MD10G1124300.v1.1

WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005006
Physical Location & Seq
Reverse (-)
20295421 .. 20298093
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1124300.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGCGGAAGACCAGGACCAAGAATCGGCTGTCTTAGAGGAAGAGTACACGGTCTGGAAGAAGAACAGCCCTTTCCTCTACGACCTCATCATCTCTCACCCTCTCGAATGGCCGTCTCTCACTGTCCACTGGGCTCCCTCGGCGCCGCAGCCCCACACCGACCCATCTCTCGCCGCCCACAAGCTCGTTCTCGGGACCCTCACTTCCGATGACTACCCCAACTTTCTCATGGTCGCCGACGCCCTCCTACCCACATGCCAACCCAACTTTGGCGCCATTTCGGAAAATGAACCTGTGATGCCTAAGGTGGAAATAACGCAGAAGATTCGTGTCTTTGGGGAAGTGAATAGGGCACGGTGGATGCCGCAGAAGTCGACTATTGTGGGTGCAAAGACCAGCGGTGCTGATGTTTATGTGCTTGATTGTTCCAAACAAGGAGGAAAGAAACATCAAGATGAGGCCTGTCATCCTGATTTGAGGTTAAGGGGCCATGACAAAGAAGGTGTGGTTGGAGATGTTTCATGGCATCCGAAGAATGAGAATCTGTTTGGATCCGTTGGGGATGATTGTCAGTTGATGATATGGGACTTGCGCACTAACCAAACACAGCATTGTGTCAAAACTCATGAGACGGAGATAAACTCTCTTTCTTTCAATTCATATAATGAATGGATTTTGGCCACAGCATCTTCAGACACCACTGTTGGTCTGTTCGATATGAGAAGGCTGACCATTCCATTGCATGTTCTGAGTAGCCATGGGGAAGAGGTGTTCCAGGTTGAATGTGATCCTAATCATGAAACCGTATTGGCATCCTTCGCTGACAACAGGAGGTTGATGGTTTGGGACCTCAACAGGGTTGGGGAGGAGCGGGCAGAGGGAGATGGCGATGATGGACCGCCAGAACTTCCCTTCGCACACGGCGGTCACAAAGCAAAGATATCAGATCTCTCGTGGAACAAAAACGAGCCATGGGTCATATCAAGTGTTGCTGAAGACAAAACTCTTGAGGTTTGGCAGATTGCTGACAGCATATTTGGCGACGACGATGTTGTGCAAGTCCAGCCTTCCAATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0031461 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

360

Amino Acids

40.17

Weight (kDa)

4.76

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1C_H4-bd PF12265 14 - 84 5.4e-23 Histone-binding protein RBBP4 or subunit C of CAF1 complex
WDR55 PF24796 111 - 285 2.8e-09 WDR55
Beta-prop_EIPR1 PF23609 114 - 238 1.7e-10 EIPR1 beta-propeller
WD40_Prp19 PF24814 124 - 264 7.5e-12 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 134 - 342 3.2e-16 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 147 - 242 6.7e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 148 - 288 3e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 155 - 219 6.4e-09 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 159 - 284 1.3e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 160 - 285 2e-15 WDR5 beta-propeller domain
WD40_Gbeta PF25391 170 - 242 5.5e-06 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 173 - 283 1.3e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 175 - 283 3.1e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 190 - 277 2.1e-07 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 209 - 286 5.5e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 593
