MD15G1369800.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002479
Physical Location & Seq
Reverse (-)
44948095 .. 44950811
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1369800.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGAGTGCGTGGTCCAAGGAATTATAGAAACACAGCATGTTGAGGCCCTTGAGATTCTTCTTCAGGGTCTTTGTGGTGTTAGCAAGAAAAATTTGAGGGTCCATGAGATATGCCTTAAAAATGGCCCAAACCTTGGGCTTGTAGCATCAGAGGTTCGACTTATATGCGATATTGAACAAAGTGAACAAACTGAACCAACATGGACTATAAGGCATATAGGGGGTGCTATGAGAGGTGCAGGTGCTGAGCAACTTTCAGTGCTAGTAAGATCCGTGGTTGAAAGCAAAACAAGCAAGAATGTGCTTCGCTTTTTTTATGCACTTGGATACAAGTTGGACCATGAGCTGCTAAGGGTGGGATTCTCCTTCCATTTCCAGAGGGGCACGCAGATCAATGTGACGGTGTCATCAGTTAATAAGATGCTTCAACTACATGACATAGATGCGGCTGTCCCTGTAACTCCTGGGATACAGCTGGTTGAAGTAACAGCTCCTGCTACATCTGAAAACTATGCTGAAGTTGTTGCTGCAGTATCATCTTTTAGTGAATATCTTGCACCGCTCCTGCATTTGTCAAAACCGGGCACTTCAACTGGGGTTGTTCCTACTGCTGCTGCGGCTGCTGCATCTCTTTTATCAGATGGTGGGGGTACGACCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000414 GO:0000428 GO:0001101 GO:0002831 GO:0003002 GO:0003006 GO:0003674 GO:0003712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006353 GO:0006355 GO:0006366 GO:0006369 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010219 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0023051 GO:0030880 GO:0031047 GO:0031050 GO:0031056 GO:0031060 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031399 GO:0031554 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032991 GO:0033043 GO:0033044 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043244 GO:0043254 GO:0043331 GO:0043900 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048441 GO:0048442 GO:0048443 GO:0048464 GO:0048465 GO:0048466 GO:0048467 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061458 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090567 GO:0090575 GO:0097305 GO:0097659 GO:0099402 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000113 GO:2000142 GO:2000241 GO:2001023 GO:2001038 GO:2001141 GO:2001253
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

23.48

Weight (kDa)

5.79

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med18 PF09637 65 - 193 7.9e-06 Med18 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 661
AarI CACCTGC 1 cut(s) 230
Acc36I ACCTGC 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 134
AccBSI CCGCTC 1 cut(s) 562
AciI CCGC 3 cut(s) 446, 560, 617
AclWI GGATC 1 cut(s) 264
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 2 cut(s) 47, 537
AfaI GTAC 1 cut(s) 652
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 5 cut(s) 176, 281, 428, 482, 591
AjnI CCWGG 1 cut(s) 463
AluBI AGCT 3 cut(s) 346, 475, 491
AluI AGCT 3 cut(s) 346, 475, 491
AlwI GGATC 1 cut(s) 264
AlwNI CAGNNNCTG 2 cut(s) 245, 494
AoxI GGCC 2 cut(s) 45, 124
ApeKI GCWGC 6 cut(s) 346, 527, 611, 614, 620, 623
ApoI RAATTY 1 cut(s) 91
AspS9I GGNCC 5 cut(s) 13, 46, 100, 125, 337
AsuC2I CCSGG 1 cut(s) 582
AvaII GGWCC 3 cut(s) 13, 100, 337
BaeGI GKGCMC 2 cut(s) 386, 587
BbvI GCAGC 6 cut(s) 333, 514, 598, 601, 607, 610
BccI CCATC 1 cut(s) 635
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 1 cut(s) 465
BciVI GTATCC 2 cut(s) 320, 462
