Rh6BG478200

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003618
Physical Location & Seq
Reverse (-)
68423790 .. 68429856
6067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG478200.1

Sequence Viewer

Length: 2118 bp
ATGAATTCAAACAATGAACAATTACAACGCACTTATAGTGAGCTATTAGAATACAAGCTTGTACTGCAGAAGGCTGGCGGGTTTTTTAGTTCAGCTCAAAGTAGTGCCACAGCTCAGCAAAGAGAAATTGAAGTGCAGCCCCTGGGTGAAAGATCCATTGACAGTCCATTATTACTCGAACAAGAAATGACAACAGATCCATCGAAGCATGTAAAGCTTGGGTCAGTGAGTGGTCTTGTTCCAAGAGAAAAGTCTTTGGCATTTGAAAGGATTTTATTTCGTGCCACCAGGGGTAATGTATTTCTGAAGCAAGCTGTGGTCAATGGCCCTGTTGTTGATCCTGTGTCAGGAGAGAAGGTTGAGAAAAACGTATTCATCATCTTCTATTCTGGCGAAAGGGCAAAGAATAAAATTCTAAAAATATGTGAGGCTTTTAGAGCAAACCGTTACCCATTTACAGATGACTTGGGAAAACAATTTCAGATGATTACAGAGGTGTCTGGAAAACTTTCAGAGCTGAAGTCTACCATAGATGCTGGACTATTGCACCGGAACAGTTTATTACAGACAATTGGCCATCAATATGAGCAATGGAACCTTCTGGTAAAGAAGGAAAAATCCGTCTACCACACCTTAAACATGCTCAGCATTGATGTGACAAAGATGTGTCTTGTTGCAGAAGGTTGGTGTCCTGTATCCGCATCAATTCAGATCCAAAACGCATTGCAGCAGGCAACCTTTGATACCAACTGTCAAGTTGGGGCCATATTCCAGGTTTTGCAGACAAAGGAATCGCCACCAACATATTTCCGTACAAACAAATTTACTTCTGCTTTTCAAGAAATTGTTGACGCATATGGGGTTGCCAAGTATCGGGAGGCTAATCCTGGTGTATACACCATTGTCACTTTCCCTTTCCTTTTCGCTGTGATGTTTGGAGATTGGGGGCATGGTATTTGCTTGTTGCTGGCAACACTATATTTCATCATCGGGGAGAGGAAGTTTTCTAGTCAGAAACTTGGGGACATTATTGAAATGACGTTTGGTGGCCGTTATGTTATTTTTATGATGGCGCTGTTCTCAATTTACACAGGCTTGATATATAATGAATTCTTTTCAGTCCCATTTGAAATATTTGGGCCTTCTGCCTATGGGTGCCGTGACCCATCCTGCAGGGATGCCACTACAGCAGGTTTGACTAAGGTGCGTGACACCTATCCATTTGGTGTGGATCCTAAATGGCACGGTTCCAGGTCTGAATTACCCTTTCTTAACTCGTTGAAAATGAAGATGTCAATCCTACTTGGAGTAGCCCAGATGAATCTTGGAATTGTATTAAGTTACTTCAATGCAAAATATTTTGCGGATAAGCTGAATATCTGGTACCAATTTGTTCCTCAGATGATATTTCTAAACAGTCTGTTTGGCTACCTTTCACTCCTCATTATTGTGAAATGGTGCACTGGTTCAAAAGCTGATTTGTATCATGTAATGATATACATGTTCCTGAGTCCTTTGGAAGATCTGGGTGAAAATCAGCTCTTTTGGGGGCAAGGATTCCTTCAGGTTCTATTATTACTATCCGCTCTTGTTGCTGTGCCATGGATGCTGTTTCCAAAGCCTTATTTCTTGAAAAAGCAGCATGAGGAGAGGCACCAAGGTCAATCATACGCGTTGCTTCTGACTGGGGAGGACCCTCTTGAAGATGACCAGGAAGACAATCACAATTCAAAAGGTCATGAGGAATTTGAATTCAGCGAGATCTTTGTACACCAACTCATACATACCATAGAGTTTGTGCTTGGAGCAGTGTCCAATACAGCTTCATATCTACGTTTATGGGCACTCAGCTTAGCACATTCAGAGTTGTCCAGTGTATTTTACGACAAGGTTCTGCTTCTAGCATGGGGGTTCAACAATGTCATTATTCTAATTATTGGCATAATCGTTTTTATAAGTGCAACTGTTGGAGTGCTACTAGTGATGGAGACCTTAAGTGCATTCTTGCATGCCTTGAGGCTTCACTGGGTGGAGTTTCAGAACAAGTTTTACGAGGGCGCTGGTTACAAATTCTGCCCTTTTTCATTTGCATTACTCGGTAGTGAGGATGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

