mediator of RNA polymerase II transcription subunit
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1063 |
| AatII | GACGTC | 1 cut(s) | 1022 |
| Acc36I | ACCTGC | 1 cut(s) | 927 |
| AccB1I | GGYRCC | 1 cut(s) | 481 |
| AccI | GTMKAC | 1 cut(s) | 47 |
| AccII | CGCG | 1 cut(s) | 1063 |
| AciI | CCGC | 3 cut(s) | 548, 851, 1063 |
| AclWI | GGATC | 4 cut(s) | 93, 171, 654, 881 |
| AcoI | YGGCCR | 3 cut(s) | 1203, 1209, 1221 |
| AcsI | RAATTY | 1 cut(s) | 460 |
| AcuI | CTGAAG | 2 cut(s) | 407, 774 |
| AcyI | GRCGYC | 1 cut(s) | 1019 |
| AfaI | GTAC | 1 cut(s) | 241 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 551, 614, 819, 820, 821 |
| AgsI | TTSAA | 7 cut(s) | 116, 149, 170, 276, 374, 392, 1151 |
| AhlI | ACTAGT | 1 cut(s) | 1241 |
| AjnI | CCWGG | 6 cut(s) | 139, 360, 889, 925, 1159, 1249 |
| AluBI | AGCT | 8 cut(s) | 106, 124, 504, 719, 765, 842, 1080, 1235 |
| AluI | AGCT | 8 cut(s) | 106, 124, 504, 719, 765, 842, 1080, 1235 |
| Alw26I | GTCTC | 4 cut(s) | 19, 209, 697, 1049 |
| AlwI | GGATC | 4 cut(s) | 93, 171, 654, 881 |
| AoxI | GGCC | 6 cut(s) | 53, 358, 1162, 1203, 1209, 1221 |
| ApeKI | GCWGC | 2 cut(s) | 731, 842 |
| ApoI | RAATTY | 1 cut(s) | 460 |
| AspS9I | GGNCC | 2 cut(s) | 358, 1247 |
| AsuC2I | CCSGG | 2 cut(s) | 615, 799 |
| AsuHPI | GGTGA | 3 cut(s) | 43, 1064, 1154 |
| AvaII | GGWCC | 1 cut(s) | 1247 |
| AxyI | CCTNAGG | 1 cut(s) | 57 |
| BaeGI | GKGCMC | 2 cut(s) | 270, 296 |
| BalI | TGGCCA | 1 cut(s) | 1205 |
| BanI | GGYRCC | 1 cut(s) | 481 |
| BbsI | GAAGAC | 1 cut(s) | 156 |
| BbvI | GCAGC | 2 cut(s) | 718, 829 |
| BccI | CCATC | 3 cut(s) | 538, 823, 950 |
| BceAI | ACGGC | 2 cut(s) | 872, 1196 |
| BciT130I | CCWGG | 6 cut(s) | 141, 362, 891, 927, 1161, 1251 |
| BcnI | CCSGG | 2 cut(s) | 615, 799 |
| BcoDI | GTCTC | 4 cut(s) | 19, 209, 697, 1049 |
| BcuI | ACTAGT | 1 cut(s) | 1241 |
| BfaI | CTAG | 3 cut(s) | 468, 933, 1242 |
| BfmI | CTRYAG | 2 cut(s) | 588, 981 |
| BfuAI | ACCTGC | 1 cut(s) | 927 |
| BisI | GCNGC | 2 cut(s) | 732, 843 |
| BlsI | GCNGC | 2 cut(s) | 733, 844 |
| Bme1390I | CCNGG | 8 cut(s) | 141, 362, 615, 799, 891, 927, 1161, 1251 |
| Bme18I | GGWCC | 1 cut(s) | 1247 |
| BmgT120I | GGNCC | 2 cut(s) | 358, 1247 |
| BmiI | GGNNCC | 3 cut(s) | 483, 714, 924 |
| BmrFI | CCNGG | 8 cut(s) | 141, 362, 615, 799, 891, 927, 1161, 1251 |