BcnI CCSGG 1 cut(s) 582
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 528
BfuAI ACCTGC 1 cut(s) 230
BfuI GTATCC 2 cut(s) 320, 462
BisI GCNGC 8 cut(s) 347, 447, 528, 612, 615, 618, 621, 624
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 8 cut(s) 348, 448, 529, 613, 616, 619, 622, 625
Bme1390I CCNGG 2 cut(s) 465, 582
Bme18I GGWCC 3 cut(s) 13, 100, 337
BmgT120I GGNCC 5 cut(s) 13, 46, 100, 125, 337
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 465, 582
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 4 cut(s) 155, 411, 433, 635
BmuI ACTGGG 1 cut(s) 603
Bpu10I CCTNAGC 1 cut(s) 350
Bpu1102I GCTNAGC 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 582
BsaJI CCNNGG 4 cut(s) 16, 133, 273, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 598
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 4 cut(s) 16, 133, 273, 464
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 598
BseSI GKGCMC 2 cut(s) 386, 587
BseXI GCAGC 6 cut(s) 333, 514, 598, 601, 607, 610
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 2 cut(s) 47, 126
BsiSI CCGG 1 cut(s) 581
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 2 cut(s) 47, 126
Bsp1286I GDGCHC 2 cut(s) 386, 587
Bsp143I GATC 2 cut(s) 269, 390
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 3 cut(s) 446, 560, 617
BspANI GGCC 2 cut(s) 47, 126
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 101
BspMAI CTGCAG 1 cut(s) 532
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 264
BsrBI CCGCTC 1 cut(s) 562
BsrI ACTGG 1 cut(s) 598
BssECI CCNNGG 4 cut(s) 16, 133, 273, 464
BssMI GATC 2 cut(s) 269, 390
BssT1I CCWWGG 2 cut(s) 16, 133
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 386
BstDEI CTNAG 2 cut(s) 246, 350
BstDSI CCRYGG 1 cut(s) 273
BstKTI GATC 2 cut(s) 272, 393
BstMBI GATC 2 cut(s) 269, 390
BstMWI GCNNNNNNNGC 4 cut(s) 291, 617, 620, 623
BstNI CCWGG 1 cut(s) 465
BstNSI RCATGY 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 463, 580
BstSFI CTRYAG 1 cut(s) 528
BstSLI GKGCMC 2 cut(s) 386, 587
BstV1I GCAGC 6 cut(s) 333, 514, 598, 601, 607, 610
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
BsuI GTATCC 2 cut(s) 320, 462
BsuRI GGCC 2 cut(s) 47, 126
BtgI CCRYGG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 264
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 386
CaiI CAGNNNCTG 2 cut(s) 245, 494
Cfr13I GGNCC 5 cut(s) 13, 46, 100, 125, 337
Csp6I GTAC 1 cut(s) 651
CviAII CATG 5 cut(s) 38, 104, 201, 341, 434
CviJI RGCY 8 cut(s) 47, 126, 139, 346, 449, 475, 491, 620
CviKI_1 RGCY 8 cut(s) 47, 126, 139, 346, 449, 475, 491, 620
CviQI GTAC 1 cut(s) 651
DdeI CTNAG 2 cut(s) 246, 350
DpnI GATC 2 cut(s) 271, 392
DpnII GATC 2 cut(s) 269, 390
Eco130I CCWWGG 2 cut(s) 16, 133
Eco47I GGWCC 3 cut(s) 13, 100, 337
Eco57I CTGAAG 2 cut(s) 47, 537
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 463
EcoT14I CCWWGG 2 cut(s) 16, 133
ErhI CCWWGG 2 cut(s) 16, 133
FaeI CATG 5 cut(s) 41, 107, 204, 344, 437
FaqI GGGAC 1 cut(s) 437
FatI CATG 5 cut(s) 37, 103, 200, 340, 433
Fnu4HI GCNGC 8 cut(s) 347, 447, 528, 612, 615, 618, 621, 624
Fsp4HI GCNGC 8 cut(s) 347, 447, 528, 612, 615, 618, 621, 624
