Rw1G003670

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000070
Physical Location & Seq
Reverse (-)
6795510 .. 6798137
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003670.1

Sequence Viewer

Length: 2628 bp
ATGGAAGATATCGGTGATGAATATTTTGGGGAGCTACTATCTCGGTCATTATTTCAAAACTCGGGCAAAAACAGTTCATGCTATGTAATGCATGACCTTGTTGGTGAGTTGGCACGATGGGCTGCTGGCGACACATTTTGCAGATTGGAGGATAAACTGGATGGTAGATGTTCTGCAAAGACTCGTCATCTGGCTTACATTTCTGGTAAGTTCGATGGGGTAAAAAGATTTGAGACAATTTCTGGAGCAAAACGCTTGCGGACTTTCCAACCATTTCCCGTTTCTGGTGGTTATGAGAATTATCTAACTCGTTATGCTACTTCTGATTTATTGCCACAATTGAAGTACTTGCGGGTGCTCTCCTTTAATGGCTATAAACTGACCGAGCTGCCAGATTCTGTAGGTAAGTTGAAACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGAGTTTGCCTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAACTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTGTCTAATTTACGTCATCTCAACAATTCAAACACGCCTTCATTGGAAGAAATGCCTCCCCAAGTAAGTCGGTTGACTCATCTGCAAACTGTGCTTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGCTTGGAGAGATAGGGTCATTGCATCTTCGTGGGACCATAAGCCTTGGAAGATTGGAAAATGTGATCGATGTTGGGGATGTGAAGAGGGCCAAAATAATGAATAAGGAAGGGCTGGATACATTGCAATTGGAATGGAGTGGCACAAGTGAGAAGGAGTCGGAGGTGCTCTGCAGTTTAGAACCTCATAAAAAGCTTCTAGACCTCACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGCATGTGAAGTTAGAGAATTGTAAAAATTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAGGACTTTGGATACAAGGACTGGCCAATGTTAAAAGTGTGGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGATGGAAGACCTGAGGTTCGAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAACAAATGAAGAAATTCGAGTTTTCCCTTGCCTGCGACAGGTTTCCATCTCTAGATGCCCGAAATTGAAGTGTTTGCTATCCAAGAGCATGGATTCATTATCAGAGCTGTATATTGATGAATGTGAAGAGTTGGTGGTTTCAATTGCCAATTATAAACATGTAAGTGTGTTGGTCATAACAGGATGTAAAAGGGTGGTGCACAGAAGCGGAGTTAAGTTTGAGTCACTGGAGGTTATGGCGCTTGATAATATTTCAGAGTTCAGACTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAAATTTGTGGATTACTGGTTGTGAAGAGTTGACATGTTTATGGGAGAATGAGGATAGATGGCTGCAGCGTCGGATTTCTAATATTATTGGAGGCAACATATCTGACTCCCCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAAGGAACTTGTGCTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGCTAGCTGGCCACCTTGTCTTGAATTTGTAAAGATGGCGTCGTGTAATTCTTTGACATATTTCGTAAGGTATCAAGTACCGCCAAGCATAAGAACAATAGAGATAGAAAAATGTCAAAATTTGAAACTATTAGTGAAGGATGCTGACAAAGGAGGCCGACGGCTCAAATATCTTTATTTAAGTGACTGTGAACGATTGGAGTCAATAATAAAAACTTTCCACGAGGACACTTGTCTTGAAGATCTTAGGATATACAGGTGTGCAAATCTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCGGCTCTTCGCATTTTAATTATACGCGATTGTGGAAGTCTCGTTTCCTTCCCAAGAGGAGGGTTGCCATCCAACCTGATATACTTTCATATGGACGATTGTGATCAATTGGAAGCCCTCCCCAGAGGCATGGACAACCTCAGCTCTCTTCAGACTTTGGTGTTGAGACACTGTGGAGGTTTGGCATCCATTATAGAAGAGGGTTTCCCACCCAACCTAATTGATCTGGAAATTGGCAATTGCAAAAGCTGCAAGCCTCTCTCAGAGTGGGGGCTTCACCACTTGGACAGACTCACCTCTCTTAAAGTCTTGTGGATCGGTGGTGTAGATCCAGATCTGGTGTCCTTTCCTCCAAAGGAGGTGCTGCTCCCTAAATCTCTCATTCAACTCGTGATTGGAAGGTTCCCAAATCTGAAGCGCCTGTCATCATCTTTTCAATCACTCACCTCTCTTGAATCCCTTGAAATTGGTAATTGCCCAAAGCTAGCATCTATTATTCCAGAAAAAGAGGATCATCTGCCTCTTTCTCTCACACAGCTTCGCATCCGTGAGAGCTGTCCGCTGCTCACAAAGAAATACAAACCAGGTAAAGGACGACACTGGCCCAAAGAAATAGCCCACATCCCTTACGTTTTTGTTGGAGACTGGGAGGACGAAGCCGAAGCCGACCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

875

Amino Acids

99.01

Weight (kDa)

6.64

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 1 - 38 4.1e-09 Disease resistance protein Winged helix domain
LRR_14 PF23598 111 - 378 4.3e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 228 - 346 1.3e-30 R13L1/DRL21 LRRs
LRR_14 PF23598 606 - 807 3.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1311
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 423
AccII CGCG 1 cut(s) 1980
AciI CCGC 6 cut(s) 259, 352, 1365, 1733, 2513, 2623
AclWI GGATC 3 cut(s) 2276, 2276, 2472
AcoI YGGCCR 3 cut(s) 1000, 1041, 1661
AcsI RAATTY 5 cut(s) 908, 1170, 1465, 1676, 1771
AcuI CTGAAG 2 cut(s) 2087, 2387
AcyI GRCGYC 1 cut(s) 1691
AfaI GTAC 3 cut(s) 347, 425, 1731
AfiI CCNNNNNNNGG 6 cut(s) 284, 998, 1195, 1954, 2012, 2543
AflIII ACRYGT 2 cut(s) 1315, 1496
AjnI CCWGG 1 cut(s) 2536
AjuI GAANNNNNNNTTGG 2 cut(s) 996, 1028
Alw21I GWGCWC 4 cut(s) 360, 846, 1359, 1603
Alw26I GTCTC 4 cut(s) 227, 1997, 2113, 2589
Alw44I GTGCAC 1 cut(s) 1355
AlwI GGATC 3 cut(s) 2276, 2276, 2472
AlwNI CAGNNNCTG 1 cut(s) 398
Ama87I CYCGRG 1 cut(s) 61
AoxI GGCC 6 cut(s) 765, 1000, 1041, 1661, 1807, 2555
ApaLI GTGCAC 1 cut(s) 1355
ApeKI GCWGC 8 cut(s) 122, 388, 1083, 1525, 1528, 2202, 2317, 2515
ApoI RAATTY 5 cut(s) 908, 1170, 1465, 1676, 1771
ArsI GACNNNNNNTTYG 2 cut(s) 1100, 1132
AseI ATTAAT 1 cut(s) 446
Asp700I GAANNNNTTC 1 cut(s) 1170
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 2 cut(s) 1399, 2373
AspS9I GGNCC 3 cut(s) 711, 765, 2556
AsuHPI GGTGA 6 cut(s) 26, 116, 874, 2222, 2239, 2389
AsuII TTCGAA 1 cut(s) 906
AsuNHI GCTAGC 2 cut(s) 1655, 2437
AvaI CYCGRG 1 cut(s) 61
AvaII GGWCC 1 cut(s) 711
AxyI CCTNAGG 1 cut(s) 1112
BaeGI GKGCMC 1 cut(s) 1359
