pycom13g25140

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000070
Physical Location & Seq
Forward (+)
22065701 .. 22069927
4227 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25140.5

Sequence Viewer

Length: 3789 bp
ATGAAGTTTCATACCTCACTTGAAGGAAATCATTGCCGGAATCGCCGGAAAAATGATAAAACCGCCGGAAAAGTCGTGGACTTGAGACCTCGAACTGCAAAGATGGAAAAGAAAAGGGAAAATCTAACATCACAGAGATATAGGGCTCGTCAAGAGCTTTCCATGGACACCAAGATCGATCCAAAACGGAGCTCGGATGAAAACGATACAAGCGGGTCAAGTTTGCTCTTCCCCCCATTTTTCCGAAAAACTCTCCCTTCTTTTTGGAGGTTTCCCTCCCATTTAATTTATAGCAATTTGGTAATATTACCAAAATATCTTCAAGAGTTGACCGAAAAGGTCGAGAAAATTCGGGATGACAAAAGTACCCCTCACTTCAAAATAATAATTTTAGTGACAGGATATCACATATTTTGTATGTATATTTCCATAAAACGTAACAGGGGCTCTGAATCCACCCCATTGCCATCCCTAGCTATTCCCAAATTGTCACCTTTAGAATGCTTTACTACGAGTCCATTAATTGATTATTACACTTTTACTATAAATTTGTATGTGATTTTGTGCCTATTTCTGAGCCCCAATAGATCAAGCTTCCAAATTTCCAAGAAACTAGTCTTTGACTGTTACTCTTCCCGAACGGAGTCAGATTTAACTTCCAAATCATTTGTTTCTTCACTTAATTTGTGCAGTTCTGCACCTCAATATTGCTCAAGGACGGTTCGTTCACTATTTCTTCAACTGCTTGGAGTTCTCTTCGCTCTTAACATGGCACTCGGAGAGGTTTTTCTTGCGGCGTTTCTACAGTTGCTGCTCGACAGGTTGACTCGTCGCGAGGTTCTCAACTATTTTGGAAACTTCCGGGGCGTTGGAAAGAAGCTGGACAAATGGAGGGCCACGTTATCTACAATCGCAGCGGTGCTGAGCGACGCCGAGGAAAGACAACTGACTGACCATCTTGATGTCGAAGACATGTTGGACAAATTTGCAACTCAAATGTTGAAGCGTATGATAGAGGGACGTGTCAGTGCGAGCAACAAGGTACGGAACTCGCTTTTCAAAATTAAATTCAATTGGGATATGAACTCCAAAATGAAGAAGATTACGGAGAGGTTGCAAGACATATCTGAATGGAAAGACAAGTTTGGCTTGAAAGATACTGGGACGTCTGCTAAGGAATCGCAAAGTCTACCAAGTTCAGGCGTATTAGATGAAAAGCTTGTTGTTGGAAGAGATGGTGACAAAGGGGAGATTATTGAATTCTTGTCCAGAAAATACGAGCGTGCAGGTGCTGTCAATTTTGGTGTAGTTGCTATAGTTGGCCTGCCAGGAGTTGGGAAGACGACACTTGCTCAACTTGTATTCAACAACAAAGATGATGCCATGAAGGAGTTTGAGCTCAAGGTCTGGGTATCTGTGTCCGATGACTTCGATGTGGTGCGAGTGACAAAGGCAATTCTTCAATCAATCACATCCCAACCCGTTCAAGTGGAGGAGTTTAGTAAAATGCAGCATGATTTGAGTGAGCAATTAAGAGGAAAAAAGTTTTTAATTGTTTTAGATGATATTTGGGACAAAGATGACCATGATTTATACGATCTCTGGACAAGACTTCAATCCCCTTTTGGCGTCGGAGCAGGAGGAAGTAAGATAATTGTGACAACCCGTGACAAGAATGTTGCAAAGATTATGGGAGCCGCTGGAGTTCATAATTTGGAGTGTATGGCAGATGATGATTGTATGGAAATATTTGAGCGACACGCGTTTGGGGAAAATAATAACGGAAAACCAGTAAATTATGAGGTAATTCGGACGAAAATTGTTGGAAAATGTTGTGGATTACCATTAGCTGCAAGAACTCTCGGTGGTCTTTTACGTCGCCAAGAAAAAGAAGAGTGGGGAGAAATATTGGACAACAAATTATGGAATCTAGCAGATAAGAGTGGCCTTCTTCCTGTACTAAAGTTGAGCTATCACTATCTTCCATCACATTTGAAGAGGTGTTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGAAGGAGCTCGTACTTTTGTGGATGGCAGAGGGTTTGATTCAACAAAAACATGACGACAATAAACAAATGGAGGATTTGGGCCACGACTACTTTCGAGAGCTATTAGCAAGGTCGCTGTTTCAAGAATCAAGCAAAAACAATTCACGATATGTAATGCATGACCTCATTAATGATTTAGCACGATGGGCAGCAGGTGAAATATGTTTTAGATTGGAAGATAAGCAAGGTGATAACTTGCAAAGCACTTGCTTTCGAAGGGCTCGCCATTCGTCTTTCATTGCTGGTCAATTTGATGGAGTTATGAGATTTGAGGACTTTCCAAAAGCTGAACGTTTACGAACATTCCTGCCACTTTCACTTTCAGATTCCAGGGGATGGTACAAATATTTGTCTCGTAAGGTTACTTTTGAGTTATTACCACAGTTGCAATACTTACGAGTGCTCTCTTTCAATGGCTACAAAATAACTGAGCTGCCAAAGTCAATCGGTGATTTGAGGTTGTTACAGTATCTTGACCTATCCTACACATTGATAACCAGTTTGCCTAAATCAACAAGCACTCTTTACTACTTGCAAACATTGATATTGGAAGGTTGTTATAAATTGAAGTCATTGCCTACGGACTTGAGCAATCTAATTAAGTTGCGCCATCTCAACAACTCAGGTGCATATTCGTTGGAAGGAATGCCTTCGCAACTAGGTCGATTGCCGAATCTACAATCACTGCCTCTTTTTGTGGTGAGCGAAGGTAGTGATCAGTCAGGGATAAGAGAGATAGGGCCCCTATTACATCTCCGAGGGACATTGTGCCTCTCAAAATTGGAGAATGTGATTGATGTTGAGGATGCCAGAAGGGCCAACTTGAAATGCAAGGAGAGGCTTGATTCGTTGGTCCTAGAATGGTCTCGTTCAAGGGACACAAGAGAAACAGAATCCACTGTGCTTGGCATGTTACAGCCTCATAGAAAGATCAAGGAGCTCACCATCAAGAGTTATGCCGAAAAGAAATTTTCATCATGGGTTGGAGGTTCTTTCTTCTCTAATATGGTGCTTGTGCGCTTAGAGGAATGTAACAATTGTTTATCGTTGCCACCTCTCGGACAATTGCCTCAACTCAAAGAGCTCTATATTAGAGGAATGAATGCAGTGGAAAGTGTTGGTGCTGAGTTTTATGGCGAGTGTGTCTTGGCTTTTCCGCTGTTAGAGATTCTCGAGTTTGTGGATATGCAACATTGGAAGGTGTGGCTTCCCTTCCAACTGGATCACGGAAGTGGTGTTTTCCCTTGCCTGGAAAGGCTTTCAATAAAAAAATGTTCCAAGTTGGAAGGTAAGCTGCCAGAGAAGCTCGATTTGTTAGCCAAACTTGAAATTGTTAAATGTGAGGAATTGGTGGTTTCGATTGCCAACTACAAACAACTTCGTCAAATAAACATTGACGGTTGTAAAGTATTGGTACATACAGATGCTAAGGTTGAGTTTGAGTTATTAGAGTCCTTGTGGCTTTCAAACATTTCGGAGGTGATGTCTTTACAAACAGGGGAATTATTCAGGAAGGGATTAAGCAAGGTTAGAGATTTGAAGATCAGTGGATGTGAGAAGCTGACGTCTTCATGGAAGAATGAGGGTAGATTATTGCGGCGGTTGACTTGTCTTGGCCGTTTGGAAATTGAAGACAACTCTTGTCTAGTTGAAGAATTGGGAGAAGAAGCAGAGGAGTTGCTGCAATTGGAAATATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1263

Amino Acids

144.26

Weight (kDa)

8.47

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2649
AarI CACCTGC 2 cut(s) 1277, 2231
AatII GACGTC 2 cut(s) 1169, 3656
Acc36I ACCTGC 2 cut(s) 1277, 2231
AccB7I CCANNNNNTGG 2 cut(s) 1334, 2424
AccI GTMKAC 1 cut(s) 1189
AccII CGCG 2 cut(s) 834, 1763
AciI CCGC 8 cut(s) 63, 213, 794, 917, 1698, 3245, 3685, 3688
