RchiOBHm_Chr1g0328061

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Forward (+)
17315902 .. 17318735
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55754

Sequence Viewer

Length: 2778 bp
ATGGTAGCCGAGCTCCTTCTTGCAGCATTCCTTCGGGTGCTGCTTCAGAAATTGACCCTTCGCGCCTTCATCGCCTCTTTAGGAAAAGAGGGAATCGGAAAAAAGGTGAACAAATGGGAGAAAACATTGTCTGCAATTGAAGCGGTCTTGTGTGATGCGGAAGAGAAGCAACAAACAAGCAGGGCTGTGAAACTATGGTTGGATGATCTCAAAGACTTGGCTTATGATGTGGAGGACATCCTGGACAAGTTTTACACTGAAATGTTACGACGCAAAGTGAATAAGCCAAATCAGGCTGGAGTCTGTAAGGTACGGGGCCTCTTTCCTAGACTTAAATTGCACTTCAATATGAAGCATGAAATACAGGAAATGACTGATCGATTAGATGAAATATCTGAACGAAAAGACAGGCTCGGTTTAATAGATATTGGGGATATTCCAGACAAGAGTCGGCCACCAAGTTCACGTGTGTTAGATGGCGTGCCTGTTGTTGGAAGAGACAGTGATAGAGGGAAGATTGTTGAATTTCTTTTAAGAAACAACCCTAATGATGCCAACTTTGAGGTAGCTGCCATTGTTGGCATGCCTGGGATTGGGAAGACAACACTTGCTCAGTATGTAATTAATGATAACAGTGACATCCTCAAAGAGTTCGATCTTAAGATATGGGTATGTGTGTCTAATGACTTCAAAGTCTTGAGGGTAACAAAAGCAATCCTAGAATCAGTCACGTCTAAGCATTGTGATTTGGACCAATTCAGTAACATCCAGAATCATCTGTGTAAAGTATTAACAGGTAAGAAGTTCTTACTTGTTTTAGATGATGTCTGGAGCACCTGTAACTATGATCAGTGGAAGATGCTGCAATCCCCCTTTCATGTTGGAGCATCGGGAAGTAAGATAATTGTAACGACACGGGATGTGGAAGTTACCAAGATGATGAGAGCCGCTGAAGTTCACAGTTTGATGGGTATATCAAATGATGATTGTTGGAAAGTGTTTGAGCAACATGCCTATTTTAATGTTGACAATGGCATACCACAAAATTTCAGGTTACTTCAGGAGAAAATAATTGCAAAATGTGGTGGATTACCCCTGGCAGCGAGGACTCTTGGTGGCCTTTTTGGTTGTAAGGAAATAACTGAATGGGAAAAAATGTTGGACAACAAATTATGGCTTTCAGATAAGAGTGACATTCTTCCAGTTTTACAATTGAGTTATTATTATCTCCCGTCAAAATTGAAAAGATGCTTTGCCTATTGCTCAATACTTCCAAAGGACTATGAATTTGGGGAGACACAGTTGATCCTTCTATGGATGGCAGAGGGCTTGATTGAGAAACCACTCAAACCAGAAGATAGTAGACATTTGGAAGATGTAGCGAGTGAATATTTCAGTGAGCTACTATCTAGGTCATTGTTTCAAAAATCGAGCAAAAAAAATTCAAGATACGTAATGCATAACCTTGTTAGTGATATGGCACATAAGGTTGCAGGAGAAATTTGTTTTCGATTGGAGGATAAGCTGGATGGTAGCTGTTCTCCAAAGTCTCGTCATTTGTCTTACATTACTGGGAGGTATGATGGGGTTAAAAGATTTGGTGCTGTTTCTGAGGTACACCGTTTGCGGACTTTCCTACCACTTACAATTTCAGATGACCCATGCAATTACTTGACTAGTAAGGTTACTCTTGGCTTACTACCAAAGTTGCAATACTTGCGGGTGCTCTCTTTGAATGGTTATCGTATAAGTCAGCTGCCAAACTCCATTGGTGAATTGAAGTTTCTGCGGTACCTGGATCTTTCTCACACAGAAATAACAAGTTTGCCTGAATCAGCAAGCACACTTTACAATTTGCAGACGTTGATATTAGAGAGTTGTTATAGTTTGAAGGCACTACCTACAAACATGAAGAATCTAATTAATCTTCGTCATCTGAGCAATTCTAATACACCTCTGTTGGAAGGAATGCCTGCACAACTAGGTCAGTTGACTAATTTGCAAACACTGTGCAATTTTGTAGTGGGCAGAGGAAGCAATTCGGGGATAAGAGAGATAGAGCCCCTATTGCATCTTCGAGGAACATTGCGCCTTTCAAGATTGGAGAATGTGAATGACATTGAGGATGCAAAGAGGGCCGACTTTGTTAGCAAGGAGGGTCTTGAGGCTTTTCTACTAGAATGGGGTGGCCTAGGAGAAAAAGAACTAGAGCTGCTCAACATGTTAGAGCCTCATAGAAAGCTCAAAGTTCTTAGTATCAAGGGCTACGGTGGTTTGGAATTTTCAACATGGATGGGACATCATTTATTCTCTAATATGACAGTTGTAAGGTTGGAGGATTGTAAAAACTGTGGTTTGTTGCCACCACTTGGTCAGTTGCCTTCTCTTAGAAAGCTTATTATAAAAAGAATGTATAGGGTGGAAAGTGTTGGTCCAGAGTTTTATGGAAATGATAACTTGCCCTTTCCACTGTTAGAGATCCTTGAGTTTGAGGAGATGAAAGATTGGAAAGAGTGGGTTTCACTTGAACGGGATCAAGGAATTGGAGCTTTCCCTTGCCTAAAAATGCTTTCCATTTCCAGATGTCCAAAGTTGGAGGGTAGGTTACCCAAGAATCTTGCTTTATTATCAGAGCTTGTGATTCGTGGATGCGAGCAGTTGGTTGTTTCCATTGCCAACTATAAACAACTTCAGAGATTTGATATTTGTGATTGCAAAGGGGTGGTCGATGGAAGTACTCCATACAAAGGACTTTCTGAGAGGATTAAAGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

925

Amino Acids

105.04

Weight (kDa)

8.15

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 95 5.9e-21 Rx N-terminal domain
NB-ARC PF00931 171 - 339 2.5e-38 NB-ARC domain
WHD_DRP PF23559 423 - 494 1.3e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 557 - 834 5.2e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 683 - 804 1.3e-29 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2402
AasI GACNNNNNNGTC 1 cut(s) 692
Acc65I GGTACC 1 cut(s) 1791
AccB1I GGYRCC 1 cut(s) 1791
AccB7I CCANNNNNTGG 1 cut(s) 2369
AccI GTMKAC 1 cut(s) 1363
AccII CGCG 1 cut(s) 63
AciI CCGC 6 cut(s) 143, 158, 948, 1627, 1720, 1789
AclWI GGATC 4 cut(s) 1300, 1806, 2473, 2541
AcoI YGGCCR 1 cut(s) 452
AcsI RAATTY 6 cut(s) 524, 1045, 1286, 1441, 1500, 2279
AcuI CTGAAG 4 cut(s) 29, 972, 1043, 2675
AcvI CACGTG 1 cut(s) 467
AfaI GTAC 4 cut(s) 312, 1617, 1793, 2737
AfiI CCNNNNNNNGG 4 cut(s) 491, 593, 2369, 2747
AflII CTTAAG 1 cut(s) 659
AflIII ACRYGT 2 cut(s) 466, 2220
AhlI ACTAGT 1 cut(s) 1676
AjiI CACGTC 1 cut(s) 732
AjnI CCWGG 4 cut(s) 240, 586, 1095, 1794
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
Alw21I GWGCWC 3 cut(s) 15, 836, 1728
Alw26I GTCTC 3 cut(s) 492, 1289, 1554
AlwI GGATC 4 cut(s) 1300, 1806, 2473, 2541
AoxI GGCC 5 cut(s) 316, 452, 1117, 2136, 2188
ApeKI GCWGC 7 cut(s) 23, 40, 569, 862, 1100, 1756, 2212
ApoI RAATTY 6 cut(s) 524, 1045, 1286, 1441, 1500, 2279
ArsI GACNNNNNNTTYG 4 cut(s) 1271, 1303, 2709, 2741
AseI ATTAAT 2 cut(s) 624, 1923
