Rmu_sc0013959.1_g000004

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013959.1
OG0003618
Physical Location & Seq
Forward (+)
15596 .. 25771
10176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013959.1_g000004.1.cds

Sequence Viewer

Length: 2421 bp
atggatctgttcaggtcggagcctatgcagctcgtccagatcatcatccccatcgagtccgcccacctcaccgtctcttatctcggcgacctcggcctcctccaattcaaagaccttaattcagagaagagcccgtttcagaggacatatgctactcagattaaaagagctggtgagatggcacgcaaattacgtttcttcaaggaccaaatgttaaaggctggtttgcctcctacaaagtctagaagacaagctgacctcaatgtagatgatctagaggtaaaacttggtgagctcgaggctgagctgattgaaattaatgcgaatggtgaaaaattgcagcgttcatataatgaactcttagaatataagcttgtcctgcaaaaggctggtgagttttttcattcagctgaaagcagcgccaggtcgcagcagagagatgaatcccgtcatactggtgatgacgccctggatacaccgttattggtggaccaagaatcatcaactgatccatcaaagcaagttaagttggggtttctcaccggtcttgttcctaggggaaagtctttggcatttgagaggattctatttcgagctactaggggtaatgtttttctaaggcaggctgttgttgagaatcctgttacagatccagtgacaggagagaagattgagaaaaatgtttttgttgtattctattctggagaaagagcaaagaacaagattcttaaaatttgtgatgcctttggagccaatcgttacccttttactgaggacctacgtaaacaagctcaaacaattaatgaggttacaggtaaactttcagagctaaagactactatagatattggagtctcacaccgggaaagtttgttacagactattggcgaacattatgagcaatggaaccatttggcgaggaaggagaaagccatacaccacactcttaatatgcttagcctcgatgtaaccaaaaaatgtcttgtggctgaggggtggagtcctatttttgcatcgaagcagatccaggaggcattgcagcgggcagcttttgactccaattctcaagttggagctatcttccaggttctgcatacaaaggaagcaccaccaacatatttccgcacaaacaagttcacttcttcatttcaagagattgttgatgcatatggtgtggccaagtatcaagaagcaaatcctgctgtatatactatcattacatttccatttctttttgctgtcatgtttggtgattggggacatggactatgtttgcttcttgcgacattatatttgatagtcagggaaaggaaattttctaatgagaaacttggagatatcatggagatggcatttggtggccgttatgttattctattgatgtcaattttctcaatctacactggtttcatctataatgagttcttctctgtcccatttgaactgtttggtcgctcagcatatgcatgccgtgatctttcttgcagagatgccactactgctggcttgattaaggtgggtcccacatacccatttggtctggatcctgtgtggcatggatctcgcagtgagctgccattcttaaactcattgaagatgaaaatgtcaattcttattggggtggtccaaatgaaccttggaattgtgataagttatttcaatgcaaagttctttcaaaatggtctgaacgtttggttccagtttgtccctcagctgatattcttaaacagtctatttggctacttgtctgttctgattgttatgaagtggtggactggttcaaaagttgatctataccatgtaatgatatatatgttcctcagtcctactgatgagctaggtgaaaatcagcttttcagtggtcagaaaacagttcagttagtgctattaggtttggctctaatagctgtaccatggatgctgcttccaaagcctttcctcatgaagaagcaacatgaagctcgtcaccaaggccaatcatatgctctacttgagagcactgaggagagtttgcaagtgaattcaaaccatgatgcccatggtcatgaggagtttgaatttagtgaaatttttgtacatcaactcatacacacaatagagtttgtacttggagcggtctcaaatacagcttcctatcttcgactatgggccctaagtcttgctcattcagaattatccagtgtgttctatgacaaggttctcctaatggcttttgggttcaacaattgggttatacgtattgtgggcatcttggtcttcgtttgcgccactgtcggtgttttgctactgatggaaactctcagtgctttccttcatgctttgcgacttcactgggtggaattccaaaacaagttttacgagggagatggttacaagttctatccattctcatttgtattgcatgacgatgaagacgagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

