AT4G35050

WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005006
Physical Location & Seq
Reverse (-)
16682580 .. 16684928
2349 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35050.1

Sequence Viewer

Length: 1275 bp
ATGGCGGCAGAAGAGGGTAAAGATGAGGCGGGATTGGATCAGGTGGAAGAAGAGTTCTCTATTTGGAAAAGGAACACACCTTTTCTCTACGATCTTATGATCTCTCACCCACTAGAATGGCCTTCTCTTACTCTCCACTGGGTTCCTTCGACGCCGATTCCGTACTCGAAAGATCCTTACTTTGCCGTCCATAAGCTTATCCTTGGGACTCATACCTCCGGTGGTGCCCAAGATTTTCTAATGGTCGCCGATGTCGTTATTCCGACACCCGACGCTGAACCAGGATTAGGTGGAAGAGATCAGGAACCTATCGTCCCTAAGGTGGAGATTAAGCAGAAGATACGTGTTGATGGAGAAGTGAACAGAGCGAGGTGTATGCCACAAAAGCCAACTCTTGTGGGTGCTAAAACAAGTGGCTCTGAGGTGTTTCTGTTTGATTACGCCAGACTCTCTGGAAAGCCCCAAACAAGTGAGTGTGATCCTGATCTGAGGCTAATGGGACATGAACAAGAAGGTTATGGATTGGCTTGGAGTTCCTTTAAGGAGGGTTATCTTTTGAGTGGCTCACAAGATCAGAGAATCTGCCTTTGGGATGTCTCAGCGACTGCCACTGATAAAGTTTTGAACCCAATGCATGTGTATGAGGGACATCAAAGTATCATCGAAGATGTAGCATGGCACATGAAGAATGAAAACATATTCGGGTCTGCGGGTGATGATTGTCAATTGGTGATATGGGACTTACGGACTAACCAAATGCAACACCAAGTCAAAGTTCACGAGAGAGAGATAAACTATCTTTCTTTCAATCCCTTCAATGAATGGGTACTAGCCACAGCTTCCTCAGACTCAACTGTCGCCTTGTTTGACCTTCGTAAGCTGACTGCGCCATTACATGTCCTGAGCAAACACGAGGGAGAAGTTTTCCAAGTGGAGTGGGATCCCAACCATGAAACAGTGCTTGCATCTTCTGGCGAGGACAGAAGACTCATGGTCTGGGATATCAACAGGGTTGGGGATGAGCAGCTTGAAATAGAGTTGGATGCAGAAGATGGTCCACCTGAGCTGCTCTTCTCTCATGGAGGCCACAAGGCCAAGATATCAGACTTTGCCTGGAACAAGGACGAGCCTTGGGTCATCTCAAGTGTAGCTGAAGACAACAGTCTTCAAGTCTGGCAAATGGCGGAAAGCATCTACCGCGAAGATGACGAGGATGAGGATGATGATGATGAAGGCAACCAAAATGCACAACATTCCAATGAAAACCAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0031461 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

424

Amino Acids

47.98

Weight (kDa)