705

Amino Acids

79.93

Weight (kDa)

5.94

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 1 - 696 6.9e-279 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1955
Acc36I ACCTGC 1 cut(s) 1181
Acc65I GGTACC 1 cut(s) 1383
AccB1I GGYRCC 3 cut(s) 1155, 1383, 1653
AccBSI CCGCTC 1 cut(s) 1586
AccI GTMKAC 3 cut(s) 524, 624, 894
AccII CGCG 1 cut(s) 1673
AciI CCGC 4 cut(s) 78, 699, 1364, 1584
AclWI GGATC 6 cut(s) 147, 191, 332, 706, 1226, 1239
AcoI YGGCCR 2 cut(s) 574, 1048
AcsI RAATTY 7 cut(s) 4, 411, 821, 1109, 1745, 1751, 2069
AcuI CTGAAG 3 cut(s) 326, 539, 1547
AdeI CACNNNGTG 1 cut(s) 2029
AfaI GTAC 4 cut(s) 63, 814, 1385, 1770
AfiI CCNNNNNNNGG 2 cut(s) 347, 873
AflII CTTAAG 1 cut(s) 1993
AflIII ACRYGT 2 cut(s) 1500, 1671
AhlI ACTAGT 1 cut(s) 1978
AjnI CCWGG 6 cut(s) 141, 287, 771, 886, 1250, 1710
AjuI GAANNNNNNNTTGG 2 cut(s) 1026, 1058
Alw21I GWGCWC 1 cut(s) 1463
Alw26I GTCTC 1 cut(s) 1982
Alw44I GTGCAC 1 cut(s) 1459
AlwI GGATC 6 cut(s) 147, 191, 332, 706, 1226, 1239
AlwNI CAGNNNCTG 1 cut(s) 142
AoxI GGCC 5 cut(s) 325, 574, 762, 1048, 1139
ApaLI GTGCAC 1 cut(s) 1459
ApeKI GCWGC 3 cut(s) 136, 727, 1639
ApoI RAATTY 7 cut(s) 4, 411, 821, 1109, 1745, 1751, 2069
Asp700I GAANNNNTTC 1 cut(s) 508
Asp718I GGTACC 1 cut(s) 1383
AspLEI GCGC 2 cut(s) 1075, 2060
AspS9I GGNCC 4 cut(s) 326, 762, 1139, 1693
AsuHPI GGTGA 2 cut(s) 158, 1541
AvaII GGWCC 1 cut(s) 1693
BaeGI GKGCMC 2 cut(s) 1463, 1846
BalI TGGCCA 1 cut(s) 576
BamHI GGATCC 1 cut(s) 1231
BanI GGYRCC 3 cut(s) 1155, 1383, 1653
BbsI GAAGAC 1 cut(s) 1722
Bbv12I GWGCWC 1 cut(s) 1463
BbvI GCAGC 3 cut(s) 148, 739, 1651
BccI CCATC 5 cut(s) 208, 585, 1063, 1174, 1978
BceAI ACGGC 2 cut(s) 1035, 1143
BciT130I CCWGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
BciVI GTATCC 1 cut(s) 706
BcoDI GTCTC 1 cut(s) 1982
BcuI ACTAGT 1 cut(s) 1978
BfaI CTAG 3 cut(s) 1008, 1901, 1979
BfmI CTRYAG 3 cut(s) 65, 1171, 1185
BfoI RGCGCY 2 cut(s) 1076, 2061
