AT2G22370

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002479
Physical Location & Seq
Reverse (-)
9500784 .. 9502210
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G22370.1

Sequence Viewer

Length: 660 bp
ATGTCAATGGAGTGTGTGGTTCAAGGAATCATCGAGACACAGCATGTTGAAGCTCTTGAGATTCTTCTTCAAGGTCTTTGTGGTGTTCAACGCGAACGTTTAAGAGTTCATGAGTTATGCTTAAGAAGCGGCCCAAATCTTGGAGTTGTGTCATCAGAAGTTAGGCTATTATGTGATCTTGATCAACCCGAACCCACATGGACGGTTAAACATGTTGGTGGTGCGATGAGAGGTGCAGGAGCTGATCAAATCTCTGTTTTGGTTAGAAATATGATTGAAAGTAAAGTAAGCAAGAATGCTTTGCGTATGTTCTATGCACTCGGTTACAAACTAGACCATGAGCTTCTCAAAGTCGGATTCGCCTTTCATTTCCAACGGACTGCGCACATAAGTGTTTCTGTATCTTCTGTTAATAAGATGCCTAAAGTTCATGCTATAGATGAGGCTGTTCCTGTAACACCTGGTATGCAGATTGTTGATGTAACTGCTCCTGCAACTTCTGAGAATTACAGTGAAGTCGCTGCTGCTGTTTCATCTTTCTGTGAATTTCTTGCTCCGCTTGTTCACTTGTCGAAACCGTCTATCTCCACGGGGGTTGTGCCAACCGCTGCTGCGGCGGCTGCATCTCTTATGTCGGATGGAGGAGGTACTACATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000414 GO:0000428 GO:0001101 GO:0002831 GO:0003002 GO:0003006 GO:0003674 GO:0003712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006353 GO:0006355 GO:0006366 GO:0006369 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010219 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0023051 GO:0030880 GO:0031047 GO:0031050 GO:0031056 GO:0031060 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031399 GO:0031554 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032991 GO:0033043 GO:0033044 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043244 GO:0043254 GO:0043331 GO:0043900 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048441 GO:0048442 GO:0048443 GO:0048464 GO:0048465 GO:0048466 GO:0048467 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061458 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090567 GO:0090575 GO:0097305 GO:0097659 GO:0099402 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000113 GO:2000142 GO:2000241 GO:2001023 GO:2001038 GO:2001141 GO:2001253
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

23.36

Weight (kDa)

