AT4G39080

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003618
Physical Location & Seq
Forward (+)
18209399 .. 18215204
5806 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G39080.1

Sequence Viewer

Length: 2466 bp
ATGGCGGAAAGTGGCGGTGGCGGTGGCTGTTGTCCGCCGATGGATCTGATGCGTTCAGAGACGATGCAACTTGTTCAGCTCATTGTTCCCATGGAATCTGCTCATCTCACCGTCTCTTACCTTGGAGATCTTGGCCTCGTCCAATTCAAAGACCTTAATTCAGAGAAGAGCCCATTTCAACGTACTTACGCAGCACAGATTAAAAGATGTGGAGAAATGGCACGGAAAATACGTTTCTTCAGGGATCAAATGTCAAAAGCAGGAGTTCCAGCCAAGGAAATGCAAGGGAAAGAAAATGACATTGACTTGGATGATGTTGAGGTAAAGCTTGGAGAGCTAGAAGCTGAACTTGTTGAAATCAATGCTAATAATGACAAGTTGCAGCGCTCCTATAATGAGCTTATGGAGTACAAGTTGGTTCTTCAGAAGGCTGGTGAGTTCTTTTCTTCTGCTCATAGAAGTGCCGCAGATCAACAGAGAGAGACAGAATCACAACAAGCGGGTGAAGATCTGCTGGAGTCTCCTTTGTTACAGGAAGAGAAGTCCATTGATTCGACAAAGCAAGTAAAGCTTGGATTTCTTACCGGATTAGTGCCTCGTGAAAAGTCTATGGTCTTTGAGAGGATTCTGTTCCGTGCGACCAGGGGCAACATTTTTATCCGACAGACTGTCATTGAGGAGCCAGTCATTGATCCCAACTCTGGGGAGAAGGCTGAGAAAAATGTTTTTGTTGTCTTCTATTCCGGGGAAAGAGCGAAAAGCAAAATTCTGAAAATATGTGAAGCTTTTGGGGCCAATCGTTATCCTTTCAGTGAGGACCTTGGAAGACAAGCTCAGATGATAACCGAGGTTTCTGGTCGATTATCCGAGCTTAAAACCACGATAGATGCTGGGTTAGGTCAGCGTAACATTCTTTTGCAGACCATTGGAGATAAGTTTGAACTATGGAACCTCAAGGTACGCAAAGAGAAAGCTATCTATCATACATTGAACATGCTTAGTCTTGATGTGACGAAGAAGTGCCTCGTGGCCGAGGGCTGGAGTCCTGTCTTTGCATCAAGAGAAATTCAAGATGCATTACAGCGTGCCGCCGTTGACTCCAATTCTCAAGTTGGATCAATTTTCCAGGTCCTAAGAACCAAAGAGTCGCCACCAACTTATTTTCGCACAAACAAATTTACTTCTGCAATCCAGGAAATTGTTGATGCGTATGGTGTGGCCAAGTATCAGGAAGCCAATCCAGGTGTATTCACCATTGTTACCTTCCCCTTCCTTTTCGCTGTTATGTTTGGTGATTGGGGGCATGGAATATGCATTCTGCTTGCAACTATGTACTTGATATTGAAAGAAAAGAAACTTGCCAGCCAGAAACTTGGGGATATTATGGAAATGGCTTTTGGTGGGCGTTATGTCATTTTGATGATGTCACTCTTCTCAATATACACTGGATTAATCTACAACGAGTTCTTCTCTATACCATTCCCATTGTTTGCTCCTTCAGCGTATGATTGCCGGGATGTCTCTTGCAGCGAAGCTACAACAATTGGTTTGATAAAGGTCAGAGACACTTATCCATTTGGTCTAGATCCTGTGTGGCATGGATCCCGCAGTGAGCTGCCATTCCTGAACTCGCTGAAGATGAAAATGTCAATCCTTCTCGGAGTTTCTCAAATGAACCTCGGAATCATAATGAGCTACTTTAATGCTAGATTCTTCAAATCAAGTGTAAATATATGGTTCCAGTTCATTCCCCAAATGATATTCTTGAACAGCTTGTTTGGCTATCTCTCAGTCCTCATCATCATAAAGTGGTGCACTGGTTCTCAAGCAGATTTGTATCATGTAATGATATATATGTTCCTGAGCCCAATGGACGAGTTAGGAGAGAATCAACTGTTCCCTCACCAGAAAACATTACAGCTTGTGCTTCTCTTTCTGGCTCTTGTTTCTGTTCCGTGTATGTTGCTTCCAAAGCCGTTCATCTTGAAGAAACAACATGAAGCTAGGCATCAAGGCCAGGCGTACGCACCTCTTGACGAGACAGATGAGAGTCTTCATGTAGAGACAAACGGAGGAGGATCCCATGGGCATGAGGAGTTTGAATTCAGCGAGATATTTGTGCATCAGCTGATTCACACCATAGAGTTTGTGCTTGGAGCTGTTTCCAACACCGCTTCTTACCTGCGTCTATGGGCTCTCAGTCTTGCCCACTCGGAGCTGTCATCAGTCTTCTATGAGAAGGTCCTCCTTCTCGCTTGGGGTTACAACAATCCGTTGATCCTGATCGTTGGGGTCTTGGTGTTCATATTCGCAACCGTGGGAGTTTTGCTGGTGATGGAGACACTAAGCGCGTTCCTTCACGCGCTGCGTCTTCACTGGGTTGAGTTTCAGAACAAATTCTACGAAGGAGATGGTTACAAGTTCGCTCCTTTCACTTTCATTTTCACGGCAAACGAAGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

821

Amino Acids

92.83

Weight (kDa)

5.66

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 40 - 813 0.0 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2190
AccII CGCG 2 cut(s) 2351, 2363
AciI CCGC 9 cut(s) 5, 15, 21, 35, 465, 500, 1089, 1606, 2172
AcoI YGGCCR 2 cut(s) 1029, 1218
AcsI RAATTY 5 cut(s) 765, 1065, 1175, 2102, 2396
AcuI CTGAAG 4 cut(s) 223, 407, 1482, 1655
AfaI GTAC 5 cut(s) 184, 410, 960, 1334, 2024
AfeI AGCGCT 1 cut(s) 386
AfiI CCNNNNNNNGG 3 cut(s) 701, 702, 1038
AhdI GACNNNNNGTC 1 cut(s) 668
AjnI CCWGG 5 cut(s) 641, 1125, 1191, 1240, 2016
AjuI GAANNNNNNNTTGG 2 cut(s) 1380, 1412
Alw21I GWGCWC 1 cut(s) 1817
Alw26I GTCTC 9 cut(s) 53, 118, 476, 525, 1525, 1557, 2033, 2057, 2333
Alw44I GTGCAC 1 cut(s) 1813
Aor51HI AGCGCT 1 cut(s) 386
AoxI GGCC 5 cut(s) 133, 792, 1029, 1218, 2014
ApaLI GTGCAC 1 cut(s) 1813
ApeKI GCWGC 5 cut(s) 191, 382, 1527, 1615, 2365
ApoI RAATTY 5 cut(s) 765, 1065, 1175, 2102, 2396
AseI ATTAAT 1 cut(s) 1451
Asp700I GAANNNNTTC 1 cut(s) 2396
AspLEI GCGC 3 cut(s) 387, 2351, 2365
AspS9I GGNCC 4 cut(s) 792, 817, 1129, 2242
AsuC2I CCSGG 2 cut(s) 745, 1514
AsuHPI GGTGA 7 cut(s) 100, 446, 515, 1243, 1304, 1895, 2344
AvaII GGWCC 3 cut(s) 817, 1129, 2242
BaeGI GKGCMC 1 cut(s) 1817
BalI TGGCCA 1 cut(s) 1220
BamHI GGATCC 2 cut(s) 1601, 2078
BanII GRGCYC 3 cut(s) 173, 1868, 2197
BauI CACGAG 2 cut(s) 597, 1025
BbsI GAAGAC 5 cut(s) 727, 832, 2045, 2221, 2363
Bbv12I GWGCWC 1 cut(s) 1817
BbvI GCAGC 5 cut(s) 203, 394, 1539, 1602, 2352
BccI CCATC 3 cut(s) 34, 2329, 2405