AccB1I GGYRCC 2 cut(s) 142, 272
AccBSI CCGCTC 1 cut(s) 871
AccI GTMKAC 1 cut(s) 374
AciI CCGC 8 cut(s) 5, 146, 174, 365, 399, 871, 899, 924
AclWI GGATC 3 cut(s) 546, 559, 782
AcoI YGGCCR 2 cut(s) 110, 678
AcuI CTGAAG 2 cut(s) 675, 1014
AcyI GRCGYC 3 cut(s) 143, 240, 273
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 3 cut(s) 25, 269, 856
AgsI TTSAA 2 cut(s) 655, 782
AjnI CCWGG 2 cut(s) 12, 774
AjuI GAANNNNNNNTTGG 2 cut(s) 492, 524
AloI GAACNNNNNNTCC 2 cut(s) 755, 787
AluBI AGCT 1 cut(s) 184
AluI AGCT 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 120, 623
AlwI GGATC 3 cut(s) 546, 559, 782
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 4 cut(s) 110, 459, 487, 678
ApeKI GCWGC 1 cut(s) 148
ArsI GACNNNNNNTTYG 2 cut(s) 1019, 1051
AspLEI GCGC 3 cut(s) 145, 275, 594
AspS9I GGNCC 5 cut(s) 16, 195, 487, 847, 896
AsuHPI GGTGA 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 4 cut(s) 16, 195, 847, 896
AxyI CCTNAGG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 355
BalI TGGCCA 1 cut(s) 680
BamHI GGATCC 1 cut(s) 551
BanI GGYRCC 2 cut(s) 142, 272
BanII GRGCYC 1 cut(s) 136
BauI CACGAG 1 cut(s) 952
BbsI GAAGAC 2 cut(s) 15, 1002
BbvI GCAGC 1 cut(s) 160
BccI CCATC 4 cut(s) 172, 832, 878, 887
BceAI ACGGC 2 cut(s) 97, 937
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 2 cut(s) 14, 776
BcoDI GTCTC 2 cut(s) 120, 623
BfoI RGCGCY 2 cut(s) 146, 276
BglII AGATCT 1 cut(s) 946
BisI GCNGC 4 cut(s) 146, 149, 174, 365
BlsI GCNGC 4 cut(s) 147, 150, 175, 366
Bme1390I CCNGG 2 cut(s) 14, 776
Bme18I GGWCC 4 cut(s) 16, 195, 847, 896
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 5 cut(s) 16, 195, 487, 847, 896
BmiI GGNNCC 8 cut(s) 135, 144, 196, 197, 274, 488, 553, 848
BmrFI CCNGG 2 cut(s) 14, 776
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 5 cut(s) 288, 351, 535, 695, 821
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 15, 1002
BplI GAGNNNNNCTC 2 cut(s) 626, 658
BpuEI CTTGAG 1 cut(s) 1028
BsaBI GATNNNNATC 1 cut(s) 792
BsaHI GRCGYC 3 cut(s) 143, 240, 273
BsaJI CCNNGG 3 cut(s) 138, 757, 971
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 269, 856
Bse1I ACTGG 1 cut(s) 134
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 737
Bse8I GATNNNNATC 1 cut(s) 792
BseBI CCWGG 2 cut(s) 14, 776
BseDI CCNNGG 3 cut(s) 138, 757, 971
BseGI GGATG 5 cut(s) 366, 466, 526, 568, 812
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 3 cut(s) 25, 269, 856
BseMI GCAATG 1 cut(s) 737
BseMII CTCAG 1 cut(s) 740
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 881
