MD11G1040600.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
3473373 .. 3476644
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1040600.v1.1.491

Sequence Viewer

Length: 2931 bp
ATGGCACTGGGAGAGGTTTTTCTTGCGGCGTTTCTACAGTTGCTGCTCGACAGGTTGACCCCTCGCGAGATTCTTGAGTACGTTGGAAACGTCCGGGGGGTCGGACAGAAGCTGGAGAGGTGGAGGACCACGTTGTCTACAATCGGAGCGGTGCTGAGCGACGCCGAGGAAAGGCAACTGACTGAAGGTGGTGTGAAACTGTGGCTGGATGATCTCAGAGACCTGGCCTATGATATCGAAGACATGTTGGACAAATTTGCCGTTAAAATGTTGAAGCGCATGATAGAGGGACGTGATCAAGCCAGCACAAGCAGGAAGGTACGGAGATCATTTTATAAAGTTAAATTGAGTTTTGATATGAACTCCGAAATGAAGAAGATTACGAAGCGGTTGCAAGACATATCTGAACGGAAAGATAAGTTTGGCTTGAAAGATACTGGGACGTCTGCTAAGGAATCTCGAAGTTTACCAAGTTCAGGTGTGTTAGATGAAAAGCTTGTTGTTGGAAGAGATGGTGACAAAGGGAAGATTATTGAATTGTTGAAAAAGTCTACCGATTTTGGTGTAGTTGCTATAGTTGGCATGCCCGGAGTTGGGAAGACGACACTTGCTCAACTTGTATTCAACCACAAAAATGATGCCATGAAGGAGTTTGAGCTAAAGGTGTGGGTATCTGTGTCTGATGACTTCAATGTTGTGCGAGTGACAAAGGAAATTCTTGAATCAATCACATCCCGACCCGTCCAAGTGGAGGGGTTTAGTAAAATGCAGCATGATTTGAGTGAGCAATTAAGAGGAAAAAAGTTTTTAATCGTTTTAGATGATATCTGGAACAAAGATGACTCTGATCTATACGATCTCTGGACAAGACTTCAATCCCCTTTTGGCATCGGAGCAGGAGGAAGTAAGATTATTGTGACAACCCGTGATGTGAATGTTGCAAAGATTATGGGAGCCACTGGAGTTCATAATTTGGAGTGTATGGCAGATGGTGATTGTTTGGAAATATTTGAGCGACATGCGTTCAGGGGAATTAATAATGGAAAGCCGGTAAATTATGGTTTAATTAAGACAAGAATTGTTGAAAAATGTCGTGGCTTACCATTAGCTGCAAGGACTCTCGGTGGTCTTTTACGTTGCAAAGAAAAAGATGAGTGGGGAGAAATATTGAACAACAAGTTATGGAATCTAGCAGACAAGAGTGGCATTCTCCCCGTACTAAAGTTGAGCTATCACTATCTTCCATCAAATTTGAAGAGGTGTTTTGCATATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGAAGCAGCTCATTCTTTTGTGGATGGCAGAGGGTTTGATTCAACAAAAACATGATGACAATAAACAAATGGAGGACTTGGGCCGCGACTACTTTCGAGAGCTATTAGCAAGGTCATTGTTTCAAGAATCAAGCAAAAACAATTCACGATATGTAATGCATGACCTCGTTAATGATTTAGCACAATGGGCAGCAGGTGAAATATGTTTTAGGTTGGAAGATAAGCGAGGTGATAACTTGCCAAGCACTTGCTTTCGAAGGGCTCGCCATTCGTCTTTCATTGCTGGTCAATTTGATGGATTTACGAGATTTGAGGCCTTTCCAAAAGCTGAACATTTGCGAACATTCCTGCCACTTTCACTTTCAAATTCCAGGGTATGGCACACATATTTGCCTCGTAAGGTTACTTTTGAGCTATTACCACAGTTGCAATACTTACGAGTGCTCTCGTTCAATGGCTACAAAATAACTGAGCTGCCAGAGTCAATCGGTGATTTGAGGTTGTTACAGTATCTTGACCTTTCCTATACATATATAGCCAGTTTGCCTAAATCAACAAGCACTCTTTACCACTTGCAAACATTGATATTGGAAGGTTGTTCTCAATTGAAGTCATTGCCCGCGAACATGAGTAATCTAATTAATTTGCGCCATCTCAACAACTCATATGCATCTTCGTTGAAAGGAATGCCTTCGCAACTAGGTCGATTGACAAATCTACAATCACTGCCTCTTTTTGTGGTGAGCGAAGGAAGTGATCATTCAGGGATAAGAGAGATAGGGCCCCTATTGCATCTCCGAGGGACATTGTGCCTCTCAGGATTGGAGAATGTGACTAATGTCGAGGATGCCAGGAGGGCCAACTTGAAATGCAAGGAGAGGCTTGATTCACTGGTCCTAAAATGGTATTATTCAAGCGACACGAGAGAAACAGAATCCGCTGTGCTTGACATGTTACAGCCTCATACAAAGCTCAAGGAGCTCACCATCACGGGTTATGCCAGAGAGGAATTTTCATCATGGGTTGGAGGTCCATTGTTCTCTAATATGGTGCTTGTGCGCTTAGAGGAATGTAACAATTGTTTATCGTTGCCACCTCTCGGACAATTGCCTCGTCTCAAAGAGCTTTATATTAGAGGAATGAATGCAGTCGAAAGTGTTGGTGCTGAGTTTTATGGTGAGTGTGTCATGCCTTTTCCGCTGTTAGAGATTCTCAAGTTTGTGAATATGCGGCATTGGAAGGTGTGGCTTCCTTTCCAACTGGATCACGGAAGTGGTGTTTTCCCTTGCCTAAAAAGGCTTTCAATCAAGGAATGTTCTAAGTTGGAAGGTAAACTGCCAGAGAAGCTCGATTTGTTAGCCAAACTTGAAATTGTTAAATGTGAGGAATTGACGGTTTCGATTGCCAACTACAAACAGCTTCGTCAGCTAAACATTGACGGTTGTAAAGTGTTGGAACATACGGCTGCTAAGGTTGAGTTTGAGTTATTAGAGTCCTTGTGCATTTCAAACATTTCGGAGGTGATGTCTCGGCCAACAGGGGAATTGTTCAGAAAGGGACTAAGCAAGGTTAGAGATTTGAAGATCAATGGATTGGTTGACTTCTCTTGGCCGTTTGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

977

Amino Acids

110.88

Weight (kDa)

8.53

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 102 1.5e-21 Rx N-terminal domain
NB-ARC PF00931 172 - 343 4e-38 NB-ARC domain
WHD_DRP PF23559 427 - 496 6.5e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 574 - 872 5.7e-24 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 692 - 816 2.4e-35 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AarI CACCTGC 1 cut(s) 1490
AasI GACNNNNNNGTC 1 cut(s) 133
AatII GACGTC 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 1490
AccB7I CCANNNNNTGG 1 cut(s) 1683
AccBSI CCGCTC 1 cut(s) 149
AccI GTMKAC 2 cut(s) 137, 551
AccII CGCG 3 cut(s) 66, 1392, 1928
AciI CCGC 8 cut(s) 26, 149, 388, 1390, 1926, 2244, 2504, 2536
AclWI GGATC 1 cut(s) 2577
AcoI YGGCCR 2 cut(s) 2837, 2915
AcsI RAATTY 6 cut(s) 254, 714, 1249, 1298, 1672, 2315
AcuI CTGAAG 1 cut(s) 204
AcyI GRCGYC 2 cut(s) 162, 443
AfaI GTAC 3 cut(s) 80, 321, 1218
AfiI CCNNNNNNNGG 7 cut(s) 171, 476, 593, 594, 751, 1683, 2405
AflIII ACRYGT 2 cut(s) 243, 2256
AjiI CACGTC 1 cut(s) 293
AjnI CCWGG 3 cut(s) 222, 1676, 2156
AleI CACNNNNGTG 1 cut(s) 2577
Alw21I GWGCWC 2 cut(s) 1752, 2289
Alw26I GTCTC 3 cut(s) 213, 2426, 2838
AlwI GGATC 1 cut(s) 2577
AlwNI CAGNNNCTG 2 cut(s) 43, 112
AoxI GGCC 7 cut(s) 225, 1387, 1620, 2087, 2163, 2837, 2915
ApaI GGGCCC 1 cut(s) 2091
ApeKI GCWGC 7 cut(s) 43, 769, 1109, 1312, 1496, 1780, 2771
ApoI RAATTY 6 cut(s) 254, 714, 1249, 1298, 1672, 2315
ArsI GACNNNNNNTTYG 2 cut(s) 1283, 1315