BalI TGGCCA 3 cut(s) 1002, 1043, 1663
BanI GGYRCC 1 cut(s) 423
BauI CACGAG 2 cut(s) 1874, 2342
BbsI GAAGAC 2 cut(s) 530, 1113
Bbv12I GWGCWC 4 cut(s) 360, 846, 1359, 1603
BbvCI CCTCAGC 1 cut(s) 2093
BbvI GCAGC 8 cut(s) 109, 375, 1095, 1512, 1540, 2189, 2304, 2502
BceAI ACGGC 1 cut(s) 1829
BciT130I CCWGG 1 cut(s) 2538
BciVI GTATCC 2 cut(s) 787, 1023
BclI TGATCA 1 cut(s) 2056
BcoDI GTCTC 4 cut(s) 227, 1997, 2113, 2589
BfaI CTAG 4 cut(s) 875, 1209, 1656, 2438
BfmI CTRYAG 3 cut(s) 399, 847, 1526
BfoI RGCGCY 2 cut(s) 1400, 2374
BfuI GTATCC 2 cut(s) 787, 1023
BglII AGATCT 2 cut(s) 1894, 2287
BisI GCNGC 8 cut(s) 123, 389, 1084, 1526, 1529, 2203, 2318, 2516
BlsI GCNGC 8 cut(s) 124, 390, 1085, 1527, 1530, 2204, 2319, 2517
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 1 cut(s) 2538
Bme18I GGWCC 1 cut(s) 711
BmeT110I CYCGRG 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 711, 765, 2556
BmiI GGNNCC 3 cut(s) 425, 712, 2357
BmrFI CCNGG 1 cut(s) 2538
BmrI ACTGGG 1 cut(s) 2608
BmsI GCATC 8 cut(s) 680, 709, 1202, 1616, 1783, 2147, 2450, 2505
BmtI GCTAGC 2 cut(s) 1659, 2441
BmuI ACTGGG 1 cut(s) 2608
BoxI GACNNNNGTC 1 cut(s) 1884
BpiI GAAGAC 2 cut(s) 530, 1113
BpmI CTGGAG 2 cut(s) 264, 1406
Bpu10I CCTNAGC 1 cut(s) 2093
Bpu14I TTCGAA 1 cut(s) 906
BpuEI CTTGAG 1 cut(s) 1589
Bsa29I ATCGAT 1 cut(s) 744
BsaBI GATNNNNATC 2 cut(s) 2055, 2286
BsaHI GRCGYC 1 cut(s) 1691
BsaJI CCNNGG 1 cut(s) 721
Bsc4I CCNNNNNNNGG 6 cut(s) 284, 998, 1195, 1954, 2012, 2543
Bse1I ACTGG 8 cut(s) 162, 1044, 1097, 1389, 1483, 1627, 2558, 2603
Bse21I CCTNAGG 1 cut(s) 1112
Bse3DI GCAATG 2 cut(s) 695, 797
Bse8I GATNNNNATC 2 cut(s) 2055, 2286
BseBI CCWGG 1 cut(s) 2538
BseCI ATCGAT 1 cut(s) 744
BseDI CCNNGG 1 cut(s) 721
BseJI GATNNNNATC 2 cut(s) 2055, 2286
BseLI CCNNNNNNNGG 6 cut(s) 284, 998, 1195, 1954, 2012, 2543
BseMI GCAATG 2 cut(s) 695, 797
BseMII CTCAG 5 cut(s) 453, 1056, 1103, 2107, 2229
BseNI ACTGG 8 cut(s) 162, 1044, 1097, 1389, 1483, 1627, 2558, 2603
BseRI GAGGAG 2 cut(s) 984, 2025
BseSI GKGCMC 1 cut(s) 1359
BseXI GCAGC 8 cut(s) 109, 375, 1095, 1512, 1540, 2189, 2304, 2502
BsgI GTGCAG 1 cut(s) 1102
Bsh1236I CGCG 1 cut(s) 1980
Bsh1285I CGRYCG 1 cut(s) 2623
BshFI GGCC 6 cut(s) 767, 1002, 1043, 1663, 1809, 2557
BshNI GGYRCC 1 cut(s) 423
BshVI ATCGAT 1 cut(s) 744
BsiEI CGRYCG 1 cut(s) 2623
BsiHKAI GWGCWC 4 cut(s) 360, 846, 1359, 1603
BsiHKCI CYCGRG 1 cut(s) 61
