RchiOBHm_Chr1g0320411

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
8047402 .. 8052712
5311 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55056

Sequence Viewer

Length: 4092 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGAGAGGTTAACGCCTCGGGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGCTGGCAAAAAGCTGCAGAAATGGAGAGACACACTGTCCGCAGTTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAGCAAAGGAGAGGGAGTGAAGCTGTGGCTGAATGACCTCAAACATTTGGCTTATGATATGGACGATCTACTCAACACCTTTTCTAATGAGATGTTAGAACGCGAGCAGCTCAAGGTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTCCGAAATAGACGAGATTGCTAATCGCTTGCAAGACATATTTGACCGGAAAGAGAAACTCGGTTTGAAATATATTGAGCAAACCTCTACGTCAACATCGTCATCAAAAAGGACACCAAGTTCGCCTGTGTTGGATGGATCTGTGGTTGGGAGAGATGAAGACGCAGGAAAGATTGTGGAATTGTTGTCCAGAAATGCTGATCCTTCTTCTCCCACAAATTATCAAGTTGTTGCCATTGTTGGTATGGGTGGACTCGGCAAGACAACGCTTGCGGGACGAGTCTTCAATGATGTAGTTGCAATGAAACAGTTTAGTCTCAAGATCTGGGTTTCAGTATCCGATAACTTTGATCTTCAAACGGTGACGAGAGCTATTTTTAAGGAAGTTACATCTCGGCCTTGTGATATGGATGAGTTCAGTCGACTTCAACATAATCTGAGCAAGGAGATCGATGGTAAAAAGTTTTTGATTGTTTTAGATGATGTCTGGAGCACATGTGATTATGATTTATGGAGAAGCCTGCAAGCTCCCCTCCTTGGCGGAGCCAAGGGAAGTAAGATAATGGTGACAACACGTGACGAAAAAGTTGCAATATTGATGGGAGCCCCAGCCGGTGATGTTTATCATTTAAAGACTCTATCAGAAGAAGATTGTTTGCAAGTATTTGAGCAGCATGTTAGCAATGACAGGCCACCGAATTTTGAGTTGCTTAAGGAGAAAATTGTTACGAATTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTGAAGAAACTGGCAAGTGGGAGGAAATACTGAATGACAAATTATGGAGTATGTCAGACAGGAGTAAGATACTCCCAGTACTCAGATTGAGTTATCATTATCTCCCTTCACATTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTAATCCTTCTGTGGATGGCAGAGGGTTTCCTTGAGCAAAGTCAGCAAACAGAAGGGACTAAAGGAATGGAAGATATCGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTATTTCAAACCTCGGGCAAAAACAGTTCATGCTATGTAATGCATGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGCAGACACATTTTGCAGATTGGAGGATAAACAGCATGGTAGATGTTCTGCAAAGACTCGTCATCTGAGTTACATTTCTGCTAAGTTTGATGGGGAAAAAAGATTTGAGACACTTTCTGGAGCAAAACGCTTGCGGACTTTCCTACCACTTCCTGTTTCCGGTGGTTATGAGAATTATCTAACTCGTTATGCTACTTTTGATTTATTGCCACGATTGAAGTACTTGCGGGTGCTCTCCTTTAATGGCTATAAACTGACCGAGCTGCCAGATTCTGTAGGTAAGTTGAAACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGAGTTTGCCTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAACTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATGCCTTCATTGGAAGAAATGCCTCCCAAAGTAAGTCGGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGATTGGAGAGATACAGTCATTGCATCTTCGTGGGACCTTGAGTGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGGAAATCTTGCAATTAGAATGGAGCGGCACAAGCGAGAAGGAGTTGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTAAGGAGCTGACