MD08G1021400.v1.1

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003618
Physical Location & Seq
Reverse (-)
1539241 .. 1544923
5683 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1021400.v1.1.491

Sequence Viewer

Length: 2469 bp
ATGGTGACGACGGAAGCGGCGGGAGGAGGGTGCTGTCCGACGATGGATCTGCTGCGATCGGAGCCAATGCAGTTAGTGCAACTCATCATTCCGATCGAGTCGTCTCGCCACGCCATCTCCTACCTCGGCGATCTCGGCCTCTTTCAATTCAAAGACCTCAATGCGGAAAAGAGCCCGTTCCAGCGAACATACGCCACTCAGATCAAACGGTGTGGTGAAATGGCTCGGAGGTTACGGTTCTTCAATGAACAAATGAAGAAGGCCGGCTTGTCGCCCTCAACAAGGTCCATGATTAGCAATGACATCGATCTCGATAACTTGGAGGTTAAACTTGGTGAACTTGAAGCCGATCTGTTAGAAATTAATGCGAACAATGAACAATTACAACGCACTTACAGTGAATTGTTAGAATATAAACTTGTTCTGCAGAAGGCTGGCGAGTTTTTTAATTCAGCTCAAAGCACTGCTGCAGCTCTGCAGAGAGAACTTGAAGTGCAGCATAGTATGGAAAGATCCATCGACAGTCCATTATTACTTGAACAAGAAATGACAACAGACCTATCAAAGCATGTTAAGCTTGGATCTGTTAGTGGTCTTGTTCCAAGAGAAAAATCGATGGCTTTTGAAAGGATTCTGTTTCGTGCAACCAGGGGTAATGTGTTTCTGAAGCAGGCTGTGGTCAATGACCGTGTCGTTGATCCTGTGTCAGGAGAGAAGGTTGAGAAAAATGTGTTTATCATCTTCTATTCTGGAGAAAGAGCAAAGAACAAAATTCTGAAAATATGTGAAGCTTTTGGAGCAAACCGTTACCCATTTACAGATGACTTGGGAAAACAATTTCAGATGATTAACGAGGTGTCTGGAAAACTTTCAGAGCTGAAGACTACCATAGATGCTGGATTGTTGCACAGAAGCAGTTTATTACAGACAATTGGCCATCAATATGAGCAGTGGAACCTTCTGGTGAAGAAGGAAAAATCCATTTACCATACGTTAAACATGCTAAGCATTGATGTGAGTAGAATGTGTCTTGTTGCAGAAGGTTGGTGTCCTGTATATGCATCAAACCAGATTCAAAACGCATTGCAGCGGGCATCCTTAGATAGCAACTCTCAACTTGGCGCCATATTCCAGGTTTTGCATACAAAGGAACCGCCACCGACATATTTCCGCACAAACAAATTCACTTCTGCTTTTCAAGAAATTGTTGATGCATACGGGGTCGCCAAGTATCAGGAGGCTAATCCAGGCGTATACACCATTATCACATTCCCTTTCCTTTTTGCTGTAATGTTTGGAGATTGGGGACATGGTATTTGCTTGTTGCTGGCATCACTCTATTTCATAATCCGGGAGAGGAAGTTTTCTAGCCAGAAACTCGGGGACATCGTAGAAATGACATTTGGTGGCCGTTATATTATTATGATGATGGCGCTGTTCTCAATTTACACAGGACTGATATATAATGAATTCTTTTCGGTCCCATTTGAGTTATTTGGGAAATCTGCCTATGGGTGCCGTGACCCATCTTGTAGAGACGCTACTACAGTCGGTTTGACTAAGGTTCGTGACACTTATCCGTTTGGTGTGGATCCCAAATGGCATGGTTCTCGATCTGAGTTACCATTTCTTAACTCATTGAAGATGAAGATGTCAATCCTACTTGGAGTAGCCCAGATGAATCTTGGAATCGTTTTGAGTTACTTTAATGCAAAATTCTTTGCGGATAAACTGAATATTTGGTACCAATTTGTTCCTCAGATGATATTTTTAAACAGCCTGTTTGGCTACCTTTCTCTCCTAATTATTGTGAAATGGTGCACTGGTTCACAAGCCGATTTGTACCATGTAATGATATACATGTTCCTGAGTCCTACTGACGACCTCGGTGAAAATCAGCTTTTTTGGGGGCAAAGATTCCTTCAGATTTTGTTACTGCTATCGGCTCTTGTTGCTGTACCATGGATGCTGTTTCCAAAGCCTTATTTCTTGAAGAAACAGCATGAGGAGAGGCACCAAGGTCAATCCTACGCACTGCTTCACAGCGGCGAGGACCCTCTTGACGAGGAGCACCACCCAAGTTTACATGGTCATGAGGAATTTGAATTCACCGAAGTCTTTGTACACCAACTCATACATACCATAGAATTTGTGCTTGGAGCGGTGTCTAATACAGCTTCTTATCTACGTTTATGGGCACTCAGTTTGGCGCATTCGGAGTTGTCCAGTGTATTTTACGACAAGGTTCTACTTCTAGCCTGGGGGTACAACAATGTGATCATTCTGATCATTGGCATAATCGTCTTTATATGCGCGACAGTCGGAGTGCTGCTAGTGATGGAGACCTTGAGTGCATTCTTACATGCCTTGAGGCTTCACTGGGTGGAGTTCCAGAACAAGTTCTATGAGGGCTCTGGTTACAAATTCTATCCCTTCTCATTTGCATTGCTCAGCGAGGAGGACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

823

Amino Acids

93.66

Weight (kDa)

6.03

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 41 - 814 2e-302 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1743
AccB1I GGYRCC 4 cut(s) 1121, 1515, 1743, 2013
AccBSI CCGCTC 1 cut(s) 2162
AccI GTMKAC 1 cut(s) 1254
AccII CGCG 1 cut(s) 2315
AciI CCGC 9 cut(s) 17, 20, 164, 1090, 1154, 1171, 1724, 2046, 2162
AclWI GGATC 6 cut(s) 54, 507, 589, 692, 1586, 1599
AcoI YGGCCR 2 cut(s) 934, 1408
AcsI RAATTY 8 cut(s) 771, 1181, 1469, 1715, 2099, 2105, 2147, 2423
AcuI CTGAAG 3 cut(s) 686, 899, 1907
AcyI GRCGYC 1 cut(s) 1122
AdeI CACNNNGTG 1 cut(s) 2383
AfaI GTAC 5 cut(s) 1745, 1844, 1959, 2124, 2267
AfiI CCNNNNNNNGG 3 cut(s) 163, 282, 707
AflIII ACRYGT 1 cut(s) 1860
AjnI CCWGG 4 cut(s) 647, 1131, 1246, 2258
AjuI GAANNNNNNNTTGG 4 cut(s) 1386, 1418, 2139, 2171
AluBI AGCT 7 cut(s) 455, 473, 577, 791, 877, 1900, 2177
AluI AGCT 7 cut(s) 455, 473, 577, 791, 877, 1900, 2177
Alw21I GWGCWC 2 cut(s) 1823, 2073
Alw26I GTCTC 3 cut(s) 108, 1530, 2336
Alw44I GTGCAC 1 cut(s) 1819
AlwI GGATC 6 cut(s) 54, 507, 589, 692, 1586, 1599
Ama87I CYCGRG 1 cut(s) 1379
AoxI GGCC 4 cut(s) 136, 261, 934, 1408
ApaLI GTGCAC 1 cut(s) 1819
ApeKI GCWGC 6 cut(s) 52, 467, 470, 496, 1087, 2329
ApoI RAATTY 8 cut(s) 771, 1181, 1469, 1715, 2099, 2105, 2147, 2423
ArsI GACNNNNNNTTYG 2 cut(s) 1299, 1331
AseI ATTAAT 1 cut(s) 363
Asp700I GAANNNNTTC 3 cut(s) 630, 868, 2399
Asp718I GGTACC 1 cut(s) 1743
AspLEI GCGC 4 cut(s) 1124, 1435, 2212, 2315