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 6 cut(s) 284, 998, 1195, 1954, 2012, 2543
BsmAI GTCTC 4 cut(s) 227, 1997, 2113, 2589
BsmFI GGGAC 1 cut(s) 724
BsnI GGCC 6 cut(s) 767, 1002, 1043, 1663, 1809, 2557
BsoBI CYCGRG 1 cut(s) 61
Bsp119I TTCGAA 1 cut(s) 906
Bsp1286I GDGCHC 4 cut(s) 360, 846, 1359, 1603
Bsp143I GATC 9 cut(s) 430, 741, 1894, 2056, 2176, 2268, 2281, 2287, 2464
BspACI CCGC 6 cut(s) 259, 352, 1365, 1733, 2513, 2623
BspANI GGCC 6 cut(s) 767, 1002, 1043, 1663, 1809, 2557
BspCNI CTCAG 5 cut(s) 454, 1057, 1104, 2106, 2228
BspDI ATCGAT 1 cut(s) 744
BspFNI CGCG 1 cut(s) 1980
BspLI GGNNCC 3 cut(s) 425, 712, 2357
BspMAI CTGCAG 2 cut(s) 851, 1530
BspOI GCTAGC 2 cut(s) 1659, 2441
BspPI GGATC 3 cut(s) 2276, 2276, 2472
BspQI GCTCTTC 1 cut(s) 1965
BspT104I TTCGAA 1 cut(s) 906
BspT107I GGYRCC 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 695, 797
BsrI ACTGG 8 cut(s) 162, 1044, 1097, 1389, 1483, 1627, 2558, 2603
BssECI CCNNGG 1 cut(s) 721
BssMI GATC 9 cut(s) 430, 741, 1894, 2056, 2176, 2268, 2281, 2287, 2464
BssNI GRCGYC 1 cut(s) 1691
BssSI CACGAG 2 cut(s) 1874, 2342
BssT1I CCWWGG 1 cut(s) 721
Bst2BI CACGAG 2 cut(s) 1874, 2342
Bst2UI CCWGG 1 cut(s) 2538
Bst4CI ACNGT 6 cut(s) 74, 423, 512, 640, 1841, 2126
Bst6I CTCTTC 6 cut(s) 755, 1278, 1482, 1965, 2106, 2145
BstACI GRCGYC 1 cut(s) 1691
BstAPI GCANNNNNTGC 2 cut(s) 640, 2202
BstBI TTCGAA 1 cut(s) 906
BstC8I GCNNGC 9 cut(s) 127, 257, 476, 1088, 1190, 1657, 1661, 2207, 2439
BstDEI CTNAG 7 cut(s) 462, 1065, 1112, 1595, 1898, 2093, 2215
BstENI CCTNNNNNAGG 1 cut(s) 1193
BstFNI CGCG 1 cut(s) 1980
BstH2I RGCGCY 2 cut(s) 1400, 2374
BstHHI GCGC 2 cut(s) 1399, 2373
BstKTI GATC 9 cut(s) 433, 744, 1897, 2059, 2179, 2271, 2284, 2290, 2467
BstMAI GTCTC 4 cut(s) 227, 1997, 2113, 2589
BstMBI GATC 9 cut(s) 430, 741, 1894, 2056, 2176, 2268, 2281, 2287, 2464
BstMCI CGRYCG 1 cut(s) 2623
BstMWI GCNNNNNNNGC 3 cut(s) 119, 640, 2202
BstNI CCWGG 1 cut(s) 2538
BstNSI RCATGY 5 cut(s) 474, 478, 956, 1319, 1500
BstPAI GACNNNNGTC 1 cut(s) 1884
BstSCI CCNGG 1 cut(s) 2536
BstSFI CTRYAG 3 cut(s) 399, 847, 1526
BstSLI GKGCMC 1 cut(s) 1359
BstUI CGCG 1 cut(s) 1980
BstV1I GCAGC 8 cut(s) 109, 375, 1095, 1512, 1540, 2189, 2304, 2502
BstV2I GAAGAC 2 cut(s) 530, 1113
BstX2I RGATCY 3 cut(s) 1894, 2281, 2287
BstXI CCANNNNNNTGG 2 cut(s) 1246, 2083
BstYI RGATCY 3 cut(s) 1894, 2281, 2287
Bsu15I ATCGAT 1 cut(s) 744
Bsu36I CCTNAGG 1 cut(s) 1112