Asp700I GAANNNNTTC 1 cut(s) 2038
Asp718I GGTACC 1 cut(s) 1791
AspA2I CCTAGG 1 cut(s) 2191
AspLEI GCGC 2 cut(s) 65, 2091
AspS9I GGNCC 4 cut(s) 316, 751, 2136, 2432
AsuHPI GGTGA 2 cut(s) 118, 1784
AvaII GGWCC 2 cut(s) 751, 2432
AvrII CCTAGG 1 cut(s) 2191
BanI GGYRCC 1 cut(s) 1791
BanII GRGCYC 2 cut(s) 15, 2064
BbrPI CACGTG 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 3 cut(s) 15, 836, 1728
BbvI GCAGC 7 cut(s) 27, 35, 556, 849, 1112, 1743, 2199
BccI CCATC 7 cut(s) 470, 961, 1312, 1523, 1577, 2287, 2723
BciT130I CCWGG 4 cut(s) 242, 588, 1097, 1796
BclI TGATCA 1 cut(s) 847
BcoDI GTCTC 3 cut(s) 492, 1289, 1554
BcuI ACTAGT 1 cut(s) 1676
BfaI CTAG 8 cut(s) 327, 719, 1410, 1677, 1982, 2177, 2192, 2207
BfrI CTTAAG 1 cut(s) 659
BisI GCNGC 8 cut(s) 24, 41, 570, 863, 948, 1101, 1757, 2213
BlnI CCTAGG 1 cut(s) 2191
BlsI GCNGC 8 cut(s) 25, 42, 571, 864, 949, 1102, 1758, 2214
BmcAI AGTACT 1 cut(s) 2737
Bme1390I CCNGG 4 cut(s) 242, 588, 1097, 1796
Bme18I GGWCC 2 cut(s) 751, 2432
BmgBI CACGTC 1 cut(s) 732
BmgT120I GGNCC 4 cut(s) 316, 751, 2136, 2432
BmiI GGNNCC 2 cut(s) 317, 1793
BmrFI CCNGG 4 cut(s) 242, 588, 1097, 1796
BmrI ACTGGG 1 cut(s) 1581
BmsI GCATC 8 cut(s) 145, 541, 849, 896, 1238, 2080, 2116, 2639
BmuI ACTGGG 1 cut(s) 1581
BoxI GACNNNNGTC 1 cut(s) 447
BpiI GAAGAC 1 cut(s) 605
BpmI CTGGAG 2 cut(s) 318, 850
BpuEI CTTGAG 3 cut(s) 718, 2183, 2504
Bsa29I ATCGAT 1 cut(s) 379
BsaAI YACGTR 2 cut(s) 467, 1453
BsaJI CCNNGG 3 cut(s) 587, 1095, 2191
BsaXI ACNNNNNCTCC 6 cut(s) 823, 853, 1286, 1316, 2588, 2618
Bsc4I CCNNNNNNNGG 4 cut(s) 491, 593, 2369, 2747
Bse1I ACTGG 2 cut(s) 1202, 1576
Bse3DI GCAATG 2 cut(s) 2084, 2670
BseBI CCWGG 4 cut(s) 242, 588, 1097, 1796
BseCI ATCGAT 1 cut(s) 379
BseDI CCNNGG 3 cut(s) 587, 1095, 2191
BseLI CCNNNNNNNGG 4 cut(s) 491, 593, 2369, 2747
BseMI GCAATG 2 cut(s) 2084, 2670
BseMII CTCAG 4 cut(s) 626, 1602, 1928, 2748
BseNI ACTGG 2 cut(s) 1202, 1576
BseRI GAGGAG 1 cut(s) 2507
BseXI GCAGC 7 cut(s) 27, 35, 556, 849, 1112, 1743, 2199
BsgI GTGCAG 1 cut(s) 1959
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 5 cut(s) 318, 454, 1119, 2138, 2190
BshNI GGYRCC 1 cut(s) 1791
BshVI ATCGAT 1 cut(s) 379
BsiHKAI GWGCWC 3 cut(s) 15, 836, 1728
BslFI GGGAC 1 cut(s) 2310