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGAAGTCTGTGAAATTGGAGAATTGTAAAAACTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTCCTGAAAAATCTTCAGATACAAGGACTGGTCAATGTTGAAAGTGTGGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGTTGGAAGACCTGACGTTCCAGGACATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCGAGTTTTCCCATGCATGGAAAAGCTTTCCATCCTTGGTTGCCCCAAATTGAAAGATTGGTTGCCCAAGAGCATGGATTCATTATCAGAGCTGTATATTGCTGCATGTGAAGAGTTAGTGGTTTCAATTGCCAATTATAAACATCTTCGTGGGCTGTTCATCTGCGGTTGTAAGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGCTATTGGAGCATATGTGGCTTGCAAGTATTTCAGAGTTCAGACTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAAATTTGCGGATTACTGGTTGTGAAGAGTTGGCATGTTTATGGGAGAATGAGGATAGATGGCTGCAGCCTCGAATTTCTAATATTATTGGAGGCAACATATCTGACTCCCCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAAGGAACTTGTGCTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGTTGGTTGGCCACCTTGTCTTGAAAATGTAAAGATGGAGTCGTGTAATTCTTTGACATATTTCGTAAGGTATCAAGTACCGCCAAGCATAAGAAGAATAGAGATAGCAAAATGTCAAAATTTGAAACTACTGGTGAAGGATGCTTGGGAGCTAGAGGGTTGTCTGGAGTGGTTGGAGATAAAAGAGTGTGCATCTTTGACATCCTTATCAGGCAAAGGAGGCCGACTACCCAGAACCCTCAAATTTGTTAGAATAAGTGACTGTGAACGATTGGAGTCAATAATCAAAACATTCCACGAGGGCACTTGTCTTGATGCTCTTACGATAGAGAGGTGTGCAAATCTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCCGCTCTTCGCATGTTATGGGTAGAGGAATGTGGAAGTCTCGTTTCCTTCCCAAGCGGAGGGTTGCCATCCAGCCTGATATGCTTGTCTATCTCCATTTGTGCTCAATTGGAAGCCCTCCCCAGAGGCTATGTGGAAAACCTCAGCTCTCTTCAGATTTTGTTGTTGAGATGCTGGGGAGGTTTGGCATCCATTCTAGAAGAGGGTTTCCCACCCAACCTAATTGATCTGGAAATTGGCCCTCTCTCAGAGTGCGGGCTTCACCACTTGGGCAGACTCACCTCTCTTGAAACCTTTTCTATCTACTGTGTAGATCCAGATGTGGTGTCCTTTCCGCCAAAGGATGTGCTGCTCCCTAAATCTCTCATTAAACTCACCATTGCAGGCTTTCCAAATCTGAAGCGCCTGTCATCATCTTTTCAATCACTCACCTCTCTTGAATCCCTTGCCATTCTTGGATGCCCAAATCTAGCATCTATTATTCCAGAACAAGGGGATCATCTGCCTCCTTCACTTACACAGCTTCGCATCCGTGAGTTCTGTCCGCTGCTCACAAACAAATACCAACCAGGTAAAGGACGACACTGGCCCAAAGAAATTGCCCACATCCCTTACGTTTATGTTGGAGGCTGCGATGACGAAGCCGAAGCCGACCGCCGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1363

Amino Acids

153.12

Weight (kDa)

6.3

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 97 5e-17 Rx N-terminal domain
NB-ARC PF00931 181 - 354 1.3e-35 NB-ARC domain
WHD_DRP PF23559 434 - 505 5.1e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 578 - 844 7.7e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 694 - 813 1e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2712
Acc65I GGTACC 1 cut(s) 1824
AccB1I GGYRCC 1 cut(s) 1824
AccB7I CCANNNNNTGG 1 cut(s) 3053
AccBSI CCGCTC 2 cut(s) 2217, 3431
AccI GTMKAC 1 cut(s) 799
AccII CGCG 1 cut(s) 285