BsuI GTATCC 2 cut(s) 787, 1023
BsuRI GGCC 6 cut(s) 767, 1002, 1043, 1663, 1809, 2557
BsuTUI ATCGAT 1 cut(s) 744
BtsIMutI CAGTG 4 cut(s) 1104, 1382, 2122, 2551
Cac8I GCNNGC 9 cut(s) 127, 257, 476, 1088, 1190, 1657, 1661, 2207, 2439
CaiI CAGNNNCTG 1 cut(s) 398
CfoI GCGC 2 cut(s) 1399, 2373
Cfr13I GGNCC 3 cut(s) 711, 765, 2556
ClaI ATCGAT 1 cut(s) 744
CseI GACGC 3 cut(s) 958, 1520, 1680
CsiI ACCWGGT 1 cut(s) 2536
Csp6I GTAC 3 cut(s) 346, 424, 1730
CspCI CAANNNNNGTGG 2 cut(s) 1451, 1486
CviQI GTAC 3 cut(s) 346, 424, 1730
DdeI CTNAG 7 cut(s) 462, 1065, 1112, 1595, 1898, 2093, 2215
DpnI GATC 9 cut(s) 432, 743, 1896, 2058, 2178, 2270, 2283, 2289, 2466
DpnII GATC 9 cut(s) 430, 741, 1894, 2056, 2176, 2268, 2281, 2287, 2464
EaeI YGGCCR 3 cut(s) 1000, 1041, 1661
Eam1104I CTCTTC 6 cut(s) 755, 1278, 1482, 1965, 2106, 2145
EarI CTCTTC 6 cut(s) 755, 1278, 1482, 1965, 2106, 2145
Eco130I CCWWGG 1 cut(s) 721
Eco32I GATATC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 711
Eco57I CTGAAG 2 cut(s) 2087, 2387
Eco81I CCTNAGG 1 cut(s) 1112
Eco88I CYCGRG 1 cut(s) 61
EcoNI CCTNNNNNAGG 1 cut(s) 1193
EcoRII CCWGG 1 cut(s) 2536
EcoRV GATATC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 721
EcoT22I ATGCAT 2 cut(s) 93, 476
ErhI CCWWGG 1 cut(s) 721
FaqI GGGAC 1 cut(s) 724
FauI CCCGC 1 cut(s) 345
FauNDI CATATG 1 cut(s) 2043
FbaI TGATCA 1 cut(s) 2056
Fnu4HI GCNGC 8 cut(s) 123, 389, 1084, 1526, 1529, 2203, 2318, 2516
Fsp4HI GCNGC 8 cut(s) 123, 389, 1084, 1526, 1529, 2203, 2318, 2516
FspBI CTAG 4 cut(s) 875, 1209, 1656, 2438
GlaI GCGC 2 cut(s) 1398, 2372
GluI GCNGC 8 cut(s) 123, 389, 1084, 1526, 1529, 2203, 2318, 2516
GsuI CTGGAG 2 cut(s) 264, 1406
HaeII RGCGCY 2 cut(s) 1400, 2374
HaeIII GGCC 6 cut(s) 767, 1002, 1043, 1663, 1809, 2557
HgaI GACGC 3 cut(s) 958, 1520, 1680
HhaI GCGC 2 cut(s) 1399, 2373
Hin1I GRCGYC 1 cut(s) 1691
Hin6I GCGC 2 cut(s) 1397, 2371
HinP1I GCGC 2 cut(s) 1397, 2371
HincII GTYRAC 3 cut(s) 468, 624, 1494
HindII GTYRAC 3 cut(s) 468, 624, 1494
HindIII AAGCTT 2 cut(s) 869, 1457
HphI GGTGA 6 cut(s) 26, 116, 874, 2222, 2239, 2389
Hpy166II GTNNAC 5 cut(s) 468, 624, 1357, 1494, 1844
Hpy8I GTNNAC 5 cut(s) 468, 624, 1357, 1494, 1844
Hpy99I CGWCG 3 cut(s) 1536, 1696, 1815
HpyAV CCTTC 9 cut(s) 543, 597, 779, 823, 942, 1132, 1783, 2011, 2346
HpyCH4III ACNGT 6 cut(s) 74, 423, 512, 640, 1841, 2126
HpyCH4IV ACGT 3 cut(s) 494, 562, 2583
