MD11G1031300.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Forward (+)
2731712 .. 2747506
15795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1031300.v1.1.491

Sequence Viewer

Length: 4074 bp
ATGGTACTGGGAGAGGTTTTTCTTGGGGCGTTTCTACAGGTGCTGCTAGACAGGTTGACCCCTCGCGAGGTTCTCAACTACTTTGGAAACTTCCGGGGCCTTAGAAAGAAGCTGGAGAAGTGGAGGGCCACGTTATCTACAATCGCAGCGGTGTTGAGCGACGCTGAGGAAAGACAAGTGACTGACCATCCTGTAAAACTGTTGCTTGATGATCTCAGAGACCTCGCTTATGATGTCGAAGACATGTTGGACGAATTTTCCACTCAAATGTTGAAGCGCACAATAGAGGGACGTGATCAAGCTAGCACGAGCAACAAGGTGCGGAACTCATTTTTCAAAATTAAATTCAATTGGGATATGAGCTCCGAAATGAAGAAGATTACGGAGCGGTTGCAAGACATATCTGAACGGAAAGATAAGTTTGGCTTGAAAGATACTGGGACGTCTGCTAAGGAATCGCGACGTCTACCTAGTTCAGACGTGTTAGATGAAAAGCTTGTTGTTGGAAGAGATGGTGACAAAGGGAAGATTATTGAATTGTTGTCAAGAAAATACGAGCATGCAGGTGCTGTCAATTTTGATGTAGTTGCTATAGTTGGCATGGCCGGAGTTGGGAAGACGACACTTGCTCAACTTGTATTCAACAACAAAGATGATGCCATGAAGGAGTTTGAGCTCAAGGTCTGGGTATCTGTGTCCGATGACTTCGATGTGGTGCGAGTGACAAAGGCAATTCTTGAATCAATCACATCCCGACCCGTTAAAGTGCAGGAGTTTAGTAAAATGCAGCATAATTTGAGTGAGCAATTAAGAGGAAAAAAGTTTTTAATCGTTTTAGATGATATTTGGAACAAAGGCGACTATGATCTATACGATCTCTGGACAAGACTTCAATCCCCTTTTGGCGTCGGAGCAGGAGGAAGTAAGATAATTGTGACAACCCGTGACAGGAATGTTGCAAAGACTACGGGAGCCGCTGGAGTTCATAATTTGGAGTGTATGGCAGATGGTGATTGTTTGGAAATATTTGAGCGACATGCGTTCGGGGAAATTAATAATGGAAAGCCGGTAAATTATGATTTAATTGGGACAAAAATTGTTGAAACATGTCATGGATTACCATTAGCTGCGAGGACTCTCGGTGGTCTTCTACGTTGCAAAGAAAAAGATGAGTGGGGAGAAATATTGAACAACAAGTTATGGAATCCAGCAGATAAGAGTGGCATTCTCCCCGTACTAAAGTTGAGCTATCACTATCTTCCATCACATTTGAAGAGGTGTTTTGCCTATTGCTCCATTCTTCCAAATGACTATGAATTTGGGGAGAAACAGCTCGTCCTTTTGTGGATGGCAGAGGGTTTGATTCAACAAAAACCTGACGACAATAAACAAATGGAGGATTTGGGCCGCGACTACTTTCGAGAGCTATTAGCAAGGTCGCTGCTTCAAGGATCAAGCAAAAACAATTCACGATATGTAATGCATGACCTCGTTAATGATTTAGCACAATGGGCAGCAGGAGAAATATGTTTTAGGTTGGAAGATAAGCAAGGTGATAACTTGCAAAGCACTTGCTTTCAAAGGGCTCGCTATTCGTCTTTCATTGCTGGTGATTTTGATGGAGTTATGAGATTTGAGGCCTTTCCAAAACTTGAACGCTTGCGAACATTCCTGCCACTTTCGCTTTCAGATTCCAGGTGGTGGTGGAAATATTTGTCTCGTAAGGTTATTTTCGAGTTATTACCACAGATGAAATACTTACGAGTGCTCTCTTTCAATGGCTATAAAATAACTGAGCTGCCAAAGTCAATCGGTGATCTGAGGTTGTTACAGTATCTTGACCTTTCCTACACAGATATAACCTGTTTGCCTAAATCAACAAGCACTCTTTACAACTTGCAAACATTGATATTGGAGGGTTGTTCTAAATTGAAGTCATTGCCCACAAACATGAGTAATCTAATTAATTTGCGCCATCTCAACAACTCAGGTGCATCTTCATTGGTAGGAATGCCTTCACAACTAGGTCGATTGACGAATCTACAATCATTGCCTCTTTTTGTGGTGAGCAAAGGTAGTGATCAACCAGGGGTAAGAGAGATAGGGCCCCTATTGCATCTCCGAGGGACACTGTGCCTCTCAAGATTGGAGAATGTGACTGATGTCGAGGATGCCAGGAGGGCCAACTTGATATGCAAGGAGAGGCTTGATTCGTTGGTCCTAAAATGGTCTCATTCAAGCGACACGAGAAAAACAGAAACCGTTGTGCTTGACATGTTACAGCCTCATACAAAGCTCAAGGAGTTCACTCTCAAGAGTTATGCCGGAAAGGAATTTTCATCATGGGTTGGAGGTTCTTTCTTCTCTAATATGGTGCTTGTGCGCTTAGAGGAATGTAACAATTGTTTATCGTTGCCACCTCTCGGACAATTGCCTCGTCTCAAAGAGCTTTATATTAGAGAAATGAATGCAGTCGAAAGTGTTGGTGCTGAGTTTTATGGTGAGTGTGTCTTGCCCTTTCCGCTGTTAGAGATTCTGGAGTTTGTGGATATGCGGCATTGGAAGGTGTGGCTTCCTTTCCAACTGGATCAAGGAAGTAGTGTTTTCCCTTGCCTGAAAAGGCTTTCAATCAAAGGATGTTCTAAGTTGGAAGGCAAGCTGCCAGAGAAGCTCGATTTGTTAGCCGAACTTGAAATTGTTAAATGTGAGGAATTGGTGGTTTCGATTGCCAATTGCAAACAACTTCGTCAAATAAACATTGACAGTTGTAAAGTGTTGGTACATACAGATGCTAAGGTTGAGTTTGAGTTGTTAGAGTCCTTGTGCCTTTCAAACATTTCGGAGGTGATGTCTCTGCAAACAGGGGAATTGTTCAGGAAGGGATTAAGCAAGGCTAGAGATTTGAAGATTAATGGATGTGAGAAGCTGACGTCGTCATTGAAGAATGAGGGTAGATTATTGCAGCAGTTGACTTGTCTTGGCCGTTTGGAAATTGAAGACAACTCTCCTCTAGTTGAAGAATTGGGAGAAGAAGCAGAGGAGTTGCTGCAATTGCAAATATTGGAGTTAAAAAAGTGCGAAAATCTTTTGAAGCTACCAAAAGGATTAAATGAGCTGTCGTCTCTTCAAGAGCTTCGCATACACAAATGTTCACGTCTAGTTTCTTTTCCAGAAGTTGGTCTGCCACCGTCTCTTAAAAACATCGAGATTAGAGAGTGCCATTCGTTGATATATTTTGCAAAATTCCAGATTCCCCAATTTCTCAGAAGAATAGAGATAAGTAATTGCAGAAGTTTGAAATCACTAGTAGAACCGAACGGGTTATTCAGCGACAACACCAATCACTGCCTTGAATATATTAGGATCTGGAATTGCCAAAATCTGGAATCCTTACCGGATGGCTTATGCCACCTTAGCAATCTTCAAACTCTAATTATTGGGAACTGTGGAAGTCTTGTTTCCATCCCGAGACTGAGTGCGGAGAGAAGACCCTCCAACCTGAGAGATATCATGATCAACGAATGCGAGAAATTGGAGGCGTTTCCCGAAGACATGCACAATCTCAACTCTCTTGAGGGATTGAAGATCGACTACCGTAAAGGTTTGACTTTTCCTCCCAACCTAACATCACTTGAAATTTGGAAGGTCAAGAGCTGTAAGTCATTGTGGGAGTTGGAGTGGGGGTTGCACAGACTCACCTCTCTTAGAAAGTTGGGGATCAGTGGAAAAAACACACGTACGGTGTCGTTTCCACCTGACATAGTCCGGATGGAGCCGCTCCTCCCCAAATCTCTCACTGAACTCAGAATAGAAGGCTTCCCGAATCTGAAGAGACTGAGCAGCAAGGGCTTTCAATTCCTCAACTCCCTTCAATCTCTGCAACTCTCTAGATGTCCAAAGCTAGCATTCATTCCAGAGGAGGGTCTGCCTCCTTCACTTGGTCAGCTTATCATCTATAAGTGTCCTGTGCTAAAAGTGAGATGCCAACCAGGAAAAGGACTCTACTGGCACAAAATATCCAACATCCCTAAAATAGAGATACATGAAGCTAATGGTCCAGGTTTTGGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1358

Amino Acids

154.16