| BmsI | GCATC | 3 cut(s) | 718, 1087, 1188 |
| BpiI | GAAGAC | 1 cut(s) | 156 |
| BpmI | CTGGAG | 1 cut(s) | 699 |
| BpuEI | CTTGAG | 1 cut(s) | 1014 |
| BpuMI | CCSGG | 2 cut(s) | 615, 799 |
| Bsa29I | ATCGAT | 1 cut(s) | 1008 |
| BsaBI | GATNNNNATC | 2 cut(s) | 84, 645 |
| BsaHI | GRCGYC | 1 cut(s) | 1019 |
| BsaJI | CCNNGG | 3 cut(s) | 1159, 1206, 1224 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 546, 576, 595, 625 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 551, 614, 819, 820, 821 |
| Bse1I | ACTGG | 1 cut(s) | 517 |
| Bse21I | CCTNAGG | 1 cut(s) | 57 |
| Bse8I | GATNNNNATC | 2 cut(s) | 84, 645 |
| BseBI | CCWGG | 6 cut(s) | 141, 362, 891, 927, 1161, 1251 |
| BseCI | ATCGAT | 1 cut(s) | 1008 |
| BseDI | CCNNGG | 3 cut(s) | 1159, 1206, 1224 |
| BseGI | GGATG | 5 cut(s) | 433, 499, 637, 809, 961 |
| BseJI | GATNNNNATC | 2 cut(s) | 84, 645 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 551, 614, 819, 820, 821 |
| BseMII | CTCAG | 6 cut(s) | 71, 112, 587, 957, 1140, 1167 |
| BseNI | ACTGG | 1 cut(s) | 517 |
| BseRI | GAGGAG | 2 cut(s) | 365, 1202 |
| BseSI | GKGCMC | 2 cut(s) | 270, 296 |
| BseXI | GCAGC | 2 cut(s) | 718, 829 |
| Bsh1236I | CGCG | 1 cut(s) | 1063 |
| BshFI | GGCC | 6 cut(s) | 55, 360, 1164, 1205, 1211, 1223 |
| BshNI | GGYRCC | 1 cut(s) | 481 |
| BshVI | ATCGAT | 1 cut(s) | 1008 |
| BsiSI | CCGG | 2 cut(s) | 614, 798 |
| BslFI | GGGAC | 1 cut(s) | 1030 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 551, 614, 819, 820, 821 |
| BsmAI | GTCTC | 4 cut(s) | 19, 209, 697, 1049 |
| BsmFI | GGGAC | 1 cut(s) | 1030 |
| BsmI | GAATGC | 4 cut(s) | 156, 179, 411, 447 |
| BsnI | GGCC | 6 cut(s) | 55, 360, 1164, 1205, 1211, 1223 |
| Bsp1286I | GDGCHC | 2 cut(s) | 270, 296 |
| Bsp1407I | TGTACA | 1 cut(s) | 239 |
| Bsp143I | GATC | 8 cut(s) | 85, 163, 212, 640, 646, 737, 886, 1009 |
| Bsp19I | CCATGG | 1 cut(s) | 1206 |
| BspACI | CCGC | 3 cut(s) | 548, 851, 1063 |
| BspANI | GGCC | 6 cut(s) | 55, 360, 1164, 1205, 1211, 1223 |
| BspCNI | CTCAG | 6 cut(s) | 70, 111, 586, 958, 1139, 1168 |
| BspDI | ATCGAT | 1 cut(s) | 1008 |
| BspFNI | CGCG | 1 cut(s) | 1063 |
| BspLI | GGNNCC | 3 cut(s) | 483, 714, 924 |
| BspMAI | CTGCAG | 1 cut(s) | 985 |
| BspMI | ACCTGC | 1 cut(s) | 927 |