AspS9I GGNCC 3 cut(s) 285, 1480, 2053
AsuC2I CCSGG 1 cut(s) 1352
AsuHPI GGTGA 6 cut(s) 16, 227, 347, 976, 1901, 2101
AvaI CYCGRG 1 cut(s) 1379
AvaII GGWCC 3 cut(s) 285, 1480, 2053
BaeGI GKGCMC 2 cut(s) 1823, 2200
BalI TGGCCA 1 cut(s) 936
BamHI GGATCC 1 cut(s) 1591
BanI GGYRCC 4 cut(s) 1121, 1515, 1743, 2013
BanII GRGCYC 2 cut(s) 176, 2414
BarI GAAGNNNNNNTAC 2 cut(s) 2106, 2138
BbsI GAAGAC 1 cut(s) 887
Bbv12I GWGCWC 2 cut(s) 1823, 2073
BbvI GCAGC 6 cut(s) 39, 454, 482, 508, 1099, 2316
BccI CCATC 8 cut(s) 37, 122, 524, 610, 945, 1423, 1534, 2332
BceAI ACGGC 2 cut(s) 1395, 1503
BcgI CGANNNNNNTGC 6 cut(s) 31, 65, 286, 320, 1592, 1626
BciT130I CCWGG 4 cut(s) 649, 1133, 1248, 2260
BclI TGATCA 2 cut(s) 2277, 2286
BcnI CCSGG 1 cut(s) 1352
BcoDI GTCTC 3 cut(s) 108, 1530, 2336
BfaI CTAG 3 cut(s) 1368, 2255, 2333
BfmI CTRYAG 4 cut(s) 425, 468, 476, 1545
BfoI RGCGCY 2 cut(s) 1125, 1436
BglI GCCNNNNNGGC 1 cut(s) 1785
BisI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
BlpI GCTNAGC 2 cut(s) 1004, 2450
BlsI GCNGC 8 cut(s) 19, 54, 469, 472, 498, 1089, 2048, 2331
Bme1390I CCNGG 5 cut(s) 649, 1133, 1248, 1352, 2260
Bme18I GGWCC 3 cut(s) 285, 1480, 2053
BmeT110I CYCGRG 1 cut(s) 1379
BmgT120I GGNCC 3 cut(s) 285, 1480, 2053
BmrFI CCNGG 5 cut(s) 649, 1133, 1248, 1352, 2260
BmrI ACTGGG 1 cut(s) 2389
BmsI GCATC 6 cut(s) 883, 1070, 1103, 1201, 1340, 1956
BmuI ACTGGG 1 cut(s) 2389
BpiI GAAGAC 1 cut(s) 887
BpmI CTGGAG 1 cut(s) 771
Bpu1102I GCTNAGC 2 cut(s) 1004, 2450
BpuEI CTTGAG 2 cut(s) 2368, 2389
BpuMI CCSGG 1 cut(s) 1352
Bsa29I ATCGAT 2 cut(s) 306, 614
BsaBI GATNNNNATC 1 cut(s) 1655
BsaHI GRCGYC 1 cut(s) 1122
BsaI GGTCTC 1 cut(s) 2336
BsaJI CCNNGG 6 cut(s) 124, 648, 1885, 1961, 2017, 2259
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 702, 732, 1346, 1376
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 282, 707
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 1828, 2226, 2384
Bse3DI GCAATG 3 cut(s) 304, 1082, 2444
Bse8I GATNNNNATC 1 cut(s) 1655
BseBI CCWGG 4 cut(s) 649, 1133, 1248, 2260
BseCI ATCGAT 2 cut(s) 306, 614
BseDI CCNNGG 6 cut(s) 124, 648, 1885, 1961, 2017, 2259
