AT2G20820

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004468
Physical Location & Seq
Forward (+)
8964199 .. 8965568
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20820.1

Sequence Viewer

Length: 282 bp
ATGGCGTCCTTCGCTCGCCGTGCCATGAGCTTAGCTCAGATCCCATCTGCTCGTATTTCCTCTGGAGCCGCTTCTATTGGTCAGCGTCGTTGCCTCGCCGGCGCCGCTGATCACCATGGATCTACCAAGGTTGACTTCTGGAAACAGCCCACGAACCCAGGAAACTGGAAAGAGGAGCATTTTGTGCTTATTTCTCTGTCTGGCTGGGGCTTACTTTTCTACAGTGGATACAAGCTCGCCACTGGGGGAAAAAAAGAGACGCCTGTGGAGAGTACACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

10.05

Weight (kDa)

9.8

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 101
AciI CCGC 2 cut(s) 69, 105
AclWI GGATC 2 cut(s) 34, 127
AcyI GRCGYC 3 cut(s) 5, 102, 260
AfaI GTAC 1 cut(s) 274
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 3 cut(s) 30, 35, 235
AluI AGCT 3 cut(s) 30, 35, 235
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 2 cut(s) 34, 127
AspLEI GCGC 1 cut(s) 104
AsuHPI GGTGA 1 cut(s) 104
BanI GGYRCC 1 cut(s) 101
BccI CCATC 1 cut(s) 52
BceAI ACGGC 1 cut(s) 3
BciT130I CCWGG 1 cut(s) 159
BciVI GTATCC 1 cut(s) 221
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 220
BfoI RGCGCY 1 cut(s) 105
BfuI GTATCC 1 cut(s) 221
BglI GCCNNNNNGGC 1 cut(s) 99
BisI GCNGC 2 cut(s) 69, 105
BlpI GCTNAGC 1 cut(s) 31
BlsI GCNGC 2 cut(s) 70, 106
Bme1390I CCNGG 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 67, 103
BmrFI CCNGG 1 cut(s) 159
BmrI ACTGGG 1 cut(s) 252
BmuI ACTGGG 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 19, 51
BpmI CTGGAG 1 cut(s) 84
Bpu1102I GCTNAGC 1 cut(s) 31
BsaHI GRCGYC 3 cut(s) 5, 102, 260
BsaJI CCNNGG 3 cut(s) 115, 126, 157
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 170, 247
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 115, 126, 157
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 2 cut(s) 170, 247
BseRI GAGGAG 1 cut(s) 188
BseYI CCCAGC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 101
BsiSI CCGG 1 cut(s) 99
BsmAI GTCTC 1 cut(s) 251
BsmBI CGTCTC 1 cut(s) 251
Bsp143I GATC 3 cut(s) 39, 109, 119
Bsp1720I GCTNAGC 1 cut(s) 31
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 2 cut(s) 69, 105
BspCNI CTCAG 1 cut(s) 49
BspLI GGNNCC 2 cut(s) 67, 103
BspPI GGATC 2 cut(s) 34, 127
BspT107I GGYRCC 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 2 cut(s) 170, 247
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 3 cut(s) 115, 126, 157
BssMI GATC 3 cut(s) 39, 109, 119
BssNI GRCGYC 3 cut(s) 5, 102, 260
BssT1I CCWWGG 2 cut(s) 115, 126
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 224
BstACI GRCGYC 3 cut(s) 5, 102, 260
BstAPI GCANNNNNTGC 1 cut(s) 184
BstC8I GCNNGC 3 cut(s) 16, 100, 237
BstDEI CTNAG 2 cut(s) 31, 36
BstDSI CCRYGG 1 cut(s) 115
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 3 cut(s) 42, 112, 122
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 3 cut(s) 39, 109, 119
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 99, 104, 184
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 220
BstX2I RGATCY 2 cut(s) 39, 119
BstXI CCANNNNNNTGG 1 cut(s) 165
BstYI RGATCY 2 cut(s) 39, 119