AclWI GGATC 5 cut(s) 64, 523, 572, 3734, 3930
AcoI YGGCCR 2 cut(s) 2401, 3059
AcsI RAATTY 8 cut(s) 1075, 1319, 2309, 2571, 2863, 2934, 3169, 3293
AcuI CTGAAG 4 cut(s) 20, 2411, 3563, 3845
AcvI CACGTG 1 cut(s) 953
AfaI GTAC 5 cut(s) 324, 1239, 1748, 1826, 3129
AfiI CCNNNNNNNGG 9 cut(s) 914, 1685, 2399, 2590, 3053, 3485, 3917, 4001, 4083
AflII CTTAAG 2 cut(s) 1088, 2273
AflIII ACRYGT 2 cut(s) 872, 950
AhdI GACNNNNNGTC 1 cut(s) 133
AjnI CCWGG 3 cut(s) 72, 2520, 3994
AjuI GAANNNNNNNTTGG 2 cut(s) 1326, 1358
AleI CACNNNNGTG 1 cut(s) 2760
AloI GAACNNNNNNTCC 2 cut(s) 481, 513
Alw21I GWGCWC 5 cut(s) 872, 1761, 2757, 3001, 3532
Alw26I GTCTC 5 cut(s) 120, 698, 1446, 1628, 3470
Alw44I GTGCAC 1 cut(s) 2753
AlwI GGATC 5 cut(s) 64, 523, 572, 3734, 3930
AlwNI CAGNNNCTG 2 cut(s) 43, 1799
Ama87I CYCGRG 2 cut(s) 63, 1462
AoxI GGCC 7 cut(s) 773, 1067, 2401, 3059, 3271, 3664, 4013
ApaLI GTGCAC 1 cut(s) 2753
ApoI RAATTY 8 cut(s) 1075, 1319, 2309, 2571, 2863, 2934, 3169, 3293
ArsI GACNNNNNNTTYG 6 cut(s) 481, 513, 1304, 1336, 1409, 1441
AseI ATTAAT 2 cut(s) 1337, 1847
Asp700I GAANNNNTTC 1 cut(s) 2571
Asp718I GGTACC 1 cut(s) 1824
AspLEI GCGC 1 cut(s) 3831
AspS9I GGNCC 3 cut(s) 2112, 3665, 4014
AsuC2I CCSGG 1 cut(s) 4085
AsuII TTCGAA 1 cut(s) 2307
AvaI CYCGRG 2 cut(s) 63, 1462
AvaII GGWCC 1 cut(s) 2112
BaeGI GKGCMC 2 cut(s) 2757, 3356
BalI TGGCCA 2 cut(s) 2403, 3061
BanI GGYRCC 1 cut(s) 1824
BanII GRGCYC 1 cut(s) 985
BauI CACGAG 1 cut(s) 3347
BbrPI CACGTG 1 cut(s) 953
BbsI GAAGAC 5 cut(s) 543, 652, 1931, 2168, 2514
Bbv12I GWGCWC 5 cut(s) 872, 1761, 2757, 3001, 3532
BbvCI CCTCAGC 1 cut(s) 3569
BceAI ACGGC 1 cut(s) 2181
BcgI CGANNNNNNTGC 4 cut(s) 808, 842, 947, 981
BciT130I CCWGG 3 cut(s) 74, 2522, 3996
BciVI GTATCC 1 cut(s) 724
BcnI CCSGG 1 cut(s) 4085
BcoDI GTCTC 5 cut(s) 120, 698, 1446, 1628, 3470
BfaI CTAG 3 cut(s) 3203, 3623, 3896
BfmI CTRYAG 4 cut(s) 113, 1527, 1800, 2924
BfoI RGCGCY 1 cut(s) 3832
BfrI CTTAAG 2 cut(s) 1088, 2273
BfuI GTATCC 1 cut(s) 724
BglII AGATCT 2 cut(s) 15, 699
BmcAI AGTACT 2 cut(s) 1239, 1748
Bme1390I CCNGG 4 cut(s) 74, 2522, 3996, 4085
Bme18I GGWCC 1 cut(s) 2112
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 63, 1462
BmgT120I GGNCC 3 cut(s) 2112, 3665, 4014
BmiI GGNNCC 4 cut(s) 922, 982, 1826, 2113
BmrFI CCNGG 4 cut(s) 74, 2522, 3996, 4085
BmrI ACTGGG 1 cut(s) 1229
BmuI ACTGGG 1 cut(s) 1229
BpiI GAAGAC 5 cut(s) 543, 652, 1931, 2168, 2514
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 4 cut(s) 68, 886, 1665, 3236
Bpu10I CCTNAGC 1 cut(s) 3569
Bpu14I TTCGAA 1 cut(s) 2307