4.55

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1C_H4-bd PF12265 17 - 88 2.3e-24 Histone-binding protein RBBP4 or subunit C of CAF1 complex
WDR55 PF24796 112 - 293 6.9e-12 WDR55
WD40_CDC20-Fz PF24807 118 - 258 9.4e-13 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 142 - 262 8.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 162 - 291 7.6e-16 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 163 - 270 8.6e-13 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 168 - 271 7.3e-17 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 168 - 292 9.6e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 174 - 338 8.3e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR19_1st PF23389 175 - 394 1.7e-07 WDR19 first beta-propeller
Beta-prop_EIPR1 PF23609 176 - 289 3.8e-13 EIPR1 beta-propeller
WD40_Prp19 PF24814 178 - 337 7.2e-19 Prp19 WD40 domain
WD40_Gbeta PF25391 185 - 293 1.2e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 186 - 337 5.9e-22 WDR5 beta-propeller domain
Beta-prop_EML PF23409 187 - 360 9.9e-08 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 190 - 295 9.4e-08 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 211 - 293 5.8e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 213 - 335 2e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 230 - 337 2.3e-15 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 231 - 396 7.5e-10 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 242 - 333 2e-08 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 260 - 337 1e-12 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 264 - 337 3.5e-06 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 301 - 396 4.3e-06 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 854
AccB1I GGYRCC 1 cut(s) 224
AccII CGCG 1 cut(s) 1200
AciI CCGC 5 cut(s) 5, 29, 710, 1184, 1198
AclWI GGATC 5 cut(s) 45, 167, 473, 935, 948
AcuI CTGAAG 1 cut(s) 1173
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 2 cut(s) 164, 828
AfiI CCNNNNNNNGG 2 cut(s) 287, 322
AflIII ACRYGT 2 cut(s) 343, 895
AgsI TTSAA 5 cut(s) 625, 808, 817, 1031, 1169
AhdI GACNNNNNGTC 1 cut(s) 992
AjnI CCWGG 2 cut(s) 280, 1112
AjuI GAANNNNNNNTTGG 4 cut(s) 571, 603, 759, 791
AloI GAACNNNNNNTCC 4 cut(s) 38, 70, 297, 329
AluBI AGCT 6 cut(s) 196, 839, 880, 1027, 1066, 1151
AluI AGCT 6 cut(s) 196, 839, 880, 1027, 1066, 1151
Alw26I GTCTC 1 cut(s) 601
AlwI GGATC 5 cut(s) 45, 167, 473, 935, 948
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 3 cut(s) 119, 1084, 1092
ApeKI GCWGC 2 cut(s) 1024, 1066
AspLEI GCGC 1 cut(s) 889
AspS9I GGNCC 1 cut(s) 1055
AsuHPI GGTGA 3 cut(s) 98, 725, 742
AvaII GGWCC 1 cut(s) 1055
AxyI CCTNAGG 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 229
BamHI GGATCC 1 cut(s) 940
BanI GGYRCC 1 cut(s) 224
BauI CACGAG 2 cut(s) 779, 911
BbsI GAAGAC 3 cut(s) 991, 1157, 1161
BbvI GCAGC 2 cut(s) 1036, 1053
