RchiOBHm_Chr1g0315001

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Forward (+)
2280549 .. 2284045
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54555

Sequence Viewer

Length: 3393 bp
ATGGTGTTGGGAGAGGTCTTTCTCGGGGCATTCCTTCAAGTGCTGCTTGACAGGTTGAGCCCTAGGGCGATCATCAACTTTGCAAGCCTACATGGTGCGAGTGTGGACAAGAAGCTCAAGAAATGGAGTTCAACGCTATCTACAATCGGAGCTGTACTTTATGATGCGGAGGAGAAGCAACTTACGAGCGAGGCTGTCAATCTGTGGCTGGATGATCTCAAAGACTTGGCTTATGATATTGAAGACATCTTAGACAAGTTTTCTATCGAGTTATTACGACGCAGAGTCAAGGAGCAACATGGAGCTGCTACCATAAGTAAGGTACGGAGCCTAATTCCCCAAGCTAAATTCAACTTCCATATGAACTCCAAAATCAAGGAGATCACCGATAGACTCCGAGACATATCTGAACGAAAAGAATCGCTTCGTTTGAACGATATTGGGATGTCTAGTAATCATTATAGGGCATGGAAAATGCCACCGAGTTCGTGTCGACCGGATGGACCTGTGATCGGAAGAGACTGGGATCAAAGCCAGGTTGTTGGATTTTTGTCCGTAGCCGAGCCTAGTGCCATCAATTTTCATGTTGTGGCCATTCTTGGCATGGCAGGAGTTGGAAAGACAACACTTGCAGGACATGTATTCAACAACGATGATGATGCCATGCAACAGTTTGACCTTAAGGTGTGGGTGTATGTGTCTGATGAATTCAATATAGTGAGAGTGACAAAGGCAATTCTCGAATCAATTACATCGGAGCACTGTAAGTTGAAGGAGTTCAGTAAAGTCCAGGATAATCTCAGTAAGGAGTTGACGGGCAAAAAGTTTCTCGTTGTTTTAGATGATGTTTGGGATACATGTGACTACGATATGTGGACCAAATTGCAATCTCCCTTCCGTGTCGGAGCACTAGGAAGTAAGATACTGGTGACAACGCGCCATGAATCAGTTGCAAGAATGATGGGAGCCATACAAGTTCATAATTTGAAGTGTATATCAAGTGATGATTGTTGGCAGGTATTTGAACAACATTCATCCTTGAACATTAACAACAGCAGACCACATAATTTTGAGTCTCTTCGGGACAAGATTACTGCAAAATGCAATGGATTGCCGTTGGCTGCAAGGACGCTTGGTGGACTTTTACGGTGCAAAGAAATTGGTGAATGGGAAGAAATATTGGACAACAAATTATGGAGTCTATCAGATAAGAGTGGTATTCTCCCAGTATTGAAACTGAGCTATCACTCTCTCCCTTCGAACCTAAAAAGATGCTTTGCTTATTGCTCAATACTTCCAAATGACTATGAATTCAGTGAGAAGCAGTTGATCCTTCTATGGATGGCAGATGGTTTGATACTGCATCCAGAACAAAGTAGACAAATGGAAGATTTGGGTGGCGAGTATTTTCAGGAGCTATTATCTAGGTCATTATTTCAAAAATCAAACAAGGACAGTTCACAATATGTTATGCATGACCTCATTAGTGAATTGGCACGGTGGGCTGCAGGAGAAGTTTGTTTCAGACTGGAGGACAATGTGGATGATAACATGCAACATCTTAGGTGTTCTCCAAAAGCTCGACATTCTTCTTACATTTCTGGTGAGTTTGATGGGATTACAAAGTTTGTAGCCTTTTCTGAAGCCAAACGTTTGCGGACGTTCCTGCCATTTACACTTTCAGAAAATCCACAGAATTACTTGACCTGTAAGGTTACTCTTGATTTAGTGCCGAAATTGCAATACTTGAGGGTCCTGTCTTTCAATAGCTACCAGATAACCGAGCTACCAGATTCCATAGGTAAACTGAAGTATCTACGGTACCTTGATGTTTCTCACACGCCAATAAGGAGCTTACCTGAATCAACAAGCACACTTTATAACTTACAGGTATTGATATTAGAAAGTTGTTCTCTTTTGGAGGCACTACCCACAAATATGAGGAATCTCATTAATTTGCGTCATCTCAGCAATTCAAGTGTACATTCGTTGAAGGGAATGCCTATGCAACTAGGTCGGTTAACTAATCTGCAAACACTTGCTGATTTTGTTGTGGGTAAGGGTAGTGAGTCGGGGATAAGAGAGATAGGTCCTCTACTGCATCTCCGGGGGACATTACACCTCTCAAGACTGGAGAATGTGACTGATGTTGAAGATGCAAGGAGGGCTGACTTAATTAGCAAAAAAGAGCTTGATGTCTTGCTATTACAGTGGCATGAGTTGAACGGCACGGGAGAAAGGGAAGCAGATGTTCTTGACATGTTACAACCTCATAGAAAGCTAAAAGAACTCACCATACGGCGATACACTGGTGAAAAAATTGCTGCTTGGGTGGGAAGTCCTTCATTCACTAGCATGGTTCTTGTAAACTTGGAAGATTGTAGTAAATGCAGATTCTTGCCACCACTTGGACAGTTACCTTCTCTCAAGGAGCTTTTTATAAAAGGAATGTCTGGAGTGGAAAGCGTGGGTTCTGAGTTCTATGGAGAGGGTAGCTTGCCGTTTCCACTATTAGAGACCCTTTCATTTCAGAATATGCAAGGATGGAAGGAGTGGATTCCTCTTGAAAGACATCAGGGAACTAAAGATTTTCCTTGCCTGAAAAAGCTTTCCATTTATGCATGCCCCAAACTGGAGGGTGTGGTGCCAGAGAACCTTTATTCATTAGAAGAGCTTGTGGTTCGTGAATGTGACCAACTGGTGGTTTCAATTGCCAACTATAAAGAACTTTCCGTATTATGCATCCGCTATTGCAAAGGGGTTGTATACAGAAGTGTGGTTGACTTTCAGTTATTATATTTGGAGATAACTGGCTGTGAGGAGCTAACATGTTCATTGCAGAATGAGGATGGATTTCTGCAAAACCTTATGTCACTTCGTCATTTGAGTATTGAAGGTAACTCACATCTTGTTCAATTGCAACACCTCAGATTACTACAAGAGCTTCACATTGATAAGTGGCCAAGCCTAGTTTCTTTTCCATTGGCTAGTTTGCCACCTTCTCTTAAAGATATAAAGATTCGAGGTTGCGATTCTCTTACTTATTTTGCAAGATATCAGATACCGCCAAGTCTAAGGAGAATAGAGATAAATAGATGCCAAAATTTGAAATCATTGGTGGAGGATGAGGAGGCGATAGGGGGCTCTTCATCTTCTTCTCCTTGTTTGATTCAGGATGAGGAGTCCTGTCTCGAGTATTTGAGCATATGGAACTGTCCATCTTTGATATCTCTATCATCCAGAGTCAAGCTACCCAGGGCGCTTAAACACCTTCAGATACATACTTGCAGACGACTGGAGTCAATAGCTGATGCATTACATGATAACATTTCTCTCGAGTACATTCACATCTGGAGGGTTCCCAAGAAGTCCTTCCAACTTGAGAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1130

Amino Acids

128.51

Weight (kDa)

6.38

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 100 8.7e-22 Rx N-terminal domain
NB-ARC PF00931 193 - 344 3.2e-37 NB-ARC domain
WHD_DRP PF23559 431 - 499 4.8e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 577 - 847 2.8e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 693 - 817 2.9e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1881, 2441
Acc36I ACCTGC 1 cut(s) 1006
Acc65I GGTACC 1 cut(s) 1821
AccB1I GGYRCC 2 cut(s) 1821, 2644
AccB7I CCANNNNNTGG 2 cut(s) 2408, 2701
AccI GTMKAC 3 cut(s) 493, 1378, 2766
AccII CGCG 1 cut(s) 937
AciI CCGC 4 cut(s) 167, 1657, 2746, 3065
AclI AACGTT 1 cut(s) 1651
AclWI GGATC 2 cut(s) 534, 1324
