cut(s) 115, 894
MluCI AATT 3 cut(s) 564, 710, 932
MluNI TGGCCA 2 cut(s) 115, 894
MlyI GAGTC 2 cut(s) 526, 743
MmeI TCCRAC 2 cut(s) 234, 309
Mox20I TGGCCA 2 cut(s) 115, 894
Mph1103I ATGCAT 1 cut(s) 621
MscI TGGCCA 2 cut(s) 115, 894
MseI TTAA 3 cut(s) 525, 648, 964
MslI CAYNNNNRTG 3 cut(s) 639, 839, 900
Msp20I TGGCCA 2 cut(s) 115, 894
MspA1I CMGCKG 1 cut(s) 399
MunI CAATTG 1 cut(s) 710
Mva1269I GAATGC 1 cut(s) 664
MwoI GCNNNNNNNGC 1 cut(s) 370
NdeII GATC 4 cut(s) 10, 163, 463, 689
NlaIII CATG 8 cut(s) 235, 491, 516, 623, 638, 663, 767, 884
NlaIV GGNNCC 6 cut(s) 137, 138, 147, 199, 200, 873
NmeAIII GCCGAG 1 cut(s) 122
NsiI ATGCAT 1 cut(s) 621
NspI RCATGY 2 cut(s) 623, 884
NspV TTCGAA 1 cut(s) 668
OliI CACNNNNGTG 1 cut(s) 839
PaeI GCATGC 1 cut(s) 884
PagI TCATGA 3 cut(s) 487, 634, 763
PciSI GCTCTTC 1 cut(s) 912
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 282
PctI GAATGC 1 cut(s) 664
PfeI GAWTC 2 cut(s) 325, 679
PflFI GACNNNGTC 1 cut(s) 752
PkrI GCNGC 2 cut(s) 153, 366
PleI GAGTC 2 cut(s) 525, 743
PpsI GAGTC 2 cut(s) 525, 743
Ppu21I YACGTR 1 cut(s) 900
PpuMI RGGWCCY 2 cut(s) 136, 198
PshAI GACNNNNGTC 1 cut(s) 705
Psp1406I AACGTT 1 cut(s) 270
Psp5II RGGWCCY 2 cut(s) 136, 198
PspN4I GGNNCC 6 cut(s) 137, 138, 147, 199, 200, 873
PspPI GGNCC 3 cut(s) 136, 145, 198
PspPPI RGGWCCY 2 cut(s) 136, 198
PstI CTGCAG 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 439
PsuI RGATCY 1 cut(s) 689
PsyI GACNNNGTC 1 cut(s) 752
RsaI GTAC 3 cut(s) 50, 427, 873
RsaNI GTAC 3 cut(s) 49, 426, 872
RseI CAYNNNNRTG 3 cut(s) 639, 839, 900
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 3 cut(s) 525, 648, 964
SatI GCNGC 2 cut(s) 152, 365
Sau3AI GATC 4 cut(s) 10, 163, 463, 689
Sau96I GGNCC 3 cut(s) 136, 145, 198
SchI GAGTC 2 cut(s) 526, 743
SduI GDGCHC 1 cut(s) 294
SfaNI GCATC 2 cut(s) 266, 833
SfcI CTRYAG 2 cut(s) 365, 819
SfuI TTCGAA 1 cut(s) 668
SinI GGWCC 2 cut(s) 136, 198
SmiMI CAYNNNNRTG 3 cut(s) 639, 839, 900
SmlI CTYRAG 3 cut(s) 447, 594, 727
SmoI CTYRAG 3 cut(s) 447, 594, 727
SphI GCATGC 1 cut(s) 884
Sse9I AATT 3 cut(s) 564, 710, 932
SsiI CCGC 4 cut(s) 149, 255, 399, 859
TaaI ACNGT 4 cut(s) 127, 841, 871, 949
TaiI ACGT 2 cut(s) 273, 902
TaqI TCGA 3 cut(s) 108, 333, 668
TasI AATT 3 cut(s) 564, 710, 932
TfiI GAWTC 2 cut(s) 325, 679
Tru1I TTAA 3 cut(s) 525, 648, 964
Tru9I TTAA 3 cut(s) 525, 648, 964
TscAI CASTG 1 cut(s) 137
TseI GCWGC 2 cut(s) 151, 364
TspDTI ATGAA 5 cut(s) 599, 651, 752, 899, 929
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 1 cut(s) 137
Tth111I GACNNNGTC 1 cut(s) 752
VpaK11BI GGWCC 2 cut(s) 136, 198
XapI RAATTY 2 cut(s) 564, 932
XceI RCATGY 2 cut(s) 623, 884
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
Zsp2I ATGCAT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.