BslI CCNNNNNNNGG 4 cut(s) 491, 593, 2369, 2747
BsmAI GTCTC 3 cut(s) 492, 1289, 1554
BsmFI GGGAC 1 cut(s) 2310
BsmI GAATGC 2 cut(s) 26, 1974
BsnI GGCC 5 cut(s) 318, 454, 1119, 2138, 2190
Bsp1286I GDGCHC 4 cut(s) 15, 836, 1728, 2064
Bsp143I GATC 8 cut(s) 205, 376, 655, 847, 1305, 1798, 2478, 2533
BspACI CCGC 6 cut(s) 143, 158, 948, 1627, 1720, 1789
BspANI GGCC 5 cut(s) 318, 454, 1119, 2138, 2190
BspCNI CTCAG 4 cut(s) 625, 1603, 1929, 2749
BspDI ATCGAT 1 cut(s) 379
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 317, 1793
BspPI GGATC 4 cut(s) 1300, 1806, 2473, 2541
BspT107I GGYRCC 1 cut(s) 1791
BspTI CTTAAG 1 cut(s) 659
BsrDI GCAATG 2 cut(s) 2084, 2670
BsrI ACTGG 2 cut(s) 1202, 1576
BssECI CCNNGG 3 cut(s) 587, 1095, 2191
BssMI GATC 8 cut(s) 205, 376, 655, 847, 1305, 1798, 2478, 2533
BssT1I CCWWGG 1 cut(s) 2191
Bst2UI CCWGG 4 cut(s) 242, 588, 1097, 1796
Bst6I CTCTTC 2 cut(s) 156, 490
BstAFI CTTAAG 1 cut(s) 659
BstAPI GCANNNNNTGC 1 cut(s) 1717
BstBAI YACGTR 2 cut(s) 467, 1453
BstC8I GCNNGC 5 cut(s) 482, 584, 1840, 1974, 2654
BstDEI CTNAG 7 cut(s) 612, 735, 1611, 1937, 2252, 2387, 2757
BstEII GGTNACC 1 cut(s) 2604
BstFNI CGCG 1 cut(s) 63
BstHHI GCGC 2 cut(s) 65, 2091
BstKTI GATC 8 cut(s) 208, 379, 658, 850, 1308, 1801, 2481, 2536
BstMAI GTCTC 3 cut(s) 492, 1289, 1554
BstMBI GATC 8 cut(s) 205, 376, 655, 847, 1305, 1798, 2478, 2533
BstMWI GCNNNNNNNGC 6 cut(s) 71, 140, 1717, 2034, 2068, 2135
BstNI CCWGG 4 cut(s) 242, 588, 1097, 1796
BstNSI RCATGY 3 cut(s) 586, 1013, 2224
BstPAI GACNNNNGTC 1 cut(s) 447
BstPI GGTNACC 1 cut(s) 2604
BstSCI CCNGG 4 cut(s) 240, 586, 1095, 1794
BstSNI TACGTA 1 cut(s) 1453
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 7 cut(s) 27, 35, 556, 849, 1112, 1743, 2199
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 1798, 2478
BstYI RGATCY 2 cut(s) 1798, 2478
Bsu15I ATCGAT 1 cut(s) 379
BsuRI GGCC 5 cut(s) 318, 454, 1119, 2138, 2190
BsuTUI ATCGAT 1 cut(s) 379
BtgZI GCGATG 1 cut(s) 55
BtrI CACGTC 1 cut(s) 732
BtsIMutI CAGTG 7 cut(s) 255, 508, 640, 857, 1402, 2006, 2468
Cac8I GCNNGC 5 cut(s) 482, 584, 1840, 1974, 2654
CfoI GCGC 2 cut(s) 65, 2091
Cfr13I GGNCC 4 cut(s) 316, 751, 2136, 2432
ClaI ATCGAT 1 cut(s) 379
CseI GACGC 1 cut(s) 279
Csp6I GTAC 4 cut(s) 311, 1616, 1792, 2736
