MD11G1045100.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
3943874 .. 3948213
4340 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1045100.v1.1.491

Sequence Viewer

Length: 4101 bp
ATGTCACTAGGAGAGGTCTTTATTTCTGGATTACTCCAAGTGTTACTTGAAAGGTTGACATCTCGTGAGACGCTGAGCTACTTTAGACGCCTACTTGGCGTTGGCAAAGAGCTAGACAAATGGAATTCAATGTTGTTTGCTATTCAAGCGGTACTGAATGACGCAGAGGAGAAGCAGTTGACAAGCCGAGCAATGAAATTGTGGCTGGATGGTCTTAGAGACTTGGCTTATGATGTGGAGGACATATTGGACAAGTATTCAACCGAGATATTGCGACGCCAAATAAAGAAGAAACATTGGGCTACCACAAGCAAAGTATGGGGATTGTATTCTGATGTTAAATTTAACTATAACTTGAGCTCAGAAATAAAGAAGATTACTGATCGGTTACATGAGATAATGGAACGGAAAAACCAACTTGGTTTAAATGAGGGGACGCCATCTACTAAGGAATGGCATGTGTCACCAAGTTCCTATCTACCAGAAGGACCCGTGATTGGAAGGGATACAGACAAGACGAAGATTATTGACTTGTTGTTGAAAGAAGCACCCCGTGTCGTCAACTTTCATGTAGTTGCCATTGTCGGTATGCCTGGAGTGGGAAAGACAACACTTGCAAGGCATGTTTATGATGATGACGCGACAAAAGAGTTTAGCCTAAAAGTATGGGTATCCGTGTCGGATGACTTCAATCTTGAAAGAGTGACAAAGGCAATTCTTGAATCTGCCACATCTGGTCCAATGCCGGAATTCAAGGAATTCAACAAGGTTCAAGAAAGTTTGAGTAAGGAGCTAGCAGGAAAAAAGTTTCTAATTGTTTTAGACGATGTTTGGAATACATGTGTCTATGATTTATGGATAAAATTGCAGTCCCCCTTTCGTGTTGGAGCATTAGGAAGTAAGATAGTTGTGACAACACGTGATGCAAAGGTTGCAAACATGATGGGATCCACTGTTTATCAATTGGATTGCATGACAGATGATCAATGTTGGGAAGTCTTTGAGCAACATTGCCTTTTGGACTTTACCAAAAGGCCGCAAAATTTTAAGTTGGTTAAGGAGAAAATTGTTGCAAAATGTCGCGGATTGCCATTGGCTGCAAGGACTCTTGGAGGTCTTCTACGTTGTAAACAAGTTGAAGCATGGGAGGAAATCTTAAACAACAAGATGTGGAGTTTATCAGACGATAGTGGCATTCTCCCGGTATTAAAATTGAGCTATCACTATCTCCCTTCGAGTTTGAAACGGTGCTTCGCCTATTGTTCCCTACTTCCGAATGACTATGAGTTCGGGGAGAAGCAGTTGATCCTTCTGTGGATGGCAGAAGGTTTGATTCAACAAAGAGTAGAGGACAAGAAACAACTGGAAGAAGTAGGCAGTGACTATTTTCAAGAGCTATTGTCCAGGTCATTATTTCAAAAGGCAAGCAAAAGCAATGATAAGTATGTAATGCATGACCTTGTTGGTGAGTTGGCGCGGTGGGCTGCAGCTGAAATATGCTTTTCATTGGAGGATAAGGGAAATGATGACTTGCATCCAATGGCTCGTCATATGTCTTATATTATTGGTAAGTATGATGGGGTTAAAAAATTTGAGGCCATTTCAAGAGTTAAACATTTGAGGACATTCTTGCCGCTTCCACAATCAAGGTATAACTCTCTAACTCGTAGGGTTACATTTGATCTATTGCCAGAACTGCGAATCTTACGGGTGTTTTCTTTGAATGGCTATGAAATAACTGAGCTGCCCAATTCCATTGGTAATTTGTGGAATCTACGGTATCTGGACCTTTCTTATACAAAAATAAAGAGTTTGCCTCCATCAACAACCACTCTTTGCAATCTGCAAACATTGTTATTAGAAGGTTGTTCTGAATTGGAGGCATTGCCCGCAAACATGAGTAATCTAATTAATTTGCGGCATCTCAACAGTTCCAGTGGATTGGACAGAATGCCTCCGCATCTAGGTCGATTGACGAATCTGCAATCCTTGTCGTATTTTGTGGTAGGCAAAGGTAGTGATCAGTCTGGAATAAGAGAGATAGGGTCCCTACTGCATCTCCGAGGGACATTGTGCCTCTCAAGATTGGAGAATGTGGTTGATGCTAAGGATGCTAGCGGGGCCAGGTTAATAGACAAGGAGAGGCTTGATTCGTTGAAGCTGGAATGGTCTGATTTGAGCGGATCGAGGGAAATGGAGTTGGGTGTGCTTGACATGTTAAAACCTCCTAAAAATCTCAAAGAGCTCACCATTGAGGGTTATGCTGGATTGAAATTTTCATCGTGGATTGGAGATCCTCTGTTCTCTAACATGGTCCGTGTAAGCTTATACAATTGTGCAAGTTGTCATCTCTTGCCACCAGTTGGACAGTTGCCTTGCCTTAAAGAACTTTATATCAGGGGAATGACTGCCGTGGAAATCGTTGGTCCTGAATTCTACGGAGAGGGTAGCTTGCCTTTTCGAGTATTAGAGATTCTTCAGTTTTCGAGCATGCAACACTGGGAGAAATGGCTGCCTTTCGATCAGGATAAGGGAAGTGGTGTTTTCCCTAGCCTAAAAGTGCTTTCAATCAGAAAGTGCCCTAAACTGGAGGGCGAGTTGCCGAAGAAACTTAATTCGTTAGCAAAGCTTGAGATTGTTGGATGCAAGGAATTGGTGGTGGAAATTGCCAATTATGAACACGCGAGTGAAGTAAGCATCCATGGCTGCAAAGCGCTTGTGCATACTAGCGCAGAGGTTGACTTTAAGTTATTAGAGACCATGCGGCTTTCAAATATTTTGGAGCCGCAGTTGGGAGCAGGGGGACTTACGAAAGGATTGGGCAAGATTAAGGAGTTGAAGATTAGTGGTTGTGAGGAGCTGACGTCTTCACTGGAAAAAGAGGATAGATGTTTCCCACACCTGATTTCTCTTGTTCGTTTGGTTATTGAAGGCAACTCTGCCCTTGTTGAAGGTCTCGGAAAAGAATTGGAGGGGTTGCTGCAGGTTCCAATTTTAGCTTGTAAGCTTGAATATCTGAAAATAAACAAATGTGAACGTCTTTCGAAGCTGCCGAAGGGGTTGCATCAGTTGTCGTTTCTTCAAGAGCTTCACATAAATGACTGTGAAAGTCTAGTTTCTTTTCCAGATGTTGGCCTGCCACCTTCTCTTAAAGTCTTGGAGATTAAACACTGTAAGTCTCTGACGTATATTGCAAAATACCAGATTCCCCAAAATCTGAAAAGAATAGAGATATGGTTTTGTGGAAGTTTGAAATCATTAGTGGAGGAGGAGGAGGAGCTCGTTGTTTCTTGTTCATCTTCTTTTTCTGTTTCTCTTGAGCACTTGGAGGTATATTATTGTCCATCTCTGACATCGTTATCACTGAGAGGCCAGCTTTACAGGGCGCTTAAACACCTTCAAATAATCGAATGTGAAGGGCTACAGTTAATAGCATCAGACCGGTTCTTCCACGACAATACTAATAATTGTCTTGAATATATTAACATTGTCAGGTGTCCAAATCTGAAATCCTTACCGGAGGGCCTATGCCACCTCACCAATCTTCAAGCTTTTACTGTTAGGGGCTGCAGAAAATTGGAGGTGTCACCGGAAGACATTCGCAATCTCACGTCTCTTAAGGAATTGGAGATTGACTGCTTGGAAGGTTTGACTTCCTTTCCTCCCAACCTCACATCACTTGGAATTTATGAAGTCAAGAGTTGTAAGCGGCTCTTGGAGTTGGAGTGGGGGTTGCACAAACTCACGTCTCTCAAATCTTTGTCGATCGGAAGTAAAGATCCAGATGTGTTGTCATTTCCACCTGATGGGAAGGAGGAGATGCTGCTCCCCAAATCTCTCACTGCACTCACAATTAGTGGCTTCCCAAATCTGAAGAAACTCAACAATGGCATTCAATTCCTCACCTCTCTTCAGACTCTGCAACTAATTGATTGTCCAAAGCTCGCATCCATTCCAGACGAAGGTCTGCCTCTTTCACTTGGGGAACTTTCGATCGTCAAGTGTCCTTTACTGAAAGAGAGATGTCAACCTGGAAAAGGACGATACTGGCCCAAAATATCCCGCATTCCTTGCCTATATATAGATTATGAGACGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1367

Amino Acids

154.31

Weight (kDa)

6.76

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 99 2.5e-22 Rx N-terminal domain
NB-ARC PF00931 169 - 337 8.5e-34 NB-ARC domain
WHD_DRP PF23559 424 - 492 1.2e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 563 - 863 4.3e-26 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 678 - 802 6.5e-35 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2867
