Rh5CG317600

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002479
Physical Location & Seq
Forward (+)
35175949 .. 35183664
7716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG317600.1

Sequence Viewer

Length: 642 bp
ATGGTTTACGTCTTGCAGCATGTTGAAGCCCTTGAGATTCTTCTTCAGGGTCTTTGTGGTGTTCGCAGAGAACCTTTGAAGGTCCATGAGATATGCCTTAAAAGTGGCCCAAACTTAGGAGCTGTACCTTCTGAGGTTCGACTTATTTGCAATCTTGAACAAACTGAACCTACATGGACTGTGAGGTTCGTGGGGGGTGCGATGAGAGGTGCAGGTGCTGAGCAACTCTCAGTTTTAGTAAGAACTGTGATCGAAAGCAAAGCAAGCAAGAATGTGCTTCGCTTATTTTATGCACTTGGCTACAAGTTGGATCATGAGTTACTGAGGGTTGGGTTTGCGTTCCATTTCCAGAGAGGCACGGAGATGACTGTGACTGTGTCATCAGTTAATAAGATGCCACAACTACACAATACAGATGAAGCTGTCCCTGTAACTCCCGGGATACAGTTGGTTGAAGTTACAGCCCCTGCATCATCTGAAACTTATACTGAAGTTGCGTCAGCAGTGTCATCATTTTGCGAATATCTTGCACCCCTCCTGCATTTGTCAAAACCGGGAATTTCAACTGGGGTTGTTCCTACTGCTGCTGCCGCTGCTGCTGCTGCATCTCTCATGTCTGATGGTGGGGGTACTACCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000414 GO:0000428 GO:0001101 GO:0002831 GO:0003002 GO:0003006 GO:0003674 GO:0003712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006353 GO:0006355 GO:0006366 GO:0006369 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010219 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0023051 GO:0030880 GO:0031047 GO:0031050 GO:0031056 GO:0031060 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031399 GO:0031554 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032991 GO:0033043 GO:0033044 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043244 GO:0043254 GO:0043331 GO:0043900 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048441 GO:0048442 GO:0048443 GO:0048464 GO:0048465 GO:0048466 GO:0048467 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061458 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090567 GO:0090575 GO:0097305 GO:0097659 GO:0099402 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000113 GO:2000142 GO:2000241 GO:2001023 GO:2001038 GO:2001141 GO:2001253
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

22.69

Weight (kDa)