CviAII CATG 8 cut(s) 356, 583, 878, 1010, 1662, 1909, 2221, 2289
CviQI GTAC 4 cut(s) 311, 1616, 1792, 2736
DdeI CTNAG 7 cut(s) 612, 735, 1611, 1937, 2252, 2387, 2757
DpnI GATC 8 cut(s) 207, 378, 657, 849, 1307, 1800, 2480, 2535
DpnII GATC 8 cut(s) 205, 376, 655, 847, 1305, 1798, 2478, 2533
DrdI GACNNNNNNGTC 1 cut(s) 692
DseDI GACNNNNNNGTC 1 cut(s) 692
EaeI YGGCCR 1 cut(s) 452
Eam1104I CTCTTC 2 cut(s) 156, 490
EarI CTCTTC 2 cut(s) 156, 490
Ecl136II GAGCTC 1 cut(s) 13
Eco105I TACGTA 1 cut(s) 1453
Eco130I CCWWGG 1 cut(s) 2191
Eco24I GRGCYC 2 cut(s) 15, 2064
Eco47I GGWCC 2 cut(s) 751, 2432
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 4 cut(s) 29, 972, 1043, 2675
Eco72I CACGTG 1 cut(s) 467
Eco91I GGTNACC 1 cut(s) 2604
EcoICRI GAGCTC 1 cut(s) 13
EcoO109I RGGNCCY 1 cut(s) 316
EcoO65I GGTNACC 1 cut(s) 2604
EcoRII CCWGG 4 cut(s) 240, 586, 1095, 1794
EcoT14I CCWWGG 1 cut(s) 2191
EcoT22I ATGCAT 1 cut(s) 1461
EcoT38I GRGCYC 2 cut(s) 15, 2064
ErhI CCWWGG 1 cut(s) 2191
FaeI CATG 8 cut(s) 359, 586, 881, 1013, 1665, 1912, 2224, 2292
FalI AAGNNNNNCTT 2 cut(s) 791, 823
FaqI GGGAC 1 cut(s) 2310
FatI CATG 8 cut(s) 355, 582, 877, 1009, 1661, 1908, 2220, 2288
FauI CCCGC 1 cut(s) 1713
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 1363
Fnu4HI GCNGC 8 cut(s) 24, 41, 570, 863, 948, 1101, 1757, 2213
FriOI GRGCYC 2 cut(s) 15, 2064
Fsp4HI GCNGC 8 cut(s) 24, 41, 570, 863, 948, 1101, 1757, 2213
FspBI CTAG 8 cut(s) 327, 719, 1410, 1677, 1982, 2177, 2192, 2207
GlaI GCGC 2 cut(s) 64, 2090
GluI GCNGC 8 cut(s) 24, 41, 570, 863, 948, 1101, 1757, 2213
GsuI CTGGAG 2 cut(s) 318, 850
HaeIII GGCC 5 cut(s) 318, 454, 1119, 2138, 2190
HgaI GACGC 1 cut(s) 279
HhaI GCGC 2 cut(s) 65, 2091
Hin1II CATG 8 cut(s) 359, 586, 881, 1013, 1665, 1912, 2224, 2292
Hin6I GCGC 2 cut(s) 63, 2089
HinP1I GCGC 2 cut(s) 63, 2089
HincII GTYRAC 2 cut(s) 1027, 1992
HindII GTYRAC 2 cut(s) 1027, 1992
HindIII AAGCTT 2 cut(s) 2393, 2772
HphI GGTGA 2 cut(s) 118, 1784
Hpy166II GTNNAC 7 cut(s) 109, 464, 958, 1027, 1364, 1618, 1992
Hpy8I GTNNAC 7 cut(s) 109, 464, 958, 1027, 1364, 1618, 1992
Hpy99I CGWCG 1 cut(s) 273
HpyAV CCTTC 8 cut(s) 26, 41, 68, 76, 1319, 1885, 1958, 2391
HpyCH4IV ACGT 4 cut(s) 466, 731, 1452, 1862
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 140, 1717, 2034, 2068, 2135
