Prupe.8G088600_v2.0.a1

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0003618
Physical Location & Seq
Forward (+)
12176566 .. 12187914
11349 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G088600.1

Sequence Viewer

Length: 2445 bp
ATGGGAGAGTGCTGTCCTCCGATGGATCTGTTCAGGTCGGAGCCTATGCAGCTCGTCCAGATCATCATCCCCATCGAGTCTGCTCACCTCACTGTCTCTTATCTCGGTGACCTCGGCCTCCTCCAGTTCAAAGACCTCAATGCAGAGAAGAGCCCGTTTCAGAGGACTTATGCTGCCCAGATTAAAAGATCTGCAGAGATGGCTCGCAAATTACGTTTCTTCAAGGACCAAATGTTAAAGGCAAATTTGCCTTCTTCAAAGTCTAAAAGACAAGTTGACGTCAATGTGGACAACCTAGAGGTAAAACTTGGTGAATTTGAGGCCGAGCTGATTGAAATAAATTCGAACAGTGAAAAGTTGCAGCGTTCATATAATGAACTGATAGAATATAAGCTTGTTCTGGAAAAGGCTGGTGAGTTTTTTCACTCAGCTCAAAGCAGTGCCGCGTTGCAGCAAAGAGAAAATGAATCCCGTCATATTGGTGATGAATCTCTGGACACGCCTTTATTACTGGAGCAAGAAGCATCAACTGATCCATCAAAGCAAGTTAAGTTGGGGTTTCTCACTGGACTTGTTCCTAGGGGAAAGTCTTTGGCATTTGAGAGGATTCTTTTTCGAGCTACTAGGGGTAATGTCTTTCTGAGGCAGGCTGTGGTTGAAAATCCTGTCACCGATCCTGTTTCAGGAGAGAAGGTTGAGAAAAATGTTTTTGTGGTATTCTACTCTGGAGAAAGAGCAAAGAACAAAATTCTTAAAATATGTGAAGCTTTTGGAGCCAATCGTTACTCTTTTCCTGAGGATCTGGGTAGGCAAGCTCAAATGATTACTGAGGTTTCAGGAAGAATTTCAGAGCTAAAGACTACTATAGATATCGGACTGTTGCATCAGGGAAGTTTGTTACAGAATATTGGGGAGCATTTTGAGCATTGGAACCTTTTGGTGAGGAAGGAGAAATCCATATACCATCATCTTAACATGCTTAGCCTTGATGTAACAAAAAAGTGTCTTGTGGCTGAGGGGTGGAGTCCTATTTTTGCATCAAAACAGATCCAGGATGCATTGCAGCGGGCAGCATTTGACTCCAACTCACAAGTTGGCGCTATTTTCCAGGTTTTGCACACACAGGAGGCACCACCTACCTATTTCCGCACAAACAAATTCACTTCTTCTTTTCAAGAAATTGTTGAGGCATATGGGGTAGCTAAGTATCAAGAAGCAAATCCTGCTGTATATACTATCGTGACATTCCCATTTCTTTTTGCTGTCATGTTTGGTGATTGGGGACATGGAATATGTTTGTTACTTGCGACACTATATTTAATAGGCAGGGAAAGGAAACTTTCTAGTCAGAAGCTTGGAGACATCATGGAGATGGCCTTTGGTGGCCGCTATGTTATTTTGTTGATGGCAATTTTCTCAATCTACACAGGTTTAATCTATAATGAGTTCTTCTCTGTCCCATTTGAACTGTTTGGCAGCTCAGCATATGCGTGCCGTGATCTTTCTTGCAGAGACGCTACTACAGCTGGTTTGATTAAGGTGCGTCCCACTTACCCATTTGGCCTGGATCCTGTATGGCATGGATCCCGCAGTGAGCTTCCATTTCTAAACTCATTGAAGATGAAAATGTCAATTCTTCTTGGGGTGGTCCAAATGAACCTTGGAATTATATTAAGTTTTTTCAACGCCAGGTTCTTTCGAAGTGGAGTTAACGTTTGGTTCCAGTTTGTACCCCAGATTATATTTTTAAACAGTCTATTTGGCTACCTGTCCGTTCTCATTGTTATGAAGTGGTGGACTGGTTCAAAAGCTGATCTATACCATGTAATGATATACATGTTCCTAAGTCCTACAGACGAGCTAGGTGAAAACCAGCTTTTCAGTGGTCAGAGAACAGTTCAGCTTGTGCTATTACTATTGGCTTTTGTTTCTGTACCATGGATGCTGTTTCCAAAGCCCTTTATATTGAAGAAGCAACATCAAGATCGGCACCAAGGCCAATCCTATGCCCTACTTGAGAACACAGAAGAGAGCTTGCAAGTGAATTCAAACCATGATGCCCATGGTCATGGCGAGTTTGAATTTAGTGAAGTTTTCGTACATCAAATGATACATACAATAGAATTTGTACTTGGAGCAGTCTCAAATACAGCTTCATACCTTCGTCTATGGGCTCTAAGTCTTGCTCATTCAGAGTTGTCCAGTGTGTTCTATGACAAGGTTCTCCTGCTGGCTTGGGGGTTCAACAACGTGATCATACTCATCGTGGGTATTATCGTCTTCATTTGCGCAACTGTCGGTGTTTTACTGCTGATGGAAACTCTCAGTGCTTTCCTTCATGCTTTGCGACTTCACTGGGTGGAATTCCAGAACAAGTTTTACGAGGGAGATGGTTACAAGTTCTATCCGTTCTCATTCGCATTGCTTGACGACGAAGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