6.07

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 203
AciI CCGC 1 cut(s) 591
AclWI GGATC 1 cut(s) 318
AcsI RAATTY 1 cut(s) 558
AcuI CTGAAG 2 cut(s) 29, 510
AfaI GTAC 2 cut(s) 126, 631
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 5 cut(s) 26, 79, 158, 455, 564
AluBI AGCT 2 cut(s) 122, 422
AluI AGCT 2 cut(s) 122, 422
AlwI GGATC 1 cut(s) 318
AlwNI CAGNNNCTG 2 cut(s) 218, 467
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 7 cut(s) 16, 584, 587, 593, 596, 599, 602
ApoI RAATTY 1 cut(s) 558
AspS9I GGNCC 2 cut(s) 82, 107
AsuC2I CCSGG 3 cut(s) 438, 439, 555
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 1 cut(s) 82
BbvI GCAGC 7 cut(s) 28, 571, 574, 580, 583, 586, 589
BccI CCATC 1 cut(s) 614
BcgI CGANNNNNNTGC 4 cut(s) 129, 163, 509, 543
BciVI GTATCC 1 cut(s) 435
BcnI CCSGG 3 cut(s) 438, 439, 555
BfuAI ACCTGC 1 cut(s) 203
BfuI GTATCC 1 cut(s) 435
BisI GCNGC 8 cut(s) 17, 585, 588, 591, 594, 597, 600, 603
BlpI GCTNAGC 1 cut(s) 219
BlsI GCNGC 8 cut(s) 18, 586, 589, 592, 595, 598, 601, 604
Bme1390I CCNGG 3 cut(s) 438, 439, 555
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 82, 107
BmrFI CCNGG 3 cut(s) 438, 439, 555
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 3 cut(s) 384, 479, 614
BmuI ACTGGG 1 cut(s) 576
BplI GAGNNNNNCTC 2 cut(s) 212, 244
Bpu1102I GCTNAGC 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 3 cut(s) 438, 439, 555
BsaJI CCNNGG 1 cut(s) 437
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 571
BseDI CCNNGG 1 cut(s) 437
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 4 cut(s) 123, 210, 243, 314
BseNI ACTGG 1 cut(s) 571
BseXI GCAGC 7 cut(s) 28, 571, 574, 580, 583, 586, 589
BsgI GTGCAG 1 cut(s) 231
BshFI GGCC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 2 cut(s) 438, 554
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 437
Bsp143I GATC 2 cut(s) 249, 310
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 1 cut(s) 591
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 4 cut(s) 124, 211, 242, 315
BspHI TCATGA 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 203
BspPI GGATC 1 cut(s) 318
BsrI ACTGG 1 cut(s) 571
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 2 cut(s) 249, 310
Bst4CI ACNGT 5 cut(s) 181, 247, 370, 376, 447
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 5 cut(s) 115, 132, 219, 229, 323
BstKTI GATC 2 cut(s) 252, 313
BstMBI GATC 2 cut(s) 249, 310
BstMWI GCNNNNNNNGC 6 cut(s) 264, 590, 593, 596, 599, 602
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 3 cut(s) 436, 437, 553
BstV1I GCAGC 7 cut(s) 28, 571, 574, 580, 583, 586, 589
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 108
BtgZI GCGATG 1 cut(s) 215
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 1 cut(s) 510
BveI ACCTGC 1 cut(s) 203
Cac8I GCNNGC 1 cut(s) 265
CaiI CAGNNNCTG 2 cut(s) 218, 467
CciI TCATGA 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 82, 107
Cfr9I CCCGGG 1 cut(s) 437
CseI GACGC 1 cut(s) 486
Csp6I GTAC 2 cut(s) 125, 630
CviAII CATG 5 cut(s) 20, 86, 174, 314, 613
CviJI RGCY 6 cut(s) 29, 108, 122, 300, 422, 464
CviKI_1 RGCY 6 cut(s) 29, 108, 122, 300, 422, 464
CviQI GTAC 2 cut(s) 125, 630
DdeI CTNAG 5 cut(s) 115, 132, 219, 229, 323
DpnI GATC 2 cut(s) 251, 312
DpnII GATC 2 cut(s) 249, 310
Eco47I GGWCC 1 cut(s) 82
Eco57I CTGAAG 2 cut(s) 29, 510
Eco88I CYCGRG 1 cut(s) 437
FaeI CATG 5 cut(s) 23, 89, 177, 317, 616
FaiI YATR 9 cut(s) 21, 87, 94, 175, 291, 315, 486, 614, 640
FaqI GGGAC 1 cut(s) 410
FatI CATG 5 cut(s) 19, 85, 173, 313, 612
Fnu4HI GCNGC 8 cut(s) 17, 585, 588, 591, 594, 597, 600, 603
Fsp4HI GCNGC 8 cut(s) 17, 585, 588, 591, 594, 597, 600, 603
GluI GCNGC 8 cut(s) 17, 585, 588, 591, 594, 597, 600, 603
HaeIII GGCC 1 cut(s) 108
HapII CCGG 2 cut(s) 438, 554
HgaI GACGC 1 cut(s) 486
Hin1II CATG 5 cut(s) 23, 89, 177, 317, 616
HinfI GANTC 1 cut(s) 37
HpaII CCGG 2 cut(s) 438, 554
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 3 cut(s) 133, 478, 619
Hpy188III TCNNGA 3 cut(s) 155, 314, 349
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 2 cut(s) 73, 138
HpyCH4III ACNGT 5 cut(s) 181, 247, 370, 376, 447
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 8 cut(s) 16, 150, 212, 293, 470, 530, 541, 605
HpyF10VI GCNNNNNNNGC 6 cut(s) 264, 590, 593, 596, 599, 602
HpyF3I CTNAG 5 cut(s) 115, 132, 219, 229, 323
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 5 cut(s) 23, 89, 177, 317, 616
Kzo9I GATC 2 cut(s) 249, 310
LmnI GCTCC 1 cut(s) 119
LpnPI CCDG 9 cut(s) 32, 198, 362, 441, 451, 480, 551, 552, 567
Lsp1109I GCAGC 7 cut(s) 28, 571, 574, 580, 583, 586, 589
LweI GCATC 3 cut(s) 384, 479, 614
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 4 cut(s) 318, 370, 430, 457
MalI GATC 2 cut(s) 251, 312
MboI GATC 2 cut(s) 249, 310
MboII GAAGA 2 cut(s) 32, 35
MluCI AATT 1 cut(s) 558
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 6 cut(s) 127, 177, 200, 318, 347, 545
MseI TTAA 2 cut(s) 99, 387
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 2 cut(s) 438, 554
MspR9I CCNGG 3 cut(s) 438, 439, 555
MwoI GCNNNNNNNGC 6 cut(s) 264, 590, 593, 596, 599, 602
NciI CCSGG 3 cut(s) 438, 439, 555
NdeII GATC 2 cut(s) 249, 310
NlaIII CATG 5 cut(s) 23, 89, 177, 317, 616
NmuCI GTSAC 1 cut(s) 370
NspI RCATGY 1 cut(s) 23
PagI TCATGA 1 cut(s) 313
PaqCI CACCTGC 1 cut(s) 203
PfeI GAWTC 1 cut(s) 37
PflFI GACNNNGTC 1 cut(s) 376
PkrI GCNGC 8 cut(s) 18, 586, 589, 592, 595, 598, 601, 604
PspPI GGNCC 2 cut(s) 82, 107
PstNI CAGNNNCTG 2 cut(s) 218, 467
PsyI GACNNNGTC 1 cut(s) 376
RsaI GTAC 2 cut(s) 126, 631
RsaNI GTAC 2 cut(s) 125, 630
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 2 cut(s) 99, 387
SatI GCNGC 8 cut(s) 17, 585, 588, 591, 594, 597, 600, 603
Sau3AI GATC 2 cut(s) 249, 310
Sau96I GGNCC 2 cut(s) 82, 107
ScrFI CCNGG 3 cut(s) 438, 439, 555
SfaNI GCATC 3 cut(s) 384, 479, 614
SinI GGWCC 1 cut(s) 82
SmaI CCCGGG 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 1 cut(s) 558
SsiI CCGC 1 cut(s) 591
StyD4I CCNGG 3 cut(s) 436, 437, 553
TaaI ACNGT 5 cut(s) 181, 247, 370, 376, 447
TaiI ACGT 1 cut(s) 12
TaqI TCGA 2 cut(s) 139, 252
TasI AATT 1 cut(s) 558
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 2 cut(s) 99, 387
Tru9I TTAA 2 cut(s) 99, 387
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 7 cut(s) 16, 584, 587, 593, 596, 599, 602
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 432
TspGWI ACGGA 1 cut(s) 374
TspMI CCCGGG 1 cut(s) 437
TspRI CASTG 1 cut(s) 510
Tth111I GACNNNGTC 1 cut(s) 376
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 1 cut(s) 558
XceI RCATGY 1 cut(s) 23
XmaI CCCGGG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.