AT2G21410

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003618
Physical Location & Seq
Forward (+)
9162516 .. 9168556
6041 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21410.1

Sequence Viewer

Length: 2466 bp
ATGGCGGAGAGTCACGGTGGTGGTGGTGGTTGCTGTCCGCCGATGGATCTGATGCGGTCAGAACCGATGCAACTCGTTCAAGTCATTGTTCCCATGGAATCTGCTCATCTTACCGTCTCTTATCTCGGCGATCTCGGTCTCGTCCAGTTCAAAGATCTTAATTCTGAGAAGAGCCCATTTCAAAGGACTTATGCTGCTCAGATAAAAAGATGTGGGGAAATGGCACGAAAAATACGTTTCTTTAAAGAGCAAATGTCAAAGGCTGGAGTTACTCCCAAAGAGACCCTAGATAGAGAAAATGATATTGATTTGGATGATGTAGAGGTAAAGCTTGAAGAGCTTGAAGCTGAACTTGTTGAAATCAATGCTAATAACGACAAGTTGCAGCGGTCTTATAATGAACTTGTGGAGTACAAGCTCGTTCTTGAGAAGGCTGGTGAGTTTTTTGCTTCAGCCCATAGAAGTGCTACCGCTCAACAGAGTGAGATAGAAACAGAACAAGTGGGTGAAGACCTCCTGGAGGCTCCTCTGTTGCAGGAAGAGAAGTCTGTTGATCCCACAAAGCAAGTAAAGCTTGGATTCCTCACTGGACTAGTGCCTCGTGAAAAGTCTATGGTGTTCGAGAGGATCCTATTTCGTGCAACTAGGGGCAACATCTTCATACGACAGTCTGTCATTGAGGAGTCTGTTGTTGATCCCAACTCTGGGGAGAAGGCTGAGAAAAATGTATTTGTCGTCTTCTATTCTGGGGAAAGAGCAAAAAGCAAAATTCTTAAGATATGTGAAGCTTTTGGGGCCAATCGCTATCCTTTCAGTGAAGATCTGGGCAAACAAGCTCAAATGATGACTGAGGTTTCAGGTCGGTTATCAGAGCTTAAAACTACCATAGGTGCTGGGTTGGATCAGCGCAACATTCTTTTAGAGACCATTGGAGATAAGTTTGAGCAATGGAACCTCAAGATTCGCAAAGAAAAAGCTATCTATCACACCTTGAACATGCTTAGTCTTGATGTGACTAAGAAGTGCCTTGTGGGTGAAGGCTGGAGTCCTGTCTTTGCAGCAACAGAAATTCAAGATGCATTGCATCGTGCTGCGGTTGACTCCAATTCTCAAGTTGGATCAATTTTCCAAGTCCTGAGGACCAAAGAGATGCCTCCGACTTTTTTCCGCACAAACAAATTTACTACTGCTTTCCAGGAAATTGTTGATGCATACGGTGTGGCCAAATATCAGGAGGCCAATCCTAGTGTATTCACAATTGTTACCTTCCCCTTCCTGTTTGCTGTTATGTTTGGTGACTGGGGTCATGGAATCTGCCTGCTGCTTGCAACTATGTATTTAATATTGAGAGAAAAGAAACTTTCCAGCCAGAAACTTGGGGATATTATGGAAATGGCTTTTGGTGGCCGTTACGTTATTTTTATGATGTCACTATTCTCAATATACACTGGTTTAATCTACAACGAGTTCTTCTCTATACCATATCCATTGTTTGCTAGCTCAGCATACGATTGCCGTGATGTCTCTTGCAGTGAGGCTACTACAATTGGTCTGATCAAAACCCGAGACACTTATCCATTTGGTGTGGATCCTGTGTGGCATGGTACCCGCAGTGAGCTACCATTCCTAAATTCGCTTAAGATGAAAATGTCAATTCTTATTGGAGTTGCACAAATGAACCTCGGAATCATAATGAGCTTCTTTAATGCAAAATTCTTCAAAAGTGCTGTAAACATATGGTTCCAGTTCGTTCCCCAGATGATATTCTTGAACTGCTTGTTTGGCTATCTTTCGGTCCTCATCATCATAAAGTGGTGCACTGGTTCTCAAGCAGATTTATATCACGTAATGATCTACATGTTTCTGAGCCCTATGGATGATTTAGGAGAGAATCAGCTGTTCCCTAACCAGAAAATAGTACAGCTCACATTCCTCTTTCTGGCTCTGGTTTCTGTGCCGTGGATGTTGTTGCCAAAGCCATTCATCCTGAAAAAACAACATGAAGCTAGACATCAAGGACTGTCATATGCACAGCTTGACGAGACAGATGAGAGTCTTCAAGTAGAAACAAATGGGGGAGGACATGGACACGAAGAGTTTGAATTCAGCGAAATCTTTGTGCACCAACTCATTCACACCATTGAGTTTGTGCTTGGAGCTGTTTCCAACACTGCTTCTTATCTTCGTCTCTGGGCTCTCAGTCTCGCCCACTCGGAGTTGTCGTCAGTCTTCTATGAGAAGGTCCTTCTTATGGCTTGGGGTTTCAACAATGTCTTCATCTGGATCGTTGGAATTCTCGTCTTCATATTTGCGACTGTGGGAGTGCTTCTTGTGATGGAGACATTGAGTGCATTCCTTCACGCGTTGCGTCTTCACTGGGTGGAGTACCAGAACAAGTTCTACGAAGGCGATGGTTACAAGTTTGCTCCTTTCACTTTCACTCTCGTCGGAAACGAAGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005794 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009678 GO:0009705 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030641 GO:0031090 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

821

Amino Acids

93.1

Weight (kDa)

5.38

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 41 - 813 0.0 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
Acc65I GGTACC 1 cut(s) 1604
AccB1I GGYRCC 1 cut(s) 1604
AccBSI CCGCTC 1 cut(s) 473
AccII CGCG 1 cut(s) 2363
AciI CCGC 8 cut(s) 5, 38, 55, 388, 471, 1094, 1168, 1609
AcoI YGGCCR 2 cut(s) 1221, 1405
AcsI RAATTY 7 cut(s) 768, 1068, 1178, 1630, 1712, 2102, 2292
AcuI CTGAAG 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 2380
AfaI GTAC 4 cut(s) 413, 1606, 1920, 2387
AfiI CCNNNNNNNGG 5 cut(s) 520, 704, 705, 1939, 2251
AflII CTTAAG 2 cut(s) 773, 1637
AflIII ACRYGT 2 cut(s) 1857, 2361
AhdI GACNNNNNGTC 1 cut(s) 671
AhlI ACTAGT 1 cut(s) 592
AjnI CCWGG 2 cut(s) 516, 1194
AjuI GAANNNNNNNTTGG 2 cut(s) 1383, 1415
AleI CACNNNNGTG 1 cut(s) 18
Alw21I GWGCWC 2 cut(s) 1820, 2124
Alw44I GTGCAC 2 cut(s) 1816, 2120
Ama87I CYCGRG 1 cut(s) 1563
AoxI GGCC 4 cut(s) 795, 1221, 1236, 1405
ApaLI GTGCAC 2 cut(s) 1816, 2120
ApeKI GCWGC 5 cut(s) 194, 385, 1058, 1091, 1321
ApoI RAATTY 7 cut(s) 768, 1068, 1178, 1630, 1712, 2102, 2292
Asp700I GAANNNNTTC 1 cut(s) 2396
Asp718I GGTACC 1 cut(s) 1604
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 4 cut(s) 795, 1140, 1795, 2242
AsuHPI GGTGA 4 cut(s) 449, 518, 1046, 1307
AsuNHI GCTAGC 1 cut(s) 1496
AvaI CYCGRG 1 cut(s) 1563
AvaII GGWCC 3 cut(s) 1140, 1795, 2242
AxyI CCTNAGG 1 cut(s) 1136
BaeGI GKGCMC 2 cut(s) 1820, 2124
BalI TGGCCA 1 cut(s) 1223
BamHI GGATCC 2 cut(s) 627, 1588
BanI GGYRCC 1 cut(s) 1604
BanII GRGCYC 3 cut(s) 176, 1871, 2197
BauI CACGAG 1 cut(s) 600
