disease resistance
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 67 |
| AatII | GACGTC | 1 cut(s) | 2366 |
| Acc36I | ACCTGC | 1 cut(s) | 67 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1134 |
| AccI | GTMKAC | 1 cut(s) | 3856 |
| AccII | CGCG | 3 cut(s) | 473, 843, 1379 |
| AciI | CCGC | 7 cut(s) | 408, 841, 1377, 1955, 2150, 2398, 3115 |
| AclI | AACGTT | 1 cut(s) | 1090 |
| AclWI | GGATC | 2 cut(s) | 2028, 3002 |
| AcoI | YGGCCR | 2 cut(s) | 2413, 3605 |
| AcsI | RAATTY | 5 cut(s) | 749, 1766, 1965, 2510, 2696 |
| AcuI | CTGAAG | 2 cut(s) | 2797, 3296 |
| AcyI | GRCGYC | 2 cut(s) | 345, 2363 |
| AdeI | CACNNNGTG | 1 cut(s) | 2933 |
| AfaI | GTAC | 5 cut(s) | 669, 771, 1139, 1754, 3213 |
| AfeI | AGCGCT | 1 cut(s) | 1318 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 381, 1134, 1749, 2047, 3368, 3443, 3581 |
| AflIII | ACRYGT | 2 cut(s) | 471, 3611 |
| AhlI | ACTAGT | 1 cut(s) | 2405 |
| AjnI | CCWGG | 5 cut(s) | 1127, 1607, 2046, 3436, 3743 |
| AleI | CACNNNNGTG | 1 cut(s) | 2028 |
| Alw21I | GWGCWC | 4 cut(s) | 117, 768, 1203, 1740 |
| Alw26I | GTCTC | 6 cut(s) | 1668, 1668, 2289, 2580, 2950, 3385 |
| AlwI | GGATC | 2 cut(s) | 2028, 3002 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 82 |
| Ama87I | CYCGRG | 3 cut(s) | 1970, 2953, 3491 |
| Aor51HI | AGCGCT | 1 cut(s) | 1318 |
| AoxI | GGCC | 7 cut(s) | 838, 1538, 1614, 2413, 3572, 3605, 3746 |
| ApaI | GGGCCC | 1 cut(s) | 1542 |
| ApoI | RAATTY | 5 cut(s) | 749, 1766, 1965, 2510, 2696 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 734, 766 |
| AseI | ATTAAT | 4 cut(s) | 927, 2343, 3510, 3525 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1447, 2844 |
| AspLEI | GCGC | 3 cut(s) | 1319, 1407, 1818 |
| AspS9I | GGNCC | 9 cut(s) | 578, 838, 1538, 1539, 1614, 1651, 1919, 3474, 3689 |
| AsuHPI | GGTGA | 7 cut(s) | 40, 998, 1259, 1732, 2290, 3136, 3301 |
| AsuII | TTCGAA | 2 cut(s) | 520, 1012 |
| AsuNHI | GCTAGC | 1 cut(s) | 3349 |
| AvaI | CYCGRG | 3 cut(s) | 1970, 2953, 3491 |
| AvaII | GGWCC | 5 cut(s) | 578, 1651, 1919, 3474, 3689 |
| BaeGI | GKGCMC | 1 cut(s) | 1542 |
| BanII | GRGCYC | 5 cut(s) | 117, 768, 1021, 1542, 1740 |
| BbsI | GAAGAC | 1 cut(s) | 2436 |
| Bbv12I | GWGCWC | 4 cut(s) | 117, 768, 1203, 1740 |
| BbvCI | CCTCAGC | 1 cut(s) | 2900 |
| BceAI | ACGGC | 1 cut(s) | 2400 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1406, 1440, 1442, 1476 |
| BciT130I | CCWGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| BclI | TGATCA | 1 cut(s) | 1513 |
| BcoDI | GTCTC | 6 cut(s) | 1668, 1668, 2289, 2580, 2950, 3385 |
