Rmu_sc0000861.1_g000024

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000861.1
OG0000070
Physical Location & Seq
Reverse (-)
102571 .. 106737
4167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000861.1_g000024.1.cds

Sequence Viewer

Length: 4167 bp
atgctttcagaaaacaactctgtactagttttgattgctttgcttgttcctctgtcatcttctttgtcactctcacaaatcacaactttgattgcttccttcctgaaactaactatggcagtgggagaggtctttcttgctgcgttccttcaggtgctattcgacaggttagcctctcgtgagttcctcaaatttgcacggcagaagggcattggcgggaagcttgaaaaatggagtgaaacactgtctgcaattcgagcagtgttgtatgacgccgaggagaagcaactcataagcatgtcagtgaaagagtggctggatgatctcaaagacttggcttatgatgtggaggacatcttggataggttttccactgacatgttacgattcaaggccaaggagcaaaatctggctggcacaagcaaggtacgaggcctctttcttaaagttaaatgcaacttcaatatgaactctgaaatggaaaagatttctgaacgattgcaagaaatatctcaacgaaaaaacatgctcggtttgaataatagcggagctggatctagtgcggcatcccaaaggcgaacaagctcatgcttgcttgatggacctgtggttggaaggtatgaagacaaaaggaagattattgctttgttgtcaagagatgaacctagtactgtcaatttccatgtagttgcaattgttggcatgcctggagttgggaagactacacttgctaaatcagtattcaaggacaacaatattgccctggaacagttcgatcttaaggtatgggtatcagtgtctgatgattttgaccttgttagagtaacaaaagccattcttgaatcggtcacgtctgtgtattgtgacttcaaggaattcaataaagttcaagataacttgagcaaggagttagcgagtaaaaagtttctaattgttttggatgatgtttggaatacctgtgaacatgatcaatggacaaccctacagtctccctttcatgtcggagcaccaggaagtaagataattgtaacgactcgtgatgaaaaagttgcaagaatgacgagacccattgagatccactgtttgaagtatatgtcagatgatgattgttgggaagtctttcagcagcatgcattcatgaatgtcaacagtgggagaccacaaggttttgaattttttcgagagagtattgttagaaaatgtggcggattgcccttggctgcaaggactctcggtggtcttttaggttgtgaagacatagatgagtggaaaaaaatattgaacaatagattatggagtctatcaaataagagtcacattcaccaagtgttgaaattgagctattactatcttccttcatatttgaaaaggtgctttgcatattgctcaatactcccaaacgactatgaatttggggagaagcaattgacacttctgtggatggcagagggtttacttcaacaagaagaagaacatgaaacattagaagacttgggtggtcactattttggacagctattatctaggttattatttcaaaaatcaagcagaaacagttcattatatgttatgcatcaccttgttggtgatctggctcggtgggctgcgggagaaatttgtctcaggttggaggataaagatggtagatgttctcgaaagacacgtcattcgtcatacattcccggtcagtatgatggggttaacaaatttgaggcattctctgaagccagacgtttgcgtacttttctaccattgtcagaagacttttgtaaccatgtgacttgtaaggttactcttgatttactgccaagattggaatacttacgggtactctctttgaatggctatcaaataactgagttgccgaattcaattggtaacttgaagcatctacggtaccttgatgtttctcatacattaataatgagattgcctgaatcaacaagcaccctttgcaacttacagacattaatattagaaagttgttctagactgaagacactgcctacaaagatgcgatatttatcaaatctacgccatctcaacaatttaaatgtgccttcattggaaggaatgcccccacagctaagtcaactgagcaatctgcaaacgttgccgaatttcgtggtgggcaaaggtagtgactcaagggtaagagagatagggcccctgtctcttcttcgagggacattgtgcctctcaagattggaaaatgtcaatgatgttgaggatgcaaggaggagtgacttaatttgcaaggaagggattgatgcattgcagctggaatggaatgataaaggagaaaatgaatcgatcgtgcttgacatgttaaaacctcacaaaaagatgaaagaggtcatcatcaagggttatggaggtttgcagttttcagcatggattggagatcctttattctcttacgtggtgcatgtgaggttagagaattgtaacaattgtcgattcttgccaccacttggacaattgccttctctccaaatactttgtattagaaatatgtctggggtggaaaaagttggcctagagttttgtggagagggtagcttgtcttttccattattggagacccttgtgtttgaggatatgcaaaactggaatgagtggtttccttgccaaggatatcaaggactagaagttttcccttccttgaaaatactttcagtgaaaagatgtcccaaattaaagggtgagttacctcacaagcttgatttgttaacaaagcttgagattcatgaatgtaatgaactggtggtttcaattggcaactacagacagctctgtgaattaaacgtgcgcgattgcaaaggggtggtgtataaaagtgtgcaccactttgagttactcaagtctatttatctttcagatttttcagtgctgagatttcaaactggaggattcatgaccggattaagaaaggtggaacaattgatgatagccaatggtgatgaactttcatctgaatggcagaatgaaggtagattgctgcaacacctgatatcttttcgaagtttgttcattgaaagaaactcccaccttcttcaattgcattgcctgacttcacttcaaatgcttcacatatatagatgcccaagtctagtttccattccagagtcatgtttgccagcttctcttaaagatatagagattgaaaggtgcggttctctgacgtgctttgcaagatacaaaatacccccgagtctaagaacaatagagattaaacattgccagtgtttgaaatatttggtagaggaggagcaggtggagggttgctcctctgcttcatcttctcttgagtacttgagcatatctaactgtccatctcttatctccttatcatccagaggtggattaccaaaggcacttaaacacctttacataagggattgtgaacaactggagacaatatctgaaggcttctatgatgacacttgcctcgaagaacttcatattgggcgttgtgcaaatctgaaattcttaccggagggcctatgtcttcttgccaatcttcgtaggttaattgtttttgtctgtggaagtcttgtttccttcccaagaggagggttgccgacaaggccctccaacctgacattccttgttatctctgattgtcaaagatggaaagccttccccaaaggcatgatcaacctcaactctctccaggagctggtgattggaagctgtgaaggtttggcgtccattctagaggagggttttcccccgaacctaacatcacttatccttttgaatcccagcaattgtaagccactcacagactggggcttgcacttgcacagactcacctctcttacagagttatgcatacaaggtgttgatcaagacttggtttcctttccaccagaggaaatgctgcttcctacatctctcattaaacttaccattggattcttcccgaatctgagacgaatatccagccagggccttcaagtcctcacctctcttgaatctctcagaattgatgattgcccaaagcttgcatccattccagaggacctgccctcttcactcacacaactttacatctatgatgactgtcctctgcttagaaagagatataaaccagatataccacgatactgggccaaaataagtcacattccgtacatagagattggagactacaaaccctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1388

Amino Acids

157.41

Weight (kDa)

6.64

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3265
Acc36I ACCTGC 2 cut(s) 3265, 4038
Acc65I GGTACC 1 cut(s) 1896
AccB1I GGYRCC 1 cut(s) 1896
AccB7I CCANNNNNTGG 2 cut(s) 2474, 3682
AccII CGCG 1 cut(s) 2814
AciI CCGC 6 cut(s) 216, 546, 563, 1206, 1607, 3174
AclI AACGTT 1 cut(s) 2111
AclWI GGATC 3 cut(s) 562, 1069, 2399
AcsI RAATTY 9 cut(s) 191, 875, 1172, 1409, 1614, 1706, 1867, 2119, 3488
AcuI CTGAAG 4 cut(s) 134, 1743, 2015, 3447
AcyI GRCGYC 2 cut(s) 273, 3710
AdeI CACNNNGTG 1 cut(s) 1327
AfaI GTAC 8 cut(s) 24, 429, 670, 1741, 1830, 1898, 3314, 4139
AfiI CCNNNNNNNGG 6 cut(s) 611, 713, 2474, 2633, 3575, 3682
AflII CTTAAG 1 cut(s) 779
AflIII ACRYGT 3 cut(s) 378, 1661, 2325
AhlI ACTAGT 1 cut(s) 25
AjiI CACGTC 3 cut(s) 852, 1664, 3186
AjnI CCWGG 5 cut(s) 706, 762, 1009, 3675, 3951
AjuI GAANNNNNNNTTGG 6 cut(s) 3100, 3132, 3450, 3482, 3590, 3622
AleI CACNNNNGTG 2 cut(s) 854, 1435
Alw21I GWGCWC 2 cut(s) 1009, 2847
Alw26I GTCTC 9 cut(s) 993, 1057, 1150, 1625, 2178, 2576, 3410, 3931, 4146
Alw44I GTGCAC 1 cut(s) 2843
AlwI GGATC 3 cut(s) 562, 1069, 2399
AlwNI CAGNNNCTG 2 cut(s) 800, 3682
Ama87I CYCGRG 1 cut(s) 3211
AoxI GGCC 8 cut(s) 393, 433, 2165, 2536, 3502, 3590, 3955, 4116
ApaI GGGCCC 1 cut(s) 2169
ApaLI GTGCAC 1 cut(s) 2843
ApeKI GCWGC 7 cut(s) 140, 1126, 1220, 1604, 2278, 3001, 3886
ApoI RAATTY 9 cut(s) 191, 875, 1172, 1409, 1614, 1706, 1867, 2119, 3488
ArsI GACNNNNNNTTYG 6 cut(s) 1394, 1426, 1648, 1651, 1680, 1683
AseI ATTAAT 2 cut(s) 1919, 1970
Asp700I GAANNNNTTC 5 cut(s) 1119, 1176, 1555, 3459, 3641
Asp718I GGTACC 1 cut(s) 1896
AspLEI GCGC 1 cut(s) 2814
AspS9I GGNCC 8 cut(s) 602, 2165, 2166, 3502, 3591, 3955, 4027, 4116
AsuC2I CCSGG 1 cut(s) 1683
AsuHPI GGTGA 8 cut(s) 1313, 1568, 1598, 2717, 2972, 3697, 3808, 3961
AsuII TTCGAA 1 cut(s) 3022
AvaI CYCGRG 1 cut(s) 3211
AvaII GGWCC 2 cut(s) 602, 4027
BaeGI GKGCMC 2 cut(s) 2169, 2847
BanI GGYRCC 1 cut(s) 1896
BanII GRGCYC 1 cut(s) 2169
BarI GAAGNNNNNNTAC 2 cut(s) 851, 883
BauI CACGAG 2 cut(s) 177, 1035
BbsI GAAGAC 7 cut(s) 630, 725, 1260, 1494, 1767, 2003, 3503
Bbv12I GWGCWC 2 cut(s) 1009, 2847
BbvI GCAGC 7 cut(s) 127, 1138, 1207, 1591, 2290, 2988, 3873
BccI CCATC 7 cut(s) 593, 1435, 1634, 1688, 2046, 3343, 3627
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 5 cut(s) 708, 764, 1011, 3677, 3953
BclI TGATCA 3 cut(s) 967, 3657, 3850
BcnI CCSGG 1 cut(s) 1683
BcoDI GTCTC 9 cut(s) 993, 1057, 1150, 1625, 2178, 2576, 3410, 3931, 4146
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 9 cut(s) 26, 558, 666, 1524, 1989, 2540, 2648, 3113, 3719
BfmI CTRYAG 2 cut(s) 983, 2785
BfrI CTTAAG 1 cut(s) 779
BfuAI ACCTGC 2 cut(s) 3265, 4038
BglI GCCNNNNNGGC 1 cut(s) 3589
BisI GCNGC 8 cut(s) 141, 564, 1127, 1221, 1605, 2279, 3002, 3887
BlsI GCNGC 8 cut(s) 142, 565, 1128, 1222, 1606, 2280, 3003, 3888
BmcAI AGTACT 2 cut(s) 670, 3314
Bme1390I CCNGG 6 cut(s) 708, 764, 1011, 1683, 3677, 3953
Bme18I GGWCC 2 cut(s) 602, 4027
BmeT110I CYCGRG 1 cut(s) 3211
BmgBI CACGTC 3 cut(s) 852, 1664, 3186