BseGI GGATG 2 cut(s) 1094, 1971
BseJI GATNNNNATC 1 cut(s) 1655
BseLI CCNNNNNNNGG 3 cut(s) 163, 282, 707
BseMI GCAATG 3 cut(s) 304, 1082, 2444
BseMII CTCAG 6 cut(s) 212, 1608, 1772, 1859, 2215, 2464
BseNI ACTGG 3 cut(s) 1828, 2226, 2384
BseRI GAGGAG 3 cut(s) 39, 2021, 2081
BseSI GKGCMC 2 cut(s) 1823, 2200
BseXI GCAGC 6 cut(s) 39, 454, 482, 508, 1099, 2316
BsgI GTGCAG 1 cut(s) 515
Bsh1236I CGCG 1 cut(s) 2315
Bsh1285I CGRYCG 2 cut(s) 59, 96
BshFI GGCC 4 cut(s) 138, 263, 936, 1410
BshNI GGYRCC 4 cut(s) 1121, 1515, 1743, 2013
BshVI ATCGAT 2 cut(s) 306, 614
BsiEI CGRYCG 2 cut(s) 59, 96
BsiHKAI GWGCWC 2 cut(s) 1823, 2073
BsiHKCI CYCGRG 1 cut(s) 1379
BsiSI CCGG 2 cut(s) 264, 1351
BslFI GGGAC 3 cut(s) 1320, 1397, 1466
BslI CCNNNNNNNGG 3 cut(s) 163, 282, 707
BsmAI GTCTC 3 cut(s) 108, 1530, 2336
BsmBI CGTCTC 2 cut(s) 108, 1530
BsmFI GGGAC 3 cut(s) 1320, 1397, 1466
BsmI GAATGC 2 cut(s) 2212, 2354
BsnI GGCC 4 cut(s) 138, 263, 936, 1410
Bso31I GGTCTC 1 cut(s) 2336
BsoBI CYCGRG 1 cut(s) 1379
Bsp1286I GDGCHC 5 cut(s) 176, 1823, 2073, 2200, 2414
Bsp1407I TGTACA 1 cut(s) 2122
Bsp1720I GCTNAGC 2 cut(s) 1004, 2450
Bsp19I CCATGG 1 cut(s) 1961
BspACI CCGC 9 cut(s) 17, 20, 164, 1090, 1154, 1171, 1724, 2046, 2162
BspANI GGCC 4 cut(s) 138, 263, 936, 1410
BspCNI CTCAG 6 cut(s) 211, 1609, 1771, 1860, 2214, 2463
BspDI ATCGAT 2 cut(s) 306, 614
BspFNI CGCG 1 cut(s) 2315
BspHI TCATGA 1 cut(s) 2092
BspMAI CTGCAG 3 cut(s) 429, 472, 480
BspPI GGATC 6 cut(s) 54, 507, 589, 692, 1586, 1599
BspT107I GGYRCC 4 cut(s) 1121, 1515, 1743, 2013
BspTNI GGTCTC 1 cut(s) 2336
BsrBI CCGCTC 1 cut(s) 2162
BsrDI GCAATG 3 cut(s) 304, 1082, 2444
BsrFI RCCGGY 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 2122
BsrI ACTGG 3 cut(s) 1828, 2226, 2384
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 6 cut(s) 124, 648, 1885, 1961, 2017, 2259
BssNAI GTATAC 1 cut(s) 1255
BssNI GRCGYC 1 cut(s) 1122
BssT1I CCWWGG 2 cut(s) 1961, 2017
Bst1107I GTATAC 1 cut(s) 1255
Bst2UI CCWGG 4 cut(s) 649, 1133, 1248, 2260
Bst4CI ACNGT 8 cut(s) 210, 237, 398, 524, 689, 806, 1549, 2320
BstACI GRCGYC 1 cut(s) 1122
BstAPI GCANNNNNTGC 1 cut(s) 76
BstAUI TGTACA 1 cut(s) 2122