| BcuI | ACTAGT | 1 cut(s) | 2405 |
| BfaI | CTAG | 8 cut(s) | 641, 1457, 1655, 2406, 2444, 2612, 3350, 3386 |
| BfoI | RGCGCY | 1 cut(s) | 1320 |
| BfuAI | ACCTGC | 1 cut(s) | 67 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1613 |
| Bme1390I | CCNGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| Bme18I | GGWCC | 5 cut(s) | 578, 1651, 1919, 3474, 3689 |
| BmeT110I | CYCGRG | 3 cut(s) | 1970, 2953, 3491 |
| BmgT120I | GGNCC | 9 cut(s) | 578, 838, 1538, 1539, 1614, 1651, 1919, 3474, 3689 |
| BmiI | GGNNCC | 4 cut(s) | 406, 1540, 1541, 3691 |
| BmrFI | CCNGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| BmrI | ACTGGG | 1 cut(s) | 2795 |
| BmsI | GCATC | 9 cut(s) | 85, 1436, 1558, 1594, 2212, 3213, 3362, 3419, 3789 |
| BmtI | GCTAGC | 1 cut(s) | 3353 |
| BmuI | ACTGGG | 1 cut(s) | 2795 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1595 |
| BpiI | GAAGAC | 1 cut(s) | 2436 |
| BpmI | CTGGAG | 2 cut(s) | 432, 2537 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2226, 2900 |
| Bpu14I | TTCGAA | 2 cut(s) | 520, 1012 |
| BpuEI | CTTGAG | 2 cut(s) | 101, 1558 |
| Bsa29I | ATCGAT | 1 cut(s) | 2997 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2999 |
| BsaHI | GRCGYC | 2 cut(s) | 345, 2363 |
| BsaI | GGTCTC | 1 cut(s) | 1668 |
| BsaJI | CCNNGG | 2 cut(s) | 1128, 1555 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 2267, 2297, 3110, 3116, 3140, 3146 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 381, 1134, 1749, 2047, 3368, 3443, 3581 |
| Bse118I | RCCGGY | 1 cut(s) | 1757 |
| Bse1I | ACTGG | 5 cut(s) | 500, 1296, 2022, 2790, 3413 |
| Bse3DI | GCAATG | 3 cut(s) | 1035, 1370, 3110 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2999 |
| BseBI | CCWGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| BseCI | ATCGAT | 1 cut(s) | 2997 |
| BseDI | CCNNGG | 2 cut(s) | 1128, 1555 |
| BseGI | GGATG | 8 cut(s) | 100, 188, 786, 1139, 1609, 2354, 2949, 3353 |
| BseJI | GATNNNNATC | 1 cut(s) | 2999 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 381, 1134, 1749, 2047, 3368, 3443, 3581 |
| BseMI | GCAATG | 3 cut(s) | 1035, 1370, 3110 |
| BseMII | CTCAG | 7 cut(s) | 1434, 1870, 1914, 2731, 2914, 2978, 3275 |
| BseNI | ACTGG | 5 cut(s) | 500, 1296, 2022, 2790, 3413 |
| BseRI | GAGGAG | 4 cut(s) | 224, 624, 2783, 3380 |
| BseSI | GKGCMC | 1 cut(s) | 1542 |
| BseYI | CCCAGC | 1 cut(s) | 3728 |
| Bsh1236I | CGCG | 3 cut(s) | 473, 843, 1379 |
| Bsh1285I | CGRYCG | 1 cut(s) | 3036 |
| BshFI | GGCC | 7 cut(s) | 840, 1540, 1616, 2415, 3574, 3607, 3748 |
| BshVI | ATCGAT | 1 cut(s) | 2997 |
