MD11G1011400.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
841416 .. 844981
3566 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1011400.v1.1.491

Sequence Viewer

Length: 3294 bp
ATGTTGAAACGTCAGATACAACATGCTCATTCCAGCACAATAAGCAAAGCATGGAAATCAATTCCTACTCGTGTTTCCAACTTCAAAACAACAAGCAAAGCATGGAAATCAATTCCTAATCGTGTTTTCAACTTCAAAATGAACTCGGAAATACAAAAGATTACCAAGAGATTACAAGAGATATCCGAACGAAAAGACCAGCTTGGTTTGAAAATTGATATTGGGACGTTGACTACAAGGGCACACCAACACATATCGCCTAGTTCTAGTCTACCGGATGGACCTGTGATTGGAAGGGACGAGGACAAAAGGCAGATTGTTGAGCTGTTGTCGAAACAAGAGCATCATGCTGTCAATTTCGATGTAGTTGCAATTGTTGGTATGGCTGGAGTCGGAAAGACAACACTTGCTGGACAATCCCCTTTTCGTGATGGAGCACAAGGAAGCAAGATAATTGTGACAGCACGTGATACAGATGTTTCAAAAATGATGGGAGTTGCCAAGCTGGTTCACAATTTGGAGCCCATGCAAAATGATGTTTGTTTGCAAGTATTTGAGCAGCATGCATTCTTAAATTCTAACAACGACAAACCACCAAATTACGAGTTACTTAAGGAGAAAATTGTTGCGAAGTGTAGGGGATTGCCTTTGGCCGCGAGGACCCTCGGTGGTGTTCTACTTTGTAAAGAAACATACGAATGGGAAGAAATATTGAACAACAAACTGTGGAGTCTATCAAATGAGCAAGACATACTTCCAGTATTGAGATTAACCTACTTTTATCTTCCTTCACATTTGAAAAGATGTTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTCGAAGAGAAGCAAATGATCCTTCTATGGATGGCCGAGGGCTTTATTCTTCCCCGACCAGAAGGTAATAAGCAAATTGTAGATTTACGTACTAATTATTTTCGGGAGCTCGTATCTAGGTCATTGTTTCAAAAGTCAACAAAAAATACTTCAAAATATGTGATGCATGACCTCATTGGTGATTTAGCGCGATGGGCAGCTGGAGATATTTGTTTTAGATTGGAGGATAATCAAAATGATGATGGTGTACAACTTAGATGTTTTCCGAAGGCACGCCATTCGTCTTACATCATGGGTCGGTTTGATGGGGTTAAAAGATTTGAGGCATTTTCTGAAGTGAAATGTTTGCGAACCTTCCTGCCACTAACAAAGGGTGATATTCGGAGTTATTTAAGTCATCAGGTTCCTTTTGATTTATTGCCAAAATTGCAATACTTGCGGGTGCTCTCTTTTAATGGCTATAAAATAACTAAGCTTCCAAACTTAGTCGGTGATTTAAGGTATCTACGGTATCTCGACCTTTCTTACGCGCTCATTACGTCTTTACCTAAATCAACAAGCACTCTTTACAACCTGCAGGCATTGATATTGAAAGGCTGTCATCAGTTGAAGTCATTGCCTGCAGACATGAGTAATCTAATTAATTTGCGTCATCTCAACAACTCATATGCATATTCGGTGGAAGGAATGCCTCCAAAACTAGGTAAATTGGTGAATCTCCAATCTTTGCCTAATTTTGTGGTGAGCGGTGGTAGTAATCAATCAGGGATAAGAGAGATAGAGTTCTTGATGCATCTCCGAAGGACATTGTGCCTCTCAAGATTGGAGAATGTGACTGATGTCGAGGACGCTCGGAGGGCCAACTTAAAATGCAAGGAGAGGCTTGATTCGTTGGTCCTAGAATGGTCTCATTCAAGCGACACAAGAGAAACGAATTTCGTTGTGCTTGACATGTTACGGCCTCATACAAAGGTCAAGGAGCTCACCATCAAAAGTTATGCTGGAAAAGAATTTTCATCATGGGTTGGAAGTTCTTTGTTCTCTAATATGGCAGTCGTGCACTTAGAGGAATGCAACAATTGTTTATCGTTGCCACCTCTCGGACAATTGCCTCATCTCAAAGAACTTTATATTCGAGGAATGAATGCGGTGGAAAGTGTTGGTGCTGAGTTTTATGGAGAGTGTATCTTTCCTTTTCCGCTATTAGAGACTATTACGTTTGTGAATATGAAGCATTGGAAGGTATGGCTTCCCTTCCAACATGATCGAGGAAGTGGTGATTTCCCTTGCCTGAAAAGGCTTTCAATAAAAAAATGTTCTAAGTTGGAAGGTAAGCTGCCAGAGAACCTTGATCTGTTAGCCGAGCTTGAAATTTTTAAATGTGAGGAATTGGTGGTTTCGATTACCAACTGCAAACAACTTCGTCAAATAAACATTGACGGTTGTAAAGCGTTGGTGCATACAGCTACTAAGGTTGAGTTTGAGTTATTAAAGTCCTTGTGTCTTTCAAACATTTCGAAGTTGATGTCTCTGCAAACAAGGGAATTGTGCAGAAAGGGATTAACCAAGGTTAGAGAATTGAAGATTAATGGATATAAGGAGCTGAAGTCTTCATTGAAGAATGAGGCTGGATTATTGCAGCAGTTGACTTCTCTTGGCCGTTTGAAGATCGAAGACAACTCTCCTCTAGTTGAAGAATTGGGAAAAGAAGCAGAGGAGTTGCAAATATTGGAGTGCAGGATTGAATGTATGGAGTTAATCAAGTGCGAAAATCATTTGAAGCTACCAAAAGGGTTAAATCAGTTGTCGTCTCTTCAAGAGCTTCGCTTACACAAATGTTCACGTTTAGCTTCTTTTCCAGATGTTGGTCTGCCACCTTCTCTTAAACACATCCAGATTACCGAGTGTCATTCGTTGATATATTTGGCAAAACATCAGATTCTCCAAAATCTCAGAAGAATAGAGATAAGAGAGTGCAAAAGTTTGAAATCACTAGTAGATGAGAAGGATGTTGGTTCTTCCCCATCGTCTTCTTCTCATAGTTGTCTTGAGTACTTGAATATTGAAGAATGTGAATCTCTGACGTCGTTATCATTGAGTGGCCAGCTTCTCAGGACGCTTAAACACCTTGGGATATTTTATTGTGAACAACTGGAGCTAATCGCAGAGGACGGGTTTTTCCGCGACAACACTAATTATTGTCTTGAATATATTAAGATCTGGAAATGCCAAAATCTGAAATCCTTACCGGATGGCTTATGCCACCTCAGCAATCTTCAAACTCTAATTATTGGGAACTGTGGAACACTACTACAAAAGGGCCTTATAGTGTCAGTGGGTGGCGTGAGAAAGATCTATATCAGAGATTGTGAGAAATTGGAGGCGTTGCCCGAAGACATGCACAATCTTAACTCTCTTGAGGAATTGAGCATCGACTACCGTGAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1098

Amino Acids

124.92

Weight (kDa)

8.63

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 275 - 344 9.1e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 409 - 698 8.7e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 538 - 662 8.8e-36 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 958 - 1093 3.6e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2930
Acc36I ACCTGC 1 cut(s) 1425
AccB7I CCANNNNNTGG 1 cut(s) 2708
AccBSI CCGCTC 1 cut(s) 1590
AccI GTMKAC 1 cut(s) 271
AccII CGCG 4 cut(s) 656, 1033, 1373, 3027
AciI CCGC 6 cut(s) 654, 1282, 1590, 1991, 2042, 3025
