FvH4_2g38960

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003618
Physical Location & Seq
Forward (+)
28088617 .. 28094473
5857 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38960.t1

Sequence Viewer

Length: 2457 bp
ATGGATGGTTGGCCAACGATGGATCTGTTTCGGTCGGAGCCAATGCAATTGGTGCACCTGATCATTCCGATTGACTCGTCTCGCCGCGCCATCTCCTACCTCGGCGAGCTCGGCCTTTTTCAATTCAAAGATCTCAATGCGGAGAAGAGTCCGTTCCAGAGAACTTACGCCGCTCAGATCAAACGATGCGGGGAAATGGCTAGAAGGTTACGATTCTTTAGAGATCAGATGAGGAAGGCTGGGTTGTTGTCACAATCAACAATGTCGACAATGAGCAATGAAACTGATCTCGATAGCTTGGAGGTTAAACTCGGTGAACTTGAAGGTGATTTGTTAGAAATGAATGCAAATAATGAACAATTGCAACGCACTTATAACGAGCTATTAGAATACAAGCTTGTACTGCAGAAGGCTGGGGAGTTTTTTAGTTCAGCTCAAAGTATTGCCGCAGCTCAGCAGAGAGAAATTGAAGTGCAGCCCATGGGTGAAAGATCCATGGACAGTCCATTATTACTCGAACAAGAAATGACAACAGATCCATCAAAGCATGTAAAGCTTGGGTCCGTGAGTGGTCTTGTTCCAAGAGAAAAATCTATGGCCTTTGAAAGGATTCTATTTCGTGCCACCAGGGGTAATGTATTTCTGAAGCAATCTGTCGTTAATGGTGCTGTTGTTGATCCTGTATCAGGAGAGAAGGTTGAGAAAAATGTATTTATCATCTTCTATTCCGGAGAAAGGGCAAAGAGTAAAATTCTGAAAATATGTGAGGCTTTTGGAGCAAACCGTTACCCATTTACAGATGACTTGGGAAAACAATTTCAGATGATTACTGAGGTGTCTGGAAAAATTTCAGAGCTGAAGTCTACTATTGATGCTGGACTGTTGCATCGGAACAGTTTATTACAGACAATTGGACATCAATATGAACAATGGAATCTTCTGGTGAAGAAGGAAAAATCCATTTACCACACCTTAAATATGCTGAGCATCGATGTGACAAAGATGTGCCTTGTGGCAGAAGGTTGGTGTCCCGTATCAGCGTCACTTCAGATCCAAAATGCACTGCAGCAGGCAACCTACGATAGCAACTCCCAAGTTGGGGCCATATTCCAGGTTTTGCACACAAAGGAATCGCCACCTACATATTTCCGTACAAACAAATTTACTACTGCTTTTCAAGAAATTGTTGATGCATATGGGGTTGCGAAGTATCAGGAGGCTAATCCTGGTGTATATACCATTGTCACTTTCCCTTTCCTTTTTGCTGTGATGTTTGGAGACTGGGGGCATGGTATTTGCTTGTTGCTGGCAACACTATATTTCATCATCAGCGAGAGAAAGTTTTCTAATCAGAAACTTGGGGACATTATTGAAATGACGTTTGGTGGGCGTTACGTTATATTTATGATGGCGCTGTTCTCAATCTACACAGGATTGATATACAATGAATTCTTTTCAGTCCCATTTGAACTGTTTGGCCCTTCTGCCTATGCATGCCGCGACCCATCCTGCAGGGATGCCACAACAGTGGGTTTGACTAAGGTGCGTGACACCTATCCATTTGGTTTGGACCCTAAATGGCATGGTTCCAGGTCCGAGTTACCCTTTCTGAACTCGTTGAAGATGAAGATGTCAATCCTCCTAGGAGTAGCGCAGATGAATCTTGGAATTGTATTAAGTTATGTCAATGCAAAATTTTTTGCTGATAAGCTGAATGTCTGGTACCAATTTGTTCCTCAGATGATATTTCTGAACAGTCTGTTTGGCTACCTTTCGCTCCTAATTATTGTGAAATGGTGCACTGGTTCAAAAGCTGATTTGTATCATGTAATGATATACATGTTCCTGAGTCCTTTCGACGATCTGGGTGAAAACCAGCTCTTTTGGGGGCAAGGATTCCTTCAGGTTCTATTATTACTATCAGCTCTTGTTGCTGTTCCATGGATGCTGTTTCCAAAGCCTTATTTCTTGAAGAAGCAGCATGAGGAGAGGCACCAAGGTCAATCCTACGCGTTGCTTCTGAGTGGGGAGGATCCTCTTGATGATGACCAGGAAGACCATCACAAATCAAAAGATCACGAGGAATTTGAATTCAGCGAGATCTTTGTACACCAACTTATACATACCATAGAATTTGTTCTTGGAGCAGTGTCCAATACAGCTTCATATTTGCGTTTATGGGCACTCAGCTTAGCACATTCAGAGTTGTCCAGTGTATTTTACGACAAGGTTCTACTTCTAGCTTGGGGGTACGACAATGTCATTATTCTAATCATTGGCATAATCGTTTTTATAAGTGCAACTGGTGGAGTGCTACTAGTGATGGAGACCTTGAGTGCGTTCTTGCATGCCTTGAGGCTTCACTGGGTGGAGTTCATGAACAAGTTTTATGAGGGCTCTGGTTACAAATTCTACCCTTTTTCATTTGCATTACTCAGCAGTGAGGACGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

819

Amino Acids

93.12

Weight (kDa)

5.7

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 33 - 809 4.5e-303 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 375, 2294
Acc65I GGTACC 1 cut(s) 1722
AccB1I GGYRCC 2 cut(s) 1722, 1992
AccBSI CCGCTC 1 cut(s) 173
AccI GTMKAC 2 cut(s) 266, 863
AccII CGCG 3 cut(s) 87, 1500, 2012
AccIII TCCGGA 1 cut(s) 728
AciI CCGC 6 cut(s) 85, 140, 171, 189, 447, 1498
AclWI GGATC 7 cut(s) 30, 486, 530, 671, 1045, 2027, 2040
AcoI YGGCCR 1 cut(s) 11
AcsI RAATTY 9 cut(s) 750, 846, 1160, 1448, 1694, 2084, 2090, 2132, 2408
AcuI CTGAAG 4 cut(s) 665, 878, 1031, 1886
AdeI CACNNNGTG 1 cut(s) 2368
AfaI GTAC 5 cut(s) 402, 1153, 1724, 2109, 2252
AfiI CCNNNNNNNGG 5 cut(s) 606, 686, 735, 1098, 1099
AflIII ACRYGT 2 cut(s) 1839, 2010
AhlI ACTAGT 1 cut(s) 2317
AjnI CCWGG 5 cut(s) 626, 1110, 1225, 1589, 2049
AjuI GAANNNNNNNTTGG 4 cut(s) 1365, 1397, 2124, 2156
AleI CACNNNNGTG 1 cut(s) 1526
Alw21I GWGCWC 3 cut(s) 57, 111, 1802
Alw26I GTCTC 3 cut(s) 84, 1272, 2321
Alw44I GTGCAC 2 cut(s) 53, 1798
AlwI GGATC 7 cut(s) 30, 486, 530, 671, 1045, 2027, 2040
Aor13HI TCCGGA 1 cut(s) 728
AoxI GGCC 5 cut(s) 11, 112, 597, 1101, 1477
ApaLI GTGCAC 2 cut(s) 53, 1798
ApeKI GCWGC 4 cut(s) 449, 475, 1066, 1978
ApoI RAATTY 9 cut(s) 750, 846, 1160, 1448, 1694, 2084, 2090, 2132, 2408
Asp700I GAANNNNTTC 4 cut(s) 609, 847, 1342, 2136
Asp718I GGTACC 1 cut(s) 1722
AspA2I CCTAGG 1 cut(s) 1642
AspLEI GCGC 3 cut(s) 89, 1414, 1654
AspS9I GGNCC 5 cut(s) 561, 1101, 1478, 1570, 1593
AsuHPI GGTGA 5 cut(s) 326, 338, 497, 955, 1880
AvaII GGWCC 3 cut(s) 561, 1570, 1593
AvrII CCTAGG 1 cut(s) 1642
BaeGI GKGCMC 3 cut(s) 57, 1802, 2185
BalI TGGCCA 1 cut(s) 13
BamHI GGATCC 1 cut(s) 2032
BanI GGYRCC 2 cut(s) 1722, 1992
BanII GRGCYC 2 cut(s) 111, 2399
BauI CACGAG 1 cut(s) 2078
BbsI GAAGAC 1 cut(s) 2061
Bbv12I GWGCWC 3 cut(s) 57, 111, 1802
BbvI GCAGC 4 cut(s) 461, 487, 1078, 1990
BccI CCATC 7 cut(s) 13, 98, 547, 1402, 1513, 2067, 2317
BciT130I CCWGG 5 cut(s) 628, 1112, 1227, 1591, 2051