AclI AACGTT 1 cut(s) 2380
AclWI GGATC 2 cut(s) 173, 3318
AcoI YGGCCR 1 cut(s) 3703
AcsI RAATTY 8 cut(s) 348, 547, 600, 983, 1067, 1259, 2039, 3056
AcyI GRCGYC 4 cut(s) 930, 1166, 1629, 3653
AfaI GTAC 6 cut(s) 367, 1044, 1959, 2061, 2429, 3504
AfiI CCNNNNNNNGG 5 cut(s) 1199, 1334, 2424, 3146, 3337
AflIII ACRYGT 3 cut(s) 972, 1021, 1761
AhlI ACTAGT 1 cut(s) 613
AjiI CACGTC 1 cut(s) 1022
AjnI CCWGG 3 cut(s) 1327, 2417, 3336
AleI CACNNNNGTG 1 cut(s) 3318
AloI GAACNNNNNNTCC 2 cut(s) 709, 741
Alw21I GWGCWC 6 cut(s) 194, 1401, 2058, 2493, 3030, 3174
Alw26I GTCTC 3 cut(s) 79, 2445, 2958
AlwI GGATC 2 cut(s) 173, 3318
AlwNI CAGNNNCTG 2 cut(s) 811, 1292
Ama87I CYCGRG 1 cut(s) 3260
AoxI GGCC 7 cut(s) 894, 1321, 1945, 2128, 2828, 2904, 3703
ApaI GGGCCC 1 cut(s) 2832
ApeKI GCWGC 8 cut(s) 811, 914, 1510, 1850, 2237, 2521, 3382, 3769
ApoI RAATTY 8 cut(s) 348, 547, 600, 983, 1067, 1259, 2039, 3056
ArsI GACNNNNNNTTYG 2 cut(s) 2024, 2056
AseI ATTAAT 2 cut(s) 521, 2217
Asp700I GAANNNNTTC 1 cut(s) 2737
AspLEI GCGC 2 cut(s) 2697, 3108
AspS9I GGNCC 6 cut(s) 894, 2128, 2828, 2829, 2904, 2941
AsuC2I CCSGG 1 cut(s) 863
AsuHPI GGTGA 8 cut(s) 483, 1250, 2255, 2288, 2549, 2800, 3022, 3580
AsuII TTCGAA 1 cut(s) 2302
AvaI CYCGRG 1 cut(s) 3260
AvaII GGWCC 1 cut(s) 2941
BaeGI GKGCMC 1 cut(s) 2832
BaeI ACNNNNGTAYC 2 cut(s) 349, 382
BbsI GAAGAC 4 cut(s) 975, 1346, 3648, 3726
Bbv12I GWGCWC 6 cut(s) 194, 1401, 2058, 2493, 3030, 3174
BbvI GCAGC 8 cut(s) 798, 926, 1522, 1837, 2249, 2508, 3369, 3756
BceAI ACGGC 1 cut(s) 3690
BcgI CGANNNNNNTGC 2 cut(s) 2732, 2766
BciT130I CCWGG 3 cut(s) 1329, 2419, 3338
BclI TGATCA 1 cut(s) 2803
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 3 cut(s) 79, 2445, 2958
BcuI ACTAGT 1 cut(s) 613
BfaI CTAG 6 cut(s) 473, 614, 1931, 2747, 2945, 3734
BfmI CTRYAG 2 cut(s) 803, 1314
BfuAI ACCTGC 2 cut(s) 1277, 2231
BglI GCCNNNNNGGC 1 cut(s) 2903
BlpI GCTNAGC 1 cut(s) 923
Bme1390I CCNGG 4 cut(s) 863, 1329, 2419, 3338
Bme18I GGWCC 1 cut(s) 2941
BmeT110I CYCGRG 1 cut(s) 3260
BmgBI CACGTC 1 cut(s) 1022
BmgT120I GGNCC 6 cut(s) 894, 2128, 2828, 2829, 2904, 2941
BmiI GGNNCC 3 cut(s) 1696, 2830, 2831
BmrFI CCNGG 4 cut(s) 863, 1329, 2419, 3338
BmrI ACTGGG 1 cut(s) 1170
