MD11G1032000.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Forward (+)
2804739 .. 2808886
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1032000.v1.1.491

Sequence Viewer

Length: 3822 bp
ATGCCACTTGGAGAGGTTTTTCTTGCGGCGTTTCTACAGTTGCTGCTTGACAGGTTGACCCCTCGCGACATTCTCAACTACTTTGGAAACTTCCGGGGTGTTGGAAAGAAGCTGGAGAAGTGGAGGGCCACGTTAACTACAATCGCAGCAGTGCTGAGCGACGCCGATGAAAGACAACGGACTGACGATGCTGTAAAACTATGGCTCAATAATCTCAGAGACCTTGCTTATGATGTCGAAGACATGTTGGACAAATTTGCGACTCAAATGTTGAAGCGCACGATAGAGGGACGTGATCAAGCCAGCACGAGCAACAAGGTGCGAAACTCACTTTTCAAAATTAAATTGAATTGGAATATGAACTCCGAAATGAAGAAGATTACGGAGCGGTTACAAGACATATCTGACCGAAAAGTTAAGTTTAGCTTGAAAGATACTGGGACGTCTGCTAAGGAATCGCGAAGTCTACCATGTTCAGGCGTGTTAGATGAAAAGCTTCTTGTTGGAAGAGATGGTGACAAAGGGAAGATTATTGAATTGTTGAAAAAGTCTACCAATTTTGGTGTGGTTGCTATAGTTGGCATGCCCGGAGTTGGGAAGACGACACTTGCTCAACTTGTATTCAACAACAAAGATGATGCCATGAAGGAGTTTGAGCTCAAGGTCTGGGTATCTGTGTCCGATGACTTCGATGTTGTGCGAGTGACAAAGGCAATTCTTGAATCAATGAAACCCCTACCCGTTCAAGTGGAGGAGTTTAGTAAAATGCAGCATGATTTGAGTGAGCAATTAAGAGGAAAAAAGTTTTTAATCGTTTTAGATGATGTTTGGAACAAAGGTGACCGTGATCTAAACGATCTCTGGTTAAGACTTAAATCCCCTTTTGGCGTCGGAGCAGGAGGAAGTAAGATTATTGTGACAACCCGTGATTTAAATGTTGCAAAGATTATGGGAGCCACTGGATTTCATAATTTGGAATGTATGGCAAATGATGATTGTTTGGAAATATTTGAGCGACATGCATTCGGGGAAGTTAATAGTGGAAAACCAGTAAATTATGAGTTAATTCGGACAAAAATTGTTGAAAAATGTTGTGGCTTACCATTAGCTGCGAGGACTCTCGGTGGTCTTTTACGTTGCAAAGAAACAGATGAGTGGGGAGAAATATTGGACAACAAACTATGGAATCTCGCAGATAACTGTAACCTTCCCCGCGTACTAGAGTTGAGCTATCACTATCTTCCATCACATTTGAAGAGGTGTTTTGCCTATTGCTCAATACTTCCAAATGACTACGAATTTGGGGAGAAGCAGCTCGTCCTTTTGTGGATGGCAGAGGGTTTGATTCAACAAAAACCTGACAACAATAAACAAATGGAGGATTTGGGCCGCGACTACTTTCGAGAGCTATTAGCAAGGTCGCTGTTTCAAGAATCAAGCAAAAACAATTCACGATATGTAATGCATGACCTCGTTTCTGAGTTAACACGATGGGCTGCAGGAGAAATATGTTTTAGGTTGGAAGATAAGCAAGGTGATAACTTGCAAAGCACTTGCTTTCGAGGGGCTCGCCATTCGTCTTTCATTGCTGGTGAATTTGATGGAGTTAAGAGATTTGAGGACTTTCCAAAAGCTGAACGTTTGCGAACATTCCTGCCACTTTCGCTTTCATATTCCACGGGATGGTTAAAATTTTTGTCTCGTCAGGTTACTTTTAAGCTATTACCACAGTTGCAATACTTACGAGTGCTCTCGTTCAATGACTACAAAATAACTGCGCTGCCAGAGTCAATCGGTGATTTGAGGTTGTTACAGTATCTTGACCTTTCCTACACATATATAACTAGTTTGCCTAAATCGATAAGTACTCTTTACAACTTGCAGACATTGATATTGGAACACTGTTACTATTTGAAGTCATTGCTTGCGGACATGAGTAATTTAATTAATTTGCGCCATCTCAACAACTCATATGTATCTTCGTTGGAAGGAATGCCTCCAAATCTAGGTAGCTTGGTGAATCTCCAATCTTTGCCTAATTTTGTGGTCAGTGGTGGAAGTGATCAATCAGGAATAAGAGAGATAGGGCCCCTATCGCATCTCCGAGGGACATTGTGCATCTCAGGATTGGAGAATGTGACTGATGTCGAGGATGCCAGGAGGGCCAACTTGAAATGCAAGGAGAGGCTTGATTCGTTGGTCCTAAAATGGTCTCATTCAAGCGACACGAGAGAAACGGAATCCGTTGTGCTTGACATGTTAGAGCCTCATACAAAGCTCAAGGAGCTCACCATCAAGAGTTATGCCGGAGAGGAATTTTCATCATGGGTTGGAGGTCCTTTGTTCTCTAATATGGTGCTTGTGTGCTTACAGAAATGTAACAATTGTTTATCGTTGCCTCCTCTCGGACAATTGCCTCATCTCAGGGAACTTTATATTAGTGGAATGAATGCAGTGGAAAGTGTTGGTGCTGAGTTTTATGGAGAGCGTATGTTGCCTTTTCCGGTATTAGAGACTCTTGAGTTTGTGGAAATGCAGCATTGGAAGGAATGGCTTCCTTTCCAAGCCGATCACGAAGGTGGTGCTTTCCCTTGCTTGAAAACACTCTTAGTAAAAAAATGTTCTAAACTGGAGGGTAAACTGCCAGAGAACCTCAATTCTTTAGCTAAGCTTAAAATTGTTGAATGTGAAGAATTAGTGGTTTCAATTGCCAACTGCAAACAACTTCGTCAATTAAACATTGACGGTTGTAAAGTGTTGGTGCATACAGCTGCTAAGGTTGAGTTTGAGTTGTTAGAGTCCTTGTGCCTTTCAAACATTTCGGAGATGATGTCTCTGCAAACAGGGGAGTTGTTGAAGAAGGGATTAACCAAGGTTAGAGATTTGAAGATCAGTGGATGTGAGGAGGTGACGTCTTCACTGAAGAATGAGGAGGGTAGATTATTGCAGCAGTTGACTTCTCTTGGCAGTTTGGAAATTGAAGACAACTCTCGTCTAGTTGAAGAATTGGGAAAAGAAGCTGAGGAGTTGCAAATATCGGAGTGCAAGCTTGAATTTCTGGAGTTAAAAAAGTGCGAAAATCTTTCGAAGCTACTAAAAGGATTAAATCAGCTGTCGTCTCTTCAAGAGCTTCGCATACACGAATGTTCAAGTCTAGTTTCTTTCCCAGATGTTGGTCTACCTTCTCTTAAAGACATCCAGATTACATCGTGCGATTCGTTGATATATTTTGCAAAATTCCAGATTCCCCAAAATCTCAGAAGAACAGAGATAAAAGATTGCAAAAGTTTGAAATCACTAGTAGATGAGGAGGCTGTTGGTTCTTCTTGGTCGTCTTTTTCTCATAGTTGTCTTGAGTATTTACGTATCCGAGGTTGTGAATCTCTGATGTCGTTATCATTGAGTGGCCAGCTTCCCAGGACACTTAAACACCTTGATTTAGAGAATTGTGACCGACTGGAGCTAATCGCAGGGGACGGGTTCTTCCGCGACAACACTAATGATTGTCTTGAATATATTAGGATCTGGAATTGTCAAAATCTGAAATCCTTACCGGATGGCTTATGCCACCTCAGCAATCTTCAAACTCTAGGTATTGGGAACTGTGGAAGTCTTGTTTCCATCCCGAGACTGAGTGGGGGGAGAAGACCCTCCAACCTGAGAAAGATCTGGATCATCGGATGCAACAAATTGGAGGCGTTGCCCGAAGACATGCACAATCTCAACTCTCTTGAGGAATTGAGCATCGACTACCGTGAAGGTCAAGAGCTGCAAGTCATTGTGGGAGTTGGAGTGGGGGTTGCACAGACTCACCTCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1274

Amino Acids

143.77

Weight (kDa)

5.91

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 100 2.7e-22 Rx N-terminal domain
NB-ARC PF00931 172 - 342 2.7e-38 NB-ARC domain
WHD_DRP PF23559 427 - 495 2.1e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 574 - 870 1.8e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 692 - 816 3.2e-38 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 446, 2915
AccB7I CCANNNNNTGG 2 cut(s) 1683, 3173
AccBSI CCGCTC 1 cut(s) 388
AccI GTMKAC 3 cut(s) 466, 551, 3178
AccII CGCG 5 cut(s) 66, 460, 1215, 1392, 3489
AciI CCGC 6 cut(s) 26, 388, 1213, 1390, 1928, 3487
AclI AACGTT 1 cut(s) 1639
AclWI GGATC 2 cut(s) 3530, 3680
AcoI YGGCCR 1 cut(s) 3406
AcsI RAATTY 7 cut(s) 254, 1298, 1595, 1691, 2315, 3053, 3236
AcuI CTGAAG 1 cut(s) 2942
AcyI GRCGYC 4 cut(s) 162, 443, 888, 2912
AfaI GTAC 2 cut(s) 1218, 1867
AfiI CCNNNNNNNGG 7 cut(s) 476, 593, 594, 1683, 2006, 2405, 3173
AflIII ACRYGT 2 cut(s) 243, 2256
AhlI ACTAGT 2 cut(s) 1844, 3298
AjiI CACGTC 1 cut(s) 293
AjnI CCWGG 2 cut(s) 2156, 3416
AleI CACNNNNGTG 1 cut(s) 2577
Alw21I GWGCWC 3 cut(s) 660, 1752, 2289
Alw26I GTCTC 7 cut(s) 213, 1704, 2217, 2507, 2838, 3123, 3622
AlwI GGATC 2 cut(s) 3530, 3680
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 3625
AoxI GGCC 5 cut(s) 126, 1387, 2087, 2163, 3406
ApaI GGGCCC 1 cut(s) 2091
ApoI RAATTY 7 cut(s) 254, 1298, 1595, 1691, 2315, 3053, 3236
ArsI GACNNNNNNTTYG 2 cut(s) 1283, 1315
AseI ATTAAT 1 cut(s) 1947
Asp700I GAANNNNTTC 2 cut(s) 495, 2553
AspLEI GCGC 3 cut(s) 279, 1780, 1956
AspS9I GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
AsuC2I CCSGG 2 cut(s) 95, 588
AsuHPI GGTGA 9 cut(s) 527, 851, 1547, 1604, 1808, 2029, 2281, 2920, 3803
AsuII TTCGAA 1 cut(s) 3086
AvaI CYCGRG 1 cut(s) 3625
AvaII GGWCC 2 cut(s) 2200, 2336
BaeGI GKGCMC 1 cut(s) 2091
BalI TGGCCA 1 cut(s) 3408
BanII GRGCYC 4 cut(s) 660, 1570, 2091, 2289
BauI CACGAG 2 cut(s) 307, 2227
BbsI GAAGAC 6 cut(s) 246, 605, 2907, 2988, 3652, 3714
Bbv12I GWGCWC 3 cut(s) 660, 1752, 2289
BbvCI CCTCAGC 2 cut(s) 3021, 3572
BcgI CGANNNNNNTGC 2 cut(s) 2564, 2598
BciT130I CCWGG 2 cut(s) 2158, 3418
BciVI GTATCC 1 cut(s) 3377
BclI TGATCA 2 cut(s) 295, 2062
BcnI CCSGG 2 cut(s) 95, 588
BcoDI GTCTC 7 cut(s) 213, 1704, 2217, 2507, 2838, 3123, 3622
BcuI ACTAGT 2 cut(s) 1844, 3298
BfaI CTAG 7 cut(s) 1220, 1845, 2006, 2996, 3155, 3299, 3590
BfmI CTRYAG 3 cut(s) 35, 573, 1497
BfuI GTATCC 1 cut(s) 3377
BglI GCCNNNNNGGC 1 cut(s) 2162
BglII AGATCT 1 cut(s) 3666
BlpI GCTNAGC 2 cut(s) 155, 2667
BmcAI AGTACT 1 cut(s) 1867
Bme1390I CCNGG 4 cut(s) 95, 588, 2158, 3418
Bme18I GGWCC 2 cut(s) 2200, 2336
BmeT110I CYCGRG 1 cut(s) 3625
BmgBI CACGTC 1 cut(s) 293
BmgT120I GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
BmiI GGNNCC 3 cut(s) 955, 2089, 2090
BmrFI CCNGG 4 cut(s) 95, 588, 2158, 3418
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 7 cut(s) 178, 628, 2107, 2127, 2143, 3671, 3753
BmuI ACTGGG 1 cut(s) 447
BoxI GACNNNNGTC 1 cut(s) 2144
BpiI GAAGAC 6 cut(s) 246, 605, 2907, 2988, 3652, 3714
BpmI CTGGAG 4 cut(s) 134, 2651, 3080, 3479
Bpu10I CCTNAGC 4 cut(s) 450, 2775, 3021, 3572