Acc36I ACCTGC 1 cut(s) 2974
AccB7I CCANNNNNTGG 3 cut(s) 2363, 3131, 3806
AccBSI CCGCTC 1 cut(s) 2181
AccII CGCG 4 cut(s) 641, 1083, 1477, 2684
AclWI GGATC 6 cut(s) 942, 955, 1300, 2191, 2288, 3773
AcsI RAATTY 9 cut(s) 124, 341, 749, 758, 1042, 1589, 2273, 2432, 3684
AcuI CTGAAG 3 cut(s) 2462, 3893, 3896
AcvI CACGTG 1 cut(s) 920
AcyI GRCGYC 4 cut(s) 88, 277, 437, 2864
AdeI CACNNNGTG 1 cut(s) 554
AfaI GTAC 1 cut(s) 153
AfeI AGCGCT 1 cut(s) 2715
AflII CTTAAG 1 cut(s) 3617
AflIII ACRYGT 3 cut(s) 839, 917, 2214
AgeI ACCGGT 1 cut(s) 3441
AjiI CACGTC 2 cut(s) 3612, 3747
AjnI CCWGG 4 cut(s) 592, 1403, 2123, 4030
AleI CACNNNNGTG 1 cut(s) 2685
Alw21I GWGCWC 4 cut(s) 362, 2247, 3282, 3324
Alw26I GTCTC 8 cut(s) 62, 213, 2750, 2960, 3183, 3618, 3753, 4085
AlwI GGATC 6 cut(s) 942, 955, 1300, 2191, 2288, 3773
AlwNI CAGNNNCTG 1 cut(s) 3919
Aor51HI AGCGCT 1 cut(s) 2715
AoxI GGCC 7 cut(s) 1034, 1596, 2121, 3133, 3370, 3523, 4049
ApoI RAATTY 9 cut(s) 124, 341, 749, 758, 1042, 1589, 2273, 2432, 3684
ArsI GACNNNNNNTTYG 2 cut(s) 1271, 1303
AseI ATTAAT 1 cut(s) 1911
AsiGI ACCGGT 1 cut(s) 3441
Asp700I GAANNNNTTC 1 cut(s) 3597
AspLEI GCGC 4 cut(s) 1477, 2716, 2732, 3388
AspS9I GGNCC 9 cut(s) 488, 737, 1786, 2046, 2121, 2314, 2426, 3523, 4050
AsuC2I CCSGG 1 cut(s) 1202
AsuHPI GGTGA 6 cut(s) 456, 1478, 2239, 3529, 3579, 3895
AsuII TTCGAA 1 cut(s) 3044
AsuNHI GCTAGC 2 cut(s) 793, 2114
AvaII GGWCC 6 cut(s) 488, 737, 1786, 2046, 2314, 2426
BaeGI GKGCMC 1 cut(s) 2582
BamHI GGATCC 1 cut(s) 947
BanII GRGCYC 3 cut(s) 362, 2247, 3282
BauI CACGAG 1 cut(s) 63
BbrPI CACGTG 1 cut(s) 920
BbsI GAAGAC 3 cut(s) 1109, 2859, 3600
Bbv12I GWGCWC 4 cut(s) 362, 2247, 3282, 3324
BccI CCATC 8 cut(s) 203, 448, 937, 1312, 1571, 1828, 3352, 3800
BceAI ACGGC 1 cut(s) 2396
BcgI CGANNNNNNTGC 4 cut(s) 1949, 1983, 3043, 3077
BciT130I CCWGG 4 cut(s) 594, 1405, 2125, 4032
BciVI GTATCC 2 cut(s) 499, 682
BclI TGATCA 2 cut(s) 982, 2020
BcnI CCSGG 1 cut(s) 1202
BcoDI GTCTC 8 cut(s) 62, 213, 2750, 2960, 3183, 3618, 3753, 4085
BfaI CTAG 8 cut(s) 8, 113, 794, 1964, 2115, 2550, 2727, 3113
BfmI CTRYAG 4 cut(s) 1485, 2981, 3422, 3568
BfoI RGCGCY 2 cut(s) 2717, 3389
BfrI CTTAAG 1 cut(s) 3617
BfuAI ACCTGC 1 cut(s) 2974
BfuI GTATCC 2 cut(s) 499, 682
BglI GCCNNNNNGGC 1 cut(s) 96
BlpI GCTNAGC 1 cut(s) 74