HpyF3I CTNAG 7 cut(s) 612, 735, 1611, 1937, 2252, 2387, 2757
HpySE526I ACGT 4 cut(s) 466, 731, 1452, 1862
Hsp92II CATG 8 cut(s) 359, 586, 881, 1013, 1665, 1912, 2224, 2292
HspAI GCGC 2 cut(s) 63, 2089
KpnI GGTACC 1 cut(s) 1795
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 8 cut(s) 205, 376, 655, 847, 1305, 1798, 2478, 2533
LmnI GCTCC 4 cut(s) 18, 831, 884, 2546
Lsp1109I GCAGC 7 cut(s) 27, 35, 556, 849, 1112, 1743, 2199
LweI GCATC 8 cut(s) 145, 541, 849, 896, 1238, 2080, 2116, 2639
MaeI CTAG 8 cut(s) 327, 719, 1410, 1677, 1982, 2177, 2192, 2207
MaeII ACGT 4 cut(s) 466, 731, 1452, 1862
MalI GATC 8 cut(s) 207, 378, 657, 849, 1307, 1800, 2480, 2535
MboI GATC 8 cut(s) 205, 376, 655, 847, 1305, 1798, 2478, 2533
MfeI CAATTG 2 cut(s) 135, 1211
MflI RGATCY 2 cut(s) 1798, 2478
MhlI GDGCHC 4 cut(s) 15, 836, 1728, 2064
MlyI GAGTC 3 cut(s) 309, 457, 1102
MmeI TCCRAC 8 cut(s) 180, 472, 862, 971, 1140, 1941, 2313, 2574
Mph1103I ATGCAT 1 cut(s) 1461
MroXI GAANNNNTTC 1 cut(s) 2038
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 2 cut(s) 950, 1756
MspCI CTTAAG 1 cut(s) 659
MspR9I CCNGG 4 cut(s) 242, 588, 1097, 1796
MunI CAATTG 2 cut(s) 135, 1211
Mva1269I GAATGC 2 cut(s) 26, 1974
MvaI CCWGG 4 cut(s) 242, 588, 1097, 1796
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 6 cut(s) 71, 140, 1717, 2034, 2068, 2135
NdeII GATC 8 cut(s) 205, 376, 655, 847, 1305, 1798, 2478, 2533
NlaIII CATG 8 cut(s) 359, 586, 881, 1013, 1665, 1912, 2224, 2292
NlaIV GGNNCC 2 cut(s) 317, 1793
NmeAIII GCCGAG 1 cut(s) 34
NmuCI GTSAC 3 cut(s) 635, 727, 1190
NsiI ATGCAT 1 cut(s) 1461
NspI RCATGY 3 cut(s) 586, 1013, 2224
PaeI GCATGC 1 cut(s) 586
PciI ACATGT 1 cut(s) 2220
PctI GAATGC 2 cut(s) 26, 1974
PdmI GAANNNNTTC 1 cut(s) 2038
PfeI GAWTC 7 cut(s) 93, 722, 772, 1832, 1915, 2614, 2641
PflMI CCANNNNNTGG 1 cut(s) 2369
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 8 cut(s) 25, 42, 571, 864, 949, 1102, 1758, 2214
PleI GAGTC 3 cut(s) 308, 456, 1102
PmaCI CACGTG 1 cut(s) 467
PmlI CACGTG 1 cut(s) 467
PpsI GAGTC 3 cut(s) 308, 456, 1102
Ppu21I YACGTR 2 cut(s) 467, 1453
PscI ACATGT 1 cut(s) 2220
PshAI GACNNNNGTC 1 cut(s) 447
PshBI ATTAAT 2 cut(s) 624, 1923
PsiI TTATAA 1 cut(s) 2402
Psp124BI GAGCTC 1 cut(s) 15