Bpu1102I GCTNAGC 2 cut(s) 155, 2667
Bpu14I TTCGAA 1 cut(s) 3086
BpuEI CTTGAG 5 cut(s) 644, 2264, 2540, 3374, 3752
BpuMI CCSGG 2 cut(s) 95, 588
Bsa29I ATCGAT 1 cut(s) 1859
BsaAI YACGTR 1 cut(s) 3365
BsaBI GATNNNNATC 1 cut(s) 3671
BsaHI GRCGYC 4 cut(s) 162, 443, 888, 2912
BsaI GGTCTC 2 cut(s) 213, 2217
BsaJI CCNNGG 6 cut(s) 94, 1677, 2104, 2871, 3370, 3416
BsaWI WCCGGW 2 cut(s) 2503, 3553
BsaXI ACNNNNNCTCC 8 cut(s) 2816, 2846, 2897, 2927, 3777, 3783, 3807, 3813
Bsc4I CCNNNNNNNGG 7 cut(s) 476, 593, 594, 1683, 2006, 2405, 3173
Bse1I ACTGG 5 cut(s) 442, 964, 1049, 2634, 3462
Bse3DI GCAATG 2 cut(s) 1584, 1919
Bse8I GATNNNNATC 1 cut(s) 3671
BseBI CCWGG 2 cut(s) 2158, 3418
BseCI ATCGAT 1 cut(s) 1859
BseDI CCNNGG 6 cut(s) 94, 1677, 2104, 2871, 3370, 3416
BseGI GGATG 8 cut(s) 1335, 1688, 2158, 2903, 3195, 3562, 3621, 3686
BseJI GATNNNNATC 1 cut(s) 3671
BseLI CCNNNNNNNGG 7 cut(s) 476, 593, 594, 1683, 2006, 2405, 3173
BseMI GCAATG 2 cut(s) 1584, 1919
BseNI ACTGG 5 cut(s) 442, 964, 1049, 2634, 3462
BseRI GAGGAG 6 cut(s) 767, 2391, 2918, 2945, 3038, 3323
BseSI GKGCMC 1 cut(s) 2091
Bsh1236I CGCG 5 cut(s) 66, 460, 1215, 1392, 3489
BshFI GGCC 5 cut(s) 128, 1389, 2089, 2165, 3408
BshVI ATCGAT 1 cut(s) 1859
BsiHKAI GWGCWC 3 cut(s) 660, 1752, 2289
BsiHKCI CYCGRG 1 cut(s) 3625
BsiSI CCGG 5 cut(s) 94, 588, 2307, 2504, 3554
BslFI GGGAC 4 cut(s) 303, 454, 2122, 3488
BslI CCNNNNNNNGG 7 cut(s) 476, 593, 594, 1683, 2006, 2405, 3173
BsmAI GTCTC 7 cut(s) 213, 1704, 2217, 2507, 2838, 3123, 3622
BsmBI CGTCTC 1 cut(s) 3123
BsmFI GGGAC 4 cut(s) 303, 454, 2122, 3488
BsmI GAATGC 3 cut(s) 1022, 1998, 2455
BsnI GGCC 5 cut(s) 128, 1389, 2089, 2165, 3408
Bso31I GGTCTC 2 cut(s) 213, 2217
BsoBI CYCGRG 1 cut(s) 3625
Bsp119I TTCGAA 1 cut(s) 3086
Bsp120I GGGCCC 1 cut(s) 2087
Bsp1286I GDGCHC 5 cut(s) 660, 1570, 1752, 2091, 2289
Bsp143I GATC 9 cut(s) 295, 847, 856, 2062, 2569, 2889, 3522, 3666, 3672
Bsp1720I GCTNAGC 2 cut(s) 155, 2667
Bsp68I TCGCGA 2 cut(s) 66, 460
BspACI CCGC 6 cut(s) 26, 388, 1213, 1390, 1928, 3487
BspANI GGCC 5 cut(s) 128, 1389, 2089, 2165, 3408
BspDI ATCGAT 1 cut(s) 1859
BspFNI CGCG 5 cut(s) 66, 460, 1215, 1392, 3489
BspLI GGNNCC 3 cut(s) 955, 2089, 2090
BspMAI CTGCAG 1 cut(s) 1501
BspPI GGATC 2 cut(s) 3530, 3680
BspT104I TTCGAA 1 cut(s) 3086
BspTNI GGTCTC 2 cut(s) 213, 2217
BsrBI CCGCTC 1 cut(s) 388
BsrDI GCAATG 2 cut(s) 1584, 1919