AcoI YGGCCR 2 cut(s) 591, 2960
AcsI RAATTY 4 cut(s) 347, 707, 1310, 3103
AcuI CTGAAG 3 cut(s) 1662, 1829, 3257
AfaI GTAC 5 cut(s) 156, 324, 1823, 1983, 3341
AfiI CCNNNNNNNGG 4 cut(s) 512, 2408, 2632, 2701
AflII CTTAAG 1 cut(s) 680
AflIII ACRYGT 4 cut(s) 637, 857, 2259, 2828
AhdI GACNNNNNGTC 1 cut(s) 284
AjnI CCWGG 3 cut(s) 534, 789, 3254
AjuI GAANNNNNNNTTGG 4 cut(s) 2956, 2988, 3356, 3388
Alw21I GWGCWC 2 cut(s) 762, 910
Alw26I GTCTC 5 cut(s) 393, 513, 1080, 2510, 3194
AlwI GGATC 2 cut(s) 534, 1324
Ama87I CYCGRG 3 cut(s) 23, 3191, 3335
AoxI GGCC 2 cut(s) 591, 2960
ApeKI GCWGC 5 cut(s) 43, 305, 1121, 1505, 2324
ApoI RAATTY 4 cut(s) 347, 707, 1310, 3103
AseI ATTAAT 1 cut(s) 1953
Asp700I GAANNNNTTC 4 cut(s) 423, 776, 2341, 3371
Asp718I GGTACC 1 cut(s) 1821
AspA2I CCTAGG 1 cut(s) 62
AspLEI GCGC 2 cut(s) 939, 3262
AspS9I GGNCC 4 cut(s) 503, 876, 1753, 2090
AsuC2I CCSGG 1 cut(s) 2108
AsuHPI GGTGA 6 cut(s) 376, 940, 1175, 1616, 2284, 2324
AsuII TTCGAA 1 cut(s) 1259
AvaI CYCGRG 3 cut(s) 23, 3191, 3335
AvaII GGWCC 4 cut(s) 503, 876, 1753, 2090
AvrII CCTAGG 1 cut(s) 62
BaeI ACNNNNGTAYC 4 cut(s) 1796, 1829, 2296, 2329
BalI TGGCCA 2 cut(s) 593, 2962
BanI GGYRCC 2 cut(s) 1821, 2644
BanII GRGCYC 2 cut(s) 62, 3146
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 2 cut(s) 762, 910
BbvI GCAGC 5 cut(s) 30, 292, 1108, 1492, 2311
BccI CCATC 9 cut(s) 494, 581, 955, 1336, 1343, 1607, 2538, 2843, 3226
BceAI ACGGC 4 cut(s) 1099, 2242, 2315, 2485
BcgI CGANNNNNNTGC 6 cut(s) 551, 585, 641, 675, 1997, 2031
BciT130I CCWGG 3 cut(s) 536, 791, 3256
BciVI GTATCC 1 cut(s) 847
BcnI CCSGG 1 cut(s) 2108
BcoDI GTCTC 5 cut(s) 393, 513, 1080, 2510, 3194
BfaI CTAG 9 cut(s) 63, 450, 567, 911, 1425, 2012, 2352, 2969, 2988
BfmI CTRYAG 1 cut(s) 1506
BfoI RGCGCY 1 cut(s) 3263
BfrI CTTAAG 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 1006
BfuI GTATCC 1 cut(s) 847
BisI GCNGC 5 cut(s) 44, 306, 1122, 1506, 2325
BlnI CCTAGG 1 cut(s) 62
BlsI GCNGC 5 cut(s) 45, 307, 1123, 1507, 2326
Bme1390I CCNGG 4 cut(s) 536, 791, 2108, 3256
Bme18I GGWCC 4 cut(s) 503, 876, 1753, 2090
BmeRI GACNNNNNGTC 1 cut(s) 284
BmeT110I CYCGRG 3 cut(s) 23, 3191, 3335
BmgT120I GGNCC 4 cut(s) 503, 876, 1753, 2090
