pycom05g04990

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003618
Physical Location & Seq
Forward (+)
5893823 .. 5903426
9604 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04990.18

Sequence Viewer

Length: 2445 bp
ATGGGAGAGTGCTGTCCTCCGATGGATCTGTTCAGGTCGGAGCCCATGCAGCTCGTCCAGATCATCATCCCCATTGAGTCCGCTCACCTCACCGTCTCTTATCTCGGCGACCTCGGCCTCCTCCAGTTCAAAGACCTCAATTCAGATAAGAGCCCATTTCAAAGGACATATGCTGCTCAGATTAAAAGAAGTGCTGAGATGGCACGCAAATTACGTTTCTTTAAGGAGCAAATGTCAAGGGCAGATTTACCTCCTTCAAAGTCTGCAAGACAAGTTGACGTCAATGTGGATGACCTTGAGGTGAAACTTGGTGAACTTGAGGCAGAGCTGATTGAAATAAATTCGAATGATGAAAAGTTGCAACGTTCATATAATGAACTCTTAGAATATAAGCTTGTTCTGGAAAAGGCTGGTGAGTTTTTTCACTCAGCTCAAAGCAGTGCCGTGTCGCAGCAGAGAGAAAATGAATCCCGTGATATCAGTGATGAATCCCTGGACACGCCTTTATTATTGGAACAAGAAACATCAACGGATCCATCAAAGCAAGTTAAGTTGGGATTTCTCACTGGTCTTGTTCCTAGGGAAAAATGTTTGGCATTTGAGAGGATTCTATTTCGAGCTACTAGGGGTAACGTTTTTCTGAGGCAGACTGTTGTTGAGAATCCCGTGACTGACCCTGTCTCAGGAGAGAAGGTTGAGAAAAATGTTTTTGTGGTATTCTACTCTGGAGAAAGAGCAAAGAGCAAAATCCTTAAAATATGTGATGCCTTTGGAGCTAATCGATACTCTTTCCCTGAGGACCTAGGTAGACAATCTCAGATGATTACTGAGGTTTCGGGAAGACTTTCAGAGCTAAAGACTACCATAGATGTTGGAGTGTTGCTTCGGGGACGTTTGCTACAGACTATTGGCGAGCATTTTGAGCAGTGGAACCTTTTGGTGAGGAAGGAGAAATCCATATACCACCATCTTAACATGCTCAGCCTTGATGTAACAAAAAAATGTCTTGTGGCTGAAGGGTGGAGTCCTGTTTTTGCATCAAAACAGATCCAGGATGCATTGCAGCGGGCAGCATTTGACTCTAACTCACAAGTTGGAGCTATTTTCCAGGTTTTGCATACTAAGGAGGCACCACCTACCTATTTCCGTACAAACAAATTCACTTCTTCTTTTCAAGAAATTGTTGATGCATACGGGGTGGCTAAGTATCAAGAAGCAAATCCTGCTGTATATACCATCGTGACATTCCCATTTCTTTTTGCTGTCATGTTTGGTGATTGGGGGCATGGAATATGTTTGTTACTTGCGACCTTATATTTAATAGGCAGGGAAAAGAAGCTTTCCAGTCAGAAGCTTGGAGACATCATGGAGATGGCCTTTGGTGGTCGTTACGTGATTTTGTTGATGGCAATTTTCTCAATCTACACAGGTGTTATCTATAATGAGTTCTTCTCTGTCCCGTTTGAACTGTTTGGTCCCTCAGCGTATGCATGCCGTGATCTTTCTTGCAGAGACGCTACTACAGCTGGCTTGGTTAAGGTGCGTCCTACTTATCCATTTGGCCTGGATCCTGTATGGCATGGATCCCGCAGTGAGCTCCCATTCCTAAACTCATTGAAGATGAAAATGTCAATTATTATTGGTGTGGTCCAGATGAACCTAGGAATTATGATAAGTTATTTCAATGCCAGGTTCTTTCGAAGTAATTTGAATGTTTGGTTCCAGTTTGTACCCCAGATGATATTTTTAAACAGTCTATTTGGCTACTTGTCCATTCTCATTGTTATGAAGTGGTGGACCGGTTCAAAAGCTGATCTCTACCACGTAATGATATACATGTTCCTAAGTCCTACAGACGAGCTAGGTGAAAATCAGCTTTTCAATGGTCAGAAAACAGTTCAGCTTGTGCTATTATTGTTGGCTTTTGTTGCTGTACCATGGATGCTGTTTCCAAAGCCTTTTCTTTTGAAGAAGCAACATCAAGATCGGCATCAAGGTCAGTCCTACGCTCTACTTGAGGATACAGAAGAGAGCTTGCAAGTGAATTCAAACCATGATGCCCATGGTCATGAGGAGTTTGAGTTCAGTGAAGTTTTCGTACATCAAATGATACACACAATAGAATTTGTACTTGGAGCAGTCTCAAATACAGCTTCATACCTTCGTCTATGGGCTTTAAGTCTTGCTCATTCAGAGTTGTCCAGTGTGTTCTACGACAAGGTTCTCCTAATGGCCTGGGGGTTCAACAATTGGATTATACTCATAGTGGGTCTCATCGTCTTTGTGTGCGCCACTGTCGGTGTTTTACTACTGATGGAAACTCTCAGTGCTTTCCTTCATGCTCTTCGACTTCACTGGGTGGAATTCCAGAACAAGTTTTATGAAGGGGATGGTTACAAGTTCTATCCATACTCGTTTGCATTGCTTGACGACGAAGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

815

Amino Acids

92.87

Weight (kDa)

5.72

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 1129
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 808
AciI CCGC 3 cut(s) 81, 1066, 1588
AclI AACGTT 2 cut(s) 364, 633
AclWI GGATC 8 cut(s) 33, 527, 540, 1042, 1562, 1575, 1578, 1591
AcsI RAATTY 5 cut(s) 340, 1157, 2044, 2123, 2363
AcuI CTGAAG 1 cut(s) 1035
AcyI GRCGYC 1 cut(s) 279
AdeI CACNNNGTG 1 cut(s) 2359
AfaI GTAC 5 cut(s) 1150, 1731, 1935, 2100, 2130
AfiI CCNNNNNNNGG 1 cut(s) 683
AflIII ACRYGT 1 cut(s) 1836
AgeI ACCGGT 1 cut(s) 1799
AjnI CCWGG 6 cut(s) 492, 1050, 1107, 1563, 1688, 2234
AjuI GAANNNNNNNTTGG 2 cut(s) 2115, 2147
AloI GAACNNNNNNTCC 2 cut(s) 2066, 2098
Alw21I GWGCWC 1 cut(s) 1599
Alw26I GTCTC 6 cut(s) 100, 685, 1353, 1506, 2146, 2276
AlwI GGATC 8 cut(s) 33, 527, 540, 1042, 1562, 1575, 1578, 1591
AoxI GGCC 4 cut(s) 115, 1374, 1561, 2232
ApeKI GCWGC 5 cut(s) 49, 173, 451, 1063, 1070
ApoI RAATTY 5 cut(s) 340, 1157, 2044, 2123, 2363
AsiGI ACCGGT 1 cut(s) 1799
Asp700I GAANNNNTTC 1 cut(s) 844
AspA2I CCTAGG 3 cut(s) 578, 802, 1660
AspLEI GCGC 1 cut(s) 2291
AspS9I GGNCC 4 cut(s) 799, 1475, 1648, 1797
AsuHPI GGTGA 8 cut(s) 77, 82, 313, 323, 425, 952, 1286, 1877
AsuII TTCGAA 2 cut(s) 344, 1699
AvaII GGWCC 4 cut(s) 799, 1475, 1648, 1797
AvrII CCTAGG 3 cut(s) 578, 802, 1660
AxyI CCTNAGG 1 cut(s) 795
BamHI GGATCC 3 cut(s) 532, 1567, 1583
BanI GGYRCC 1 cut(s) 1129
BanII GRGCYC 3 cut(s) 45, 155, 1599
BarI GAAGNNNNNNTAC 2 cut(s) 2082, 2114
BbsI GAAGAC 1 cut(s) 847
Bbv12I GWGCWC 1 cut(s) 1599
BbvCI CCTCAGC 1 cut(s) 1480
BbvI GCAGC 5 cut(s) 61, 160, 463, 1075, 1082
BccI CCATC 9 cut(s) 16, 193, 544, 975, 1244, 1366, 1399, 2308, 2384
BceAI ACGGC 2 cut(s) 428, 1479
BciT130I CCWGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
BciVI GTATCC 1 cut(s) 2014
BcoDI GTCTC 6 cut(s) 100, 685, 1353, 1506, 2146, 2276
BfaI CTAG 5 cut(s) 579, 624, 803, 1661, 1862
BfmI CTRYAG 3 cut(s) 899, 1521, 1851
BfuI GTATCC 1 cut(s) 2014
BisI GCNGC 5 cut(s) 50, 174, 452, 1064, 1071