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 3 cut(s) 84, 1272, 2321
BcuI ACTAGT 1 cut(s) 2317
BfaI CTAG 4 cut(s) 201, 1643, 2240, 2318
BfmI CTRYAG 3 cut(s) 404, 1064, 1510
BfoI RGCGCY 1 cut(s) 1415
BglII AGATCT 2 cut(s) 130, 2100
BisI GCNGC 8 cut(s) 85, 171, 447, 450, 476, 1067, 1498, 1979
BlnI CCTAGG 1 cut(s) 1642
BlpI GCTNAGC 3 cut(s) 453, 983, 2191
BlsI GCNGC 8 cut(s) 86, 172, 448, 451, 477, 1068, 1499, 1980
Bme1390I CCNGG 5 cut(s) 628, 1112, 1227, 1591, 2051
Bme18I GGWCC 3 cut(s) 561, 1570, 1593
BmgT120I GGNCC 5 cut(s) 561, 1101, 1478, 1570, 1593
BmiI GGNNCC 8 cut(s) 39, 562, 1102, 1572, 1588, 1724, 1994, 2034
BmrFI CCNGG 5 cut(s) 628, 1112, 1227, 1591, 2051
BmrI ACTGGG 2 cut(s) 1291, 2374
BmsI GCATC 7 cut(s) 176, 862, 895, 996, 1180, 1507, 1935
BmuI ACTGGG 2 cut(s) 1291, 2374
BpiI GAAGAC 1 cut(s) 2061
Bpu1102I GCTNAGC 3 cut(s) 453, 983, 2191
BpuEI CTTGAG 2 cut(s) 2353, 2374
Bsa29I ATCGAT 1 cut(s) 990
BsaBI GATNNNNATC 2 cut(s) 1634, 1821
BsaI GGTCTC 1 cut(s) 2321
BsaJI CCNNGG 7 cut(s) 100, 480, 495, 627, 1642, 1940, 1996
BsaWI WCCGGW 1 cut(s) 728
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 5 cut(s) 606, 686, 735, 1098, 1099
Bse1I ACTGG 5 cut(s) 1286, 1807, 2211, 2308, 2369
Bse3DI GCAATG 1 cut(s) 283
Bse8I GATNNNNATC 2 cut(s) 1634, 1821
BseAI TCCGGA 1 cut(s) 728
BseBI CCWGG 5 cut(s) 628, 1112, 1227, 1591, 2051
BseCI ATCGAT 1 cut(s) 990
BseDI CCNNGG 7 cut(s) 100, 480, 495, 627, 1642, 1940, 1996
BseGI GGATG 4 cut(s) 10, 1505, 1522, 1950
BseJI GATNNNNATC 2 cut(s) 1634, 1821
BseLI CCNNNNNNNGG 5 cut(s) 606, 686, 735, 1098, 1099
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 9 cut(s) 188, 467, 822, 974, 1751, 1838, 2012, 2200, 2449
BseNI ACTGG 5 cut(s) 1286, 1807, 2211, 2308, 2369
BseRI GAGGAG 1 cut(s) 2000
BseSI GKGCMC 3 cut(s) 57, 1802, 2185
BseXI GCAGC 4 cut(s) 461, 487, 1078, 1990
BseYI CCCAGC 2 cut(s) 239, 413
BsgI GTGCAG 1 cut(s) 494
Bsh1236I CGCG 3 cut(s) 87, 1500, 2012
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 5 cut(s) 13, 114, 599, 1103, 1479
BshNI GGYRCC 2 cut(s) 1722, 1992
BshVI ATCGAT 1 cut(s) 990
BsiEI CGRYCG 1 cut(s) 35
BsiHKAI GWGCWC 3 cut(s) 57, 111, 1802
BsiSI CCGG 1 cut(s) 729
BslFI GGGAC 3 cut(s) 1014, 1376, 1445
BslI CCNNNNNNNGG 5 cut(s) 606, 686, 735, 1098, 1099
BsmAI GTCTC 3 cut(s) 84, 1272, 2321
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 3 cut(s) 1014, 1376, 1445