BceAI ACGGC 3 cut(s) 1076, 1960, 2463
BciT130I CCWGG 5 cut(s) 643, 1127, 1193, 1242, 2018
BcnI CCSGG 2 cut(s) 745, 1514
BcoDI GTCTC 9 cut(s) 53, 118, 476, 525, 1525, 1557, 2033, 2057, 2333
BfaI CTAG 4 cut(s) 338, 1583, 1707, 2004
BfoI RGCGCY 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 2190
BglII AGATCT 2 cut(s) 127, 508
BisI GCNGC 7 cut(s) 192, 383, 465, 1089, 1528, 1616, 2366
BlsI GCNGC 7 cut(s) 193, 384, 466, 1090, 1529, 1617, 2367
Bme1390I CCNGG 7 cut(s) 643, 745, 1127, 1193, 1242, 1514, 2018
Bme18I GGWCC 3 cut(s) 817, 1129, 2242
BmeRI GACNNNNNGTC 1 cut(s) 668
BmgT120I GGNCC 4 cut(s) 792, 817, 1129, 2242
BmiI GGNNCC 6 cut(s) 681, 793, 950, 1603, 1739, 2080
BmrFI CCNGG 7 cut(s) 643, 745, 1127, 1193, 1242, 1514, 2018
BmrI ACTGGG 1 cut(s) 2386
BmsI GCATC 8 cut(s) 39, 54, 877, 1063, 1064, 1195, 2017, 2131
BmuI ACTGGG 1 cut(s) 2386
BpiI GAAGAC 5 cut(s) 727, 832, 2045, 2221, 2363
BplI GAGNNNNNCTC 2 cut(s) 1454, 1486
BpmI CTGGAG 2 cut(s) 536, 1060
Bpu10I CCTNAGC 1 cut(s) 1862
BpuEI CTTGAG 3 cut(s) 938, 1092, 1809
BpuMI CCSGG 2 cut(s) 745, 1514
BsaBI GATNNNNATC 2 cut(s) 1836, 2282
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 3 cut(s) 701, 702, 1038
Bse1I ACTGG 5 cut(s) 683, 1450, 1741, 1822, 2381
Bse8I GATNNNNATC 2 cut(s) 1836, 2282
BseBI CCWGG 5 cut(s) 643, 1127, 1193, 1242, 2018
BseGI GGATG 2 cut(s) 316, 1522
BseJI GATNNNNATC 2 cut(s) 1836, 2282
BseLI CCNNNNNNNGG 3 cut(s) 701, 702, 1038
BseMII CTCAG 5 cut(s) 705, 848, 1803, 1853, 2212
BseNI ACTGG 5 cut(s) 683, 1450, 1741, 1822, 2381
BseRI GAGGAG 3 cut(s) 692, 2088, 2108
BseSI GKGCMC 1 cut(s) 1817
BseXI GCAGC 5 cut(s) 203, 394, 1539, 1602, 2352
BseYI CCCAGC 1 cut(s) 890
Bsh1236I CGCG 2 cut(s) 2351, 2363
BshFI GGCC 5 cut(s) 135, 794, 1031, 1220, 2016
BsiHKAI GWGCWC 1 cut(s) 1817
BsiSI CCGG 3 cut(s) 585, 744, 1513
BsiWI CGTACG 1 cut(s) 2022
BslI CCNNNNNNNGG 3 cut(s) 701, 702, 1038
BsmAI GTCTC 9 cut(s) 53, 118, 476, 525, 1525, 1557, 2033, 2057, 2333
BsmBI CGTCTC 2 cut(s) 53, 118
BsmI GAATGC 1 cut(s) 1314
BsnI GGCC 5 cut(s) 135, 794, 1031, 1220, 2016
Bsp1286I GDGCHC 4 cut(s) 173, 1817, 1868, 2197
Bsp19I CCATGG 2 cut(s) 90, 2083