BlnI CCTAGG 3 cut(s) 578, 802, 1660
BlpI GCTNAGC 1 cut(s) 980
BlsI GCNGC 5 cut(s) 51, 175, 453, 1065, 1072
Bme1390I CCNGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
Bme18I GGWCC 4 cut(s) 799, 1475, 1648, 1797
BmgT120I GGNCC 4 cut(s) 799, 1475, 1648, 1797
BmiI GGNNCC 8 cut(s) 42, 534, 932, 1131, 1477, 1569, 1585, 1721
BmrFI CCNGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
BmrI ACTGGG 1 cut(s) 2365
BmsI GCATC 7 cut(s) 754, 1045, 1046, 1177, 1932, 1999, 2047
BmuI ACTGGG 1 cut(s) 2365
BpiI GAAGAC 1 cut(s) 847
BplI GAGNNNNNCTC 2 cut(s) 1436, 1468
BpmI CTGGAG 2 cut(s) 107, 747
Bpu10I CCTNAGC 1 cut(s) 1480
Bpu1102I GCTNAGC 1 cut(s) 980
Bpu14I TTCGAA 2 cut(s) 344, 1699
BpuEI CTTGAG 3 cut(s) 317, 338, 2036
Bsa29I ATCGAT 1 cut(s) 781
BsaAI YACGTR 2 cut(s) 1393, 1825
BsaBI GATNNNNATC 2 cut(s) 65, 1989
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 2276
BsaJI CCNNGG 8 cut(s) 112, 492, 578, 802, 1660, 1937, 2062, 2235
BsaWI WCCGGW 1 cut(s) 1799
BsaXI ACNNNNNCTCC 6 cut(s) 218, 248, 678, 708, 2066, 2096
Bsc4I CCNNNNNNNGG 1 cut(s) 683
Bse118I RCCGGY 1 cut(s) 1799
Bse1I ACTGG 6 cut(s) 124, 571, 1344, 1723, 2202, 2360
Bse21I CCTNAGG 1 cut(s) 795
Bse3DI GCAATG 2 cut(s) 1058, 2420
Bse8I GATNNNNATC 2 cut(s) 65, 1989
BseBI CCWGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
BseCI ATCGAT 1 cut(s) 781
BseDI CCNNGG 8 cut(s) 112, 492, 578, 802, 1660, 1937, 2062, 2235
BseGI GGATG 5 cut(s) 66, 295, 1060, 1947, 2395
BseJI GATNNNNATC 2 cut(s) 65, 1989
BseLI CCNNNNNNNGG 1 cut(s) 683
BseMI GCAATG 2 cut(s) 1058, 2420
BseNI ACTGG 6 cut(s) 124, 571, 1344, 1723, 2202, 2360
BseRI GAGGAG 2 cut(s) 110, 2087
BseXI GCAGC 5 cut(s) 61, 160, 463, 1075, 1082
BshFI GGCC 4 cut(s) 117, 1376, 1563, 2234
BshNI GGYRCC 1 cut(s) 1129
BshTI ACCGGT 1 cut(s) 1799
BshVI ATCGAT 1 cut(s) 781
BsiHKAI GWGCWC 1 cut(s) 1599
BsiSI CCGG 1 cut(s) 1800
BslFI GGGAC 3 cut(s) 903, 1442, 1461
BslI CCNNNNNNNGG 1 cut(s) 683
BsmAI GTCTC 6 cut(s) 100, 685, 1353, 1506, 2146, 2276
BsmBI CGTCTC 2 cut(s) 100, 1506
BsmFI GGGAC 3 cut(s) 903, 1442, 1461
BsnI GGCC 4 cut(s) 117, 1376, 1563, 2234
Bso31I GGTCTC 1 cut(s) 2276
Bsp119I TTCGAA 2 cut(s) 344, 1699
Bsp1286I GDGCHC 3 cut(s) 45, 155, 1599
Bsp143I GATC 9 cut(s) 25, 60, 532, 1047, 1498, 1567, 1583, 1813, 1984
Bsp1720I GCTNAGC 1 cut(s) 980
Bsp19I CCATGG 2 cut(s) 1937, 2062
BspACI CCGC 3 cut(s) 81, 1066, 1588