BbsI GAAGAC 7 cut(s) 516, 730, 2048, 2221, 2266, 2293, 2363
Bbv12I GWGCWC 2 cut(s) 1820, 2124
BbvI GCAGC 5 cut(s) 181, 397, 1070, 1078, 1308
BccI CCATC 3 cut(s) 37, 2329, 2405
BceAI ACGGC 3 cut(s) 1392, 1500, 1942
BciT130I CCWGG 2 cut(s) 518, 1196
BclI TGATCA 1 cut(s) 1554
BcuI ACTAGT 1 cut(s) 592
BfaI CTAG 6 cut(s) 287, 593, 645, 1245, 1497, 2007
BfrI CTTAAG 2 cut(s) 773, 1637
BglII AGATCT 2 cut(s) 154, 820
BisI GCNGC 5 cut(s) 195, 386, 1059, 1092, 1322
BlpI GCTNAGC 1 cut(s) 1501
BlsI GCNGC 5 cut(s) 196, 387, 1060, 1093, 1323
Bme1390I CCNGG 2 cut(s) 518, 1196
Bme18I GGWCC 3 cut(s) 1140, 1795, 2242
BmeRI GACNNNNNGTC 1 cut(s) 671
BmeT110I CYCGRG 1 cut(s) 1563
BmgT120I GGNCC 4 cut(s) 795, 1140, 1795, 2242
BmiI GGNNCC 7 cut(s) 525, 629, 796, 953, 1590, 1606, 1742
BmrFI CCNGG 2 cut(s) 518, 1196
BmrI ACTGGG 2 cut(s) 1309, 2386
BmsI GCATC 6 cut(s) 42, 57, 1066, 1093, 1140, 1198
BmtI GCTAGC 1 cut(s) 1500
BmuI ACTGGG 2 cut(s) 1309, 2386
BoxI GACNNNNGTC 1 cut(s) 1302
BpiI GAAGAC 7 cut(s) 516, 730, 2048, 2221, 2266, 2293, 2363
BplI GAGNNNNNCTC 2 cut(s) 1457, 1489
BpmI CTGGAG 3 cut(s) 285, 539, 1063
Bpu1102I GCTNAGC 1 cut(s) 1501
BpuEI CTTGAG 4 cut(s) 446, 941, 1095, 1812
BsaAI YACGTR 1 cut(s) 1846
BsaBI GATNNNNATC 1 cut(s) 1839
BsaI GGTCTC 3 cut(s) 143, 275, 917
BsaJI CCNNGG 3 cut(s) 93, 1681, 1958
BsaXI ACNNNNNCTCC 2 cut(s) 2207, 2237
Bsc4I CCNNNNNNNGG 5 cut(s) 520, 704, 705, 1939, 2251
Bse1I ACTGG 7 cut(s) 145, 592, 1304, 1453, 1744, 1825, 2381
Bse21I CCTNAGG 1 cut(s) 1136
Bse3DI GCAATG 2 cut(s) 953, 1079
Bse8I GATNNNNATC 1 cut(s) 1839
BseBI CCWGG 2 cut(s) 518, 1196
BseDI CCNNGG 3 cut(s) 93, 1681, 1958
BseGI GGATG 4 cut(s) 319, 1882, 1968, 1983
BseJI GATNNNNATC 1 cut(s) 1839
BseLI CCNNNNNNNGG 5 cut(s) 520, 704, 705, 1939, 2251
BseMI GCAATG 2 cut(s) 953, 1079
BseMII CTCAG 8 cut(s) 156, 212, 708, 840, 1127, 1515, 1856, 2212
BseNI ACTGG 7 cut(s) 145, 592, 1304, 1453, 1744, 1825, 2381
BseRI GAGGAG 2 cut(s) 516, 695
BseSI GKGCMC 2 cut(s) 1820, 2124
BseXI GCAGC 5 cut(s) 181, 397, 1070, 1078, 1308
BseYI CCCAGC 1 cut(s) 893
Bsh1236I CGCG 1 cut(s) 2363
BshFI GGCC 4 cut(s) 797, 1223, 1238, 1407
BshNI GGYRCC 1 cut(s) 1604
BsiHKAI GWGCWC 2 cut(s) 1820, 2124
BsiHKCI CYCGRG 1 cut(s) 1563
BslI CCNNNNNNNGG 5 cut(s) 520, 704, 705, 1939, 2251