BsuI GTATCC 1 cut(s) 221
BtgI CCRYGG 1 cut(s) 115
BtsIMutI CAGTG 2 cut(s) 229, 240
Cac8I GCNNGC 3 cut(s) 16, 100, 237
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 98
CseI GACGC 2 cut(s) 74, 268
Csp6I GTAC 1 cut(s) 273
CviAII CATG 2 cut(s) 25, 116
CviJI RGCY 7 cut(s) 30, 35, 68, 148, 204, 210, 235
CviKI_1 RGCY 7 cut(s) 30, 35, 68, 148, 204, 210, 235
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 2 cut(s) 31, 36
DinI GGCGCC 1 cut(s) 103
DpnI GATC 3 cut(s) 41, 111, 121
DpnII GATC 3 cut(s) 39, 109, 119
Eco130I CCWWGG 2 cut(s) 115, 126
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 2 cut(s) 115, 126
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 2 cut(s) 115, 126
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 2 cut(s) 28, 119
FaiI YATR 2 cut(s) 26, 117
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FatI CATG 2 cut(s) 24, 115
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 2 cut(s) 69, 105
Fsp4HI GCNGC 2 cut(s) 69, 105
GlaI GCGC 1 cut(s) 103
GluI GCNGC 2 cut(s) 69, 105
GsaI CCCAGC 1 cut(s) 208
GsuI CTGGAG 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 105
HapII CCGG 1 cut(s) 99
HgaI GACGC 2 cut(s) 74, 268
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 3 cut(s) 5, 102, 260
Hin1II CATG 2 cut(s) 28, 119
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HpaII CCGG 1 cut(s) 99
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 2 cut(s) 133, 275
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 2 cut(s) 63, 139
Hpy8I GTNNAC 2 cut(s) 133, 275
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 99, 104, 184
HpyF3I CTNAG 2 cut(s) 31, 36
Hsp92I GRCGYC 3 cut(s) 5, 102, 260
Hsp92II CATG 2 cut(s) 28, 119
HspAI GCGC 1 cut(s) 102
KasI GGCGCC 1 cut(s) 101
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 3 cut(s) 39, 109, 119
LmnI GCTCC 2 cut(s) 65, 175
MalI GATC 3 cut(s) 41, 111, 121
MboI GATC 3 cut(s) 39, 109, 119
MflI RGATCY 2 cut(s) 39, 119
Mly113I GGCGCC 1 cut(s) 102
MnlI CCTC 3 cut(s) 70, 104, 166
MreI CGCCGGCG 1 cut(s) 98
MroNI GCCGGC 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 99, 104, 184
NaeI GCCGGC 1 cut(s) 100
NarI GGCGCC 1 cut(s) 102
NcoI CCATGG 1 cut(s) 115
NdeII GATC 3 cut(s) 39, 109, 119
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 2 cut(s) 28, 119
NlaIV GGNNCC 2 cut(s) 67, 103
PdiI GCCGGC 1 cut(s) 100
PkrI GCNGC 2 cut(s) 70, 106
PluTI GGCGCC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 204
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 2 cut(s) 67, 103
PsuI RGATCY 2 cut(s) 39, 119
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
SatI GCNGC 2 cut(s) 69, 105
Sau3AI GATC 3 cut(s) 39, 109, 119
ScrFI CCNGG 1 cut(s) 159
SetI ASST 4 cut(s) 32, 37, 132, 237
SfcI CTRYAG 1 cut(s) 220
SfoI GGCGCC 1 cut(s) 103
SgrAI CRCCGGYG 1 cut(s) 98
SsiI CCGC 2 cut(s) 69, 105
SspDI GGCGCC 1 cut(s) 101
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 2 cut(s) 115, 126
TaaI ACNGT 1 cut(s) 224
TatI WGTACW 1 cut(s) 272
TauI GCSGC 2 cut(s) 71, 107
TscAI CASTG 2 cut(s) 229, 247
TspRI CASTG 2 cut(s) 229, 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.