Rh6DG424300

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003618
Physical Location & Seq
Forward (+)
60518660 .. 60524637
5978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG424300.1

Sequence Viewer

Length: 2463 bp
ATGGTGACGGAGGATTGTTGGCCAACAATGGATCTACTGCGGTCGGAGCCAATGCAACTGGTGCACCTCATCATTCCGATCGAATCGTCTCGCCGGGCCATCTCCTACCTCGGCGACCTTGGCCTTTTTCAATTCAAAGATCTCAATGCGGAGAAGAGTCCGTTCCAGAGAACATATGCCGCTCAGATCAAACGATGTGGGGAAATGGCTCGAAGGTTACGTTTCTTCAGAGATCAGATGAAGAAGGCTGGTTTGTCACATTCAACAATGTCTACAATGAGCGATGATAATGATCTTGATCGCTTGGAGGTTAAACTCAGTGAACTTGAAGCTGATCTATTAGAAATGAATTCAAACAATGAACAATTACAACGCACTTATAGTGAGCTATTAGAATACAAGCTTGTACTGCAGAAGGCTGGCGGGTTTTTTAGTTCAGCTCAAAGTAGTGCCACAGCTCAGCAAAGAGAAATTGAAGTGCAGCCCCTGGGTGAAAGATCCATTGACAGTCCATTATTACTCGAACAAGAAATGACAACAGATCCATCGAAGCATGTAAAGCTTGGGTCAGTGAGTGGTCTTGTTCCAAGAGAAAAGTCTTTGGCATTTGAAAGGATTTTATTTCGTGCCACCAGGGGTAATGTATTTCTGAAGCAAGCTGTGGTCAATGGCCCTGTTGTTGATCCTGTGTCAGGAGAGAAGGTTGAGAAAAACGTATTCATCATCTTCTATTCTGGCGAAAGGGCAAAGAATAAAATTCTAAAAATATGTGAGGCTTTTAGAGCAAACCGTTACCCATTTACAGATGACTTGGGAAAACAATTTCAGATGATTACAGAGGTGTCTGGAAAACTTTCAGAGCTGAAGTCTACCATAGATGCTGGACTATTGCACCGGAACAGTTTATTACAGACAATTGGCCATCAATATGAGCAATGGAACCTTCTGGTAAAGAAGGAAAAATCCGTCTACCACACCTTAAACATGCTCAGCATTGATGTGACAAAGATGTGTCTTGTTGCAGAAGGTTGGTGTCCTGTATCCGCATCAATTCAGATCCAAAACGCATTGCAGCAGGCAACCTTTGATACCAACTGTCAAGTTGGGGCCATATTCCAGGTTTTGCAGACAAAGGAATCGCCACCAACATATTTCCGTACAAACAAATTTACTTCTGCTTTTCAAGAAATTGTTGACGCATATGGGGTTGCCAAGTATCGGGAGGCTAATCCTGGTGTATACACCATTGTCACTTTCCCTTTCCTTTTCGCTGTGATGTTTGGAGATTGGGGGCATGGTATTTGCTTGTTGCTGGCAACACTATATTTCATCATCAGGGAGAGGAAGTTTTCTAGTCAGAAACTTGGGGACATTATTGAAATGACGTTTGGTGGCCGTTATGTTATTTTTATGATGGCGCTGTTCTCAATTTACACAGGCTTGATATATAATGAATTCTTTTCAGTCCCATTTGAAATATTTGGGCCTTCTGCCTATGGGTGCCGTGACCCATCCTGCAGGGATGCCACTACAGCAGGTTTGACTAAGGTGCGTGACACCTATCCATTTGGTGTGGATCCTAAATGGCACGGTTCCAGGTCTGAATTACCCTTTCTTAACTCGTTGAAAATGAAGATGTCAATCCTACTTGGAGTAGCCCAGATGAATCTTGGAATTGTATTAAGTTACTTCAATGCAAAATATTTTGCGGATAAGCTGAATATCTGGTACCAATTTGTTCCTCAGATGATATTTCTAAACAGTCTGTTTGGCTACCTTTCACTCCTCATTATTGTGAAATGGTGCACTGGTTCAAAAGCTGATTTGTATCATGTAATGATATACATGTTCCTGAGTCCTTTCGAAGATCTGGGTGAAAATCAGCTCTTTTGGGGGCAAGGATTCCTTCAGGTTCTATTATTACTATCCGCTCTTGTTGCTGTGCCATGGATGCTGTTTCCAAAGCCTTATTTCTTGAAAAAGCAGCATGAGGAGAGGCACCAAGGTCAATCATACGCGTTGCTTCTGACTGGGGAGGACCCTCTTGAAGATGACCAGGAAGACAATCACAATTCAAAAGGTCATGAGGAATTTGAATTCAGCGAGATCTTTGTACACCAACTCATACATACCATAGAGTTTGTGCTTGGAGCAGTGTCCAATACAGCTTCATATCTACGTTTATGGGCACTCAGCTTAGCACATTCAGAGTTGTCCAGTGTATTTTACGACAAGGTTCTGCTTCTAGCATGGGGGTTCAACAATGTCATTATTCTAATTATTGGCATAATCGTTTTTATAAGTGCAACTGTTGGAGTGCTACTAGTGATGGAGACCTTAAGTGCATTCTTGCATGCCTTGAGGCTTCACTGGGTGGAGTTTCAGAACAAGTTTTACGAGGGCGCTGGTTACAAATTCTGCCCTTTTTCATTTGCATTACTCGGTAGTGAGGATGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

820

Amino Acids

93.39

Weight (kDa)

5.91

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 36 - 811 9.7e-304 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2300
Acc36I ACCTGC 1 cut(s) 1526
Acc65I GGTACC 1 cut(s) 1728
AccB1I GGYRCC 3 cut(s) 1500, 1728, 1998
AccBSI CCGCTC 2 cut(s) 182, 1931
AccI GTMKAC 4 cut(s) 272, 869, 969, 1239
AccII CGCG 1 cut(s) 2018
AciI CCGC 7 cut(s) 40, 149, 180, 423, 1044, 1709, 1929
AclWI GGATC 7 cut(s) 39, 492, 536, 677, 1051, 1571, 1584
AcoI YGGCCR 3 cut(s) 20, 919, 1393
AcsI RAATTY 7 cut(s) 349, 756, 1166, 1454, 2090, 2096, 2414
AcuI CTGAAG 4 cut(s) 211, 671, 884, 1892
AdeI CACNNNGTG 1 cut(s) 2374
AfaI GTAC 4 cut(s) 408, 1159, 1730, 2115
AfiI CCNNNNNNNGG 2 cut(s) 692, 1218
AflII CTTAAG 1 cut(s) 2338
AflIII ACRYGT 2 cut(s) 1845, 2016
AhlI ACTAGT 1 cut(s) 2323
AjnI CCWGG 6 cut(s) 486, 632, 1116, 1231, 1595, 2055
AjuI GAANNNNNNNTTGG 2 cut(s) 1371, 1403
Alw21I GWGCWC 2 cut(s) 66, 1808
Alw26I GTCTC 2 cut(s) 93, 2327
Alw44I GTGCAC 2 cut(s) 62, 1804
AlwI GGATC 7 cut(s) 39, 492, 536, 677, 1051, 1571, 1584
AlwNI CAGNNNCTG 1 cut(s) 487
AoxI GGCC 8 cut(s) 20, 96, 121, 670, 919, 1107, 1393, 1484
ApaLI GTGCAC 2 cut(s) 62, 1804
ApeKI GCWGC 3 cut(s) 481, 1072, 1984
ApoI RAATTY 7 cut(s) 349, 756, 1166, 1454, 2090, 2096, 2414
Asp700I GAANNNNTTC 1 cut(s) 853
Asp718I GGTACC 1 cut(s) 1728
AspLEI GCGC 2 cut(s) 1420, 2405
AspS9I GGNCC 5 cut(s) 96, 671, 1107, 1484, 2038
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 3 cut(s) 16, 503, 1886
AsuII TTCGAA 1 cut(s) 1863
AvaII GGWCC 1 cut(s) 2038
BaeGI GKGCMC 3 cut(s) 66, 1808, 2191
BalI TGGCCA 2 cut(s) 22, 921
BamHI GGATCC 1 cut(s) 1576
BanI GGYRCC 3 cut(s) 1500, 1728, 1998
BbsI GAAGAC 1 cut(s) 2067
Bbv12I GWGCWC 2 cut(s) 66, 1808
BbvI GCAGC 3 cut(s) 493, 1084, 1996
BccI CCATC 6 cut(s) 107, 553, 930, 1408, 1519, 2323
BceAI ACGGC 2 cut(s) 1380, 1488
BciT130I CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