BmiI GGNNCC 6 cut(s) 329, 967, 1754, 1823, 2646, 3360
BmrFI CCNGG 4 cut(s) 536, 791, 2108, 3256
BmrI ACTGGG 2 cut(s) 532, 1220
BmsI GCATC 9 cut(s) 154, 649, 1262, 1372, 2110, 2146, 2751, 3086, 3301
BmuI ACTGGG 2 cut(s) 532, 1220
BoxI GACNNNNGTC 1 cut(s) 3298
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 6 cut(s) 1550, 2153, 2475, 2654, 3317, 3373
Bpu14I TTCGAA 1 cut(s) 1259
BpuEI CTTGAG 4 cut(s) 101, 1768, 2110, 2411
BpuMI CCSGG 1 cut(s) 2108
BsaI GGTCTC 1 cut(s) 2510
BsaJI CCNNGG 4 cut(s) 62, 2107, 3254, 3255
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 2 cut(s) 2088, 2118
Bsc4I CCNNNNNNNGG 4 cut(s) 512, 2408, 2632, 2701
Bse3DI GCAATG 2 cut(s) 1111, 2834
BseBI CCWGG 3 cut(s) 536, 791, 3256
BseDI CCNNGG 4 cut(s) 62, 2107, 3254, 3255
BseLI CCNNNNNNNGG 4 cut(s) 512, 2408, 2632, 2701
BseMI GCAATG 2 cut(s) 1111, 2834
BseMII CTCAG 5 cut(s) 814, 1229, 1981, 2466, 2941
BseRI GAGGAG 4 cut(s) 185, 2834, 3143, 3194
BseXI GCAGC 5 cut(s) 30, 292, 1108, 1492, 2311
Bsh1236I CGCG 1 cut(s) 937
Bsh1285I CGRYCG 1 cut(s) 497
BshFI GGCC 2 cut(s) 593, 2962
BshNI GGYRCC 2 cut(s) 1821, 2644
BsiEI CGRYCG 1 cut(s) 497
BsiHKAI GWGCWC 2 cut(s) 762, 910
BsiHKCI CYCGRG 3 cut(s) 23, 3191, 3335
BsiSI CCGG 2 cut(s) 497, 2107
BslFI GGGAC 2 cut(s) 1097, 2125
BslI CCNNNNNNNGG 4 cut(s) 512, 2408, 2632, 2701
BsmAI GTCTC 5 cut(s) 393, 513, 1080, 2510, 3194
BsmFI GGGAC 2 cut(s) 1097, 2125
BsmI GAATGC 2 cut(s) 29, 2004
BsnI GGCC 2 cut(s) 593, 2962
Bso31I GGTCTC 1 cut(s) 2510
BsoBI CYCGRG 3 cut(s) 23, 3191, 3335
Bsp119I TTCGAA 1 cut(s) 1259
Bsp1286I GDGCHC 4 cut(s) 62, 762, 910, 3146
Bsp1407I TGTACA 1 cut(s) 1981
Bsp143I GATC 6 cut(s) 69, 214, 381, 510, 526, 1329
BspACI CCGC 4 cut(s) 167, 1657, 2746, 3065
BspANI GGCC 2 cut(s) 593, 2962
BspCNI CTCAG 5 cut(s) 813, 1230, 1980, 2467, 2940
BspFNI CGCG 1 cut(s) 937
BspLI GGNNCC 6 cut(s) 329, 967, 1754, 1823, 2646, 3360
BspMAI CTGCAG 1 cut(s) 1510
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 2 cut(s) 534, 1324
BspQI GCTCTTC 2 cut(s) 2664, 3151
BspT104I TTCGAA 1 cut(s) 1259
BspT107I GGYRCC 2 cut(s) 1821, 2644
BspTI CTTAAG 1 cut(s) 680
BspTNI GGTCTC 1 cut(s) 2510
BsrDI GCAATG 2 cut(s) 1111, 2834