BpuEI CTTGAG 5 cut(s) 278, 680, 1388, 2137, 2987
BpuMI CCSGG 1 cut(s) 4085
Bsa29I ATCGAT 1 cut(s) 828
BsaAI YACGTR 1 cut(s) 953
BsaBI GATNNNNATC 1 cut(s) 999
BsaJI CCNNGG 6 cut(s) 62, 73, 913, 924, 1461, 2608
BsaWI WCCGGW 2 cut(s) 423, 1685
Bsc4I CCNNNNNNNGG 9 cut(s) 914, 1685, 2399, 2590, 3053, 3485, 3917, 4001, 4083
Bse118I RCCGGY 1 cut(s) 989
Bse1I ACTGG 9 cut(s) 313, 1174, 1235, 2445, 2498, 2881, 3025, 3186, 4016
Bse3DI GCAATG 5 cut(s) 684, 1066, 1120, 2096, 3804
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 3 cut(s) 74, 2522, 3996
BseCI ATCGAT 1 cut(s) 828
BseDI CCNNGG 6 cut(s) 62, 73, 913, 924, 1461, 2608
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 9 cut(s) 914, 1685, 2399, 2590, 3053, 3485, 3917, 4001, 4083
BseMI GCAATG 5 cut(s) 684, 1066, 1120, 2096, 3804
BseMII CTCAG 7 cut(s) 806, 1255, 1583, 1854, 2457, 3583, 3687
BseNI ACTGG 9 cut(s) 313, 1174, 1235, 2445, 2498, 2881, 3025, 3186, 4016
BseRI GAGGAG 3 cut(s) 111, 182, 2385
BseSI GKGCMC 2 cut(s) 2757, 3356
BseYI CCCAGC 2 cut(s) 985, 3600
BsgI GTGCAG 2 cut(s) 2269, 2503
Bsh1236I CGCG 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 4081
BshFI GGCC 7 cut(s) 775, 1069, 2403, 3061, 3273, 3666, 4015
BshNI GGYRCC 1 cut(s) 1824
BshVI ATCGAT 1 cut(s) 828
BsiEI CGRYCG 1 cut(s) 4081
BsiHKAI GWGCWC 5 cut(s) 872, 1761, 2757, 3001, 3532
BsiHKCI CYCGRG 2 cut(s) 63, 1462
BsiSI CCGG 4 cut(s) 424, 990, 1686, 4084
BslFI GGGAC 3 cut(s) 666, 1405, 2125
BslI CCNNNNNNNGG 9 cut(s) 914, 1685, 2399, 2590, 3053, 3485, 3917, 4001, 4083
BsmAI GTCTC 5 cut(s) 120, 698, 1446, 1628, 3470
BsmFI GGGAC 3 cut(s) 666, 1405, 2125
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 7 cut(s) 775, 1069, 2403, 3061, 3273, 3666, 4015
BsoBI CYCGRG 2 cut(s) 63, 1462
Bsp119I TTCGAA 1 cut(s) 2307
Bsp1286I GDGCHC 7 cut(s) 872, 985, 1761, 2757, 3001, 3356, 3532
BspANI GGCC 7 cut(s) 775, 1069, 2403, 3061, 3273, 3666, 4015
BspCNI CTCAG 7 cut(s) 807, 1254, 1584, 1855, 2458, 3582, 3686
BspDI ATCGAT 1 cut(s) 828
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 4 cut(s) 922, 982, 1826, 2113
BspMAI CTGCAG 3 cut(s) 117, 1531, 2928
BspPI GGATC 5 cut(s) 64, 523, 572, 3734, 3930
BspQI GCTCTTC 1 cut(s) 3438
BspT104I TTCGAA 1 cut(s) 2307
BspT107I GGYRCC 1 cut(s) 1824
BspTI CTTAAG 2 cut(s) 1088, 2273
BsrBI CCGCTC 2 cut(s) 2217, 3431
BsrDI GCAATG 5 cut(s) 684, 1066, 1120, 2096, 3804
BsrFI RCCGGY 1 cut(s) 989
BsrI ACTGG 9 cut(s) 313, 1174, 1235, 2445, 2498, 2881, 3025, 3186, 4016
BssAI RCCGGY 1 cut(s) 989
BssECI CCNNGG 6 cut(s) 62, 73, 913, 924, 1461, 2608
BssSI CACGAG 1 cut(s) 3347