Weight (kDa)

8.15

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 102 3.1e-22 Rx N-terminal domain
NB-ARC PF00931 173 - 348 2.3e-35 NB-ARC domain
WHD_DRP PF23559 433 - 502 8.4e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 580 - 858 7.6e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 700 - 822 9.3e-35 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 1064 - 1176 3e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 554
AatII GACGTC 3 cut(s) 446, 466, 2933
Acc36I ACCTGC 1 cut(s) 554
AccB7I CCANNNNNTGG 4 cut(s) 1701, 3176, 3382, 4064
AccBSI CCGCTC 2 cut(s) 388, 3778
AccI GTMKAC 1 cut(s) 466
AccII CGCG 3 cut(s) 66, 460, 1410
AccIII TCCGGA 1 cut(s) 3765
AciI CCGC 9 cut(s) 149, 322, 388, 975, 1408, 2522, 2554, 3479, 3776
AclWI GGATC 4 cut(s) 1459, 2595, 3371, 3725
AcoI YGGCCR 2 cut(s) 603, 2980
AcsI RAATTY 6 cut(s) 254, 344, 1316, 2333, 3242, 3636
AcuI CTGAAG 1 cut(s) 3848
AcyI GRCGYC 4 cut(s) 443, 463, 906, 2930
AfaI GTAC 4 cut(s) 6, 1236, 2781, 3739
AflIII ACRYGT 5 cut(s) 243, 480, 1106, 2274, 3734
AhlI ACTAGT 1 cut(s) 3304
AjiI CACGTC 3 cut(s) 293, 481, 3155
AjnI CCWGG 5 cut(s) 1694, 2086, 2174, 3988, 4057
Alw21I GWGCWC 3 cut(s) 365, 678, 1770
Alw26I GTCTC 9 cut(s) 213, 1722, 2235, 2444, 2856, 3126, 3195, 3463, 3826
AlwI GGATC 4 cut(s) 1459, 2595, 3371, 3725
AlwNI CAGNNNCTG 2 cut(s) 43, 569
Ama87I CYCGRG 1 cut(s) 3466
Aor13HI TCCGGA 1 cut(s) 3765
AoxI GGCC 8 cut(s) 97, 126, 603, 1405, 1638, 2105, 2181, 2980
ApaI GGGCCC 1 cut(s) 2109
ApoI RAATTY 6 cut(s) 254, 344, 1316, 2333, 3242, 3636
ArsI GACNNNNNNTTYG 2 cut(s) 1301, 1333
AseI ATTAAT 3 cut(s) 1053, 1965, 2910
Asp700I GAANNNNTTC 1 cut(s) 2014
AspLEI GCGC 3 cut(s) 279, 1974, 2385
AspS9I GGNCC 8 cut(s) 97, 126, 1405, 2105, 2106, 2181, 2218, 4055
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 9 cut(s) 527, 1022, 1565, 1622, 1826, 2077, 2513, 2857, 3688
AsuNHI GCTAGC 2 cut(s) 302, 3901
AvaI CYCGRG 1 cut(s) 3466
AvaII GGWCC 2 cut(s) 2218, 4055
BaeGI GKGCMC 1 cut(s) 2109
BanII GRGCYC 4 cut(s) 365, 678, 1588, 2109
BarI GAAGNNNNNNTAC 2 cut(s) 3575, 3607
BauI CACGAG 2 cut(s) 307, 2245
BbsI GAAGAC 6 cut(s) 246, 623, 1139, 3003, 3493, 3555
Bbv12I GWGCWC 3 cut(s) 365, 678, 1770