AclWI GGATC 1 cut(s) 856
AcoI YGGCCR 4 cut(s) 651, 876, 2500, 2944
AcsI RAATTY 5 cut(s) 574, 842, 1777, 1853, 2214
AcuI CTGAAG 2 cut(s) 1197, 2468
AcvI CACGTG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 2927
AfaI GTAC 3 cut(s) 934, 1092, 2897
AfiI CCNNNNNNNGG 4 cut(s) 290, 1544, 1943, 2708
AflII CTTAAG 1 cut(s) 611
AflIII ACRYGT 1 cut(s) 1794
AhlI ACTAGT 1 cut(s) 2836
Alw21I GWGCWC 5 cut(s) 439, 954, 1290, 1827, 1905
Alw26I GTCTC 4 cut(s) 1755, 2045, 2376, 2658
Alw44I GTGCAC 1 cut(s) 1901
AlwI GGATC 1 cut(s) 856
AoxI GGCC 7 cut(s) 651, 876, 1701, 1802, 2500, 2944, 3163
ApaLI GTGCAC 1 cut(s) 1901
ApeKI GCWGC 4 cut(s) 559, 1040, 2179, 2482
ApoI RAATTY 5 cut(s) 574, 842, 1777, 1853, 2214
ArsI GACNNNNNNTTYG 4 cut(s) 827, 859, 2354, 2386
AseI ATTAAT 2 cut(s) 1485, 2430
AspLEI GCGC 2 cut(s) 1033, 1375
AspS9I GGNCC 5 cut(s) 281, 660, 1701, 1738, 3163
AsuHPI GGTGA 7 cut(s) 1034, 1229, 1346, 1567, 1597, 1819, 2132
AsuII TTCGAA 2 cut(s) 846, 2360
AvaII GGWCC 3 cut(s) 281, 660, 1738
BaeGI GKGCMC 2 cut(s) 244, 1905
BaeI ACNNNNGTAYC 2 cut(s) 462, 495
BalI TGGCCA 1 cut(s) 2946
BanII GRGCYC 3 cut(s) 525, 954, 1827
BauI CACGAG 1 cut(s) 69
BbrPI CACGTG 1 cut(s) 467
BbsI GAAGAC 4 cut(s) 2445, 2523, 2865, 3243
Bbv12I GWGCWC 5 cut(s) 439, 954, 1290, 1827, 1905
BbvCI CCTCAGC 1 cut(s) 3110
BbvI GCAGC 4 cut(s) 571, 1052, 2166, 2494
BceAI ACGGC 2 cut(s) 1817, 2487
BcgI CGANNNNNNTGC 4 cut(s) 350, 384, 1104, 1138
BcoDI GTCTC 4 cut(s) 1755, 2045, 2376, 2658
BcuI ACTAGT 1 cut(s) 2836
BfaI CTAG 7 cut(s) 261, 267, 960, 1544, 1742, 2531, 2837
BfmI CTRYAG 2 cut(s) 1418, 1464
BfrI CTTAAG 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 1425
BglII AGATCT 2 cut(s) 3060, 3195
BisI GCNGC 5 cut(s) 560, 654, 1041, 2180, 2483
BlsI GCNGC 5 cut(s) 561, 655, 1042, 2181, 2484
BmcAI AGTACT 1 cut(s) 2897
Bme18I GGWCC 3 cut(s) 281, 660, 1738
BmgT120I GGNCC 5 cut(s) 281, 660, 1701, 1738, 3163
BmiI GGNNCC 3 cut(s) 522, 662, 1248
BmsI GCATC 5 cut(s) 352, 996, 1623, 1645, 3282
BoxI GACNNNNGTC 1 cut(s) 1682
BpiI GAAGAC 4 cut(s) 2445, 2523, 2865, 3243
BpmI CTGGAG 3 cut(s) 408, 1065, 3017
Bpu10I CCTNAGC 1 cut(s) 3110
Bpu14I TTCGAA 2 cut(s) 846, 2360
BpuEI CTTGAG 3 cut(s) 1645, 2912, 3281
BsaAI YACGTR 2 cut(s) 467, 932
BsaBI GATNNNNATC 1 cut(s) 3200
BsaHI GRCGYC 1 cut(s) 2927
BsaI GGTCTC 1 cut(s) 1755
BsaJI CCNNGG 4 cut(s) 664, 879, 2409, 2971
BsaWI WCCGGW 2 cut(s) 274, 3091
BsaXI ACNNNNNCTCC 2 cut(s) 2435, 2465
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 1544, 1943, 2708
Bse1I ACTGG 2 cut(s) 758, 3000
Bse3DI GCAATG 1 cut(s) 1457
Bse8I GATNNNNATC 1 cut(s) 3200
BseDI CCNNGG 4 cut(s) 664, 879, 2409, 2971
BseGI GGATG 5 cut(s) 283, 879, 2733, 2857, 3100
BseJI GATNNNNATC 1 cut(s) 3200
BseLI CCNNNNNNNGG 4 cut(s) 290, 1544, 1943, 2708
BseMI GCAATG 1 cut(s) 1457
BseMII CTCAG 4 cut(s) 2001, 2809, 2968, 3124
BseNI ACTGG 2 cut(s) 758, 3000
BseRI GAGGAG 2 cut(s) 2517, 2573
BseSI GKGCMC 2 cut(s) 244, 1905
BseXI GCAGC 4 cut(s) 571, 1052, 2166, 2494
BsgI GTGCAG 2 cut(s) 2413, 2599
Bsh1236I CGCG 4 cut(s) 656, 1033, 1373, 3027
BshFI GGCC 7 cut(s) 653, 878, 1703, 1804, 2502, 2946, 3165
BsiHKAI GWGCWC 5 cut(s) 439, 954, 1290, 1827, 1905
BsiSI CCGG 2 cut(s) 275, 3092
BslFI GGGAC 2 cut(s) 238, 311
BslI CCNNNNNNNGG 4 cut(s) 290, 1544, 1943, 2708
BsmAI GTCTC 4 cut(s) 1755, 2045, 2376, 2658
BsmBI CGTCTC 1 cut(s) 2658
BsmFI GGGAC 2 cut(s) 238, 311
BsmI GAATGC 4 cut(s) 566, 1536, 1919, 1993
BsnI GGCC 7 cut(s) 653, 878, 1703, 1804, 2502, 2946, 3165
Bso31I GGTCTC 1 cut(s) 1755
Bsp119I TTCGAA 2 cut(s) 846, 2360
Bsp1286I GDGCHC 7 cut(s) 244, 439, 525, 954, 1290, 1827, 1905
Bsp1407I TGTACA 1 cut(s) 1090
Bsp143I GATC 6 cut(s) 861, 2107, 2194, 2511, 3060, 3195
BspACI CCGC 6 cut(s) 654, 1282, 1590, 1991, 2042, 3025
BspANI GGCC 7 cut(s) 653, 878, 1703, 1804, 2502, 2946, 3165
BspCNI CTCAG 4 cut(s) 2002, 2808, 2967, 3123
BspFNI CGCG 4 cut(s) 656, 1033, 1373, 3027
BspLI GGNNCC 3 cut(s) 522, 662, 1248
BspMAI CTGCAG 2 cut(s) 1422, 1468
BspMI ACCTGC 1 cut(s) 1425
BspPI GGATC 1 cut(s) 856
BspT104I TTCGAA 2 cut(s) 846, 2360
BspTI CTTAAG 1 cut(s) 611
BspTNI GGTCTC 1 cut(s) 1755
BsrBI CCGCTC 1 cut(s) 1590
BsrDI GCAATG 1 cut(s) 1457