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 640, 2202
HpyF3I CTNAG 7 cut(s) 462, 1065, 1112, 1595, 1898, 2093, 2215
HpySE526I ACGT 3 cut(s) 494, 562, 2583
Hsp92I GRCGYC 1 cut(s) 1691
HspAI GCGC 2 cut(s) 1397, 2371
KpnI GGTACC 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 2056
Kzo9I GATC 9 cut(s) 430, 741, 1894, 2056, 2176, 2268, 2281, 2287, 2464
LguI GCTCTTC 1 cut(s) 1965
LmnI GCTCC 3 cut(s) 31, 245, 2325
Lsp1109I GCAGC 8 cut(s) 109, 375, 1095, 1512, 1540, 2189, 2304, 2502
LweI GCATC 8 cut(s) 680, 709, 1202, 1616, 1783, 2147, 2450, 2505
MabI ACCWGGT 1 cut(s) 2536
MaeI CTAG 4 cut(s) 875, 1209, 1656, 2438
MaeII ACGT 3 cut(s) 494, 562, 2583
MaeIII GTNAC 2 cut(s) 1380, 1835
MalI GATC 9 cut(s) 432, 743, 1896, 2058, 2178, 2270, 2283, 2289, 2466
MboI GATC 9 cut(s) 430, 741, 1894, 2056, 2176, 2268, 2281, 2287, 2464
MfeI CAATTG 5 cut(s) 338, 803, 1299, 2060, 2191
MflI RGATCY 3 cut(s) 1894, 2281, 2287
MhlI GDGCHC 4 cut(s) 360, 846, 1359, 1603
MlsI TGGCCA 3 cut(s) 1002, 1043, 1663
MluNI TGGCCA 3 cut(s) 1002, 1043, 1663
MlyI GAGTC 9 cut(s) 175, 473, 619, 842, 1388, 1416, 1562, 1862, 2238
MmeI TCCRAC 5 cut(s) 292, 816, 1514, 2049, 2572
Mox20I TGGCCA 3 cut(s) 1002, 1043, 1663
Mph1103I ATGCAT 2 cut(s) 93, 476
MroXI GAANNNNTTC 1 cut(s) 1170
MscI TGGCCA 3 cut(s) 1002, 1043, 1663
MseI TTAA 9 cut(s) 366, 446, 497, 645, 1050, 1371, 1832, 1970, 2256
MslI CAYNNNNRTG 3 cut(s) 705, 1320, 1501
Msp20I TGGCCA 3 cut(s) 1002, 1043, 1663
MspA1I CMGCKG 2 cut(s) 2515, 2625
MspR9I CCNGG 1 cut(s) 2538
MunI CAATTG 5 cut(s) 338, 803, 1299, 2060, 2191
MvaI CCWGG 1 cut(s) 2538
MvnI CGCG 1 cut(s) 1980
MwoI GCNNNNNNNGC 3 cut(s) 119, 640, 2202
NdeI CATATG 1 cut(s) 2043
NdeII GATC 9 cut(s) 430, 741, 1894, 2056, 2176, 2268, 2281, 2287, 2464
NheI GCTAGC 2 cut(s) 1655, 2437
NlaIV GGNNCC 3 cut(s) 425, 712, 2357
NmeAIII GCCGAG 1 cut(s) 1934
NmuCI GTSAC 2 cut(s) 1380, 1835
NsiI ATGCAT 2 cut(s) 93, 476
NspI RCATGY 5 cut(s) 474, 478, 956, 1319, 1500
NspV TTCGAA 1 cut(s) 906
PaeI GCATGC 1 cut(s) 478
PciI ACATGT 2 cut(s) 1315, 1496
PciSI GCTCTTC 1 cut(s) 1965
PdmI GAANNNNTTC 1 cut(s) 1170
PfeI GAWTC 4 cut(s) 395, 984, 1250, 2408
PkrI GCNGC 8 cut(s) 124, 390, 1085, 1527, 1530, 2204, 2319, 2517
PleI GAGTC 9 cut(s) 175, 472, 619, 841, 1387, 1416, 1562, 1861, 2238
PpsI GAGTC 9 cut(s) 175, 472, 619, 841, 1387, 1416, 1562, 1861, 2238
PscI ACATGT 2 cut(s) 1315, 1496
PshAI GACNNNNGTC 1 cut(s) 1884
PshBI ATTAAT 1 cut(s) 446