BsrI ACTGG 5 cut(s) 442, 964, 1049, 2634, 3462
BssECI CCNNGG 6 cut(s) 94, 1677, 2104, 2871, 3370, 3416
BssMI GATC 9 cut(s) 295, 847, 856, 2062, 2569, 2889, 3522, 3666, 3672
BssNI GRCGYC 4 cut(s) 162, 443, 888, 2912
BssSI CACGAG 2 cut(s) 307, 2227
BssT1I CCWWGG 1 cut(s) 2871
Bst2BI CACGAG 2 cut(s) 307, 2227
Bst2UI CCWGG 2 cut(s) 2158, 3418
Bst4CI ACNGT 9 cut(s) 39, 845, 1202, 1731, 1815, 1904, 2747, 3605, 3755
Bst6I CTCTTC 3 cut(s) 502, 1250, 3126
BstACI GRCGYC 4 cut(s) 162, 443, 888, 2912
BstBAI YACGTR 1 cut(s) 3365
BstBI TTCGAA 1 cut(s) 3086
BstC8I GCNNGC 6 cut(s) 304, 584, 1570, 1926, 3047, 3410
BstDSI CCRYGG 1 cut(s) 1677
BstEII GGTNACC 1 cut(s) 839
BstF5I GGATG 8 cut(s) 1335, 1688, 2158, 2903, 3195, 3562, 3621, 3686
BstFNI CGCG 5 cut(s) 66, 460, 1215, 1392, 3489
BstHHI GCGC 3 cut(s) 279, 1780, 1956
BstKTI GATC 9 cut(s) 298, 850, 859, 2065, 2572, 2892, 3525, 3669, 3675
BstMAI GTCTC 7 cut(s) 213, 1704, 2217, 2507, 2838, 3123, 3622
BstMBI GATC 9 cut(s) 295, 847, 856, 2062, 2569, 2889, 3522, 3666, 3672
BstMWI GCNNNNNNNGC 6 cut(s) 1663, 2095, 2162, 2284, 2494, 3573
BstNI CCWGG 2 cut(s) 2158, 3418
BstNSI RCATGY 5 cut(s) 247, 586, 1022, 2260, 3715
BstPAI GACNNNNGTC 1 cut(s) 2144
BstPI GGTNACC 1 cut(s) 839
BstSCI CCNGG 4 cut(s) 93, 586, 2156, 3416
BstSFI CTRYAG 3 cut(s) 35, 573, 1497
BstSLI GKGCMC 1 cut(s) 2091
BstSNI TACGTA 1 cut(s) 3365
BstUI CGCG 5 cut(s) 66, 460, 1215, 1392, 3489
BstV2I GAAGAC 6 cut(s) 246, 605, 2907, 2988, 3652, 3714
BstX2I RGATCY 2 cut(s) 3522, 3666
BstYI RGATCY 2 cut(s) 3522, 3666
Bsu15I ATCGAT 1 cut(s) 1859
BsuI GTATCC 1 cut(s) 3377
BsuRI GGCC 5 cut(s) 128, 1389, 2089, 2165, 3408
BsuTUI ATCGAT 1 cut(s) 1859
BtgI CCRYGG 1 cut(s) 1677
BtrI CACGTC 1 cut(s) 293
BtsCI GGATG 8 cut(s) 1335, 1688, 2158, 2903, 3195, 3562, 3621, 3686
BtsI GCAGTG 2 cut(s) 156, 2460
BtsIMutI CAGTG 7 cut(s) 156, 957, 1900, 2056, 2460, 2899, 2918
BtuMI TCGCGA 2 cut(s) 66, 460
Cac8I GCNNGC 6 cut(s) 304, 584, 1570, 1926, 3047, 3410
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 3 cut(s) 279, 1780, 1956
Cfr13I GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
ClaI ATCGAT 1 cut(s) 1859
CseI GACGC 2 cut(s) 170, 877
Csp6I GTAC 2 cut(s) 1217, 1866
CviQI GTAC 2 cut(s) 1217, 1866
DpnI GATC 9 cut(s) 297, 849, 858, 2064, 2571, 2891, 3524, 3668, 3674
DpnII GATC 9 cut(s) 295, 847, 856, 2062, 2569, 2889, 3522, 3666, 3672
DraI TTTAAA 1 cut(s) 933
EaeI YGGCCR 1 cut(s) 3406
