RchiOBHm_Chr6g0302051

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003618
Physical Location & Seq
Forward (+)
62247570 .. 62253525
5956 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27132

Sequence Viewer

Length: 2466 bp
ATGGTGACGGAGGATTGTTGGCCAACAATGGATCTACTGCGGTCGGAGCCAATGCAATTGGTGCACCTCATCATTCCGATCGAATCGTCTCGCCGCGCCATCTCCTACCTCGGCGACCTTGGCCTTTTTCAATTCAAAGATCTCAATGCGGAGAAGAGTCCTTTCCAGAGAACATATGCCGCTCAGATCAAACGATGTGGGGAAATGGCTCGAAGGTTACGTTTCTTCAGAGATCAGATGAACAAGGCTGGTTTGTCACATTCAACAATGTCTACAATGAGCGATGATGATAATGATCTTGATCGCTTGGAGGTTAAACTCACTGAACTTGAAGCTGATCTATTAGAAATGAATTCAAACAATGAACAATTACAACGCACTTATAGTGAGCTATTAGAATACAAGCTTGTACTGCAGAAGGCTGGCGGGTTTTTTTGTTCAGCTCAAAGTAGTGCCACAGCTCAGCAAAGAGAAATTGAAGTGCAGCCCCTGGGTGAAAGATCCATTGACAGTCCATTATTACTCGAACAAGAAATGACAACAGATCCATCGAAGCATGTAAAGCTTGGGTCCGTGAGTGGTCTTGTTCCAAGAGAAAAGTCTTTGGCATTCGAAAGGATTTTATTTCGTGCCACCAGGGGTAATGTATTTCTGAAGCAAGCTGTGGTCAATGGCCCTGTTGTTGATCCTGTGTCAGGAGAGAAGGTTGAGAAAAACGTATTTATCATCTTCTATTCTGGCGAAAGGGCAAAGAATAAAATTCTGAAAATATGTGAGGCTTTTAGAGCAAACCGTTACCCATTTACAGATGACTTGGGAAAACAATTTCAGATGATTACAGAGGTGTCTGCAAAACTTTCAGAGCTGAAGTCTACCATAGATGCTGGACTATTGCACCGGAACAGTTTATTACAGACAATTGGCCATCAATATGAGCAATGGAACCTTCTGGTAAAGAAGGAAAAATCCATCTACCACACCTTAAACATGCTCAGCATTGATGTGACAAAGATGTGTCTTGTTGCAGAAGGTTGGTGTCCTGTATCCGCATCAATTCAGATCCAAAACGCATTGCAGCAGGCAACCTTTGATACCAACTGTCAAGTTGGGGCCATATTCCAGGTTTTGCAGACAAAGGAATCGCCACCAACATATTTCCGTACAAACAAATTTACTTCTGCTTTTCAAGAAATTGTTGACGCATATGGGGTTGCCAAGTATCAGGAGGCTAATCCTGGTGTATACACCATTGTCACTTTCCCTTTCCTTTTCGCTGTGATGTTTGGAGATTGGGGGCATGGTATTTGCTTGTTGCTGGCAACACTATATTTCATCATCAGGGAGAGGAAGTTTTCTAGTCAGAAACTTGGGGACATTATTGAAATGACGTTTGGTGGCCGTTATGTTATTTTTATGATGGCGCTGTTCTCAATTTACACAGGATTGATATATAATGAATTCTTTTCAGTCCCATTTGAAATATTTGGGCCTTCTGCCTATGGGTGCCGTGACCCATCCTGCAGGGATGCCACTACAGCAGGTTTGACTAAGGTGCGTGACACCTATCCATTTGGTGTGGATCCTAAATGGCACGGTTCCAGGTCTGAATTACCCTTTCTTAACTCGTTGAAAATGAAGATGTCAATCCTACTTGGAGTAGCCCAGATGAATCTTGGAATTGTATTAAGTTACTTCAATGCAAAATATTTTGCGGATAAGCTGAATATCTGGTACCAATTTGTTCCTCAGATGATATTTCTAAACAGTCTGTTTGGCTACCTTTCACTCCTCATTATTGTGAAATGGTGCACTGGTTCAAAAGCTGATTTGTATCATGTAATGATATACATGTTCCTGAGTCCTTTCGAAGATCTGGGTGAAAATCAGCTCTTTTGGGGGCAAGGATTCCTTCAGGTTCTATTATTACTATCCGCTCTTGTTGCTGTGCCATGGATGCTGTTTCCGAAGCCTTATTTCTTGAAAAAGCAGCATGAGGAGAGGCACCAAGGTCAATCATACGCGTTGCTTCTGACTGGGGAGGACCCTCTTGAAGATGACCAGGAAGACAACCACAATTCAAAAGGTCATGAGGAATTTGAATTCAGCGAGATCTTTGTACACCAACTCATACATACCATAGAGTTTGTGCTTGGAGCAGTGTCCAATACAGCTTCATATCTACGTTTATGGGCACTCAGCTTAGCACATTCAGAGTTGTCCAGTGTATTTTACGACAAGGTTCTGCTTCTAGCATGGGGGTTCAACAATGTCATTATTCTAATTATTGGCATAATCGTTTTTATAAGTGCAACTGTTGGAGTGCTACTAGTGATGGAGACCTTAAGTGCATTCTTGCATGCCTTGAGGCTTCACTGGGTGGAGTTTCAGAACAAGTTTTACGAGGGCGCTGGTTACAAATTCTGCCCGTTTTCATTTGCATTACTCAGTAGTAAGGATGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

821

Amino Acids

93.55

Weight (kDa)

5.84

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 36 - 812 9.3e-304 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2303
Acc36I ACCTGC 1 cut(s) 1529
Acc65I GGTACC 1 cut(s) 1731
AccB1I GGYRCC 3 cut(s) 1503, 1731, 2001
AccBSI CCGCTC 2 cut(s) 182, 1934
AccI GTMKAC 3 cut(s) 272, 872, 1242
AccII CGCG 2 cut(s) 96, 2021
AciI CCGC 8 cut(s) 40, 94, 149, 180, 426, 1047, 1712, 1932
AclWI GGATC 7 cut(s) 39, 495, 539, 680, 1054, 1574, 1587
AcoI YGGCCR 3 cut(s) 20, 922, 1396
AcsI RAATTY 7 cut(s) 352, 759, 1169, 1457, 2093, 2099, 2417
AcuI CTGAAG 4 cut(s) 211, 674, 887, 1895
AdeI CACNNNGTG 1 cut(s) 2377
AfaI GTAC 4 cut(s) 411, 1162, 1733, 2118
AfiI CCNNNNNNNGG 1 cut(s) 695
AflII CTTAAG 1 cut(s) 2341
AflIII ACRYGT 2 cut(s) 1848, 2019
AhlI ACTAGT 1 cut(s) 2326
AjnI CCWGG 6 cut(s) 489, 635, 1119, 1234, 1598, 2058
AjuI GAANNNNNNNTTGG 2 cut(s) 1374, 1406
Alw21I GWGCWC 2 cut(s) 66, 1811
Alw26I GTCTC 2 cut(s) 93, 2330
Alw44I GTGCAC 2 cut(s) 62, 1807
AlwI GGATC 7 cut(s) 39, 495, 539, 680, 1054, 1574, 1587
AlwNI CAGNNNCTG 1 cut(s) 490
AoxI GGCC 7 cut(s) 20, 121, 673, 922, 1110, 1396, 1487
ApaLI GTGCAC 2 cut(s) 62, 1807
ApeKI GCWGC 3 cut(s) 484, 1075, 1987
ApoI RAATTY 7 cut(s) 352, 759, 1169, 1457, 2093, 2099, 2417
Asp718I GGTACC 1 cut(s) 1731
AspLEI GCGC 3 cut(s) 98, 1423, 2408
AspS9I GGNCC 5 cut(s) 570, 674, 1110, 1487, 2041
AsuHPI GGTGA 3 cut(s) 16, 506, 1889
AsuII TTCGAA 2 cut(s) 612, 1866
AvaII GGWCC 2 cut(s) 570, 2041
BaeGI GKGCMC 3 cut(s) 66, 1811, 2194
BalI TGGCCA 2 cut(s) 22, 924
BamHI GGATCC 1 cut(s) 1579
BanI GGYRCC 3 cut(s) 1503, 1731, 2001
BbsI GAAGAC 1 cut(s) 2070
Bbv12I GWGCWC 2 cut(s) 66, 1811
BbvI GCAGC 3 cut(s) 496, 1087, 1999
BccI CCATC 7 cut(s) 107, 556, 933, 977, 1411, 1522, 2326
BceAI ACGGC 2 cut(s) 1383, 1491
BciT130I CCWGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