Bme1390I CCNGG 5 cut(s) 594, 1202, 1405, 2125, 4032
Bme18I GGWCC 6 cut(s) 488, 737, 1786, 2046, 2314, 2426
BmgBI CACGTC 2 cut(s) 3612, 3747
BmgT120I GGNCC 9 cut(s) 488, 737, 1786, 2046, 2121, 2314, 2426, 3523, 4050
BmiI GGNNCC 7 cut(s) 490, 949, 2047, 2048, 2122, 2784, 2988
BmrFI CCNGG 5 cut(s) 594, 1202, 1405, 2125, 4032
BmrI ACTGGG 1 cut(s) 2509
BmtI GCTAGC 2 cut(s) 797, 2118
BmuI ACTGGG 1 cut(s) 2509
BoxI GACNNNNGTC 1 cut(s) 3963
BpiI GAAGAC 3 cut(s) 1109, 2859, 3600
BplI GAGNNNNNCTC 2 cut(s) 1801, 1833
BpmI CTGGAG 2 cut(s) 615, 2609
Bpu10I CCTNAGC 1 cut(s) 2106
Bpu1102I GCTNAGC 1 cut(s) 74
Bpu14I TTCGAA 1 cut(s) 3044
BpuEI CTTGAG 4 cut(s) 376, 2065, 2651, 3338
BpuMI CCSGG 1 cut(s) 1202
BsaAI YACGTR 1 cut(s) 920
BsaHI GRCGYC 4 cut(s) 88, 277, 437, 2864
BsaI GGTCTC 2 cut(s) 2750, 2960
BsaJI CCNNGG 3 cut(s) 2062, 2412, 2701
BsaWI WCCGGW 3 cut(s) 3441, 3517, 3589
Bse118I RCCGGY 1 cut(s) 3441
Bse1I ACTGG 7 cut(s) 1368, 1935, 2360, 2504, 2592, 2877, 4052
Bse3DI GCAATG 4 cut(s) 198, 1009, 1441, 1883
BseBI CCWGG 4 cut(s) 594, 1405, 2125, 4032
BseDI CCNNGG 3 cut(s) 2062, 2412, 2701
BseGI GGATG 8 cut(s) 214, 688, 1323, 1534, 2116, 2648, 2697, 3947
BseMI GCAATG 4 cut(s) 198, 1009, 1441, 1883
BseMII CTCAG 4 cut(s) 65, 375, 1731, 3356
BseNI ACTGG 7 cut(s) 1368, 1935, 2360, 2504, 2592, 2877, 4052
BseRI GAGGAG 7 cut(s) 182, 2870, 3281, 3284, 3287, 3290, 3830
BseSI GKGCMC 1 cut(s) 2582
BsgI GTGCAG 1 cut(s) 3828
Bsh1236I CGCG 4 cut(s) 641, 1083, 1477, 2684
Bsh1285I CGRYCG 2 cut(s) 3768, 3996
BshFI GGCC 7 cut(s) 1036, 1598, 2123, 3135, 3372, 3525, 4051
BshTI ACCGGT 1 cut(s) 3441
BsiEI CGRYCG 2 cut(s) 3768, 3996
BsiHKAI GWGCWC 4 cut(s) 362, 2247, 3282, 3324
BsiSI CCGG 5 cut(s) 746, 1202, 3442, 3518, 3590
BslFI GGGAC 5 cut(s) 448, 856, 2032, 2080, 2817
BsmAI GTCTC 8 cut(s) 62, 213, 2750, 2960, 3183, 3618, 3753, 4085
BsmBI CGTCTC 4 cut(s) 62, 3618, 3753, 4085
BsmFI GGGAC 5 cut(s) 448, 856, 2032, 2080, 2817
BsmI GAATGC 4 cut(s) 1194, 1956, 3891, 4065
BsnI GGCC 7 cut(s) 1036, 1598, 2123, 3135, 3372, 3525, 4051
Bso31I GGTCTC 2 cut(s) 2750, 2960
Bsp119I TTCGAA 1 cut(s) 3044
Bsp1286I GDGCHC 5 cut(s) 362, 2247, 2582, 3282, 3324
Bsp1720I GCTNAGC 1 cut(s) 74
Bsp19I CCATGG 1 cut(s) 2701
BspANI GGCC 7 cut(s) 1036, 1598, 2123, 3135, 3372, 3525, 4051
BspCNI CTCAG 4 cut(s) 66, 374, 1732, 3357
BspFNI CGCG 4 cut(s) 641, 1083, 1477, 2684