6.05

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 384
AccB7I CCANNNNNTGG 1 cut(s) 140
AccII CGCG 1 cut(s) 93
AciI CCGC 5 cut(s) 129, 557, 606, 614, 617
AclI AACGTT 1 cut(s) 97
AcsI RAATTY 1 cut(s) 545
AfaI GTAC 1 cut(s) 649
AfiI CCNNNNNNNGG 1 cut(s) 140
AflII CTTAAG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 211
AgsI TTSAA 5 cut(s) 23, 50, 71, 89, 278
AjnI CCWGG 1 cut(s) 460
AleI CACNNNNGTG 1 cut(s) 390
AluBI AGCT 3 cut(s) 53, 242, 343
AluI AGCT 3 cut(s) 53, 242, 343
Alw26I GTCTC 1 cut(s) 29
AlwNI CAGNNNCTG 1 cut(s) 242
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 5 cut(s) 521, 524, 608, 611, 620
ApoI RAATTY 1 cut(s) 545
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 1 cut(s) 131
BbvI GCAGC 5 cut(s) 508, 511, 595, 598, 607
BccI CCATC 1 cut(s) 632
BciT130I CCWGG 1 cut(s) 462
BclI TGATCA 2 cut(s) 181, 244
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 435
BfrI CTTAAG 1 cut(s) 121
BisI GCNGC 8 cut(s) 130, 522, 525, 609, 612, 615, 618, 621
BlsI GCNGC 8 cut(s) 131, 523, 526, 610, 613, 616, 619, 622
Bme1390I CCNGG 1 cut(s) 462
BmgT120I GGNCC 1 cut(s) 131
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 408, 632
BpuEI CTTGAG 1 cut(s) 77
BsaBI GATNNNNATC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 588
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse8I GATNNNNATC 1 cut(s) 180
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 1 cut(s) 643
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 1 cut(s) 140
BseMII CTCAG 1 cut(s) 492
BseRI GAGGAG 1 cut(s) 657
BseXI GCAGC 5 cut(s) 508, 511, 595, 598, 607
BsgI GTGCAG 1 cut(s) 255
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 1 cut(s) 132
Bsp143I GATC 3 cut(s) 175, 181, 244
BspACI CCGC 5 cut(s) 129, 557, 606, 614, 617
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 109
BspTI CTTAAG 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 3 cut(s) 175, 181, 244
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 3 cut(s) 205, 512, 579
BstAFI CTTAAG 1 cut(s) 121
BstDEI CTNAG 1 cut(s) 501
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 1 cut(s) 643
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 3 cut(s) 178, 184, 247
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 3 cut(s) 175, 181, 244
BstMWI GCNNNNNNNGC 4 cut(s) 126, 614, 617, 620
BstNI CCWGG 1 cut(s) 462
BstNSI RCATGY 2 cut(s) 47, 215
BstSCI CCNGG 1 cut(s) 460
BstSFI CTRYAG 1 cut(s) 435
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 5 cut(s) 508, 511, 595, 598, 607
BsuRI GGCC 1 cut(s) 132
BtgI CCRYGG 1 cut(s) 588
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 1 cut(s) 643
BtsIMutI CAGTG 1 cut(s) 517
CaiI CAGNNNCTG 1 cut(s) 242
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 1 cut(s) 131
CsiI ACCWGGT 1 cut(s) 460
Csp6I GTAC 1 cut(s) 648
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 6 cut(s) 44, 110, 198, 212, 338, 431
CviJI RGCY 7 cut(s) 53, 132, 166, 242, 343, 446, 620
CviKI_1 RGCY 7 cut(s) 53, 132, 166, 242, 343, 446, 620
CviQI GTAC 1 cut(s) 648
DdeI CTNAG 1 cut(s) 501
DpnI GATC 3 cut(s) 177, 183, 246
DpnII GATC 3 cut(s) 175, 181, 244
EcoRII CCWGG 1 cut(s) 460
FaeI CATG 6 cut(s) 47, 113, 201, 215, 341, 434
FatI CATG 6 cut(s) 43, 109, 197, 211, 337, 430
FbaI TGATCA 2 cut(s) 181, 244
Fnu4HI GCNGC 8 cut(s) 130, 522, 525, 609, 612, 615, 618, 621
FokI GGATG 1 cut(s) 650
Fsp4HI GCNGC 8 cut(s) 130, 522, 525, 609, 612, 615, 618, 621
FspBI CTAG 1 cut(s) 332