815

Amino Acids

92.52

Weight (kDa)

6.09

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
Acc16I TGCGCA 1 cut(s) 2290
AccB1I GGYRCC 2 cut(s) 1129, 1989
AccII CGCG 1 cut(s) 446
AciI CCGC 5 cut(s) 444, 1066, 1147, 1387, 1588
AclI AACGTT 1 cut(s) 1713
AclWI GGATC 9 cut(s) 33, 527, 668, 807, 1042, 1562, 1575, 1578, 1591
AcoI YGGCCR 1 cut(s) 1384
AcyI GRCGYC 1 cut(s) 279
AdeI CACNNNGTG 1 cut(s) 2359
AfaI GTAC 4 cut(s) 1731, 1935, 2100, 2130
AfiI CCNNNNNNNGG 1 cut(s) 683
AflIII ACRYGT 1 cut(s) 1836
AjnI CCWGG 4 cut(s) 1050, 1107, 1563, 1688
AjuI GAANNNNNNNTTGG 2 cut(s) 2115, 2147
Alw26I GTCTC 4 cut(s) 100, 1353, 1506, 2146
AlwI GGATC 9 cut(s) 33, 527, 668, 807, 1042, 1562, 1575, 1578, 1591
AoxI GGCC 6 cut(s) 115, 321, 1374, 1384, 1561, 1996
ApeKI GCWGC 7 cut(s) 49, 173, 361, 451, 1063, 1070, 1476
Asp700I GAANNNNTTC 1 cut(s) 844
AspA2I CCTAGG 1 cut(s) 578
AspLEI GCGC 2 cut(s) 1100, 2291
AspS9I GGNCC 2 cut(s) 226, 1648
AsuHPI GGTGA 9 cut(s) 77, 119, 323, 425, 494, 661, 952, 1286, 1877
AsuII TTCGAA 2 cut(s) 344, 1699
AvaII GGWCC 2 cut(s) 226, 1648
AvrII CCTAGG 1 cut(s) 578
AxyI CCTNAGG 1 cut(s) 795
BamHI GGATCC 2 cut(s) 1567, 1583
BanI GGYRCC 2 cut(s) 1129, 1989
BanII GRGCYC 2 cut(s) 155, 2176
BarI GAAGNNNNNNTAC 2 cut(s) 2082, 2114
BbsI GAAGAC 1 cut(s) 2272
BbvCI CCTCAGC 1 cut(s) 1014
BbvI GCAGC 7 cut(s) 61, 160, 373, 463, 1075, 1082, 1488
BccI CCATC 9 cut(s) 16, 80, 193, 544, 972, 1366, 1399, 2308, 2384
BceAI ACGGC 1 cut(s) 1479
BciT130I CCWGG 4 cut(s) 1052, 1109, 1565, 1690
BclI TGATCA 1 cut(s) 2253
BcoDI GTCTC 4 cut(s) 100, 1353, 1506, 2146
BfaI CTAG 5 cut(s) 296, 579, 624, 1344, 1862
BfmI CTRYAG 4 cut(s) 192, 864, 1521, 1851
BfoI RGCGCY 1 cut(s) 1101
BglII AGATCT 1 cut(s) 188
BisI GCNGC 9 cut(s) 50, 174, 362, 444, 452, 1064, 1071, 1387, 1477
BlnI CCTAGG 1 cut(s) 578
BlpI GCTNAGC 2 cut(s) 980, 1480
BlsI GCNGC 9 cut(s) 51, 175, 363, 445, 453, 1065, 1072, 1388, 1478
Bme1390I CCNGG 4 cut(s) 1052, 1109, 1565, 1690
Bme18I GGWCC 2 cut(s) 226, 1648
BmgT120I GGNCC 2 cut(s) 226, 1648
BmiI GGNNCC 8 cut(s) 42, 775, 932, 1131, 1569, 1585, 1721, 1991
BmrFI CCNGG 4 cut(s) 1052, 1109, 1565, 1690
BmrI ACTGGG 1 cut(s) 2365