806

Amino Acids

91.78

Weight (kDa)

6.18

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 2114
AciI CCGC 4 cut(s) 60, 1042, 1123, 2114
AclI AACGTT 1 cut(s) 1689
AclWI GGATC 7 cut(s) 12, 503, 644, 1018, 1538, 1551, 1567
AcoI YGGCCR 2 cut(s) 1176, 1360
AcsI RAATTY 6 cut(s) 732, 1313, 2020, 2057, 2067, 2339
AcyI GRCGYC 1 cut(s) 465
AdeI CACNNNGTG 1 cut(s) 2335
AfaI GTAC 3 cut(s) 1911, 2076, 2106
AfiI CCNNNNNNNGG 2 cut(s) 385, 659
AgeI ACCGGT 1 cut(s) 542
AjnI CCWGG 4 cut(s) 422, 468, 1026, 1083
Alw21I GWGCWC 2 cut(s) 297, 2000
Alw26I GTCTC 3 cut(s) 79, 859, 2122
AlwI GGATC 7 cut(s) 12, 503, 644, 1018, 1538, 1551, 1567
AlwNI CAGNNNCTG 1 cut(s) 1090
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 5 cut(s) 94, 1176, 1360, 1972, 2148
ApaI GGGCCC 1 cut(s) 2152
ApeKI GCWGC 8 cut(s) 28, 340, 417, 430, 1039, 1046, 1573, 1921
ApoI RAATTY 6 cut(s) 732, 1313, 2020, 2057, 2067, 2339
ArsI GACNNNNNNTTYG 2 cut(s) 1276, 1308
AseI ATTAAT 2 cut(s) 318, 801
AsiGI ACCGGT 1 cut(s) 542
AspA2I CCTAGG 1 cut(s) 554
AspLEI GCGC 2 cut(s) 422, 2267
AspS9I GGNCC 7 cut(s) 205, 490, 775, 1520, 1624, 2148, 2149
AsuC2I CCSGG 1 cut(s) 863
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 5 cut(s) 205, 490, 775, 1520, 1624
AvrII CCTAGG 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 2152
BalI TGGCCA 1 cut(s) 1178
BamHI GGATCC 1 cut(s) 1543
BanII GRGCYC 3 cut(s) 134, 297, 2152
BbsI GAAGAC 2 cut(s) 253, 2248
Bbv12I GWGCWC 2 cut(s) 297, 2000
BbvCI CCTCAGC 2 cut(s) 990, 1710
BbvI GCAGC 8 cut(s) 40, 352, 429, 442, 1051, 1058, 1560, 1908
BccI CCATC 6 cut(s) 59, 172, 520, 1342, 2284, 2360
BceAI ACGGC 2 cut(s) 1347, 1455
BcgI CGANNNNNNTGC 2 cut(s) 1544, 1578
BciT130I CCWGG 4 cut(s) 424, 470, 1028, 1085
BciVI GTATCC 1 cut(s) 466
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 3 cut(s) 79, 859, 2122
BfaI CTAG 5 cut(s) 243, 275, 555, 600, 1838
BfmI CTRYAG 1 cut(s) 840
BfoI RGCGCY 1 cut(s) 423
BfuI GTATCC 1 cut(s) 466
BisI GCNGC 8 cut(s) 29, 341, 418, 431, 1040, 1047, 1574, 1922
BlnI CCTAGG 1 cut(s) 554
BlpI GCTNAGC 3 cut(s) 303, 956, 1456
BlsI GCNGC 8 cut(s) 30, 342, 419, 432, 1041, 1048, 1575, 1923
Bme1390I CCNGG 5 cut(s) 424, 470, 863, 1028, 1085
Bme18I GGWCC 5 cut(s) 205, 490, 775, 1520, 1624
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 7 cut(s) 205, 490, 775, 1520, 1624, 2148, 2149
BmiI GGNNCC 8 cut(s) 21, 751, 908, 1521, 1522, 1545, 1697, 2150
BmrFI CCNGG 5 cut(s) 424, 470, 863, 1028, 1085
BmrI ACTGGG 1 cut(s) 2341
BmsI GCATC 7 cut(s) 730, 1022, 1153, 1480, 1908, 2023, 2256
BmuI ACTGGG 1 cut(s) 2341
BpiI GAAGAC 2 cut(s) 253, 2248