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 1 cut(s) 384
GluI GCNGC 8 cut(s) 130, 522, 525, 609, 612, 615, 618, 621
HaeIII GGCC 1 cut(s) 132
HhaI GCGC 1 cut(s) 385
Hin1II CATG 6 cut(s) 47, 113, 201, 215, 341, 434
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HinfI GANTC 3 cut(s) 27, 61, 357
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 4 cut(s) 157, 356, 502, 637
Hpy188III TCNNGA 4 cut(s) 34, 56, 110, 179
Hpy8I GTNNAC 1 cut(s) 565
HpyCH4III ACNGT 3 cut(s) 205, 512, 579
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 5 cut(s) 236, 317, 469, 494, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 614, 617, 620
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 6 cut(s) 47, 113, 201, 215, 341, 434
HspAI GCGC 1 cut(s) 383
Ksp22I TGATCA 2 cut(s) 181, 244
Kzo9I GATC 3 cut(s) 175, 181, 244
LmnI GCTCC 3 cut(s) 239, 493, 559
LpnPI CCDG 5 cut(s) 222, 447, 465, 474, 504
Lsp1109I GCAGC 5 cut(s) 508, 511, 595, 598, 607
LweI GCATC 2 cut(s) 408, 632
MabI ACCWGGT 1 cut(s) 460
MaeI CTAG 1 cut(s) 332
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 3 cut(s) 323, 454, 481
MalI GATC 3 cut(s) 177, 183, 246
MboI GATC 3 cut(s) 175, 181, 244
MboII GAAGA 3 cut(s) 56, 59, 396
MluCI AATT 2 cut(s) 505, 545
MmeI TCCRAC 3 cut(s) 334, 397, 615
MnlI CCTC 4 cut(s) 224, 436, 635, 638
MseI TTAA 4 cut(s) 101, 122, 207, 411
MslI CAYNNNNRTG 2 cut(s) 216, 390
MspA1I CMGCKG 1 cut(s) 608
MspCI CTTAAG 1 cut(s) 121
MspR9I CCNGG 1 cut(s) 462
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 1 cut(s) 462
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 4 cut(s) 126, 614, 617, 620
NdeII GATC 3 cut(s) 175, 181, 244
NlaIII CATG 6 cut(s) 47, 113, 201, 215, 341, 434
NsbI TGCGCA 1 cut(s) 384
NspI RCATGY 2 cut(s) 47, 215
OliI CACNNNNGTG 1 cut(s) 390
PagI TCATGA 1 cut(s) 109
PciI ACATGT 1 cut(s) 211
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 3 cut(s) 27, 61, 357
PflMI CCANNNNNTGG 1 cut(s) 140
PkrI GCNGC 8 cut(s) 131, 523, 526, 610, 613, 616, 619, 622
PscI ACATGT 1 cut(s) 211
Psp1406I AACGTT 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspPI GGNCC 1 cut(s) 131
PstNI CAGNNNCTG 1 cut(s) 242
RsaI GTAC 1 cut(s) 649
RsaNI GTAC 1 cut(s) 648
RseI CAYNNNNRTG 2 cut(s) 216, 390
SaqAI TTAA 4 cut(s) 101, 122, 207, 411
SatI GCNGC 8 cut(s) 130, 522, 525, 609, 612, 615, 618, 621
Sau3AI GATC 3 cut(s) 175, 181, 244
Sau96I GGNCC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 462
SetI ASST 8 cut(s) 55, 76, 100, 235, 244, 345, 463, 649
SexAI ACCWGGT 1 cut(s) 460
SfaNI GCATC 2 cut(s) 408, 632
SfcI CTRYAG 1 cut(s) 435
SmiMI CAYNNNNRTG 2 cut(s) 216, 390
SmlI CTYRAG 2 cut(s) 56, 121
SmoI CTYRAG 2 cut(s) 56, 121
Sse9I AATT 2 cut(s) 505, 545
SsiI CCGC 5 cut(s) 129, 557, 606, 614, 617
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 460
TaaI ACNGT 3 cut(s) 205, 512, 579
TaiI ACGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 33, 572
TasI AATT 2 cut(s) 505, 545
TauI GCSGC 3 cut(s) 132, 617, 620
TfiI GAWTC 3 cut(s) 27, 61, 357
Tru1I TTAA 4 cut(s) 101, 122, 207, 411
Tru9I TTAA 4 cut(s) 101, 122, 207, 411
TscAI CASTG 1 cut(s) 517
TseI GCWGC 5 cut(s) 521, 524, 608, 611, 620
TspDTI ATGAA 4 cut(s) 98, 356, 419, 522
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 1 cut(s) 517
Van91I CCANNNNNTGG 1 cut(s) 140
Vha464I CTTAAG 1 cut(s) 121
XapI RAATTY 1 cut(s) 545
XceI RCATGY 2 cut(s) 47, 215
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.