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 5 cut(s) 13, 114, 599, 1103, 1479
Bso31I GGTCTC 1 cut(s) 2321
Bsp1286I GDGCHC 5 cut(s) 57, 111, 1802, 2185, 2399
Bsp13I TCCGGA 1 cut(s) 728
Bsp1407I TGTACA 1 cut(s) 2107
Bsp1720I GCTNAGC 3 cut(s) 453, 983, 2191
Bsp19I CCATGG 3 cut(s) 480, 495, 1940
BspACI CCGC 6 cut(s) 85, 140, 171, 189, 447, 1498
BspANI GGCC 5 cut(s) 13, 114, 599, 1103, 1479
BspCNI CTCAG 9 cut(s) 187, 466, 823, 975, 1750, 1839, 2013, 2199, 2448
BspDI ATCGAT 1 cut(s) 990
BspEI TCCGGA 1 cut(s) 728
BspFNI CGCG 3 cut(s) 87, 1500, 2012
BspHI TCATGA 1 cut(s) 2376
BspLI GGNNCC 8 cut(s) 39, 562, 1102, 1572, 1588, 1724, 1994, 2034
BspMAI CTGCAG 3 cut(s) 408, 1068, 1514
BspPI GGATC 7 cut(s) 30, 486, 530, 671, 1045, 2027, 2040
BspT107I GGYRCC 2 cut(s) 1722, 1992
BspTNI GGTCTC 1 cut(s) 2321
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 283
BsrGI TGTACA 1 cut(s) 2107
BsrI ACTGG 5 cut(s) 1286, 1807, 2211, 2308, 2369
BssECI CCNNGG 7 cut(s) 100, 480, 495, 627, 1642, 1940, 1996
BssSI CACGAG 1 cut(s) 2078
BssT1I CCWWGG 5 cut(s) 480, 495, 1642, 1940, 1996
Bst2BI CACGAG 1 cut(s) 2078
Bst2UI CCWGG 5 cut(s) 628, 1112, 1227, 1591, 2051
Bst4CI ACNGT 7 cut(s) 503, 785, 882, 896, 1473, 1528, 1757
Bst6I CTCTTC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 52
BstAUI TGTACA 1 cut(s) 2107
BstC8I GCNNGC 5 cut(s) 107, 1071, 1308, 1495, 2349
BstDSI CCRYGG 3 cut(s) 480, 495, 1940
BstENI CCTNNNNNAGG 2 cut(s) 604, 684
BstF5I GGATG 4 cut(s) 10, 1505, 1522, 1950
BstFNI CGCG 3 cut(s) 87, 1500, 2012
BstH2I RGCGCY 1 cut(s) 1415
BstHHI GCGC 3 cut(s) 89, 1414, 1654
BstMAI GTCTC 3 cut(s) 84, 1272, 2321
BstMCI CGRYCG 1 cut(s) 35
BstMWI GCNNNNNNNGC 6 cut(s) 52, 111, 403, 553, 776, 1931
BstNI CCWGG 5 cut(s) 628, 1112, 1227, 1591, 2051
BstNSI RCATGY 4 cut(s) 551, 1497, 1843, 2351
BstSCI CCNGG 5 cut(s) 626, 1110, 1225, 1589, 2049
BstSFI CTRYAG 3 cut(s) 404, 1064, 1510
BstSLI GKGCMC 3 cut(s) 57, 1802, 2185
BstUI CGCG 3 cut(s) 87, 1500, 2012
BstV1I GCAGC 4 cut(s) 461, 487, 1078, 1990
BstV2I GAAGAC 1 cut(s) 2061
BstX2I RGATCY 7 cut(s) 22, 130, 491, 535, 1050, 2032, 2100
BstXI CCANNNNNNTGG 1 cut(s) 1528
BstYI RGATCY 7 cut(s) 22, 130, 491, 535, 1050, 2032, 2100
Bsu15I ATCGAT 1 cut(s) 990
BsuRI GGCC 5 cut(s) 13, 114, 599, 1103, 1479
BsuTUI ATCGAT 1 cut(s) 990
BtgI CCRYGG 3 cut(s) 480, 495, 1940
BtsCI GGATG 4 cut(s) 10, 1505, 1522, 1950