BplI GAGNNNNNCTC 2 cut(s) 1412, 1444
BpmI CTGGAG 1 cut(s) 723
Bpu10I CCTNAGC 2 cut(s) 990, 1710
Bpu1102I GCTNAGC 3 cut(s) 303, 956, 1456
BpuEI CTTGAG 2 cut(s) 1050, 2012
BpuMI CCSGG 1 cut(s) 863
BsaAI YACGTR 2 cut(s) 782, 2237
BsaBI GATNNNNATC 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 2122
BsaJI CCNNGG 7 cut(s) 91, 468, 554, 1636, 1913, 1969, 2038
BsaWI WCCGGW 1 cut(s) 542
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 659
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 7 cut(s) 460, 653, 1408, 1699, 1780, 2178, 2336
Bse3DI GCAATG 2 cut(s) 908, 1034
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 4 cut(s) 424, 470, 1028, 1085
BseDI CCNNGG 7 cut(s) 91, 468, 554, 1636, 1913, 1969, 2038
BseGI GGATG 2 cut(s) 45, 1923
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 2 cut(s) 385, 659
BseMI GCAATG 2 cut(s) 908, 1034
BseMII CTCAG 9 cut(s) 170, 294, 762, 981, 1470, 1724, 1834, 1992, 2314
BseNI ACTGG 7 cut(s) 460, 653, 1408, 1699, 1780, 2178, 2336
BseRI GAGGAG 3 cut(s) 89, 2018, 2063
BseSI GKGCMC 1 cut(s) 2152
BseXI GCAGC 8 cut(s) 40, 352, 429, 442, 1051, 1058, 1560, 1908
BshFI GGCC 5 cut(s) 96, 1178, 1362, 1974, 2150
BshTI ACCGGT 1 cut(s) 542
BsiHKAI GWGCWC 2 cut(s) 297, 2000
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 2 cut(s) 543, 862
BslFI GGGAC 4 cut(s) 1272, 1418, 1506, 1691
BslI CCNNNNNNNGG 2 cut(s) 385, 659
BsmAI GTCTC 3 cut(s) 79, 859, 2122
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 4 cut(s) 1272, 1418, 1506, 1691
BsnI GGCC 5 cut(s) 96, 1178, 1362, 1974, 2150
Bso31I GGTCTC 1 cut(s) 2122
BsoBI CYCGRG 1 cut(s) 296
Bsp120I GGGCCC 1 cut(s) 2148
Bsp1286I GDGCHC 4 cut(s) 134, 297, 2000, 2152
Bsp1407I TGTACA 1 cut(s) 2074
Bsp1720I GCTNAGC 3 cut(s) 303, 956, 1456
Bsp19I CCATGG 2 cut(s) 1913, 2038
BspACI CCGC 4 cut(s) 60, 1042, 1123, 2114
BspANI GGCC 5 cut(s) 96, 1178, 1362, 1974, 2150
BspCNI CTCAG 9 cut(s) 169, 295, 763, 982, 1469, 1723, 1833, 1993, 2313
BspHI TCATGA 2 cut(s) 1941, 2044
BspLI GGNNCC 8 cut(s) 21, 751, 908, 1521, 1522, 1545, 1697, 2150
BspPI GGATC 7 cut(s) 12, 503, 644, 1018, 1538, 1551, 1567
BspQI GCTCTTC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 2122
BsrBI CCGCTC 1 cut(s) 2114
BsrDI GCAATG 2 cut(s) 908, 1034
BsrFI RCCGGY 1 cut(s) 542
BsrGI TGTACA 1 cut(s) 2074
BsrI ACTGG 7 cut(s) 460, 653, 1408, 1699, 1780, 2178, 2336
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 7 cut(s) 91, 468, 554, 1636, 1913, 1969, 2038
BssNI GRCGYC 1 cut(s) 465
BssT1I CCWWGG 5 cut(s) 554, 1636, 1913, 1969, 2038
Bst2UI CCWGG 4 cut(s) 424, 470, 1028, 1085
Bst4CI ACNGT 6 cut(s) 73, 480, 1446, 1730, 1873, 2272
Bst6I CTCTTC 1 cut(s) 122
BstACI GRCGYC 1 cut(s) 465