BmgT120I GGNCC 8 cut(s) 602, 2165, 2166, 3502, 3591, 3955, 4027, 4116
BmiI GGNNCC 3 cut(s) 1898, 2167, 2168
BmrFI CCNGG 6 cut(s) 708, 764, 1011, 1683, 3677, 3953
BmrI ACTGGG 2 cut(s) 3802, 4123
BmsI GCATC 8 cut(s) 575, 1582, 1897, 2004, 2221, 2260, 3092, 4022
BmuI ACTGGG 2 cut(s) 3802, 4123
BpiI GAAGAC 7 cut(s) 630, 725, 1260, 1494, 1767, 2003, 3503
BplI GAGNNNNNCTC 4 cut(s) 1703, 1735, 3272, 3304
BpmI CTGGAG 4 cut(s) 729, 2928, 3434, 3659
Bpu14I TTCGAA 1 cut(s) 3022
BpuEI CTTGAG 7 cut(s) 919, 2131, 2185, 2762, 2846, 3329, 3337
BpuMI CCSGG 1 cut(s) 1683
Bsa29I ATCGAT 1 cut(s) 2312
BsaAI YACGTR 1 cut(s) 2422
BsaBI GATNNNNATC 1 cut(s) 2023
BsaHI GRCGYC 2 cut(s) 273, 3710
BsaI GGTCTC 3 cut(s) 1057, 1150, 2576
BsaJI CCNNGG 6 cut(s) 276, 396, 762, 1215, 2632, 3952
BsaWI WCCGGW 2 cut(s) 2921, 3496
Bsc4I CCNNNNNNNGG 6 cut(s) 611, 713, 2474, 2633, 3575, 3682
Bse1I ACTGG 7 cut(s) 2615, 2769, 2911, 3244, 3417, 3797, 4118
Bse3DI GCAATG 3 cut(s) 2273, 3064, 3238
Bse8I GATNNNNATC 1 cut(s) 2023
BseBI CCWGG 5 cut(s) 708, 764, 1011, 3677, 3953
BseCI ATCGAT 1 cut(s) 2312
BseDI CCNNGG 6 cut(s) 276, 396, 762, 1215, 2632, 3952
BseGI GGATG 7 cut(s) 325, 566, 946, 1446, 2236, 3353, 4013
BseJI GATNNNNATC 1 cut(s) 2023
BseLI CCNNNNNNNGG 6 cut(s) 611, 713, 2474, 2633, 3575, 3682
BseMI GCAATG 3 cut(s) 2273, 3064, 3238
BseMII CTCAG 6 cut(s) 1636, 1848, 2087, 2885, 3926, 4000
BseNI ACTGG 7 cut(s) 2615, 2769, 2911, 3244, 3417, 3797, 4118
BseRI GAGGAG 7 cut(s) 293, 2254, 3280, 3281, 3284, 3588, 3737
BseSI GKGCMC 2 cut(s) 2169, 2847
BseXI GCAGC 7 cut(s) 127, 1138, 1207, 1591, 2290, 2988, 3873
BseYI CCCAGC 1 cut(s) 3767
Bsh1236I CGCG 1 cut(s) 2814
Bsh1285I CGRYCG 1 cut(s) 2316
BshFI GGCC 8 cut(s) 395, 435, 2167, 2538, 3504, 3592, 3957, 4118
BshNI GGYRCC 1 cut(s) 1896
BshVI ATCGAT 1 cut(s) 2312
BsiEI CGRYCG 1 cut(s) 2316
BsiHKAI GWGCWC 2 cut(s) 1009, 2847
BsiHKCI CYCGRG 1 cut(s) 3211
BsiSI CCGG 3 cut(s) 1683, 2922, 3497
BslFI GGGAC 2 cut(s) 2200, 2676
BslI CCNNNNNNNGG 6 cut(s) 611, 713, 2474, 2633, 3575, 3682
BsmAI GTCTC 9 cut(s) 993, 1057, 1150, 1625, 2178, 2576, 3410, 3931, 4146
BsmBI CGTCTC 1 cut(s) 3931
BsmFI GGGAC 2 cut(s) 2200, 2676
BsmI GAATGC 3 cut(s) 1133, 1715, 2079
BsnI GGCC 8 cut(s) 395, 435, 2167, 2538, 3504, 3592, 3957, 4118
Bso31I GGTCTC 3 cut(s) 1057, 1150, 2576
BsoBI CYCGRG 1 cut(s) 3211
Bsp119I TTCGAA 1 cut(s) 3022
Bsp120I GGGCCC 1 cut(s) 2165
Bsp1286I GDGCHC 3 cut(s) 1009, 2169, 2847
BspACI CCGC 6 cut(s) 216, 546, 563, 1206, 1607, 3174
BspANI GGCC 8 cut(s) 395, 435, 2167, 2538, 3504, 3592, 3957, 4118
