Rh6AG148400

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003618
Physical Location & Seq
Reverse (-)
23353555 .. 23364286
10732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG148400.1

Sequence Viewer

Length: 1935 bp
ATGGGAGGATGCTGTCCCCCGATGGATCTGTTCAGGTCGGAGCCTATGCAGCTCGTCCAGATCATCATCCCGATCGAGTCCGCCCACCTCACCGTCTCTTATCTCGGCGACCTCGGCCTCCTCCAATTCAAAGACCTTAATTCAGAGAAGAGCCCGTTTCAGAGGACTTATGCTACGCAGATTAAAAGAGCTGGTGAGATGGCACGCAAATTACGTTTCTTCAAGGACCAAATGTTAAAGGCTGGTCTGCCTCCTACAAAGTCTAGAAGACAAGCTGACCTCAATGTAGATGATCTAGAGGTAAAACTTGGTGAGCTCGAGGCTGAGCTGATTGAAATTAATGCGAATGGTGAAAAATTGCAGCGTTCATATAATGAACTCTTAGAATATAAGCTTGTCCTGCAAAAGGCTGGTGAGTTTTTTCATTCAGCTGAAAGCAGTGCCAGGTCGCAGCAGAGAGATGAATCCCGTCATACTGGTGATGATGCCCTGGATACACCATTATTGGTGGACCAAGAATCATCAACTGATCCATCAAAGCAAGTTAAGTTGGGGTTTCTCACCGGTCTTGTTCCTAGGGGAAAGTCTTTGGCATTTGAGAGGATTCTATTTCGAGCTACTAGGGGTAATGTTTTTCTAAGGCAGGCCGTTGTTGAGAATCCTGTTACAGATCCAGTGACAGGAGAGAAGATTGAGAAAAATGTTTTTGTTGTATTCTATTCTGGAGAAAGAGCAAAGAACAAGATTCTTAAAATTTGTGATGCCTTTGGAGCCAATCGTTACCCTTTTACTGAGGACCTAAGTAAACAAGCTCAAACAATTAATGAGGTTACAGGTAAACTTTCAGAGCTAAAGACTACTATAGATATTGGAGTCTCACACCGGGAAAGTTTGTTACAGACTATTGGCGAACATTATGAGCAATGGAACCATTTGGCGAGGAAGGAGAAAGCCATACACCACACTCTTAATATGCTTAGCCTCGATGTAACCAAAAAATGTCTTGTGGCTGAGGGGTGGAGTCCTATTTTTGCATCGAAGCAGATCCAGGAGGCATTGCAGCGGGCAGCTTTTGACTCCAATTCTCAAGTTGGAGCTATCTTCCAGGTTCTGCATACAAAGGAAGCACCACCAACATATTTCCGCACAAACAAGTTCACGTCTTCATTTCAAGAGATTGTTGATGCATATGGTGTGGCCAAGTATCAAGAAGCAAATCCTGCTGTATATACTATCATCACATTTCCATTTCTTTTTGCTGTCATGTTTGGTGATTGGGGACATGGACTATGTTTGCTTCTTGCGACATTATATTTGATAGTCAGGGAAAGGAAATTTTCTAATGAGAAACTTGGAGATATCATGGAGATGGCATTTGGTGGCCGTTATGTTATTCTATTGATGTCAATTTTCTCAATCTACACTGGTTTCATCTATAATGAGTTCTTCTCTGTCCCATTTGAACTGTTTGGTCGCTCAGCATATGCATGCCGTGATCTTTCTTGCAGAGATGCCACTACTGCTGGCTTGATTAAGGTGGGTCCCACATACCCATTTGGTCTGGATCCTGTGTGGCATGGATCTCGCAGTGAGCTGCCATTCTTAAACTCATTGAAGATGAAAATGTCAATTCTTATTGGGGTGGTCCAAATGAACCTTGGAATTGTGATAAGTTATTTCAATGCAAAGTTCTTTCAAAATGGTCTGAACGTTTGGTTCCAATTTGTCCCTCAGCTGATATTCTTAAACAGTCTATTTGGCTACTTGTCTGTTCTGATTGTTATGAAGTGGTGGACTGGTTCAAAAGTTGATCTATACCATGTAATGATATATATGTTCCTCAGTCCTACTGATGAGCTAGGTGAAAATCAGCTTTTCAGTGGTCAGAAAACAGTTCAGGTAGTCCCTCTTACCACTCTTGTTTATATGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