BciVI GTATCC 1 cut(s) 1051
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 93, 2327
BcuI ACTAGT 1 cut(s) 2323
BfaI CTAG 3 cut(s) 1353, 2246, 2324
BfmI CTRYAG 3 cut(s) 410, 1516, 1530
BfoI RGCGCY 2 cut(s) 1421, 2406
BfrI CTTAAG 1 cut(s) 2338
BfuAI ACCTGC 1 cut(s) 1526
BfuI GTATCC 1 cut(s) 1051
BglII AGATCT 3 cut(s) 139, 1867, 2106
BisI GCNGC 4 cut(s) 180, 482, 1073, 1985
BlpI GCTNAGC 3 cut(s) 459, 989, 2197
BlsI GCNGC 4 cut(s) 181, 483, 1074, 1986
Bme1390I CCNGG 7 cut(s) 95, 488, 634, 1118, 1233, 1597, 2057
Bme18I GGWCC 1 cut(s) 2038
BmgT120I GGNCC 5 cut(s) 96, 671, 1107, 1484, 2038
BmiI GGNNCC 9 cut(s) 48, 941, 1108, 1502, 1578, 1594, 1730, 2000, 2040
BmrFI CCNGG 7 cut(s) 95, 488, 634, 1118, 1233, 1597, 2057
BmrI ACTGGG 2 cut(s) 2040, 2380
BmsI GCATC 4 cut(s) 868, 1055, 1513, 1941
BmuI ACTGGG 2 cut(s) 2040, 2380
BpiI GAAGAC 1 cut(s) 2067
Bpu1102I GCTNAGC 3 cut(s) 459, 989, 2197
Bpu14I TTCGAA 1 cut(s) 1863
BpuEI CTTGAG 1 cut(s) 2380
BpuMI CCSGG 1 cut(s) 95
BsaBI GATNNNNATC 4 cut(s) 291, 297, 1640, 1827
BsaI GGTCTC 1 cut(s) 2327
BsaJI CCNNGG 7 cut(s) 109, 118, 486, 487, 633, 1946, 2002
BsaWI WCCGGW 1 cut(s) 894
BsaXI ACNNNNNCTCC 4 cut(s) 687, 717, 1331, 1361
Bsc4I CCNNNNNNNGG 2 cut(s) 692, 1218
Bse1I ACTGG 5 cut(s) 63, 1813, 2035, 2217, 2375
Bse3DI GCAATG 2 cut(s) 941, 1067
Bse8I GATNNNNATC 4 cut(s) 291, 297, 1640, 1827
BseBI CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
BseDI CCNNGG 7 cut(s) 109, 118, 486, 487, 633, 1946, 2002
BseGI GGATG 4 cut(s) 1511, 1528, 1956, 2458
BseJI GATNNNNATC 4 cut(s) 291, 297, 1640, 1827
BseLI CCNNNNNNNGG 2 cut(s) 692, 1218
BseMI GCAATG 2 cut(s) 941, 1067
BseMII CTCAG 7 cut(s) 197, 331, 473, 1003, 1757, 1844, 2206
BseNI ACTGG 5 cut(s) 63, 1813, 2035, 2217, 2375
BseRI GAGGAG 2 cut(s) 1775, 2006
BseSI GKGCMC 3 cut(s) 66, 1808, 2191
BseXI GCAGC 3 cut(s) 493, 1084, 1996
BsgI GTGCAG 1 cut(s) 500
Bsh1236I CGCG 1 cut(s) 2018
Bsh1285I CGRYCG 2 cut(s) 44, 81
BshFI GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
BshNI GGYRCC 3 cut(s) 1500, 1728, 1998
BsiEI CGRYCG 2 cut(s) 44, 81
BsiHKAI GWGCWC 2 cut(s) 66, 1808
BsiSI CCGG 2 cut(s) 94, 895
BslFI GGGAC 2 cut(s) 1382, 1451
BslI CCNNNNNNNGG 2 cut(s) 692, 1218
BsmAI GTCTC 2 cut(s) 93, 2327
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 1382, 1451
BsmI GAATGC 1 cut(s) 2345
BsnI GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