BspCNI CTCAG 6 cut(s) 1635, 1849, 2088, 2886, 3927, 3999
BspDI ATCGAT 1 cut(s) 2312
BspFNI CGCG 1 cut(s) 2814
BspHI TCATGA 3 cut(s) 1137, 2749, 2916
BspLI GGNNCC 3 cut(s) 1898, 2167, 2168
BspMI ACCTGC 2 cut(s) 3265, 4038
BspPI GGATC 3 cut(s) 562, 1069, 2399
BspT104I TTCGAA 1 cut(s) 3022
BspT107I GGYRCC 1 cut(s) 1896
BspTI CTTAAG 1 cut(s) 779
BspTNI GGTCTC 3 cut(s) 1057, 1150, 2576
BsrDI GCAATG 3 cut(s) 2273, 3064, 3238
BsrI ACTGG 7 cut(s) 2615, 2769, 2911, 3244, 3417, 3797, 4118
BssECI CCNNGG 6 cut(s) 276, 396, 762, 1215, 2632, 3952
BssNI GRCGYC 2 cut(s) 273, 3710
BssSI CACGAG 2 cut(s) 177, 1035
BssT1I CCWWGG 3 cut(s) 396, 1215, 2632
Bst2BI CACGAG 2 cut(s) 177, 1035
Bst2UI CCWGG 5 cut(s) 708, 764, 1011, 3677, 3953
Bst6I CTCTTC 2 cut(s) 2181, 4042
BstACI GRCGYC 2 cut(s) 273, 3710
BstAFI CTTAAG 1 cut(s) 779
BstAPI GCANNNNNTGC 2 cut(s) 1953, 2113
BstBAI YACGTR 1 cut(s) 2422
BstBI TTCGAA 1 cut(s) 3022
BstC8I GCNNGC 7 cut(s) 415, 593, 704, 1131, 3141, 3800, 4011
BstDEI CTNAG 9 cut(s) 1622, 1857, 2087, 2096, 2894, 3218, 3935, 3986, 4079
BstENI CCTNNNNNAGG 1 cut(s) 2631
BstF5I GGATG 7 cut(s) 325, 566, 946, 1446, 2236, 3353, 4013
BstFNI CGCG 1 cut(s) 2814
BstHHI GCGC 1 cut(s) 2814
BstMAI GTCTC 9 cut(s) 993, 1057, 1150, 1625, 2178, 2576, 3410, 3931, 4146
BstMCI CGRYCG 1 cut(s) 2316
BstMWI GCNNNNNNNGC 6 cut(s) 257, 1601, 1953, 2083, 2113, 3589
BstNI CCWGG 5 cut(s) 708, 764, 1011, 3677, 3953
BstNSI RCATGY 7 cut(s) 301, 382, 529, 706, 1133, 2329, 2432
BstSCI CCNGG 6 cut(s) 706, 762, 1009, 1681, 3675, 3951
BstSFI CTRYAG 2 cut(s) 983, 2785
BstSLI GKGCMC 2 cut(s) 2169, 2847
BstUI CGCG 1 cut(s) 2814
BstV1I GCAGC 7 cut(s) 127, 1138, 1207, 1591, 2290, 2988, 3873
BstV2I GAAGAC 7 cut(s) 630, 725, 1260, 1494, 1767, 2003, 3503
BstX2I RGATCY 3 cut(s) 554, 1074, 2404
BstXI CCANNNNNNTGG 1 cut(s) 4113
BstYI RGATCY 3 cut(s) 554, 1074, 2404
Bsu15I ATCGAT 1 cut(s) 2312
BsuRI GGCC 8 cut(s) 395, 435, 2167, 2538, 3504, 3592, 3957, 4118
BsuTUI ATCGAT 1 cut(s) 2312
BtrI CACGTC 3 cut(s) 852, 1664, 3186
BtsCI GGATG 7 cut(s) 325, 566, 946, 1446, 2236, 3353, 4013
BtsI GCAGTG 3 cut(s) 126, 267, 2000
BveI ACCTGC 2 cut(s) 3265, 4038
Cac8I GCNNGC 7 cut(s) 415, 593, 704, 1131, 3141, 3800, 4011
CaiI CAGNNNCTG 2 cut(s) 800, 3682
CciI TCATGA 3 cut(s) 1137, 2749, 2916
CfoI GCGC 1 cut(s) 2814
Cfr13I GGNCC 8 cut(s) 602, 2165, 2166, 3502, 3591, 3955, 4027, 4116
ClaI ATCGAT 1 cut(s) 2312
CseI GACGC 2 cut(s) 281, 3699
Csp6I GTAC 8 cut(s) 23, 428, 669, 1740, 1829, 1897, 3313, 4138