Eam1104I CTCTTC 3 cut(s) 502, 1250, 3126
EarI CTCTTC 3 cut(s) 502, 1250, 3126
Ecl136II GAGCTC 2 cut(s) 658, 2287
Eco105I TACGTA 1 cut(s) 3365
Eco130I CCWWGG 1 cut(s) 2871
Eco24I GRGCYC 4 cut(s) 660, 1570, 2091, 2289
Eco31I GGTCTC 2 cut(s) 213, 2217
Eco47I GGWCC 2 cut(s) 2200, 2336
Eco53kI GAGCTC 2 cut(s) 658, 2287
Eco57I CTGAAG 1 cut(s) 2942
Eco88I CYCGRG 1 cut(s) 3625
Eco91I GGTNACC 1 cut(s) 839
EcoICRI GAGCTC 2 cut(s) 658, 2287
EcoO109I RGGNCCY 3 cut(s) 2087, 2088, 2336
EcoO65I GGTNACC 1 cut(s) 839
EcoRII CCWGG 2 cut(s) 2156, 3416
EcoT14I CCWWGG 1 cut(s) 2871
EcoT22I ATGCAT 2 cut(s) 1024, 1467
EcoT38I GRGCYC 4 cut(s) 660, 1570, 2091, 2289
ErhI CCWWGG 1 cut(s) 2871
Esp3I CGTCTC 1 cut(s) 3123
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 4 cut(s) 303, 454, 2122, 3488
FauI CCCGC 1 cut(s) 1220
FauNDI CATATG 1 cut(s) 1972
FbaI TGATCA 2 cut(s) 295, 2062
FblI GTMKAC 3 cut(s) 466, 551, 3178
FokI GGATG 8 cut(s) 1342, 1695, 2165, 2910, 3182, 3569, 3608, 3693
FriOI GRGCYC 4 cut(s) 660, 1570, 2091, 2289
FspBI CTAG 7 cut(s) 1220, 1845, 2006, 2996, 3155, 3299, 3590
GlaI GCGC 3 cut(s) 278, 1779, 1955
GsuI CTGGAG 4 cut(s) 134, 2651, 3080, 3479
HaeIII GGCC 5 cut(s) 128, 1389, 2089, 2165, 3408
HapII CCGG 5 cut(s) 94, 588, 2307, 2504, 3554
HgaI GACGC 2 cut(s) 170, 877
HhaI GCGC 3 cut(s) 279, 1780, 1956
Hin1I GRCGYC 4 cut(s) 162, 443, 888, 2912
Hin6I GCGC 3 cut(s) 277, 1778, 1954
HinP1I GCGC 3 cut(s) 277, 1778, 1954
HincII GTYRAC 4 cut(s) 57, 135, 1485, 2955
HindII GTYRAC 4 cut(s) 57, 135, 1485, 2955
HindIII AAGCTT 3 cut(s) 494, 2669, 3047
HpaI GTTAAC 2 cut(s) 135, 1485
HpaII CCGG 5 cut(s) 94, 588, 2307, 2504, 3554
HphI GGTGA 9 cut(s) 527, 851, 1547, 1604, 1808, 2029, 2281, 2920, 3803
Hpy166II GTNNAC 8 cut(s) 57, 135, 467, 552, 1485, 2639, 2955, 3179
Hpy8I GTNNAC 8 cut(s) 57, 135, 467, 552, 1485, 2639, 2955, 3179
Hpy99I CGWCG 2 cut(s) 164, 893
HpyAV CCTTC 8 cut(s) 640, 1217, 1982, 2539, 2570, 2854, 3192, 3752
HpyCH4III ACNGT 9 cut(s) 39, 845, 1202, 1731, 1815, 1904, 2747, 3605, 3755
HpyCH4IV ACGT 7 cut(s) 131, 292, 443, 1135, 1639, 2912, 3364
HpyF10VI GCNNNNNNNGC 6 cut(s) 1663, 2095, 2162, 2284, 2494, 3573
HpySE526I ACGT 7 cut(s) 131, 292, 443, 1135, 1639, 2912, 3364
Hsp92I GRCGYC 4 cut(s) 162, 443, 888, 2912
HspAI GCGC 3 cut(s) 277, 1778, 1954
Ksp22I TGATCA 2 cut(s) 295, 2062
KspAI GTTAAC 2 cut(s) 135, 1485
Kzo9I GATC 9 cut(s) 295, 847, 856, 2062, 2569, 2889, 3522, 3666, 3672