BstAPI GCANNNNNTGC 1 cut(s) 1199
BstAUI TGTACA 1 cut(s) 2074
BstBAI YACGTR 2 cut(s) 782, 2237
BstC8I GCNNGC 5 cut(s) 184, 624, 1044, 1468, 1504
BstDSI CCRYGG 2 cut(s) 1913, 2038
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 2 cut(s) 45, 1923
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 2 cut(s) 422, 2267
BstMAI GTCTC 3 cut(s) 79, 859, 2122
BstMWI GCNNNNNNNGC 8 cut(s) 28, 93, 379, 749, 1199, 1499, 1904, 1930
BstNI CCWGG 4 cut(s) 424, 470, 1028, 1085
BstNSI RCATGY 1 cut(s) 1470
BstSCI CCNGG 5 cut(s) 422, 468, 861, 1026, 1083
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 1 cut(s) 2152
BstSNI TACGTA 2 cut(s) 782, 2237
BstV1I GCAGC 8 cut(s) 40, 352, 429, 442, 1051, 1058, 1560, 1908
BstV2I GAAGAC 2 cut(s) 253, 2248
BstX2I RGATCY 5 cut(s) 4, 649, 1023, 1543, 1559
BstYI RGATCY 5 cut(s) 4, 649, 1023, 1543, 1559
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 5 cut(s) 96, 1178, 1362, 1974, 2150
BtgI CCRYGG 2 cut(s) 1913, 2038
BtsCI GGATG 2 cut(s) 45, 1923
BtsI GCAGTG 1 cut(s) 1573
BtsIMutI CAGTG 9 cut(s) 660, 1401, 1573, 1864, 1998, 2185, 2268, 2308, 2329
Cac8I GCNNGC 5 cut(s) 184, 624, 1044, 1468, 1504
CaiI CAGNNNCTG 1 cut(s) 1090
CciI TCATGA 2 cut(s) 1941, 2044
CfoI GCGC 2 cut(s) 422, 2267
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 7 cut(s) 205, 490, 775, 1520, 1624, 2148, 2149
CseI GACGC 1 cut(s) 473
Csp6I GTAC 3 cut(s) 1910, 2075, 2105
CspAI ACCGGT 1 cut(s) 542
CviQI GTAC 3 cut(s) 1910, 2075, 2105
DraIII CACNNNGTG 1 cut(s) 2335
EaeI YGGCCR 2 cut(s) 1176, 1360
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 295
Eco105I TACGTA 2 cut(s) 782, 2237
Eco130I CCWWGG 5 cut(s) 554, 1636, 1913, 1969, 2038
Eco24I GRGCYC 3 cut(s) 134, 297, 2152
Eco31I GGTCTC 1 cut(s) 2122
Eco32I GATATC 1 cut(s) 1339
Eco47I GGWCC 5 cut(s) 205, 490, 775, 1520, 1624
Eco53kI GAGCTC 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoO109I RGGNCCY 3 cut(s) 775, 1520, 2149
EcoRI GAATTC 2 cut(s) 2020, 2339
EcoRII CCWGG 4 cut(s) 422, 468, 1026, 1083
EcoRV GATATC 1 cut(s) 1339
EcoT14I CCWWGG 5 cut(s) 554, 1636, 1913, 1969, 2038
EcoT22I ATGCAT 2 cut(s) 1168, 1468
EcoT38I GRGCYC 3 cut(s) 134, 297, 2152
ErhI CCWWGG 5 cut(s) 554, 1636, 1913, 1969, 2038
Esp3I CGTCTC 1 cut(s) 79
FaqI GGGAC 4 cut(s) 1272, 1418, 1506, 1691
FauI CCCGC 1 cut(s) 1035
FauNDI CATATG 4 cut(s) 148, 1168, 1462, 1981
Fnu4HI GCNGC 8 cut(s) 29, 341, 418, 431, 1040, 1047, 1574, 1922
FokI GGATG 2 cut(s) 32, 1930
FriOI GRGCYC 3 cut(s) 134, 297, 2152
Fsp4HI GCNGC 8 cut(s) 29, 341, 418, 431, 1040, 1047, 1574, 1922
FspBI CTAG 5 cut(s) 243, 275, 555, 600, 1838
GlaI GCGC 2 cut(s) 421, 2266
GluI GCNGC 8 cut(s) 29, 341, 418, 431, 1040, 1047, 1574, 1922
GsuI CTGGAG 1 cut(s) 723