BspLI GGNNCC 7 cut(s) 490, 949, 2047, 2048, 2122, 2784, 2988
BspMAI CTGCAG 3 cut(s) 1489, 2985, 3572
BspMI ACCTGC 1 cut(s) 2974
BspOI GCTAGC 2 cut(s) 797, 2118
BspPI GGATC 6 cut(s) 942, 955, 1300, 2191, 2288, 3773
BspT104I TTCGAA 1 cut(s) 3044
BspTI CTTAAG 1 cut(s) 3617
BspTNI GGTCTC 2 cut(s) 2750, 2960
BsrBI CCGCTC 1 cut(s) 2181
BsrDI GCAATG 4 cut(s) 198, 1009, 1441, 1883
BsrFI RCCGGY 1 cut(s) 3441
BsrI ACTGG 7 cut(s) 1368, 1935, 2360, 2504, 2592, 2877, 4052
BssAI RCCGGY 1 cut(s) 3441
BssECI CCNNGG 3 cut(s) 2062, 2412, 2701
BssNI GRCGYC 4 cut(s) 88, 277, 437, 2864
BssSI CACGAG 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 2701
Bst2BI CACGAG 1 cut(s) 63
Bst2UI CCWGG 4 cut(s) 594, 1405, 2125, 4032
Bst4CI ACNGT 9 cut(s) 955, 1248, 1779, 1931, 2370, 3104, 3173, 3426, 3559
Bst6I CTCTTC 1 cut(s) 3915
BstACI GRCGYC 4 cut(s) 88, 277, 437, 2864
BstAFI CTTAAG 1 cut(s) 3617
BstAPI GCANNNNNTGC 2 cut(s) 932, 4071
BstBAI YACGTR 1 cut(s) 920
BstBI TTCGAA 1 cut(s) 3044
BstC8I GCNNGC 9 cut(s) 795, 1426, 1890, 2116, 2453, 2492, 3137, 3374, 3945
BstDEI CTNAG 7 cut(s) 74, 215, 361, 447, 1740, 2106, 3365
BstDSI CCRYGG 2 cut(s) 2412, 2701
BstENI CCTNNNNNAGG 1 cut(s) 4035
BstF5I GGATG 8 cut(s) 214, 688, 1323, 1534, 2116, 2648, 2697, 3947
BstFNI CGCG 4 cut(s) 641, 1083, 1477, 2684
BstH2I RGCGCY 2 cut(s) 2717, 3389
BstHHI GCGC 4 cut(s) 1477, 2716, 2732, 3388
BstMAI GTCTC 8 cut(s) 62, 213, 2750, 2960, 3183, 3618, 3753, 4085
BstMCI CGRYCG 2 cut(s) 3768, 3996
BstNI CCWGG 4 cut(s) 594, 1405, 2125, 4032
BstNSI RCATGY 5 cut(s) 461, 626, 843, 2218, 2494
BstPAI GACNNNNGTC 1 cut(s) 3963
BstSCI CCNGG 5 cut(s) 592, 1200, 1403, 2123, 4030
BstSFI CTRYAG 4 cut(s) 1485, 2981, 3422, 3568
BstSLI GKGCMC 1 cut(s) 2582
BstUI CGCG 4 cut(s) 641, 1083, 1477, 2684
BstV2I GAAGAC 3 cut(s) 1109, 2859, 3600
BstX2I RGATCY 3 cut(s) 947, 2293, 3778
BstXI CCANNNNNNTGG 1 cut(s) 1942
BstYI RGATCY 3 cut(s) 947, 2293, 3778
BsuI GTATCC 2 cut(s) 499, 682
BsuRI GGCC 7 cut(s) 1036, 1598, 2123, 3135, 3372, 3525, 4051
BtgI CCRYGG 2 cut(s) 2412, 2701
BtrI CACGTC 2 cut(s) 3612, 3747
BtsCI GGATG 8 cut(s) 214, 688, 1323, 1534, 2116, 2648, 2697, 3947
BtsI GCAGTG 2 cut(s) 1384, 3840
BtsIMutI CAGTG 8 cut(s) 951, 1384, 1942, 2497, 2870, 3169, 3362, 3840
BveI ACCTGC 1 cut(s) 2974
Cac8I GCNNGC 9 cut(s) 795, 1426, 1890, 2116, 2453, 2492, 3137, 3374, 3945
CaiI CAGNNNCTG 1 cut(s) 3919