BbvCI CCTCAGC 1 cut(s) 165
BceAI ACGGC 1 cut(s) 2967
BciT130I CCWGG 5 cut(s) 1696, 2088, 2176, 3990, 4059
BclI TGATCA 3 cut(s) 295, 2080, 3513
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 9 cut(s) 213, 1722, 2235, 2444, 2856, 3126, 3195, 3463, 3826
BcuI ACTAGT 1 cut(s) 3304
BfmI CTRYAG 2 cut(s) 35, 591
BfuAI ACCTGC 1 cut(s) 554
BglI GCCNNNNNGGC 1 cut(s) 2180
Bme1390I CCNGG 6 cut(s) 95, 1696, 2088, 2176, 3990, 4059
Bme18I GGWCC 2 cut(s) 2218, 4055
BmeT110I CYCGRG 1 cut(s) 3466
BmgBI CACGTC 3 cut(s) 293, 481, 3155
BmgT120I GGNCC 8 cut(s) 97, 126, 1405, 2105, 2106, 2181, 2218, 4055
BmiI GGNNCC 5 cut(s) 98, 973, 2107, 2108, 3774
BmrFI CCNGG 6 cut(s) 95, 1696, 2088, 2176, 3990, 4059
BmrI ACTGGG 2 cut(s) 17, 447
BmsI GCATC 6 cut(s) 646, 2003, 2125, 2161, 2779, 3971
BmtI GCTAGC 2 cut(s) 306, 3905
BmuI ACTGGG 2 cut(s) 17, 447
BoxI GACNNNNGTC 1 cut(s) 2162
BpiI GAAGAC 6 cut(s) 246, 623, 1139, 3003, 3493, 3555
BpmI CTGGAG 3 cut(s) 134, 999, 2558
Bpu10I CCTNAGC 4 cut(s) 165, 450, 2793, 3413
BpuEI CTTGAG 5 cut(s) 662, 2125, 2282, 2297, 3593
BpuMI CCSGG 1 cut(s) 95
BsaAI YACGTR 1 cut(s) 3737
BsaHI GRCGYC 4 cut(s) 443, 463, 906, 2930
BsaI GGTCTC 2 cut(s) 213, 2235
BsaJI CCNNGG 3 cut(s) 94, 2087, 2122
BsaWI WCCGGW 2 cut(s) 3394, 3765
BsaXI ACNNNNNCTCC 8 cut(s) 2834, 2864, 3598, 3628, 3662, 3668, 3692, 3698
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 4 cut(s) 12, 442, 2589, 4010
Bse3DI GCAATG 3 cut(s) 1602, 1937, 2048
BseAI TCCGGA 1 cut(s) 3765
BseBI CCWGG 5 cut(s) 1696, 2088, 2176, 3990, 4059
BseDI CCNNGG 3 cut(s) 94, 2087, 2122
BseMI GCAATG 3 cut(s) 1602, 1937, 2048
BseNI ACTGG 4 cut(s) 12, 442, 2589, 4010
BseRI GAGGAG 4 cut(s) 2997, 3053, 3770, 3932
BseSI GKGCMC 1 cut(s) 2109
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 3 cut(s) 66, 460, 1410
BshFI GGCC 8 cut(s) 99, 128, 605, 1407, 1640, 2107, 2183, 2982
BsiHKAI GWGCWC 3 cut(s) 365, 678, 1770
BsiHKCI CYCGRG 1 cut(s) 3466
BsiSI CCGG 6 cut(s) 94, 606, 1067, 2325, 3395, 3766
BsiWI CGTACG 1 cut(s) 3737
BslFI GGGAC 4 cut(s) 303, 454, 1102, 2140
BsmAI GTCTC 9 cut(s) 213, 1722, 2235, 2444, 2856, 3126, 3195, 3463, 3826
BsmBI CGTCTC 3 cut(s) 2444, 3126, 3195
BsmFI GGGAC 4 cut(s) 303, 454, 1102, 2140