| BsiEI | CGRYCG | 1 cut(s) | 3036 |
| BsiHKAI | GWGCWC | 4 cut(s) | 117, 768, 1203, 1740 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1970, 2953, 3491 |
| BsiSI | CCGG | 1 cut(s) | 1758 |
| BslFI | GGGAC | 3 cut(s) | 302, 3342, 3675 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 381, 1134, 1749, 2047, 3368, 3443, 3581 |
| BsmAI | GTCTC | 6 cut(s) | 1668, 1668, 2289, 2580, 2950, 3385 |
| BsmBI | CGTCTC | 1 cut(s) | 2580 |
| BsmFI | GGGAC | 3 cut(s) | 302, 3342, 3675 |
| BsmI | GAATGC | 2 cut(s) | 1449, 1906 |
| BsnI | GGCC | 7 cut(s) | 840, 1540, 1616, 2415, 3574, 3607, 3748 |
| Bso31I | GGTCTC | 1 cut(s) | 1668 |
| BsoBI | CYCGRG | 3 cut(s) | 1970, 2953, 3491 |
| Bsp119I | TTCGAA | 2 cut(s) | 520, 1012 |
| Bsp120I | GGGCCC | 1 cut(s) | 1538 |
| Bsp1286I | GDGCHC | 6 cut(s) | 117, 768, 1021, 1203, 1542, 1740 |
| Bsp143I | GATC | 9 cut(s) | 304, 313, 1513, 1728, 2020, 2994, 3033, 3178, 3760 |
| BspACI | CCGC | 7 cut(s) | 408, 841, 1377, 1955, 2150, 2398, 3115 |
| BspANI | GGCC | 7 cut(s) | 840, 1540, 1616, 2415, 3574, 3607, 3748 |
| BspCNI | CTCAG | 7 cut(s) | 1433, 1869, 1915, 2730, 2913, 2979, 3276 |
| BspDI | ATCGAT | 1 cut(s) | 2997 |
| BspFNI | CGCG | 3 cut(s) | 473, 843, 1379 |
| BspHI | TCATGA | 1 cut(s) | 3003 |
| BspLI | GGNNCC | 4 cut(s) | 406, 1540, 1541, 3691 |
| BspMI | ACCTGC | 1 cut(s) | 67 |
| BspOI | GCTAGC | 1 cut(s) | 3353 |
| BspPI | GGATC | 2 cut(s) | 2028, 3002 |
| BspQI | GCTCTTC | 1 cut(s) | 597 |
| BspT104I | TTCGAA | 2 cut(s) | 520, 1012 |
| BspTNI | GGTCTC | 1 cut(s) | 1668 |
| BsrDI | GCAATG | 3 cut(s) | 1035, 1370, 3110 |
| BsrFI | RCCGGY | 1 cut(s) | 1757 |
| BsrI | ACTGG | 5 cut(s) | 500, 1296, 2022, 2790, 3413 |
| BssAI | RCCGGY | 1 cut(s) | 1757 |
| BssECI | CCNNGG | 2 cut(s) | 1128, 1555 |
| BssMI | GATC | 9 cut(s) | 304, 313, 1513, 1728, 2020, 2994, 3033, 3178, 3760 |
| BssNAI | GTATAC | 1 cut(s) | 3857 |
| BssNI | GRCGYC | 2 cut(s) | 345, 2363 |
| Bst1107I | GTATAC | 1 cut(s) | 3857 |
| Bst2UI | CCWGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| Bst4CI | ACNGT | 7 cut(s) | 1182, 1266, 1699, 2198, 3044, 3190, 3396 |
| Bst6I | CTCTTC | 4 cut(s) | 15, 597, 701, 2583 |
| BstACI | GRCGYC | 2 cut(s) | 345, 2363 |
| BstBI | TTCGAA | 2 cut(s) | 520, 1012 |
| BstC8I | GCNNGC | 8 cut(s) | 74, 952, 1021, 1377, 1501, 1759, 2504, 3351 |
| BstF5I | GGATG | 8 cut(s) | 100, 188, 786, 1139, 1609, 2354, 2949, 3353 |
| BstFNI | CGCG | 3 cut(s) | 473, 843, 1379 |
| BstH2I | RGCGCY | 1 cut(s) | 1320 |
| BstHHI | GCGC | 3 cut(s) | 1319, 1407, 1818 |
| BstKTI | GATC | 9 cut(s) | 307, 316, 1516, 1731, 2023, 2997, 3036, 3181, 3763 |