Bso31I GGTCTC 1 cut(s) 2327
Bsp119I TTCGAA 1 cut(s) 1863
Bsp1286I GDGCHC 3 cut(s) 66, 1808, 2191
Bsp1407I TGTACA 1 cut(s) 2113
Bsp1720I GCTNAGC 3 cut(s) 459, 989, 2197
Bsp19I CCATGG 1 cut(s) 1946
BspACI CCGC 7 cut(s) 40, 149, 180, 423, 1044, 1709, 1929
BspANI GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
BspCNI CTCAG 7 cut(s) 196, 330, 472, 1002, 1756, 1845, 2205
BspFNI CGCG 1 cut(s) 2018
BspHI TCATGA 1 cut(s) 2083
BspLI GGNNCC 9 cut(s) 48, 941, 1108, 1502, 1578, 1594, 1730, 2000, 2040
BspMAI CTGCAG 2 cut(s) 414, 1520
BspMI ACCTGC 1 cut(s) 1526
BspPI GGATC 7 cut(s) 39, 492, 536, 677, 1051, 1571, 1584
BspT104I TTCGAA 1 cut(s) 1863
BspT107I GGYRCC 3 cut(s) 1500, 1728, 1998
BspTI CTTAAG 1 cut(s) 2338
BspTNI GGTCTC 1 cut(s) 2327
BsrBI CCGCTC 2 cut(s) 182, 1931
BsrDI GCAATG 2 cut(s) 941, 1067
BsrGI TGTACA 1 cut(s) 2113
BsrI ACTGG 5 cut(s) 63, 1813, 2035, 2217, 2375
BssECI CCNNGG 7 cut(s) 109, 118, 486, 487, 633, 1946, 2002
BssNAI GTATAC 1 cut(s) 1240
BssT1I CCWWGG 3 cut(s) 118, 1946, 2002
Bst1107I GTATAC 1 cut(s) 1240
Bst2UI CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
Bst4CI ACNGT 7 cut(s) 509, 791, 902, 1097, 1592, 1763, 2311
Bst6I CTCTTC 1 cut(s) 149
BstAFI CTTAAG 1 cut(s) 2338
BstAPI GCANNNNNTGC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 2113
BstBI TTCGAA 1 cut(s) 1863
BstC8I GCNNGC 5 cut(s) 421, 657, 1077, 1314, 2355
BstDEI CTNAG 9 cut(s) 183, 317, 459, 989, 1545, 1743, 1853, 2192, 2197
BstDSI CCRYGG 1 cut(s) 1946
BstENI CCTNNNNNAGG 1 cut(s) 690
BstF5I GGATG 4 cut(s) 1511, 1528, 1956, 2458
BstFNI CGCG 1 cut(s) 2018
BstH2I RGCGCY 2 cut(s) 1421, 2406
BstHHI GCGC 2 cut(s) 1420, 2405
BstMAI GTCTC 2 cut(s) 93, 2327
BstMCI CGRYCG 2 cut(s) 44, 81
BstMWI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 409, 559, 782, 1532, 1937, 1951
BstNI CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
BstNSI RCATGY 4 cut(s) 557, 988, 1849, 2357
BstSCI CCNGG 7 cut(s) 93, 486, 632, 1116, 1231, 1595, 2055
BstSFI CTRYAG 3 cut(s) 410, 1516, 1530
BstSLI GKGCMC 3 cut(s) 66, 1808, 2191
BstUI CGCG 1 cut(s) 2018
BstV1I GCAGC 3 cut(s) 493, 1084, 1996
BstV2I GAAGAC 1 cut(s) 2067
BstX2I RGATCY 8 cut(s) 31, 139, 497, 541, 1056, 1576, 1867, 2106
BstYI RGATCY 8 cut(s) 31, 139, 497, 541, 1056, 1576, 1867, 2106
BstZ17I GTATAC 1 cut(s) 1240
BsuI GTATCC 1 cut(s) 1051