BccI CCATC 2 cut(s) 344, 1046
BceAI ACGGC 1 cut(s) 170
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 2 cut(s) 282, 1114
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 2 cut(s) 113, 830
BisI GCNGC 3 cut(s) 6, 1025, 1067
BlsI GCNGC 3 cut(s) 7, 1026, 1068
Bme1390I CCNGG 2 cut(s) 282, 1114
Bme18I GGWCC 1 cut(s) 1055
BmeRI GACNNNNNGTC 1 cut(s) 992
BmgT120I GGNCC 1 cut(s) 1055
BmiI GGNNCC 4 cut(s) 144, 226, 306, 942
BmrFI CCNGG 2 cut(s) 282, 1114
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 3 cut(s) 974, 1033, 1200
BmuI ACTGGG 1 cut(s) 148
BoxI GACNNNNGTC 1 cut(s) 1161
BpiI GAAGAC 3 cut(s) 991, 1157, 1161
Bpu10I CCTNAGC 2 cut(s) 902, 1062
BpuEI CTTGAG 1 cut(s) 1126
BsaAI YACGTR 1 cut(s) 344
BsaBI GATNNNNATC 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 202, 1130
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 322
Bse1I ACTGG 1 cut(s) 143
Bse21I CCTNAGG 1 cut(s) 318
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 2 cut(s) 282, 1114
BseDI CCNNGG 2 cut(s) 202, 1130
BseGI GGATG 5 cut(s) 598, 1024, 1048, 1219, 1225
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 2 cut(s) 287, 322
BseMII CTCAG 6 cut(s) 411, 479, 612, 858, 893, 1053
BseNI ACTGG 1 cut(s) 143
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 2 cut(s) 1036, 1053
Bsh1236I CGCG 1 cut(s) 1200
BshFI GGCC 3 cut(s) 121, 1086, 1094
BshNI GGYRCC 1 cut(s) 224
BsiSI CCGG 1 cut(s) 219
BslFI GGGAC 5 cut(s) 220, 299, 513, 660, 752
BslI CCNNNNNNNGG 2 cut(s) 287, 322
BsmAI GTCTC 1 cut(s) 601
BsmFI GGGAC 5 cut(s) 220, 299, 513, 660, 752
BsnI GGCC 3 cut(s) 121, 1086, 1094
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 9 cut(s) 37, 91, 99, 172, 298, 478, 484, 571, 940
BspACI CCGC 5 cut(s) 5, 29, 710, 1184, 1198
BspANI GGCC 3 cut(s) 121, 1086, 1094
BspCNI CTCAG 6 cut(s) 412, 480, 611, 857, 894, 1054
BspFNI CGCG 1 cut(s) 1200
BspLI GGNNCC 4 cut(s) 144, 226, 306, 942
BspPI GGATC 5 cut(s) 45, 167, 473, 935, 948
BspQI GCTCTTC 1 cut(s) 1076
BspT107I GGYRCC 1 cut(s) 224
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 2 cut(s) 202, 1130
BssMI GATC 9 cut(s) 37, 91, 99, 172, 298, 478, 484, 571, 940
BssNI GRCGYC 1 cut(s) 152
BssSI CACGAG 2 cut(s) 779, 911
BssT1I CCWWGG 2 cut(s) 202, 1130
Bst2BI CACGAG 2 cut(s) 779, 911
Bst2UI CCWGG 2 cut(s) 282, 1114
Bst4CI ACNGT 3 cut(s) 856, 958, 1163
Bst6I CTCTTC 4 cut(s) 6, 45, 289, 1076
BstACI GRCGYC 1 cut(s) 152
BstBAI YACGTR 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 963
BstDEI CTNAG 7 cut(s) 318, 420, 488, 598, 844, 902, 1062
BstF5I GGATG 5 cut(s) 598, 1024, 1048, 1219, 1225
BstFNI CGCG 1 cut(s) 1200
BstHHI GCGC 1 cut(s) 889
BstKTI GATC 9 cut(s) 40, 94, 102, 175, 301, 481, 487, 574, 943
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 9 cut(s) 37, 91, 99, 172, 298, 478, 484, 571, 940
BstMWI GCNNNNNNNGC 3 cut(s) 385, 886, 1197
BstNI CCWGG 2 cut(s) 282, 1114
BstNSI RCATGY 2 cut(s) 638, 899