| BstMAI | GTCTC | 6 cut(s) | 1668, 1668, 2289, 2580, 2950, 3385 |
| BstMBI | GATC | 9 cut(s) | 304, 313, 1513, 1728, 2020, 2994, 3033, 3178, 3760 |
| BstMCI | CGRYCG | 1 cut(s) | 3036 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 944, 1546, 1613, 2101, 2485, 2901, 3294, 3682 |
| BstNI | CCWGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| BstNSI | RCATGY | 2 cut(s) | 76, 1711 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1595 |
| BstSCI | CCNGG | 5 cut(s) | 1127, 1607, 2046, 3436, 3743 |
| BstSLI | GKGCMC | 1 cut(s) | 1542 |
| BstUI | CGCG | 3 cut(s) | 473, 843, 1379 |
| BstV2I | GAAGAC | 1 cut(s) | 2436 |
| BstZ17I | GTATAC | 1 cut(s) | 3857 |
| Bsu15I | ATCGAT | 1 cut(s) | 2997 |
| BsuRI | GGCC | 7 cut(s) | 840, 1540, 1616, 2415, 3574, 3607, 3748 |
| BsuTUI | ATCGAT | 1 cut(s) | 2997 |
| BtsCI | GGATG | 8 cut(s) | 100, 188, 786, 1139, 1609, 2354, 2949, 3353 |
| BtsI | GCAGTG | 3 cut(s) | 1481, 1911, 2830 |
| BtsIMutI | CAGTG | 5 cut(s) | 1481, 1695, 1911, 2830, 3420 |
| BveI | ACCTGC | 1 cut(s) | 67 |
| Cac8I | GCNNGC | 8 cut(s) | 74, 952, 1021, 1377, 1501, 1759, 2504, 3351 |
| CaiI | CAGNNNCTG | 1 cut(s) | 82 |
| CciI | TCATGA | 1 cut(s) | 3003 |
| CfoI | GCGC | 3 cut(s) | 1319, 1407, 1818 |
| Cfr10I | RCCGGY | 1 cut(s) | 1757 |
| Cfr13I | GGNCC | 9 cut(s) | 578, 838, 1538, 1539, 1614, 1651, 1919, 3474, 3689 |
| ClaI | ATCGAT | 1 cut(s) | 2997 |
| CseI | GACGC | 1 cut(s) | 334 |
| Csp6I | GTAC | 5 cut(s) | 668, 770, 1138, 1753, 3212 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1684, 1719 |
| CviQI | GTAC | 5 cut(s) | 668, 770, 1138, 1753, 3212 |
| DpnI | GATC | 9 cut(s) | 306, 315, 1515, 1730, 2022, 2996, 3035, 3180, 3762 |
| DpnII | GATC | 9 cut(s) | 304, 313, 1513, 1728, 2020, 2994, 3033, 3178, 3760 |
| DraIII | CACNNNGTG | 1 cut(s) | 2933 |
| EaeI | YGGCCR | 2 cut(s) | 2413, 3605 |
| Eam1104I | CTCTTC | 4 cut(s) | 15, 597, 701, 2583 |
| EarI | CTCTTC | 4 cut(s) | 15, 597, 701, 2583 |
| Ecl136II | GAGCTC | 3 cut(s) | 115, 766, 1738 |
| Eco147I | AGGCCT | 1 cut(s) | 3574 |
| Eco24I | GRGCYC | 5 cut(s) | 117, 768, 1021, 1542, 1740 |
| Eco31I | GGTCTC | 1 cut(s) | 1668 |
| Eco32I | GATATC | 2 cut(s) | 283, 3001 |
| Eco47I | GGWCC | 5 cut(s) | 578, 1651, 1919, 3474, 3689 |
| Eco47III | AGCGCT | 1 cut(s) | 1318 |
| Eco53kI | GAGCTC | 3 cut(s) | 115, 766, 1738 |
| Eco57I | CTGAAG | 2 cut(s) | 2797, 3296 |
| Eco88I | CYCGRG | 3 cut(s) | 1970, 2953, 3491 |
| EcoICRI | GAGCTC | 3 cut(s) | 115, 766, 1738 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1538, 1539 |