BfrI CTTAAG 1 cut(s) 1993
BfuAI ACCTGC 1 cut(s) 1181
BfuI GTATCC 1 cut(s) 706
BglII AGATCT 2 cut(s) 1522, 1761
BisI GCNGC 3 cut(s) 137, 728, 1640
BlpI GCTNAGC 3 cut(s) 114, 644, 1852
BlsI GCNGC 3 cut(s) 138, 729, 1641
Bme1390I CCNGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
Bme18I GGWCC 1 cut(s) 1693
BmgT120I GGNCC 4 cut(s) 326, 762, 1139, 1693
BmiI GGNNCC 8 cut(s) 596, 763, 1157, 1233, 1249, 1385, 1655, 1695
BmrFI CCNGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
BmrI ACTGGG 2 cut(s) 1695, 2035
BmsI GCATC 4 cut(s) 523, 710, 1168, 1596
BmuI ACTGGG 2 cut(s) 1695, 2035
BpiI GAAGAC 1 cut(s) 1722
Bpu1102I GCTNAGC 3 cut(s) 114, 644, 1852
BpuEI CTTGAG 1 cut(s) 2035
BsaBI GATNNNNATC 2 cut(s) 1295, 1482
BsaI GGTCTC 1 cut(s) 1982
BsaJI CCNNGG 5 cut(s) 141, 142, 288, 1601, 1657
BsaWI WCCGGW 1 cut(s) 549
BsaXI ACNNNNNCTCC 4 cut(s) 342, 372, 986, 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 873
Bse1I ACTGG 4 cut(s) 1468, 1690, 1872, 2030
Bse3DI GCAATG 2 cut(s) 596, 722
Bse8I GATNNNNATC 2 cut(s) 1295, 1482
BseBI CCWGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
BseDI CCNNGG 5 cut(s) 141, 142, 288, 1601, 1657
BseGI GGATG 4 cut(s) 1166, 1183, 1611, 2113
BseJI GATNNNNATC 2 cut(s) 1295, 1482
BseLI CCNNNNNNNGG 2 cut(s) 347, 873
BseMI GCAATG 2 cut(s) 596, 722
BseMII CTCAG 5 cut(s) 128, 658, 1412, 1499, 1861
BseNI ACTGG 4 cut(s) 1468, 1690, 1872, 2030
BseRI GAGGAG 2 cut(s) 1430, 1661
BseSI GKGCMC 2 cut(s) 1463, 1846
BseXI GCAGC 3 cut(s) 148, 739, 1651
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 1 cut(s) 1673
BshFI GGCC 5 cut(s) 327, 576, 764, 1050, 1141
BshNI GGYRCC 3 cut(s) 1155, 1383, 1653
BsiHKAI GWGCWC 1 cut(s) 1463
BsiSI CCGG 1 cut(s) 550
BslFI GGGAC 2 cut(s) 1037, 1106
BslI CCNNNNNNNGG 2 cut(s) 347, 873
BsmAI GTCTC 1 cut(s) 1982
BsmFI GGGAC 2 cut(s) 1037, 1106
BsmI GAATGC 1 cut(s) 2000
BsnI GGCC 5 cut(s) 327, 576, 764, 1050, 1141
Bso31I GGTCTC 1 cut(s) 1982
Bsp1286I GDGCHC 2 cut(s) 1463, 1846
Bsp1407I TGTACA 1 cut(s) 1768
Bsp143I GATC 7 cut(s) 152, 196, 337, 711, 1231, 1522, 1761
Bsp1720I GCTNAGC 3 cut(s) 114, 644, 1852
Bsp19I CCATGG 1 cut(s) 1601
BspACI CCGC 4 cut(s) 78, 699, 1364, 1584
