RchiOBHm_Chr7g0238911

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002479
Physical Location & Seq
Forward (+)
64396780 .. 64397745
966 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21408

Sequence Viewer

Length: 492 bp
ATGATTTTCCTTTATCTAGGGACTGTACCTTCTGAGGTTCGACTTATTTGCAATCTTGAAGAAACTAAACCTACATGGACTGTGAGGTTCGTGGGGGGTGCGATGAGAGGTGCAAGTGCTGAGCAACTCTCAGTTTTAGTAAGAACTGTGATCGAAAGCAAAGCAAGCAAGAATGTGCTTCGCTTATTTTATGCACTTGGCTACAAGTTGGATCATGAGTTACTGAGAGTCGGTTTTGCGTTCCATTTCCAGAGAGGCACGCAGATGACTGTGACTGTGACTGTGTCATTAGTTAATAAGATGCCACAACTACACAATATAGATGAAGCTGTCCCTGTAACTCCCGGGATACAGTTGGTTGAAGTTACAGCCTCTGCATCATTTGAAACTTATACTGAAGTTGCGTCGACAGGAATTTCAACTGGGGTTGTTCCTATTGCTGCTGCCGCTGCTGCATCTCTCATGTCTGATGGTGGGGGTACTACCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000414 GO:0000428 GO:0001101 GO:0002831 GO:0003002 GO:0003006 GO:0003674 GO:0003712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006353 GO:0006355 GO:0006366 GO:0006369 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010219 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0023051 GO:0030880 GO:0031047 GO:0031050 GO:0031056 GO:0031060 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031399 GO:0031554 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032991 GO:0033043 GO:0033044 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043244 GO:0043254 GO:0043331 GO:0043900 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048441 GO:0048442 GO:0048443 GO:0048464 GO:0048465 GO:0048466 GO:0048467 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061458 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090567 GO:0090575 GO:0097305 GO:0097659 GO:0099402 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000113 GO:2000142 GO:2000241 GO:2001023 GO:2001038 GO:2001141 GO:2001253
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

17.45

Weight (kDa)