BsrGI TGTACA 1 cut(s) 1981
BssECI CCNNGG 4 cut(s) 62, 2107, 3254, 3255
BssMI GATC 6 cut(s) 69, 214, 381, 510, 526, 1329
BssNAI GTATAC 1 cut(s) 2767
BssT1I CCWWGG 1 cut(s) 62
Bst1107I GTATAC 1 cut(s) 2767
Bst2UI CCWGG 3 cut(s) 536, 791, 3256
Bst4CI ACNGT 9 cut(s) 672, 764, 1149, 1457, 1500, 1821, 2211, 2415, 3215
Bst6I CTCTTC 4 cut(s) 511, 1083, 2664, 3151
BstAFI CTTAAG 1 cut(s) 680
BstAUI TGTACA 1 cut(s) 1981
BstBI TTCGAA 1 cut(s) 1259
BstC8I GCNNGC 3 cut(s) 85, 2498, 2623
BstDEI CTNAG 8 cut(s) 250, 800, 1238, 1562, 1967, 2475, 2927, 3074
BstFNI CGCG 1 cut(s) 937
BstH2I RGCGCY 1 cut(s) 3263
BstHHI GCGC 2 cut(s) 939, 3262
BstKTI GATC 6 cut(s) 72, 217, 384, 513, 529, 1332
BstMAI GTCTC 5 cut(s) 393, 513, 1080, 2510, 3194
BstMBI GATC 6 cut(s) 69, 214, 381, 510, 526, 1329
BstMCI CGRYCG 1 cut(s) 497
BstMWI GCNNNNNNNGC 3 cut(s) 1502, 1738, 2165
BstNI CCWGG 3 cut(s) 536, 791, 3256
BstNSI RCATGY 6 cut(s) 641, 861, 1555, 2263, 2625, 2832
BstPAI GACNNNNGTC 1 cut(s) 3298
BstSCI CCNGG 4 cut(s) 534, 789, 2106, 3254
BstSFI CTRYAG 1 cut(s) 1506
BstUI CGCG 1 cut(s) 937
BstV1I GCAGC 5 cut(s) 30, 292, 1108, 1492, 2311
BstV2I GAAGAC 1 cut(s) 249
BstXI CCANNNNNNTGG 1 cut(s) 542
BstZ17I GTATAC 1 cut(s) 2767
BsuI GTATCC 1 cut(s) 847
BsuRI GGCC 2 cut(s) 593, 2962
BtsIMutI CAGTG 4 cut(s) 760, 1321, 2216, 2307
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 3 cut(s) 85, 2498, 2623
CfoI GCGC 2 cut(s) 939, 3262
Cfr13I GGNCC 4 cut(s) 503, 876, 1753, 2090
CseI GACGC 3 cut(s) 288, 1138, 1949
Csp6I GTAC 5 cut(s) 155, 323, 1822, 1982, 3340
CviQI GTAC 5 cut(s) 155, 323, 1822, 1982, 3340
DdeI CTNAG 8 cut(s) 250, 800, 1238, 1562, 1967, 2475, 2927, 3074
DpnI GATC 6 cut(s) 71, 216, 383, 512, 528, 1331
DpnII GATC 6 cut(s) 69, 214, 381, 510, 526, 1329
DriI GACNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 2 cut(s) 591, 2960
Eam1104I CTCTTC 4 cut(s) 511, 1083, 2664, 3151
Eam1105I GACNNNNNGTC 1 cut(s) 284
EarI CTCTTC 4 cut(s) 511, 1083, 2664, 3151
Eco130I CCWWGG 1 cut(s) 62
Eco24I GRGCYC 2 cut(s) 62, 3146
Eco31I GGTCTC 1 cut(s) 2510
Eco32I GATATC 2 cut(s) 3056, 3228
Eco47I GGWCC 4 cut(s) 503, 876, 1753, 2090
Eco57I CTGAAG 3 cut(s) 1662, 1829, 3257