BsrGI TGTACA 1 cut(s) 1090
BsrI ACTGG 2 cut(s) 758, 3000
BssECI CCNNGG 4 cut(s) 664, 879, 2409, 2971
BssMI GATC 6 cut(s) 861, 2107, 2194, 2511, 3060, 3195
BssNI GRCGYC 1 cut(s) 2927
BssSI CACGAG 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 2409, 2971
Bst2BI CACGAG 1 cut(s) 69
Bst4CI ACNGT 5 cut(s) 726, 1353, 2285, 3143, 3284
Bst6I CTCTTC 2 cut(s) 843, 2661
BstACI GRCGYC 1 cut(s) 2927
BstAFI CTTAAG 1 cut(s) 611
BstAPI GCANNNNNTGC 1 cut(s) 1279
BstAUI TGTACA 1 cut(s) 1090
BstBAI YACGTR 2 cut(s) 467, 932
BstBI TTCGAA 2 cut(s) 846, 2360
BstC8I GCNNGC 5 cut(s) 564, 1117, 1422, 1464, 2948
BstF5I GGATG 5 cut(s) 283, 879, 2733, 2857, 3100
BstFNI CGCG 4 cut(s) 656, 1033, 1373, 3027
BstHHI GCGC 2 cut(s) 1033, 1375
BstKTI GATC 6 cut(s) 864, 2110, 2197, 2514, 3063, 3198
BstMAI GTCTC 4 cut(s) 1755, 2045, 2376, 2658
BstMBI GATC 6 cut(s) 861, 2107, 2194, 2511, 3060, 3195
BstMWI GCNNNNNNNGC 6 cut(s) 42, 1037, 1270, 1279, 1700, 3111
BstNSI RCATGY 4 cut(s) 26, 566, 1798, 3244
BstPAI GACNNNNGTC 1 cut(s) 1682
BstSFI CTRYAG 2 cut(s) 1418, 1464
BstSLI GKGCMC 2 cut(s) 244, 1905
BstSNI TACGTA 1 cut(s) 932
BstUI CGCG 4 cut(s) 656, 1033, 1373, 3027
BstV1I GCAGC 4 cut(s) 571, 1052, 2166, 2494
BstV2I GAAGAC 4 cut(s) 2445, 2523, 2865, 3243
BstX2I RGATCY 2 cut(s) 3060, 3195
BstYI RGATCY 2 cut(s) 3060, 3195
BsuRI GGCC 7 cut(s) 653, 878, 1703, 1804, 2502, 2946, 3165
BtgZI GCGATG 1 cut(s) 1048
BtsCI GGATG 5 cut(s) 283, 879, 2733, 2857, 3100
BtsIMutI CAGTG 1 cut(s) 3183
BveI ACCTGC 1 cut(s) 1425
Cac8I GCNNGC 5 cut(s) 564, 1117, 1422, 1464, 2948
CfoI GCGC 2 cut(s) 1033, 1375
Cfr13I GGNCC 5 cut(s) 281, 660, 1701, 1738, 3163
CseI GACGC 3 cut(s) 1481, 1700, 2968
Csp6I GTAC 3 cut(s) 933, 1091, 2896
CviQI GTAC 3 cut(s) 933, 1091, 2896
DpnI GATC 6 cut(s) 863, 2109, 2196, 2513, 3062, 3197
DpnII GATC 6 cut(s) 861, 2107, 2194, 2511, 3060, 3195
DraI TTTAAA 1 cut(s) 2221
EaeI YGGCCR 4 cut(s) 651, 876, 2500, 2944
Eam1104I CTCTTC 2 cut(s) 843, 2661
EarI CTCTTC 2 cut(s) 843, 2661
Ecl136II GAGCTC 2 cut(s) 952, 1825
Eco105I TACGTA 1 cut(s) 932
Eco130I CCWWGG 2 cut(s) 2409, 2971