BciVI GTATCC 1 cut(s) 1054
BcoDI GTCTC 2 cut(s) 93, 2330
BcuI ACTAGT 1 cut(s) 2326
BfaI CTAG 3 cut(s) 1356, 2249, 2327
BfmI CTRYAG 3 cut(s) 413, 1519, 1533
BfoI RGCGCY 2 cut(s) 1424, 2409
BfrI CTTAAG 1 cut(s) 2341
BfuAI ACCTGC 1 cut(s) 1529
BfuI GTATCC 1 cut(s) 1054
BglII AGATCT 3 cut(s) 139, 1870, 2109
BisI GCNGC 5 cut(s) 94, 180, 485, 1076, 1988
BlpI GCTNAGC 3 cut(s) 462, 992, 2200
BlsI GCNGC 5 cut(s) 95, 181, 486, 1077, 1989
Bme1390I CCNGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
Bme18I GGWCC 2 cut(s) 570, 2041
BmgT120I GGNCC 5 cut(s) 570, 674, 1110, 1487, 2041
BmrFI CCNGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
BmrI ACTGGG 2 cut(s) 2043, 2383
BmsI GCATC 4 cut(s) 871, 1058, 1516, 1944
BmuI ACTGGG 2 cut(s) 2043, 2383
BpiI GAAGAC 1 cut(s) 2070
Bpu1102I GCTNAGC 3 cut(s) 462, 992, 2200
Bpu14I TTCGAA 2 cut(s) 612, 1866
BpuEI CTTGAG 1 cut(s) 2383
BsaBI GATNNNNATC 4 cut(s) 294, 300, 1643, 1830
BsaI GGTCTC 1 cut(s) 2330
BsaJI CCNNGG 7 cut(s) 109, 118, 489, 490, 636, 1949, 2005
BsaWI WCCGGW 1 cut(s) 897
BsaXI ACNNNNNCTCC 4 cut(s) 690, 720, 1334, 1364
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse1I ACTGG 4 cut(s) 1816, 2038, 2220, 2378
Bse3DI GCAATG 2 cut(s) 944, 1070
Bse8I GATNNNNATC 4 cut(s) 294, 300, 1643, 1830
BseBI CCWGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
BseDI CCNNGG 7 cut(s) 109, 118, 489, 490, 636, 1949, 2005
BseGI GGATG 4 cut(s) 1514, 1531, 1959, 2461
BseJI GATNNNNATC 4 cut(s) 294, 300, 1643, 1830
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMI GCAATG 2 cut(s) 944, 1070
BseMII CTCAG 7 cut(s) 197, 476, 1006, 1760, 1847, 2209, 2458
BseNI ACTGG 4 cut(s) 1816, 2038, 2220, 2378
BseRI GAGGAG 2 cut(s) 1778, 2009
BseSI GKGCMC 3 cut(s) 66, 1811, 2194
BseXI GCAGC 3 cut(s) 496, 1087, 1999
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 2 cut(s) 96, 2021
Bsh1285I CGRYCG 2 cut(s) 44, 81
BshFI GGCC 7 cut(s) 22, 123, 675, 924, 1112, 1398, 1489
BshNI GGYRCC 3 cut(s) 1503, 1731, 2001
BsiEI CGRYCG 2 cut(s) 44, 81
BsiHKAI GWGCWC 2 cut(s) 66, 1811
BsiSI CCGG 1 cut(s) 898
BslFI GGGAC 2 cut(s) 1385, 1454
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 2 cut(s) 93, 2330
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 1385, 1454
BsmI GAATGC 2 cut(s) 608, 2348
BsnI GGCC 7 cut(s) 22, 123, 675, 924, 1112, 1398, 1489