BsmI GAATGC 5 cut(s) 1224, 2016, 2473, 3527, 3905
BsnI GGCC 8 cut(s) 99, 128, 605, 1407, 1640, 2107, 2183, 2982
Bso31I GGTCTC 2 cut(s) 213, 2235
BsoBI CYCGRG 1 cut(s) 3466
Bsp120I GGGCCC 1 cut(s) 2105
Bsp1286I GDGCHC 5 cut(s) 365, 678, 1588, 1770, 2109
Bsp13I TCCGGA 1 cut(s) 3765
Bsp68I TCGCGA 2 cut(s) 66, 460
BspACI CCGC 9 cut(s) 149, 322, 388, 975, 1408, 2522, 2554, 3479, 3776
BspANI GGCC 8 cut(s) 99, 128, 605, 1407, 1640, 2107, 2183, 2982
BspEI TCCGGA 1 cut(s) 3765
BspFNI CGCG 3 cut(s) 66, 460, 1410
BspHI TCATGA 1 cut(s) 3510
BspLI GGNNCC 5 cut(s) 98, 973, 2107, 2108, 3774
BspMI ACCTGC 1 cut(s) 554
BspOI GCTAGC 2 cut(s) 306, 3905
BspPI GGATC 4 cut(s) 1459, 2595, 3371, 3725
BspTNI GGTCTC 2 cut(s) 213, 2235
BsrBI CCGCTC 2 cut(s) 388, 3778
BsrDI GCAATG 3 cut(s) 1602, 1937, 2048
BsrFI RCCGGY 1 cut(s) 1066
BsrI ACTGG 4 cut(s) 12, 442, 2589, 4010
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 3 cut(s) 94, 2087, 2122
BssNI GRCGYC 4 cut(s) 443, 463, 906, 2930
BssSI CACGAG 2 cut(s) 307, 2245
Bst2BI CACGAG 2 cut(s) 307, 2245
Bst2UI CCWGG 5 cut(s) 1696, 2088, 2176, 3990, 4059
Bst4CI ACNGT 9 cut(s) 201, 1833, 2133, 2263, 2765, 3189, 3446, 3596, 3742
Bst6I CTCTTC 4 cut(s) 502, 1268, 3129, 3824
BstACI GRCGYC 4 cut(s) 443, 463, 906, 2930
BstBAI YACGTR 1 cut(s) 3737
BstC8I GCNNGC 6 cut(s) 304, 561, 1588, 1661, 2657, 3903
BstFNI CGCG 3 cut(s) 66, 460, 1410
BstHHI GCGC 3 cut(s) 279, 1974, 2385
BstMAI GTCTC 9 cut(s) 213, 1722, 2235, 2444, 2856, 3126, 3195, 3463, 3826
BstMWI GCNNNNNNNGC 9 cut(s) 1511, 1681, 2113, 2180, 2521, 2668, 3052, 3414, 3846
BstNI CCWGG 5 cut(s) 1696, 2088, 2176, 3990, 4059
BstNSI RCATGY 6 cut(s) 247, 563, 1040, 1110, 2278, 3556
BstPAI GACNNNNGTC 1 cut(s) 2162
BstSCI CCNGG 6 cut(s) 93, 1694, 2086, 2174, 3988, 4057
BstSFI CTRYAG 2 cut(s) 35, 591
BstSLI GKGCMC 1 cut(s) 2109
BstUI CGCG 3 cut(s) 66, 460, 1410
BstV2I GAAGAC 6 cut(s) 246, 623, 1139, 3003, 3493, 3555
BstX2I RGATCY 1 cut(s) 3363
BstYI RGATCY 1 cut(s) 3363
BsuRI GGCC 8 cut(s) 99, 128, 605, 1407, 1640, 2107, 2183, 2982
BtrI CACGTC 3 cut(s) 293, 481, 3155
BtsI GCAGTG 1 cut(s) 3343
BtsIMutI CAGTG 4 cut(s) 2129, 3343, 3727, 3795
BtuMI TCGCGA 2 cut(s) 66, 460
