MD17G1082800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004468
Physical Location & Seq
Reverse (-)
6904089 .. 6906384
2296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1082800.v1.1.491

Sequence Viewer

Length: 381 bp
ATGACCAAAGCGTTTCCTCGTATTTCCCTTTCTCGCTCCTCTCTTCGTCTCCCTCTCTCTCATTTGAAGCTCAAAGGCATGGCTACTCTAGCGGCGGCCGCGGCTCGTCAACTCGCCACTTCAAGCCGCATCTCCACGGCGAGAACTTCGTCTCTCCCTTCCAATATAATCCACCGCCGCGGCCTCGCCGGCGCTGCTGATCACCATGGACCCCCAAGGGTTAACGTCTGGCAAGACCCATTGAGCCCATCTAAATGGAAGGAAGAGCATTTTGTAATTGTATCTTTATCTGGCTGGGGTCTACTCATCTTTGGAGGTTACAAGTTTTTCACTGGAGGCAAAGGCAAGAAAGAAGAGAACCTTGTGGAAGCAAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.74

Weight (kDa)

11.05

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 301
AccII CGCG 2 cut(s) 101, 180
AciI CCGC 8 cut(s) 92, 95, 99, 101, 127, 175, 178, 180
AcoI YGGCCR 1 cut(s) 96
AgsI TTSAA 2 cut(s) 67, 123
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
Alw26I GTCTC 2 cut(s) 53, 156
AoxI GGCC 2 cut(s) 96, 181
ApeKI GCWGC 1 cut(s) 194
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 194
AvaII GGWCC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 256
BceAI ACGGC 1 cut(s) 153
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 2 cut(s) 53, 156
BfaI CTAG 1 cut(s) 89
BfoI RGCGCY 1 cut(s) 195
BglI GCCNNNNNGGC 1 cut(s) 189
BisI GCNGC 8 cut(s) 93, 96, 99, 102, 127, 178, 181, 195
BlsI GCNGC 8 cut(s) 94, 97, 100, 103, 128, 179, 182, 196
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 211
BmsI GCATC 1 cut(s) 138
BpmI CTGGAG 1 cut(s) 354
BsaJI CCNNGG 5 cut(s) 99, 135, 178, 205, 215
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 337
BseDI CCNNGG 5 cut(s) 99, 135, 178, 205, 215
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 28
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 294
Bsh1236I CGCG 2 cut(s) 101, 180
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 98, 183
BsiEI CGRYCG 1 cut(s) 99
BsiSI CCGG 1 cut(s) 189
BsmAI GTCTC 2 cut(s) 53, 156
BsmBI CGTCTC 2 cut(s) 53, 156
BsnI GGCC 2 cut(s) 98, 183
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 1 cut(s) 199
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 8 cut(s) 92, 95, 99, 101, 127, 175, 178, 180
BspANI GGCC 2 cut(s) 98, 183
BspFNI CGCG 2 cut(s) 101, 180
BspLI GGNNCC 1 cut(s) 211
BspQI GCTCTTC 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 1 cut(s) 337
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 5 cut(s) 99, 135, 178, 205, 215
BssMI GATC 1 cut(s) 199
BssT1I CCWWGG 2 cut(s) 205, 215
Bst6I CTCTTC 3 cut(s) 48, 258, 348
BstC8I GCNNGC 1 cut(s) 190
BstDSI CCRYGG 4 cut(s) 99, 135, 178, 205
BstFNI CGCG 2 cut(s) 101, 180
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 1 cut(s) 202
BstMAI GTCTC 2 cut(s) 53, 156
BstMBI GATC 1 cut(s) 199
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 5 cut(s) 89, 98, 101, 189, 194
BstUI CGCG 2 cut(s) 101, 180
BstV1I GCAGC 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 255
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 2 cut(s) 98, 183
BtgI CCRYGG 4 cut(s) 99, 135, 178, 205
BtsIMutI CAGTG 2 cut(s) 330, 376
Cac8I GCNNGC 1 cut(s) 190
CciNI GCGGCCGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 209
Cfr42I CCGCGG 2 cut(s) 102, 181
CviAII CATG 2 cut(s) 79, 206
CviJI RGCY 8 cut(s) 70, 83, 98, 104, 126, 183, 246, 294
CviKI_1 RGCY 8 cut(s) 70, 83, 98, 104, 126, 183, 246, 294
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
EaeI YGGCCR 1 cut(s) 96
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 3 cut(s) 48, 258, 348
