RchiOBHm_Chr2g0131121

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000070
Physical Location & Seq
Forward (+)
47190375 .. 47195851
5477 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50248

Sequence Viewer

Length: 3273 bp
ATGACACTACAGTCCCCTTTTCATGCTGGTGCACCAGGAAGTAAAATAATTGTGACAACACGTGATGCAGGAGTTGCAAAAATAGTGAGAGCCATTGATGTTCACAATTTGGACTATATATCAAATGATGATTGTTGGAAAGTATTCAAGCAACATGCACTCTTGGATGTTAGTAATTGCGGACCACAGAACTTTGAGGTATTTCGGGGGCAAATTGTTGCCAAATGCGGTGGATTACCTTTGGCTGCAAGAACTCTTGGTGGTCTTTTAGGTTGTAAAGAAATACATGAATGGGGAGAAATACTTGAAAACAAGCTGTGGAGTCTATCTGATAATAGTAACATTCTCCCTGTGTTAAATTTGAGCTATCATTATCTCCCTTCAGATTTGAAAAGGTGTTTTGCTTATTGCTCGATACTTCCACAAGATTATGAATTTGAGGAAATGCAGTTGATCCTCCTATGGATGGCAGAGGATTTAATTCCACAACCGAAAGACAACAAACAATTGGAAGATTTAGGTGCTGATTATTTTCGGGAGTTGTTATCTAGATCATTCTTTCAAAATTCAAGCAAAAGCAATGCACGATATGTAATGCATGATCTCATTAGTGATTTGGCAAGAAAGATCACAGGAGCGATATGTTTTAGATTGGAGGACGCACAGAATAGTTACAGGCAAATTAGATCTTCTCCGAAGGCTCGTCAATTGTCTTACATTTCTGGCATATCTGATGGTGTTCAAAAATTCGAAGCCTTTGATCAAGTCAAACATCTGCGGACTTTTCTACCATTTACTGTTTCAACACTGCCAGGTTTTCGACATTGTTTGACTCGTAAGATCACTCATGATCTAGTGCCAAAACTGCCATACCTAAGGGTGTTATCTTTTAATGGCTATGGAATCACAGCTCTGCCAAAATCGATAGGGGAGTTGAAGTCTTTACGGTATCTTGATGTTTCTCATGCAGTAATAAAGGATTTTCCTGAATCTGCCAGTACTCTTTACAACTTACAAACATTGATAGTAAATGATTGTAATTTTTTGAGAACACTACCTACAAACATGAGGAATCTAATCAGTTTGCGTCATCTTCACAATTCGAAACTATATTTGTTGGAAAGAATGCCTGGCCAACTTGGTAGTCTGACAAATCTGCAAACATTGAATAATTTTCTGGTGGCCGAAAGTGGAGATTCAGGGATAAGTCAGATCGGTCCCCTATTGCATCTCCGGGGGACATTGCATCTTTCAAGATTGGAGAATGTGAATGATGCGGAGGATGCAAAGATGGCCAATTGTATTAAGAAGGACGAGCTTGATGAGTTGGTACTCGAATGGAGTGAAAACAGAGAAATGAGACTAGATGTGCTTGACATGTTGCAACCTCACAAAAACCTCAAAATGCTCACAGTAAGGGGTTTCAGGGGATTGCAATTTTCAGCATGGATTGGAGATTCTTTATTCTCTAAAATGGTGGTGGTGAGGTTAGAAAATTGTAGAAACGGCCAGCTCTTGCCACCACTTGGGCAATTGTGTTCTCTGAAAGAGCTATATATAAGAAAAATGCCTGGAGTGGTAAATGTAGGGCAGGAGTTTTATGGGGAGGGTAGCTTGCCTTTCCCTCGATTAGAAATTCTGGAATTTGAGCGGATGGACCTTTGGAAGGAGTGGTTTTCCTATCAACAAGATCAGGGACTTGAAACTTTCCCTTGCTTGAAAATGCTTTCCATCAAAAGCTGCTGGAAACTGCAGGGTCGGTTACCTGAGAACCTTCATTTACTGTCCACGCTTGTGATTCATCAATGTGAGCAGCTAGTGGTTTCAATCACAAATTATACAAGCCTTTGTGAATTGAAAATAAATAATTGCAGACAGGTGATCCATAGAAGTGCTGCTCAGTTTGAGTCGCTAGAGGCCCTAGAGCTTTCAAGTGTTTCAGAGTTCGTACTCCAAGCAGAGGAATTTGTGAAAGGGTTGACAAAAACTAAAGATCTGAAGATTACAGTTTCTAAGAAGCCGACATTTTTAAGGGAGAATGAGGATAGATTACAGGAAAACCTGATTTCTGTTCCTCATTTCTTCATTGGAAGCAACTCCCACCTTTTTTTGTTTCATTACTTATCATCACTTCAGAAACTTCACATATACGACTGCCCTAGTATAATTTCTTTTGAAGAGGTTTGTTTTCCACCTTTTCTTGCAGAAATAGAGATTATGCGTTGCCATTCTCTTGTGTATTTCGCAAGATACCAGATTTCCCCACAACTGAGAAGATTAAGTATCTGGGTTTGCAAAAATTTTAAATCAGTAGTAGAGATTGAAGTAGGAAGTACTCAGACTTCATCTTGTTGGTCTTCCTCATCTTCTGCTTCTACTCCTTGTATGATGCAAAAGGAGACAACTAGTCTCGAGGACTTGGAAATAATTAGTTGTCCCTCTATGACGTCCTTAGGCTACCTACTAATCAGGACACTTAAACATCTTCGGATATCGGCCTGTGAAAATATGGAGAAAATAGTCGAAAGGTTCCATGATAACATTCTTCTTGAAGAACTTCAATTATGGACATGTCCAAATCTTAAATCCTTCCCGGAGGGTCTATGCCACCTTACCAATCTTCTATCACTATCGATCATAAACTGTGGAAGTTTTGTTTCCTTTCCAACAGAAGTGTTTCCAAGAAGCGCCTTCAACTTGAGGAAGATAGAGATTTCCAACTGCCCTAATTTGGAGGGCATCCCCAAAGGCTTGCAGAACCTGAACTCTCTTCAGGACTTTCATGGTTTGGCGTCATTTTTAGATGAGGGTTTTCCCACCAATCTAACAAGACTAGTAGTTTGGAAAGTCAAGAATTGCAAGGCGCTATTGGAGTTGGGATTGCACAGACTCACCTCTCTTAGAGAGTTGTATATCAGTGGTGAAGATCCAGATTTGGTGTCCTTTCCACCCGAGGACAAGATGACACCACCCCTGCCCAAATCTCTCATTCAAATCGCAATTGCCGACTTCCCAAATCTTACTAAACTCAGCGAAGGCTTTCAATTCCTCACCTCTCTTGAATATCTTGAAGTATCTTCTTGTCCTAAACTAGCATCAATTCCAGAAGGGGGTTTGCCTCTTTCACTTAATCGACTTTATGTCATTCAATGTCCTCTGCTACAAGAGAGGTGCAAACCAATGAAAGGTAGATACTGGCCCTCTATAGCCCACATCCCTTGCATAACTAATGGAGGCATGGACATCCGGTTCTACTCCCTCCAAGAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1090

Amino Acids

124.01

Weight (kDa)

6.58

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 139 - 207 3.7e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 288 - 559 2.4e-19 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 402 - 523 8.5e-31 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AatII GACGTC 1 cut(s) 2450
AccB7I CCANNNNNTGG 1 cut(s) 1526
AccBSI CCGCTC 1 cut(s) 1651
AciI CCGC 5 cut(s) 180, 228, 778, 1277, 1651
AclWI GGATC 3 cut(s) 448, 1876, 2921
AcoI YGGCCR 4 cut(s) 1132, 1182, 1293, 1507
AcsI RAATTY 8 cut(s) 358, 434, 565, 746, 1635, 1643, 1964, 2299
AcuI CTGAAG 4 cut(s) 366, 2018, 2117, 2756
AcvI CACGTG 1 cut(s) 62
AcyI GRCGYC 2 cut(s) 2447, 2792
AfaI GTAC 4 cut(s) 1000, 1332, 1950, 2335
AfiI CCNNNNNNNGG 7 cut(s) 466, 1526, 1666, 1960, 2731, 3110, 3185
AflIII ACRYGT 3 cut(s) 59, 1377, 2570
AhdI GACNNNNNGTC 2 cut(s) 2406, 3140
AhlI ACTAGT 2 cut(s) 2405, 2833
AjnI CCWGG 4 cut(s) 34, 811, 1129, 1570
AleI CACNNNNGTG 1 cut(s) 1793
AloI GAACNNNNNNTCC 2 cut(s) 3233, 3265