Eco24I GRGCYC 3 cut(s) 525, 954, 1827
Eco31I GGTCTC 1 cut(s) 1755
Eco32I GATATC 1 cut(s) 183
Eco47I GGWCC 3 cut(s) 281, 660, 1738
Eco53kI GAGCTC 2 cut(s) 952, 1825
Eco57I CTGAAG 2 cut(s) 1197, 2468
Eco72I CACGTG 1 cut(s) 467
EcoICRI GAGCTC 2 cut(s) 952, 1825
EcoO109I RGGNCCY 2 cut(s) 660, 3163
EcoRI GAATTC 1 cut(s) 842
EcoRV GATATC 1 cut(s) 183
EcoT14I CCWWGG 2 cut(s) 2409, 2971
EcoT22I ATGCAT 4 cut(s) 568, 1011, 1516, 1638
EcoT38I GRGCYC 3 cut(s) 525, 954, 1827
ErhI CCWWGG 2 cut(s) 2409, 2971
Esp3I CGTCTC 1 cut(s) 2658
FalI AAGNNNNNCTT 4 cut(s) 186, 218, 738, 770
FaqI GGGAC 2 cut(s) 238, 311
FauI CCCGC 1 cut(s) 1275
FauNDI CATATG 1 cut(s) 1510
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 5 cut(s) 560, 654, 1041, 2180, 2483
FokI GGATG 5 cut(s) 290, 886, 2720, 2864, 3107
FriOI GRGCYC 3 cut(s) 525, 954, 1827
Fsp4HI GCNGC 5 cut(s) 560, 654, 1041, 2180, 2483
FspBI CTAG 7 cut(s) 261, 267, 960, 1544, 1742, 2531, 2837
GlaI GCGC 2 cut(s) 1032, 1374
GluI GCNGC 5 cut(s) 560, 654, 1041, 2180, 2483
GsuI CTGGAG 3 cut(s) 408, 1065, 3017
HaeIII GGCC 7 cut(s) 653, 878, 1703, 1804, 2502, 2946, 3165
HapII CCGG 2 cut(s) 275, 3092
HgaI GACGC 3 cut(s) 1481, 1700, 2968
HhaI GCGC 2 cut(s) 1033, 1375
Hin1I GRCGYC 1 cut(s) 2927
Hin6I GCGC 2 cut(s) 1031, 1373
HinP1I GCGC 2 cut(s) 1031, 1373
HincII GTYRAC 3 cut(s) 231, 981, 2490
HindII GTYRAC 3 cut(s) 231, 981, 2490
HindIII AAGCTT 1 cut(s) 1316
HinfI GANTC 6 cut(s) 390, 730, 1558, 1730, 2782, 2918
HpaII CCGG 2 cut(s) 275, 3092
HphI GGTGA 7 cut(s) 1034, 1229, 1346, 1567, 1597, 1819, 2132
Hpy166II GTNNAC 9 cut(s) 231, 272, 511, 981, 1091, 1903, 2490, 2684, 2990
Hpy8I GTNNAC 9 cut(s) 231, 272, 511, 981, 1091, 1903, 2490, 2684, 2990
Hpy99I CGWCG 1 cut(s) 2932
HpyCH4III ACNGT 5 cut(s) 726, 1353, 2285, 3143, 3284
HpyCH4IV ACGT 8 cut(s) 10, 227, 466, 931, 1382, 2060, 2686, 2927
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 1037, 1270, 1279, 1700, 3111
HpySE526I ACGT 8 cut(s) 10, 227, 466, 931, 1382, 2060, 2686, 2927
Hsp92I GRCGYC 1 cut(s) 2927
HspAI GCGC 2 cut(s) 1031, 1373
Kzo9I GATC 6 cut(s) 861, 2107, 2194, 2511, 3060, 3195