BsmBI CGTCTC 2 cut(s) 121, 2192
BsmI GAATGC 1 cut(s) 2351
BsnI GGCC 4 cut(s) 797, 1223, 1238, 1407
Bso31I GGTCTC 3 cut(s) 143, 275, 917
BsoBI CYCGRG 1 cut(s) 1563
Bsp1286I GDGCHC 5 cut(s) 176, 1820, 1871, 2124, 2197
Bsp1720I GCTNAGC 1 cut(s) 1501
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 8 cut(s) 5, 38, 55, 388, 471, 1094, 1168, 1609
BspANI GGCC 4 cut(s) 797, 1223, 1238, 1407
BspCNI CTCAG 8 cut(s) 157, 211, 709, 841, 1128, 1514, 1857, 2211
BspFNI CGCG 1 cut(s) 2363
BspLI GGNNCC 7 cut(s) 525, 629, 796, 953, 1590, 1606, 1742
BspOI GCTAGC 1 cut(s) 1500
BspQI GCTCTTC 2 cut(s) 164, 330
BspT107I GGYRCC 1 cut(s) 1604
BspTI CTTAAG 2 cut(s) 773, 1637
BspTNI GGTCTC 3 cut(s) 143, 275, 917
BsrBI CCGCTC 1 cut(s) 473
BsrDI GCAATG 2 cut(s) 953, 1079
BsrI ACTGG 7 cut(s) 145, 592, 1304, 1453, 1744, 1825, 2381
BssECI CCNNGG 3 cut(s) 93, 1681, 1958
BssSI CACGAG 1 cut(s) 600
BssT1I CCWWGG 1 cut(s) 93
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 518, 1196
Bst4CI ACNGT 6 cut(s) 17, 115, 669, 1217, 2022, 2317
Bst6I CTCTTC 4 cut(s) 164, 330, 534, 2088
BstAFI CTTAAG 2 cut(s) 773, 1637
BstBAI YACGTR 1 cut(s) 1846
BstC8I GCNNGC 3 cut(s) 1319, 1326, 1498
BstDSI CCRYGG 2 cut(s) 93, 1958
BstENI CCTNNNNNAGG 1 cut(s) 518
BstF5I GGATG 4 cut(s) 319, 1882, 1968, 1983
BstFNI CGCG 1 cut(s) 2363
BstHHI GCGC 1 cut(s) 909
BstMWI GCNNNNNNNGC 5 cut(s) 337, 571, 794, 1502, 1782
BstNI CCWGG 2 cut(s) 518, 1196
BstNSI RCATGY 2 cut(s) 1000, 1861
BstPAI GACNNNNGTC 1 cut(s) 1302
BstSCI CCNGG 2 cut(s) 516, 1194
BstSLI GKGCMC 2 cut(s) 1820, 2124
BstUI CGCG 1 cut(s) 2363
BstV1I GCAGC 5 cut(s) 181, 397, 1070, 1078, 1308
BstV2I GAAGAC 7 cut(s) 516, 730, 2048, 2221, 2266, 2293, 2363
BstX2I RGATCY 5 cut(s) 46, 154, 627, 820, 1588
BstXI CCANNNNNNTGG 1 cut(s) 1376
BstYI RGATCY 5 cut(s) 46, 154, 627, 820, 1588
Bsu36I CCTNAGG 1 cut(s) 1136
BsuRI GGCC 4 cut(s) 797, 1223, 1238, 1407
BtgI CCRYGG 2 cut(s) 93, 1958
BtgZI GCGATG 1 cut(s) 2424
BtsCI GGATG 4 cut(s) 319, 1882, 1968, 1983
BtsI GCAGTG 3 cut(s) 1537, 1618, 2169
BtsIMutI CAGTG 8 cut(s) 585, 820, 1446, 1537, 1618, 1818, 2169, 2374
Cac8I GCNNGC 3 cut(s) 1319, 1326, 1498
CfoI GCGC 1 cut(s) 909
Cfr13I GGNCC 4 cut(s) 795, 1140, 1795, 2242
CseI GACGC 1 cut(s) 2357
Csp6I GTAC 4 cut(s) 412, 1605, 1919, 2386