BspANI GGCC 5 cut(s) 327, 576, 764, 1050, 1141
BspCNI CTCAG 5 cut(s) 127, 657, 1411, 1500, 1860
BspFNI CGCG 1 cut(s) 1673
BspHI TCATGA 1 cut(s) 1738
BspLI GGNNCC 8 cut(s) 596, 763, 1157, 1233, 1249, 1385, 1655, 1695
BspMAI CTGCAG 2 cut(s) 69, 1175
BspMI ACCTGC 1 cut(s) 1181
BspPI GGATC 6 cut(s) 147, 191, 332, 706, 1226, 1239
BspT107I GGYRCC 3 cut(s) 1155, 1383, 1653
BspTI CTTAAG 1 cut(s) 1993
BspTNI GGTCTC 1 cut(s) 1982
BsrBI CCGCTC 1 cut(s) 1586
BsrDI GCAATG 2 cut(s) 596, 722
BsrGI TGTACA 1 cut(s) 1768
BsrI ACTGG 4 cut(s) 1468, 1690, 1872, 2030
BssECI CCNNGG 5 cut(s) 141, 142, 288, 1601, 1657
BssMI GATC 7 cut(s) 152, 196, 337, 711, 1231, 1522, 1761
BssNAI GTATAC 1 cut(s) 895
BssT1I CCWWGG 2 cut(s) 1601, 1657
Bst1107I GTATAC 1 cut(s) 895
Bst2UI CCWGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
Bst4CI ACNGT 7 cut(s) 164, 446, 557, 752, 1247, 1418, 1966
BstAFI CTTAAG 1 cut(s) 1993
BstAUI TGTACA 1 cut(s) 1768
BstC8I GCNNGC 5 cut(s) 76, 312, 732, 969, 2010
BstDEI CTNAG 7 cut(s) 114, 644, 1200, 1398, 1508, 1847, 1852
BstDSI CCRYGG 1 cut(s) 1601
BstENI CCTNNNNNAGG 1 cut(s) 345
BstF5I GGATG 4 cut(s) 1166, 1183, 1611, 2113
BstFNI CGCG 1 cut(s) 1673
BstH2I RGCGCY 2 cut(s) 1076, 2061
BstHHI GCGC 2 cut(s) 1075, 2060
BstKTI GATC 7 cut(s) 155, 199, 340, 714, 1234, 1525, 1764
BstMAI GTCTC 1 cut(s) 1982
BstMBI GATC 7 cut(s) 152, 196, 337, 711, 1231, 1522, 1761
BstMWI GCNNNNNNNGC 6 cut(s) 64, 214, 437, 1187, 1592, 1606
BstNI CCWGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
BstNSI RCATGY 4 cut(s) 212, 643, 1504, 2012
BstSCI CCNGG 6 cut(s) 141, 287, 771, 886, 1250, 1710
BstSFI CTRYAG 3 cut(s) 65, 1171, 1185
BstSLI GKGCMC 2 cut(s) 1463, 1846
BstUI CGCG 1 cut(s) 1673
BstV1I GCAGC 3 cut(s) 148, 739, 1651
BstV2I GAAGAC 1 cut(s) 1722
BstX2I RGATCY 6 cut(s) 152, 196, 711, 1231, 1522, 1761
BstYI RGATCY 6 cut(s) 152, 196, 711, 1231, 1522, 1761
BstZ17I GTATAC 1 cut(s) 895
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 5 cut(s) 327, 576, 764, 1050, 1141
BtgI CCRYGG 1 cut(s) 1601
BtsCI GGATG 4 cut(s) 1166, 1183, 1611, 2113
BtsI GCAGTG 1 cut(s) 1815
BtsIMutI CAGTG 5 cut(s) 231, 1461, 1815, 1879, 2023