BspACI CCGC 9 cut(s) 5, 15, 21, 35, 465, 500, 1089, 1606, 2172
BspANI GGCC 5 cut(s) 135, 794, 1031, 1220, 2016
BspCNI CTCAG 5 cut(s) 706, 847, 1802, 1854, 2211
BspFNI CGCG 2 cut(s) 2351, 2363
BspLI GGNNCC 6 cut(s) 681, 793, 950, 1603, 1739, 2080
BspMI ACCTGC 1 cut(s) 2190
BspQI GCTCTTC 1 cut(s) 161
BsrI ACTGG 5 cut(s) 683, 1450, 1741, 1822, 2381
BssSI CACGAG 2 cut(s) 597, 1025
BssT1I CCWWGG 5 cut(s) 90, 121, 273, 820, 2083
Bst2BI CACGAG 2 cut(s) 597, 1025
Bst2UI CCWGG 5 cut(s) 643, 1127, 1193, 1242, 2018
Bst4CI ACNGT 4 cut(s) 112, 670, 1896, 2317
Bst6I CTCTTC 3 cut(s) 161, 531, 1436
BstC8I GCNNGC 3 cut(s) 1086, 1323, 1363
BstDEI CTNAG 8 cut(s) 714, 834, 998, 1133, 1789, 1862, 2198, 2345
BstDSI CCRYGG 3 cut(s) 90, 2083, 2316
BstF5I GGATG 2 cut(s) 316, 1522
BstFNI CGCG 2 cut(s) 2351, 2363
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 3 cut(s) 387, 2351, 2365
BstMAI GTCTC 9 cut(s) 53, 118, 476, 525, 1525, 1557, 2033, 2057, 2333
BstMWI GCNNNNNNNGC 6 cut(s) 334, 568, 791, 1499, 1779, 1972
BstNI CCWGG 5 cut(s) 643, 1127, 1193, 1242, 2018
BstNSI RCATGY 1 cut(s) 997
BstSCI CCNGG 7 cut(s) 641, 743, 1125, 1191, 1240, 1512, 2016
BstSLI GKGCMC 1 cut(s) 1817
BstUI CGCG 2 cut(s) 2351, 2363
BstV1I GCAGC 5 cut(s) 203, 394, 1539, 1602, 2352
BstV2I GAAGAC 5 cut(s) 727, 832, 2045, 2221, 2363
BstX2I RGATCY 6 cut(s) 43, 127, 508, 1585, 1601, 2078
BstXI CCANNNNNNTGG 1 cut(s) 1373
BstYI RGATCY 6 cut(s) 43, 127, 508, 1585, 1601, 2078
BsuRI GGCC 5 cut(s) 135, 794, 1031, 1220, 2016
BtgI CCRYGG 3 cut(s) 90, 2083, 2316
BtsCI GGATG 2 cut(s) 316, 1522
BtsI GCAGTG 1 cut(s) 1615
BtsIMutI CAGTG 5 cut(s) 817, 1443, 1615, 1815, 2374
BveI ACCTGC 1 cut(s) 2190
Cac8I GCNNGC 3 cut(s) 1086, 1323, 1363
CfoI GCGC 3 cut(s) 387, 2351, 2365
Cfr13I GGNCC 4 cut(s) 792, 817, 1129, 2242
CseI GACGC 2 cut(s) 2174, 2357
Csp6I GTAC 5 cut(s) 183, 409, 959, 1333, 2023
CviAII CATG 9 cut(s) 91, 994, 1304, 1598, 1841, 1997, 2057, 2084, 2090
CviQI GTAC 5 cut(s) 183, 409, 959, 1333, 2023
DdeI CTNAG 8 cut(s) 714, 834, 998, 1133, 1789, 1862, 2198, 2345
DriI GACNNNNNGTC 1 cut(s) 668
EaeI YGGCCR 2 cut(s) 1029, 1218
Eam1104I CTCTTC 3 cut(s) 161, 531, 1436