| BspPI | GGATC | 4 cut(s) | 93, 171, 654, 881 |
| BspT107I | GGYRCC | 1 cut(s) | 481 |
| BsrGI | TGTACA | 1 cut(s) | 239 |
| BsrI | ACTGG | 1 cut(s) | 517 |
| BssECI | CCNNGG | 3 cut(s) | 1159, 1206, 1224 |
| BssMI | GATC | 8 cut(s) | 85, 163, 212, 640, 646, 737, 886, 1009 |
| BssNAI | GTATAC | 1 cut(s) | 48 |
| BssNI | GRCGYC | 1 cut(s) | 1019 |
| BssT1I | CCWWGG | 1 cut(s) | 1206 |
| Bst1107I | GTATAC | 1 cut(s) | 48 |
| Bst2UI | CCWGG | 6 cut(s) | 141, 362, 891, 927, 1161, 1251 |
| Bst4CI | ACNGT | 2 cut(s) | 592, 747 |
| Bst6I | CTCTTC | 1 cut(s) | 426 |
| BstACI | GRCGYC | 1 cut(s) | 1019 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 842, 1198 |
| BstAUI | TGTACA | 1 cut(s) | 239 |
| BstC8I | GCNNGC | 5 cut(s) | 126, 981, 1078, 1102, 1203 |
| BstDEI | CTNAG | 8 cut(s) | 57, 98, 120, 345, 573, 966, 1126, 1176 |
| BstDSI | CCRYGG | 1 cut(s) | 1206 |
| BstEII | GGTNACC | 1 cut(s) | 1142 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 549 |
| BstF5I | GGATG | 5 cut(s) | 433, 499, 637, 809, 961 |
| BstFNI | CGCG | 1 cut(s) | 1063 |
| BstKTI | GATC | 8 cut(s) | 88, 166, 215, 643, 649, 740, 889, 1012 |
| BstMAI | GTCTC | 4 cut(s) | 19, 209, 697, 1049 |
| BstMBI | GATC | 8 cut(s) | 85, 163, 212, 640, 646, 737, 886, 1009 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 183, 231, 300, 842, 848, 1198 |
| BstNI | CCWGG | 6 cut(s) | 141, 362, 891, 927, 1161, 1251 |
| BstPI | GGTNACC | 1 cut(s) | 1142 |
| BstSCI | CCNGG | 8 cut(s) | 139, 360, 613, 797, 889, 925, 1159, 1249 |
| BstSFI | CTRYAG | 2 cut(s) | 588, 981 |
| BstSLI | GKGCMC | 2 cut(s) | 270, 296 |
| BstUI | CGCG | 1 cut(s) | 1063 |
| BstV1I | GCAGC | 2 cut(s) | 718, 829 |
| BstV2I | GAAGAC | 1 cut(s) | 156 |
| BstX2I | RGATCY | 2 cut(s) | 163, 646 |
| BstYI | RGATCY | 2 cut(s) | 163, 646 |
| BstZ17I | GTATAC | 1 cut(s) | 48 |
| Bsu15I | ATCGAT | 1 cut(s) | 1008 |
| Bsu36I | CCTNAGG | 1 cut(s) | 57 |
| BsuRI | GGCC | 6 cut(s) | 55, 360, 1164, 1205, 1211, 1223 |
| BsuTUI | ATCGAT | 1 cut(s) | 1008 |
| BtgI | CCRYGG | 1 cut(s) | 1206 |
| BtsCI | GGATG | 5 cut(s) | 433, 499, 637, 809, 961 |
| BtsI | GCAGTG | 1 cut(s) | 296 |
| BtsIMutI | CAGTG | 2 cut(s) | 296, 597 |
| BveI | ACCTGC | 1 cut(s) | 927 |
| Cac8I | GCNNGC | 5 cut(s) | 126, 981, 1078, 1102, 1203 |
| Cfr13I | GGNCC | 2 cut(s) | 358, 1247 |