AseI ATTAAT 2 cut(s) 1035, 1947
Asp700I GAANNNNTTC 1 cut(s) 1996
AspLEI GCGC 3 cut(s) 279, 1956, 2367
AspS9I GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
AsuC2I CCSGG 2 cut(s) 95, 588
AsuHPI GGTGA 9 cut(s) 527, 1004, 1514, 1547, 1808, 2059, 2281, 2495, 2839
AsuII TTCGAA 1 cut(s) 1561
AvaII GGWCC 3 cut(s) 126, 2200, 2336
BaeGI GKGCMC 1 cut(s) 2091
BanII GRGCYC 3 cut(s) 1570, 2091, 2289
BauI CACGAG 1 cut(s) 2227
BbsI GAAGAC 2 cut(s) 246, 605
Bbv12I GWGCWC 2 cut(s) 1752, 2289
BbvI GCAGC 7 cut(s) 30, 781, 1096, 1324, 1508, 1767, 2758
BccI CCATC 7 cut(s) 506, 983, 1252, 1324, 1595, 1965, 2300
BceAI ACGGC 3 cut(s) 245, 2784, 2902
BcgI CGANNNNNNTGC 4 cut(s) 373, 407, 1991, 2025
BciT130I CCWGG 3 cut(s) 224, 1678, 2158
BclI TGATCA 2 cut(s) 295, 2062
BcnI CCSGG 2 cut(s) 95, 588
BcoDI GTCTC 3 cut(s) 213, 2426, 2838
BfaI CTAG 2 cut(s) 1190, 2006
BfmI CTRYAG 2 cut(s) 35, 573
BfuAI ACCTGC 1 cut(s) 1490
BglI GCCNNNNNGGC 1 cut(s) 2162
BlpI GCTNAGC 1 cut(s) 155
Bme1390I CCNGG 5 cut(s) 95, 224, 588, 1678, 2158
Bme18I GGWCC 3 cut(s) 126, 2200, 2336
BmgBI CACGTC 1 cut(s) 293
BmgT120I GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
BmiI GGNNCC 3 cut(s) 955, 2089, 2090
BmrFI CCNGG 5 cut(s) 95, 224, 588, 1678, 2158
BmrI ACTGGG 2 cut(s) 17, 447
BmsI GCATC 5 cut(s) 628, 897, 1985, 2107, 2143
BmuI ACTGGG 2 cut(s) 17, 447
BoxI GACNNNNGTC 1 cut(s) 2144
BpiI GAAGAC 2 cut(s) 246, 605
BpmI CTGGAG 2 cut(s) 134, 981
Bpu10I CCTNAGC 2 cut(s) 450, 2775
Bpu1102I GCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 1561
BpuEI CTTGAG 3 cut(s) 95, 2264, 2504
BpuMI CCSGG 2 cut(s) 95, 588
BsaHI GRCGYC 2 cut(s) 162, 443
BsaI GGTCTC 1 cut(s) 213
BsaJI CCNNGG 4 cut(s) 94, 165, 1677, 2104
BsaXI ACNNNNNCTCC 2 cut(s) 2816, 2846
Bsc4I CCNNNNNNNGG 7 cut(s) 171, 476, 593, 594, 751, 1683, 2405
Bse118I RCCGGY 1 cut(s) 1048
Bse1I ACTGG 6 cut(s) 12, 442, 964, 1845, 2202, 2571
Bse3DI GCAATG 2 cut(s) 1584, 1919
BseBI CCWGG 3 cut(s) 224, 1678, 2158
BseDI CCNNGG 4 cut(s) 94, 165, 1677, 2104
BseGI GGATG 4 cut(s) 214, 731, 1335, 2158
BseLI CCNNNNNNNGG 7 cut(s) 171, 476, 593, 594, 751, 1683, 2405
BseMI GCAATG 2 cut(s) 1584, 1919
BseMII CTCAG 5 cut(s) 146, 229, 1767, 2136, 2463
BseNI ACTGG 6 cut(s) 12, 442, 964, 1845, 2202, 2571
BseSI GKGCMC 1 cut(s) 2091
BseXI GCAGC 7 cut(s) 30, 781, 1096, 1324, 1508, 1767, 2758