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 1 cut(s) 160
BshFI GGCC 4 cut(s) 112, 461, 489, 680
BshNI GGYRCC 2 cut(s) 142, 272
BsiHKCI CYCGRG 1 cut(s) 191
BslFI GGGAC 3 cut(s) 208, 599, 860
BslI CCNNNNNNNGG 3 cut(s) 25, 269, 856
BsmAI GTCTC 2 cut(s) 120, 623
BsmBI CGTCTC 2 cut(s) 120, 623
BsmFI GGGAC 3 cut(s) 208, 599, 860
BsnI GGCC 4 cut(s) 112, 461, 489, 680
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 2 cut(s) 136, 355
Bsp143I GATC 3 cut(s) 551, 787, 946
Bsp19I CCATGG 2 cut(s) 757, 971
BspACI CCGC 8 cut(s) 5, 146, 174, 365, 399, 871, 899, 924
BspANI GGCC 4 cut(s) 112, 461, 489, 680
BspCNI CTCAG 1 cut(s) 741
BspHI TCATGA 2 cut(s) 625, 796
BspLI GGNNCC 8 cut(s) 135, 144, 196, 197, 274, 488, 553, 848
BspPI GGATC 3 cut(s) 546, 559, 782
BspT107I GGYRCC 2 cut(s) 142, 272
BsrBI CCGCTC 1 cut(s) 871
BsrDI GCAATG 1 cut(s) 737
BsrI ACTGG 1 cut(s) 134
BssECI CCNNGG 3 cut(s) 138, 757, 971
BssMI GATC 3 cut(s) 551, 787, 946
BssNI GRCGYC 3 cut(s) 143, 240, 273
BssSI CACGAG 1 cut(s) 952
BssT1I CCWWGG 2 cut(s) 757, 971
Bst2BI CACGAG 1 cut(s) 952
Bst2UI CCWGG 2 cut(s) 14, 776
Bst4CI ACNGT 5 cut(s) 52, 124, 357, 703, 805
Bst6I CTCTTC 2 cut(s) 36, 759
BstACI GRCGYC 3 cut(s) 143, 240, 273
BstC8I GCNNGC 1 cut(s) 873
BstDEI CTNAG 3 cut(s) 34, 303, 749
BstDSI CCRYGG 2 cut(s) 757, 971
BstF5I GGATG 5 cut(s) 366, 466, 526, 568, 812
BstH2I RGCGCY 2 cut(s) 146, 276
BstHHI GCGC 3 cut(s) 145, 275, 594
BstKTI GATC 3 cut(s) 554, 790, 949
BstMAI GTCTC 2 cut(s) 120, 623
BstMBI GATC 3 cut(s) 551, 787, 946
BstMWI GCNNNNNNNGC 4 cut(s) 140, 818, 1038, 1063
BstNI CCWGG 2 cut(s) 14, 776
BstNSI RCATGY 2 cut(s) 258, 746
BstSCI CCNGG 2 cut(s) 12, 774
BstSLI GKGCMC 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 160
BstV2I GAAGAC 2 cut(s) 15, 1002
BstX2I RGATCY 2 cut(s) 551, 946
BstYI RGATCY 2 cut(s) 551, 946
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 4 cut(s) 112, 461, 489, 680
BtgI CCRYGG 2 cut(s) 757, 971
BtgZI GCGATG 1 cut(s) 903
BtsCI GGATG 5 cut(s) 366, 466, 526, 568, 812
BtsIMutI CAGTG 3 cut(s) 120, 127, 699
Cac8I GCNNGC 1 cut(s) 873
CciI TCATGA 2 cut(s) 625, 796
CfoI GCGC 3 cut(s) 145, 275, 594
Cfr13I GGNCC 5 cut(s) 16, 195, 487, 847, 896
CseI GACGC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 46
CviAII CATG 9 cut(s) 229, 255, 491, 522, 626, 743, 758, 797, 972
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 3 cut(s) 34, 303, 749
DinI GGCGCC 2 cut(s) 144, 274
DpnI GATC 3 cut(s) 553, 789, 948
DpnII GATC 3 cut(s) 551, 787, 946
EaeI YGGCCR 2 cut(s) 110, 678
Eam1104I CTCTTC 2 cut(s) 36, 759