Eco88I CYCGRG 3 cut(s) 23, 3191, 3335
EcoO109I RGGNCCY 2 cut(s) 1753, 2090
EcoRI GAATTC 2 cut(s) 707, 1310
EcoRII CCWGG 3 cut(s) 534, 789, 3254
EcoRV GATATC 2 cut(s) 3056, 3228
EcoT14I CCWWGG 1 cut(s) 62
EcoT22I ATGCAT 4 cut(s) 1476, 2623, 2744, 3316
EcoT38I GRGCYC 2 cut(s) 62, 3146
ErhI CCWWGG 1 cut(s) 62
FalI AAGNNNNNCTT 6 cut(s) 30, 62, 408, 440, 3356, 3388
FaqI GGGAC 2 cut(s) 1097, 2125
FauNDI CATATG 2 cut(s) 360, 3206
FblI GTMKAC 3 cut(s) 493, 1378, 2766
Fnu4HI GCNGC 5 cut(s) 44, 306, 1122, 1506, 2325
FriOI GRGCYC 2 cut(s) 62, 3146
Fsp4HI GCNGC 5 cut(s) 44, 306, 1122, 1506, 2325
FspBI CTAG 9 cut(s) 63, 450, 567, 911, 1425, 2012, 2352, 2969, 2988
GlaI GCGC 2 cut(s) 938, 3261
GluI GCNGC 5 cut(s) 44, 306, 1122, 1506, 2325
GsuI CTGGAG 6 cut(s) 1550, 2153, 2475, 2654, 3317, 3373
HaeII RGCGCY 1 cut(s) 3263
HaeIII GGCC 2 cut(s) 593, 2962
HapII CCGG 2 cut(s) 497, 2107
HgaI GACGC 3 cut(s) 288, 1138, 1949
HhaI GCGC 2 cut(s) 939, 3262
Hin6I GCGC 2 cut(s) 937, 3260
HinP1I GCGC 2 cut(s) 937, 3260
HincII GTYRAC 4 cut(s) 494, 813, 2022, 2782
HindII GTYRAC 4 cut(s) 494, 813, 2022, 2782
HindIII AAGCTT 1 cut(s) 2606
HpaI GTTAAC 1 cut(s) 2022
HpaII CCGG 2 cut(s) 497, 2107
HphI GGTGA 6 cut(s) 376, 940, 1175, 1616, 2284, 2324
Hpy99I CGWCG 1 cut(s) 282
HpyCH4III ACNGT 9 cut(s) 672, 764, 1149, 1457, 1500, 1821, 2211, 2415, 3215
HpyCH4IV ACGT 2 cut(s) 1651, 1661
HpyF10VI GCNNNNNNNGC 3 cut(s) 1502, 1738, 2165
HpyF3I CTNAG 8 cut(s) 250, 800, 1238, 1562, 1967, 2475, 2927, 3074
HpySE526I ACGT 2 cut(s) 1651, 1661
HspAI GCGC 2 cut(s) 937, 3260
KpnI GGTACC 1 cut(s) 1825
KspAI GTTAAC 1 cut(s) 2022
Kzo9I GATC 6 cut(s) 69, 214, 381, 510, 526, 1329
LguI GCTCTTC 2 cut(s) 2664, 3151
Lsp1109I GCAGC 5 cut(s) 30, 292, 1108, 1492, 2311
LweI GCATC 9 cut(s) 154, 649, 1262, 1372, 2110, 2146, 2751, 3086, 3301
MaeI CTAG 9 cut(s) 63, 450, 567, 911, 1425, 2012, 2352, 2969, 2988
MaeII ACGT 2 cut(s) 1651, 1661
MalI GATC 6 cut(s) 71, 216, 383, 512, 528, 1331
MboI GATC 6 cut(s) 69, 214, 381, 510, 526, 1329
MfeI CAATTG 2 cut(s) 2709, 2915
MhlI GDGCHC 4 cut(s) 62, 762, 910, 3146
MlsI TGGCCA 2 cut(s) 593, 2962
MluNI TGGCCA 2 cut(s) 593, 2962
MlyI GAGTC 8 cut(s) 294, 387, 1082, 1207, 2078, 3191, 3252, 3308