644

Amino Acids

72.92

Weight (kDa)

7.18

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 34 - 641 2.8e-220 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1933
AciI CCGC 3 cut(s) 81, 1063, 1144
AclI AACGTT 1 cut(s) 1710
AclWI GGATC 7 cut(s) 33, 524, 665, 1039, 1559, 1572, 1588
AcoI YGGCCR 2 cut(s) 1197, 1381
AcsI RAATTY 2 cut(s) 753, 1334
AfiI CCNNNNNNNGG 2 cut(s) 406, 680
AgeI ACCGGT 1 cut(s) 563
AgsI TTSAA 9 cut(s) 130, 223, 335, 1172, 1463, 1615, 1681, 1697, 1803
AjiI CACGTC 1 cut(s) 1161
AjnI CCWGG 4 cut(s) 443, 489, 1047, 1104
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 2 cut(s) 100, 880
AlwI GGATC 7 cut(s) 33, 524, 665, 1039, 1559, 1572, 1588
AlwNI CAGNNNCTG 1 cut(s) 1111
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 4 cut(s) 115, 645, 1197, 1381
ApeKI GCWGC 6 cut(s) 49, 361, 451, 1060, 1067, 1594
ApoI RAATTY 2 cut(s) 753, 1334
ArsI GACNNNNNNTTYG 2 cut(s) 1297, 1329
AseI ATTAAT 2 cut(s) 339, 822
AsiGI ACCGGT 1 cut(s) 563
AspA2I CCTAGG 1 cut(s) 575
AspS9I GGNCC 5 cut(s) 226, 511, 796, 1541, 1645
AsuC2I CCSGG 1 cut(s) 884
AsuHPI GGTGA 9 cut(s) 82, 206, 323, 362, 425, 491, 553, 1283, 1874
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 5 cut(s) 226, 511, 796, 1541, 1645
AvrII CCTAGG 1 cut(s) 575
BalI TGGCCA 1 cut(s) 1199
BamHI GGATCC 1 cut(s) 1564
BanII GRGCYC 2 cut(s) 155, 318
BbsI GAAGAC 2 cut(s) 274, 1155
Bbv12I GWGCWC 1 cut(s) 318
BbvCI CCTCAGC 2 cut(s) 1011, 1731
BbvI GCAGC 6 cut(s) 61, 373, 463, 1072, 1079, 1581
BccI CCATC 4 cut(s) 16, 193, 541, 1363
BceAI ACGGC 3 cut(s) 632, 1368, 1476
BcgI CGANNNNNNTGC 2 cut(s) 1565, 1599
BciT130I CCWGG 4 cut(s) 445, 491, 1049, 1106
BciVI GTATCC 1 cut(s) 487
BcnI CCSGG 1 cut(s) 884
BcoDI GTCTC 2 cut(s) 100, 880
BfaI CTAG 5 cut(s) 264, 296, 576, 621, 1859
BfmI CTRYAG 1 cut(s) 861
BfuI GTATCC 1 cut(s) 487
BisI GCNGC 6 cut(s) 50, 362, 452, 1061, 1068, 1595
BlnI CCTAGG 1 cut(s) 575
BlpI GCTNAGC 3 cut(s) 324, 977, 1477
BlsI GCNGC 6 cut(s) 51, 363, 453, 1062, 1069, 1596
Bme1390I CCNGG 5 cut(s) 445, 491, 884, 1049, 1106
Bme18I GGWCC 5 cut(s) 226, 511, 796, 1541, 1645
BmeT110I CYCGRG 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 1161
BmgT120I GGNCC 5 cut(s) 226, 511, 796, 1541, 1645
BmiI GGNNCC 7 cut(s) 42, 772, 929, 1542, 1543, 1566, 1718
BmrFI CCNGG 5 cut(s) 445, 491, 884, 1049, 1106
BmsI GCATC 5 cut(s) 475, 751, 1043, 1174, 1501
BpiI GAAGAC 2 cut(s) 274, 1155
BplI GAGNNNNNCTC 2 cut(s) 1433, 1465
BpmI CTGGAG 1 cut(s) 744
Bpu10I CCTNAGC 2 cut(s) 1011, 1731
Bpu1102I GCTNAGC 3 cut(s) 324, 977, 1477
BpuEI CTTGAG 1 cut(s) 1071
BpuMI CCSGG 1 cut(s) 884
BsaBI GATNNNNATC 1 cut(s) 65
BsaJI CCNNGG 4 cut(s) 112, 489, 575, 1657
BsaWI WCCGGW 1 cut(s) 563
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 680
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 4 cut(s) 481, 674, 1429, 1801
Bse3DI GCAATG 2 cut(s) 929, 1055