BsuRI GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
BtgI CCRYGG 1 cut(s) 1946
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 4 cut(s) 1511, 1528, 1956, 2458
BtsI GCAGTG 1 cut(s) 2160
BtsIMutI CAGTG 6 cut(s) 325, 576, 1806, 2160, 2224, 2368
BveI ACCTGC 1 cut(s) 1526
Cac8I GCNNGC 5 cut(s) 421, 657, 1077, 1314, 2355
CaiI CAGNNNCTG 1 cut(s) 487
CciI TCATGA 1 cut(s) 2083
CfoI GCGC 2 cut(s) 1420, 2405
Cfr13I GGNCC 5 cut(s) 96, 671, 1107, 1484, 2038
CseI GACGC 1 cut(s) 1205
Csp6I GTAC 4 cut(s) 407, 1158, 1729, 2114
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviQI GTAC 4 cut(s) 407, 1158, 1729, 2114
DdeI CTNAG 9 cut(s) 183, 317, 459, 989, 1545, 1743, 1853, 2192, 2197
DraIII CACNNNGTG 1 cut(s) 2374
EaeI YGGCCR 3 cut(s) 20, 919, 1393
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco130I CCWWGG 3 cut(s) 118, 1946, 2002
Eco31I GGTCTC 1 cut(s) 2327
Eco47I GGWCC 1 cut(s) 2038
Eco57I CTGAAG 4 cut(s) 211, 671, 884, 1892
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 2038
EcoRI GAATTC 3 cut(s) 349, 1454, 2096
EcoRII CCWGG 6 cut(s) 486, 632, 1116, 1231, 1595, 2055
EcoT14I CCWWGG 3 cut(s) 118, 1946, 2002
ErhI CCWWGG 3 cut(s) 118, 1946, 2002
Esp3I CGTCTC 1 cut(s) 93
FalI AAGNNNNNCTT 2 cut(s) 1890, 1922
FaqI GGGAC 2 cut(s) 1382, 1451
FauI CCCGC 1 cut(s) 416
FauNDI CATATG 2 cut(s) 175, 1201
FblI GTMKAC 4 cut(s) 272, 869, 969, 1239
Fnu4HI GCNGC 4 cut(s) 180, 482, 1073, 1985
FokI GGATG 3 cut(s) 1498, 1535, 1963
Fsp4HI GCNGC 4 cut(s) 180, 482, 1073, 1985
FspBI CTAG 3 cut(s) 1353, 2246, 2324
GlaI GCGC 2 cut(s) 1419, 2404
GluI GCNGC 4 cut(s) 180, 482, 1073, 1985
HaeII RGCGCY 2 cut(s) 1421, 2406
HaeIII GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
HapII CCGG 2 cut(s) 94, 895
HgaI GACGC 1 cut(s) 1205
HhaI GCGC 2 cut(s) 1420, 2405
Hin6I GCGC 2 cut(s) 1418, 2403
HinP1I GCGC 2 cut(s) 1418, 2403
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 2 cut(s) 401, 560
HinfI GANTC 6 cut(s) 83, 157, 1136, 1666, 1855, 1902
HpaII CCGG 2 cut(s) 94, 895
HphI GGTGA 3 cut(s) 16, 503, 1886
Hpy166II GTNNAC 9 cut(s) 64, 273, 323, 870, 970, 1195, 1240, 1806, 2116
Hpy8I GTNNAC 9 cut(s) 64, 273, 323, 870, 970, 1195, 1240, 1806, 2116
HpyAV CCTTC 9 cut(s) 207, 238, 409, 694, 949, 953, 1019, 1497, 1916
HpyCH4III ACNGT 7 cut(s) 509, 791, 902, 1097, 1592, 1763, 2311
HpyCH4IV ACGT 4 cut(s) 220, 714, 1385, 2179
