Rh1BG039200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000070
Physical Location & Seq
Reverse (-)
5177557 .. 5181615
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG039200.1

Sequence Viewer

Length: 2643 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTGGGGAGATCCTTGGCTACTTTGGAAGCTTGGGAGGAGTTGGCAAAGAGCTGCAGAAATGGAGGGGAACACTGTCCGCAATTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAACAAGGATGTGGATCTCTGGCTGAATGATCTCAGACACTTGGCTTATGATATCGACGATCTACTCAGCACCTTTTCTAATGAGATGTTAACCCGCAAGCAAGGTACAAGCAAGTACTTGCGGGTGCTCTCCTTCAATGGCTATAAACTGACCGAGGTGCCAAATTCCGTAGGTAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGTGTTTGCCTGAGTCAACATGCATGCTCTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATGCCTTCATTGGAAGAAATGCCTCCCAAAGTAAGTCGGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGATTGGAGAGATACAGTCATTGCATCTTCGTGGGACCTTGAGTGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGGAAATCTTGCAATTGGAATGGAGCGGCACAAGCGAGAAGGAGTTGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTAAGGAGCTGACCATCAAGGGCTACAATGGGTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGTCTGTGAAATTGGAGAATTGTAAAAACTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAAAACTTCAGATACAAGGACTGGCCAATGTTGAAATTGTGGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGTTAGAATGCATGACGTTCCAGGACATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTGGAGTTTTCCCATGCATGGAAAAGCTTTCCATCCTTGGTTGCCCGAAATTGAAAGGTTGGTTGCCCAAGAGCATGGATTCATTATCAGAGCTGTATATTGCTTTATGTGAAGAGTTAGTGGTTTCAATTGCCAATTATAAACATCTTCGTGGGCTGTTCATCTGCTGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGCTACTGGAGGATATGGAGCTTCATGGTATTTCAAAGTTCAGACTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAACTTTGCGGATTACTGGTTGTGAGGAGTTGACATGTTTATGGGAGAATGAGGATAGATTGCTGCAGCGTCGAATTTCTAATATTATTGGAGGCAACATATCTGACTCCCCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAAGGAACTTGTGCTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGCTAGTTGGCCACCTTGTCTAGAAAATGTAAAGATGGAGTCTTGTAATTCTTTGACATATTTCGTAAGGTATCAAGTACCGCCAAGCATAAGAAGAATAGAGATAGAAAAATGTCAAAATTTGAAACTACTAGTGAAGGATGCTGGGGAGCTAGAGGGTTGTATGGAGTGGTTGGAGATAAAAGAGTGTGCATCTTTGACATCCTTATCAGGCAAAGGAGGCCGACTACCCAGAACCCTCAAATTTCTTAGAATAAGTGACTGTGAACGATTGGAGTCAATAATCAAAACATTCCACGAGGACACTTGTCTTGAAGCTCTTACGATAGAGAGGTGTGCAGATCTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCCGCTCTTCGCATGTTATGGGTAGAGGAATGTGGAAGTCTCGTTTCCTTCCCAAGAGGAGGGTTGCCATCCAACCTGGCATGCTTGTCTATCTCCATTTGTGCTCAATTGGAAGCCCTCCCCAGAGGCATTGACAACCTCAGCTCTCTTCAGATTTTGTTGTTGAGATGCTGGGGAGGTTTGGCATCCATTCTAGAAAAGGGTTTCCCACCCAACCTACTTAATCTGGAAATTGGCCCTCTCTCAGAGTGGGGGCTTCACCACTTGGACAGACTCACCTCTCTTGAAACCTTTTCTATCTACTGTGTAGATCCAGATGTGGTGTCCTTTCCGCCAAAGGAGGTGCTGCTCCCTAAATCTCTCATTAAACTCACCATTGCAGGCTTCCCAAATCTGAAGCGCCTGTCATCATCTTTTCAATCACTCACCTCTCTTGAATCCCTTGACATTCTTGGATGCCCAAAGCTAGCATCTATTATTCCAGAAAAAGAGGATCATCTGCCTTCTTCACTTACACAGCTTCGCATCCAGGACGGCTGTCCGCTGCTCACAAAGAAATACCAACCAGGTAAAGCACGACACTGGCCCAAACAAATTGCCCACATCCCTTACGTTTATGTTGGAGGCTGCGATGACGAAGCCGAAGCCGACCGCCGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

880

Amino Acids

98.71

Weight (kDa)

6.23

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 96 3.6e-19 Rx N-terminal domain
LRR_14 PF23598 99 - 365 9e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 211 - 330 3.4e-34 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1263
AatII GACGTC 1 cut(s) 905
Acc65I GGTACC 1 cut(s) 375
AccB1I GGYRCC 2 cut(s) 340, 375
AccBSI CCGCTC 2 cut(s) 768, 1985
AclWI GGATC 4 cut(s) 64, 204, 2285, 2481
AcoI YGGCCR 3 cut(s) 952, 993, 1613
AcsI RAATTY 5 cut(s) 346, 860, 1488, 1723, 1847
AcuI CTGAAG 4 cut(s) 20, 962, 2114, 2396
AcyI GRCGYC 1 cut(s) 902
AfaI GTAC 4 cut(s) 289, 299, 377, 1683
AfiI CCNNNNNNNGG 4 cut(s) 950, 1141, 2039, 2634
AflII CTTAAG 1 cut(s) 824
AflIII ACRYGT 2 cut(s) 404, 1448
AhlI ACTAGT 1 cut(s) 1735
AjnI CCWGG 5 cut(s) 61, 1071, 2055, 2508, 2545
AjuI GAANNNNNNNTTGG 2 cut(s) 948, 980
AleI CACNNNNGTG 1 cut(s) 1314
Alw21I GWGCWC 4 cut(s) 312, 1311, 1555, 2086
Alw26I GTCTC 2 cut(s) 358, 2024
Alw44I GTGCAC 1 cut(s) 1307
AlwI GGATC 4 cut(s) 64, 204, 2285, 2481
AlwNI CAGNNNCTG 1 cut(s) 43
AoxI GGCC 6 cut(s) 952, 993, 1613, 1825, 2215, 2564
ApaLI GTGCAC 1 cut(s) 1307
ApoI RAATTY 5 cut(s) 346, 860, 1488, 1723, 1847
AseI ATTAAT 1 cut(s) 398
Asp718I GGTACC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 2382
AspS9I GGNCC 3 cut(s) 663, 2216, 2565
AsuC2I CCSGG 1 cut(s) 2636
AsuHPI GGTGA 4 cut(s) 2231, 2248, 2344, 2398
AsuII TTCGAA 1 cut(s) 858
AsuNHI GCTAGC 1 cut(s) 2446
AvaII GGWCC 1 cut(s) 663
BaeGI GKGCMC 1 cut(s) 1311
BalI TGGCCA 3 cut(s) 954, 995, 1615
BanI GGYRCC 2 cut(s) 340, 375
BauI CACGAG 1 cut(s) 1901
BbsI GAAGAC 2 cut(s) 482, 719
Bbv12I GWGCWC 4 cut(s) 312, 1311, 1555, 2086
BbvCI CCTCAGC 1 cut(s) 2120
BccI CCATC 5 cut(s) 845, 1163, 1633, 1983, 2056
BceAI ACGGC 2 cut(s) 732, 2530
BciT130I CCWGG 5 cut(s) 63, 1073, 2057, 2510, 2547
BcnI CCSGG 1 cut(s) 2636
BcoDI GTCTC 2 cut(s) 358, 2024
BcuI ACTAGT 1 cut(s) 1735
BfaI CTAG 6 cut(s) 1608, 1625, 1736, 1757, 2174, 2447
BfmI CTRYAG 2 cut(s) 113, 1478
BfoI RGCGCY 1 cut(s) 2383
BfrI CTTAAG 1 cut(s) 824
BglII AGATCT 2 cut(s) 15, 1945
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 6 cut(s) 63, 1073, 2057, 2510, 2547, 2636
Bme18I GGWCC 1 cut(s) 663
BmgT120I GGNCC 3 cut(s) 663, 2216, 2565