LmnI GCTCC 6 cut(s) 434, 520, 949, 1822, 2443, 2998
Lsp1109I GCAGC 4 cut(s) 571, 1052, 2166, 2494
LweI GCATC 5 cut(s) 352, 996, 1623, 1645, 3282
MaeI CTAG 7 cut(s) 261, 267, 960, 1544, 1742, 2531, 2837
MaeII ACGT 8 cut(s) 10, 227, 466, 931, 1382, 2060, 2686, 2927
MaeIII GTNAC 4 cut(s) 457, 606, 1675, 1797
MalI GATC 6 cut(s) 863, 2109, 2196, 2513, 3062, 3197
MbiI CCGCTC 1 cut(s) 1590
MboI GATC 6 cut(s) 861, 2107, 2194, 2511, 3060, 3195
MfeI CAATTG 3 cut(s) 372, 1921, 1949
MflI RGATCY 2 cut(s) 3060, 3195
MhlI GDGCHC 7 cut(s) 244, 439, 525, 954, 1290, 1827, 1905
MlsI TGGCCA 1 cut(s) 2946
MluNI TGGCCA 1 cut(s) 2946
MlyI GAGTC 2 cut(s) 399, 739
MmeI TCCRAC 5 cut(s) 102, 373, 1849, 2125, 2148
Mox20I TGGCCA 1 cut(s) 2946
Mph1103I ATGCAT 4 cut(s) 568, 1011, 1516, 1638
MscI TGGCCA 1 cut(s) 2946
MslI CAYNNNNRTG 1 cut(s) 697
Msp20I TGGCCA 1 cut(s) 2946
MspA1I CMGCKG 1 cut(s) 1043
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 2 cut(s) 275, 3092
MunI CAATTG 3 cut(s) 372, 1921, 1949
Mva1269I GAATGC 4 cut(s) 566, 1536, 1919, 1993
MvnI CGCG 4 cut(s) 656, 1033, 1373, 3027
MwoI GCNNNNNNNGC 6 cut(s) 42, 1037, 1270, 1279, 1700, 3111
NdeI CATATG 1 cut(s) 1510
NdeII GATC 6 cut(s) 861, 2107, 2194, 2511, 3060, 3195
NlaIV GGNNCC 3 cut(s) 522, 662, 1248
NmeAIII GCCGAG 2 cut(s) 904, 2230
NmuCI GTSAC 2 cut(s) 457, 1675
NsiI ATGCAT 4 cut(s) 568, 1011, 1516, 1638
NspI RCATGY 4 cut(s) 26, 566, 1798, 3244
NspV TTCGAA 2 cut(s) 846, 2360
PaeI GCATGC 1 cut(s) 566
PciI ACATGT 1 cut(s) 1794
PctI GAATGC 4 cut(s) 566, 1536, 1919, 1993
PfeI GAWTC 4 cut(s) 1558, 1730, 2782, 2918
PflMI CCANNNNNTGG 1 cut(s) 2708
PkrI GCNGC 5 cut(s) 561, 655, 1042, 2181, 2484
PleI GAGTC 2 cut(s) 398, 738
PmaCI CACGTG 1 cut(s) 467
PmlI CACGTG 1 cut(s) 467
PpsI GAGTC 2 cut(s) 398, 738
Ppu21I YACGTR 2 cut(s) 467, 932
PpuMI RGGWCCY 1 cut(s) 660
PscI ACATGT 1 cut(s) 1794
PshAI GACNNNNGTC 1 cut(s) 1682
PshBI ATTAAT 2 cut(s) 1485, 2430
Psp124BI GAGCTC 2 cut(s) 954, 1827
Psp5II RGGWCCY 1 cut(s) 660
PspCI CACGTG 1 cut(s) 467
PspN4I GGNNCC 3 cut(s) 522, 662, 1248