BveI ACCTGC 1 cut(s) 554
Cac8I GCNNGC 6 cut(s) 304, 561, 1588, 1661, 2657, 3903
CaiI CAGNNNCTG 2 cut(s) 43, 569
CciI TCATGA 1 cut(s) 3510
CfoI GCGC 3 cut(s) 279, 1974, 2385
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 8 cut(s) 97, 126, 1405, 2105, 2106, 2181, 2218, 4055
CseI GACGC 2 cut(s) 170, 895
Csp6I GTAC 4 cut(s) 5, 1235, 2780, 3738
CviQI GTAC 4 cut(s) 5, 1235, 2780, 3738
EaeI YGGCCR 2 cut(s) 603, 2980
Eam1104I CTCTTC 4 cut(s) 502, 1268, 3129, 3824
EarI CTCTTC 4 cut(s) 502, 1268, 3129, 3824
Ecl136II GAGCTC 2 cut(s) 363, 676
Eco147I AGGCCT 1 cut(s) 1640
Eco24I GRGCYC 4 cut(s) 365, 678, 1588, 2109
Eco31I GGTCTC 2 cut(s) 213, 2235
Eco32I GATATC 1 cut(s) 3508
Eco47I GGWCC 2 cut(s) 2218, 4055
Eco53kI GAGCTC 2 cut(s) 363, 676
Eco57I CTGAAG 1 cut(s) 3848
Eco88I CYCGRG 1 cut(s) 3466
EcoICRI GAGCTC 2 cut(s) 363, 676
EcoO109I RGGNCCY 3 cut(s) 97, 2105, 2106
EcoRII CCWGG 5 cut(s) 1694, 2086, 2174, 3988, 4057
EcoRV GATATC 1 cut(s) 3508
EcoT22I ATGCAT 1 cut(s) 1485
EcoT38I GRGCYC 4 cut(s) 365, 678, 1588, 2109
Esp3I CGTCTC 3 cut(s) 2444, 3126, 3195
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 4 cut(s) 303, 454, 1102, 2140
FbaI TGATCA 3 cut(s) 295, 2080, 3513
FblI GTMKAC 1 cut(s) 466
FriOI GRGCYC 4 cut(s) 365, 678, 1588, 2109
GlaI GCGC 3 cut(s) 278, 1973, 2384
GsuI CTGGAG 3 cut(s) 134, 999, 2558
HaeIII GGCC 8 cut(s) 99, 128, 605, 1407, 1640, 2107, 2183, 2982
HapII CCGG 6 cut(s) 94, 606, 1067, 2325, 3395, 3766
HgaI GACGC 2 cut(s) 170, 895
HhaI GCGC 3 cut(s) 279, 1974, 2385
Hin1I GRCGYC 4 cut(s) 443, 463, 906, 2930
Hin6I GCGC 3 cut(s) 277, 1972, 2383
HinP1I GCGC 3 cut(s) 277, 1972, 2383
HincII GTYRAC 2 cut(s) 57, 2970
HindII GTYRAC 2 cut(s) 57, 2970
HindIII AAGCTT 1 cut(s) 494
HpaII CCGG 6 cut(s) 94, 606, 1067, 2325, 3395, 3766
HphI GGTGA 9 cut(s) 527, 1022, 1565, 1622, 1826, 2077, 2513, 2857, 3688
Hpy166II GTNNAC 5 cut(s) 57, 467, 2307, 2970, 3152
Hpy8I GTNNAC 5 cut(s) 57, 467, 2307, 2970, 3152
Hpy99I CGWCG 4 cut(s) 164, 465, 911, 2935
HpyAV CCTTC 9 cut(s) 658, 2025, 2557, 2645, 2872, 3637, 3806, 3878, 3942
HpyCH4III ACNGT 9 cut(s) 201, 1833, 2133, 2263, 2765, 3189, 3446, 3596, 3742
HpyCH4IV ACGT 9 cut(s) 131, 292, 443, 463, 480, 1153, 2930, 3154, 3736