Bsh1236I CGCG 3 cut(s) 66, 1392, 1928
BshFI GGCC 7 cut(s) 227, 1389, 1622, 2089, 2165, 2839, 2917
BsiHKAI GWGCWC 2 cut(s) 1752, 2289
BsiSI CCGG 3 cut(s) 94, 588, 1049
BslFI GGGAC 4 cut(s) 303, 454, 2122, 2877
BslI CCNNNNNNNGG 7 cut(s) 171, 476, 593, 594, 751, 1683, 2405
BsmAI GTCTC 3 cut(s) 213, 2426, 2838
BsmBI CGTCTC 1 cut(s) 2426
BsmFI GGGAC 4 cut(s) 303, 454, 2122, 2877
BsmI GAATGC 3 cut(s) 1206, 1998, 2455
BsnI GGCC 7 cut(s) 227, 1389, 1622, 2089, 2165, 2839, 2917
Bso31I GGTCTC 1 cut(s) 213
Bsp119I TTCGAA 1 cut(s) 1561
Bsp120I GGGCCC 1 cut(s) 2087
Bsp1286I GDGCHC 4 cut(s) 1570, 1752, 2091, 2289
Bsp143I GATC 8 cut(s) 211, 295, 326, 847, 856, 2062, 2569, 2889
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp68I TCGCGA 1 cut(s) 66
BspACI CCGC 8 cut(s) 26, 149, 388, 1390, 1926, 2244, 2504, 2536
BspANI GGCC 7 cut(s) 227, 1389, 1622, 2089, 2165, 2839, 2917
BspCNI CTCAG 5 cut(s) 147, 228, 1768, 2135, 2464
BspFNI CGCG 3 cut(s) 66, 1392, 1928
BspLI GGNNCC 3 cut(s) 955, 2089, 2090
BspMI ACCTGC 1 cut(s) 1490
BspPI GGATC 1 cut(s) 2577
BspT104I TTCGAA 1 cut(s) 1561
BspTNI GGTCTC 1 cut(s) 213
BsrBI CCGCTC 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 1584, 1919
BsrFI RCCGGY 1 cut(s) 1048
BsrI ACTGG 6 cut(s) 12, 442, 964, 1845, 2202, 2571
BssAI RCCGGY 1 cut(s) 1048
BssECI CCNNGG 4 cut(s) 94, 165, 1677, 2104
BssMI GATC 8 cut(s) 211, 295, 326, 847, 856, 2062, 2569, 2889
BssNI GRCGYC 2 cut(s) 162, 443
BssSI CACGAG 1 cut(s) 2227
Bst2BI CACGAG 1 cut(s) 2227
Bst2UI CCWGG 3 cut(s) 224, 1678, 2158
Bst4CI ACNGT 6 cut(s) 39, 201, 1731, 1815, 2701, 2747
Bst6I CTCTTC 2 cut(s) 502, 1250
BstACI GRCGYC 2 cut(s) 162, 443
BstBI TTCGAA 1 cut(s) 1561
BstC8I GCNNGC 4 cut(s) 304, 584, 1570, 1926
BstF5I GGATG 4 cut(s) 214, 731, 1335, 2158
BstFNI CGCG 3 cut(s) 66, 1392, 1928
BstHHI GCGC 3 cut(s) 279, 1956, 2367
BstKTI GATC 8 cut(s) 214, 298, 329, 850, 859, 2065, 2572, 2892
BstMAI GTCTC 3 cut(s) 213, 2426, 2838
BstMBI GATC 8 cut(s) 211, 295, 326, 847, 856, 2062, 2569, 2889
BstMWI GCNNNNNNNGC 7 cut(s) 1493, 2095, 2162, 2284, 2503, 2650, 2731
BstNI CCWGG 3 cut(s) 224, 1678, 2158
BstNSI RCATGY 4 cut(s) 247, 586, 1022, 2260
BstPAI GACNNNNGTC 1 cut(s) 2144
BstSCI CCNGG 5 cut(s) 93, 222, 586, 1676, 2156
BstSFI CTRYAG 2 cut(s) 35, 573
BstSLI GKGCMC 1 cut(s) 2091
BstUI CGCG 3 cut(s) 66, 1392, 1928