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 3 cut(s) 1354, 2393, 2414
Alw44I GTGCAC 1 cut(s) 30
AlwI GGATC 3 cut(s) 448, 1876, 2921
AlwNI CAGNNNCTG 1 cut(s) 2761
Ama87I CYCGRG 2 cut(s) 2411, 2951
AoxI GGCC 7 cut(s) 1132, 1182, 1293, 1507, 1917, 2496, 3197
ApaLI GTGCAC 1 cut(s) 30
ApeKI GCWGC 4 cut(s) 245, 1740, 1813, 1895
ApoI RAATTY 8 cut(s) 358, 434, 565, 746, 1635, 1643, 1964, 2299
ArsI GACNNNNNNTTYG 2 cut(s) 2823, 2855
Asp700I GAANNNNTTC 4 cut(s) 143, 2556, 2676, 3039
AspLEI GCGC 2 cut(s) 2690, 2866
AspS9I GGNCC 5 cut(s) 182, 1217, 1657, 1918, 3198
AsuC2I CCSGG 2 cut(s) 1235, 2594
AsuHPI GGTGA 5 cut(s) 1495, 1891, 2884, 2933, 3043
AsuII TTCGAA 2 cut(s) 750, 1103
AvaI CYCGRG 2 cut(s) 2411, 2951
AvaII GGWCC 3 cut(s) 182, 1217, 1657
AxyI CCTNAGG 2 cut(s) 875, 2452
BaeGI GKGCMC 1 cut(s) 34
BalI TGGCCA 2 cut(s) 1134, 1295
BarI GAAGNNNNNNTAC 2 cut(s) 2326, 2358
BbrPI CACGTG 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 2349
Bbv12I GWGCWC 1 cut(s) 34
BbvI GCAGC 4 cut(s) 232, 1727, 1825, 1882
BccI CCATC 5 cut(s) 460, 728, 1285, 1648, 1739
BceAI ACGGC 1 cut(s) 1522
BciT130I CCWGG 4 cut(s) 36, 813, 1131, 1572
BclI TGATCA 1 cut(s) 760
BcnI CCSGG 2 cut(s) 1235, 2594
BcoDI GTCTC 3 cut(s) 1354, 2393, 2414
BcuI ACTAGT 2 cut(s) 2405, 2833
BfmI CTRYAG 3 cut(s) 8, 1751, 3204
BfoI RGCGCY 2 cut(s) 2691, 2867
BglII AGATCT 2 cut(s) 686, 1993
BisI GCNGC 4 cut(s) 246, 1741, 1814, 1896
BlsI GCNGC 4 cut(s) 247, 1742, 1815, 1897
BmcAI AGTACT 2 cut(s) 1000, 2335
Bme1390I CCNGG 6 cut(s) 36, 813, 1131, 1235, 1572, 2594
Bme18I GGWCC 3 cut(s) 182, 1217, 1657
BmeRI GACNNNNNGTC 2 cut(s) 2406, 3140
BmeT110I CYCGRG 2 cut(s) 2411, 2951
BmgT120I GGNCC 5 cut(s) 182, 1217, 1657, 1918, 3198
BmiI GGNNCC 2 cut(s) 1219, 2531
BmrFI CCNGG 6 cut(s) 36, 813, 1131, 1235, 1572, 2594
BmsI GCATC 8 cut(s) 55, 1237, 1255, 1264, 1273, 2379, 2748, 3104
BpiI GAAGAC 1 cut(s) 2349
BpmI CTGGAG 1 cut(s) 1593
Bpu14I TTCGAA 2 cut(s) 750, 1103
BpuEI CTTGAG 1 cut(s) 2719
BpuMI CCSGG 2 cut(s) 1235, 2594
Bsa29I ATCGAT 2 cut(s) 923, 2633
BsaAI YACGTR 1 cut(s) 62
BsaHI GRCGYC 2 cut(s) 2447, 2792
BsaJI CCNNGG 2 cut(s) 1234, 2952
BsaWI WCCGGW 1 cut(s) 3246
BsaXI ACNNNNNCTCC 2 cut(s) 923, 953
Bsc4I CCNNNNNNNGG 7 cut(s) 466, 1526, 1666, 1960, 2731, 3110, 3185
Bse1I ACTGG 2 cut(s) 996, 3200
Bse21I CCTNAGG 2 cut(s) 875, 2452