MmeI TCCRAC 3 cut(s) 523, 595, 883
Mox20I TGGCCA 2 cut(s) 593, 2962
Mph1103I ATGCAT 4 cut(s) 1476, 2623, 2744, 3316
MroXI GAANNNNTTC 4 cut(s) 423, 776, 2341, 3371
MscI TGGCCA 2 cut(s) 593, 2962
MseI TTAA 7 cut(s) 681, 1047, 1953, 2021, 2174, 3006, 3264
MslI CAYNNNNRTG 2 cut(s) 1937, 2354
Msp20I TGGCCA 2 cut(s) 593, 2962
MspCI CTTAAG 1 cut(s) 680
MspI CCGG 2 cut(s) 497, 2107
MspR9I CCNGG 4 cut(s) 536, 791, 2108, 3256
MunI CAATTG 2 cut(s) 2709, 2915
Mva1269I GAATGC 2 cut(s) 29, 2004
MvaI CCWGG 3 cut(s) 536, 791, 3256
MvnI CGCG 1 cut(s) 937
MwoI GCNNNNNNNGC 3 cut(s) 1502, 1738, 2165
NciI CCSGG 1 cut(s) 2108
NdeI CATATG 2 cut(s) 360, 3206
NdeII GATC 6 cut(s) 69, 214, 381, 510, 526, 1329
NlaIV GGNNCC 6 cut(s) 329, 967, 1754, 1823, 2646, 3360
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 6 cut(s) 724, 860, 928, 2140, 2690, 2871
NsiI ATGCAT 4 cut(s) 1476, 2623, 2744, 3316
NspI RCATGY 6 cut(s) 641, 861, 1555, 2263, 2625, 2832
NspV TTCGAA 1 cut(s) 1259
PaeI GCATGC 1 cut(s) 2625
PaeR7I CTCGAG 2 cut(s) 3191, 3335
PasI CCCWGGG 1 cut(s) 3255
PciI ACATGT 4 cut(s) 637, 857, 2259, 2828
PciSI GCTCTTC 2 cut(s) 2664, 3151
PctI GAATGC 2 cut(s) 29, 2004
PdmI GAANNNNTTC 4 cut(s) 423, 776, 2341, 3371
PflMI CCANNNNNTGG 2 cut(s) 2408, 2701
PfoI TCCNGGA 1 cut(s) 789
PkrI GCNGC 5 cut(s) 45, 307, 1123, 1507, 2326
PleI GAGTC 8 cut(s) 293, 387, 1081, 1206, 2077, 3190, 3251, 3307
PpsI GAGTC 8 cut(s) 293, 387, 1081, 1206, 2077, 3190, 3251, 3307
PpuMI RGGWCCY 2 cut(s) 1753, 2090
PscI ACATGT 4 cut(s) 637, 857, 2259, 2828
PshAI GACNNNNGTC 1 cut(s) 3298
PshBI ATTAAT 1 cut(s) 1953
PsiI TTATAA 2 cut(s) 1881, 2441
Psp1406I AACGTT 1 cut(s) 1651
Psp5II RGGWCCY 2 cut(s) 1753, 2090
Psp6I CCWGG 3 cut(s) 534, 789, 3254
PspGI CCWGG 3 cut(s) 534, 789, 3254
PspN4I GGNNCC 6 cut(s) 329, 967, 1754, 1823, 2646, 3360
PspPI GGNCC 4 cut(s) 503, 876, 1753, 2090
PspPPI RGGWCCY 2 cut(s) 1753, 2090
PsrI GAACNNNNNNTAC 2 cut(s) 2280, 2312
PstI CTGCAG 1 cut(s) 1510
RsaI GTAC 5 cut(s) 156, 324, 1823, 1983, 3341
RsaNI GTAC 5 cut(s) 155, 323, 1822, 1982, 3340
RseI CAYNNNNRTG 2 cut(s) 1937, 2354
SalI GTCGAC 1 cut(s) 492
SapI GCTCTTC 2 cut(s) 2664, 3151