CfoI GCGC 4 cut(s) 1477, 2716, 2732, 3388
Cfr10I RCCGGY 1 cut(s) 3441
Cfr13I GGNCC 9 cut(s) 488, 737, 1786, 2046, 2121, 2314, 2426, 3523, 4050
CseI GACGC 6 cut(s) 79, 96, 170, 285, 445, 647
Csp6I GTAC 1 cut(s) 152
CspAI ACCGGT 1 cut(s) 3441
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 7 cut(s) 74, 215, 361, 447, 1740, 2106, 3365
DraI TTTAAA 1 cut(s) 426
DraIII CACNNNGTG 1 cut(s) 554
Eam1104I CTCTTC 1 cut(s) 3915
EarI CTCTTC 1 cut(s) 3915
Ecl136II GAGCTC 3 cut(s) 360, 2245, 3280
Eco130I CCWWGG 1 cut(s) 2701
Eco24I GRGCYC 3 cut(s) 362, 2247, 3282
Eco31I GGTCTC 2 cut(s) 2750, 2960
Eco47I GGWCC 6 cut(s) 488, 737, 1786, 2046, 2314, 2426
Eco47III AGCGCT 1 cut(s) 2715
Eco53kI GAGCTC 3 cut(s) 360, 2245, 3280
Eco57I CTGAAG 3 cut(s) 2462, 3893, 3896
Eco72I CACGTG 1 cut(s) 920
EcoICRI GAGCTC 3 cut(s) 360, 2245, 3280
EcoNI CCTNNNNNAGG 1 cut(s) 4035
EcoO109I RGGNCCY 3 cut(s) 488, 2046, 3523
EcoRI GAATTC 4 cut(s) 124, 749, 758, 2432
EcoRII CCWGG 4 cut(s) 592, 1403, 2123, 4030
EcoT14I CCWWGG 1 cut(s) 2701
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 3 cut(s) 362, 2247, 3282
ErhI CCWWGG 1 cut(s) 2701
Esp3I CGTCTC 4 cut(s) 62, 3618, 3753, 4085
FalI AAGNNNNNCTT 4 cut(s) 30, 62, 3698, 3730
FaqI GGGAC 5 cut(s) 448, 856, 2032, 2080, 2817
FauI CCCGC 3 cut(s) 1897, 2111, 4070
FauNDI CATATG 1 cut(s) 1551
FbaI TGATCA 2 cut(s) 982, 2020
FokI GGATG 8 cut(s) 221, 695, 1330, 1521, 2123, 2655, 2684, 3934
FriOI GRGCYC 3 cut(s) 362, 2247, 3282
FspBI CTAG 8 cut(s) 8, 113, 794, 1964, 2115, 2550, 2727, 3113
GlaI GCGC 4 cut(s) 1476, 2715, 2731, 3387
GsuI CTGGAG 2 cut(s) 615, 2609
HaeII RGCGCY 2 cut(s) 2717, 3389
HaeIII GGCC 7 cut(s) 1036, 1598, 2123, 3135, 3372, 3525, 4051
HapII CCGG 5 cut(s) 746, 1202, 3442, 3518, 3590
HgaI GACGC 6 cut(s) 79, 96, 170, 285, 445, 647
HhaI GCGC 4 cut(s) 1477, 2716, 2732, 3388
Hin1I GRCGYC 4 cut(s) 88, 277, 437, 2864
Hin6I GCGC 4 cut(s) 1475, 2714, 2730, 3386
HinP1I GCGC 4 cut(s) 1475, 2714, 2730, 3386
HincII GTYRAC 5 cut(s) 57, 180, 562, 2740, 4028
HindII GTYRAC 5 cut(s) 57, 180, 562, 2740, 4028
HindIII AAGCTT 4 cut(s) 2323, 2627, 3005, 3549
HpaII CCGG 5 cut(s) 746, 1202, 3442, 3518, 3590
HphI GGTGA 6 cut(s) 456, 1478, 2239, 3529, 3579, 3895
Hpy166II GTNNAC 7 cut(s) 57, 180, 562, 1130, 2740, 3035, 4028
Hpy8I GTNNAC 7 cut(s) 57, 180, 562, 1130, 2740, 3035, 4028
Hpy99I CGWCG 1 cut(s) 279