BspANI GGCC 4 cut(s) 117, 1376, 1563, 2234
BspDI ATCGAT 1 cut(s) 781
BspHI TCATGA 1 cut(s) 2068
BspLI GGNNCC 8 cut(s) 42, 534, 932, 1131, 1477, 1569, 1585, 1721
BspPI GGATC 8 cut(s) 33, 527, 540, 1042, 1562, 1575, 1578, 1591
BspQI GCTCTTC 1 cut(s) 2349
BspT104I TTCGAA 2 cut(s) 344, 1699
BspT107I GGYRCC 1 cut(s) 1129
BspTNI GGTCTC 1 cut(s) 2276
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 1058, 2420
BsrFI RCCGGY 1 cut(s) 1799
BsrI ACTGG 6 cut(s) 124, 571, 1344, 1723, 2202, 2360
BssAI RCCGGY 1 cut(s) 1799
BssECI CCNNGG 8 cut(s) 112, 492, 578, 802, 1660, 1937, 2062, 2235
BssMI GATC 9 cut(s) 25, 60, 532, 1047, 1498, 1567, 1583, 1813, 1984
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 5 cut(s) 578, 802, 1660, 1937, 2062
Bst2UI CCWGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
Bst4CI ACNGT 6 cut(s) 94, 652, 1470, 1754, 1897, 2296
Bst6I CTCTTC 2 cut(s) 2022, 2349
BstACI GRCGYC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 1223
BstBAI YACGTR 2 cut(s) 1393, 1825
BstBI TTCGAA 2 cut(s) 344, 1699
BstC8I GCNNGC 6 cut(s) 205, 914, 1068, 1492, 1528, 2036
BstDSI CCRYGG 2 cut(s) 1937, 2062
BstENI CCTNNNNNAGG 1 cut(s) 681
BstF5I GGATG 5 cut(s) 66, 295, 1060, 1947, 2395
BstHHI GCGC 1 cut(s) 2291
BstKTI GATC 9 cut(s) 28, 63, 535, 1050, 1501, 1570, 1586, 1816, 1987
BstMAI GTCTC 6 cut(s) 100, 685, 1353, 1506, 2146, 2276
BstMBI GATC 9 cut(s) 25, 60, 532, 1047, 1498, 1567, 1583, 1813, 1984
BstMWI GCNNNNNNNGC 8 cut(s) 49, 114, 200, 773, 922, 1223, 1523, 1928
BstNI CCWGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
BstNSI RCATGY 3 cut(s) 979, 1494, 1840
BstSCI CCNGG 6 cut(s) 492, 1050, 1107, 1563, 1688, 2234
BstSFI CTRYAG 3 cut(s) 899, 1521, 1851
BstV1I GCAGC 5 cut(s) 61, 160, 463, 1075, 1082
BstV2I GAAGAC 1 cut(s) 847
BstX2I RGATCY 5 cut(s) 25, 532, 1047, 1567, 1583
BstYI RGATCY 5 cut(s) 25, 532, 1047, 1567, 1583
Bsu15I ATCGAT 1 cut(s) 781
Bsu36I CCTNAGG 1 cut(s) 795
BsuI GTATCC 1 cut(s) 2014
BsuRI GGCC 4 cut(s) 117, 1376, 1563, 2234
BsuTUI ATCGAT 1 cut(s) 781
BtgI CCRYGG 2 cut(s) 1937, 2062
BtsCI GGATG 5 cut(s) 66, 295, 1060, 1947, 2395
BtsI GCAGTG 3 cut(s) 445, 932, 1597
Cac8I GCNNGC 6 cut(s) 205, 914, 1068, 1492, 1528, 2036
CciI TCATGA 1 cut(s) 2068
CfoI GCGC 1 cut(s) 2291
Cfr10I RCCGGY 1 cut(s) 1799
Cfr13I GGNCC 4 cut(s) 799, 1475, 1648, 1797
ClaI ATCGAT 1 cut(s) 781
CseI GACGC 2 cut(s) 1523, 1532