CviAII CATG 7 cut(s) 94, 997, 1307, 1601, 1858, 2000, 2084
CviQI GTAC 4 cut(s) 412, 1605, 1919, 2386
DraI TTTAAA 1 cut(s) 244
DraIII CACNNNGTG 1 cut(s) 2380
DriI GACNNNNNGTC 1 cut(s) 671
EaeI YGGCCR 2 cut(s) 1221, 1405
Eam1104I CTCTTC 4 cut(s) 164, 330, 534, 2088
Eam1105I GACNNNNNGTC 1 cut(s) 671
EarI CTCTTC 4 cut(s) 164, 330, 534, 2088
EciI GGCGGA 2 cut(s) 20, 27
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 3 cut(s) 176, 1871, 2197
Eco31I GGTCTC 3 cut(s) 143, 275, 917
Eco47I GGWCC 3 cut(s) 1140, 1795, 2242
Eco57I CTGAAG 1 cut(s) 435
Eco81I CCTNAGG 1 cut(s) 1136
Eco88I CYCGRG 1 cut(s) 1563
EcoNI CCTNNNNNAGG 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 2242
EcoRI GAATTC 2 cut(s) 2102, 2292
EcoRII CCWGG 2 cut(s) 516, 1194
EcoT14I CCWWGG 1 cut(s) 93
EcoT22I ATGCAT 2 cut(s) 1081, 1213
EcoT38I GRGCYC 3 cut(s) 176, 1871, 2197
ErhI CCWWGG 1 cut(s) 93
Esp3I CGTCTC 2 cut(s) 121, 2192
FaeI CATG 7 cut(s) 97, 1000, 1310, 1604, 1861, 2003, 2087
FalI AAGNNNNNCTT 4 cut(s) 336, 368, 558, 590
FatI CATG 7 cut(s) 93, 996, 1306, 1600, 1857, 1999, 2083
FauI CCCGC 1 cut(s) 1616
FauNDI CATATG 2 cut(s) 1736, 2026
FbaI TGATCA 1 cut(s) 1554
Fnu4HI GCNGC 5 cut(s) 195, 386, 1059, 1092, 1322
FokI GGATG 4 cut(s) 326, 1889, 1970, 1975
FriOI GRGCYC 3 cut(s) 176, 1871, 2197
Fsp4HI GCNGC 5 cut(s) 195, 386, 1059, 1092, 1322
FspBI CTAG 6 cut(s) 287, 593, 645, 1245, 1497, 2007
GlaI GCGC 1 cut(s) 908
GluI GCNGC 5 cut(s) 195, 386, 1059, 1092, 1322
GsaI CCCAGC 1 cut(s) 897
GsuI CTGGAG 3 cut(s) 285, 539, 1063
HaeIII GGCC 4 cut(s) 797, 1223, 1238, 1407
HgaI GACGC 1 cut(s) 2357
HhaI GCGC 1 cut(s) 909
Hin1II CATG 7 cut(s) 97, 1000, 1310, 1604, 1861, 2003, 2087
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HincII GTYRAC 1 cut(s) 1099
HindII GTYRAC 1 cut(s) 1099
HindIII AAGCTT 3 cut(s) 329, 572, 786
HphI GGTGA 4 cut(s) 449, 518, 1046, 1307
Hpy166II GTNNAC 4 cut(s) 1099, 1732, 1818, 2122
Hpy8I GTNNAC 4 cut(s) 1099, 1732, 1818, 2122
Hpy99I CGWCG 1 cut(s) 2450
HpyAV CCTTC 9 cut(s) 424, 706, 1031, 1276, 1282, 2233, 2255, 2366, 2399
HpyCH4III ACNGT 6 cut(s) 17, 115, 669, 1217, 2022, 2317
HpyCH4IV ACGT 3 cut(s) 235, 1413, 1845
HpyF10VI GCNNNNNNNGC 5 cut(s) 337, 571, 794, 1502, 1782
HpySE526I ACGT 3 cut(s) 235, 1413, 1845
Hsp92II CATG 7 cut(s) 97, 1000, 1310, 1604, 1861, 2003, 2087