HpyCH4III ACNGT 9 cut(s) 955, 1248, 1779, 1931, 2370, 3104, 3173, 3426, 3559
HpyCH4IV ACGT 7 cut(s) 919, 1123, 2864, 3037, 3185, 3611, 3746
HpyF3I CTNAG 7 cut(s) 74, 215, 361, 447, 1740, 2106, 3365
HpySE526I ACGT 7 cut(s) 919, 1123, 2864, 3037, 3185, 3611, 3746
Hsp92I GRCGYC 4 cut(s) 88, 277, 437, 2864
HspAI GCGC 4 cut(s) 1475, 2714, 2730, 3386
KflI GGGWCCC 1 cut(s) 2046
Ksp22I TGATCA 2 cut(s) 982, 2020
LmnI GCTCC 7 cut(s) 790, 887, 2782, 2795, 2857, 3277, 3831
MaeI CTAG 8 cut(s) 8, 113, 794, 1964, 2115, 2550, 2727, 3113
MaeII ACGT 7 cut(s) 919, 1123, 2864, 3037, 3185, 3611, 3746
MaeIII GTNAC 9 cut(s) 3, 42, 387, 462, 703, 910, 1379, 1672, 3585
MbiI CCGCTC 1 cut(s) 2181
MfeI CAATTG 2 cut(s) 962, 2332
MflI RGATCY 3 cut(s) 947, 2293, 3778
MhlI GDGCHC 5 cut(s) 362, 2247, 2582, 3282, 3324
MlyI GAGTC 2 cut(s) 1099, 3910
MmeI TCCRAC 5 cut(s) 660, 865, 2344, 2620, 3702
Mph1103I ATGCAT 1 cut(s) 1455
MroXI GAANNNNTTC 1 cut(s) 3597
MslI CAYNNNNRTG 3 cut(s) 627, 2685, 3096
MspA1I CMGCKG 1 cut(s) 1490
MspCI CTTAAG 1 cut(s) 3617
MspI CCGG 5 cut(s) 746, 1202, 3442, 3518, 3590
MspR9I CCNGG 5 cut(s) 594, 1202, 1405, 2125, 4032
MunI CAATTG 2 cut(s) 962, 2332
Mva1269I GAATGC 4 cut(s) 1194, 1956, 3891, 4065
MvaI CCWGG 4 cut(s) 594, 1405, 2125, 4032
MvnI CGCG 4 cut(s) 641, 1083, 1477, 2684
NciI CCSGG 1 cut(s) 1202
NcoI CCATGG 1 cut(s) 2701
NdeI CATATG 1 cut(s) 1551
NheI GCTAGC 2 cut(s) 793, 2114
NlaIV GGNNCC 7 cut(s) 490, 949, 2047, 2048, 2122, 2784, 2988
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 6 cut(s) 3, 462, 703, 910, 1379, 3585
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 5 cut(s) 461, 626, 843, 2218, 2494
NspV TTCGAA 1 cut(s) 3044
OliI CACNNNNGTG 1 cut(s) 2685
PaeI GCATGC 1 cut(s) 2494
PciI ACATGT 2 cut(s) 839, 2214
PcsI WCGNNNNNNNCGW 1 cut(s) 3050
PctI GAATGC 4 cut(s) 1194, 1956, 3891, 4065
PdmI GAANNNNTTC 1 cut(s) 3597
PfeI GAWTC 8 cut(s) 722, 1333, 1701, 1771, 1978, 2150, 2473, 3205
PflMI CCANNNNNTGG 3 cut(s) 2363, 3131, 3806
PinAI ACCGGT 1 cut(s) 3441
Ple19I CGATCG 2 cut(s) 3768, 3996
PleI GAGTC 2 cut(s) 1099, 3910
PmaCI CACGTG 1 cut(s) 920
PmlI CACGTG 1 cut(s) 920
PpsI GAGTC 2 cut(s) 1099, 3910
Ppu21I YACGTR 1 cut(s) 920
PpuMI RGGWCCY 2 cut(s) 488, 2046
PscI ACATGT 2 cut(s) 839, 2214
PshAI GACNNNNGTC 1 cut(s) 3963
PshBI ATTAAT 1 cut(s) 1911
Psp124BI GAGCTC 3 cut(s) 362, 2247, 3282
Psp5II RGGWCCY 2 cut(s) 488, 2046