Bso31I GGTCTC 1 cut(s) 2330
Bsp119I TTCGAA 2 cut(s) 612, 1866
Bsp1286I GDGCHC 3 cut(s) 66, 1811, 2194
Bsp1407I TGTACA 1 cut(s) 2116
Bsp1720I GCTNAGC 3 cut(s) 462, 992, 2200
Bsp19I CCATGG 1 cut(s) 1949
BspACI CCGC 8 cut(s) 40, 94, 149, 180, 426, 1047, 1712, 1932
BspANI GGCC 7 cut(s) 22, 123, 675, 924, 1112, 1398, 1489
BspCNI CTCAG 7 cut(s) 196, 475, 1005, 1759, 1848, 2208, 2457
BspFNI CGCG 2 cut(s) 96, 2021
BspHI TCATGA 1 cut(s) 2086
BspMAI CTGCAG 2 cut(s) 417, 1523
BspMI ACCTGC 1 cut(s) 1529
BspPI GGATC 7 cut(s) 39, 495, 539, 680, 1054, 1574, 1587
BspT104I TTCGAA 2 cut(s) 612, 1866
BspT107I GGYRCC 3 cut(s) 1503, 1731, 2001
BspTI CTTAAG 1 cut(s) 2341
BspTNI GGTCTC 1 cut(s) 2330
BsrBI CCGCTC 2 cut(s) 182, 1934
BsrDI GCAATG 2 cut(s) 944, 1070
BsrGI TGTACA 1 cut(s) 2116
BsrI ACTGG 4 cut(s) 1816, 2038, 2220, 2378
BssECI CCNNGG 7 cut(s) 109, 118, 489, 490, 636, 1949, 2005
BssNAI GTATAC 1 cut(s) 1243
BssT1I CCWWGG 3 cut(s) 118, 1949, 2005
Bst1107I GTATAC 1 cut(s) 1243
Bst2UI CCWGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
Bst4CI ACNGT 7 cut(s) 512, 794, 905, 1100, 1595, 1766, 2314
Bst6I CTCTTC 1 cut(s) 149
BstAFI CTTAAG 1 cut(s) 2341
BstAPI GCANNNNNTGC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 2116
BstBI TTCGAA 2 cut(s) 612, 1866
BstC8I GCNNGC 5 cut(s) 424, 660, 1080, 1317, 2358
BstDEI CTNAG 9 cut(s) 183, 462, 992, 1548, 1746, 1856, 2195, 2200, 2444
BstDSI CCRYGG 1 cut(s) 1949
BstENI CCTNNNNNAGG 1 cut(s) 693
BstF5I GGATG 4 cut(s) 1514, 1531, 1959, 2461
BstFNI CGCG 2 cut(s) 96, 2021
BstH2I RGCGCY 2 cut(s) 1424, 2409
BstHHI GCGC 3 cut(s) 98, 1423, 2408
BstMAI GTCTC 2 cut(s) 93, 2330
BstMCI CGRYCG 2 cut(s) 44, 81
BstMWI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 412, 562, 785, 1535, 1940, 1954
BstNI CCWGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
BstNSI RCATGY 4 cut(s) 560, 991, 1852, 2360
BstSCI CCNGG 6 cut(s) 489, 635, 1119, 1234, 1598, 2058
BstSFI CTRYAG 3 cut(s) 413, 1519, 1533
BstSLI GKGCMC 3 cut(s) 66, 1811, 2194
BstUI CGCG 2 cut(s) 96, 2021
BstV1I GCAGC 3 cut(s) 496, 1087, 1999
BstV2I GAAGAC 1 cut(s) 2070
BstX2I RGATCY 8 cut(s) 31, 139, 500, 544, 1059, 1579, 1870, 2109
BstYI RGATCY 8 cut(s) 31, 139, 500, 544, 1059, 1579, 1870, 2109
BstZ17I GTATAC 1 cut(s) 1243
BsuI GTATCC 1 cut(s) 1054