Csp6I GTAC 5 cut(s) 1149, 1730, 1934, 2099, 2129
CspAI ACCGGT 1 cut(s) 1799
CviQI GTAC 5 cut(s) 1149, 1730, 1934, 2099, 2129
DpnI GATC 9 cut(s) 27, 62, 534, 1049, 1500, 1569, 1585, 1815, 1986
DpnII GATC 9 cut(s) 25, 60, 532, 1047, 1498, 1567, 1583, 1813, 1984
DraI TTTAAA 1 cut(s) 1749
DraIII CACNNNGTG 1 cut(s) 2359
Eam1104I CTCTTC 2 cut(s) 2022, 2349
EarI CTCTTC 2 cut(s) 2022, 2349
Ecl136II GAGCTC 1 cut(s) 1597
Eco130I CCWWGG 5 cut(s) 578, 802, 1660, 1937, 2062
Eco24I GRGCYC 3 cut(s) 45, 155, 1599
Eco31I GGTCTC 1 cut(s) 2276
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 4 cut(s) 799, 1475, 1648, 1797
Eco53kI GAGCTC 1 cut(s) 1597
Eco57I CTGAAG 1 cut(s) 1035
Eco81I CCTNAGG 1 cut(s) 795
EcoICRI GAGCTC 1 cut(s) 1597
EcoNI CCTNNNNNAGG 1 cut(s) 681
EcoO109I RGGNCCY 1 cut(s) 799
EcoRI GAATTC 2 cut(s) 2044, 2363
EcoRII CCWGG 6 cut(s) 492, 1050, 1107, 1563, 1688, 2234
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 5 cut(s) 578, 802, 1660, 1937, 2062
EcoT22I ATGCAT 3 cut(s) 1060, 1192, 1492
EcoT38I GRGCYC 3 cut(s) 45, 155, 1599
ErhI CCWWGG 5 cut(s) 578, 802, 1660, 1937, 2062
Esp3I CGTCTC 2 cut(s) 100, 1506
FaqI GGGAC 3 cut(s) 903, 1442, 1461
FauI CCCGC 2 cut(s) 1059, 1595
FauNDI CATATG 1 cut(s) 169
FblI GTMKAC 1 cut(s) 808
Fnu4HI GCNGC 5 cut(s) 50, 174, 452, 1064, 1071
FokI GGATG 5 cut(s) 53, 302, 1067, 1954, 2402
FriOI GRGCYC 3 cut(s) 45, 155, 1599
Fsp4HI GCNGC 5 cut(s) 50, 174, 452, 1064, 1071
FspBI CTAG 5 cut(s) 579, 624, 803, 1661, 1862
GlaI GCGC 1 cut(s) 2290
GluI GCNGC 5 cut(s) 50, 174, 452, 1064, 1071
GsuI CTGGAG 2 cut(s) 107, 747
HaeIII GGCC 4 cut(s) 117, 1376, 1563, 2234
HapII CCGG 1 cut(s) 1800
HgaI GACGC 2 cut(s) 1523, 1532
HhaI GCGC 1 cut(s) 2291
Hin1I GRCGYC 1 cut(s) 279
Hin6I GCGC 1 cut(s) 2289
HinP1I GCGC 1 cut(s) 2289
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 3 cut(s) 392, 1337, 1352
HinfI GANTC 7 cut(s) 77, 467, 488, 607, 661, 1024, 1079
HpaII CCGG 1 cut(s) 1800
HphI GGTGA 8 cut(s) 77, 82, 313, 323, 425, 952, 1286, 1877
Hpy166II GTNNAC 4 cut(s) 277, 314, 809, 1797
Hpy8I GTNNAC 4 cut(s) 277, 314, 809, 1797
Hpy99I CGWCG 1 cut(s) 2435
HpyAV CCTTC 7 cut(s) 264, 685, 940, 1010, 2171, 2345, 2378
HpyCH4III ACNGT 6 cut(s) 94, 652, 1470, 1754, 1897, 2296
HpyCH4IV ACGT 7 cut(s) 214, 279, 364, 633, 892, 1392, 1824
HpyF10VI GCNNNNNNNGC 8 cut(s) 49, 114, 200, 773, 922, 1223, 1523, 1928