BtsI GCAGTG 3 cut(s) 1061, 2154, 2446
BtsIMutI CAGTG 7 cut(s) 1061, 1533, 1800, 2154, 2218, 2362, 2446
Cac8I GCNNGC 5 cut(s) 107, 1071, 1308, 1495, 2349
CciI TCATGA 1 cut(s) 2376
CfoI GCGC 3 cut(s) 89, 1414, 1654
Cfr13I GGNCC 5 cut(s) 561, 1101, 1478, 1570, 1593
ClaI ATCGAT 1 cut(s) 990
CseI GACGC 1 cut(s) 1029
Csp6I GTAC 5 cut(s) 401, 1152, 1723, 2108, 2251
CviQI GTAC 5 cut(s) 401, 1152, 1723, 2108, 2251
DraIII CACNNNGTG 1 cut(s) 2368
EaeI YGGCCR 1 cut(s) 11
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 5 cut(s) 480, 495, 1642, 1940, 1996
Eco24I GRGCYC 2 cut(s) 111, 2399
Eco31I GGTCTC 1 cut(s) 2321
Eco47I GGWCC 3 cut(s) 561, 1570, 1593
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 4 cut(s) 665, 878, 1031, 1886
EcoICRI GAGCTC 1 cut(s) 109
EcoNI CCTNNNNNAGG 2 cut(s) 604, 684
EcoRI GAATTC 2 cut(s) 1448, 2090
EcoRII CCWGG 5 cut(s) 626, 1110, 1225, 1589, 2049
EcoT14I CCWWGG 5 cut(s) 480, 495, 1642, 1940, 1996
EcoT22I ATGCAT 2 cut(s) 1195, 1495
EcoT38I GRGCYC 2 cut(s) 111, 2399
ErhI CCWWGG 5 cut(s) 480, 495, 1642, 1940, 1996
Esp3I CGTCTC 1 cut(s) 84
FalI AAGNNNNNCTT 2 cut(s) 1884, 1916
FaqI GGGAC 3 cut(s) 1014, 1376, 1445
FauI CCCGC 1 cut(s) 182
FauNDI CATATG 1 cut(s) 1195
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 2 cut(s) 266, 863
Fnu4HI GCNGC 8 cut(s) 85, 171, 447, 450, 476, 1067, 1498, 1979
FokI GGATG 4 cut(s) 17, 1492, 1529, 1957
FriOI GRGCYC 2 cut(s) 111, 2399
Fsp4HI GCNGC 8 cut(s) 85, 171, 447, 450, 476, 1067, 1498, 1979
FspBI CTAG 4 cut(s) 201, 1643, 2240, 2318
GlaI GCGC 3 cut(s) 88, 1413, 1653
GluI GCNGC 8 cut(s) 85, 171, 447, 450, 476, 1067, 1498, 1979
GsaI CCCAGC 2 cut(s) 243, 417
HaeII RGCGCY 1 cut(s) 1415
HaeIII GGCC 5 cut(s) 13, 114, 599, 1103, 1479
HapII CCGG 1 cut(s) 729
HgaI GACGC 1 cut(s) 1029
HhaI GCGC 3 cut(s) 89, 1414, 1654
Hin6I GCGC 3 cut(s) 87, 1412, 1652
HinP1I GCGC 3 cut(s) 87, 1412, 1652
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 2 cut(s) 395, 554
HinfI GANTC 9 cut(s) 74, 148, 213, 610, 934, 1130, 1660, 1849, 1896
HpaII CCGG 1 cut(s) 729
HphI GGTGA 5 cut(s) 326, 338, 497, 955, 1880
Hpy166II GTNNAC 6 cut(s) 55, 267, 317, 864, 1800, 2110
Hpy8I GTNNAC 6 cut(s) 55, 267, 317, 864, 1800, 2110
Hpy99I CGWCG 1 cut(s) 1862
HpyAV CCTTC 9 cut(s) 198, 229, 317, 403, 688, 943, 1013, 1491, 1910
HpyCH4III ACNGT 7 cut(s) 503, 785, 882, 896, 1473, 1528, 1757