BstC8I GCNNGC 5 cut(s) 265, 436, 672, 1092, 1329
BstDEI CTNAG 9 cut(s) 198, 1004, 1099, 1560, 1617, 1758, 1868, 2201, 2450
BstDSI CCRYGG 1 cut(s) 1961
BstENI CCTNNNNNAGG 2 cut(s) 280, 705
BstF5I GGATG 2 cut(s) 1094, 1971
BstFNI CGCG 1 cut(s) 2315
BstH2I RGCGCY 2 cut(s) 1125, 1436
BstHHI GCGC 4 cut(s) 1124, 1435, 2212, 2315
BstMAI GTCTC 3 cut(s) 108, 1530, 2336
BstMCI CGRYCG 2 cut(s) 59, 96
BstMWI GCNNNNNNNGC 7 cut(s) 61, 76, 135, 574, 797, 1785, 1952
BstNI CCWGG 4 cut(s) 649, 1133, 1248, 2260
BstNSI RCATGY 4 cut(s) 572, 1003, 1864, 2366
BstSCI CCNGG 5 cut(s) 647, 1131, 1246, 1350, 2258
BstSFI CTRYAG 4 cut(s) 425, 468, 476, 1545
BstSLI GKGCMC 2 cut(s) 1823, 2200
BstUI CGCG 1 cut(s) 2315
BstV1I GCAGC 6 cut(s) 39, 454, 482, 508, 1099, 2316
BstV2I GAAGAC 1 cut(s) 887
BstX2I RGATCY 4 cut(s) 46, 512, 581, 1591
BstYI RGATCY 4 cut(s) 46, 512, 581, 1591
BstZ17I GTATAC 1 cut(s) 1255
Bsu15I ATCGAT 2 cut(s) 306, 614
BsuRI GGCC 4 cut(s) 138, 263, 936, 1410
BsuTUI ATCGAT 2 cut(s) 306, 614
BtgI CCRYGG 1 cut(s) 1961
BtsCI GGATG 2 cut(s) 1094, 1971
BtsI GCAGTG 3 cut(s) 462, 956, 2033
BtsIMutI CAGTG 7 cut(s) 403, 462, 956, 1821, 2033, 2233, 2377
Cac8I GCNNGC 5 cut(s) 265, 436, 672, 1092, 1329
CciI TCATGA 1 cut(s) 2092
CfoI GCGC 4 cut(s) 1124, 1435, 2212, 2315
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 285, 1480, 2053
ClaI ATCGAT 2 cut(s) 306, 614
CseI GACGC 1 cut(s) 1547
Csp6I GTAC 5 cut(s) 1744, 1843, 1958, 2123, 2266
CspCI CAANNNNNGTGG 2 cut(s) 193, 228
CviQI GTAC 5 cut(s) 1744, 1843, 1958, 2123, 2266
DdeI CTNAG 9 cut(s) 198, 1004, 1099, 1560, 1617, 1758, 1868, 2201, 2450
DinI GGCGCC 1 cut(s) 1123
DraI TTTAAA 1 cut(s) 1773
DraIII CACNNNGTG 1 cut(s) 2383
EaeI YGGCCR 2 cut(s) 934, 1408
Eco130I CCWWGG 2 cut(s) 1961, 2017
Eco24I GRGCYC 2 cut(s) 176, 2414
Eco31I GGTCTC 1 cut(s) 2336
Eco47I GGWCC 3 cut(s) 285, 1480, 2053
Eco57I CTGAAG 3 cut(s) 686, 899, 1907
Eco88I CYCGRG 1 cut(s) 1379
EcoNI CCTNNNNNAGG 2 cut(s) 280, 705
EcoO109I RGGNCCY 1 cut(s) 2053
EcoRI GAATTC 2 cut(s) 1469, 2105
EcoRII CCWGG 4 cut(s) 647, 1131, 1246, 2258
EcoT14I CCWWGG 2 cut(s) 1961, 2017
EcoT22I ATGCAT 2 cut(s) 1063, 1216