HpySE526I ACGT 7 cut(s) 214, 279, 364, 633, 892, 1392, 1824
Hsp92I GRCGYC 1 cut(s) 279
HspAI GCGC 1 cut(s) 2289
Kzo9I GATC 9 cut(s) 25, 60, 532, 1047, 1498, 1567, 1583, 1813, 1984
LguI GCTCTTC 1 cut(s) 2349
LmnI GCTCC 6 cut(s) 40, 226, 773, 1097, 1602, 2135
Lsp1109I GCAGC 5 cut(s) 61, 160, 463, 1075, 1082
LweI GCATC 7 cut(s) 754, 1045, 1046, 1177, 1932, 1999, 2047
MaeI CTAG 5 cut(s) 579, 624, 803, 1661, 1862
MaeII ACGT 7 cut(s) 214, 279, 364, 633, 892, 1392, 1824
MaeIII GTNAC 7 cut(s) 629, 667, 991, 1240, 1299, 1388, 2393
MalI GATC 9 cut(s) 27, 62, 534, 1049, 1500, 1569, 1585, 1815, 1986
MbiI CCGCTC 1 cut(s) 83
MboI GATC 9 cut(s) 25, 60, 532, 1047, 1498, 1567, 1583, 1813, 1984
MboII GAAGA 7 cut(s) 852, 1158, 1441, 1630, 1981, 2039, 2336
MfeI CAATTG 1 cut(s) 2248
MflI RGATCY 5 cut(s) 25, 532, 1047, 1567, 1583
MhlI GDGCHC 3 cut(s) 45, 155, 1599
MlyI GAGTC 3 cut(s) 86, 1033, 1073
MmeI TCCRAC 3 cut(s) 18, 853, 1075
Mph1103I ATGCAT 3 cut(s) 1060, 1192, 1492
MroXI GAANNNNTTC 1 cut(s) 844
MseI TTAA 9 cut(s) 183, 222, 549, 753, 972, 1319, 1536, 1748, 2177
MslI CAYNNNNRTG 3 cut(s) 1370, 1784, 2067
MspA1I CMGCKG 2 cut(s) 1066, 1526
MspI CCGG 1 cut(s) 1800
MspR9I CCNGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
MunI CAATTG 1 cut(s) 2248
MvaI CCWGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
MwoI GCNNNNNNNGC 8 cut(s) 49, 114, 200, 773, 922, 1223, 1523, 1928
NcoI CCATGG 2 cut(s) 1937, 2062
NdeI CATATG 1 cut(s) 169
NdeII GATC 9 cut(s) 25, 60, 532, 1047, 1498, 1567, 1583, 1813, 1984
NlaIV GGNNCC 8 cut(s) 42, 534, 932, 1131, 1477, 1569, 1585, 1721
NmeAIII GCCGAG 2 cut(s) 84, 93
NmuCI GTSAC 2 cut(s) 667, 1240
NsiI ATGCAT 3 cut(s) 1060, 1192, 1492
NspI RCATGY 3 cut(s) 979, 1494, 1840
NspV TTCGAA 2 cut(s) 344, 1699
PaeI GCATGC 1 cut(s) 1494
PagI TCATGA 1 cut(s) 2068
PciI ACATGT 1 cut(s) 1836
PciSI GCTCTTC 1 cut(s) 2349
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 844
PfeI GAWTC 4 cut(s) 467, 488, 607, 661
PflFI GACNNNGTC 1 cut(s) 677
PfoI TCCNGGA 1 cut(s) 1050
PinAI ACCGGT 1 cut(s) 1799
PkrI GCNGC 5 cut(s) 51, 175, 453, 1065, 1072
PleI GAGTC 3 cut(s) 85, 1032, 1073
PpsI GAGTC 3 cut(s) 85, 1032, 1073
Ppu21I YACGTR 2 cut(s) 1393, 1825
PpuMI RGGWCCY 1 cut(s) 799
PscI ACATGT 1 cut(s) 1836
Psp124BI GAGCTC 1 cut(s) 1599
Psp1406I AACGTT 2 cut(s) 364, 633
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 6 cut(s) 492, 1050, 1107, 1563, 1688, 2234