BmsI GCATC 6 cut(s) 533, 892, 1045, 1046, 1932, 2047
BmuI ACTGGG 1 cut(s) 2365
BpiI GAAGAC 1 cut(s) 2272
BplI GAGNNNNNCTC 2 cut(s) 1436, 1468
BpmI CTGGAG 3 cut(s) 107, 533, 747
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu1102I GCTNAGC 2 cut(s) 980, 1480
Bpu14I TTCGAA 2 cut(s) 344, 1699
BpuEI CTTGAG 1 cut(s) 2036
BsaBI GATNNNNATC 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 6 cut(s) 112, 578, 1660, 1937, 1993, 2062
BsaXI ACNNNNNCTCC 4 cut(s) 678, 708, 1698, 1728
Bsc4I CCNNNNNNNGG 1 cut(s) 683
Bse1I ACTGG 7 cut(s) 124, 516, 571, 1723, 1804, 2202, 2360
Bse21I CCTNAGG 1 cut(s) 795
Bse3DI GCAATG 2 cut(s) 1058, 2420
Bse8I GATNNNNATC 1 cut(s) 65
BseBI CCWGG 4 cut(s) 1052, 1109, 1565, 1690
BseDI CCNNGG 6 cut(s) 112, 578, 1660, 1937, 1993, 2062
BseGI GGATG 3 cut(s) 66, 1060, 1947
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 1 cut(s) 683
BseMI GCAATG 2 cut(s) 1058, 2420
BseMII CTCAG 7 cut(s) 441, 632, 786, 819, 1005, 1494, 2338
BseNI ACTGG 7 cut(s) 124, 516, 571, 1723, 1804, 2202, 2360
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 7 cut(s) 61, 160, 373, 463, 1075, 1082, 1488
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 6 cut(s) 117, 323, 1376, 1386, 1563, 1998
BshNI GGYRCC 2 cut(s) 1129, 1989
BslFI GGGAC 3 cut(s) 1296, 1442, 1530
BslI CCNNNNNNNGG 1 cut(s) 683
BsmAI GTCTC 4 cut(s) 100, 1353, 1506, 2146
BsmBI CGTCTC 1 cut(s) 1506
BsmFI GGGAC 3 cut(s) 1296, 1442, 1530
BsnI GGCC 6 cut(s) 117, 323, 1376, 1386, 1563, 1998
Bsp119I TTCGAA 2 cut(s) 344, 1699
Bsp1286I GDGCHC 2 cut(s) 155, 2176
Bsp1720I GCTNAGC 2 cut(s) 980, 1480
Bsp19I CCATGG 2 cut(s) 1937, 2062
BspACI CCGC 5 cut(s) 444, 1066, 1147, 1387, 1588
BspANI GGCC 6 cut(s) 117, 323, 1376, 1386, 1563, 1998
BspCNI CTCAG 7 cut(s) 440, 633, 787, 820, 1006, 1493, 2337
BspFNI CGCG 1 cut(s) 446
BspLI GGNNCC 8 cut(s) 42, 775, 932, 1131, 1569, 1585, 1721, 1991
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 9 cut(s) 33, 527, 668, 807, 1042, 1562, 1575, 1578, 1591
BspQI GCTCTTC 1 cut(s) 143
BspT104I TTCGAA 2 cut(s) 344, 1699
BspT107I GGYRCC 2 cut(s) 1129, 1989
BsrDI GCAATG 2 cut(s) 1058, 2420
BsrI ACTGG 7 cut(s) 124, 516, 571, 1723, 1804, 2202, 2360
BssECI CCNNGG 6 cut(s) 112, 578, 1660, 1937, 1993, 2062
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 5 cut(s) 578, 1660, 1937, 1993, 2062
Bst2UI CCWGG 4 cut(s) 1052, 1109, 1565, 1690
Bst4CI ACNGT 7 cut(s) 94, 350, 879, 1470, 1754, 1897, 2296
Bst6I CTCTTC 2 cut(s) 143, 2022