Bse8I GATNNNNATC 1 cut(s) 65
BseBI CCWGG 4 cut(s) 445, 491, 1049, 1106
BseDI CCNNGG 4 cut(s) 112, 489, 575, 1657
BseGI GGATG 2 cut(s) 14, 66
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 2 cut(s) 406, 680
BseMI GCAATG 2 cut(s) 929, 1055
BseMII CTCAG 6 cut(s) 315, 783, 1002, 1491, 1745, 1855
BseNI ACTGG 4 cut(s) 481, 674, 1429, 1801
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 6 cut(s) 61, 373, 463, 1072, 1079, 1581
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 4 cut(s) 117, 647, 1199, 1383
BshTI ACCGGT 1 cut(s) 563
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 564, 883
BslFI GGGAC 5 cut(s) 1293, 1439, 1527, 1712, 1889
BslI CCNNNNNNNGG 2 cut(s) 406, 680
BsmAI GTCTC 2 cut(s) 100, 880
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 5 cut(s) 1293, 1439, 1527, 1712, 1889
BsnI GGCC 4 cut(s) 117, 647, 1199, 1383
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 155, 318
Bsp1720I GCTNAGC 3 cut(s) 324, 977, 1477
BspACI CCGC 3 cut(s) 81, 1063, 1144
BspANI GGCC 4 cut(s) 117, 647, 1199, 1383
BspCNI CTCAG 6 cut(s) 316, 784, 1003, 1490, 1744, 1854
BspLI GGNNCC 7 cut(s) 42, 772, 929, 1542, 1543, 1566, 1718
BspPI GGATC 7 cut(s) 33, 524, 665, 1039, 1559, 1572, 1588
BspQI GCTCTTC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 929, 1055
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 4 cut(s) 481, 674, 1429, 1801
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 4 cut(s) 112, 489, 575, 1657
BssT1I CCWWGG 2 cut(s) 575, 1657
Bst2UI CCWGG 4 cut(s) 445, 491, 1049, 1106
Bst4CI ACNGT 4 cut(s) 94, 1467, 1751, 1894
Bst6I CTCTTC 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 1220
BstC8I GCNNGC 5 cut(s) 205, 645, 1065, 1489, 1525
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 2 cut(s) 14, 66
BstMAI GTCTC 2 cut(s) 100, 880
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 6 cut(s) 49, 114, 400, 770, 1220, 1520
BstNI CCWGG 4 cut(s) 445, 491, 1049, 1106
BstNSI RCATGY 1 cut(s) 1491
BstSCI CCNGG 5 cut(s) 443, 489, 882, 1047, 1104
BstSFI CTRYAG 1 cut(s) 861
BstV1I GCAGC 6 cut(s) 61, 373, 463, 1072, 1079, 1581
BstV2I GAAGAC 2 cut(s) 274, 1155
BstX2I RGATCY 5 cut(s) 25, 670, 1044, 1564, 1580
BstYI RGATCY 5 cut(s) 25, 670, 1044, 1564, 1580
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 4 cut(s) 117, 647, 1199, 1383
BtrI CACGTC 1 cut(s) 1161
BtsCI GGATG 2 cut(s) 14, 66
BtsI GCAGTG 2 cut(s) 445, 1594
BtsIMutI CAGTG 5 cut(s) 445, 681, 1422, 1594, 1885
Cac8I GCNNGC 5 cut(s) 205, 645, 1065, 1489, 1525
CaiI CAGNNNCTG 1 cut(s) 1111
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 5 cut(s) 226, 511, 796, 1541, 1645
CspAI ACCGGT 1 cut(s) 563
CviAII CATG 6 cut(s) 1264, 1283, 1363, 1488, 1577, 1820
EaeI YGGCCR 2 cut(s) 1197, 1381
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 2 cut(s) 575, 1657
Eco24I GRGCYC 2 cut(s) 155, 318
Eco32I GATATC 1 cut(s) 1360