BmiI GGNNCC 3 cut(s) 342, 377, 664
BmrFI CCNGG 6 cut(s) 63, 1073, 2057, 2510, 2547, 2636
BmtI GCTAGC 1 cut(s) 2450
BoxI GACNNNNGTC 2 cut(s) 1911, 2517
BpiI GAAGAC 2 cut(s) 482, 719
BpmI CTGGAG 1 cut(s) 1358
Bpu10I CCTNAGC 1 cut(s) 2120
Bpu14I TTCGAA 1 cut(s) 858
BpuEI CTTGAG 2 cut(s) 688, 1541
BpuMI CCSGG 1 cut(s) 2636
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 902
BsaJI CCNNGG 4 cut(s) 62, 73, 336, 1159
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 4 cut(s) 950, 1141, 2039, 2634
Bse1I ACTGG 6 cut(s) 996, 1049, 1341, 1435, 1579, 2567
Bse3DI GCAATG 2 cut(s) 647, 2355
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 5 cut(s) 63, 1073, 2057, 2510, 2547
BseDI CCNNGG 4 cut(s) 62, 73, 336, 1159
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 4 cut(s) 950, 1141, 2039, 2634
BseMI GCAATG 2 cut(s) 647, 2355
BseMII CTCAG 6 cut(s) 229, 262, 405, 1008, 2134, 2238
BseNI ACTGG 6 cut(s) 996, 1049, 1341, 1435, 1579, 2567
BseRI GAGGAG 5 cut(s) 111, 182, 936, 1454, 2052
BseSI GKGCMC 1 cut(s) 1311
BseYI CCCAGC 2 cut(s) 1748, 2151
BsgI GTGCAG 3 cut(s) 820, 1054, 1962
Bsh1285I CGRYCG 1 cut(s) 2632
BshFI GGCC 6 cut(s) 954, 995, 1615, 1827, 2217, 2566
BshNI GGYRCC 2 cut(s) 340, 375
BsiEI CGRYCG 1 cut(s) 2632
BsiHKAI GWGCWC 4 cut(s) 312, 1311, 1555, 2086
BsiSI CCGG 1 cut(s) 2635
BslFI GGGAC 1 cut(s) 676
BslI CCNNNNNNNGG 4 cut(s) 950, 1141, 2039, 2634
BsmAI GTCTC 2 cut(s) 358, 2024
BsmFI GGGAC 1 cut(s) 676
BsmI GAATGC 2 cut(s) 29, 1064
BsnI GGCC 6 cut(s) 954, 995, 1615, 1827, 2217, 2566
Bsp119I TTCGAA 1 cut(s) 858
Bsp1286I GDGCHC 4 cut(s) 312, 1311, 1555, 2086
Bsp143I GATC 9 cut(s) 15, 69, 196, 211, 241, 382, 1945, 2290, 2473
BspANI GGCC 6 cut(s) 954, 995, 1615, 1827, 2217, 2566
BspCNI CTCAG 6 cut(s) 228, 261, 406, 1009, 2133, 2237
BspLI GGNNCC 3 cut(s) 342, 377, 664
BspMAI CTGCAG 2 cut(s) 117, 1482
BspOI GCTAGC 1 cut(s) 2450
BspPI GGATC 4 cut(s) 64, 204, 2285, 2481
BspQI GCTCTTC 1 cut(s) 1992
BspT104I TTCGAA 1 cut(s) 858
BspT107I GGYRCC 2 cut(s) 340, 375
BspTI CTTAAG 1 cut(s) 824
BsrBI CCGCTC 2 cut(s) 768, 1985
BsrDI GCAATG 2 cut(s) 647, 2355
BsrI ACTGG 6 cut(s) 996, 1049, 1341, 1435, 1579, 2567
BssECI CCNNGG 4 cut(s) 62, 73, 336, 1159
BssMI GATC 9 cut(s) 15, 69, 196, 211, 241, 382, 1945, 2290, 2473
BssNI GRCGYC 1 cut(s) 902
BssSI CACGAG 1 cut(s) 1901
BssT1I CCWWGG 2 cut(s) 73, 1159
Bst2BI CACGAG 1 cut(s) 1901
Bst2UI CCWGG 5 cut(s) 63, 1073, 2057, 2510, 2547
Bst4CI ACNGT 6 cut(s) 135, 375, 645, 932, 1868, 2285
Bst6I CTCTTC 3 cut(s) 1230, 1992, 2133
BstACI GRCGYC 1 cut(s) 902
BstAFI CTTAAG 1 cut(s) 824
BstBI TTCGAA 1 cut(s) 858