| ClaI | ATCGAT | 1 cut(s) | 1008 |
| Csp6I | GTAC | 1 cut(s) | 240 |
| CviAII | CATG | 6 cut(s) | 132, 226, 478, 582, 667, 1207 |
| CviQI | GTAC | 1 cut(s) | 240 |
| DdeI | CTNAG | 8 cut(s) | 57, 98, 120, 345, 573, 966, 1126, 1176 |
| DpnI | GATC | 8 cut(s) | 87, 165, 214, 642, 648, 739, 888, 1011 |
| DpnII | GATC | 8 cut(s) | 85, 163, 212, 640, 646, 737, 886, 1009 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1063 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1063 |
| EaeI | YGGCCR | 3 cut(s) | 1203, 1209, 1221 |
| Eam1104I | CTCTTC | 1 cut(s) | 426 |
| EarI | CTCTTC | 1 cut(s) | 426 |
| Eco130I | CCWWGG | 1 cut(s) | 1206 |
| Eco147I | AGGCCT | 1 cut(s) | 55 |
| Eco47I | GGWCC | 1 cut(s) | 1247 |
| Eco57I | CTGAAG | 2 cut(s) | 407, 774 |
| Eco81I | CCTNAGG | 1 cut(s) | 57 |
| Eco91I | GGTNACC | 1 cut(s) | 1142 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 549 |
| EcoO65I | GGTNACC | 1 cut(s) | 1142 |
| EcoRII | CCWGG | 6 cut(s) | 139, 360, 889, 925, 1159, 1249 |
| EcoT14I | CCWWGG | 1 cut(s) | 1206 |
| ErhI | CCWWGG | 1 cut(s) | 1206 |
| FaeI | CATG | 6 cut(s) | 135, 229, 481, 585, 670, 1210 |
| FaqI | GGGAC | 1 cut(s) | 1030 |
| FatI | CATG | 6 cut(s) | 131, 225, 477, 581, 666, 1206 |
| FblI | GTMKAC | 1 cut(s) | 47 |
| Fnu4HI | GCNGC | 2 cut(s) | 732, 843 |
| FokI | GGATG | 5 cut(s) | 440, 506, 644, 796, 968 |
| Fsp4HI | GCNGC | 2 cut(s) | 732, 843 |
| FspBI | CTAG | 3 cut(s) | 468, 933, 1242 |
| GluI | GCNGC | 2 cut(s) | 732, 843 |
| GsuI | CTGGAG | 1 cut(s) | 699 |
| HaeIII | GGCC | 6 cut(s) | 55, 360, 1164, 1205, 1211, 1223 |
| HapII | CCGG | 2 cut(s) | 614, 798 |
| Hin1I | GRCGYC | 1 cut(s) | 1019 |
| Hin1II | CATG | 6 cut(s) | 135, 229, 481, 585, 670, 1210 |
| HindIII | AAGCTT | 1 cut(s) | 122 |
| HinfI | GANTC | 7 cut(s) | 116, 384, 578, 770, 875, 962, 1027 |
| HpaII | CCGG | 2 cut(s) | 614, 798 |
| HphI | GGTGA | 3 cut(s) | 43, 1064, 1154 |
| Hpy166II | GTNNAC | 2 cut(s) | 48, 1247 |
| Hpy188I | TCNGA | 6 cut(s) | 17, 742, 967, 997, 1084, 1129 |
| Hpy8I | GTNNAC | 2 cut(s) | 48, 1247 |
| HpyAV | CCTTC | 4 cut(s) | 349, 545, 798, 1157 |
| HpyCH4III | ACNGT | 2 cut(s) | 592, 747 |
| HpyCH4IV | ACGT | 1 cut(s) | 1019 |
| HpyCH4V | TGCA | 9 cut(s) | 156, 225, 256, 303, 445, 836, 983, 1076, 1115 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 183, 231, 300, 842, 848, 1198 |