Bse3DI GCAATG 2 cut(s) 586, 1241
BseBI CCWGG 4 cut(s) 36, 813, 1131, 1572
BseCI ATCGAT 2 cut(s) 923, 2633
BseDI CCNNGG 2 cut(s) 1234, 2952
BseGI GGATG 7 cut(s) 172, 471, 1288, 1659, 2739, 3213, 3243
BseLI CCNNNNNNNGG 7 cut(s) 466, 1526, 1666, 1960, 2731, 3110, 3185
BseMI GCAATG 2 cut(s) 586, 1241
BseMII CTCAG 5 cut(s) 1758, 1913, 2261, 2351, 3043
BseNI ACTGG 2 cut(s) 996, 3200
BseSI GKGCMC 1 cut(s) 34
BseXI GCAGC 4 cut(s) 232, 1727, 1825, 1882
BshFI GGCC 7 cut(s) 1134, 1184, 1295, 1509, 1919, 2498, 3199
BshVI ATCGAT 2 cut(s) 923, 2633
BsiHKAI GWGCWC 1 cut(s) 34
BsiHKCI CYCGRG 2 cut(s) 2411, 2951
BsiSI CCGG 3 cut(s) 1234, 2594, 3247
BslFI GGGAC 4 cut(s) 1203, 1252, 1710, 2421
BslI CCNNNNNNNGG 7 cut(s) 466, 1526, 1666, 1960, 2731, 3110, 3185
BsmAI GTCTC 3 cut(s) 1354, 2393, 2414
BsmFI GGGAC 4 cut(s) 1203, 1252, 1710, 2421
BsmI GAATGC 1 cut(s) 1131
BsnI GGCC 7 cut(s) 1134, 1184, 1295, 1509, 1919, 2498, 3199
BsoBI CYCGRG 2 cut(s) 2411, 2951
Bsp119I TTCGAA 2 cut(s) 750, 1103
Bsp1286I GDGCHC 1 cut(s) 34
BspACI CCGC 5 cut(s) 180, 228, 778, 1277, 1651
BspANI GGCC 7 cut(s) 1134, 1184, 1295, 1509, 1919, 2498, 3199
BspCNI CTCAG 5 cut(s) 1759, 1912, 2262, 2350, 3042
BspDI ATCGAT 2 cut(s) 923, 2633
BspHI TCATGA 1 cut(s) 847
BspLI GGNNCC 2 cut(s) 1219, 2531
BspMAI CTGCAG 1 cut(s) 1755
BspPI GGATC 3 cut(s) 448, 1876, 2921
BspT104I TTCGAA 2 cut(s) 750, 1103
BsrBI CCGCTC 1 cut(s) 1651
BsrDI GCAATG 2 cut(s) 586, 1241
BsrI ACTGG 2 cut(s) 996, 3200
BssECI CCNNGG 2 cut(s) 1234, 2952
BssNI GRCGYC 2 cut(s) 2447, 2792
Bst2UI CCWGG 4 cut(s) 36, 813, 1131, 1572
Bst4CI ACNGT 7 cut(s) 12, 799, 948, 1414, 1785, 2008, 2645
Bst6I CTCTTC 2 cut(s) 2172, 2775
BstACI GRCGYC 2 cut(s) 2447, 2792
BstAPI GCANNNNNTGC 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 62
BstBI TTCGAA 2 cut(s) 750, 1103
BstC8I GCNNGC 3 cut(s) 1511, 1616, 2753
BstDEI CTNAG 9 cut(s) 875, 1767, 1899, 2013, 2270, 2337, 2452, 2900, 3029
BstEII GGTNACC 1 cut(s) 1761
BstENI CCTNNNNNAGG 1 cut(s) 1664
BstF5I GGATG 7 cut(s) 172, 471, 1288, 1659, 2739, 3213, 3243
BstH2I RGCGCY 2 cut(s) 2691, 2867
BstHHI GCGC 2 cut(s) 2690, 2866
BstMAI GTCTC 3 cut(s) 1354, 2393, 2414
BstMWI GCNNNNNNNGC 4 cut(s) 74, 865, 1283, 1292
BstNI CCWGG 4 cut(s) 36, 813, 1131, 1572
BstNSI RCATGY 3 cut(s) 158, 1381, 2574
BstPI GGTNACC 1 cut(s) 1761