HspAI GCGC 1 cut(s) 907
KpnI GGTACC 1 cut(s) 1608
Ksp22I TGATCA 1 cut(s) 1554
LguI GCTCTTC 2 cut(s) 164, 330
LmnI GCTCC 3 cut(s) 529, 2156, 2431
Lsp1109I GCAGC 5 cut(s) 181, 397, 1070, 1078, 1308
LweI GCATC 6 cut(s) 42, 57, 1066, 1093, 1140, 1198
MaeI CTAG 6 cut(s) 287, 593, 645, 1245, 1497, 2007
MaeII ACGT 3 cut(s) 235, 1413, 1845
MaeIII GTNAC 8 cut(s) 11, 268, 1012, 1261, 1295, 1409, 1428, 2414
MbiI CCGCTC 1 cut(s) 473
MfeI CAATTG 2 cut(s) 1257, 1545
MflI RGATCY 5 cut(s) 46, 154, 627, 820, 1588
MhlI GDGCHC 5 cut(s) 176, 1820, 1871, 2124, 2197
MlsI TGGCCA 1 cut(s) 1223
MluI ACGCGT 1 cut(s) 2361
MluNI TGGCCA 1 cut(s) 1223
MlyI GAGTC 5 cut(s) 19, 692, 1054, 1094, 2062
MmeI TCCRAC 6 cut(s) 879, 1096, 1181, 2190, 2269, 2428
Mox20I TGGCCA 1 cut(s) 1223
Mph1103I ATGCAT 2 cut(s) 1081, 1213
MroXI GAANNNNTTC 1 cut(s) 2396
MscI TGGCCA 1 cut(s) 1223
MseI TTAA 8 cut(s) 159, 243, 774, 876, 1340, 1454, 1638, 1704
MslI CAYNNNNRTG 2 cut(s) 18, 462
Msp20I TGGCCA 1 cut(s) 1223
MspA1I CMGCKG 2 cut(s) 388, 1897
MspCI CTTAAG 2 cut(s) 773, 1637
MspR9I CCNGG 2 cut(s) 518, 1196
MunI CAATTG 2 cut(s) 1257, 1545
Mva1269I GAATGC 1 cut(s) 2351
MvaI CCWGG 2 cut(s) 518, 1196
MvnI CGCG 1 cut(s) 2363
MwoI GCNNNNNNNGC 5 cut(s) 337, 571, 794, 1502, 1782
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 2 cut(s) 1736, 2026
NheI GCTAGC 1 cut(s) 1496
NlaIII CATG 7 cut(s) 97, 1000, 1310, 1604, 1861, 2003, 2087
NlaIV GGNNCC 7 cut(s) 525, 629, 796, 953, 1590, 1606, 1742
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 4 cut(s) 11, 1012, 1295, 1428
NsiI ATGCAT 2 cut(s) 1081, 1213
NspI RCATGY 2 cut(s) 1000, 1861
OliI CACNNNNGTG 1 cut(s) 18
PciI ACATGT 1 cut(s) 1857
PciSI GCTCTTC 2 cut(s) 164, 330
PcsI WCGNNNNNNNCGW 3 cut(s) 232, 2219, 2451
PctI GAATGC 1 cut(s) 2351
PdmI GAANNNNTTC 1 cut(s) 2396
PfeI GAWTC 6 cut(s) 98, 579, 961, 1311, 1686, 1891
PfoI TCCNGGA 2 cut(s) 516, 1194
PkrI GCNGC 5 cut(s) 196, 387, 1060, 1093, 1323
PleI GAGTC 5 cut(s) 18, 691, 1053, 1094, 2061
PpsI GAGTC 5 cut(s) 18, 691, 1053, 1094, 2061
Ppu21I YACGTR 1 cut(s) 1846
PpuMI RGGWCCY 1 cut(s) 2242
PscI ACATGT 1 cut(s) 1857
PshAI GACNNNNGTC 1 cut(s) 1302
PsiI TTATAA 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 2242
Psp6I CCWGG 2 cut(s) 516, 1194