BsuRI GGCC 7 cut(s) 22, 123, 675, 924, 1112, 1398, 1489
BtgI CCRYGG 1 cut(s) 1949
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 4 cut(s) 1514, 1531, 1959, 2461
BtsI GCAGTG 1 cut(s) 2163
BtsIMutI CAGTG 5 cut(s) 321, 1809, 2163, 2227, 2371
BveI ACCTGC 1 cut(s) 1529
Cac8I GCNNGC 5 cut(s) 424, 660, 1080, 1317, 2358
CaiI CAGNNNCTG 1 cut(s) 490
CciI TCATGA 1 cut(s) 2086
CfoI GCGC 3 cut(s) 98, 1423, 2408
Cfr13I GGNCC 5 cut(s) 570, 674, 1110, 1487, 2041
CseI GACGC 1 cut(s) 1208
Csp6I GTAC 4 cut(s) 410, 1161, 1732, 2117
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviQI GTAC 4 cut(s) 410, 1161, 1732, 2117
DdeI CTNAG 9 cut(s) 183, 462, 992, 1548, 1746, 1856, 2195, 2200, 2444
DraIII CACNNNGTG 1 cut(s) 2377
EaeI YGGCCR 3 cut(s) 20, 922, 1396
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco130I CCWWGG 3 cut(s) 118, 1949, 2005
Eco31I GGTCTC 1 cut(s) 2330
Eco47I GGWCC 2 cut(s) 570, 2041
Eco57I CTGAAG 4 cut(s) 211, 674, 887, 1895
EcoNI CCTNNNNNAGG 1 cut(s) 693
EcoO109I RGGNCCY 1 cut(s) 2041
EcoRI GAATTC 3 cut(s) 352, 1457, 2099
EcoRII CCWGG 6 cut(s) 489, 635, 1119, 1234, 1598, 2058
EcoT14I CCWWGG 3 cut(s) 118, 1949, 2005
ErhI CCWWGG 3 cut(s) 118, 1949, 2005
Esp3I CGTCTC 1 cut(s) 93
FalI AAGNNNNNCTT 2 cut(s) 1893, 1925
FaqI GGGAC 2 cut(s) 1385, 1454
FauI CCCGC 1 cut(s) 419
FauNDI CATATG 2 cut(s) 175, 1204
FblI GTMKAC 3 cut(s) 272, 872, 1242
Fnu4HI GCNGC 5 cut(s) 94, 180, 485, 1076, 1988
FokI GGATG 3 cut(s) 1501, 1538, 1966
Fsp4HI GCNGC 5 cut(s) 94, 180, 485, 1076, 1988
FspBI CTAG 3 cut(s) 1356, 2249, 2327
GlaI GCGC 3 cut(s) 97, 1422, 2407
GluI GCNGC 5 cut(s) 94, 180, 485, 1076, 1988
HaeII RGCGCY 2 cut(s) 1424, 2409
HaeIII GGCC 7 cut(s) 22, 123, 675, 924, 1112, 1398, 1489
HapII CCGG 1 cut(s) 898
HgaI GACGC 1 cut(s) 1208
HhaI GCGC 3 cut(s) 98, 1423, 2408
Hin6I GCGC 3 cut(s) 96, 1421, 2406
HinP1I GCGC 3 cut(s) 96, 1421, 2406
HincII GTYRAC 1 cut(s) 1198
HindII GTYRAC 1 cut(s) 1198
HindIII AAGCTT 2 cut(s) 404, 563
HinfI GANTC 6 cut(s) 83, 157, 1139, 1669, 1858, 1905
HpaII CCGG 1 cut(s) 898
HphI GGTGA 3 cut(s) 16, 506, 1889
Hpy166II GTNNAC 7 cut(s) 64, 273, 873, 1198, 1243, 1809, 2119
Hpy188III TCNNGA 9 cut(s) 166, 299, 696, 1187, 1223, 1855, 1978, 2048, 2087
Hpy8I GTNNAC 7 cut(s) 64, 273, 873, 1198, 1243, 1809, 2119
HpyAV CCTTC 8 cut(s) 207, 412, 697, 952, 956, 1022, 1500, 1919