CviQI GTAC 8 cut(s) 23, 428, 669, 1740, 1829, 1897, 3313, 4138
DdeI CTNAG 9 cut(s) 1622, 1857, 2087, 2096, 2894, 3218, 3935, 3986, 4079
DraI TTTAAA 1 cut(s) 2052
DraIII CACNNNGTG 1 cut(s) 1327
Eam1104I CTCTTC 2 cut(s) 2181, 4042
EarI CTCTTC 2 cut(s) 2181, 4042
EciI GGCGGA 1 cut(s) 1221
Eco130I CCWWGG 3 cut(s) 396, 1215, 2632
Eco147I AGGCCT 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 2169
Eco31I GGTCTC 3 cut(s) 1057, 1150, 2576
Eco32I GATATC 2 cut(s) 2639, 3015
Eco47I GGWCC 2 cut(s) 602, 4027
Eco57I CTGAAG 4 cut(s) 134, 1743, 2015, 3447
Eco88I CYCGRG 1 cut(s) 3211
EcoNI CCTNNNNNAGG 1 cut(s) 2631
EcoO109I RGGNCCY 6 cut(s) 2165, 2166, 3502, 3591, 3955, 4027
EcoRI GAATTC 2 cut(s) 875, 1867
EcoRII CCWGG 5 cut(s) 706, 762, 1009, 3675, 3951
EcoRV GATATC 2 cut(s) 2639, 3015
EcoT14I CCWWGG 3 cut(s) 396, 1215, 2632
EcoT22I ATGCAT 4 cut(s) 1135, 1575, 2275, 3839
EcoT38I GRGCYC 1 cut(s) 2169
ErhI CCWWGG 3 cut(s) 396, 1215, 2632
Esp3I CGTCTC 1 cut(s) 3931
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FaqI GGGAC 2 cut(s) 2200, 2676
FauI CCCGC 2 cut(s) 209, 1600
FbaI TGATCA 3 cut(s) 967, 3657, 3850
Fnu4HI GCNGC 8 cut(s) 141, 564, 1127, 1221, 1605, 2279, 3002, 3887
FokI GGATG 7 cut(s) 332, 553, 953, 1453, 2243, 3340, 4000
FriOI GRGCYC 1 cut(s) 2169
Fsp4HI GCNGC 8 cut(s) 141, 564, 1127, 1221, 1605, 2279, 3002, 3887
FspBI CTAG 9 cut(s) 26, 558, 666, 1524, 1989, 2540, 2648, 3113, 3719
GlaI GCGC 1 cut(s) 2813
GluI GCNGC 8 cut(s) 141, 564, 1127, 1221, 1605, 2279, 3002, 3887
GsaI CCCAGC 1 cut(s) 3771
GsuI CTGGAG 4 cut(s) 729, 2928, 3434, 3659
HaeIII GGCC 8 cut(s) 395, 435, 2167, 2538, 3504, 3592, 3957, 4118
HapII CCGG 3 cut(s) 1683, 2922, 3497
HgaI GACGC 2 cut(s) 281, 3699
HhaI GCGC 1 cut(s) 2814
Hin1I GRCGYC 2 cut(s) 273, 3710
Hin6I GCGC 1 cut(s) 2812
HinP1I GCGC 1 cut(s) 2812
HincII GTYRAC 4 cut(s) 1147, 1702, 2093, 2733
HindII GTYRAC 4 cut(s) 1147, 1702, 2093, 2733
HindIII AAGCTT 4 cut(s) 221, 2720, 2738, 4007
HpaI GTTAAC 2 cut(s) 1702, 2733
HpaII CCGG 3 cut(s) 1683, 2922, 3497
HphI GGTGA 8 cut(s) 1313, 1568, 1598, 2717, 2972, 3697, 3808, 3961
Hpy166II GTNNAC 8 cut(s) 962, 1147, 1454, 1702, 2093, 2733, 2845, 3407
Hpy8I GTNNAC 8 cut(s) 962, 1147, 1454, 1702, 2093, 2733, 2845, 3407
HpyCH4IV ACGT 7 cut(s) 851, 1663, 1732, 2111, 2421, 2808, 3185
HpyF10VI GCNNNNNNNGC 6 cut(s) 257, 1601, 1953, 2083, 2113, 3589
HpyF3I CTNAG 9 cut(s) 1622, 1857, 2087, 2096, 2894, 3218, 3935, 3986, 4079
HpySE526I ACGT 7 cut(s) 851, 1663, 1732, 2111, 2421, 2808, 3185
Hsp92I GRCGYC 2 cut(s) 273, 3710