| EcoRII | CCWGG | 5 cut(s) | 1127, 1607, 2046, 3436, 3743 |
| EcoRV | GATATC | 2 cut(s) | 283, 3001 |
| EcoT22I | ATGCAT | 1 cut(s) | 918 |
| EcoT38I | GRGCYC | 5 cut(s) | 117, 768, 1021, 1542, 1740 |
| Esp3I | CGTCTC | 1 cut(s) | 2580 |
| FaqI | GGGAC | 3 cut(s) | 302, 3342, 3675 |
| FauI | CCCGC | 2 cut(s) | 1384, 3108 |
| FbaI | TGATCA | 1 cut(s) | 1513 |
| FblI | GTMKAC | 1 cut(s) | 3856 |
| FokI | GGATG | 8 cut(s) | 107, 175, 793, 1146, 1616, 2361, 2936, 3340 |
| FriOI | GRGCYC | 5 cut(s) | 117, 768, 1021, 1542, 1740 |
| FspBI | CTAG | 8 cut(s) | 641, 1457, 1655, 2406, 2444, 2612, 3350, 3386 |
| GlaI | GCGC | 3 cut(s) | 1318, 1406, 1817 |
| GsaI | CCCAGC | 1 cut(s) | 3732 |
| GsuI | CTGGAG | 2 cut(s) | 432, 2537 |
| HaeII | RGCGCY | 1 cut(s) | 1320 |
| HaeIII | GGCC | 7 cut(s) | 840, 1540, 1616, 2415, 3574, 3607, 3748 |
| HapII | CCGG | 1 cut(s) | 1758 |
| HgaI | GACGC | 1 cut(s) | 334 |
| HhaI | GCGC | 3 cut(s) | 1319, 1407, 1818 |
| Hin1I | GRCGYC | 2 cut(s) | 345, 2363 |
| Hin6I | GCGC | 3 cut(s) | 1317, 1405, 1816 |
| HinP1I | GCGC | 3 cut(s) | 1317, 1405, 1816 |
| HincII | GTYRAC | 1 cut(s) | 2403 |
| HindII | GTYRAC | 1 cut(s) | 2403 |
| HindIII | AAGCTT | 2 cut(s) | 7, 2504 |
| HpaII | CCGG | 1 cut(s) | 1758 |
| HphI | GGTGA | 7 cut(s) | 40, 998, 1259, 1732, 2290, 3136, 3301 |
| Hpy166II | GTNNAC | 5 cut(s) | 1094, 2403, 2780, 3668, 3857 |
| Hpy8I | GTNNAC | 5 cut(s) | 1094, 2403, 2780, 3668, 3857 |
| Hpy99I | CGWCG | 2 cut(s) | 350, 591 |
| HpyCH4III | ACNGT | 7 cut(s) | 1182, 1266, 1699, 2198, 3044, 3190, 3396 |
| HpyCH4IV | ACGT | 5 cut(s) | 586, 1090, 2363, 3456, 3611 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 944, 1546, 1613, 2101, 2485, 2901, 3294, 3682 |
| HpySE526I | ACGT | 5 cut(s) | 586, 1090, 2363, 3456, 3611 |
| Hsp92I | GRCGYC | 2 cut(s) | 345, 2363 |
| HspAI | GCGC | 3 cut(s) | 1317, 1405, 1816 |
| KroI | GCCGGC | 1 cut(s) | 1757 |
| KroNI | GCCGGC | 1 cut(s) | 1759 |
| Ksp22I | TGATCA | 1 cut(s) | 1513 |
| Kzo9I | GATC | 9 cut(s) | 304, 313, 1513, 1728, 2020, 2994, 3033, 3178, 3760 |
| LguI | GCTCTTC | 1 cut(s) | 597 |
| LmnI | GCTCC | 4 cut(s) | 350, 404, 763, 1735 |
| LweI | GCATC | 9 cut(s) | 85, 1436, 1558, 1594, 2212, 3213, 3362, 3419, 3789 |
| MaeI | CTAG | 8 cut(s) | 641, 1457, 1655, 2406, 2444, 2612, 3350, 3386 |
| MaeII | ACGT | 5 cut(s) | 586, 1090, 2363, 3456, 3611 |
| MalI | GATC | 9 cut(s) | 306, 315, 1515, 1730, 2022, 2996, 3035, 3180, 3762 |
| MboI | GATC | 9 cut(s) | 304, 313, 1513, 1728, 2020, 2994, 3033, 3178, 3760 |
| MfeI | CAATTG | 5 cut(s) | 1834, 1862, 2483, 3579, 3671 |