BmsI GCATC 3 cut(s) 1369, 2884, 3502
BmuI ACTGGG 1 cut(s) 1170
BpiI GAAGAC 4 cut(s) 975, 1346, 3648, 3726
BpmI CTGGAG 1 cut(s) 1722
Bpu10I CCTNAGC 2 cut(s) 1173, 3516
Bpu1102I GCTNAGC 1 cut(s) 923
Bpu14I TTCGAA 1 cut(s) 2302
BpuEI CTTGAG 4 cut(s) 103, 697, 1385, 2695
BpuMI CCSGG 1 cut(s) 863
Bsa29I ATCGAT 1 cut(s) 177
BsaHI GRCGYC 4 cut(s) 930, 1166, 1629, 3653
BsaI GGTCTC 2 cut(s) 79, 2958
BsaJI CCNNGG 5 cut(s) 162, 862, 933, 2418, 2845
BsaXI ACNNNNNCTCC 2 cut(s) 3557, 3587
Bsc4I CCNNNNNNNGG 5 cut(s) 1199, 1334, 2424, 3146, 3337
Bse1I ACTGG 4 cut(s) 1165, 1790, 2586, 3312
Bse3DI GCAATG 4 cut(s) 31, 461, 2325, 2660
BseBI CCWGG 3 cut(s) 1329, 2419, 3338
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 5 cut(s) 162, 862, 933, 2418, 2845
BseGI GGATG 8 cut(s) 202, 361, 467, 1472, 2076, 2429, 2899, 3644
BseLI CCNNNNNNNGG 5 cut(s) 1199, 1334, 2424, 3146, 3337
BseMI GCAATG 4 cut(s) 31, 461, 2325, 2660
BseMII CTCAG 5 cut(s) 566, 914, 2508, 2724, 3204
BseNI ACTGG 4 cut(s) 1165, 1790, 2586, 3312
BseRI GAGGAG 2 cut(s) 1508, 3776
BseSI GKGCMC 1 cut(s) 2832
BseXI GCAGC 8 cut(s) 798, 926, 1522, 1837, 2249, 2508, 3369, 3756
BsgI GTGCAG 3 cut(s) 681, 709, 1305
Bsh1236I CGCG 2 cut(s) 834, 1763
BshFI GGCC 7 cut(s) 896, 1323, 1947, 2130, 2830, 2906, 3705
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 6 cut(s) 194, 1401, 2058, 2493, 3030, 3174
BsiHKCI CYCGRG 1 cut(s) 3260
BsiSI CCGG 4 cut(s) 37, 46, 66, 862
BslFI GGGAC 5 cut(s) 1032, 1177, 1586, 2863, 2978
BslI CCNNNNNNNGG 5 cut(s) 1199, 1334, 2424, 3146, 3337
BsmAI GTCTC 3 cut(s) 79, 2445, 2958
BsmFI GGGAC 5 cut(s) 1032, 1177, 1586, 2863, 2978
BsmI GAATGC 3 cut(s) 506, 2739, 3196
BsnI GGCC 7 cut(s) 896, 1323, 1947, 2130, 2830, 2906, 3705
Bso31I GGTCTC 2 cut(s) 79, 2958
BsoBI CYCGRG 1 cut(s) 3260
Bsp119I TTCGAA 1 cut(s) 2302
Bsp120I GGGCCC 1 cut(s) 2828
Bsp143I GATC 8 cut(s) 174, 178, 587, 1597, 2803, 3018, 3310, 3630
Bsp1720I GCTNAGC 1 cut(s) 923
Bsp19I CCATGG 1 cut(s) 162
Bsp68I TCGCGA 1 cut(s) 834
BspACI CCGC 8 cut(s) 63, 213, 794, 917, 1698, 3245, 3685, 3688
BspANI GGCC 7 cut(s) 896, 1323, 1947, 2130, 2830, 2906, 3705
BspCNI CTCAG 5 cut(s) 567, 915, 2509, 2723, 3205
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 2 cut(s) 834, 1763