HspAI GCGC 1 cut(s) 2812
KpnI GGTACC 1 cut(s) 1900
Ksp22I TGATCA 3 cut(s) 967, 3657, 3850
KspAI GTTAAC 2 cut(s) 1702, 2733
LmnI GCTCC 6 cut(s) 400, 548, 1004, 3271, 3293, 3679
Lsp1109I GCAGC 7 cut(s) 127, 1138, 1207, 1591, 2290, 2988, 3873
LweI GCATC 8 cut(s) 575, 1582, 1897, 2004, 2221, 2260, 3092, 4022
MaeI CTAG 9 cut(s) 26, 558, 666, 1524, 1989, 2540, 2648, 3113, 3719
MaeII ACGT 7 cut(s) 851, 1663, 1732, 2111, 2421, 2808, 3185
MfeI CAATTG 9 cut(s) 693, 1424, 1872, 2452, 2480, 2775, 2942, 3059, 3772
MflI RGATCY 3 cut(s) 554, 1074, 2404
MhlI GDGCHC 3 cut(s) 1009, 2169, 2847
MlyI GAGTC 8 cut(s) 1027, 1222, 1306, 1321, 2138, 3137, 3223, 3807
MmeI TCCRAC 4 cut(s) 592, 982, 1608, 3621
Mph1103I ATGCAT 4 cut(s) 1135, 1575, 2275, 3839
MroXI GAANNNNTTC 5 cut(s) 1119, 1176, 1555, 3459, 3641
MslI CAYNNNNRTG 6 cut(s) 296, 302, 377, 854, 1435, 2977
MspA1I CMGCKG 1 cut(s) 2281
MspCI CTTAAG 1 cut(s) 779
MspI CCGG 3 cut(s) 1683, 2922, 3497
MspR9I CCNGG 6 cut(s) 708, 764, 1011, 1683, 3677, 3953
MunI CAATTG 9 cut(s) 693, 1424, 1872, 2452, 2480, 2775, 2942, 3059, 3772
Mva1269I GAATGC 3 cut(s) 1133, 1715, 2079
MvaI CCWGG 5 cut(s) 708, 764, 1011, 3677, 3953
MvnI CGCG 1 cut(s) 2814
MwoI GCNNNNNNNGC 6 cut(s) 257, 1601, 1953, 2083, 2113, 3589
NciI CCSGG 1 cut(s) 1683
NlaIV GGNNCC 3 cut(s) 1898, 2167, 2168
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 9 cut(s) 66, 847, 863, 1313, 1499, 1777, 2141, 2243, 4127
NsiI ATGCAT 4 cut(s) 1135, 1575, 2275, 3839
NspI RCATGY 7 cut(s) 301, 382, 529, 706, 1133, 2329, 2432
NspV TTCGAA 1 cut(s) 3022
OliI CACNNNNGTG 2 cut(s) 854, 1435
PaeI GCATGC 2 cut(s) 706, 1133
PagI TCATGA 3 cut(s) 1137, 2749, 2916
PaqCI CACCTGC 1 cut(s) 3265
PceI AGGCCT 1 cut(s) 435
PciI ACATGT 2 cut(s) 378, 2325
PcsI WCGNNNNNNNCGW 1 cut(s) 1660
PctI GAATGC 3 cut(s) 1133, 1715, 2079
PdmI GAANNNNTTC 5 cut(s) 1119, 1176, 1555, 3459, 3641
PflMI CCANNNNNTGG 2 cut(s) 2474, 3682
PfoI TCCNGGA 1 cut(s) 3675
PkrI GCNGC 8 cut(s) 142, 565, 1128, 1222, 1606, 2280, 3003, 3888
Ple19I CGATCG 1 cut(s) 2316
PleI GAGTC 8 cut(s) 1027, 1222, 1305, 1320, 2138, 3136, 3222, 3807
PpsI GAGTC 8 cut(s) 1027, 1222, 1305, 1320, 2138, 3136, 3222, 3807
Ppu21I YACGTR 1 cut(s) 2422
PpuMI RGGWCCY 1 cut(s) 4027
PscI ACATGT 2 cut(s) 378, 2325
PshBI ATTAAT 2 cut(s) 1919, 1970
Psp1406I AACGTT 1 cut(s) 2111
Psp5II RGGWCCY 1 cut(s) 4027
Psp6I CCWGG 5 cut(s) 706, 762, 1009, 3675, 3951
PspFI CCCAGC 1 cut(s) 3767
PspGI CCWGG 5 cut(s) 706, 762, 1009, 3675, 3951
PspN4I GGNNCC 3 cut(s) 1898, 2167, 2168
PspOMI GGGCCC 1 cut(s) 2165