BstACI GRCGYC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 1223
BstBI TTCGAA 2 cut(s) 344, 1699
BstC8I GCNNGC 7 cut(s) 205, 648, 813, 1068, 1492, 2036, 2232
BstDSI CCRYGG 2 cut(s) 1937, 2062
BstEII GGTNACC 1 cut(s) 107
BstENI CCTNNNNNAGG 1 cut(s) 681
BstF5I GGATG 3 cut(s) 66, 1060, 1947
BstFNI CGCG 1 cut(s) 446
BstH2I RGCGCY 1 cut(s) 1101
BstHHI GCGC 2 cut(s) 1100, 2291
BstMAI GTCTC 4 cut(s) 100, 1353, 1506, 2146
BstMWI GCNNNNNNNGC 6 cut(s) 49, 200, 773, 922, 1223, 1523
BstNI CCWGG 4 cut(s) 1052, 1109, 1565, 1690
BstNSI RCATGY 2 cut(s) 979, 1840
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 4 cut(s) 1050, 1107, 1563, 1688
BstSFI CTRYAG 4 cut(s) 192, 864, 1521, 1851
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 7 cut(s) 61, 160, 373, 463, 1075, 1082, 1488
BstV2I GAAGAC 1 cut(s) 2272
BstX2I RGATCY 6 cut(s) 25, 188, 799, 1047, 1567, 1583
BstXI CCANNNNNNTGG 1 cut(s) 2069
BstYI RGATCY 6 cut(s) 25, 188, 799, 1047, 1567, 1583
Bsu36I CCTNAGG 1 cut(s) 795
BsuRI GGCC 6 cut(s) 117, 323, 1376, 1386, 1563, 1998
BtgI CCRYGG 2 cut(s) 1937, 2062
BtsCI GGATG 3 cut(s) 66, 1060, 1947
BtsI GCAGTG 2 cut(s) 445, 1597
BtsIMutI CAGTG 9 cut(s) 90, 355, 445, 564, 1597, 1888, 2209, 2332, 2353
Cac8I GCNNGC 7 cut(s) 205, 648, 813, 1068, 1492, 2036, 2232
CfoI GCGC 2 cut(s) 1100, 2291
Cfr13I GGNCC 2 cut(s) 226, 1648
CseI GACGC 2 cut(s) 1523, 1532
Csp6I GTAC 4 cut(s) 1730, 1934, 2099, 2129
CviQI GTAC 4 cut(s) 1730, 1934, 2099, 2129
DraI TTTAAA 1 cut(s) 1749
DraIII CACNNNGTG 1 cut(s) 2359
EaeI YGGCCR 1 cut(s) 1384
Eam1104I CTCTTC 2 cut(s) 143, 2022
EarI CTCTTC 2 cut(s) 143, 2022
Eco130I CCWWGG 5 cut(s) 578, 1660, 1937, 1993, 2062
Eco24I GRGCYC 2 cut(s) 155, 2176
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 2 cut(s) 226, 1648
Eco81I CCTNAGG 1 cut(s) 795
Eco91I GGTNACC 1 cut(s) 107
EcoNI CCTNNNNNAGG 1 cut(s) 681
EcoO65I GGTNACC 1 cut(s) 107
EcoRI GAATTC 2 cut(s) 2044, 2363
EcoRII CCWGG 4 cut(s) 1050, 1107, 1563, 1688
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 5 cut(s) 578, 1660, 1937, 1993, 2062
EcoT22I ATGCAT 1 cut(s) 1060
EcoT38I GRGCYC 2 cut(s) 155, 2176
ErhI CCWWGG 5 cut(s) 578, 1660, 1937, 1993, 2062
Esp3I CGTCTC 1 cut(s) 1506
FaqI GGGAC 3 cut(s) 1296, 1442, 1530
FauI CCCGC 2 cut(s) 1059, 1595
FauNDI CATATG 2 cut(s) 1192, 1486
FbaI TGATCA 1 cut(s) 2253
Fnu4HI GCNGC 9 cut(s) 50, 174, 362, 444, 452, 1064, 1071, 1387, 1477
FokI GGATG 3 cut(s) 53, 1067, 1954
FriOI GRGCYC 2 cut(s) 155, 2176