HpyCH4III ACNGT 7 cut(s) 512, 794, 905, 1100, 1595, 1766, 2314
HpyCH4IV ACGT 4 cut(s) 220, 717, 1388, 2182
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 412, 562, 785, 1535, 1940, 1954
HpyF3I CTNAG 9 cut(s) 183, 462, 992, 1548, 1746, 1856, 2195, 2200, 2444
HpySE526I ACGT 4 cut(s) 220, 717, 1388, 2182
HspAI GCGC 3 cut(s) 96, 1421, 2406
KpnI GGTACC 1 cut(s) 1735
LmnI GCTCC 2 cut(s) 46, 2153
Lsp1109I GCAGC 3 cut(s) 496, 1087, 1999
LweI GCATC 4 cut(s) 871, 1058, 1516, 1944
MaeI CTAG 3 cut(s) 1356, 2249, 2327
MaeII ACGT 4 cut(s) 220, 717, 1388, 2182
MbiI CCGCTC 2 cut(s) 182, 1934
MboII GAAGA 7 cut(s) 166, 217, 721, 1648, 1880, 2063, 2075
MfeI CAATTG 2 cut(s) 56, 918
MflI RGATCY 8 cut(s) 31, 139, 500, 544, 1059, 1579, 1870, 2109
MhlI GDGCHC 3 cut(s) 66, 1811, 2194
MlsI TGGCCA 2 cut(s) 22, 924
MluI ACGCGT 1 cut(s) 2019
MluNI TGGCCA 2 cut(s) 22, 924
MlyI GAGTC 2 cut(s) 166, 1867
MmeI TCCRAC 2 cut(s) 24, 2296
Mox20I TGGCCA 2 cut(s) 22, 924
MscI TGGCCA 2 cut(s) 22, 924
MseI TTAA 5 cut(s) 315, 983, 1620, 1685, 2342
MslI CAYNNNNRTG 3 cut(s) 930, 1001, 1796
Msp20I TGGCCA 2 cut(s) 22, 924
MspCI CTTAAG 1 cut(s) 2341
MspI CCGG 1 cut(s) 898
MspR9I CCNGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
MunI CAATTG 2 cut(s) 56, 918
Mva1269I GAATGC 2 cut(s) 608, 2348
MvaI CCWGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
MvnI CGCG 2 cut(s) 96, 2021
MwoI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 412, 562, 785, 1535, 1940, 1954
NcoI CCATGG 1 cut(s) 1949
NdeI CATATG 2 cut(s) 175, 1204
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 6 cut(s) 4, 255, 1003, 1252, 1508, 1556
NspI RCATGY 4 cut(s) 560, 991, 1852, 2360
NspV TTCGAA 2 cut(s) 612, 1866
PaeI GCATGC 1 cut(s) 2360
PagI TCATGA 1 cut(s) 2086
PasI CCCWGGG 1 cut(s) 490
PciI ACATGT 1 cut(s) 1848
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PctI GAATGC 2 cut(s) 608, 2348
PfeI GAWTC 4 cut(s) 83, 1139, 1669, 1905
PkrI GCNGC 5 cut(s) 95, 181, 486, 1077, 1989
Ple19I CGATCG 1 cut(s) 81
PleI GAGTC 2 cut(s) 165, 1866
PpsI GAGTC 2 cut(s) 165, 1866
PpuMI RGGWCCY 1 cut(s) 2041
PscI ACATGT 1 cut(s) 1848
PsiI TTATAA 1 cut(s) 2303
Psp5II RGGWCCY 1 cut(s) 2041
Psp6I CCWGG 6 cut(s) 489, 635, 1119, 1234, 1598, 2058
PspGI CCWGG 6 cut(s) 489, 635, 1119, 1234, 1598, 2058
PspPI GGNCC 5 cut(s) 570, 674, 1110, 1487, 2041