PspPI GGNCC 8 cut(s) 602, 2165, 2166, 3502, 3591, 3955, 4027, 4116
PspPPI RGGWCCY 1 cut(s) 4027
PstNI CAGNNNCTG 2 cut(s) 800, 3682
PsuI RGATCY 3 cut(s) 554, 1074, 2404
PvuI CGATCG 1 cut(s) 2316
PvuII CAGCTG 1 cut(s) 2281
RsaI GTAC 8 cut(s) 24, 429, 670, 1741, 1830, 1898, 3314, 4139
RsaNI GTAC 8 cut(s) 23, 428, 669, 1740, 1829, 1897, 3313, 4138
RseI CAYNNNNRTG 6 cut(s) 296, 302, 377, 854, 1435, 2977
SatI GCNGC 8 cut(s) 141, 564, 1127, 1221, 1605, 2279, 3002, 3887
Sau96I GGNCC 8 cut(s) 602, 2165, 2166, 3502, 3591, 3955, 4027, 4116
ScaI AGTACT 2 cut(s) 670, 3314
SchI GAGTC 8 cut(s) 1027, 1222, 1306, 1321, 2138, 3137, 3223, 3807
ScrFI CCNGG 6 cut(s) 708, 764, 1011, 1683, 3677, 3953
SduI GDGCHC 3 cut(s) 1009, 2169, 2847
SfaNI GCATC 8 cut(s) 575, 1582, 1897, 2004, 2221, 2260, 3092, 4022
SfcI CTRYAG 2 cut(s) 983, 2785
SfuI TTCGAA 1 cut(s) 3022
SinI GGWCC 2 cut(s) 602, 4027
SmiI ATTTAAAT 1 cut(s) 2052
SmiMI CAYNNNNRTG 6 cut(s) 296, 302, 377, 854, 1435, 2977
SmlI CTYRAG 8 cut(s) 779, 898, 2146, 2200, 2741, 2861, 3308, 3316
SmoI CTYRAG 8 cut(s) 779, 898, 2146, 2200, 2741, 2861, 3308, 3316
SpeI ACTAGT 1 cut(s) 25
SphI GCATGC 2 cut(s) 706, 1133
SseBI AGGCCT 1 cut(s) 435
SsiI CCGC 6 cut(s) 216, 546, 563, 1206, 1607, 3174
SspI AATATT 4 cut(s) 757, 1278, 1974, 3257
SspMI CTAG 9 cut(s) 26, 558, 666, 1524, 1989, 2540, 2648, 3113, 3719
StuI AGGCCT 1 cut(s) 435
StyD4I CCNGG 6 cut(s) 706, 762, 1009, 1681, 3675, 3951
StyI CCWWGG 3 cut(s) 396, 1215, 2632
SwaI ATTTAAAT 1 cut(s) 2052
TaiI ACGT 7 cut(s) 854, 1666, 1735, 2114, 2424, 2811, 3188
TaqII GACCGA 1 cut(s) 835
TatI WGTACW 3 cut(s) 22, 668, 3312
TauI GCSGC 1 cut(s) 566
TseFI GTSAC 9 cut(s) 66, 847, 863, 1313, 1499, 1777, 2141, 2243, 4127
TseI GCWGC 7 cut(s) 140, 1126, 1220, 1604, 2278, 3001, 3886
Tsp45I GTSAC 9 cut(s) 66, 847, 863, 1313, 1499, 1777, 2141, 2243, 4127
TspGWI ACGGA 1 cut(s) 4125
Van91I CCANNNNNTGG 2 cut(s) 2474, 3682
Vha464I CTTAAG 1 cut(s) 779
VneI GTGCAC 1 cut(s) 2843
VpaK11BI GGWCC 2 cut(s) 602, 4027
VspI ATTAAT 2 cut(s) 1919, 1970
XagI CCTNNNNNAGG 1 cut(s) 2631
XapI RAATTY 9 cut(s) 191, 875, 1172, 1409, 1614, 1706, 1867, 2119, 3488
XbaI TCTAGA 2 cut(s) 1988, 3718
XceI RCATGY 7 cut(s) 301, 382, 529, 706, 1133, 2329, 2432
XcmI CCANNNNNNNNNTGG 1 cut(s) 3789
XmnI GAANNNNTTC 5 cut(s) 1119, 1176, 1555, 3459, 3641
XspI CTAG 9 cut(s) 26, 558, 666, 1524, 1989, 2540, 2648, 3113, 3719
ZrmI AGTACT 2 cut(s) 670, 3314
Zsp2I ATGCAT 4 cut(s) 1135, 1575, 2275, 3839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.