| HpyF3I | CTNAG | 8 cut(s) | 57, 98, 120, 345, 573, 966, 1126, 1176 |
| HpySE526I | ACGT | 1 cut(s) | 1019 |
| Hsp92I | GRCGYC | 1 cut(s) | 1019 |
| Hsp92II | CATG | 6 cut(s) | 135, 229, 481, 585, 670, 1210 |
| Kzo9I | GATC | 8 cut(s) | 85, 163, 212, 640, 646, 737, 886, 1009 |
| LmnI | GCTCC | 4 cut(s) | 133, 617, 718, 1189 |
| Lsp1109I | GCAGC | 2 cut(s) | 718, 829 |
| LweI | GCATC | 3 cut(s) | 718, 1087, 1188 |
| MaeI | CTAG | 3 cut(s) | 468, 933, 1242 |
| MaeII | ACGT | 1 cut(s) | 1019 |
| MaeIII | GTNAC | 4 cut(s) | 508, 751, 1142, 1237 |
| MalI | GATC | 8 cut(s) | 87, 165, 214, 642, 648, 739, 888, 1011 |
| MboI | GATC | 8 cut(s) | 85, 163, 212, 640, 646, 737, 886, 1009 |
| MboII | GAAGA | 8 cut(s) | 161, 182, 386, 443, 456, 525, 561, 1106 |
| MflI | RGATCY | 2 cut(s) | 163, 646 |
| MhlI | GDGCHC | 2 cut(s) | 270, 296 |
| MlsI | TGGCCA | 1 cut(s) | 1205 |
| MluCI | AATT | 9 cut(s) | 180, 271, 313, 369, 399, 460, 675, 970, 998 |
| MluNI | TGGCCA | 1 cut(s) | 1205 |
| MlyI | GAGTC | 3 cut(s) | 393, 764, 1021 |
| Mox20I | TGGCCA | 1 cut(s) | 1205 |
| MscI | TGGCCA | 1 cut(s) | 1205 |
| MseI | TTAA | 1 cut(s) | 915 |
| MslI | CAYNNNNRTG | 1 cut(s) | 78 |
| Msp20I | TGGCCA | 1 cut(s) | 1205 |
| MspI | CCGG | 2 cut(s) | 614, 798 |
| MspR9I | CCNGG | 8 cut(s) | 141, 362, 615, 799, 891, 927, 1161, 1251 |
| Mva1269I | GAATGC | 4 cut(s) | 156, 179, 411, 447 |
| MvaI | CCWGG | 6 cut(s) | 141, 362, 891, 927, 1161, 1251 |
| MvnI | CGCG | 1 cut(s) | 1063 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 183, 231, 300, 842, 848, 1198 |
| NciI | CCSGG | 2 cut(s) | 615, 799 |
| NcoI | CCATGG | 1 cut(s) | 1206 |
| NdeII | GATC | 8 cut(s) | 85, 163, 212, 640, 646, 737, 886, 1009 |
| NlaIII | CATG | 6 cut(s) | 135, 229, 481, 585, 670, 1210 |
| NlaIV | GGNNCC | 3 cut(s) | 483, 714, 924 |
| NmeAIII | GCCGAG | 1 cut(s) | 1249 |
| NmuCI | GTSAC | 3 cut(s) | 751, 1142, 1237 |
| PceI | AGGCCT | 1 cut(s) | 55 |
| PctI | GAATGC | 4 cut(s) | 156, 179, 411, 447 |
| PfeI | GAWTC | 4 cut(s) | 116, 578, 875, 962 |
| PfoI | TCCNGGA | 2 cut(s) | 139, 613 |
| PkrI | GCNGC | 2 cut(s) | 733, 844 |
| PleI | GAGTC | 3 cut(s) | 392, 764, 1021 |
| PpsI | GAGTC | 3 cut(s) | 392, 764, 1021 |
| Psp6I | CCWGG | 6 cut(s) | 139, 360, 889, 925, 1159, 1249 |