Fsp4HI GCNGC 9 cut(s) 50, 174, 362, 444, 452, 1064, 1071, 1387, 1477
FspBI CTAG 5 cut(s) 296, 579, 624, 1344, 1862
FspI TGCGCA 1 cut(s) 2290
GlaI GCGC 2 cut(s) 1099, 2290
GluI GCNGC 9 cut(s) 50, 174, 362, 444, 452, 1064, 1071, 1387, 1477
GsuI CTGGAG 3 cut(s) 107, 533, 747
HaeII RGCGCY 1 cut(s) 1101
HaeIII GGCC 6 cut(s) 117, 323, 1376, 1386, 1563, 1998
HgaI GACGC 2 cut(s) 1523, 1532
HhaI GCGC 2 cut(s) 1100, 2291
Hin1I GRCGYC 1 cut(s) 279
Hin6I GCGC 2 cut(s) 1098, 2289
HinP1I GCGC 2 cut(s) 1098, 2289
HincII GTYRAC 2 cut(s) 277, 1711
HindII GTYRAC 2 cut(s) 277, 1711
HindIII AAGCTT 3 cut(s) 392, 765, 1352
HinfI GANTC 6 cut(s) 77, 467, 488, 607, 1024, 1079
HpaI GTTAAC 1 cut(s) 1711
HphI GGTGA 9 cut(s) 77, 119, 323, 425, 494, 661, 952, 1286, 1877
Hpy166II GTNNAC 4 cut(s) 277, 289, 1711, 1797
Hpy188I TCNGA 9 cut(s) 21, 40, 162, 642, 850, 875, 1350, 1890, 2194
Hpy8I GTNNAC 4 cut(s) 277, 289, 1711, 1797
Hpy99I CGWCG 1 cut(s) 2435
HpyAV CCTTC 5 cut(s) 261, 685, 940, 2171, 2345
HpyCH4III ACNGT 7 cut(s) 94, 350, 879, 1470, 1754, 1897, 2296
HpyCH4IV ACGT 4 cut(s) 214, 279, 1713, 2250
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 200, 773, 922, 1223, 1523
HpySE526I ACGT 4 cut(s) 214, 279, 1713, 2250
Hsp92I GRCGYC 1 cut(s) 279
HspAI GCGC 2 cut(s) 1098, 2289
Ksp22I TGATCA 1 cut(s) 2253
KspAI GTTAAC 1 cut(s) 1711
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 5 cut(s) 40, 514, 773, 913, 2135
Lsp1109I GCAGC 7 cut(s) 61, 160, 373, 463, 1075, 1082, 1488
LweI GCATC 6 cut(s) 533, 892, 1045, 1046, 1932, 2047
MaeI CTAG 5 cut(s) 296, 579, 624, 1344, 1862
MaeII ACGT 4 cut(s) 214, 279, 1713, 2250
MaeIII GTNAC 8 cut(s) 107, 667, 782, 897, 991, 1240, 1299, 2393
MflI RGATCY 6 cut(s) 25, 188, 799, 1047, 1567, 1583
MhlI GDGCHC 2 cut(s) 155, 2176
MlyI GAGTC 3 cut(s) 86, 1033, 1073
MmeI TCCRAC 2 cut(s) 18, 1107
Mph1103I ATGCAT 1 cut(s) 1060
MroXI GAANNNNTTC 1 cut(s) 844
MslI CAYNNNNRTG 5 cut(s) 480, 1370, 1489, 1784, 2067
MspA1I CMGCKG 2 cut(s) 1066, 1526
MspR9I CCNGG 4 cut(s) 1052, 1109, 1565, 1690
MvaI CCWGG 4 cut(s) 1052, 1109, 1565, 1690
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 6 cut(s) 49, 200, 773, 922, 1223, 1523
NcoI CCATGG 2 cut(s) 1937, 2062
NdeI CATATG 2 cut(s) 1192, 1486
NlaIV GGNNCC 8 cut(s) 42, 775, 932, 1131, 1569, 1585, 1721, 1991
NmeAIII GCCGAG 2 cut(s) 93, 349
NmuCI GTSAC 3 cut(s) 107, 667, 1240
NsbI TGCGCA 1 cut(s) 2290
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 2 cut(s) 979, 1840