PsiI TTATAA 1 cut(s) 1311
Psp6I CCWGG 1 cut(s) 2536
PspGI CCWGG 1 cut(s) 2536
PspN4I GGNNCC 3 cut(s) 425, 712, 2357
PspPI GGNCC 3 cut(s) 711, 765, 2556
PstI CTGCAG 2 cut(s) 851, 1530
PstNI CAGNNNCTG 1 cut(s) 398
PsuI RGATCY 3 cut(s) 1894, 2281, 2287
RsaI GTAC 3 cut(s) 347, 425, 1731
RsaNI GTAC 3 cut(s) 346, 424, 1730
RseI CAYNNNNRTG 3 cut(s) 705, 1320, 1501
SapI GCTCTTC 1 cut(s) 1965
SaqAI TTAA 9 cut(s) 366, 446, 497, 645, 1050, 1371, 1832, 1970, 2256
SatI GCNGC 8 cut(s) 123, 389, 1084, 1526, 1529, 2203, 2318, 2516
Sau3AI GATC 9 cut(s) 430, 741, 1894, 2056, 2176, 2268, 2281, 2287, 2464
Sau96I GGNCC 3 cut(s) 711, 765, 2556
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 9 cut(s) 175, 473, 619, 842, 1388, 1416, 1562, 1862, 2238
ScrFI CCNGG 1 cut(s) 2538
SduI GDGCHC 4 cut(s) 360, 846, 1359, 1603
SexAI ACCWGGT 1 cut(s) 2536
SfaNI GCATC 8 cut(s) 680, 709, 1202, 1616, 1783, 2147, 2450, 2505
SfcI CTRYAG 3 cut(s) 399, 847, 1526
SfuI TTCGAA 1 cut(s) 906
SinI GGWCC 1 cut(s) 711
SmiMI CAYNNNNRTG 3 cut(s) 705, 1320, 1501
SmlI CTYRAG 1 cut(s) 1604
SmoI CTYRAG 1 cut(s) 1604
SphI GCATGC 1 cut(s) 478
SsiI CCGC 6 cut(s) 259, 352, 1365, 1733, 2513, 2623
SspI AATATT 4 cut(s) 23, 501, 1408, 1546
SspMI CTAG 4 cut(s) 875, 1209, 1656, 2438
StyD4I CCNGG 1 cut(s) 2536
StyI CCWWGG 1 cut(s) 721
TaaI ACNGT 6 cut(s) 74, 423, 512, 640, 1841, 2126
TaiI ACGT 3 cut(s) 497, 565, 2586
TaqI TCGA 7 cut(s) 213, 744, 906, 982, 1119, 1174, 1425
TaqII GACCGA 2 cut(s) 33, 398
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 4 cut(s) 395, 984, 1250, 2408
Tru1I TTAA 9 cut(s) 366, 446, 497, 645, 1050, 1371, 1832, 1970, 2256
Tru9I TTAA 9 cut(s) 366, 446, 497, 645, 1050, 1371, 1832, 1970, 2256
TscAI CASTG 4 cut(s) 1104, 1389, 2129, 2558
TseFI GTSAC 2 cut(s) 1380, 1835
TseI GCWGC 8 cut(s) 122, 388, 1083, 1525, 1528, 2202, 2317, 2515
Tsp45I GTSAC 2 cut(s) 1380, 1835
TspGWI ACGGA 1 cut(s) 2489
TspRI CASTG 4 cut(s) 1104, 1389, 2129, 2558
VneI GTGCAC 1 cut(s) 1355
VpaK11BI GGWCC 1 cut(s) 711
VspI ATTAAT 1 cut(s) 446
XagI CCTNNNNNAGG 1 cut(s) 1193
XapI RAATTY 5 cut(s) 908, 1170, 1465, 1676, 1771
XbaI TCTAGA 2 cut(s) 874, 1208
XceI RCATGY 5 cut(s) 474, 478, 956, 1319, 1500
XcmI CCANNNNNNNNNTGG 1 cut(s) 1656
XmnI GAANNNNTTC 1 cut(s) 1170
XspI CTAG 4 cut(s) 875, 1209, 1656, 2438
ZrmI AGTACT 1 cut(s) 347
Zsp2I ATGCAT 2 cut(s) 93, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.