Eam1105I GACNNNNNGTC 1 cut(s) 668
EarI CTCTTC 3 cut(s) 161, 531, 1436
EciI GGCGGA 2 cut(s) 20, 24
Eco130I CCWWGG 5 cut(s) 90, 121, 273, 820, 2083
Eco24I GRGCYC 3 cut(s) 173, 1868, 2197
Eco47I GGWCC 3 cut(s) 817, 1129, 2242
Eco47III AGCGCT 1 cut(s) 386
Eco57I CTGAAG 4 cut(s) 223, 407, 1482, 1655
EcoO109I RGGNCCY 3 cut(s) 817, 1129, 2242
EcoRI GAATTC 1 cut(s) 2102
EcoRII CCWGG 5 cut(s) 641, 1125, 1191, 1240, 2016
EcoT14I CCWWGG 5 cut(s) 90, 121, 273, 820, 2083
EcoT22I ATGCAT 2 cut(s) 1078, 1316
EcoT38I GRGCYC 3 cut(s) 173, 1868, 2197
ErhI CCWWGG 5 cut(s) 90, 121, 273, 820, 2083
Esp3I CGTCTC 2 cut(s) 53, 118
FaeI CATG 9 cut(s) 94, 997, 1307, 1601, 1844, 2000, 2060, 2087, 2093
FalI AAGNNNNNCTT 4 cut(s) 333, 365, 555, 587
FatI CATG 9 cut(s) 90, 993, 1303, 1597, 1840, 1996, 2056, 2083, 2089
FauI CCCGC 2 cut(s) 493, 1613
Fnu4HI GCNGC 7 cut(s) 192, 383, 465, 1089, 1528, 1616, 2366
FokI GGATG 2 cut(s) 323, 1529
FriOI GRGCYC 3 cut(s) 173, 1868, 2197
Fsp4HI GCNGC 7 cut(s) 192, 383, 465, 1089, 1528, 1616, 2366
FspBI CTAG 4 cut(s) 338, 1583, 1707, 2004
GlaI GCGC 3 cut(s) 386, 2350, 2364
GluI GCNGC 7 cut(s) 192, 383, 465, 1089, 1528, 1616, 2366
GsaI CCCAGC 1 cut(s) 894
GsuI CTGGAG 2 cut(s) 536, 1060
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 5 cut(s) 135, 794, 1031, 1220, 2016
HapII CCGG 3 cut(s) 585, 744, 1513
HgaI GACGC 2 cut(s) 2174, 2357
HhaI GCGC 3 cut(s) 387, 2351, 2365
Hin1II CATG 9 cut(s) 94, 997, 1307, 1601, 1844, 2000, 2060, 2087, 2093
Hin6I GCGC 3 cut(s) 385, 2349, 2363
HinP1I GCGC 3 cut(s) 385, 2349, 2363
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 3 cut(s) 326, 569, 783
HpaII CCGG 3 cut(s) 585, 744, 1513
HphI GGTGA 7 cut(s) 100, 446, 515, 1243, 1304, 1895, 2344
Hpy166II GTNNAC 2 cut(s) 1096, 1815
Hpy8I GTNNAC 2 cut(s) 1096, 1815
HpyCH4III ACNGT 4 cut(s) 112, 670, 1896, 2317
HpyCH4IV ACGT 2 cut(s) 181, 232
HpyF10VI GCNNNNNNNGC 6 cut(s) 334, 568, 791, 1499, 1779, 1972
HpyF3I CTNAG 8 cut(s) 714, 834, 998, 1133, 1789, 1862, 2198, 2345
HpySE526I ACGT 2 cut(s) 181, 232
Hsp92II CATG 9 cut(s) 94, 997, 1307, 1601, 1844, 2000, 2060, 2087, 2093
HspAI GCGC 3 cut(s) 385, 2349, 2363