EcoT38I GRGCYC 2 cut(s) 176, 2414
EgeI GGCGCC 1 cut(s) 1123
EheI GGCGCC 1 cut(s) 1123
ErhI CCWWGG 2 cut(s) 1961, 2017
Esp3I CGTCTC 2 cut(s) 108, 1530
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 3 cut(s) 1320, 1397, 1466
FauI CCCGC 2 cut(s) 13, 1083
FbaI TGATCA 2 cut(s) 2277, 2286
FblI GTMKAC 1 cut(s) 1254
Fnu4HI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
FokI GGATG 2 cut(s) 1081, 1978
FriOI GRGCYC 2 cut(s) 176, 2414
Fsp4HI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
FspBI CTAG 3 cut(s) 1368, 2255, 2333
GlaI GCGC 4 cut(s) 1123, 1434, 2211, 2314
GluI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
GsuI CTGGAG 1 cut(s) 771
HaeII RGCGCY 2 cut(s) 1125, 1436
HaeIII GGCC 4 cut(s) 138, 263, 936, 1410
HapII CCGG 2 cut(s) 264, 1351
HgaI GACGC 1 cut(s) 1547
HhaI GCGC 4 cut(s) 1124, 1435, 2212, 2315
Hin1I GRCGYC 1 cut(s) 1122
Hin6I GCGC 4 cut(s) 1122, 1433, 2210, 2313
HinP1I GCGC 4 cut(s) 1122, 1433, 2210, 2313
HindIII AAGCTT 2 cut(s) 575, 789
HinfI GANTC 7 cut(s) 98, 631, 1072, 1681, 1689, 1870, 1917
HpaII CCGG 2 cut(s) 264, 1351
HphI GGTGA 6 cut(s) 16, 227, 347, 976, 1901, 2101
Hpy166II GTNNAC 6 cut(s) 338, 1255, 1821, 1829, 2084, 2125
Hpy8I GTNNAC 6 cut(s) 338, 1255, 1821, 1829, 2084, 2125
Hpy99I CGWCG 2 cut(s) 13, 43
HpyAV CCTTC 8 cut(s) 253, 424, 709, 964, 968, 1034, 1931, 2443
HpyCH4III ACNGT 8 cut(s) 210, 237, 398, 524, 689, 806, 1549, 2320
HpyCH4IV ACGT 2 cut(s) 992, 2188
HpyF10VI GCNNNNNNNGC 7 cut(s) 61, 76, 135, 574, 797, 1785, 1952
HpyF3I CTNAG 9 cut(s) 198, 1004, 1099, 1560, 1617, 1758, 1868, 2201, 2450
HpySE526I ACGT 2 cut(s) 992, 2188
Hsp92I GRCGYC 1 cut(s) 1122
HspAI GCGC 4 cut(s) 1122, 1433, 2210, 2313
KasI GGCGCC 1 cut(s) 1121
KpnI GGTACC 1 cut(s) 1747
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Ksp22I TGATCA 2 cut(s) 2277, 2286
LmnI GCTCC 4 cut(s) 61, 797, 2068, 2159
Lsp1109I GCAGC 6 cut(s) 39, 454, 482, 508, 1099, 2316
LweI GCATC 6 cut(s) 883, 1070, 1103, 1201, 1340, 1956
MaeI CTAG 3 cut(s) 1368, 2255, 2333
MaeII ACGT 2 cut(s) 992, 2188
MaeIII GTNAC 9 cut(s) 4, 231, 806, 1520, 1568, 1620, 1700, 1932, 2417
MbiI CCGCTC 1 cut(s) 2162