Eco47I GGWCC 5 cut(s) 226, 511, 796, 1541, 1645
Eco53kI GAGCTC 1 cut(s) 316
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO109I RGGNCCY 2 cut(s) 796, 1541
EcoRII CCWGG 4 cut(s) 443, 489, 1047, 1104
EcoRV GATATC 1 cut(s) 1360
EcoT14I CCWWGG 2 cut(s) 575, 1657
EcoT22I ATGCAT 2 cut(s) 1189, 1489
EcoT38I GRGCYC 2 cut(s) 155, 318
ErhI CCWWGG 2 cut(s) 575, 1657
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 6 cut(s) 1267, 1286, 1366, 1491, 1580, 1823
FaqI GGGAC 5 cut(s) 1293, 1439, 1527, 1712, 1889
FatI CATG 6 cut(s) 1263, 1282, 1362, 1487, 1576, 1819
FauI CCCGC 1 cut(s) 1056
FauNDI CATATG 2 cut(s) 1189, 1483
Fnu4HI GCNGC 6 cut(s) 50, 362, 452, 1061, 1068, 1595
FokI GGATG 2 cut(s) 21, 53
FriOI GRGCYC 2 cut(s) 155, 318
Fsp4HI GCNGC 6 cut(s) 50, 362, 452, 1061, 1068, 1595
FspBI CTAG 5 cut(s) 264, 296, 576, 621, 1859
GluI GCNGC 6 cut(s) 50, 362, 452, 1061, 1068, 1595
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 4 cut(s) 117, 647, 1199, 1383
HapII CCGG 2 cut(s) 564, 883
Hin1II CATG 6 cut(s) 1267, 1286, 1366, 1491, 1580, 1823
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 9 cut(s) 77, 464, 518, 604, 658, 745, 873, 1021, 1076
HpaII CCGG 2 cut(s) 564, 883
HphI GGTGA 9 cut(s) 82, 206, 323, 362, 425, 491, 553, 1283, 1874
Hpy166II GTNNAC 5 cut(s) 511, 806, 839, 1158, 1794
Hpy188I TCNGA 7 cut(s) 40, 145, 162, 847, 1707, 1776, 1887
Hpy188III TCNNGA 8 cut(s) 58, 70, 264, 296, 723, 1172, 1208, 1562
Hpy8I GTNNAC 5 cut(s) 511, 806, 839, 1158, 1794
HpyAV CCTTC 1 cut(s) 937
HpyCH4III ACNGT 4 cut(s) 94, 1467, 1751, 1894
HpyCH4IV ACGT 3 cut(s) 214, 1160, 1710
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 114, 400, 770, 1220, 1520
HpySE526I ACGT 3 cut(s) 214, 1160, 1710
Hsp92II CATG 6 cut(s) 1267, 1286, 1366, 1491, 1580, 1823
KflI GGGWCCC 1 cut(s) 1541
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 3 cut(s) 40, 770, 1094
Lsp1109I GCAGC 6 cut(s) 61, 373, 463, 1072, 1079, 1581
LweI GCATC 5 cut(s) 475, 751, 1043, 1174, 1501
MaeI CTAG 5 cut(s) 264, 296, 576, 621, 1859
MaeII ACGT 3 cut(s) 214, 1160, 1710
MaeIII GTNAC 6 cut(s) 664, 676, 779, 829, 894, 988
MboII GAAGA 8 cut(s) 160, 211, 279, 700, 1093, 1155, 1438, 1627
MflI RGATCY 5 cut(s) 25, 670, 1044, 1564, 1580
MhlI GDGCHC 2 cut(s) 155, 318
MlsI TGGCCA 1 cut(s) 1199
MluNI TGGCCA 1 cut(s) 1199
MlyI GAGTC 4 cut(s) 86, 882, 1030, 1070
MmeI TCCRAC 2 cut(s) 18, 1072
Mox20I TGGCCA 1 cut(s) 1199
Mph1103I ATGCAT 2 cut(s) 1189, 1489
MscI TGGCCA 1 cut(s) 1199
MslI CAYNNNNRTG 3 cut(s) 477, 1367, 1486
Msp20I TGGCCA 1 cut(s) 1199
MspA1I CMGCKG 3 cut(s) 431, 1063, 1735
MspI CCGG 2 cut(s) 564, 883
MspR9I CCNGG 5 cut(s) 445, 491, 884, 1049, 1106
MvaI CCWGG 4 cut(s) 445, 491, 1049, 1106
MwoI GCNNNNNNNGC 6 cut(s) 49, 114, 400, 770, 1220, 1520
NciI CCSGG 1 cut(s) 884
NdeI CATATG 2 cut(s) 1189, 1483
NlaIII CATG 6 cut(s) 1267, 1286, 1366, 1491, 1580, 1823