BspLI GGNNCC 3 cut(s) 1696, 2830, 2831
BspMI ACCTGC 2 cut(s) 1277, 2231
BspPI GGATC 2 cut(s) 173, 3318
BspQI GCTCTTC 1 cut(s) 233
BspT104I TTCGAA 1 cut(s) 2302
BspTNI GGTCTC 2 cut(s) 79, 2958
BsrDI GCAATG 4 cut(s) 31, 461, 2325, 2660
BsrI ACTGG 4 cut(s) 1165, 1790, 2586, 3312
BssECI CCNNGG 5 cut(s) 162, 862, 933, 2418, 2845
BssMI GATC 8 cut(s) 174, 178, 587, 1597, 2803, 3018, 3310, 3630
BssNI GRCGYC 4 cut(s) 930, 1166, 1629, 3653
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 3 cut(s) 1329, 2419, 3338
Bst4CI ACNGT 7 cut(s) 626, 721, 807, 2472, 2556, 2989, 3488
Bst6I CTCTTC 6 cut(s) 233, 637, 761, 1225, 1887, 1991
BstACI GRCGYC 4 cut(s) 930, 1166, 1629, 3653
BstBI TTCGAA 1 cut(s) 2302
BstC8I GCNNGC 4 cut(s) 1033, 1284, 1325, 2311
BstDEI CTNAG 8 cut(s) 575, 923, 1173, 2517, 2710, 3109, 3213, 3516
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 8 cut(s) 202, 361, 467, 1472, 2076, 2429, 2899, 3644
BstFNI CGCG 2 cut(s) 834, 1763
BstHHI GCGC 2 cut(s) 2697, 3108
BstKTI GATC 8 cut(s) 177, 181, 590, 1600, 2806, 3021, 3313, 3633
BstMAI GTCTC 3 cut(s) 79, 2445, 2958
BstMBI GATC 8 cut(s) 174, 178, 587, 1597, 2803, 3018, 3310, 3630
BstMWI GCNNNNNNNGC 4 cut(s) 42, 2234, 2903, 3391
BstNI CCWGG 3 cut(s) 1329, 2419, 3338
BstNSI RCATGY 2 cut(s) 976, 3001
BstSCI CCNGG 4 cut(s) 861, 1327, 2417, 3336
BstSFI CTRYAG 2 cut(s) 803, 1314
BstSLI GKGCMC 1 cut(s) 2832
BstUI CGCG 2 cut(s) 834, 1763
BstV1I GCAGC 8 cut(s) 798, 926, 1522, 1837, 2249, 2508, 3369, 3756
BstV2I GAAGAC 4 cut(s) 975, 1346, 3648, 3726
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 7 cut(s) 896, 1323, 1947, 2130, 2830, 2906, 3705
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 162
BtrI CACGTC 1 cut(s) 1022
BtsCI GGATG 8 cut(s) 202, 361, 467, 1472, 2076, 2429, 2899, 3644
BtsI GCAGTG 2 cut(s) 2771, 3201
BtsIMutI CAGTG 5 cut(s) 1033, 2771, 2985, 3201, 3640
BtuMI TCGCGA 1 cut(s) 834
BveI ACCTGC 2 cut(s) 1277, 2231
Cac8I GCNNGC 4 cut(s) 1033, 1284, 1325, 2311
CaiI CAGNNNCTG 2 cut(s) 811, 1292
CfoI GCGC 2 cut(s) 2697, 3108
Cfr13I GGNCC 6 cut(s) 894, 2128, 2828, 2829, 2904, 2941
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 2 cut(s) 938, 1618
Csp6I GTAC 6 cut(s) 366, 1043, 1958, 2060, 2428, 3503