EarI CTCTTC 3 cut(s) 48, 258, 348
EclXI CGGCCG 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 205, 215
Eco24I GRGCYC 1 cut(s) 248
Eco47I GGWCC 1 cut(s) 209
Eco52I CGGCCG 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 205, 215
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 2 cut(s) 205, 215
Esp3I CGTCTC 2 cut(s) 53, 156
FaeI CATG 2 cut(s) 82, 209
FaiI YATR 3 cut(s) 80, 167, 207
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FatI CATG 2 cut(s) 78, 205
FbaI TGATCA 1 cut(s) 199
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 8 cut(s) 93, 96, 99, 102, 127, 178, 181, 195
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 8 cut(s) 93, 96, 99, 102, 127, 178, 181, 195
FspBI CTAG 1 cut(s) 89
GlaI GCGC 1 cut(s) 193
GluI GCNGC 8 cut(s) 93, 96, 99, 102, 127, 178, 181, 195
GsaI CCCAGC 1 cut(s) 298
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 2 cut(s) 98, 183
HapII CCGG 1 cut(s) 189
HhaI GCGC 1 cut(s) 194
Hin1II CATG 2 cut(s) 82, 209
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HincII GTYRAC 2 cut(s) 110, 223
HindII GTYRAC 2 cut(s) 110, 223
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 1 cut(s) 189
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 3 cut(s) 110, 223, 302
Hpy8I GTNNAC 3 cut(s) 110, 223, 302
HpyAV CCTTC 2 cut(s) 168, 253
HpyCH4IV ACGT 1 cut(s) 225
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 98, 101, 189, 194
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 2 cut(s) 82, 209
HspAI GCGC 1 cut(s) 192
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 223
KspI CCGCGG 2 cut(s) 102, 181
Kzo9I GATC 1 cut(s) 199
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 5 cut(s) 202, 214, 276, 280, 318
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 138
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 317
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 3 cut(s) 35, 275, 365
MhlI GDGCHC 1 cut(s) 248
MluCI AATT 1 cut(s) 276
MnlI CCTC 6 cut(s) 27, 49, 63, 194, 308, 329
MreI CGCCGGCG 1 cut(s) 188
MroNI GCCGGC 1 cut(s) 188
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 1 cut(s) 253
MspA1I CMGCKG 2 cut(s) 101, 180
MspI CCGG 1 cut(s) 189
MvnI CGCG 2 cut(s) 101, 180
MwoI GCNNNNNNNGC 5 cut(s) 89, 98, 101, 189, 194
NaeI GCCGGC 1 cut(s) 190
NcoI CCATGG 1 cut(s) 205
NdeII GATC 1 cut(s) 199
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 2 cut(s) 82, 209
NlaIV GGNNCC 1 cut(s) 211
NotI GCGGCCGC 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 258
PdiI GCCGGC 1 cut(s) 190
PkrI GCNGC 8 cut(s) 94, 97, 100, 103, 128, 179, 182, 196
PspFI CCCAGC 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 209
RseI CAYNNNNRTG 1 cut(s) 253
SacII CCGCGG 2 cut(s) 102, 181
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 8 cut(s) 93, 96, 99, 102, 127, 178, 181, 195
Sau3AI GATC 1 cut(s) 199
Sau96I GGNCC 1 cut(s) 209
SduI GDGCHC 1 cut(s) 248
SetI ASST 4 cut(s) 72, 228, 319, 363
SfaNI GCATC 1 cut(s) 138
Sfr303I CCGCGG 2 cut(s) 102, 181
SgrAI CRCCGGYG 1 cut(s) 188
SgrBI CCGCGG 2 cut(s) 102, 181
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 253
Sse9I AATT 1 cut(s) 276
SsiI CCGC 8 cut(s) 92, 95, 99, 101, 127, 175, 178, 180
SspMI CTAG 1 cut(s) 89
StyI CCWWGG 2 cut(s) 205, 215
TaiI ACGT 1 cut(s) 228
TasI AATT 1 cut(s) 276
TauI GCSGC 7 cut(s) 95, 98, 101, 104, 129, 180, 183
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 337
TseI GCWGC 1 cut(s) 194
TspRI CASTG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 301
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.