HpyF10VI GCNNNNNNNGC 9 cut(s) 1511, 1681, 2113, 2180, 2521, 2668, 3052, 3414, 3846
HpySE526I ACGT 9 cut(s) 131, 292, 443, 463, 480, 1153, 2930, 3154, 3736
Hsp92I GRCGYC 4 cut(s) 443, 463, 906, 2930
HspAI GCGC 3 cut(s) 277, 1972, 2383
Kpn2I TCCGGA 1 cut(s) 3765
Ksp22I TGATCA 3 cut(s) 295, 2080, 3513
LmnI GCTCC 7 cut(s) 368, 385, 911, 971, 1298, 3772, 3783
LweI GCATC 6 cut(s) 646, 2003, 2125, 2161, 2779, 3971
MaeII ACGT 9 cut(s) 131, 292, 443, 463, 480, 1153, 2930, 3154, 3736
MaeIII GTNAC 9 cut(s) 178, 515, 721, 934, 944, 1827, 2155, 2277, 2396
MbiI CCGCTC 2 cut(s) 388, 3778
MfeI CAATTG 5 cut(s) 349, 2401, 2429, 2731, 3050
MflI RGATCY 1 cut(s) 3363
MhlI GDGCHC 5 cut(s) 365, 678, 1588, 1770, 2109
MlyI GAGTC 4 cut(s) 1129, 2825, 3687, 3993
Mph1103I ATGCAT 1 cut(s) 1485
MroI TCCGGA 1 cut(s) 3765
MroXI GAANNNNTTC 1 cut(s) 2014
MslI CAYNNNNRTG 4 cut(s) 266, 564, 1949, 2787
MspA1I CMGCKG 3 cut(s) 149, 977, 2524
MspI CCGG 6 cut(s) 94, 606, 1067, 2325, 3395, 3766
MspR9I CCNGG 6 cut(s) 95, 1696, 2088, 2176, 3990, 4059
MunI CAATTG 5 cut(s) 349, 2401, 2429, 2731, 3050
Mva1269I GAATGC 5 cut(s) 1224, 2016, 2473, 3527, 3905
MvaI CCWGG 5 cut(s) 1696, 2088, 2176, 3990, 4059
MvnI CGCG 3 cut(s) 66, 460, 1410
MwoI GCNNNNNNNGC 9 cut(s) 1511, 1681, 2113, 2180, 2521, 2668, 3052, 3414, 3846
NciI CCSGG 1 cut(s) 95
NheI GCTAGC 2 cut(s) 302, 3901
NlaIV GGNNCC 5 cut(s) 98, 973, 2107, 2108, 3774
NmuCI GTSAC 6 cut(s) 178, 515, 721, 934, 944, 2155
NruI TCGCGA 2 cut(s) 66, 460
NsiI ATGCAT 1 cut(s) 1485
NspI RCATGY 6 cut(s) 247, 563, 1040, 1110, 2278, 3556
PaeI GCATGC 1 cut(s) 563
PagI TCATGA 1 cut(s) 3510
PaqCI CACCTGC 1 cut(s) 554
PceI AGGCCT 1 cut(s) 1640
PciI ACATGT 3 cut(s) 243, 1106, 2274
PctI GAATGC 5 cut(s) 1224, 2016, 2473, 3527, 3905
PdmI GAANNNNTTC 1 cut(s) 2014
Pfl23II CGTACG 1 cut(s) 3737
PflFI GACNNNGTC 2 cut(s) 2932, 3761
PflMI CCANNNNNTGG 4 cut(s) 1701, 3176, 3382, 4064
PleI GAGTC 4 cut(s) 1129, 2824, 3687, 3993
PpsI GAGTC 4 cut(s) 1129, 2824, 3687, 3993
Ppu21I YACGTR 1 cut(s) 3737
PscI ACATGT 3 cut(s) 243, 1106, 2274
PshAI GACNNNNGTC 1 cut(s) 2162
PshBI ATTAAT 3 cut(s) 1053, 1965, 2910
Psp124BI GAGCTC 2 cut(s) 365, 678