| MhlI | GDGCHC | 6 cut(s) | 117, 768, 1021, 1203, 1542, 1740 |
| MluI | ACGCGT | 1 cut(s) | 471 |
| MlyI | GAGTC | 5 cut(s) | 661, 1412, 2258, 3135, 3565 |
| MmeI | TCCRAC | 8 cut(s) | 328, 514, 1416, 1762, 2038, 2061, 3006, 3102 |
| Mph1103I | ATGCAT | 1 cut(s) | 918 |
| MroNI | GCCGGC | 1 cut(s) | 1757 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1447, 2844 |
| MslI | CAYNNNNRTG | 3 cut(s) | 77, 2028, 2220 |
| MspA1I | CMGCKG | 4 cut(s) | 410, 1957, 2398, 2569 |
| MspI | CCGG | 1 cut(s) | 1758 |
| MspR9I | CCNGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| MunI | CAATTG | 5 cut(s) | 1834, 1862, 2483, 3579, 3671 |
| Mva1269I | GAATGC | 2 cut(s) | 1449, 1906 |
| MvaI | CCWGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| MvnI | CGCG | 3 cut(s) | 473, 843, 1379 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 944, 1546, 1613, 2101, 2485, 2901, 3294, 3682 |
| NaeI | GCCGGC | 1 cut(s) | 1759 |
| NdeII | GATC | 9 cut(s) | 304, 313, 1513, 1728, 2020, 2994, 3033, 3178, 3760 |
| NgoMIV | GCCGGC | 1 cut(s) | 1757 |
| NheI | GCTAGC | 1 cut(s) | 3349 |
| NlaIV | GGNNCC | 4 cut(s) | 406, 1540, 1541, 3691 |
| NmeAIII | GCCGAG | 1 cut(s) | 3583 |
| NmuCI | GTSAC | 5 cut(s) | 28, 160, 377, 1588, 3028 |
| NsiI | ATGCAT | 1 cut(s) | 918 |
| NspI | RCATGY | 2 cut(s) | 76, 1711 |
| NspV | TTCGAA | 2 cut(s) | 520, 1012 |
| OliI | CACNNNNGTG | 1 cut(s) | 2028 |
| PaeI | GCATGC | 1 cut(s) | 76 |
| PaeR7I | CTCGAG | 2 cut(s) | 1970, 3491 |
| PagI | TCATGA | 1 cut(s) | 3003 |
| PaqCI | CACCTGC | 1 cut(s) | 67 |
| PceI | AGGCCT | 1 cut(s) | 3574 |
| PciSI | GCTCTTC | 1 cut(s) | 597 |
| PctI | GAATGC | 2 cut(s) | 1449, 1906 |
| PdiI | GCCGGC | 1 cut(s) | 1759 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1447, 2844 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1134 |
| Ple19I | CGATCG | 1 cut(s) | 3036 |
| PleI | GAGTC | 5 cut(s) | 660, 1412, 2257, 3135, 3564 |
| PpsI | GAGTC | 5 cut(s) | 660, 1412, 2257, 3135, 3564 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1595 |
| PshBI | ATTAAT | 4 cut(s) | 927, 2343, 3510, 3525 |
| Psp124BI | GAGCTC | 3 cut(s) | 117, 768, 1740 |
| Psp1406I | AACGTT | 1 cut(s) | 1090 |
| Psp6I | CCWGG | 5 cut(s) | 1127, 1607, 2046, 3436, 3743 |
| PspFI | CCCAGC | 1 cut(s) | 3728 |
| PspGI | CCWGG | 5 cut(s) | 1127, 1607, 2046, 3436, 3743 |
| PspN4I | GGNNCC | 4 cut(s) | 406, 1540, 1541, 3691 |
| PspOMI | GGGCCC | 1 cut(s) | 1538 |
| PspPI | GGNCC | 9 cut(s) | 578, 838, 1538, 1539, 1614, 1651, 1919, 3474, 3689 |
| PstNI | CAGNNNCTG | 1 cut(s) | 82 |
| PvuI | CGATCG | 1 cut(s) | 3036 |
| PvuII | CAGCTG | 1 cut(s) | 2569 |