BstPAI GACNNNNGTC 1 cut(s) 1161
BstSCI CCNGG 2 cut(s) 280, 1112
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 1 cut(s) 1200
BstV1I GCAGC 2 cut(s) 1036, 1053
BstV2I GAAGAC 3 cut(s) 991, 1157, 1161
BstX2I RGATCY 2 cut(s) 172, 940
BstXI CCANNNNNNTGG 1 cut(s) 117
BstYI RGATCY 2 cut(s) 172, 940
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 3 cut(s) 121, 1086, 1094
BtsCI GGATG 5 cut(s) 598, 1024, 1048, 1219, 1225
BtsIMutI CAGTG 3 cut(s) 136, 609, 963
Cac8I GCNNGC 1 cut(s) 963
CaiI CAGNNNCTG 1 cut(s) 605
CfoI GCGC 1 cut(s) 889
Cfr13I GGNCC 1 cut(s) 1055
CseI GACGC 2 cut(s) 160, 281
Csp6I GTAC 2 cut(s) 163, 827
CspCI CAANNNNNGTGG 4 cut(s) 378, 413, 917, 952
CviAII CATG 8 cut(s) 503, 635, 675, 682, 896, 950, 991, 1079
CviQI GTAC 2 cut(s) 163, 827
DdeI CTNAG 7 cut(s) 318, 420, 488, 598, 844, 902, 1062
DpnI GATC 9 cut(s) 39, 93, 101, 174, 300, 480, 486, 573, 942
DpnII GATC 9 cut(s) 37, 91, 99, 172, 298, 478, 484, 571, 940
DrdI GACNNNNNNGTC 1 cut(s) 854
DriI GACNNNNNGTC 1 cut(s) 992
DseDI GACNNNNNNGTC 1 cut(s) 854
Eam1104I CTCTTC 4 cut(s) 6, 45, 289, 1076
Eam1105I GACNNNNNGTC 1 cut(s) 992
EarI CTCTTC 4 cut(s) 6, 45, 289, 1076
EciI GGCGGA 1 cut(s) 1199
Eco130I CCWWGG 2 cut(s) 202, 1130
Eco32I GATATC 2 cut(s) 1003, 1101
Eco47I GGWCC 1 cut(s) 1055
Eco57I CTGAAG 1 cut(s) 1173
Eco81I CCTNAGG 1 cut(s) 318
EcoRII CCWGG 2 cut(s) 280, 1112
EcoRV GATATC 2 cut(s) 1003, 1101
EcoT14I CCWWGG 2 cut(s) 202, 1130
EcoT22I ATGCAT 1 cut(s) 636
ErhI CCWWGG 2 cut(s) 202, 1130
FaeI CATG 8 cut(s) 506, 638, 678, 685, 899, 953, 994, 1082
FaqI GGGAC 5 cut(s) 220, 299, 513, 660, 752
FatI CATG 8 cut(s) 502, 634, 674, 681, 895, 949, 990, 1078
FauI CCCGC 2 cut(s) 22, 703
Fnu4HI GCNGC 3 cut(s) 6, 1025, 1067
FokI GGATG 5 cut(s) 605, 1031, 1055, 1226, 1232
Fsp4HI GCNGC 3 cut(s) 6, 1025, 1067
FspBI CTAG 2 cut(s) 113, 830
GlaI GCGC 1 cut(s) 888
GluI GCNGC 3 cut(s) 6, 1025, 1067
HaeIII GGCC 3 cut(s) 121, 1086, 1094
HapII CCGG 1 cut(s) 219
HgaI GACGC 2 cut(s) 160, 281
HhaI GCGC 1 cut(s) 889
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 8 cut(s) 506, 638, 678, 685, 899, 953, 994, 1082
Hin6I GCGC 1 cut(s) 887
HinP1I GCGC 1 cut(s) 887
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 6 cut(s) 157, 208, 447, 579, 848, 987
HpaII CCGG 1 cut(s) 219
HphI GGTGA 3 cut(s) 98, 725, 742
Hpy166II GTNNAC 3 cut(s) 361, 778, 1058
Hpy188I TCNGA 6 cut(s) 264, 421, 489, 576, 847, 1105
Hpy188III TCNNGA 5 cut(s) 302, 453, 482, 779, 901
Hpy8I GTNNAC 3 cut(s) 361, 778, 1058
Hpy99I CGWCG 2 cut(s) 154, 275
HpyAV CCTTC 6 cut(s) 132, 156, 506, 823, 881, 1226
HpyCH4III ACNGT 3 cut(s) 856, 958, 1163
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 5 cut(s) 634, 760, 965, 1046, 1247
HpyF10VI GCNNNNNNNGC 3 cut(s) 385, 886, 1197
HpyF3I CTNAG 7 cut(s) 318, 420, 488, 598, 844, 902, 1062
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 8 cut(s) 506, 638, 678, 685, 899, 953, 994, 1082