BstV1I GCAGC 7 cut(s) 30, 781, 1096, 1324, 1508, 1767, 2758
BstV2I GAAGAC 2 cut(s) 246, 605
BsuRI GGCC 7 cut(s) 227, 1389, 1622, 2089, 2165, 2839, 2917
BtrI CACGTC 1 cut(s) 293
BtsCI GGATG 4 cut(s) 214, 731, 1335, 2158
BtsI GCAGTG 1 cut(s) 2030
BtsIMutI CAGTG 4 cut(s) 5, 957, 2030, 2195
BtuMI TCGCGA 1 cut(s) 66
BveI ACCTGC 1 cut(s) 1490
Cac8I GCNNGC 4 cut(s) 304, 584, 1570, 1926
CaiI CAGNNNCTG 2 cut(s) 43, 112
CfoI GCGC 3 cut(s) 279, 1956, 2367
Cfr10I RCCGGY 1 cut(s) 1048
Cfr13I GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
CseI GACGC 1 cut(s) 170
Csp6I GTAC 3 cut(s) 79, 320, 1217
CviQI GTAC 3 cut(s) 79, 320, 1217
DpnI GATC 8 cut(s) 213, 297, 328, 849, 858, 2064, 2571, 2891
DpnII GATC 8 cut(s) 211, 295, 326, 847, 856, 2062, 2569, 2889
DrdI GACNNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 2 cut(s) 2837, 2915
Eam1104I CTCTTC 2 cut(s) 502, 1250
EarI CTCTTC 2 cut(s) 502, 1250
Ecl136II GAGCTC 1 cut(s) 2287
Eco147I AGGCCT 1 cut(s) 1622
Eco24I GRGCYC 3 cut(s) 1570, 2091, 2289
Eco31I GGTCTC 1 cut(s) 213
Eco32I GATATC 2 cut(s) 235, 826
Eco47I GGWCC 3 cut(s) 126, 2200, 2336
Eco53kI GAGCTC 1 cut(s) 2287
Eco57I CTGAAG 1 cut(s) 204
EcoICRI GAGCTC 1 cut(s) 2287
EcoO109I RGGNCCY 2 cut(s) 2087, 2088
EcoRII CCWGG 3 cut(s) 222, 1676, 2156
EcoRV GATATC 2 cut(s) 235, 826
EcoT22I ATGCAT 2 cut(s) 1467, 1978
EcoT38I GRGCYC 3 cut(s) 1570, 2091, 2289
Esp3I CGTCTC 1 cut(s) 2426
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 4 cut(s) 303, 454, 2122, 2877
FauI CCCGC 1 cut(s) 1933
FauNDI CATATG 1 cut(s) 1972
FbaI TGATCA 2 cut(s) 295, 2062
FblI GTMKAC 2 cut(s) 137, 551
FokI GGATG 4 cut(s) 221, 718, 1342, 2165
FriOI GRGCYC 3 cut(s) 1570, 2091, 2289
FspBI CTAG 2 cut(s) 1190, 2006
GlaI GCGC 3 cut(s) 278, 1955, 2366
GsuI CTGGAG 2 cut(s) 134, 981
HaeIII GGCC 7 cut(s) 227, 1389, 1622, 2089, 2165, 2839, 2917
HapII CCGG 3 cut(s) 94, 588, 1049
HgaI GACGC 1 cut(s) 170
HhaI GCGC 3 cut(s) 279, 1956, 2367
Hin1I GRCGYC 2 cut(s) 162, 443
Hin6I GCGC 3 cut(s) 277, 1954, 2365
HinP1I GCGC 3 cut(s) 277, 1954, 2365
HincII GTYRAC 2 cut(s) 57, 2905
HindII GTYRAC 2 cut(s) 57, 2905
HindIII AAGCTT 1 cut(s) 494
HpaII CCGG 3 cut(s) 94, 588, 1049
HphI GGTGA 9 cut(s) 527, 1004, 1514, 1547, 1808, 2059, 2281, 2495, 2839
Hpy166II GTNNAC 6 cut(s) 57, 138, 467, 552, 2639, 2905
Hpy8I GTNNAC 6 cut(s) 57, 138, 467, 552, 2639, 2905