Psp6I CCWGG 5 cut(s) 1694, 2086, 2174, 3988, 4057
PspGI CCWGG 5 cut(s) 1694, 2086, 2174, 3988, 4057
PspLI CGTACG 1 cut(s) 3737
PspN4I GGNNCC 5 cut(s) 98, 973, 2107, 2108, 3774
PspOMI GGGCCC 1 cut(s) 2105
PspPI GGNCC 8 cut(s) 97, 126, 1405, 2105, 2106, 2181, 2218, 4055
PstNI CAGNNNCTG 2 cut(s) 43, 569
PsuI RGATCY 1 cut(s) 3363
PsyI GACNNNGTC 2 cut(s) 2932, 3761
RruI TCGCGA 2 cut(s) 66, 460
RsaI GTAC 4 cut(s) 6, 1236, 2781, 3739
RsaNI GTAC 4 cut(s) 5, 1235, 2780, 3738
RseI CAYNNNNRTG 4 cut(s) 266, 564, 1949, 2787
SacI GAGCTC 2 cut(s) 365, 678
Sau96I GGNCC 8 cut(s) 97, 126, 1405, 2105, 2106, 2181, 2218, 4055
SchI GAGTC 4 cut(s) 1129, 2825, 3687, 3993
ScrFI CCNGG 6 cut(s) 95, 1696, 2088, 2176, 3990, 4059
SduI GDGCHC 5 cut(s) 365, 678, 1588, 1770, 2109
SfaNI GCATC 6 cut(s) 646, 2003, 2125, 2161, 2779, 3971
SfcI CTRYAG 2 cut(s) 35, 591
SinI GGWCC 2 cut(s) 2218, 4055
SmiMI CAYNNNNRTG 4 cut(s) 266, 564, 1949, 2787
SmlI CTYRAG 5 cut(s) 677, 2140, 2297, 2312, 3572
SmoI CTYRAG 5 cut(s) 677, 2140, 2297, 2312, 3572
SpeI ACTAGT 1 cut(s) 3304
SphI GCATGC 1 cut(s) 563
SseBI AGGCCT 1 cut(s) 1640
SsiI CCGC 9 cut(s) 149, 322, 388, 975, 1408, 2522, 2554, 3479, 3776
SspI AATATT 4 cut(s) 1026, 1185, 1712, 3060
SstI GAGCTC 2 cut(s) 365, 678
StuI AGGCCT 1 cut(s) 1640
StyD4I CCNGG 6 cut(s) 93, 1694, 2086, 2174, 3988, 4057
TaaI ACNGT 9 cut(s) 201, 1833, 2133, 2263, 2765, 3189, 3446, 3596, 3742
TaiI ACGT 9 cut(s) 134, 295, 446, 466, 483, 1156, 2933, 3157, 3739
TauI GCSGC 4 cut(s) 977, 1410, 2557, 3778
TscAI CASTG 4 cut(s) 2136, 3350, 3727, 3802
TseFI GTSAC 6 cut(s) 178, 515, 721, 934, 944, 2155
Tsp45I GTSAC 6 cut(s) 178, 515, 721, 934, 944, 2155
TspGWI ACGGA 2 cut(s) 398, 424
TspRI CASTG 4 cut(s) 2136, 3350, 3727, 3802
Tth111I GACNNNGTC 2 cut(s) 2932, 3761
Van91I CCANNNNNTGG 4 cut(s) 1701, 3176, 3382, 4064
VpaK11BI GGWCC 2 cut(s) 2218, 4055
VspI ATTAAT 3 cut(s) 1053, 1965, 2910
XapI RAATTY 6 cut(s) 254, 344, 1316, 2333, 3242, 3636
XbaI TCTAGA 1 cut(s) 3887
XceI RCATGY 6 cut(s) 247, 563, 1040, 1110, 2278, 3556
XmiI GTMKAC 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 2014
ZraI GACGTC 3 cut(s) 444, 464, 2931
Zsp2I ATGCAT 1 cut(s) 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.