PspGI CCWGG 6 cut(s) 492, 1050, 1107, 1563, 1688, 2234
PspN4I GGNNCC 8 cut(s) 42, 534, 932, 1131, 1477, 1569, 1585, 1721
PspPI GGNCC 4 cut(s) 799, 1475, 1648, 1797
PspPPI RGGWCCY 1 cut(s) 799
PsuI RGATCY 5 cut(s) 25, 532, 1047, 1567, 1583
PsyI GACNNNGTC 1 cut(s) 677
PvuII CAGCTG 1 cut(s) 1526
RsaI GTAC 5 cut(s) 1150, 1731, 1935, 2100, 2130
RsaNI GTAC 5 cut(s) 1149, 1730, 1934, 2099, 2129
RseI CAYNNNNRTG 3 cut(s) 1370, 1784, 2067
SacI GAGCTC 1 cut(s) 1599
SapI GCTCTTC 1 cut(s) 2349
SaqAI TTAA 9 cut(s) 183, 222, 549, 753, 972, 1319, 1536, 1748, 2177
SatI GCNGC 5 cut(s) 50, 174, 452, 1064, 1071
Sau3AI GATC 9 cut(s) 25, 60, 532, 1047, 1498, 1567, 1583, 1813, 1984
Sau96I GGNCC 4 cut(s) 799, 1475, 1648, 1797
SchI GAGTC 3 cut(s) 86, 1033, 1073
ScrFI CCNGG 6 cut(s) 494, 1052, 1109, 1565, 1690, 2236
SduI GDGCHC 3 cut(s) 45, 155, 1599
SfaNI GCATC 7 cut(s) 754, 1045, 1046, 1177, 1932, 1999, 2047
SfcI CTRYAG 3 cut(s) 899, 1521, 1851
SfuI TTCGAA 2 cut(s) 344, 1699
SinI GGWCC 4 cut(s) 799, 1475, 1648, 1797
SmiMI CAYNNNNRTG 3 cut(s) 1370, 1784, 2067
SmlI CTYRAG 3 cut(s) 296, 317, 2015
SmoI CTYRAG 3 cut(s) 296, 317, 2015
SphI GCATGC 1 cut(s) 1494
SsiI CCGC 3 cut(s) 81, 1066, 1588
SspMI CTAG 5 cut(s) 579, 624, 803, 1661, 1862
SstI GAGCTC 1 cut(s) 1599
StyD4I CCNGG 6 cut(s) 492, 1050, 1107, 1563, 1688, 2234
StyI CCWWGG 5 cut(s) 578, 802, 1660, 1937, 2062
TaaI ACNGT 6 cut(s) 94, 652, 1470, 1754, 1897, 2296
TaiI ACGT 7 cut(s) 217, 282, 367, 636, 895, 1395, 1827
TaqI TCGA 5 cut(s) 344, 616, 781, 1699, 2347
TatI WGTACW 1 cut(s) 2128
TfiI GAWTC 4 cut(s) 467, 488, 607, 661
Tru1I TTAA 9 cut(s) 183, 222, 549, 753, 972, 1319, 1536, 1748, 2177
Tru9I TTAA 9 cut(s) 183, 222, 549, 753, 972, 1319, 1536, 1748, 2177
TseFI GTSAC 2 cut(s) 667, 1240
TseI GCWGC 5 cut(s) 49, 173, 451, 1063, 1070
Tsp45I GTSAC 2 cut(s) 667, 1240
TspGWI ACGGA 2 cut(s) 545, 1136
Tth111I GACNNNGTC 1 cut(s) 677
VpaK11BI GGWCC 4 cut(s) 799, 1475, 1648, 1797
XagI CCTNNNNNAGG 1 cut(s) 681
XapI RAATTY 5 cut(s) 340, 1157, 2044, 2123, 2363
XceI RCATGY 3 cut(s) 979, 1494, 1840
XcmI CCANNNNNNNNNTGG 1 cut(s) 2060
XmaJI CCTAGG 3 cut(s) 578, 802, 1660
XmiI GTMKAC 1 cut(s) 808
XmnI GAANNNNTTC 1 cut(s) 844
XspI CTAG 5 cut(s) 579, 624, 803, 1661, 1862
ZraI GACGTC 1 cut(s) 280
Zsp2I ATGCAT 3 cut(s) 1060, 1192, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.