PspPI GGNCC 5 cut(s) 281, 660, 1701, 1738, 3163
PspPPI RGGWCCY 1 cut(s) 660
PstI CTGCAG 2 cut(s) 1422, 1468
PsuI RGATCY 2 cut(s) 3060, 3195
PvuII CAGCTG 1 cut(s) 1043
RsaI GTAC 3 cut(s) 934, 1092, 2897
RsaNI GTAC 3 cut(s) 933, 1091, 2896
RseI CAYNNNNRTG 1 cut(s) 697
SacI GAGCTC 2 cut(s) 954, 1827
SatI GCNGC 5 cut(s) 560, 654, 1041, 2180, 2483
Sau3AI GATC 6 cut(s) 861, 2107, 2194, 2511, 3060, 3195
Sau96I GGNCC 5 cut(s) 281, 660, 1701, 1738, 3163
SbfI CCTGCAGG 1 cut(s) 1422
ScaI AGTACT 1 cut(s) 2897
SchI GAGTC 2 cut(s) 399, 739
SdaI CCTGCAGG 1 cut(s) 1422
SduI GDGCHC 7 cut(s) 244, 439, 525, 954, 1290, 1827, 1905
SfaNI GCATC 5 cut(s) 352, 996, 1623, 1645, 3282
SfcI CTRYAG 2 cut(s) 1418, 1464
SfuI TTCGAA 2 cut(s) 846, 2360
SinI GGWCC 3 cut(s) 281, 660, 1738
SmiMI CAYNNNNRTG 1 cut(s) 697
SmlI CTYRAG 4 cut(s) 611, 1660, 2891, 3260
SmoI CTYRAG 4 cut(s) 611, 1660, 2891, 3260
SnaBI TACGTA 1 cut(s) 932
SpeI ACTAGT 1 cut(s) 2836
SphI GCATGC 1 cut(s) 566
Sse8387I CCTGCAGG 1 cut(s) 1422
SsiI CCGC 6 cut(s) 654, 1282, 1590, 1991, 2042, 3025
SspI AATATT 3 cut(s) 711, 2571, 2905
SspMI CTAG 7 cut(s) 261, 267, 960, 1544, 1742, 2531, 2837
SstI GAGCTC 2 cut(s) 954, 1827
StyI CCWWGG 2 cut(s) 2409, 2971
TaaI ACNGT 5 cut(s) 726, 1353, 2285, 3143, 3284
TaiI ACGT 8 cut(s) 13, 230, 469, 934, 1385, 2063, 2689, 2930
TatI WGTACW 2 cut(s) 1090, 2895
TauI GCSGC 1 cut(s) 656
TfiI GAWTC 4 cut(s) 1558, 1730, 2782, 2918
TscAI CASTG 1 cut(s) 3183
TseFI GTSAC 2 cut(s) 457, 1675
TseI GCWGC 4 cut(s) 559, 1040, 2179, 2482
Tsp45I GTSAC 2 cut(s) 457, 1675
TspDTI ATGAA 6 cut(s) 155, 855, 1848, 2000, 2087, 2445
TspRI CASTG 1 cut(s) 3183
Van91I CCANNNNNTGG 1 cut(s) 2708
Vha464I CTTAAG 1 cut(s) 611
VneI GTGCAC 1 cut(s) 1901
VpaK11BI GGWCC 3 cut(s) 281, 660, 1738
VspI ATTAAT 2 cut(s) 1485, 2430
XapI RAATTY 5 cut(s) 574, 842, 1777, 1853, 2214
XceI RCATGY 4 cut(s) 26, 566, 1798, 3244
XmiI GTMKAC 1 cut(s) 271
XspI CTAG 7 cut(s) 261, 267, 960, 1544, 1742, 2531, 2837
ZraI GACGTC 1 cut(s) 2928
ZrmI AGTACT 1 cut(s) 2897
Zsp2I ATGCAT 4 cut(s) 568, 1011, 1516, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.