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 409, 559, 782, 1532, 1937, 1951
HpyF3I CTNAG 9 cut(s) 183, 317, 459, 989, 1545, 1743, 1853, 2192, 2197
HpySE526I ACGT 4 cut(s) 220, 714, 1385, 2179
HspAI GCGC 2 cut(s) 1418, 2403
KpnI GGTACC 1 cut(s) 1732
LmnI GCTCC 2 cut(s) 46, 2150
Lsp1109I GCAGC 3 cut(s) 493, 1084, 1996
LweI GCATC 4 cut(s) 868, 1055, 1513, 1941
MaeI CTAG 3 cut(s) 1353, 2246, 2324
MaeII ACGT 4 cut(s) 220, 714, 1385, 2179
MbiI CCGCTC 2 cut(s) 182, 1931
MboII GAAGA 8 cut(s) 166, 217, 253, 718, 1645, 1877, 2060, 2072
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 8 cut(s) 31, 139, 497, 541, 1056, 1576, 1867, 2106
MhlI GDGCHC 3 cut(s) 66, 1808, 2191
MlsI TGGCCA 2 cut(s) 22, 921
MluI ACGCGT 1 cut(s) 2016
MluNI TGGCCA 2 cut(s) 22, 921
MlyI GAGTC 2 cut(s) 166, 1864
MmeI TCCRAC 2 cut(s) 24, 2293
Mox20I TGGCCA 2 cut(s) 22, 921
MroXI GAANNNNTTC 1 cut(s) 853
MscI TGGCCA 2 cut(s) 22, 921
MseI TTAA 5 cut(s) 312, 980, 1617, 1682, 2339
MslI CAYNNNNRTG 3 cut(s) 927, 998, 1793
Msp20I TGGCCA 2 cut(s) 22, 921
MspCI CTTAAG 1 cut(s) 2338
MspI CCGG 2 cut(s) 94, 895
MspR9I CCNGG 7 cut(s) 95, 488, 634, 1118, 1233, 1597, 2057
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 2345
MvaI CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
MvnI CGCG 1 cut(s) 2018
MwoI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 409, 559, 782, 1532, 1937, 1951
NciI CCSGG 1 cut(s) 95
NcoI CCATGG 1 cut(s) 1946
NdeI CATATG 2 cut(s) 175, 1201
NlaIV GGNNCC 9 cut(s) 48, 941, 1108, 1502, 1578, 1594, 1730, 2000, 2040
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 6 cut(s) 4, 255, 1000, 1249, 1505, 1553
NspI RCATGY 4 cut(s) 557, 988, 1849, 2357
NspV TTCGAA 1 cut(s) 1863
PaeI GCATGC 1 cut(s) 2357
PagI TCATGA 1 cut(s) 2083
PasI CCCWGGG 1 cut(s) 487
PciI ACATGT 1 cut(s) 1845
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PctI GAATGC 1 cut(s) 2345
PdmI GAANNNNTTC 1 cut(s) 853
PfeI GAWTC 4 cut(s) 83, 1136, 1666, 1902
PkrI GCNGC 4 cut(s) 181, 483, 1074, 1986
Ple19I CGATCG 1 cut(s) 81
PleI GAGTC 2 cut(s) 165, 1863
PpsI GAGTC 2 cut(s) 165, 1863
PpuMI RGGWCCY 1 cut(s) 2038
PscI ACATGT 1 cut(s) 1845
PsiI TTATAA 1 cut(s) 2300
Psp5II RGGWCCY 1 cut(s) 2038
Psp6I CCWGG 6 cut(s) 486, 632, 1116, 1231, 1595, 2055
PspGI CCWGG 6 cut(s) 486, 632, 1116, 1231, 1595, 2055