BveI ACCTGC 1 cut(s) 1181
Cac8I GCNNGC 5 cut(s) 76, 312, 732, 969, 2010
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 1 cut(s) 1738
CfoI GCGC 2 cut(s) 1075, 2060
Cfr13I GGNCC 4 cut(s) 326, 762, 1139, 1693
CseI GACGC 1 cut(s) 860
Csp6I GTAC 4 cut(s) 62, 813, 1384, 1769
CviQI GTAC 4 cut(s) 62, 813, 1384, 1769
DdeI CTNAG 7 cut(s) 114, 644, 1200, 1398, 1508, 1847, 1852
DpnI GATC 7 cut(s) 154, 198, 339, 713, 1233, 1524, 1763
DpnII GATC 7 cut(s) 152, 196, 337, 711, 1231, 1522, 1761
DraIII CACNNNGTG 1 cut(s) 2029
EaeI YGGCCR 2 cut(s) 574, 1048
Eco130I CCWWGG 2 cut(s) 1601, 1657
Eco31I GGTCTC 1 cut(s) 1982
Eco47I GGWCC 1 cut(s) 1693
Eco57I CTGAAG 3 cut(s) 326, 539, 1547
EcoNI CCTNNNNNAGG 1 cut(s) 345
EcoO109I RGGNCCY 1 cut(s) 1693
EcoRI GAATTC 3 cut(s) 4, 1109, 1751
EcoRII CCWGG 6 cut(s) 141, 287, 771, 886, 1250, 1710
EcoT14I CCWWGG 2 cut(s) 1601, 1657
ErhI CCWWGG 2 cut(s) 1601, 1657
FalI AAGNNNNNCTT 2 cut(s) 1545, 1577
FaqI GGGAC 2 cut(s) 1037, 1106
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 3 cut(s) 524, 624, 894
Fnu4HI GCNGC 3 cut(s) 137, 728, 1640
FokI GGATG 3 cut(s) 1153, 1190, 1618
Fsp4HI GCNGC 3 cut(s) 137, 728, 1640
FspBI CTAG 3 cut(s) 1008, 1901, 1979
GlaI GCGC 2 cut(s) 1074, 2059
GluI GCNGC 3 cut(s) 137, 728, 1640
HaeII RGCGCY 2 cut(s) 1076, 2061
HaeIII GGCC 5 cut(s) 327, 576, 764, 1050, 1141
HapII CCGG 1 cut(s) 550
HgaI GACGC 1 cut(s) 860
HhaI GCGC 2 cut(s) 1075, 2060
Hin6I GCGC 2 cut(s) 1073, 2058
HinP1I GCGC 2 cut(s) 1073, 2058
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 2 cut(s) 56, 215
HinfI GANTC 4 cut(s) 791, 1321, 1510, 1557
HpaII CCGG 1 cut(s) 550
HphI GGTGA 2 cut(s) 158, 1541
Hpy166II GTNNAC 6 cut(s) 525, 625, 850, 895, 1461, 1771
Hpy188III TCNNGA 8 cut(s) 348, 501, 839, 875, 1507, 1630, 1700, 1739
Hpy8I GTNNAC 6 cut(s) 525, 625, 850, 895, 1461, 1771
HpyAV CCTTC 7 cut(s) 64, 349, 604, 608, 674, 1152, 1571
HpyCH4III ACNGT 7 cut(s) 164, 446, 557, 752, 1247, 1418, 1966
HpyCH4IV ACGT 3 cut(s) 369, 1040, 1834
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 214, 437, 1187, 1592, 1606
HpyF3I CTNAG 7 cut(s) 114, 644, 1200, 1398, 1508, 1847, 1852
HpySE526I ACGT 3 cut(s) 369, 1040, 1834