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 5 cut(s) 96, 1178, 1362, 1974, 2150
HapII CCGG 2 cut(s) 543, 862
HgaI GACGC 1 cut(s) 473
HhaI GCGC 2 cut(s) 422, 2267
Hin1I GRCGYC 1 cut(s) 465
Hin6I GCGC 2 cut(s) 420, 2265
HinP1I GCGC 2 cut(s) 420, 2265
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 9 cut(s) 56, 443, 497, 583, 637, 724, 852, 1000, 1055
HpaII CCGG 2 cut(s) 543, 862
Hpy166II GTNNAC 5 cut(s) 490, 785, 818, 1137, 1773
Hpy188I TCNGA 9 cut(s) 19, 124, 141, 159, 826, 1686, 1755, 1866, 2170
Hpy188III TCNNGA 9 cut(s) 37, 243, 275, 702, 1151, 1187, 1541, 1942, 2045
Hpy8I GTNNAC 5 cut(s) 490, 785, 818, 1137, 1773
HpyAV CCTTC 2 cut(s) 916, 2321
HpyCH4III ACNGT 6 cut(s) 73, 480, 1446, 1730, 1873, 2272
HpyCH4IV ACGT 4 cut(s) 193, 781, 1689, 2236
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 93, 379, 749, 1199, 1499, 1904, 1930
HpySE526I ACGT 4 cut(s) 193, 781, 1689, 2236
Hsp92I GRCGYC 1 cut(s) 465
HspAI GCGC 2 cut(s) 420, 2265
KflI GGGWCCC 1 cut(s) 1520
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 4 cut(s) 19, 749, 1073, 2111
Lsp1109I GCAGC 8 cut(s) 40, 352, 429, 442, 1051, 1058, 1560, 1908
LweI GCATC 7 cut(s) 730, 1022, 1153, 1480, 1908, 2023, 2256
MaeI CTAG 5 cut(s) 243, 275, 555, 600, 1838
MaeII ACGT 4 cut(s) 193, 781, 1689, 2236
MaeIII GTNAC 8 cut(s) 643, 655, 758, 808, 873, 967, 1964, 2369
MbiI CCGCTC 1 cut(s) 2114
MfeI CAATTG 1 cut(s) 2224
MflI RGATCY 5 cut(s) 4, 649, 1023, 1543, 1559
MhlI GDGCHC 4 cut(s) 134, 297, 2000, 2152
MlsI TGGCCA 1 cut(s) 1178
MluNI TGGCCA 1 cut(s) 1178
MlyI GAGTC 4 cut(s) 65, 861, 1009, 1049
MmeI TCCRAC 1 cut(s) 1051
Mox20I TGGCCA 1 cut(s) 1178
Mph1103I ATGCAT 2 cut(s) 1168, 1468
MscI TGGCCA 1 cut(s) 1178
MslI CAYNNNNRTG 5 cut(s) 456, 1346, 1465, 2043, 2406
Msp20I TGGCCA 1 cut(s) 1178
MspA1I CMGCKG 3 cut(s) 410, 1042, 1714
MspI CCGG 2 cut(s) 543, 862
MspR9I CCNGG 5 cut(s) 424, 470, 863, 1028, 1085
MunI CAATTG 1 cut(s) 2224
MvaI CCWGG 4 cut(s) 424, 470, 1028, 1085
MwoI GCNNNNNNNGC 8 cut(s) 28, 93, 379, 749, 1199, 1499, 1904, 1930
NciI CCSGG 1 cut(s) 863
NcoI CCATGG 2 cut(s) 1913, 2038
NdeI CATATG 4 cut(s) 148, 1168, 1462, 1981
NlaIV GGNNCC 8 cut(s) 21, 751, 908, 1521, 1522, 1545, 1697, 2150
NmeAIII GCCGAG 2 cut(s) 63, 72
NmuCI GTSAC 2 cut(s) 655, 1964
NsiI ATGCAT 2 cut(s) 1168, 1468
NspI RCATGY 1 cut(s) 1470
PaeI GCATGC 1 cut(s) 1470
PaeR7I CTCGAG 1 cut(s) 296
PagI TCATGA 2 cut(s) 1941, 2044
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 2 cut(s) 190, 2412
PfeI GAWTC 5 cut(s) 443, 497, 583, 637, 724
PfoI TCCNGGA 1 cut(s) 1026
PinAI ACCGGT 1 cut(s) 542
PkrI GCNGC 8 cut(s) 30, 342, 419, 432, 1041, 1048, 1575, 1923
PleI GAGTC 4 cut(s) 64, 860, 1008, 1049