EarI CTCTTC 2 cut(s) 36, 759
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 757, 971
Eco147I AGGCCT 1 cut(s) 461
Eco24I GRGCYC 1 cut(s) 136
Eco32I GATATC 1 cut(s) 942
Eco47I GGWCC 4 cut(s) 16, 195, 847, 896
Eco57I CTGAAG 2 cut(s) 675, 1014
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 191
EcoO109I RGGNCCY 2 cut(s) 195, 847
EcoRII CCWGG 2 cut(s) 12, 774
EcoRV GATATC 1 cut(s) 942
EcoT14I CCWWGG 2 cut(s) 757, 971
EcoT38I GRGCYC 1 cut(s) 136
EgeI GGCGCC 2 cut(s) 144, 274
EheI GGCGCC 2 cut(s) 144, 274
ErhI CCWWGG 2 cut(s) 757, 971
Esp3I CGTCTC 2 cut(s) 120, 623
FaeI CATG 9 cut(s) 232, 258, 494, 525, 629, 746, 761, 800, 975
FaqI GGGAC 3 cut(s) 208, 599, 860
FatI CATG 9 cut(s) 228, 254, 490, 521, 625, 742, 757, 796, 971
FauI CCCGC 1 cut(s) 864
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 4 cut(s) 146, 149, 174, 365
FokI GGATG 5 cut(s) 373, 453, 513, 575, 799
FriOI GRGCYC 1 cut(s) 136
Fsp4HI GCNGC 4 cut(s) 146, 149, 174, 365
FspI TGCGCA 1 cut(s) 593
GlaI GCGC 3 cut(s) 144, 274, 593
GluI GCNGC 4 cut(s) 146, 149, 174, 365
HaeII RGCGCY 2 cut(s) 146, 276
HaeIII GGCC 4 cut(s) 112, 461, 489, 680
HgaI GACGC 1 cut(s) 248
HhaI GCGC 3 cut(s) 145, 275, 594
Hin1I GRCGYC 3 cut(s) 143, 240, 273
Hin1II CATG 9 cut(s) 232, 258, 494, 525, 629, 746, 761, 800, 975
Hin6I GCGC 3 cut(s) 143, 273, 592
HinP1I GCGC 3 cut(s) 143, 273, 592
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 3 cut(s) 23, 325, 541
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 3 cut(s) 48, 127, 375
Hpy188I TCNGA 6 cut(s) 208, 283, 531, 694, 750, 946
Hpy188III TCNNGA 8 cut(s) 55, 104, 193, 452, 470, 626, 797, 1007
Hpy8I GTNNAC 3 cut(s) 48, 127, 375
Hpy99I CGWCG 3 cut(s) 242, 1046, 1049
HpyAV CCTTC 5 cut(s) 494, 717, 826, 922, 1077
HpyCH4III ACNGT 5 cut(s) 52, 124, 357, 703, 805
HpyCH4V TGCA 3 cut(s) 389, 742, 1057
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 818, 1038, 1063
HpyF3I CTNAG 3 cut(s) 34, 303, 749
Hsp92I GRCGYC 3 cut(s) 143, 240, 273
Hsp92II CATG 9 cut(s) 232, 258, 494, 525, 629, 746, 761, 800, 975
HspAI GCGC 3 cut(s) 143, 273, 592
KasI GGCGCC 2 cut(s) 142, 272
KflI GGGWCCC 1 cut(s) 195
Kzo9I GATC 3 cut(s) 551, 787, 946
LmnI GCTCC 2 cut(s) 139, 868
Lsp1109I GCAGC 1 cut(s) 160
LweI GCATC 5 cut(s) 288, 351, 535, 695, 821
MaeIII GTNAC 1 cut(s) 926
MalI GATC 3 cut(s) 553, 789, 948
MbiI CCGCTC 1 cut(s) 871
MboI GATC 3 cut(s) 551, 787, 946
MboII GAAGA 9 cut(s) 20, 53, 70, 73, 334, 544, 681, 776, 1007
MflI RGATCY 2 cut(s) 551, 946
MhlI GDGCHC 2 cut(s) 136, 355
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 1 cut(s) 655