HspAI GCGC 2 cut(s) 1073, 2058
KpnI GGTACC 1 cut(s) 1387
Kzo9I GATC 7 cut(s) 152, 196, 337, 711, 1231, 1522, 1761
LmnI GCTCC 1 cut(s) 1805
Lsp1109I GCAGC 3 cut(s) 148, 739, 1651
LweI GCATC 4 cut(s) 523, 710, 1168, 1596
MaeI CTAG 3 cut(s) 1008, 1901, 1979
MaeII ACGT 3 cut(s) 369, 1040, 1834
MaeIII GTNAC 7 cut(s) 446, 655, 904, 1160, 1208, 1340, 2063
MalI GATC 7 cut(s) 154, 198, 339, 713, 1233, 1524, 1763
MbiI CCGCTC 1 cut(s) 1586
MboI GATC 7 cut(s) 152, 196, 337, 711, 1231, 1522, 1761
MboII GAAGA 5 cut(s) 373, 1300, 1532, 1715, 1727
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 6 cut(s) 152, 196, 711, 1231, 1522, 1761
MhlI GDGCHC 2 cut(s) 1463, 1846
MlsI TGGCCA 1 cut(s) 576
MluI ACGCGT 1 cut(s) 1671
MluNI TGGCCA 1 cut(s) 576
MlyI GAGTC 1 cut(s) 1519
MmeI TCCRAC 1 cut(s) 1948
Mox20I TGGCCA 1 cut(s) 576
MroXI GAANNNNTTC 1 cut(s) 508
MscI TGGCCA 1 cut(s) 576
MseI TTAA 4 cut(s) 635, 1272, 1337, 1994
MslI CAYNNNNRTG 3 cut(s) 582, 653, 1448
Msp20I TGGCCA 1 cut(s) 576
MspCI CTTAAG 1 cut(s) 1993
MspI CCGG 1 cut(s) 550
MspR9I CCNGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
MunI CAATTG 1 cut(s) 570
Mva1269I GAATGC 1 cut(s) 2000
MvaI CCWGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
MvnI CGCG 1 cut(s) 1673
MwoI GCNNNNNNNGC 6 cut(s) 64, 214, 437, 1187, 1592, 1606
NcoI CCATGG 1 cut(s) 1601
NdeI CATATG 1 cut(s) 856
NdeII GATC 7 cut(s) 152, 196, 337, 711, 1231, 1522, 1761
NlaIV GGNNCC 8 cut(s) 596, 763, 1157, 1233, 1249, 1385, 1655, 1695
NmuCI GTSAC 4 cut(s) 655, 904, 1160, 1208
NspI RCATGY 4 cut(s) 212, 643, 1504, 2012
PaeI GCATGC 1 cut(s) 2012
PagI TCATGA 1 cut(s) 1738
PasI CCCWGGG 1 cut(s) 142
PciI ACATGT 1 cut(s) 1500
PctI GAATGC 1 cut(s) 2000
PdmI GAANNNNTTC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 791, 1321, 1557
PkrI GCNGC 3 cut(s) 138, 729, 1641
PleI GAGTC 1 cut(s) 1518
PpsI GAGTC 1 cut(s) 1518
PpuMI RGGWCCY 1 cut(s) 1693
PscI ACATGT 1 cut(s) 1500
PsiI TTATAA 1 cut(s) 1955
Psp5II RGGWCCY 1 cut(s) 1693
Psp6I CCWGG 6 cut(s) 141, 287, 771, 886, 1250, 1710
PspGI CCWGG 6 cut(s) 141, 287, 771, 886, 1250, 1710
PspN4I GGNNCC 8 cut(s) 596, 763, 1157, 1233, 1249, 1385, 1655, 1695