| PspEI | GGTNACC | 1 cut(s) | 1142 |
| PspGI | CCWGG | 6 cut(s) | 139, 360, 889, 925, 1159, 1249 |
| PspN4I | GGNNCC | 3 cut(s) | 483, 714, 924 |
| PspPI | GGNCC | 2 cut(s) | 358, 1247 |
| PstI | CTGCAG | 1 cut(s) | 985 |
| PsuI | RGATCY | 2 cut(s) | 163, 646 |
| RsaI | GTAC | 1 cut(s) | 241 |
| RsaNI | GTAC | 1 cut(s) | 240 |
| RseI | CAYNNNNRTG | 1 cut(s) | 78 |
| SaqAI | TTAA | 1 cut(s) | 915 |
| SatI | GCNGC | 2 cut(s) | 732, 843 |
| Sau3AI | GATC | 8 cut(s) | 85, 163, 212, 640, 646, 737, 886, 1009 |
| Sau96I | GGNCC | 2 cut(s) | 358, 1247 |
| SchI | GAGTC | 3 cut(s) | 393, 764, 1021 |
| ScrFI | CCNGG | 8 cut(s) | 141, 362, 615, 799, 891, 927, 1161, 1251 |
| SduI | GDGCHC | 2 cut(s) | 270, 296 |
| SfaNI | GCATC | 3 cut(s) | 718, 1087, 1188 |
| SfcI | CTRYAG | 2 cut(s) | 588, 981 |
| SinI | GGWCC | 1 cut(s) | 1247 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 78 |
| SmlI | CTYRAG | 1 cut(s) | 1029 |
| SmoI | CTYRAG | 1 cut(s) | 1029 |
| SpeI | ACTAGT | 1 cut(s) | 1241 |
| Sse9I | AATT | 9 cut(s) | 180, 271, 313, 369, 399, 460, 675, 970, 998 |
| SseBI | AGGCCT | 1 cut(s) | 55 |
| SsiI | CCGC | 3 cut(s) | 548, 851, 1063 |
| SspMI | CTAG | 3 cut(s) | 468, 933, 1242 |
| StuI | AGGCCT | 1 cut(s) | 55 |
| StyD4I | CCNGG | 8 cut(s) | 139, 360, 613, 797, 889, 925, 1159, 1249 |
| StyI | CCWWGG | 1 cut(s) | 1206 |
| TaaI | ACNGT | 2 cut(s) | 592, 747 |
| TaiI | ACGT | 1 cut(s) | 1022 |
| TaqI | TCGA | 4 cut(s) | 736, 908, 1008, 1025 |
| TasI | AATT | 9 cut(s) | 180, 271, 313, 369, 399, 460, 675, 970, 998 |
| TatI | WGTACW | 1 cut(s) | 239 |
| TfiI | GAWTC | 4 cut(s) | 116, 578, 875, 962 |
| Tru1I | TTAA | 1 cut(s) | 915 |
| Tru9I | TTAA | 1 cut(s) | 915 |
| TscAI | CASTG | 2 cut(s) | 296, 597 |
| TseFI | GTSAC | 3 cut(s) | 751, 1142, 1237 |
| TseI | GCWGC | 2 cut(s) | 731, 842 |
| Tsp45I | GTSAC | 3 cut(s) | 751, 1142, 1237 |
| TspDTI | ATGAA | 6 cut(s) | 242, 275, 444, 510, 570, 648 |
| TspRI | CASTG | 2 cut(s) | 296, 597 |
| VpaK11BI | GGWCC | 1 cut(s) | 1247 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 549 |
| XapI | RAATTY | 1 cut(s) | 460 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 319 |
| XmiI | GTMKAC | 1 cut(s) | 47 |
| XspI | CTAG | 3 cut(s) | 468, 933, 1242 |
| ZraI | GACGTC | 1 cut(s) | 1020 |