FspBI CTAG 1 cut(s) 263
GluI GCNGC 8 cut(s) 347, 447, 528, 612, 615, 618, 621, 624
HaeIII GGCC 2 cut(s) 47, 126
HapII CCGG 1 cut(s) 581
Hin1II CATG 5 cut(s) 41, 107, 204, 344, 437
HinfI GANTC 2 cut(s) 55, 360
HpaII CCGG 1 cut(s) 581
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 3 cut(s) 151, 505, 640
Hpy188III TCNNGA 1 cut(s) 376
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4V TGCA 6 cut(s) 239, 320, 530, 557, 568, 626
HpyF10VI GCNNNNNNNGC 4 cut(s) 291, 617, 620, 623
HpyF3I CTNAG 2 cut(s) 246, 350
Hsp92II CATG 5 cut(s) 41, 107, 204, 344, 437
Kzo9I GATC 2 cut(s) 269, 390
LmnI GCTCC 2 cut(s) 496, 567
Lsp1109I GCAGC 6 cut(s) 333, 514, 598, 601, 607, 610
LweI GCATC 4 cut(s) 155, 411, 433, 635
MaeI CTAG 1 cut(s) 263
MaeIII GTNAC 3 cut(s) 397, 457, 484
MalI GATC 2 cut(s) 271, 392
MbiI CCGCTC 1 cut(s) 562
MboI GATC 2 cut(s) 269, 390
MboII GAAGA 2 cut(s) 50, 53
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 2 cut(s) 386, 587
MluCI AATT 2 cut(s) 21, 91
MmeI TCCRAC 1 cut(s) 315
MnlI CCTC 5 cut(s) 37, 90, 145, 227, 372
MseI TTAA 2 cut(s) 117, 414
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 1 cut(s) 581
MspR9I CCNGG 2 cut(s) 465, 582
MvaI CCWGG 1 cut(s) 465
MwoI GCNNNNNNNGC 4 cut(s) 291, 617, 620, 623
NciI CCSGG 1 cut(s) 582
NdeII GATC 2 cut(s) 269, 390
NlaIII CATG 5 cut(s) 41, 107, 204, 344, 437
NlaIV GGNNCC 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 397
NspI RCATGY 1 cut(s) 41
PaqCI CACCTGC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 55, 360
PflFI GACNNNGTC 1 cut(s) 403
PflMI CCANNNNNTGG 1 cut(s) 134
PkrI GCNGC 8 cut(s) 348, 448, 529, 613, 616, 619, 622, 625
PsiI TTATAA 1 cut(s) 661
Psp6I CCWGG 1 cut(s) 463
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 5 cut(s) 13, 46, 100, 125, 337
PstI CTGCAG 1 cut(s) 532
PstNI CAGNNNCTG 2 cut(s) 245, 494
PsuI RGATCY 1 cut(s) 269
PsyI GACNNNGTC 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 1 cut(s) 652
RsaNI GTAC 1 cut(s) 651
SaqAI TTAA 2 cut(s) 117, 414
SatI GCNGC 8 cut(s) 347, 447, 528, 612, 615, 618, 621, 624
Sau3AI GATC 2 cut(s) 269, 390
Sau96I GGNCC 5 cut(s) 13, 46, 100, 125, 337
ScrFI CCNGG 2 cut(s) 465, 582
SduI GDGCHC 2 cut(s) 386, 587
SetI ASST 8 cut(s) 135, 156, 238, 244, 348, 477, 493, 659
SfaNI GCATC 4 cut(s) 155, 411, 433, 635
SfcI CTRYAG 1 cut(s) 528
SinI GGWCC 3 cut(s) 13, 100, 337
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 2 cut(s) 21, 91
SsiI CCGC 3 cut(s) 446, 560, 617
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 463, 580
StyI CCWWGG 2 cut(s) 16, 133
TaaI ACNGT 1 cut(s) 403
TaqI TCGA 1 cut(s) 157
TasI AATT 2 cut(s) 21, 91
TauI GCSGC 2 cut(s) 449, 620
TfiI GAWTC 2 cut(s) 55, 360
Tru1I TTAA 2 cut(s) 117, 414
Tru9I TTAA 2 cut(s) 117, 414
TscAI CASTG 1 cut(s) 264
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 6 cut(s) 346, 527, 611, 614, 620, 623
Tsp45I GTSAC 1 cut(s) 397
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 1 cut(s) 264
Tth111I GACNNNGTC 1 cut(s) 403
Van91I CCANNNNNTGG 1 cut(s) 134
VpaK11BI GGWCC 3 cut(s) 13, 100, 337
XapI RAATTY 1 cut(s) 91
XceI RCATGY 1 cut(s) 41
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.