HspAI GCGC 1 cut(s) 887
Kzo9I GATC 9 cut(s) 37, 91, 99, 172, 298, 478, 484, 571, 940
LguI GCTCTTC 1 cut(s) 1076
Lsp1109I GCAGC 2 cut(s) 1036, 1053
LweI GCATC 3 cut(s) 974, 1033, 1200
MaeI CTAG 2 cut(s) 113, 830
MaeII ACGT 1 cut(s) 343
MalI GATC 9 cut(s) 39, 93, 101, 174, 300, 480, 486, 573, 942
MboI GATC 9 cut(s) 37, 91, 99, 172, 298, 478, 484, 571, 940
MfeI CAATTG 1 cut(s) 725
MflI RGATCY 2 cut(s) 172, 940
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 1 cut(s) 725
MlyI GAGTC 4 cut(s) 202, 441, 842, 981
MmeI TCCRAC 2 cut(s) 287, 1020
Mph1103I ATGCAT 1 cut(s) 636
MseI TTAA 2 cut(s) 330, 540
MslI CAYNNNNRTG 3 cut(s) 115, 639, 1257
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 282, 1114
MunI CAATTG 1 cut(s) 725
MvaI CCWGG 2 cut(s) 282, 1114
MvnI CGCG 1 cut(s) 1200
MwoI GCNNNNNNNGC 3 cut(s) 385, 886, 1197
NdeII GATC 9 cut(s) 37, 91, 99, 172, 298, 478, 484, 571, 940
NlaIII CATG 8 cut(s) 506, 638, 678, 685, 899, 953, 994, 1082
NlaIV GGNNCC 4 cut(s) 144, 226, 306, 942
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 2 cut(s) 638, 899
PciI ACATGT 1 cut(s) 895
PciSI GCTCTTC 1 cut(s) 1076
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 252
PfeI GAWTC 2 cut(s) 157, 579
PkrI GCNGC 3 cut(s) 7, 1026, 1068
PleI GAGTC 4 cut(s) 202, 441, 842, 981
PpsI GAGTC 4 cut(s) 202, 441, 842, 981
Ppu21I YACGTR 1 cut(s) 344
PscI ACATGT 1 cut(s) 895
PshAI GACNNNNGTC 1 cut(s) 1161
Psp6I CCWGG 2 cut(s) 280, 1112
PspGI CCWGG 2 cut(s) 280, 1112
PspN4I GGNNCC 4 cut(s) 144, 226, 306, 942
PspPI GGNCC 1 cut(s) 1055
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 2 cut(s) 172, 940
RsaI GTAC 2 cut(s) 164, 828
RsaNI GTAC 2 cut(s) 163, 827
RseI CAYNNNNRTG 3 cut(s) 115, 639, 1257
SapI GCTCTTC 1 cut(s) 1076
SaqAI TTAA 2 cut(s) 330, 540
SatI GCNGC 3 cut(s) 6, 1025, 1067
Sau3AI GATC 9 cut(s) 37, 91, 99, 172, 298, 478, 484, 571, 940
Sau96I GGNCC 1 cut(s) 1055
SchI GAGTC 4 cut(s) 202, 441, 842, 981
ScrFI CCNGG 2 cut(s) 282, 1114
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 3 cut(s) 974, 1033, 1200
SinI GGWCC 1 cut(s) 1055
SmiMI CAYNNNNRTG 3 cut(s) 115, 639, 1257
SmlI CTYRAG 1 cut(s) 1141
SmoI CTYRAG 1 cut(s) 1141
Sse9I AATT 1 cut(s) 725
SsiI CCGC 5 cut(s) 5, 29, 710, 1184, 1198
SspMI CTAG 2 cut(s) 113, 830
StyD4I CCNGG 2 cut(s) 280, 1112
StyI CCWWGG 2 cut(s) 202, 1130
TaaI ACNGT 3 cut(s) 856, 958, 1163
TaiI ACGT 1 cut(s) 346
TaqI TCGA 3 cut(s) 149, 167, 663
TasI AATT 1 cut(s) 725
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 157, 579
Tru1I TTAA 2 cut(s) 330, 540
Tru9I TTAA 2 cut(s) 330, 540
TscAI CASTG 3 cut(s) 143, 616, 963
TseI GCWGC 2 cut(s) 1024, 1066
TspDTI ATGAA 7 cut(s) 519, 698, 705, 834, 966, 1245, 1275
TspGWI ACGGA 2 cut(s) 150, 760
TspRI CASTG 3 cut(s) 143, 616, 963
VpaK11BI GGWCC 1 cut(s) 1055
XceI RCATGY 2 cut(s) 638, 899
XspI CTAG 2 cut(s) 113, 830
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.