SaqAI TTAA 7 cut(s) 681, 1047, 1953, 2021, 2174, 3006, 3264
SatI GCNGC 5 cut(s) 44, 306, 1122, 1506, 2325
Sau3AI GATC 6 cut(s) 69, 214, 381, 510, 526, 1329
Sau96I GGNCC 4 cut(s) 503, 876, 1753, 2090
SchI GAGTC 8 cut(s) 294, 387, 1082, 1207, 2078, 3191, 3252, 3308
ScrFI CCNGG 4 cut(s) 536, 791, 2108, 3256
SduI GDGCHC 4 cut(s) 62, 762, 910, 3146
SfaNI GCATC 9 cut(s) 154, 649, 1262, 1372, 2110, 2146, 2751, 3086, 3301
SfcI CTRYAG 1 cut(s) 1506
Sfr274I CTCGAG 2 cut(s) 3191, 3335
SfuI TTCGAA 1 cut(s) 1259
SinI GGWCC 4 cut(s) 503, 876, 1753, 2090
SlaI CTCGAG 2 cut(s) 3191, 3335
SmiMI CAYNNNNRTG 2 cut(s) 1937, 2354
SmlI CTYRAG 8 cut(s) 116, 680, 1747, 2125, 2426, 3191, 3335, 3380
SmoI CTYRAG 8 cut(s) 116, 680, 1747, 2125, 2426, 3191, 3335, 3380
SphI GCATGC 1 cut(s) 2625
SsiI CCGC 4 cut(s) 167, 1657, 2746, 3065
SspI AATATT 1 cut(s) 1179
SspMI CTAG 9 cut(s) 63, 450, 567, 911, 1425, 2012, 2352, 2969, 2988
StyD4I CCNGG 4 cut(s) 534, 789, 2106, 3254
StyI CCWWGG 1 cut(s) 62
TaaI ACNGT 9 cut(s) 672, 764, 1149, 1457, 1500, 1821, 2211, 2415, 3215
TaiI ACGT 2 cut(s) 1654, 1664
TaqI TCGA 8 cut(s) 267, 493, 741, 1259, 1582, 3022, 3192, 3336
TatI WGTACW 3 cut(s) 154, 1981, 3339
Tru1I TTAA 7 cut(s) 681, 1047, 1953, 2021, 2174, 3006, 3264
Tru9I TTAA 7 cut(s) 681, 1047, 1953, 2021, 2174, 3006, 3264
TscAI CASTG 4 cut(s) 767, 1321, 2216, 2314
TseFI GTSAC 6 cut(s) 724, 860, 928, 2140, 2690, 2871
TseI GCWGC 5 cut(s) 43, 305, 1121, 1505, 2324
Tsp45I GTSAC 6 cut(s) 724, 860, 928, 2140, 2690, 2871
TspGWI ACGGA 4 cut(s) 340, 544, 887, 2722
TspRI CASTG 4 cut(s) 767, 1321, 2216, 2314
Van91I CCANNNNNTGG 2 cut(s) 2408, 2701
Vha464I CTTAAG 1 cut(s) 680
VpaK11BI GGWCC 4 cut(s) 503, 876, 1753, 2090
VspI ATTAAT 1 cut(s) 1953
XapI RAATTY 4 cut(s) 347, 707, 1310, 3103
XceI RCATGY 6 cut(s) 641, 861, 1555, 2263, 2625, 2832
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XhoI CTCGAG 2 cut(s) 3191, 3335
XmaJI CCTAGG 1 cut(s) 62
XmiI GTMKAC 3 cut(s) 493, 1378, 2766
XmnI GAANNNNTTC 4 cut(s) 423, 776, 2341, 3371
XspI CTAG 9 cut(s) 63, 450, 567, 911, 1425, 2012, 2352, 2969, 2988
Zsp2I ATGCAT 4 cut(s) 1476, 2623, 2744, 3316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.