PspPPI RGGWCCY 1 cut(s) 2041
PsrI GAACNNNNNNTAC 2 cut(s) 2381, 2413
PstI CTGCAG 2 cut(s) 417, 1523
PstNI CAGNNNCTG 1 cut(s) 490
PsuI RGATCY 8 cut(s) 31, 139, 500, 544, 1059, 1579, 1870, 2109
PvuI CGATCG 1 cut(s) 81
RsaI GTAC 4 cut(s) 411, 1162, 1733, 2118
RsaNI GTAC 4 cut(s) 410, 1161, 1732, 2117
RseI CAYNNNNRTG 3 cut(s) 930, 1001, 1796
SaqAI TTAA 5 cut(s) 315, 983, 1620, 1685, 2342
SatI GCNGC 5 cut(s) 94, 180, 485, 1076, 1988
Sau96I GGNCC 5 cut(s) 570, 674, 1110, 1487, 2041
SbfI CCTGCAGG 1 cut(s) 1523
SchI GAGTC 2 cut(s) 166, 1867
ScrFI CCNGG 6 cut(s) 491, 637, 1121, 1236, 1600, 2060
SdaI CCTGCAGG 1 cut(s) 1523
SduI GDGCHC 3 cut(s) 66, 1811, 2194
SfaNI GCATC 4 cut(s) 871, 1058, 1516, 1944
SfcI CTRYAG 3 cut(s) 413, 1519, 1533
SfuI TTCGAA 2 cut(s) 612, 1866
SinI GGWCC 2 cut(s) 570, 2041
SmiMI CAYNNNNRTG 3 cut(s) 930, 1001, 1796
SmlI CTYRAG 2 cut(s) 2341, 2362
SmoI CTYRAG 2 cut(s) 2341, 2362
SpeI ACTAGT 1 cut(s) 2326
SphI GCATGC 1 cut(s) 2360
Sse8387I CCTGCAGG 1 cut(s) 1523
SsiI CCGC 8 cut(s) 40, 94, 149, 180, 426, 1047, 1712, 1932
SspI AATATT 2 cut(s) 1482, 1706
SspMI CTAG 3 cut(s) 1356, 2249, 2327
StyD4I CCNGG 6 cut(s) 489, 635, 1119, 1234, 1598, 2058
StyI CCWWGG 3 cut(s) 118, 1949, 2005
TaaI ACNGT 7 cut(s) 512, 794, 905, 1100, 1595, 1766, 2314
TaiI ACGT 4 cut(s) 223, 720, 1391, 2185
TaqI TCGA 6 cut(s) 81, 211, 525, 551, 612, 1866
TatI WGTACW 2 cut(s) 409, 2116
TauI GCSGC 2 cut(s) 96, 182
TfiI GAWTC 4 cut(s) 83, 1139, 1669, 1905
Tru1I TTAA 5 cut(s) 315, 983, 1620, 1685, 2342
Tru9I TTAA 5 cut(s) 315, 983, 1620, 1685, 2342
TscAI CASTG 5 cut(s) 328, 1816, 2163, 2227, 2378
TseFI GTSAC 6 cut(s) 4, 255, 1003, 1252, 1508, 1556
TseI GCWGC 3 cut(s) 484, 1075, 1987
Tsp45I GTSAC 6 cut(s) 4, 255, 1003, 1252, 1508, 1556
TspDTI ATGAA 9 cut(s) 254, 365, 378, 1321, 1470, 1649, 1682, 2163, 2421
TspGWI ACGGA 3 cut(s) 23, 562, 1148
TspRI CASTG 5 cut(s) 328, 1816, 2163, 2227, 2378
Vha464I CTTAAG 1 cut(s) 2341
VneI GTGCAC 2 cut(s) 62, 1807
VpaK11BI GGWCC 2 cut(s) 570, 2041
XagI CCTNNNNNAGG 1 cut(s) 693
XapI RAATTY 7 cut(s) 352, 759, 1169, 1457, 2093, 2099, 2417
XceI RCATGY 4 cut(s) 560, 991, 1852, 2360
XcmI CCANNNNNNNNNTGG 1 cut(s) 1670
XmiI GTMKAC 3 cut(s) 272, 872, 1242
XspI CTAG 3 cut(s) 1356, 2249, 2327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.