BssT1I CCWWGG 3 cut(s) 913, 924, 2608
Bst2BI CACGAG 1 cut(s) 3347
Bst2UI CCWGG 3 cut(s) 74, 2522, 3996
Bst6I CTCTTC 6 cut(s) 1271, 2679, 2880, 3438, 3582, 3621
BstAFI CTTAAG 2 cut(s) 1088, 2273
BstBAI YACGTR 1 cut(s) 953
BstBI TTCGAA 1 cut(s) 2307
BstDEI CTNAG 9 cut(s) 815, 1241, 1592, 1608, 1863, 2466, 2993, 3569, 3673
BstFNI CGCG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 3832
BstHHI GCGC 1 cut(s) 3831
BstMAI GTCTC 5 cut(s) 120, 698, 1446, 1628, 3470
BstMCI CGRYCG 1 cut(s) 4081
BstNI CCWGG 3 cut(s) 74, 2522, 3996
BstNSI RCATGY 9 cut(s) 876, 1055, 1875, 1879, 1987, 2530, 2682, 2898, 3442
BstSCI CCNGG 4 cut(s) 72, 2520, 3994, 4083
BstSFI CTRYAG 4 cut(s) 113, 1527, 1800, 2924
BstSLI GKGCMC 2 cut(s) 2757, 3356
BstUI CGCG 1 cut(s) 285
BstV2I GAAGAC 5 cut(s) 543, 652, 1931, 2168, 2514
BstX2I RGATCY 5 cut(s) 15, 69, 515, 699, 3739
BstXI CCANNNNNNTGG 1 cut(s) 2647
BstYI RGATCY 5 cut(s) 15, 69, 515, 699, 3739
Bsu15I ATCGAT 1 cut(s) 828
BsuI GTATCC 1 cut(s) 724
BsuRI GGCC 7 cut(s) 775, 1069, 2403, 3061, 3273, 3666, 4015
BsuTUI ATCGAT 1 cut(s) 828
BtgZI GCGATG 1 cut(s) 4074
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 4 cut(s) 12, 131, 2505, 4009
CaiI CAGNNNCTG 2 cut(s) 43, 1799
CfoI GCGC 1 cut(s) 3831
Cfr10I RCCGGY 1 cut(s) 989
Cfr13I GGNCC 3 cut(s) 2112, 3665, 4014
ClaI ATCGAT 1 cut(s) 828
CseI GACGC 1 cut(s) 548
CsiI ACCWGGT 1 cut(s) 3994
Csp6I GTAC 5 cut(s) 323, 1238, 1747, 1825, 3128
CviQI GTAC 5 cut(s) 323, 1238, 1747, 1825, 3128
DdeI CTNAG 9 cut(s) 815, 1241, 1592, 1608, 1863, 2466, 2993, 3569, 3673
DraI TTTAAA 1 cut(s) 1008
DriI GACNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 2 cut(s) 2401, 3059
Eam1104I CTCTTC 6 cut(s) 1271, 2679, 2880, 3438, 3582, 3621
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 6 cut(s) 1271, 2679, 2880, 3438, 3582, 3621
EciI GGCGGA 2 cut(s) 933, 3750
Eco130I CCWWGG 3 cut(s) 913, 924, 2608
Eco24I GRGCYC 1 cut(s) 985
Eco32I GATATC 1 cut(s) 1411
Eco47I GGWCC 1 cut(s) 2112
Eco57I CTGAAG 4 cut(s) 20, 2411, 3563, 3845
Eco72I CACGTG 1 cut(s) 953
Eco88I CYCGRG 2 cut(s) 63, 1462
EcoO109I RGGNCCY 1 cut(s) 2112
EcoRII CCWGG 3 cut(s) 72, 2520, 3994
EcoRV GATATC 1 cut(s) 1411
EcoT14I CCWWGG 3 cut(s) 913, 924, 2608
EcoT22I ATGCAT 3 cut(s) 1494, 1877, 2591
EcoT38I GRGCYC 1 cut(s) 985
ErhI CCWWGG 3 cut(s) 913, 924, 2608
FalI AAGNNNNNCTT 2 cut(s) 1268, 1300
FaqI GGGAC 3 cut(s) 666, 1405, 2125
FauI CCCGC 3 cut(s) 643, 1746, 3674
FauNDI CATATG 1 cut(s) 2790
FblI GTMKAC 1 cut(s) 799
FriOI GRGCYC 1 cut(s) 985
FspBI CTAG 3 cut(s) 3203, 3623, 3896