HpyCH4IV ACGT 2 cut(s) 1379, 1395
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 111, 403, 553, 776, 1931
HpySE526I ACGT 2 cut(s) 1379, 1395
HspAI GCGC 3 cut(s) 87, 1412, 1652
Kpn2I TCCGGA 1 cut(s) 728
KpnI GGTACC 1 cut(s) 1726
Ksp22I TGATCA 1 cut(s) 60
LmnI GCTCC 4 cut(s) 37, 776, 1782, 2144
Lsp1109I GCAGC 4 cut(s) 461, 487, 1078, 1990
LweI GCATC 7 cut(s) 176, 862, 895, 996, 1180, 1507, 1935
MaeI CTAG 4 cut(s) 201, 1643, 2240, 2318
MaeII ACGT 2 cut(s) 1379, 1395
MbiI CCGCTC 1 cut(s) 173
MboII GAAGA 8 cut(s) 157, 712, 929, 958, 1633, 1639, 1984, 2066
MfeI CAATTG 3 cut(s) 47, 359, 909
MflI RGATCY 7 cut(s) 22, 130, 491, 535, 1050, 2032, 2100
MhlI GDGCHC 5 cut(s) 57, 111, 1802, 2185, 2399
MlsI TGGCCA 1 cut(s) 13
MluI ACGCGT 1 cut(s) 2010
MluNI TGGCCA 1 cut(s) 13
MlyI GAGTC 3 cut(s) 68, 157, 1858
MmeI TCCRAC 1 cut(s) 15
Mox20I TGGCCA 1 cut(s) 13
Mph1103I ATGCAT 2 cut(s) 1195, 1495
MroI TCCGGA 1 cut(s) 728
MroXI GAANNNNTTC 4 cut(s) 609, 847, 1342, 2136
MscI TGGCCA 1 cut(s) 13
MseI TTAA 4 cut(s) 306, 660, 974, 1676
MslI CAYNNNNRTG 3 cut(s) 921, 992, 1526
Msp20I TGGCCA 1 cut(s) 13
MspI CCGG 1 cut(s) 729
MspR9I CCNGG 5 cut(s) 628, 1112, 1227, 1591, 2051
MunI CAATTG 3 cut(s) 47, 359, 909
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 5 cut(s) 628, 1112, 1227, 1591, 2051
MvnI CGCG 3 cut(s) 87, 1500, 2012
MwoI GCNNNNNNNGC 6 cut(s) 52, 111, 403, 553, 776, 1931
NcoI CCATGG 3 cut(s) 480, 495, 1940
NdeI CATATG 1 cut(s) 1195
NlaIV GGNNCC 8 cut(s) 39, 562, 1102, 1572, 1588, 1724, 1994, 2034
NmeAIII GCCGAG 2 cut(s) 81, 90
NmuCI GTSAC 5 cut(s) 249, 994, 1041, 1243, 1547
NsiI ATGCAT 2 cut(s) 1195, 1495
NspI RCATGY 4 cut(s) 551, 1497, 1843, 2351
OliI CACNNNNGTG 1 cut(s) 1526
PaeI GCATGC 2 cut(s) 1497, 2351
PagI TCATGA 1 cut(s) 2376
PciI ACATGT 1 cut(s) 1839
PctI GAATGC 1 cut(s) 349
PdmI GAANNNNTTC 4 cut(s) 609, 847, 1342, 2136
PfeI GAWTC 6 cut(s) 213, 610, 934, 1130, 1660, 1896
PflFI GACNNNGTC 1 cut(s) 2258
PkrI GCNGC 8 cut(s) 86, 172, 448, 451, 477, 1068, 1499, 1980
PleI GAGTC 3 cut(s) 68, 156, 1857
PpsI GAGTC 3 cut(s) 68, 156, 1857
PscI ACATGT 1 cut(s) 1839
PsiI TTATAA 2 cut(s) 375, 2294
Psp124BI GAGCTC 1 cut(s) 111
Psp6I CCWGG 5 cut(s) 626, 1110, 1225, 1589, 2049
PspFI CCCAGC 2 cut(s) 239, 413
PspGI CCWGG 5 cut(s) 626, 1110, 1225, 1589, 2049
PspN4I GGNNCC 8 cut(s) 39, 562, 1102, 1572, 1588, 1724, 1994, 2034
PspPI GGNCC 5 cut(s) 561, 1101, 1478, 1570, 1593