PspN4I GGNNCC 9 cut(s) 48, 941, 1108, 1502, 1578, 1594, 1730, 2000, 2040
PspPI GGNCC 5 cut(s) 96, 671, 1107, 1484, 2038
PspPPI RGGWCCY 1 cut(s) 2038
PsrI GAACNNNNNNTAC 2 cut(s) 2378, 2410
PstI CTGCAG 2 cut(s) 414, 1520
PstNI CAGNNNCTG 1 cut(s) 487
PsuI RGATCY 8 cut(s) 31, 139, 497, 541, 1056, 1576, 1867, 2106
PvuI CGATCG 1 cut(s) 81
RsaI GTAC 4 cut(s) 408, 1159, 1730, 2115
RsaNI GTAC 4 cut(s) 407, 1158, 1729, 2114
RseI CAYNNNNRTG 3 cut(s) 927, 998, 1793
SaqAI TTAA 5 cut(s) 312, 980, 1617, 1682, 2339
SatI GCNGC 4 cut(s) 180, 482, 1073, 1985
Sau96I GGNCC 5 cut(s) 96, 671, 1107, 1484, 2038
SbfI CCTGCAGG 1 cut(s) 1520
SchI GAGTC 2 cut(s) 166, 1864
ScrFI CCNGG 7 cut(s) 95, 488, 634, 1118, 1233, 1597, 2057
SdaI CCTGCAGG 1 cut(s) 1520
SduI GDGCHC 3 cut(s) 66, 1808, 2191
SfaNI GCATC 4 cut(s) 868, 1055, 1513, 1941
SfcI CTRYAG 3 cut(s) 410, 1516, 1530
SfuI TTCGAA 1 cut(s) 1863
SinI GGWCC 1 cut(s) 2038
SmiMI CAYNNNNRTG 3 cut(s) 927, 998, 1793
SmlI CTYRAG 2 cut(s) 2338, 2359
SmoI CTYRAG 2 cut(s) 2338, 2359
SpeI ACTAGT 1 cut(s) 2323
SphI GCATGC 1 cut(s) 2357
Sse8387I CCTGCAGG 1 cut(s) 1520
SsiI CCGC 7 cut(s) 40, 149, 180, 423, 1044, 1709, 1929
SspI AATATT 2 cut(s) 1479, 1703
SspMI CTAG 3 cut(s) 1353, 2246, 2324
StyD4I CCNGG 7 cut(s) 93, 486, 632, 1116, 1231, 1595, 2055
StyI CCWWGG 3 cut(s) 118, 1946, 2002
TaaI ACNGT 7 cut(s) 509, 791, 902, 1097, 1592, 1763, 2311
TaiI ACGT 4 cut(s) 223, 717, 1388, 2182
TaqI TCGA 5 cut(s) 81, 211, 522, 548, 1863
TatI WGTACW 2 cut(s) 406, 2113
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 4 cut(s) 83, 1136, 1666, 1902
Tru1I TTAA 5 cut(s) 312, 980, 1617, 1682, 2339
Tru9I TTAA 5 cut(s) 312, 980, 1617, 1682, 2339
TscAI CASTG 6 cut(s) 325, 576, 1813, 2160, 2224, 2375
TseFI GTSAC 6 cut(s) 4, 255, 1000, 1249, 1505, 1553
TseI GCWGC 3 cut(s) 481, 1072, 1984
Tsp45I GTSAC 6 cut(s) 4, 255, 1000, 1249, 1505, 1553
TspGWI ACGGA 4 cut(s) 23, 150, 955, 1145
TspRI CASTG 6 cut(s) 325, 576, 1813, 2160, 2224, 2375
Vha464I CTTAAG 1 cut(s) 2338
VneI GTGCAC 2 cut(s) 62, 1804
VpaK11BI GGWCC 1 cut(s) 2038
XagI CCTNNNNNAGG 1 cut(s) 690
XapI RAATTY 7 cut(s) 349, 756, 1166, 1454, 2090, 2096, 2414
XceI RCATGY 4 cut(s) 557, 988, 1849, 2357
XcmI CCANNNNNNNNNTGG 1 cut(s) 1667
XmiI GTMKAC 4 cut(s) 272, 869, 969, 1239
XmnI GAANNNNTTC 1 cut(s) 853
XspI CTAG 3 cut(s) 1353, 2246, 2324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.