GlaI GCGC 1 cut(s) 3830
GsaI CCCAGC 2 cut(s) 989, 3604
GsuI CTGGAG 4 cut(s) 68, 886, 1665, 3236
HaeII RGCGCY 1 cut(s) 3832
HaeIII GGCC 7 cut(s) 775, 1069, 2403, 3061, 3273, 3666, 4015
HapII CCGG 4 cut(s) 424, 990, 1686, 4084
HgaI GACGC 1 cut(s) 548
HhaI GCGC 1 cut(s) 3831
Hin6I GCGC 1 cut(s) 3829
HinP1I GCGC 1 cut(s) 3829
HincII GTYRAC 5 cut(s) 57, 471, 800, 1869, 2025
HindII GTYRAC 5 cut(s) 57, 471, 800, 1869, 2025
HindIII AAGCTT 4 cut(s) 88, 2270, 2597, 2855
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 4 cut(s) 424, 990, 1686, 4084
Hpy166II GTNNAC 9 cut(s) 57, 301, 471, 629, 800, 1869, 2025, 2755, 3317
Hpy8I GTNNAC 9 cut(s) 57, 301, 471, 629, 800, 1869, 2025, 2755, 3317
HpyCH4IV ACGT 7 cut(s) 467, 952, 1156, 1895, 1963, 2516, 4041
HpyF3I CTNAG 9 cut(s) 815, 1241, 1592, 1608, 1863, 2466, 2993, 3569, 3673
HpySE526I ACGT 7 cut(s) 467, 952, 1156, 1895, 1963, 2516, 4041
HspAI GCGC 1 cut(s) 3829
KpnI GGTACC 1 cut(s) 1828
KspAI GTTAAC 1 cut(s) 57
LguI GCTCTTC 1 cut(s) 3438
MabI ACCWGGT 1 cut(s) 3994
MaeI CTAG 3 cut(s) 3203, 3623, 3896
MaeII ACGT 7 cut(s) 467, 952, 1156, 1895, 1963, 2516, 4041
MaeIII GTNAC 8 cut(s) 739, 763, 943, 953, 1102, 1415, 1595, 3308
MbiI CCGCTC 2 cut(s) 2217, 3431
MfeI CAATTG 2 cut(s) 2700, 3533
MflI RGATCY 5 cut(s) 15, 69, 515, 699, 3739
MhlI GDGCHC 7 cut(s) 872, 985, 1761, 2757, 3001, 3356, 3532
MlsI TGGCCA 2 cut(s) 2403, 3061
MluNI TGGCCA 2 cut(s) 2403, 3061
MmeI TCCRAC 6 cut(s) 489, 2104, 2217, 2484, 3204, 4030
Mox20I TGGCCA 2 cut(s) 2403, 3061
Mph1103I ATGCAT 3 cut(s) 1494, 1877, 2591
MroXI GAANNNNTTC 1 cut(s) 2571
MscI TGGCCA 2 cut(s) 2403, 3061
MslI CAYNNNNRTG 4 cut(s) 2106, 2721, 2760, 2899
Msp20I TGGCCA 2 cut(s) 2403, 3061
MspA1I CMGCKG 3 cut(s) 149, 178, 3973
MspCI CTTAAG 2 cut(s) 1088, 2273
MspI CCGG 4 cut(s) 424, 990, 1686, 4084
MspR9I CCNGG 4 cut(s) 74, 2522, 3996, 4085
MunI CAATTG 2 cut(s) 2700, 3533
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 3 cut(s) 74, 2522, 3996
MvnI CGCG 1 cut(s) 285
NciI CCSGG 1 cut(s) 4085
NdeI CATATG 1 cut(s) 2790
NlaIV GGNNCC 4 cut(s) 922, 982, 1826, 2113
NmeAIII GCCGAG 2 cut(s) 612, 751
NmuCI GTSAC 5 cut(s) 739, 943, 953, 1415, 3308
NsiI ATGCAT 3 cut(s) 1494, 1877, 2591
NspI RCATGY 9 cut(s) 876, 1055, 1875, 1879, 1987, 2530, 2682, 2898, 3442
NspV TTCGAA 1 cut(s) 2307
OliI CACNNNNGTG 1 cut(s) 2760
PaeI GCATGC 1 cut(s) 1879
PciI ACATGT 1 cut(s) 872
PciSI GCTCTTC 1 cut(s) 3438
PcsI WCGNNNNNNNCGW 1 cut(s) 473
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 2571
PfeI GAWTC 4 cut(s) 1796, 2385, 2651, 3866
PflMI CCANNNNNTGG 1 cut(s) 3053