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 9 cut(s) 179, 310, 640, 1557, 1892, 2007, 2048, 2539, 2627
HpyCH4III ACNGT 6 cut(s) 39, 201, 1731, 1815, 2701, 2747
HpyCH4IV ACGT 6 cut(s) 81, 90, 131, 292, 443, 1135
HpyF10VI GCNNNNNNNGC 7 cut(s) 1493, 2095, 2162, 2284, 2503, 2650, 2731
HpySE526I ACGT 6 cut(s) 81, 90, 131, 292, 443, 1135
Hsp92I GRCGYC 2 cut(s) 162, 443
HspAI GCGC 3 cut(s) 277, 1954, 2365
Ksp22I TGATCA 2 cut(s) 295, 2062
Kzo9I GATC 8 cut(s) 211, 295, 326, 847, 856, 2062, 2569, 2889
LmnI GCTCC 4 cut(s) 146, 893, 953, 2284
Lsp1109I GCAGC 7 cut(s) 30, 781, 1096, 1324, 1508, 1767, 2758
LweI GCATC 5 cut(s) 628, 897, 1985, 2107, 2143
MaeI CTAG 2 cut(s) 1190, 2006
MaeII ACGT 6 cut(s) 81, 90, 131, 292, 443, 1135
MaeIII GTNAC 8 cut(s) 515, 703, 916, 1708, 1809, 2137, 2259, 2378
MalI GATC 8 cut(s) 213, 297, 328, 849, 858, 2064, 2571, 2891
MbiI CCGCTC 1 cut(s) 149
MboI GATC 8 cut(s) 211, 295, 326, 847, 856, 2062, 2569, 2889
MfeI CAATTG 3 cut(s) 1910, 2383, 2411
MhlI GDGCHC 4 cut(s) 1570, 1752, 2091, 2289
MlyI GAGTC 4 cut(s) 836, 1111, 1796, 2807
MmeI TCCRAC 9 cut(s) 64, 82, 228, 484, 1500, 2311, 2587, 2610, 2739
Mph1103I ATGCAT 2 cut(s) 1467, 1978
MroXI GAANNNNTTC 1 cut(s) 1996
MslI CAYNNNNRTG 2 cut(s) 633, 2577
MspA1I CMGCKG 2 cut(s) 2246, 2506
MspI CCGG 3 cut(s) 94, 588, 1049
MspR9I CCNGG 5 cut(s) 95, 224, 588, 1678, 2158
MunI CAATTG 3 cut(s) 1910, 2383, 2411
Mva1269I GAATGC 3 cut(s) 1206, 1998, 2455
MvaI CCWGG 3 cut(s) 224, 1678, 2158
MvnI CGCG 3 cut(s) 66, 1392, 1928
MwoI GCNNNNNNNGC 7 cut(s) 1493, 2095, 2162, 2284, 2503, 2650, 2731
NciI CCSGG 2 cut(s) 95, 588
NdeI CATATG 1 cut(s) 1972
NdeII GATC 8 cut(s) 211, 295, 326, 847, 856, 2062, 2569, 2889
NlaIV GGNNCC 3 cut(s) 955, 2089, 2090
NmeAIII GCCGAG 2 cut(s) 190, 2815
NmuCI GTSAC 4 cut(s) 515, 703, 916, 2137
NruI TCGCGA 1 cut(s) 66
NsiI ATGCAT 2 cut(s) 1467, 1978
NspI RCATGY 4 cut(s) 247, 586, 1022, 2260
NspV TTCGAA 1 cut(s) 1561
OliI CACNNNNGTG 1 cut(s) 2577
PacI TTAATTAA 1 cut(s) 1068
PaeI GCATGC 1 cut(s) 586
PaqCI CACCTGC 1 cut(s) 1490
PceI AGGCCT 1 cut(s) 1622
PciI ACATGT 2 cut(s) 243, 2256
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 3 cut(s) 1206, 1998, 2455
PdmI GAANNNNTTC 1 cut(s) 1996
PfeI GAWTC 9 cut(s) 70, 455, 722, 1186, 1345, 1433, 2192, 2240, 2515
PflMI CCANNNNNTGG 1 cut(s) 1683
PleI GAGTC 4 cut(s) 836, 1111, 1795, 2806