MboII GAAGA 8 cut(s) 232, 268, 733, 892, 979, 1654, 1660, 2005
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 4 cut(s) 46, 512, 581, 1591
MhlI GDGCHC 5 cut(s) 176, 1823, 2073, 2200, 2414
MlsI TGGCCA 1 cut(s) 936
MluNI TGGCCA 1 cut(s) 936
Mly113I GGCGCC 1 cut(s) 1122
MlyI GAGTC 2 cut(s) 107, 1879
MmeI TCCRAC 2 cut(s) 62, 2302
Mox20I TGGCCA 1 cut(s) 936
Mph1103I ATGCAT 2 cut(s) 1063, 1216
MroNI GCCGGC 1 cut(s) 263
MroXI GAANNNNTTC 3 cut(s) 630, 868, 2399
MscI TGGCCA 1 cut(s) 936
MseI TTAA 9 cut(s) 327, 363, 447, 573, 849, 995, 1632, 1707, 1772
MslI CAYNNNNRTG 3 cut(s) 942, 1013, 2091
Msp20I TGGCCA 1 cut(s) 936
MspA1I CMGCKG 2 cut(s) 1090, 2046
MspI CCGG 2 cut(s) 264, 1351
MspR9I CCNGG 5 cut(s) 649, 1133, 1248, 1352, 2260
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 2 cut(s) 2212, 2354
MvaI CCWGG 4 cut(s) 649, 1133, 1248, 2260
MvnI CGCG 1 cut(s) 2315
MwoI GCNNNNNNNGC 7 cut(s) 61, 76, 135, 574, 797, 1785, 1952
NaeI GCCGGC 1 cut(s) 265
NarI GGCGCC 1 cut(s) 1122
NciI CCSGG 1 cut(s) 1352
NcoI CCATGG 1 cut(s) 1961
NgoMIV GCCGGC 1 cut(s) 263
NmeAIII GCCGAG 2 cut(s) 105, 114
NmuCI GTSAC 3 cut(s) 4, 1520, 1568
NsiI ATGCAT 2 cut(s) 1063, 1216
NspI RCATGY 4 cut(s) 572, 1003, 1864, 2366
PagI TCATGA 1 cut(s) 2092
PciI ACATGT 1 cut(s) 1860
PcsI WCGNNNNNNNCGW 1 cut(s) 1386
PctI GAATGC 2 cut(s) 2212, 2354
PdiI GCCGGC 1 cut(s) 265
PdmI GAANNNNTTC 3 cut(s) 630, 868, 2399
PfeI GAWTC 5 cut(s) 631, 1072, 1681, 1689, 1917
PflFI GACNNNGTC 1 cut(s) 689
PfoI TCCNGGA 1 cut(s) 1350
PkrI GCNGC 8 cut(s) 19, 54, 469, 472, 498, 1089, 2048, 2331
Ple19I CGATCG 2 cut(s) 59, 96
PleI GAGTC 2 cut(s) 106, 1878
PluTI GGCGCC 1 cut(s) 1125
PpsI GAGTC 2 cut(s) 106, 1878
PpuMI RGGWCCY 1 cut(s) 2053
PscI ACATGT 1 cut(s) 1860
PshBI ATTAAT 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 2053
Psp6I CCWGG 4 cut(s) 647, 1131, 1246, 2258
PspGI CCWGG 4 cut(s) 647, 1131, 1246, 2258
PspPI GGNCC 3 cut(s) 285, 1480, 2053
PspPPI RGGWCCY 1 cut(s) 2053
PstI CTGCAG 3 cut(s) 429, 472, 480
PsuI RGATCY 4 cut(s) 46, 512, 581, 1591
PsyI GACNNNGTC 1 cut(s) 689
PvuI CGATCG 2 cut(s) 59, 96
RsaI GTAC 5 cut(s) 1745, 1844, 1959, 2124, 2267