PstI CTGCAG 3 cut(s) 408, 1068, 1514
PsuI RGATCY 7 cut(s) 22, 130, 491, 535, 1050, 2032, 2100
PsyI GACNNNGTC 1 cut(s) 2258
RsaI GTAC 5 cut(s) 402, 1153, 1724, 2109, 2252
RsaNI GTAC 5 cut(s) 401, 1152, 1723, 2108, 2251
RseI CAYNNNNRTG 3 cut(s) 921, 992, 1526
SacI GAGCTC 1 cut(s) 111
SalI GTCGAC 1 cut(s) 265
SaqAI TTAA 4 cut(s) 306, 660, 974, 1676
SatI GCNGC 8 cut(s) 85, 171, 447, 450, 476, 1067, 1498, 1979
Sau96I GGNCC 5 cut(s) 561, 1101, 1478, 1570, 1593
SbfI CCTGCAGG 1 cut(s) 1514
SchI GAGTC 3 cut(s) 68, 157, 1858
ScrFI CCNGG 5 cut(s) 628, 1112, 1227, 1591, 2051
SdaI CCTGCAGG 1 cut(s) 1514
SduI GDGCHC 5 cut(s) 57, 111, 1802, 2185, 2399
SfaNI GCATC 7 cut(s) 176, 862, 895, 996, 1180, 1507, 1935
SfcI CTRYAG 3 cut(s) 404, 1064, 1510
SinI GGWCC 3 cut(s) 561, 1570, 1593
SmiMI CAYNNNNRTG 3 cut(s) 921, 992, 1526
SmlI CTYRAG 2 cut(s) 2332, 2353
SmoI CTYRAG 2 cut(s) 2332, 2353
SpeI ACTAGT 1 cut(s) 2317
SphI GCATGC 2 cut(s) 1497, 2351
Sse8387I CCTGCAGG 1 cut(s) 1514
SsiI CCGC 6 cut(s) 85, 140, 171, 189, 447, 1498
SspMI CTAG 4 cut(s) 201, 1643, 2240, 2318
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 5 cut(s) 626, 1110, 1225, 1589, 2049
StyI CCWWGG 5 cut(s) 480, 495, 1642, 1940, 1996
TaaI ACNGT 7 cut(s) 503, 785, 882, 896, 1473, 1528, 1757
TaiI ACGT 2 cut(s) 1382, 1398
TaqI TCGA 5 cut(s) 266, 291, 516, 990, 1857
TaqII GACCGA 1 cut(s) 21
TatI WGTACW 2 cut(s) 400, 2107
TauI GCSGC 4 cut(s) 87, 173, 449, 1500
TfiI GAWTC 6 cut(s) 213, 610, 934, 1130, 1660, 1896
Tru1I TTAA 4 cut(s) 306, 660, 974, 1676
Tru9I TTAA 4 cut(s) 306, 660, 974, 1676
TscAI CASTG 7 cut(s) 1068, 1533, 1807, 2154, 2218, 2369, 2446
TseFI GTSAC 5 cut(s) 249, 994, 1041, 1243, 1547
TseI GCWGC 4 cut(s) 449, 475, 1066, 1978
Tsp45I GTSAC 5 cut(s) 249, 994, 1041, 1243, 1547
TspGWI ACGGA 3 cut(s) 141, 553, 1139
TspRI CASTG 7 cut(s) 1068, 1533, 1807, 2154, 2218, 2369, 2446
Tth111I GACNNNGTC 1 cut(s) 2258
VneI GTGCAC 2 cut(s) 53, 1798
VpaK11BI GGWCC 3 cut(s) 561, 1570, 1593
XagI CCTNNNNNAGG 2 cut(s) 604, 684
XapI RAATTY 9 cut(s) 750, 846, 1160, 1448, 1694, 2084, 2090, 2132, 2408
XceI RCATGY 4 cut(s) 551, 1497, 1843, 2351
XmaJI CCTAGG 1 cut(s) 1642
XmiI GTMKAC 2 cut(s) 266, 863
XmnI GAANNNNTTC 4 cut(s) 609, 847, 1342, 2136
XspI CTAG 4 cut(s) 201, 1643, 2240, 2318
Zsp2I ATGCAT 2 cut(s) 1195, 1495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.