PpsI GAGTC 4 cut(s) 836, 1111, 1795, 2806
PscI ACATGT 2 cut(s) 243, 2256
PshAI GACNNNNGTC 1 cut(s) 2144
PshBI ATTAAT 2 cut(s) 1035, 1947
PsiI TTATAA 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 2289
Psp6I CCWGG 3 cut(s) 222, 1676, 2156
PspGI CCWGG 3 cut(s) 222, 1676, 2156
PspN4I GGNNCC 3 cut(s) 955, 2089, 2090
PspOMI GGGCCC 1 cut(s) 2087
PspPI GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
PstNI CAGNNNCTG 2 cut(s) 43, 112
RruI TCGCGA 1 cut(s) 66
RsaI GTAC 3 cut(s) 80, 321, 1218
RsaNI GTAC 3 cut(s) 79, 320, 1217
RseI CAYNNNNRTG 2 cut(s) 633, 2577
SacI GAGCTC 1 cut(s) 2289
Sau3AI GATC 8 cut(s) 211, 295, 326, 847, 856, 2062, 2569, 2889
Sau96I GGNCC 7 cut(s) 126, 1387, 2087, 2088, 2163, 2200, 2336
SchI GAGTC 4 cut(s) 836, 1111, 1796, 2807
ScrFI CCNGG 5 cut(s) 95, 224, 588, 1678, 2158
SduI GDGCHC 4 cut(s) 1570, 1752, 2091, 2289
SfaNI GCATC 5 cut(s) 628, 897, 1985, 2107, 2143
SfcI CTRYAG 2 cut(s) 35, 573
SfuI TTCGAA 1 cut(s) 1561
SinI GGWCC 3 cut(s) 126, 2200, 2336
SmiMI CAYNNNNRTG 2 cut(s) 633, 2577
SmlI CTYRAG 3 cut(s) 74, 2279, 2519
SmoI CTYRAG 3 cut(s) 74, 2279, 2519
SphI GCATGC 1 cut(s) 586
SseBI AGGCCT 1 cut(s) 1622
SsiI CCGC 8 cut(s) 26, 149, 388, 1390, 1926, 2244, 2504, 2536
SspI AATATT 2 cut(s) 1008, 1167
SspMI CTAG 2 cut(s) 1190, 2006
SstI GAGCTC 1 cut(s) 2289
StuI AGGCCT 1 cut(s) 1622
StyD4I CCNGG 5 cut(s) 93, 222, 586, 1676, 2156
TaaI ACNGT 6 cut(s) 39, 201, 1731, 1815, 2701, 2747
TaiI ACGT 6 cut(s) 84, 93, 134, 295, 446, 1138
TauI GCSGC 3 cut(s) 29, 1392, 2539
TfiI GAWTC 9 cut(s) 70, 455, 722, 1186, 1345, 1433, 2192, 2240, 2515
TscAI CASTG 4 cut(s) 12, 964, 2037, 2202
TseFI GTSAC 4 cut(s) 515, 703, 916, 2137
TseI GCWGC 7 cut(s) 43, 769, 1109, 1312, 1496, 1780, 2771
Tsp45I GTSAC 4 cut(s) 515, 703, 916, 2137
TspDTI ATGAA 9 cut(s) 374, 386, 504, 659, 956, 1311, 1573, 2310, 2462
TspGWI ACGGA 3 cut(s) 337, 424, 2589
TspRI CASTG 4 cut(s) 12, 964, 2037, 2202
Van91I CCANNNNNTGG 1 cut(s) 1683
VpaK11BI GGWCC 3 cut(s) 126, 2200, 2336
VspI ATTAAT 2 cut(s) 1035, 1947
XapI RAATTY 6 cut(s) 254, 714, 1249, 1298, 1672, 2315
XceI RCATGY 4 cut(s) 247, 586, 1022, 2260
XmiI GTMKAC 2 cut(s) 137, 551
XmnI GAANNNNTTC 1 cut(s) 1996
XspI CTAG 2 cut(s) 1190, 2006
ZraI GACGTC 1 cut(s) 444
Zsp2I ATGCAT 2 cut(s) 1467, 1978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.