LmnI GCTCC 5 cut(s) 385, 893, 953, 2284, 3460
LweI GCATC 7 cut(s) 178, 628, 2107, 2127, 2143, 3671, 3753
MaeI CTAG 7 cut(s) 1220, 1845, 2006, 2996, 3155, 3299, 3590
MaeII ACGT 7 cut(s) 131, 292, 443, 1135, 1639, 2912, 3364
MalI GATC 9 cut(s) 297, 849, 858, 2064, 2571, 2891, 3524, 3668, 3674
MbiI CCGCTC 1 cut(s) 388
MboI GATC 9 cut(s) 295, 847, 856, 2062, 2569, 2889, 3522, 3666, 3672
MfeI CAATTG 3 cut(s) 2383, 2411, 2706
MflI RGATCY 2 cut(s) 3522, 3666
MhlI GDGCHC 5 cut(s) 660, 1570, 1752, 2091, 2289
MlsI TGGCCA 1 cut(s) 3408
MluNI TGGCCA 1 cut(s) 3408
MlyI GAGTC 6 cut(s) 256, 1111, 1796, 2509, 2807, 3802
MmeI TCCRAC 9 cut(s) 82, 228, 484, 871, 1500, 1965, 2311, 3678, 3769
Mox20I TGGCCA 1 cut(s) 3408
Mph1103I ATGCAT 2 cut(s) 1024, 1467
MroXI GAANNNNTTC 2 cut(s) 495, 2553
MscI TGGCCA 1 cut(s) 3408
MslI CAYNNNNRTG 1 cut(s) 2577
Msp20I TGGCCA 1 cut(s) 3408
MspA1I CMGCKG 2 cut(s) 2771, 3112
MspI CCGG 5 cut(s) 94, 588, 2307, 2504, 3554
MspR9I CCNGG 4 cut(s) 95, 588, 2158, 3418
MunI CAATTG 3 cut(s) 2383, 2411, 2706
Mva1269I GAATGC 3 cut(s) 1022, 1998, 2455
MvaI CCWGG 2 cut(s) 2158, 3418
MvnI CGCG 5 cut(s) 66, 460, 1215, 1392, 3489
MwoI GCNNNNNNNGC 6 cut(s) 1663, 2095, 2162, 2284, 2494, 3573
NciI CCSGG 2 cut(s) 95, 588
NdeI CATATG 1 cut(s) 1972
NdeII GATC 9 cut(s) 295, 847, 856, 2062, 2569, 2889, 3522, 3666, 3672
NlaIV GGNNCC 3 cut(s) 955, 2089, 2090
NmuCI GTSAC 7 cut(s) 515, 703, 839, 916, 2137, 2908, 3449
NruI TCGCGA 2 cut(s) 66, 460
NsiI ATGCAT 2 cut(s) 1024, 1467
NspI RCATGY 5 cut(s) 247, 586, 1022, 2260, 3715
NspV TTCGAA 1 cut(s) 3086
OliI CACNNNNGTG 1 cut(s) 2577
PacI TTAATTAA 1 cut(s) 1947
PaeI GCATGC 1 cut(s) 586
PciI ACATGT 2 cut(s) 243, 2256
PcsI WCGNNNNNNNCGW 1 cut(s) 3215
PctI GAATGC 3 cut(s) 1022, 1998, 2455
PdmI GAANNNNTTC 2 cut(s) 495, 2553
PflMI CCANNNNNTGG 2 cut(s) 1683, 3173
PleI GAGTC 6 cut(s) 256, 1111, 1795, 2509, 2806, 3802
PpsI GAGTC 6 cut(s) 256, 1111, 1795, 2509, 2806, 3802
Ppu21I YACGTR 1 cut(s) 3365
PpuMI RGGWCCY 1 cut(s) 2336
PscI ACATGT 2 cut(s) 243, 2256
PshAI GACNNNNGTC 1 cut(s) 2144
PshBI ATTAAT 1 cut(s) 1947
Psp124BI GAGCTC 2 cut(s) 660, 2289
Psp1406I AACGTT 1 cut(s) 1639
Psp5II RGGWCCY 1 cut(s) 2336
Psp6I CCWGG 2 cut(s) 2156, 3416
PspEI GGTNACC 1 cut(s) 839
PspGI CCWGG 2 cut(s) 2156, 3416
PspN4I GGNNCC 3 cut(s) 955, 2089, 2090
PspOMI GGGCCC 1 cut(s) 2087
PspPI GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