| RsaI | GTAC | 5 cut(s) | 669, 771, 1139, 1754, 3213 |
| RsaNI | GTAC | 5 cut(s) | 668, 770, 1138, 1753, 3212 |
| RseI | CAYNNNNRTG | 3 cut(s) | 77, 2028, 2220 |
| SacI | GAGCTC | 3 cut(s) | 117, 768, 1740 |
| SapI | GCTCTTC | 1 cut(s) | 597 |
| Sau3AI | GATC | 9 cut(s) | 304, 313, 1513, 1728, 2020, 2994, 3033, 3178, 3760 |
| Sau96I | GGNCC | 9 cut(s) | 578, 838, 1538, 1539, 1614, 1651, 1919, 3474, 3689 |
| SchI | GAGTC | 5 cut(s) | 661, 1412, 2258, 3135, 3565 |
| ScrFI | CCNGG | 5 cut(s) | 1129, 1609, 2048, 3438, 3745 |
| SduI | GDGCHC | 6 cut(s) | 117, 768, 1021, 1203, 1542, 1740 |
| SfaNI | GCATC | 9 cut(s) | 85, 1436, 1558, 1594, 2212, 3213, 3362, 3419, 3789 |
| Sfr274I | CTCGAG | 2 cut(s) | 1970, 3491 |
| SfuI | TTCGAA | 2 cut(s) | 520, 1012 |
| SinI | GGWCC | 5 cut(s) | 578, 1651, 1919, 3474, 3689 |
| SlaI | CTCGAG | 2 cut(s) | 1970, 3491 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 77, 2028, 2220 |
| SmlI | CTYRAG | 4 cut(s) | 116, 1573, 1970, 3491 |
| SmoI | CTYRAG | 4 cut(s) | 116, 1573, 1970, 3491 |
| SpeI | ACTAGT | 1 cut(s) | 2405 |
| SphI | GCATGC | 1 cut(s) | 76 |
| SseBI | AGGCCT | 1 cut(s) | 3574 |
| SsiI | CCGC | 7 cut(s) | 408, 841, 1377, 1955, 2150, 2398, 3115 |
| SspI | AATATT | 7 cut(s) | 459, 618, 1145, 2493, 3323, 3508, 3514 |
| SspMI | CTAG | 8 cut(s) | 641, 1457, 1655, 2406, 2444, 2612, 3350, 3386 |
| SstI | GAGCTC | 3 cut(s) | 117, 768, 1740 |
| StuI | AGGCCT | 1 cut(s) | 3574 |
| StyD4I | CCNGG | 5 cut(s) | 1127, 1607, 2046, 3436, 3743 |
| TaaI | ACNGT | 7 cut(s) | 1182, 1266, 1699, 2198, 3044, 3190, 3396 |
| TaiI | ACGT | 5 cut(s) | 589, 1093, 2366, 3459, 3614 |
| TatI | WGTACW | 1 cut(s) | 667 |
| TauI | GCSGC | 2 cut(s) | 410, 843 |
| TscAI | CASTG | 5 cut(s) | 1488, 1702, 1911, 2837, 3420 |
| TseFI | GTSAC | 5 cut(s) | 28, 160, 377, 1588, 3028 |
| Tsp45I | GTSAC | 5 cut(s) | 28, 160, 377, 1588, 3028 |
| TspGWI | ACGGA | 6 cut(s) | 507, 1703, 2040, 2820, 3229, 3466 |
| TspRI | CASTG | 5 cut(s) | 1488, 1702, 1911, 2837, 3420 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1134 |
| VpaK11BI | GGWCC | 5 cut(s) | 578, 1651, 1919, 3474, 3689 |
| VspI | ATTAAT | 4 cut(s) | 927, 2343, 3510, 3525 |
| XapI | RAATTY | 5 cut(s) | 749, 1766, 1965, 2510, 2696 |
| XceI | RCATGY | 2 cut(s) | 76, 1711 |
| XhoI | CTCGAG | 2 cut(s) | 1970, 3491 |
| XmiI | GTMKAC | 1 cut(s) | 3856 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1447, 2844 |
| XspI | CTAG | 8 cut(s) | 641, 1457, 1655, 2406, 2444, 2612, 3350, 3386 |
| ZraI | GACGTC | 1 cut(s) | 2364 |
| Zsp2I | ATGCAT | 1 cut(s) | 918 |