CspCI CAANNNNNGTGG 2 cut(s) 2974, 3009
CviQI GTAC 6 cut(s) 366, 1043, 1958, 2060, 2428, 3503
DdeI CTNAG 8 cut(s) 575, 923, 1173, 2517, 2710, 3109, 3213, 3516
DpnI GATC 8 cut(s) 176, 180, 589, 1599, 2805, 3020, 3312, 3632
DpnII GATC 8 cut(s) 174, 178, 587, 1597, 2803, 3018, 3310, 3630
EaeI YGGCCR 1 cut(s) 3703
Eam1104I CTCTTC 6 cut(s) 233, 637, 761, 1225, 1887, 1991
EarI CTCTTC 6 cut(s) 233, 637, 761, 1225, 1887, 1991
Ecl136II GAGCTC 5 cut(s) 192, 1399, 2056, 3028, 3172
Eco130I CCWWGG 1 cut(s) 162
Eco31I GGTCTC 2 cut(s) 79, 2958
Eco32I GATATC 1 cut(s) 404
Eco47I GGWCC 1 cut(s) 2941
Eco53kI GAGCTC 5 cut(s) 192, 1399, 2056, 3028, 3172
Eco88I CYCGRG 1 cut(s) 3260
EcoICRI GAGCTC 5 cut(s) 192, 1399, 2056, 3028, 3172
EcoO109I RGGNCCY 2 cut(s) 2828, 2829
EcoRI GAATTC 1 cut(s) 1259
EcoRII CCWGG 3 cut(s) 1327, 2417, 3336
EcoRV GATATC 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 162
EcoT22I ATGCAT 1 cut(s) 2208
ErhI CCWWGG 1 cut(s) 162
FalI AAGNNNNNCTT 2 cut(s) 1133, 1165
FaqI GGGAC 5 cut(s) 1032, 1177, 1586, 2863, 2978
FauI CCCGC 1 cut(s) 206
FbaI TGATCA 1 cut(s) 2803
FblI GTMKAC 1 cut(s) 1189
FokI GGATG 8 cut(s) 209, 368, 454, 1459, 2083, 2436, 2906, 3651
FspBI CTAG 6 cut(s) 473, 614, 1931, 2747, 2945, 3734
GlaI GCGC 2 cut(s) 2696, 3107
GsuI CTGGAG 1 cut(s) 1722
HaeIII GGCC 7 cut(s) 896, 1323, 1947, 2130, 2830, 2906, 3705
HapII CCGG 4 cut(s) 37, 46, 66, 862
HgaI GACGC 2 cut(s) 938, 1618
HhaI GCGC 2 cut(s) 2697, 3108
Hin1I GRCGYC 4 cut(s) 930, 1166, 1629, 3653
Hin6I GCGC 2 cut(s) 2695, 3106
HinP1I GCGC 2 cut(s) 2695, 3106
HincII GTYRAC 3 cut(s) 330, 825, 3693
HindII GTYRAC 3 cut(s) 330, 825, 3693
HindIII AAGCTT 2 cut(s) 592, 1217
HpaII CCGG 4 cut(s) 37, 46, 66, 862
HphI GGTGA 8 cut(s) 483, 1250, 2255, 2288, 2549, 2800, 3022, 3580
Hpy166II GTNNAC 7 cut(s) 79, 330, 728, 825, 1190, 2384, 3693
Hpy8I GTNNAC 7 cut(s) 79, 330, 728, 825, 1190, 2384, 3693
Hpy99I CGWCG 4 cut(s) 834, 932, 1634, 1881
HpyCH4III ACNGT 7 cut(s) 626, 721, 807, 2472, 2556, 2989, 3488
HpyCH4IV ACGT 7 cut(s) 436, 899, 1021, 1166, 1876, 2380, 3653
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 2234, 2903, 3391
HpyF3I CTNAG 8 cut(s) 575, 923, 1173, 2517, 2710, 3109, 3213, 3516