NspV TTCGAA 2 cut(s) 344, 1699
PciI ACATGT 1 cut(s) 1836
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 844
PfeI GAWTC 3 cut(s) 467, 488, 607
PfoI TCCNGGA 1 cut(s) 1050
PkrI GCNGC 9 cut(s) 51, 175, 363, 445, 453, 1065, 1072, 1388, 1478
PleI GAGTC 3 cut(s) 85, 1032, 1073
PpsI GAGTC 3 cut(s) 85, 1032, 1073
PscI ACATGT 1 cut(s) 1836
Psp1406I AACGTT 1 cut(s) 1713
Psp6I CCWGG 4 cut(s) 1050, 1107, 1563, 1688
PspEI GGTNACC 1 cut(s) 107
PspGI CCWGG 4 cut(s) 1050, 1107, 1563, 1688
PspN4I GGNNCC 8 cut(s) 42, 775, 932, 1131, 1569, 1585, 1721, 1991
PspPI GGNCC 2 cut(s) 226, 1648
PsrI GAACNNNNNNTAC 2 cut(s) 2363, 2395
PstI CTGCAG 1 cut(s) 196
PsuI RGATCY 6 cut(s) 25, 188, 799, 1047, 1567, 1583
PvuII CAGCTG 1 cut(s) 1526
RsaI GTAC 4 cut(s) 1731, 1935, 2100, 2130
RsaNI GTAC 4 cut(s) 1730, 1934, 2099, 2129
RseI CAYNNNNRTG 5 cut(s) 480, 1370, 1489, 1784, 2067
SapI GCTCTTC 1 cut(s) 143
SatI GCNGC 9 cut(s) 50, 174, 362, 444, 452, 1064, 1071, 1387, 1477
Sau96I GGNCC 2 cut(s) 226, 1648
SchI GAGTC 3 cut(s) 86, 1033, 1073
ScrFI CCNGG 4 cut(s) 1052, 1109, 1565, 1690
SduI GDGCHC 2 cut(s) 155, 2176
SfaNI GCATC 6 cut(s) 533, 892, 1045, 1046, 1932, 2047
SfcI CTRYAG 4 cut(s) 192, 864, 1521, 1851
SfuI TTCGAA 2 cut(s) 344, 1699
SinI GGWCC 2 cut(s) 226, 1648
SmiMI CAYNNNNRTG 5 cut(s) 480, 1370, 1489, 1784, 2067
SmlI CTYRAG 1 cut(s) 2015
SmoI CTYRAG 1 cut(s) 2015
SsiI CCGC 5 cut(s) 444, 1066, 1147, 1387, 1588
SspI AATATT 1 cut(s) 907
SspMI CTAG 5 cut(s) 296, 579, 624, 1344, 1862
StyD4I CCNGG 4 cut(s) 1050, 1107, 1563, 1688
StyI CCWWGG 5 cut(s) 578, 1660, 1937, 1993, 2062
TaaI ACNGT 7 cut(s) 94, 350, 879, 1470, 1754, 1897, 2296
TaiI ACGT 4 cut(s) 217, 282, 1716, 2253
TaqI TCGA 4 cut(s) 75, 344, 616, 1699
TatI WGTACW 1 cut(s) 2128
TauI GCSGC 2 cut(s) 446, 1389
TfiI GAWTC 3 cut(s) 467, 488, 607
TscAI CASTG 9 cut(s) 97, 355, 445, 571, 1597, 1888, 2209, 2332, 2360
TseFI GTSAC 3 cut(s) 107, 667, 1240
TseI GCWGC 7 cut(s) 49, 173, 361, 451, 1063, 1070, 1476
Tsp45I GTSAC 3 cut(s) 107, 667, 1240
TspGWI ACGGA 2 cut(s) 1762, 2397
TspRI CASTG 9 cut(s) 97, 355, 445, 571, 1597, 1888, 2209, 2332, 2360
VpaK11BI GGWCC 2 cut(s) 226, 1648
XagI CCTNNNNNAGG 1 cut(s) 681
XceI RCATGY 2 cut(s) 979, 1840
XcmI CCANNNNNNNNNTGG 3 cut(s) 1658, 1880, 2060
XmaJI CCTAGG 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 844
XspI CTAG 5 cut(s) 296, 579, 624, 1344, 1862
ZraI GACGTC 1 cut(s) 280
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.