6.28

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 407
AciI CCGC 1 cut(s) 447
AclWI GGATC 1 cut(s) 219
AcsI RAATTY 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 2 cut(s) 27, 481
AgsI TTSAA 4 cut(s) 59, 362, 386, 420
AluBI AGCT 1 cut(s) 329
AluI AGCT 1 cut(s) 329
AlwI GGATC 1 cut(s) 219
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 344
ApeKI GCWGC 4 cut(s) 440, 443, 449, 452
ApoI RAATTY 1 cut(s) 414
AsuC2I CCSGG 2 cut(s) 345, 346
AvaI CYCGRG 1 cut(s) 344
BbvI GCAGC 4 cut(s) 427, 430, 436, 439
BccI CCATC 1 cut(s) 464
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BciVI GTATCC 1 cut(s) 342
BcnI CCSGG 2 cut(s) 345, 346
BfaI CTAG 1 cut(s) 17
BfuI GTATCC 1 cut(s) 342
BisI GCNGC 5 cut(s) 441, 444, 447, 450, 453
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 5 cut(s) 442, 445, 448, 451, 454
Bme1390I CCNGG 2 cut(s) 345, 346
BmeT110I CYCGRG 1 cut(s) 344
BmrFI CCNGG 2 cut(s) 345, 346
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 3 cut(s) 291, 386, 464
BmuI ACTGGG 1 cut(s) 432
BplI GAGNNNNNCTC 2 cut(s) 113, 145
Bpu1102I GCTNAGC 1 cut(s) 120
BpuMI CCSGG 2 cut(s) 345, 346
BsaJI CCNNGG 1 cut(s) 344
Bse1I ACTGG 1 cut(s) 427
BseDI CCNNGG 1 cut(s) 344
BseMII CTCAG 4 cut(s) 24, 111, 144, 215
BseNI ACTGG 1 cut(s) 427
BseXI GCAGC 4 cut(s) 427, 430, 436, 439
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 2 cut(s) 34, 317
BsmFI GGGAC 2 cut(s) 34, 317
BsoBI CYCGRG 1 cut(s) 344
Bsp143I GATC 2 cut(s) 150, 211
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 447
BspCNI CTCAG 4 cut(s) 25, 112, 143, 216
BspHI TCATGA 1 cut(s) 214
BspPI GGATC 1 cut(s) 219
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 2 cut(s) 150, 211
Bst4CI ACNGT 7 cut(s) 25, 82, 148, 271, 277, 283, 354
BstC8I GCNNGC 2 cut(s) 166, 260
BstDEI CTNAG 4 cut(s) 33, 120, 130, 224
BstKTI GATC 2 cut(s) 153, 214
BstMBI GATC 2 cut(s) 150, 211
BstMWI GCNNNNNNNGC 4 cut(s) 165, 446, 449, 452
BstSCI CCNGG 2 cut(s) 343, 344
BstV1I GCAGC 4 cut(s) 427, 430, 436, 439
BsuI GTATCC 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 116
Cac8I GCNNGC 2 cut(s) 166, 260
CaiI CAGNNNCTG 1 cut(s) 374
CciI TCATGA 1 cut(s) 214
Cfr9I CCCGGG 1 cut(s) 344
CseI GACGC 1 cut(s) 393
Csp6I GTAC 2 cut(s) 26, 480
CviAII CATG 3 cut(s) 75, 215, 463
CviJI RGCY 3 cut(s) 201, 329, 371
CviKI_1 RGCY 3 cut(s) 201, 329, 371
CviQI GTAC 2 cut(s) 26, 480
DdeI CTNAG 4 cut(s) 33, 120, 130, 224
DpnI GATC 2 cut(s) 152, 213
DpnII GATC 2 cut(s) 150, 211
Eco57I CTGAAG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 344
FaeI CATG 3 cut(s) 78, 218, 466
FaiI YATR 7 cut(s) 76, 192, 216, 320, 393, 464, 490
FaqI GGGAC 2 cut(s) 34, 317
FatI CATG 3 cut(s) 74, 214, 462
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 5 cut(s) 441, 444, 447, 450, 453
Fsp4HI GCNGC 5 cut(s) 441, 444, 447, 450, 453
FspBI CTAG 1 cut(s) 17
GluI GCNGC 5 cut(s) 441, 444, 447, 450, 453
HapII CCGG 1 cut(s) 345
HgaI GACGC 1 cut(s) 393
Hin1II CATG 3 cut(s) 78, 218, 466
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 1 cut(s) 228
HpaII CCGG 1 cut(s) 345
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 2 cut(s) 34, 469
Hpy188III TCNNGA 3 cut(s) 56, 215, 250
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 7 cut(s) 25, 82, 148, 271, 277, 283, 354
HpyCH4V TGCA 5 cut(s) 51, 113, 194, 377, 455
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 446, 449, 452
HpyF3I CTNAG 4 cut(s) 33, 120, 130, 224
Hsp92II CATG 3 cut(s) 78, 218, 466
Kzo9I GATC 2 cut(s) 150, 211
LpnPI CCDG 5 cut(s) 263, 348, 358, 396, 408
Lsp1109I GCAGC 4 cut(s) 427, 430, 436, 439
LweI GCATC 3 cut(s) 291, 386, 464
MaeI CTAG 1 cut(s) 17
MaeIII GTNAC 5 cut(s) 219, 271, 277, 337, 364
MalI GATC 2 cut(s) 152, 213
MboI GATC 2 cut(s) 150, 211
MboII GAAGA 1 cut(s) 71
MluCI AATT 1 cut(s) 414
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 5 cut(s) 28, 78, 101, 248, 382
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 345, 346
MwoI GCNNNNNNNGC 4 cut(s) 165, 446, 449, 452
NciI CCSGG 2 cut(s) 345, 346
NdeII GATC 2 cut(s) 150, 211
NlaIII CATG 3 cut(s) 78, 218, 466
NmuCI GTSAC 2 cut(s) 271, 277
PagI TCATGA 1 cut(s) 214
PflFI GACNNNGTC 1 cut(s) 283
PkrI GCNGC 5 cut(s) 442, 445, 448, 451, 454
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PstNI CAGNNNCTG 1 cut(s) 374
PsyI GACNNNGTC 1 cut(s) 283
RsaI GTAC 2 cut(s) 27, 481
RsaNI GTAC 2 cut(s) 26, 480
RseI CAYNNNNRTG 1 cut(s) 263
SalI GTCGAC 1 cut(s) 406
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 5 cut(s) 441, 444, 447, 450, 453
Sau3AI GATC 2 cut(s) 150, 211
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 345, 346
SetI ASST 7 cut(s) 31, 39, 73, 89, 112, 331, 488
SfaNI GCATC 3 cut(s) 291, 386, 464
SmaI CCCGGG 1 cut(s) 346
SmiMI CAYNNNNRTG 1 cut(s) 263
Sse9I AATT 1 cut(s) 414
SsiI CCGC 1 cut(s) 447
SspMI CTAG 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 343, 344
TaaI ACNGT 7 cut(s) 25, 82, 148, 271, 277, 283, 354
TaqI TCGA 3 cut(s) 40, 153, 407
TasI AATT 1 cut(s) 414
TauI GCSGC 1 cut(s) 449
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseFI GTSAC 2 cut(s) 271, 277
TseI GCWGC 4 cut(s) 440, 443, 449, 452
Tsp45I GTSAC 2 cut(s) 271, 277
TspDTI ATGAA 1 cut(s) 339
TspMI CCCGGG 1 cut(s) 344
Tth111I GACNNNGTC 1 cut(s) 283
XapI RAATTY 1 cut(s) 414
XmaI CCCGGG 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.