BstC8I GCNNGC 6 cut(s) 281, 428, 1040, 2062, 2362, 2448
BstDEI CTNAG 8 cut(s) 215, 248, 414, 1017, 1547, 1853, 2120, 2224
BstH2I RGCGCY 1 cut(s) 2383
BstHHI GCGC 1 cut(s) 2382
BstKTI GATC 9 cut(s) 18, 72, 199, 214, 244, 385, 1948, 2293, 2476
BstMAI GTCTC 2 cut(s) 358, 2024
BstMBI GATC 9 cut(s) 15, 69, 196, 211, 241, 382, 1945, 2290, 2473
BstMCI CGRYCG 1 cut(s) 2632
BstMWI GCNNNNNNNGC 6 cut(s) 146, 592, 716, 774, 829, 1824
BstNI CCWGG 5 cut(s) 63, 1073, 2057, 2510, 2547
BstNSI RCATGY 7 cut(s) 426, 430, 538, 1081, 1452, 1996, 2064
BstPAI GACNNNNGTC 2 cut(s) 1911, 2517
BstSCI CCNGG 6 cut(s) 61, 1071, 2055, 2508, 2545, 2634
BstSFI CTRYAG 2 cut(s) 113, 1478
BstSLI GKGCMC 1 cut(s) 1311
BstV2I GAAGAC 2 cut(s) 482, 719
BstX2I RGATCY 5 cut(s) 15, 69, 196, 1945, 2290
BstXI CCANNNNNNTGG 1 cut(s) 1198
BstYI RGATCY 5 cut(s) 15, 69, 196, 1945, 2290
BsuRI GGCC 6 cut(s) 954, 995, 1615, 1827, 2217, 2566
BtgZI GCGATG 1 cut(s) 2625
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 4 cut(s) 12, 131, 1056, 2560
Cac8I GCNNGC 6 cut(s) 281, 428, 1040, 2062, 2362, 2448
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 1 cut(s) 2382
Cfr13I GGNCC 3 cut(s) 663, 2216, 2565
CseI GACGC 1 cut(s) 1472
CsiI ACCWGGT 1 cut(s) 2545
Csp6I GTAC 4 cut(s) 288, 298, 376, 1682
CviQI GTAC 4 cut(s) 288, 298, 376, 1682
DdeI CTNAG 8 cut(s) 215, 248, 414, 1017, 1547, 1853, 2120, 2224
DpnI GATC 9 cut(s) 17, 71, 198, 213, 243, 384, 1947, 2292, 2475
DpnII GATC 9 cut(s) 15, 69, 196, 211, 241, 382, 1945, 2290, 2473
EaeI YGGCCR 3 cut(s) 952, 993, 1613
Eam1104I CTCTTC 3 cut(s) 1230, 1992, 2133
EarI CTCTTC 3 cut(s) 1230, 1992, 2133
EciI GGCGGA 1 cut(s) 2301
Eco130I CCWWGG 2 cut(s) 73, 1159
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 663
Eco57I CTGAAG 4 cut(s) 20, 962, 2114, 2396
EcoO109I RGGNCCY 1 cut(s) 663
EcoRII CCWGG 5 cut(s) 61, 1071, 2055, 2508, 2545
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 73, 1159
EcoT22I ATGCAT 3 cut(s) 428, 1064, 1142
ErhI CCWWGG 2 cut(s) 73, 1159
FaqI GGGAC 1 cut(s) 676
FauI CCCGC 2 cut(s) 284, 297
FspBI CTAG 6 cut(s) 1608, 1625, 1736, 1757, 2174, 2447
GlaI GCGC 1 cut(s) 2381
GsaI CCCAGC 2 cut(s) 1752, 2155
GsuI CTGGAG 1 cut(s) 1358
HaeII RGCGCY 1 cut(s) 2383
HaeIII GGCC 6 cut(s) 954, 995, 1615, 1827, 2217, 2566
HapII CCGG 1 cut(s) 2635
HgaI GACGC 1 cut(s) 1472
HhaI GCGC 1 cut(s) 2382
Hin1I GRCGYC 1 cut(s) 902
Hin6I GCGC 1 cut(s) 2380
HinP1I GCGC 1 cut(s) 2380
HincII GTYRAC 5 cut(s) 57, 273, 420, 576, 1446
HindII GTYRAC 5 cut(s) 57, 273, 420, 576, 1446
HindIII AAGCTT 4 cut(s) 88, 821, 1148, 1409
HpaI GTTAAC 2 cut(s) 57, 273
HpaII CCGG 1 cut(s) 2635
HphI GGTGA 4 cut(s) 2231, 2248, 2344, 2398