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 6 cut(s) 392, 679, 1498, 2156, 2215, 2431
Lsp1109I GCAGC 5 cut(s) 203, 394, 1539, 1602, 2352
LweI GCATC 8 cut(s) 39, 54, 877, 1063, 1064, 1195, 2017, 2131
MaeI CTAG 4 cut(s) 338, 1583, 1707, 2004
MaeII ACGT 2 cut(s) 181, 232
MaeIII GTNAC 7 cut(s) 528, 905, 1009, 1258, 1425, 2261, 2414
MfeI CAATTG 1 cut(s) 1542
MflI RGATCY 6 cut(s) 43, 127, 508, 1585, 1601, 2078
MhlI GDGCHC 4 cut(s) 173, 1817, 1868, 2197
MlsI TGGCCA 1 cut(s) 1220
MluNI TGGCCA 1 cut(s) 1220
MlyI GAGTC 5 cut(s) 527, 1051, 1091, 1154, 2059
MmeI TCCRAC 3 cut(s) 685, 1093, 2190
Mox20I TGGCCA 1 cut(s) 1220
Mph1103I ATGCAT 2 cut(s) 1078, 1316
MroXI GAANNNNTTC 1 cut(s) 2396
MscI TGGCCA 1 cut(s) 1220
MseI TTAA 5 cut(s) 156, 201, 873, 1451, 1701
MslI CAYNNNNRTG 3 cut(s) 459, 1418, 2088
Msp20I TGGCCA 1 cut(s) 1220
MspA1I CMGCKG 1 cut(s) 2128
MspI CCGG 3 cut(s) 585, 744, 1513
MspR9I CCNGG 7 cut(s) 643, 745, 1127, 1193, 1242, 1514, 2018
MunI CAATTG 1 cut(s) 1542
Mva1269I GAATGC 1 cut(s) 1314
MvaI CCWGG 5 cut(s) 643, 1127, 1193, 1242, 2018
MvnI CGCG 2 cut(s) 2351, 2363
MwoI GCNNNNNNNGC 6 cut(s) 334, 568, 791, 1499, 1779, 1972
NciI CCSGG 2 cut(s) 745, 1514
NcoI CCATGG 2 cut(s) 90, 2083
NlaIII CATG 9 cut(s) 94, 997, 1307, 1601, 1844, 2000, 2060, 2087, 2093
NlaIV GGNNCC 6 cut(s) 681, 793, 950, 1603, 1739, 2080
NmeAIII GCCGAG 1 cut(s) 1057
NmuCI GTSAC 2 cut(s) 1009, 1425
NsiI ATGCAT 2 cut(s) 1078, 1316
NspI RCATGY 1 cut(s) 997
PciSI GCTCTTC 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 229
PctI GAATGC 1 cut(s) 1314
PdmI GAANNNNTTC 1 cut(s) 2396
PfeI GAWTC 8 cut(s) 95, 488, 551, 625, 1683, 1710, 1888, 2131
Pfl23II CGTACG 1 cut(s) 2022
PfoI TCCNGGA 1 cut(s) 1191
PkrI GCNGC 7 cut(s) 193, 384, 466, 1090, 1529, 1617, 2367
PleI GAGTC 5 cut(s) 526, 1050, 1091, 1153, 2058
PpsI GAGTC 5 cut(s) 526, 1050, 1091, 1153, 2058
PpuMI RGGWCCY 3 cut(s) 817, 1129, 2242
PshBI ATTAAT 1 cut(s) 1451
Psp5II RGGWCCY 3 cut(s) 817, 1129, 2242
Psp6I CCWGG 5 cut(s) 641, 1125, 1191, 1240, 2016
PspFI CCCAGC 1 cut(s) 890
PspGI CCWGG 5 cut(s) 641, 1125, 1191, 1240, 2016
PspLI CGTACG 1 cut(s) 2022