PspPPI RGGWCCY 1 cut(s) 2336
PstI CTGCAG 1 cut(s) 1501
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 2 cut(s) 3522, 3666
PvuII CAGCTG 2 cut(s) 2771, 3112
RruI TCGCGA 2 cut(s) 66, 460
RsaI GTAC 2 cut(s) 1218, 1867
RsaNI GTAC 2 cut(s) 1217, 1866
RseI CAYNNNNRTG 1 cut(s) 2577
SacI GAGCTC 2 cut(s) 660, 2289
Sau3AI GATC 9 cut(s) 295, 847, 856, 2062, 2569, 2889, 3522, 3666, 3672
Sau96I GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
ScaI AGTACT 1 cut(s) 1867
SchI GAGTC 6 cut(s) 256, 1111, 1796, 2509, 2807, 3802
ScrFI CCNGG 4 cut(s) 95, 588, 2158, 3418
SduI GDGCHC 5 cut(s) 660, 1570, 1752, 2091, 2289
SfaNI GCATC 7 cut(s) 178, 628, 2107, 2127, 2143, 3671, 3753
SfcI CTRYAG 3 cut(s) 35, 573, 1497
SfuI TTCGAA 1 cut(s) 3086
SinI GGWCC 2 cut(s) 2200, 2336
SmiI ATTTAAAT 1 cut(s) 933
SmiMI CAYNNNNRTG 1 cut(s) 2577
SmlI CTYRAG 5 cut(s) 659, 2279, 2519, 3353, 3731
SmoI CTYRAG 5 cut(s) 659, 2279, 2519, 3353, 3731
SnaBI TACGTA 1 cut(s) 3365
SpeI ACTAGT 2 cut(s) 1844, 3298
SphI GCATGC 1 cut(s) 586
SsiI CCGC 6 cut(s) 26, 388, 1213, 1390, 1928, 3487
SspI AATATT 2 cut(s) 1008, 1167
SspMI CTAG 7 cut(s) 1220, 1845, 2006, 2996, 3155, 3299, 3590
SstI GAGCTC 2 cut(s) 660, 2289
StyD4I CCNGG 4 cut(s) 93, 586, 2156, 3416
StyI CCWWGG 1 cut(s) 2871
SwaI ATTTAAAT 1 cut(s) 933
TaaI ACNGT 9 cut(s) 39, 845, 1202, 1731, 1815, 1904, 2747, 3605, 3755
TaiI ACGT 7 cut(s) 134, 295, 446, 1138, 1642, 2915, 3367
TaqI TCGA 8 cut(s) 237, 690, 1402, 1561, 1859, 2148, 3086, 3747
TaqII GACCGA 2 cut(s) 423, 3468
TatI WGTACW 1 cut(s) 1865
TauI GCSGC 2 cut(s) 29, 1392
TscAI CASTG 7 cut(s) 156, 964, 1907, 2056, 2460, 2899, 2925
TseFI GTSAC 7 cut(s) 515, 703, 839, 916, 2137, 2908, 3449
Tsp45I GTSAC 7 cut(s) 515, 703, 839, 916, 2137, 2908, 3449
TspGWI ACGGA 4 cut(s) 193, 398, 2233, 2252
TspRI CASTG 7 cut(s) 156, 964, 1907, 2056, 2460, 2899, 2925
Van91I CCANNNNNTGG 2 cut(s) 1683, 3173
VpaK11BI GGWCC 2 cut(s) 2200, 2336
VspI ATTAAT 1 cut(s) 1947
XapI RAATTY 7 cut(s) 254, 1298, 1595, 1691, 2315, 3053, 3236
XceI RCATGY 5 cut(s) 247, 586, 1022, 2260, 3715
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmiI GTMKAC 3 cut(s) 466, 551, 3178
XmnI GAANNNNTTC 2 cut(s) 495, 2553
XspI CTAG 7 cut(s) 1220, 1845, 2006, 2996, 3155, 3299, 3590
ZraI GACGTC 2 cut(s) 444, 2913
ZrmI AGTACT 1 cut(s) 1867
Zsp2I ATGCAT 2 cut(s) 1024, 1467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.