NlaIV GGNNCC 7 cut(s) 42, 772, 929, 1542, 1543, 1566, 1718
NmeAIII GCCGAG 2 cut(s) 84, 93
NmuCI GTSAC 1 cut(s) 676
NsiI ATGCAT 2 cut(s) 1189, 1489
NspI RCATGY 1 cut(s) 1491
PaeI GCATGC 1 cut(s) 1491
PaeR7I CTCGAG 1 cut(s) 317
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PfeI GAWTC 5 cut(s) 464, 518, 604, 658, 745
PfoI TCCNGGA 1 cut(s) 1047
PinAI ACCGGT 1 cut(s) 563
PkrI GCNGC 6 cut(s) 51, 363, 453, 1062, 1069, 1596
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 4 cut(s) 85, 881, 1029, 1070
PpsI GAGTC 4 cut(s) 85, 881, 1029, 1070
PpuMI RGGWCCY 2 cut(s) 796, 1541
PshBI ATTAAT 2 cut(s) 339, 822
PsiI TTATAA 1 cut(s) 1933
Psp124BI GAGCTC 1 cut(s) 318
Psp1406I AACGTT 1 cut(s) 1710
Psp5II RGGWCCY 2 cut(s) 796, 1541
Psp6I CCWGG 4 cut(s) 443, 489, 1047, 1104
PspGI CCWGG 4 cut(s) 443, 489, 1047, 1104
PspN4I GGNNCC 7 cut(s) 42, 772, 929, 1542, 1543, 1566, 1718
PspPI GGNCC 5 cut(s) 226, 511, 796, 1541, 1645
PspPPI RGGWCCY 2 cut(s) 796, 1541
PspXI VCTCGAGB 1 cut(s) 317
PstNI CAGNNNCTG 1 cut(s) 1111
PsuI RGATCY 5 cut(s) 25, 670, 1044, 1564, 1580
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 431, 1735
RseI CAYNNNNRTG 3 cut(s) 477, 1367, 1486
SacI GAGCTC 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 143
SatI GCNGC 6 cut(s) 50, 362, 452, 1061, 1068, 1595
Sau96I GGNCC 5 cut(s) 226, 511, 796, 1541, 1645
SchI GAGTC 4 cut(s) 86, 882, 1030, 1070
ScrFI CCNGG 5 cut(s) 445, 491, 884, 1049, 1106
SduI GDGCHC 2 cut(s) 155, 318
SfaNI GCATC 5 cut(s) 475, 751, 1043, 1174, 1501
SfcI CTRYAG 1 cut(s) 861
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 5 cut(s) 226, 511, 796, 1541, 1645
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 3 cut(s) 477, 1367, 1486
SmlI CTYRAG 2 cut(s) 317, 1086
SmoI CTYRAG 2 cut(s) 317, 1086
SphI GCATGC 1 cut(s) 1491
SsiI CCGC 3 cut(s) 81, 1063, 1144
SspMI CTAG 5 cut(s) 264, 296, 576, 621, 1859
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 5 cut(s) 443, 489, 882, 1047, 1104
StyI CCWWGG 2 cut(s) 575, 1657
TaaI ACNGT 4 cut(s) 94, 1467, 1751, 1894
TaiI ACGT 3 cut(s) 217, 1163, 1713
TaqI TCGA 5 cut(s) 75, 318, 613, 984, 1037
TfiI GAWTC 5 cut(s) 464, 518, 604, 658, 745
TscAI CASTG 5 cut(s) 445, 681, 1429, 1594, 1885
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 6 cut(s) 49, 361, 451, 1060, 1067, 1594
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 9 cut(s) 357, 390, 413, 477, 1155, 1420, 1634, 1667, 1799
TspRI CASTG 5 cut(s) 445, 681, 1429, 1594, 1885
VpaK11BI GGWCC 5 cut(s) 226, 511, 796, 1541, 1645
VspI ATTAAT 2 cut(s) 339, 822
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 2 cut(s) 753, 1334
XbaI TCTAGA 2 cut(s) 263, 295
XceI RCATGY 1 cut(s) 1491
XcmI CCANNNNNNNNNTGG 1 cut(s) 1655
XhoI CTCGAG 1 cut(s) 317
XmaJI CCTAGG 1 cut(s) 575
XspI CTAG 5 cut(s) 264, 296, 576, 621, 1859
Zsp2I ATGCAT 2 cut(s) 1189, 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.