Hpy166II GTNNAC 7 cut(s) 57, 273, 420, 576, 1309, 1446, 1871
Hpy8I GTNNAC 7 cut(s) 57, 273, 420, 576, 1309, 1446, 1871
Hpy99I CGWCG 2 cut(s) 242, 1488
HpyCH4III ACNGT 6 cut(s) 135, 375, 645, 932, 1868, 2285
HpyCH4IV ACGT 6 cut(s) 404, 446, 514, 902, 1067, 2592
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 592, 716, 774, 829, 1824
HpyF3I CTNAG 8 cut(s) 215, 248, 414, 1017, 1547, 1853, 2120, 2224
HpySE526I ACGT 6 cut(s) 404, 446, 514, 902, 1067, 2592
Hsp92I GRCGYC 1 cut(s) 902
HspAI GCGC 1 cut(s) 2380
KpnI GGTACC 1 cut(s) 379
KspAI GTTAAC 2 cut(s) 57, 273
Kzo9I GATC 9 cut(s) 15, 69, 196, 211, 241, 382, 1945, 2290, 2473
LguI GCTCTTC 1 cut(s) 1992
LmnI GCTCC 5 cut(s) 765, 829, 1348, 1753, 2334
MabI ACCWGGT 1 cut(s) 2545
MaeI CTAG 6 cut(s) 1608, 1625, 1736, 1757, 2174, 2447
MaeII ACGT 6 cut(s) 404, 446, 514, 902, 1067, 2592
MaeIII GTNAC 1 cut(s) 1862
MalI GATC 9 cut(s) 17, 71, 198, 213, 243, 384, 1947, 2292, 2475
MbiI CCGCTC 2 cut(s) 768, 1985
MboI GATC 9 cut(s) 15, 69, 196, 211, 241, 382, 1945, 2290, 2473
MfeI CAATTG 4 cut(s) 141, 755, 1251, 2087
MflI RGATCY 5 cut(s) 15, 69, 196, 1945, 2290
MhlI GDGCHC 4 cut(s) 312, 1311, 1555, 2086
MlsI TGGCCA 3 cut(s) 954, 995, 1615
MluNI TGGCCA 3 cut(s) 954, 995, 1615
MlyI GAGTC 7 cut(s) 425, 571, 1368, 1514, 1652, 1889, 2247
MmeI TCCRAC 5 cut(s) 655, 768, 1758, 2076, 2581
Mox20I TGGCCA 3 cut(s) 954, 995, 1615
Mph1103I ATGCAT 3 cut(s) 428, 1064, 1142
MscI TGGCCA 3 cut(s) 954, 995, 1615
MseI TTAA 8 cut(s) 56, 272, 398, 449, 825, 1323, 2202, 2346
MslI CAYNNNNRTG 4 cut(s) 657, 1272, 1314, 1453
Msp20I TGGCCA 3 cut(s) 954, 995, 1615
MspA1I CMGCKG 3 cut(s) 149, 178, 2524
MspCI CTTAAG 1 cut(s) 824
MspI CCGG 1 cut(s) 2635
MspR9I CCNGG 6 cut(s) 63, 1073, 2057, 2510, 2547, 2636
MunI CAATTG 4 cut(s) 141, 755, 1251, 2087
Mva1269I GAATGC 2 cut(s) 29, 1064
MvaI CCWGG 5 cut(s) 63, 1073, 2057, 2510, 2547
MwoI GCNNNNNNNGC 6 cut(s) 146, 592, 716, 774, 829, 1824
NciI CCSGG 1 cut(s) 2636
NdeII GATC 9 cut(s) 15, 69, 196, 211, 241, 382, 1945, 2290, 2473
NheI GCTAGC 1 cut(s) 2446
NlaIV GGNNCC 3 cut(s) 342, 377, 664
NmuCI GTSAC 1 cut(s) 1862
NsiI ATGCAT 3 cut(s) 428, 1064, 1142
NspI RCATGY 7 cut(s) 426, 430, 538, 1081, 1452, 1996, 2064
NspV TTCGAA 1 cut(s) 858
OliI CACNNNNGTG 1 cut(s) 1314
PaeI GCATGC 2 cut(s) 430, 2064
PciI ACATGT 1 cut(s) 1448
PciSI GCTCTTC 1 cut(s) 1992
PctI GAATGC 2 cut(s) 29, 1064
PfeI GAWTC 3 cut(s) 936, 1202, 2417
PfoI TCCNGGA 2 cut(s) 1071, 2508
PleI GAGTC 7 cut(s) 424, 571, 1368, 1514, 1651, 1888, 2247
PpsI GAGTC 7 cut(s) 424, 571, 1368, 1514, 1651, 1888, 2247
PpuMI RGGWCCY 1 cut(s) 663
PscI ACATGT 1 cut(s) 1448