HpySE526I ACGT 7 cut(s) 436, 899, 1021, 1166, 1876, 2380, 3653
Hsp92I GRCGYC 4 cut(s) 930, 1166, 1629, 3653
HspAI GCGC 2 cut(s) 2695, 3106
Ksp22I TGATCA 1 cut(s) 2803
Kzo9I GATC 8 cut(s) 174, 178, 587, 1597, 2803, 3018, 3310, 3630
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 5 cut(s) 189, 1634, 1694, 2053, 3025
Lsp1109I GCAGC 8 cut(s) 798, 926, 1522, 1837, 2249, 2508, 3369, 3756
LweI GCATC 3 cut(s) 1369, 2884, 3502
MaeI CTAG 6 cut(s) 473, 614, 1931, 2747, 2945, 3734
MaeII ACGT 7 cut(s) 436, 899, 1021, 1166, 1876, 2380, 3653
MalI GATC 8 cut(s) 176, 180, 589, 1599, 2805, 3020, 3312, 3632
MboI GATC 8 cut(s) 174, 178, 587, 1597, 2803, 3018, 3310, 3630
MfeI CAATTG 4 cut(s) 1072, 3124, 3152, 3773
MluI ACGCGT 1 cut(s) 1761
MlyI GAGTC 4 cut(s) 523, 653, 820, 3548
MmeI TCCRAC 9 cut(s) 850, 957, 1207, 1612, 1804, 2706, 3052, 3328, 3351
Mph1103I ATGCAT 1 cut(s) 2208
MroXI GAANNNNTTC 1 cut(s) 2737
MslI CAYNNNNRTG 3 cut(s) 960, 3318, 3510
MspA1I CMGCKG 3 cut(s) 917, 1700, 3247
MspI CCGG 4 cut(s) 37, 46, 66, 862
MspR9I CCNGG 4 cut(s) 863, 1329, 2419, 3338
MunI CAATTG 4 cut(s) 1072, 3124, 3152, 3773
Mva1269I GAATGC 3 cut(s) 506, 2739, 3196
MvaI CCWGG 3 cut(s) 1329, 2419, 3338
MvnI CGCG 2 cut(s) 834, 1763
MwoI GCNNNNNNNGC 4 cut(s) 42, 2234, 2903, 3391
NciI CCSGG 1 cut(s) 863
NcoI CCATGG 1 cut(s) 162
NdeII GATC 8 cut(s) 174, 178, 587, 1597, 2803, 3018, 3310, 3630
NlaIV GGNNCC 3 cut(s) 1696, 2830, 2831
NmeAIII GCCGAG 1 cut(s) 958
NmuCI GTSAC 6 cut(s) 394, 489, 1238, 1444, 1657, 1667
NruI TCGCGA 1 cut(s) 834
NsiI ATGCAT 1 cut(s) 2208
NspI RCATGY 2 cut(s) 976, 3001
NspV TTCGAA 1 cut(s) 2302
OliI CACNNNNGTG 1 cut(s) 3318
PaeR7I CTCGAG 1 cut(s) 3260
PaqCI CACCTGC 2 cut(s) 1277, 2231
PciI ACATGT 1 cut(s) 972
PciSI GCTCTTC 1 cut(s) 233
PctI GAATGC 3 cut(s) 506, 2739, 3196
PdmI GAANNNNTTC 1 cut(s) 2737
PflMI CCANNNNNTGG 2 cut(s) 1334, 2424
PleI GAGTC 4 cut(s) 522, 652, 820, 3547
PpsI GAGTC 4 cut(s) 522, 652, 820, 3547
PscI ACATGT 1 cut(s) 972
PshBI ATTAAT 2 cut(s) 521, 2217
PsiI TTATAA 1 cut(s) 2649
Psp124BI GAGCTC 5 cut(s) 194, 1401, 2058, 3030, 3174
Psp1406I AACGTT 1 cut(s) 2380
Psp6I CCWGG 3 cut(s) 1327, 2417, 3336
PspGI CCWGG 3 cut(s) 1327, 2417, 3336
PspN4I GGNNCC 3 cut(s) 1696, 2830, 2831