PfoI TCCNGGA 1 cut(s) 2520
PmaCI CACGTG 1 cut(s) 953
PmlI CACGTG 1 cut(s) 953
Ppu21I YACGTR 1 cut(s) 953
PpuMI RGGWCCY 1 cut(s) 2112
PscI ACATGT 1 cut(s) 872
PshBI ATTAAT 2 cut(s) 1337, 1847
PsiI TTATAA 1 cut(s) 2712
Psp5II RGGWCCY 1 cut(s) 2112
Psp6I CCWGG 3 cut(s) 72, 2520, 3994
PspCI CACGTG 1 cut(s) 953
PspFI CCCAGC 2 cut(s) 985, 3600
PspGI CCWGG 3 cut(s) 72, 2520, 3994
PspN4I GGNNCC 4 cut(s) 922, 982, 1826, 2113
PspPI GGNCC 3 cut(s) 2112, 3665, 4014
PspPPI RGGWCCY 1 cut(s) 2112
PstI CTGCAG 3 cut(s) 117, 1531, 2928
PstNI CAGNNNCTG 2 cut(s) 43, 1799
PsuI RGATCY 5 cut(s) 15, 69, 515, 699, 3739
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 5 cut(s) 324, 1239, 1748, 1826, 3129
RsaNI GTAC 5 cut(s) 323, 1238, 1747, 1825, 3128
RseI CAYNNNNRTG 4 cut(s) 2106, 2721, 2760, 2899
SalI GTCGAC 1 cut(s) 798
SapI GCTCTTC 1 cut(s) 3438
Sau96I GGNCC 3 cut(s) 2112, 3665, 4014
ScaI AGTACT 2 cut(s) 1239, 1748
ScrFI CCNGG 4 cut(s) 74, 2522, 3996, 4085
SduI GDGCHC 7 cut(s) 872, 985, 1761, 2757, 3001, 3356, 3532
SexAI ACCWGGT 1 cut(s) 3994
SfcI CTRYAG 4 cut(s) 113, 1527, 1800, 2924
SfuI TTCGAA 1 cut(s) 2307
SinI GGWCC 1 cut(s) 2112
SmiMI CAYNNNNRTG 4 cut(s) 2106, 2721, 2760, 2899
SmlI CTYRAG 7 cut(s) 293, 695, 1088, 1367, 2116, 2273, 3002
SmoI CTYRAG 7 cut(s) 293, 695, 1088, 1367, 2116, 2273, 3002
SphI GCATGC 1 cut(s) 1879
SspI AATATT 4 cut(s) 972, 1424, 1902, 2944
SspMI CTAG 3 cut(s) 3203, 3623, 3896
StyD4I CCNGG 4 cut(s) 72, 2520, 3994, 4083
StyI CCWWGG 3 cut(s) 913, 924, 2608
TaiI ACGT 7 cut(s) 470, 955, 1159, 1898, 1966, 2519, 4044
TaqI TCGA 7 cut(s) 799, 828, 2307, 2383, 2575, 2823, 2932
TaqII GACCGA 2 cut(s) 1434, 1799
TatI WGTACW 2 cut(s) 1237, 1746
TauI GCSGC 2 cut(s) 29, 2220
TfiI GAWTC 4 cut(s) 1796, 2385, 2651, 3866
TscAI CASTG 4 cut(s) 12, 138, 2505, 4016
TseFI GTSAC 5 cut(s) 739, 943, 953, 1415, 3308
Tsp45I GTSAC 5 cut(s) 739, 943, 953, 1415, 3308
TspGWI ACGGA 1 cut(s) 3947
TspRI CASTG 4 cut(s) 12, 138, 2505, 4016
Van91I CCANNNNNTGG 1 cut(s) 3053
Vha464I CTTAAG 2 cut(s) 1088, 2273
VneI GTGCAC 1 cut(s) 2753
VpaK11BI GGWCC 1 cut(s) 2112
VspI ATTAAT 2 cut(s) 1337, 1847
XapI RAATTY 8 cut(s) 1075, 1319, 2309, 2571, 2863, 2934, 3169, 3293
XbaI TCTAGA 1 cut(s) 3622
XceI RCATGY 9 cut(s) 876, 1055, 1875, 1879, 1987, 2530, 2682, 2898, 3442
XcmI CCANNNNNNNNNTGG 3 cut(s) 619, 3054, 3556
XmiI GTMKAC 1 cut(s) 799
XmnI GAANNNNTTC 1 cut(s) 2571
XspI CTAG 3 cut(s) 3203, 3623, 3896
ZrmI AGTACT 2 cut(s) 1239, 1748
Zsp2I ATGCAT 3 cut(s) 1494, 1877, 2591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.