RsaNI GTAC 5 cut(s) 1744, 1843, 1958, 2123, 2266
RseI CAYNNNNRTG 3 cut(s) 942, 1013, 2091
SaqAI TTAA 9 cut(s) 327, 363, 447, 573, 849, 995, 1632, 1707, 1772
SatI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
Sau96I GGNCC 3 cut(s) 285, 1480, 2053
SchI GAGTC 2 cut(s) 107, 1879
ScrFI CCNGG 5 cut(s) 649, 1133, 1248, 1352, 2260
SduI GDGCHC 5 cut(s) 176, 1823, 2073, 2200, 2414
SfaNI GCATC 6 cut(s) 883, 1070, 1103, 1201, 1340, 1956
SfcI CTRYAG 4 cut(s) 425, 468, 476, 1545
SfoI GGCGCC 1 cut(s) 1123
SinI GGWCC 3 cut(s) 285, 1480, 2053
SmiMI CAYNNNNRTG 3 cut(s) 942, 1013, 2091
SmlI CTYRAG 2 cut(s) 2347, 2368
SmoI CTYRAG 2 cut(s) 2347, 2368
SsiI CCGC 9 cut(s) 17, 20, 164, 1090, 1154, 1171, 1724, 2046, 2162
SspDI GGCGCC 1 cut(s) 1121
SspI AATATT 1 cut(s) 1738
SspMI CTAG 3 cut(s) 1368, 2255, 2333
StyD4I CCNGG 5 cut(s) 647, 1131, 1246, 1350, 2258
StyI CCWWGG 2 cut(s) 1961, 2017
TaaI ACNGT 8 cut(s) 210, 237, 398, 524, 689, 806, 1549, 2320
TaiI ACGT 2 cut(s) 995, 2191
TaqI TCGA 6 cut(s) 96, 306, 312, 519, 614, 1612
TaqII GACCGA 1 cut(s) 1468
TatI WGTACW 1 cut(s) 2122
TauI GCSGC 2 cut(s) 20, 2049
TfiI GAWTC 5 cut(s) 631, 1072, 1681, 1689, 1917
Tru1I TTAA 9 cut(s) 327, 363, 447, 573, 849, 995, 1632, 1707, 1772
Tru9I TTAA 9 cut(s) 327, 363, 447, 573, 849, 995, 1632, 1707, 1772
TscAI CASTG 7 cut(s) 403, 469, 956, 1828, 2040, 2233, 2384
TseFI GTSAC 3 cut(s) 4, 1520, 1568
TseI GCWGC 6 cut(s) 52, 467, 470, 496, 1087, 2329
Tsp45I GTSAC 3 cut(s) 4, 1520, 1568
TspDTI ATGAA 7 cut(s) 261, 269, 390, 1333, 1482, 1661, 1694
TspGWI ACGGA 2 cut(s) 26, 1569
TspRI CASTG 7 cut(s) 403, 469, 956, 1828, 2040, 2233, 2384
Tth111I GACNNNGTC 1 cut(s) 689
VneI GTGCAC 1 cut(s) 1819
VpaK11BI GGWCC 3 cut(s) 285, 1480, 2053
VspI ATTAAT 1 cut(s) 363
XagI CCTNNNNNAGG 2 cut(s) 280, 705
XapI RAATTY 8 cut(s) 771, 1181, 1469, 1715, 2099, 2105, 2147, 2423
XceI RCATGY 4 cut(s) 572, 1003, 1864, 2366
XcmI CCANNNNNNNNNTGG 1 cut(s) 1682
XmiI GTMKAC 1 cut(s) 1254
XmnI GAANNNNTTC 3 cut(s) 630, 868, 2399
XspI CTAG 3 cut(s) 1368, 2255, 2333
Zsp2I ATGCAT 2 cut(s) 1063, 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.