Psp6I CCWGG 4 cut(s) 240, 586, 1095, 1794
PspCI CACGTG 1 cut(s) 467
PspEI GGTNACC 1 cut(s) 2604
PspGI CCWGG 4 cut(s) 240, 586, 1095, 1794
PspN4I GGNNCC 2 cut(s) 317, 1793
PspPI GGNCC 4 cut(s) 316, 751, 2136, 2432
PsuI RGATCY 2 cut(s) 1798, 2478
PvuII CAGCTG 1 cut(s) 1756
RsaI GTAC 4 cut(s) 312, 1617, 1793, 2737
RsaNI GTAC 4 cut(s) 311, 1616, 1792, 2736
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 15
SatI GCNGC 8 cut(s) 24, 41, 570, 863, 948, 1101, 1757, 2213
Sau3AI GATC 8 cut(s) 205, 376, 655, 847, 1305, 1798, 2478, 2533
Sau96I GGNCC 4 cut(s) 316, 751, 2136, 2432
ScaI AGTACT 1 cut(s) 2737
SchI GAGTC 3 cut(s) 309, 457, 1102
ScrFI CCNGG 4 cut(s) 242, 588, 1097, 1796
SduI GDGCHC 4 cut(s) 15, 836, 1728, 2064
SfaNI GCATC 8 cut(s) 145, 541, 849, 896, 1238, 2080, 2116, 2639
SinI GGWCC 2 cut(s) 751, 2432
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 4 cut(s) 659, 697, 2162, 2483
SmoI CTYRAG 4 cut(s) 659, 697, 2162, 2483
SnaBI TACGTA 1 cut(s) 1453
SpeI ACTAGT 1 cut(s) 1676
SphI GCATGC 1 cut(s) 586
SsiI CCGC 6 cut(s) 143, 158, 948, 1627, 1720, 1789
SspI AATATT 1 cut(s) 1391
SspMI CTAG 8 cut(s) 327, 719, 1410, 1677, 1982, 2177, 2192, 2207
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 4 cut(s) 240, 586, 1095, 1794
StyI CCWWGG 1 cut(s) 2191
TaiI ACGT 4 cut(s) 469, 734, 1455, 1865
TaqI TCGA 6 cut(s) 379, 654, 1430, 1510, 2077, 2727
TatI WGTACW 1 cut(s) 2735
TauI GCSGC 1 cut(s) 950
TfiI GAWTC 7 cut(s) 93, 722, 772, 1832, 1915, 2614, 2641
TscAI CASTG 7 cut(s) 262, 508, 640, 857, 1402, 2013, 2475
TseFI GTSAC 3 cut(s) 635, 727, 1190
TseI GCWGC 7 cut(s) 23, 40, 569, 862, 1100, 1756, 2212
Tsp45I GTSAC 3 cut(s) 635, 727, 1190
TspDTI ATGAA 8 cut(s) 58, 365, 372, 402, 866, 1299, 1925, 2513
TspRI CASTG 7 cut(s) 262, 508, 640, 857, 1402, 2013, 2475
Van91I CCANNNNNTGG 1 cut(s) 2369
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 751, 2432
VspI ATTAAT 2 cut(s) 624, 1923
XapI RAATTY 6 cut(s) 524, 1045, 1286, 1441, 1500, 2279
XceI RCATGY 3 cut(s) 586, 1013, 2224
XmaJI CCTAGG 1 cut(s) 2191
XmiI GTMKAC 1 cut(s) 1363
XmnI GAANNNNTTC 1 cut(s) 2038
XspI CTAG 8 cut(s) 327, 719, 1410, 1677, 1982, 2177, 2192, 2207
ZrmI AGTACT 1 cut(s) 2737
Zsp2I ATGCAT 1 cut(s) 1461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.