PspPI GGNCC 4 cut(s) 326, 762, 1139, 1693
PspPPI RGGWCCY 1 cut(s) 1693
PsrI GAACNNNNNNTAC 2 cut(s) 2033, 2065
PstI CTGCAG 2 cut(s) 69, 1175
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 6 cut(s) 152, 196, 711, 1231, 1522, 1761
RsaI GTAC 4 cut(s) 63, 814, 1385, 1770
RsaNI GTAC 4 cut(s) 62, 813, 1384, 1769
RseI CAYNNNNRTG 3 cut(s) 582, 653, 1448
SaqAI TTAA 4 cut(s) 635, 1272, 1337, 1994
SatI GCNGC 3 cut(s) 137, 728, 1640
Sau3AI GATC 7 cut(s) 152, 196, 337, 711, 1231, 1522, 1761
Sau96I GGNCC 4 cut(s) 326, 762, 1139, 1693
SbfI CCTGCAGG 1 cut(s) 1175
SchI GAGTC 1 cut(s) 1519
ScrFI CCNGG 6 cut(s) 143, 289, 773, 888, 1252, 1712
SdaI CCTGCAGG 1 cut(s) 1175
SduI GDGCHC 2 cut(s) 1463, 1846
SfaNI GCATC 4 cut(s) 523, 710, 1168, 1596
SfcI CTRYAG 3 cut(s) 65, 1171, 1185
SinI GGWCC 1 cut(s) 1693
SmiMI CAYNNNNRTG 3 cut(s) 582, 653, 1448
SmlI CTYRAG 2 cut(s) 1993, 2014
SmoI CTYRAG 2 cut(s) 1993, 2014
SpeI ACTAGT 1 cut(s) 1978
SphI GCATGC 1 cut(s) 2012
Sse8387I CCTGCAGG 1 cut(s) 1175
SsiI CCGC 4 cut(s) 78, 699, 1364, 1584
SspI AATATT 2 cut(s) 1134, 1358
SspMI CTAG 3 cut(s) 1008, 1901, 1979
StyD4I CCNGG 6 cut(s) 141, 287, 771, 886, 1250, 1710
StyI CCWWGG 2 cut(s) 1601, 1657
TaaI ACNGT 7 cut(s) 164, 446, 557, 752, 1247, 1418, 1966
TaiI ACGT 3 cut(s) 372, 1043, 1837
TaqI TCGA 2 cut(s) 177, 203
TatI WGTACW 2 cut(s) 61, 1768
TfiI GAWTC 3 cut(s) 791, 1321, 1557
Tru1I TTAA 4 cut(s) 635, 1272, 1337, 1994
Tru9I TTAA 4 cut(s) 635, 1272, 1337, 1994
TscAI CASTG 5 cut(s) 231, 1468, 1815, 1879, 2030
TseFI GTSAC 4 cut(s) 655, 904, 1160, 1208
TseI GCWGC 3 cut(s) 136, 727, 1639
Tsp45I GTSAC 4 cut(s) 655, 904, 1160, 1208
TspDTI ATGAA 9 cut(s) 17, 30, 364, 973, 1122, 1301, 1334, 1815, 2073
TspGWI ACGGA 2 cut(s) 610, 800
TspRI CASTG 5 cut(s) 231, 1468, 1815, 1879, 2030
Vha464I CTTAAG 1 cut(s) 1993
VneI GTGCAC 1 cut(s) 1459
VpaK11BI GGWCC 1 cut(s) 1693
XagI CCTNNNNNAGG 1 cut(s) 345
XapI RAATTY 7 cut(s) 4, 411, 821, 1109, 1745, 1751, 2069
XceI RCATGY 4 cut(s) 212, 643, 1504, 2012
XcmI CCANNNNNNNNNTGG 1 cut(s) 1322
XmiI GTMKAC 3 cut(s) 524, 624, 894
XmnI GAANNNNTTC 1 cut(s) 508
XspI CTAG 3 cut(s) 1008, 1901, 1979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.