PshAI GACNNNNGTC 2 cut(s) 1911, 2517
PshBI ATTAAT 1 cut(s) 398
PsiI TTATAA 1 cut(s) 1263
Psp5II RGGWCCY 1 cut(s) 663
Psp6I CCWGG 5 cut(s) 61, 1071, 2055, 2508, 2545
PspFI CCCAGC 2 cut(s) 1748, 2151
PspGI CCWGG 5 cut(s) 61, 1071, 2055, 2508, 2545
PspN4I GGNNCC 3 cut(s) 342, 377, 664
PspPI GGNCC 3 cut(s) 663, 2216, 2565
PspPPI RGGWCCY 1 cut(s) 663
PstI CTGCAG 2 cut(s) 117, 1482
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 5 cut(s) 15, 69, 196, 1945, 2290
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 4 cut(s) 289, 299, 377, 1683
RsaNI GTAC 4 cut(s) 288, 298, 376, 1682
RseI CAYNNNNRTG 4 cut(s) 657, 1272, 1314, 1453
SapI GCTCTTC 1 cut(s) 1992
SaqAI TTAA 8 cut(s) 56, 272, 398, 449, 825, 1323, 2202, 2346
Sau3AI GATC 9 cut(s) 15, 69, 196, 211, 241, 382, 1945, 2290, 2473
Sau96I GGNCC 3 cut(s) 663, 2216, 2565
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 7 cut(s) 425, 571, 1368, 1514, 1652, 1889, 2247
ScrFI CCNGG 6 cut(s) 63, 1073, 2057, 2510, 2547, 2636
SduI GDGCHC 4 cut(s) 312, 1311, 1555, 2086
SexAI ACCWGGT 1 cut(s) 2545
SfcI CTRYAG 2 cut(s) 113, 1478
SfuI TTCGAA 1 cut(s) 858
SinI GGWCC 1 cut(s) 663
SmiMI CAYNNNNRTG 4 cut(s) 657, 1272, 1314, 1453
SmlI CTYRAG 3 cut(s) 667, 824, 1556
SmoI CTYRAG 3 cut(s) 667, 824, 1556
SpeI ACTAGT 1 cut(s) 1735
SphI GCATGC 2 cut(s) 430, 2064
SspI AATATT 2 cut(s) 453, 1498
SspMI CTAG 6 cut(s) 1608, 1625, 1736, 1757, 2174, 2447
StyD4I CCNGG 6 cut(s) 61, 1071, 2055, 2508, 2545, 2634
StyI CCWWGG 2 cut(s) 73, 1159
TaaI ACNGT 6 cut(s) 135, 375, 645, 932, 1868, 2285
TaiI ACGT 6 cut(s) 407, 449, 517, 905, 1070, 2595
TaqI TCGA 5 cut(s) 237, 858, 934, 1377, 1486
TaqII GACCGA 1 cut(s) 350
TatI WGTACW 1 cut(s) 297
TauI GCSGC 2 cut(s) 29, 771
TfiI GAWTC 3 cut(s) 936, 1202, 2417
Tru1I TTAA 8 cut(s) 56, 272, 398, 449, 825, 1323, 2202, 2346
Tru9I TTAA 8 cut(s) 56, 272, 398, 449, 825, 1323, 2202, 2346
TscAI CASTG 4 cut(s) 12, 138, 1056, 2567
TseFI GTSAC 1 cut(s) 1862
Tsp45I GTSAC 1 cut(s) 1862
TspDTI ATGAA 8 cut(s) 531, 743, 876, 1131, 1194, 1273, 1343, 1381
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 4 cut(s) 12, 138, 1056, 2567
Vha464I CTTAAG 1 cut(s) 824
VneI GTGCAC 1 cut(s) 1307
VpaK11BI GGWCC 1 cut(s) 663
VspI ATTAAT 1 cut(s) 398
XapI RAATTY 5 cut(s) 346, 860, 1488, 1723, 1847
XbaI TCTAGA 2 cut(s) 1624, 2173
XceI RCATGY 7 cut(s) 426, 430, 538, 1081, 1452, 1996, 2064
XcmI CCANNNNNNNNNTGG 1 cut(s) 1608
XspI CTAG 6 cut(s) 1608, 1625, 1736, 1757, 2174, 2447
ZraI GACGTC 1 cut(s) 903
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 3 cut(s) 428, 1064, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.