PspFI CCCAGC 1 cut(s) 893
PspGI CCWGG 2 cut(s) 516, 1194
PspN4I GGNNCC 7 cut(s) 525, 629, 796, 953, 1590, 1606, 1742
PspPI GGNCC 4 cut(s) 795, 1140, 1795, 2242
PspPPI RGGWCCY 1 cut(s) 2242
PsrI GAACNNNNNNTAC 2 cut(s) 2384, 2416
PsuI RGATCY 5 cut(s) 46, 154, 627, 820, 1588
PvuII CAGCTG 1 cut(s) 1897
RsaI GTAC 4 cut(s) 413, 1606, 1920, 2387
RsaNI GTAC 4 cut(s) 412, 1605, 1919, 2386
RseI CAYNNNNRTG 2 cut(s) 18, 462
SapI GCTCTTC 2 cut(s) 164, 330
SaqAI TTAA 8 cut(s) 159, 243, 774, 876, 1340, 1454, 1638, 1704
SatI GCNGC 5 cut(s) 195, 386, 1059, 1092, 1322
Sau96I GGNCC 4 cut(s) 795, 1140, 1795, 2242
SchI GAGTC 5 cut(s) 19, 692, 1054, 1094, 2062
ScrFI CCNGG 2 cut(s) 518, 1196
SduI GDGCHC 5 cut(s) 176, 1820, 1871, 2124, 2197
SfaNI GCATC 6 cut(s) 42, 57, 1066, 1093, 1140, 1198
SinI GGWCC 3 cut(s) 1140, 1795, 2242
SmiMI CAYNNNNRTG 2 cut(s) 18, 462
SmlI CTYRAG 6 cut(s) 425, 773, 956, 1110, 1637, 1827
SmoI CTYRAG 6 cut(s) 425, 773, 956, 1110, 1637, 1827
SpeI ACTAGT 1 cut(s) 592
SsiI CCGC 8 cut(s) 5, 38, 55, 388, 471, 1094, 1168, 1609
SspI AATATT 1 cut(s) 1344
SspMI CTAG 6 cut(s) 287, 593, 645, 1245, 1497, 2007
StyD4I CCNGG 2 cut(s) 516, 1194
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 6 cut(s) 17, 115, 669, 1217, 2022, 2317
TaiI ACGT 3 cut(s) 238, 1416, 1848
TaqI TCGA 1 cut(s) 621
TaqII GACCGA 2 cut(s) 125, 1783
TatI WGTACW 2 cut(s) 411, 1918
TfiI GAWTC 6 cut(s) 98, 579, 961, 1311, 1686, 1891
Tru1I TTAA 8 cut(s) 159, 243, 774, 876, 1340, 1454, 1638, 1704
Tru9I TTAA 8 cut(s) 159, 243, 774, 876, 1340, 1454, 1638, 1704
TscAI CASTG 8 cut(s) 592, 820, 1453, 1537, 1618, 1825, 2176, 2381
TseFI GTSAC 4 cut(s) 11, 1012, 1295, 1428
TseI GCWGC 5 cut(s) 194, 385, 1058, 1091, 1321
Tsp45I GTSAC 4 cut(s) 11, 1012, 1295, 1428
TspDTI ATGAA 8 cut(s) 414, 649, 1658, 1691, 1972, 2016, 2266, 2293
TspRI CASTG 8 cut(s) 592, 820, 1453, 1537, 1618, 1825, 2176, 2381
Vha464I CTTAAG 2 cut(s) 773, 1637
VneI GTGCAC 2 cut(s) 1816, 2120
VpaK11BI GGWCC 3 cut(s) 1140, 1795, 2242
XagI CCTNNNNNAGG 1 cut(s) 518
XapI RAATTY 7 cut(s) 768, 1068, 1178, 1630, 1712, 2102, 2292
XceI RCATGY 2 cut(s) 1000, 1861
XmnI GAANNNNTTC 1 cut(s) 2396
XspI CTAG 6 cut(s) 287, 593, 645, 1245, 1497, 2007
Zsp2I ATGCAT 2 cut(s) 1081, 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.