Psp6I CCWGG 4 cut(s) 592, 1403, 2123, 4030
PspCI CACGTG 1 cut(s) 920
PspGI CCWGG 4 cut(s) 592, 1403, 2123, 4030
PspN4I GGNNCC 7 cut(s) 490, 949, 2047, 2048, 2122, 2784, 2988
PspPI GGNCC 9 cut(s) 488, 737, 1786, 2046, 2121, 2314, 2426, 3523, 4050
PspPPI RGGWCCY 2 cut(s) 488, 2046
PstI CTGCAG 3 cut(s) 1489, 2985, 3572
PstNI CAGNNNCTG 1 cut(s) 3919
PsuI RGATCY 3 cut(s) 947, 2293, 3778
PvuI CGATCG 2 cut(s) 3768, 3996
PvuII CAGCTG 1 cut(s) 1490
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
RseI CAYNNNNRTG 3 cut(s) 627, 2685, 3096
SacI GAGCTC 3 cut(s) 362, 2247, 3282
Sau96I GGNCC 9 cut(s) 488, 737, 1786, 2046, 2121, 2314, 2426, 3523, 4050
SchI GAGTC 2 cut(s) 1099, 3910
ScrFI CCNGG 5 cut(s) 594, 1202, 1405, 2125, 4032
SduI GDGCHC 5 cut(s) 362, 2247, 2582, 3282, 3324
SfcI CTRYAG 4 cut(s) 1485, 2981, 3422, 3568
SfuI TTCGAA 1 cut(s) 3044
SinI GGWCC 6 cut(s) 488, 737, 1786, 2046, 2314, 2426
SmiMI CAYNNNNRTG 3 cut(s) 627, 2685, 3096
SmlI CTYRAG 5 cut(s) 355, 2080, 2630, 3317, 3617
SmoI CTYRAG 5 cut(s) 355, 2080, 2630, 3317, 3617
SphI GCATGC 1 cut(s) 2494
SspI AATATT 1 cut(s) 2776
SspMI CTAG 8 cut(s) 8, 113, 794, 1964, 2115, 2550, 2727, 3113
SstI GAGCTC 3 cut(s) 362, 2247, 3282
StyD4I CCNGG 5 cut(s) 592, 1200, 1403, 2123, 4030
StyI CCWWGG 1 cut(s) 2701
TaaI ACNGT 9 cut(s) 955, 1248, 1779, 1931, 2370, 3104, 3173, 3426, 3559
TaiI ACGT 7 cut(s) 922, 1126, 2867, 3040, 3188, 3614, 3749
TauI GCSGC 6 cut(s) 1039, 1636, 1921, 2767, 2788, 3712
TfiI GAWTC 8 cut(s) 722, 1333, 1701, 1771, 1978, 2150, 2473, 3205
TscAI CASTG 8 cut(s) 958, 1384, 1942, 2504, 2877, 3176, 3369, 3847
TseFI GTSAC 6 cut(s) 3, 462, 703, 910, 1379, 3585
Tsp45I GTSAC 6 cut(s) 3, 462, 703, 910, 1379, 3585
TspDTI ATGAA 8 cut(s) 209, 557, 1494, 1746, 2268, 2691, 3285, 3705
TspGWI ACGGA 4 cut(s) 421, 664, 2306, 2454
TspRI CASTG 8 cut(s) 958, 1384, 1942, 2504, 2877, 3176, 3369, 3847
Van91I CCANNNNNTGG 3 cut(s) 2363, 3131, 3806
Vha464I CTTAAG 1 cut(s) 3617
VpaK11BI GGWCC 6 cut(s) 488, 737, 1786, 2046, 2314, 2426
VspI ATTAAT 1 cut(s) 1911
XagI CCTNNNNNAGG 1 cut(s) 4035
XapI RAATTY 9 cut(s) 124, 341, 749, 758, 1042, 1589, 2273, 2432, 3684
XceI RCATGY 5 cut(s) 461, 626, 843, 2218, 2494
XmnI GAANNNNTTC 1 cut(s) 3597
XspI CTAG 8 cut(s) 8, 113, 794, 1964, 2115, 2550, 2727, 3113
ZraI GACGTC 1 cut(s) 2865
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.