MluNI TGGCCA 1 cut(s) 680
Mly113I GGCGCC 2 cut(s) 143, 273
MmeI TCCRAC 1 cut(s) 490
Mox20I TGGCCA 1 cut(s) 680
MscI TGGCCA 1 cut(s) 680
MseI TTAA 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 453
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 399
MspR9I CCNGG 2 cut(s) 14, 776
MvaI CCWGG 2 cut(s) 14, 776
MwoI GCNNNNNNNGC 4 cut(s) 140, 818, 1038, 1063
NarI GGCGCC 2 cut(s) 143, 273
NcoI CCATGG 2 cut(s) 757, 971
NdeII GATC 3 cut(s) 551, 787, 946
NlaIII CATG 9 cut(s) 232, 258, 494, 525, 629, 746, 761, 800, 975
NlaIV GGNNCC 8 cut(s) 135, 144, 196, 197, 274, 488, 553, 848
NmeAIII GCCGAG 1 cut(s) 119
NmuCI GTSAC 1 cut(s) 926
NsbI TGCGCA 1 cut(s) 593
NspI RCATGY 2 cut(s) 258, 746
PagI TCATGA 2 cut(s) 625, 796
PceI AGGCCT 1 cut(s) 461
PfeI GAWTC 3 cut(s) 23, 325, 541
PkrI GCNGC 4 cut(s) 147, 150, 175, 366
PluTI GGCGCC 2 cut(s) 146, 276
PpuMI RGGWCCY 2 cut(s) 195, 847
Psp5II RGGWCCY 2 cut(s) 195, 847
Psp6I CCWGG 2 cut(s) 12, 774
PspGI CCWGG 2 cut(s) 12, 774
PspN4I GGNNCC 8 cut(s) 135, 144, 196, 197, 274, 488, 553, 848
PspPI GGNCC 5 cut(s) 16, 195, 487, 847, 896
PspPPI RGGWCCY 2 cut(s) 195, 847
PsuI RGATCY 2 cut(s) 551, 946
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 453
SalI GTCGAC 1 cut(s) 373
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 4 cut(s) 146, 149, 174, 365
Sau3AI GATC 3 cut(s) 551, 787, 946
Sau96I GGNCC 5 cut(s) 16, 195, 487, 847, 896
ScrFI CCNGG 2 cut(s) 14, 776
SduI GDGCHC 2 cut(s) 136, 355
SfaNI GCATC 5 cut(s) 288, 351, 535, 695, 821
SfoI GGCGCC 2 cut(s) 144, 274
SinI GGWCC 4 cut(s) 16, 195, 847, 896
SmiMI CAYNNNNRTG 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 1007
SmoI CTYRAG 1 cut(s) 1007
Sse9I AATT 1 cut(s) 655
SseBI AGGCCT 1 cut(s) 461
SsiI CCGC 8 cut(s) 5, 146, 174, 365, 399, 871, 899, 924
SspDI GGCGCC 2 cut(s) 142, 272
StuI AGGCCT 1 cut(s) 461
StyD4I CCNGG 2 cut(s) 12, 774
StyI CCWWGG 2 cut(s) 757, 971
TaaI ACNGT 5 cut(s) 52, 124, 357, 703, 805
TaqI TCGA 3 cut(s) 105, 374, 714
TasI AATT 1 cut(s) 655
TatI WGTACW 1 cut(s) 45
TauI GCSGC 3 cut(s) 148, 176, 367
TfiI GAWTC 3 cut(s) 23, 325, 541
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 3 cut(s) 127, 134, 706
TseFI GTSAC 1 cut(s) 926
TseI GCWGC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 926
TspDTI ATGAA 5 cut(s) 303, 510, 648, 681, 813
TspGWI ACGGA 2 cut(s) 544, 647
TspRI CASTG 3 cut(s) 127, 134, 706
VpaK11BI GGWCC 4 cut(s) 16, 195, 847, 896
XceI RCATGY 2 cut(s) 258, 746
XcmI CCANNNNNNNNNTGG 2 cut(s) 226, 266
XmiI GTMKAC 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.