PspOMI GGGCCC 1 cut(s) 2828
PspPI GGNCC 6 cut(s) 894, 2128, 2828, 2829, 2904, 2941
PstNI CAGNNNCTG 2 cut(s) 811, 1292
RruI TCGCGA 1 cut(s) 834
RsaI GTAC 6 cut(s) 367, 1044, 1959, 2061, 2429, 3504
RsaNI GTAC 6 cut(s) 366, 1043, 1958, 2060, 2428, 3503
RseI CAYNNNNRTG 3 cut(s) 960, 3318, 3510
SacI GAGCTC 5 cut(s) 194, 1401, 2058, 3030, 3174
SapI GCTCTTC 1 cut(s) 233
Sau3AI GATC 8 cut(s) 174, 178, 587, 1597, 2803, 3018, 3310, 3630
Sau96I GGNCC 6 cut(s) 894, 2128, 2828, 2829, 2904, 2941
SchI GAGTC 4 cut(s) 523, 653, 820, 3548
ScrFI CCNGG 4 cut(s) 863, 1329, 2419, 3338
SfaNI GCATC 3 cut(s) 1369, 2884, 3502
SfcI CTRYAG 2 cut(s) 803, 1314
Sfr274I CTCGAG 1 cut(s) 3260
SfuI TTCGAA 1 cut(s) 2302
SinI GGWCC 1 cut(s) 2941
SlaI CTCGAG 1 cut(s) 3260
SmiMI CAYNNNNRTG 3 cut(s) 960, 3318, 3510
SmlI CTYRAG 5 cut(s) 82, 712, 1400, 2674, 3260
SmoI CTYRAG 5 cut(s) 82, 712, 1400, 2674, 3260
SpeI ACTAGT 1 cut(s) 613
SsiI CCGC 8 cut(s) 63, 213, 794, 917, 1698, 3245, 3685, 3688
SspI AATATT 6 cut(s) 306, 707, 1749, 1908, 2435, 3783
SspMI CTAG 6 cut(s) 473, 614, 1931, 2747, 2945, 3734
SstI GAGCTC 5 cut(s) 194, 1401, 2058, 3030, 3174
StyD4I CCNGG 4 cut(s) 861, 1327, 2417, 3336
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 7 cut(s) 626, 721, 807, 2472, 2556, 2989, 3488
TaiI ACGT 7 cut(s) 439, 902, 1024, 1169, 1879, 2383, 3656
TaqII GACCGA 1 cut(s) 347
TatI WGTACW 1 cut(s) 1957
TauI GCSGC 3 cut(s) 797, 1700, 3688
TscAI CASTG 5 cut(s) 1033, 2778, 2992, 3201, 3640
TseFI GTSAC 6 cut(s) 394, 489, 1238, 1444, 1657, 1667
TseI GCWGC 8 cut(s) 811, 914, 1510, 1850, 2237, 2521, 3382, 3769
Tsp45I GTSAC 6 cut(s) 394, 489, 1238, 1444, 1657, 1667
TspGWI ACGGA 7 cut(s) 202, 656, 1060, 1121, 1797, 2684, 3330
TspRI CASTG 5 cut(s) 1033, 2778, 2992, 3201, 3640
Van91I CCANNNNNTGG 2 cut(s) 1334, 2424
VpaK11BI GGWCC 1 cut(s) 2941
VspI ATTAAT 2 cut(s) 521, 2217
XapI RAATTY 8 cut(s) 348, 547, 600, 983, 1067, 1259, 2039, 3056
XceI RCATGY 2 cut(s) 976, 3001
XhoI CTCGAG 1 cut(s) 3260
XmiI GTMKAC 1 cut(s) 1189
XmnI GAANNNNTTC 1 cut(s) 2737
XspI CTAG 6 cut(s) 473, 614, 1931, 2747, 2945, 3734
ZraI GACGTC 2 cut(s) 1167, 3654
Zsp2I ATGCAT 1 cut(s) 2208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.