PspN4I GGNNCC 6 cut(s) 681, 793, 950, 1603, 1739, 2080
PspPI GGNCC 4 cut(s) 792, 817, 1129, 2242
PspPPI RGGWCCY 3 cut(s) 817, 1129, 2242
PsrI GAACNNNNNNTAC 2 cut(s) 2384, 2416
PsuI RGATCY 6 cut(s) 43, 127, 508, 1585, 1601, 2078
PvuII CAGCTG 1 cut(s) 2128
RsaI GTAC 5 cut(s) 184, 410, 960, 1334, 2024
RsaNI GTAC 5 cut(s) 183, 409, 959, 1333, 2023
RseI CAYNNNNRTG 3 cut(s) 459, 1418, 2088
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 5 cut(s) 156, 201, 873, 1451, 1701
SatI GCNGC 7 cut(s) 192, 383, 465, 1089, 1528, 1616, 2366
Sau96I GGNCC 4 cut(s) 792, 817, 1129, 2242
SchI GAGTC 5 cut(s) 527, 1051, 1091, 1154, 2059
ScrFI CCNGG 7 cut(s) 643, 745, 1127, 1193, 1242, 1514, 2018
SduI GDGCHC 4 cut(s) 173, 1817, 1868, 2197
SfaNI GCATC 8 cut(s) 39, 54, 877, 1063, 1064, 1195, 2017, 2131
SinI GGWCC 3 cut(s) 817, 1129, 2242
SmiMI CAYNNNNRTG 3 cut(s) 459, 1418, 2088
SmlI CTYRAG 3 cut(s) 953, 1107, 1824
SmoI CTYRAG 3 cut(s) 953, 1107, 1824
SsiI CCGC 9 cut(s) 5, 15, 21, 35, 465, 500, 1089, 1606, 2172
SspMI CTAG 4 cut(s) 338, 1583, 1707, 2004
StyD4I CCNGG 7 cut(s) 641, 743, 1125, 1191, 1240, 1512, 2016
StyI CCWWGG 5 cut(s) 90, 121, 273, 820, 2083
TaaI ACNGT 4 cut(s) 112, 670, 1896, 2317
TaiI ACGT 2 cut(s) 184, 235
TaqI TCGA 2 cut(s) 554, 859
TatI WGTACW 2 cut(s) 408, 1332
TauI GCSGC 2 cut(s) 467, 1091
TfiI GAWTC 8 cut(s) 95, 488, 551, 625, 1683, 1710, 1888, 2131
Tru1I TTAA 5 cut(s) 156, 201, 873, 1451, 1701
Tru9I TTAA 5 cut(s) 156, 201, 873, 1451, 1701
TscAI CASTG 5 cut(s) 817, 1450, 1615, 1822, 2381
TseFI GTSAC 2 cut(s) 1009, 1425
TseI GCWGC 5 cut(s) 191, 382, 1527, 1615, 2365
Tsp45I GTSAC 2 cut(s) 1009, 1425
TspDTI ATGAA 8 cut(s) 1655, 1688, 1735, 1969, 2013, 2045, 2293, 2428
TspGWI ACGGA 5 cut(s) 238, 623, 1944, 2085, 2262
TspRI CASTG 5 cut(s) 817, 1450, 1615, 1822, 2381
VneI GTGCAC 1 cut(s) 1813
VpaK11BI GGWCC 3 cut(s) 817, 1129, 2242
VspI ATTAAT 1 cut(s) 1451
XapI RAATTY 5 cut(s) 765, 1065, 1175, 2102, 2396
XbaI TCTAGA 1 cut(s) 1582
XceI RCATGY 1 cut(s) 997
XmnI GAANNNNTTC 1 cut(s) 2396
XspI CTAG 4 cut(s) 338, 1583, 1707, 2004
Zsp2I ATGCAT 2 cut(s) 1078, 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.