PpsI GAGTC 4 cut(s) 64, 860, 1008, 1049
Ppu21I YACGTR 2 cut(s) 782, 2237
PpuMI RGGWCCY 2 cut(s) 775, 1520
PshBI ATTAAT 2 cut(s) 318, 801
Psp124BI GAGCTC 1 cut(s) 297
Psp1406I AACGTT 1 cut(s) 1689
Psp5II RGGWCCY 2 cut(s) 775, 1520
Psp6I CCWGG 4 cut(s) 422, 468, 1026, 1083
PspGI CCWGG 4 cut(s) 422, 468, 1026, 1083
PspN4I GGNNCC 8 cut(s) 21, 751, 908, 1521, 1522, 1545, 1697, 2150
PspOMI GGGCCC 1 cut(s) 2148
PspPI GGNCC 7 cut(s) 205, 490, 775, 1520, 1624, 2148, 2149
PspPPI RGGWCCY 2 cut(s) 775, 1520
PspXI VCTCGAGB 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 1090
PsuI RGATCY 5 cut(s) 4, 649, 1023, 1543, 1559
PvuII CAGCTG 2 cut(s) 410, 1714
RsaI GTAC 3 cut(s) 1911, 2076, 2106
RsaNI GTAC 3 cut(s) 1910, 2075, 2105
RseI CAYNNNNRTG 5 cut(s) 456, 1346, 1465, 2043, 2406
SacI GAGCTC 1 cut(s) 297
SapI GCTCTTC 1 cut(s) 122
SatI GCNGC 8 cut(s) 29, 341, 418, 431, 1040, 1047, 1574, 1922
Sau96I GGNCC 7 cut(s) 205, 490, 775, 1520, 1624, 2148, 2149
SchI GAGTC 4 cut(s) 65, 861, 1009, 1049
ScrFI CCNGG 5 cut(s) 424, 470, 863, 1028, 1085
SduI GDGCHC 4 cut(s) 134, 297, 2000, 2152
SfaNI GCATC 7 cut(s) 730, 1022, 1153, 1480, 1908, 2023, 2256
SfcI CTRYAG 1 cut(s) 840
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 5 cut(s) 205, 490, 775, 1520, 1624
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 5 cut(s) 456, 1346, 1465, 2043, 2406
SmlI CTYRAG 3 cut(s) 296, 1065, 1991
SmoI CTYRAG 3 cut(s) 296, 1065, 1991
SnaBI TACGTA 2 cut(s) 782, 2237
SphI GCATGC 1 cut(s) 1470
SsiI CCGC 4 cut(s) 60, 1042, 1123, 2114
SspMI CTAG 5 cut(s) 243, 275, 555, 600, 1838
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 5 cut(s) 422, 468, 861, 1026, 1083
StyI CCWWGG 5 cut(s) 554, 1636, 1913, 1969, 2038
TaaI ACNGT 6 cut(s) 73, 480, 1446, 1730, 1873, 2272
TaiI ACGT 4 cut(s) 196, 784, 1692, 2239
TaqI TCGA 6 cut(s) 54, 297, 592, 963, 1016, 2140
TatI WGTACW 2 cut(s) 2074, 2104
TfiI GAWTC 5 cut(s) 443, 497, 583, 637, 724
TscAI CASTG 9 cut(s) 660, 1408, 1573, 1864, 2005, 2185, 2275, 2308, 2336
TseFI GTSAC 2 cut(s) 655, 1964
TseI GCWGC 8 cut(s) 28, 340, 417, 430, 1039, 1046, 1573, 1921
Tsp45I GTSAC 2 cut(s) 655, 1964
TspRI CASTG 9 cut(s) 660, 1408, 1573, 1864, 2005, 2185, 2275, 2308, 2336
VpaK11BI GGWCC 5 cut(s) 205, 490, 775, 1520, 1624
VspI ATTAAT 2 cut(s) 318, 801
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 6 cut(s) 732, 1313, 2020, 2057, 2067, 2339
XbaI TCTAGA 2 cut(s) 242, 274
XceI RCATGY 1 cut(s) 1470
XcmI CCANNNNNNNNNTGG 2 cut(s) 1634, 2036
XhoI CTCGAG 1 cut(s) 296
XmaJI CCTAGG 1 cut(s) 554
XspI CTAG 5 cut(s) 243, 275, 555, 600, 1838
Zsp2I ATGCAT 2 cut(s) 1168, 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.