BstSCI CCNGG 6 cut(s) 34, 811, 1129, 1233, 1570, 2592
BstSFI CTRYAG 3 cut(s) 8, 1751, 3204
BstSLI GKGCMC 1 cut(s) 34
BstV1I GCAGC 4 cut(s) 232, 1727, 1825, 1882
BstV2I GAAGAC 1 cut(s) 2349
BstX2I RGATCY 3 cut(s) 686, 1993, 2926
BstYI RGATCY 3 cut(s) 686, 1993, 2926
Bsu15I ATCGAT 2 cut(s) 923, 2633
Bsu36I CCTNAGG 2 cut(s) 875, 2452
BsuRI GGCC 7 cut(s) 1134, 1184, 1295, 1509, 1919, 2498, 3199
BsuTUI ATCGAT 2 cut(s) 923, 2633
BtsCI GGATG 7 cut(s) 172, 471, 1288, 1659, 2739, 3213, 3243
BtsI GCAGTG 1 cut(s) 806
BtsIMutI CAGTG 2 cut(s) 806, 2923
Cac8I GCNNGC 3 cut(s) 1511, 1616, 2753
CaiI CAGNNNCTG 1 cut(s) 2761
CciI TCATGA 1 cut(s) 847
CfoI GCGC 2 cut(s) 2690, 2866
Cfr13I GGNCC 5 cut(s) 182, 1217, 1657, 1918, 3198
ClaI ATCGAT 2 cut(s) 923, 2633
CseI GACGC 3 cut(s) 668, 1076, 2781
Csp6I GTAC 4 cut(s) 999, 1331, 1949, 2334
CspCI CAANNNNNGTGG 4 cut(s) 211, 246, 3200, 3235
CviQI GTAC 4 cut(s) 999, 1331, 1949, 2334
DdeI CTNAG 9 cut(s) 875, 1767, 1899, 2013, 2270, 2337, 2452, 2900, 3029
DraI TTTAAA 1 cut(s) 2305
DrdI GACNNNNNNGTC 1 cut(s) 10
DriI GACNNNNNGTC 2 cut(s) 2406, 3140
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 4 cut(s) 1132, 1182, 1293, 1507
Eam1104I CTCTTC 2 cut(s) 2172, 2775
Eam1105I GACNNNNNGTC 2 cut(s) 2406, 3140
EarI CTCTTC 2 cut(s) 2172, 2775
Eco32I GATATC 1 cut(s) 2493
Eco47I GGWCC 3 cut(s) 182, 1217, 1657
Eco57I CTGAAG 4 cut(s) 366, 2018, 2117, 2756
Eco72I CACGTG 1 cut(s) 62
Eco81I CCTNAGG 2 cut(s) 875, 2452
Eco88I CYCGRG 2 cut(s) 2411, 2951
Eco91I GGTNACC 1 cut(s) 1761
EcoNI CCTNNNNNAGG 1 cut(s) 1664
EcoO109I RGGNCCY 1 cut(s) 1918
EcoO65I GGTNACC 1 cut(s) 1761
EcoRII CCWGG 4 cut(s) 34, 811, 1129, 1570
EcoRV GATATC 1 cut(s) 2493
EcoT22I ATGCAT 1 cut(s) 600
FaqI GGGAC 4 cut(s) 1203, 1252, 1710, 2421
FbaI TGATCA 1 cut(s) 760
Fnu4HI GCNGC 4 cut(s) 246, 1741, 1814, 1896
FokI GGATG 7 cut(s) 179, 478, 1295, 1666, 2726, 3200, 3230
Fsp4HI GCNGC 4 cut(s) 246, 1741, 1814, 1896
GlaI GCGC 2 cut(s) 2689, 2865
GluI GCNGC 4 cut(s) 246, 1741, 1814, 1896
GsuI CTGGAG 1 cut(s) 1593
HaeII RGCGCY 2 cut(s) 2691, 2867
HaeIII GGCC 7 cut(s) 1134, 1184, 1295, 1509, 1919, 2498, 3199
HapII CCGG 3 cut(s) 1234, 2594, 3247
HgaI GACGC 3 cut(s) 668, 1076, 2781
HhaI GCGC 2 cut(s) 2690, 2866
Hin1I GRCGYC 2 cut(s) 2447, 2792
Hin6I GCGC 2 cut(s) 2688, 2864
HinP1I GCGC 2 cut(s) 2688, 2864
HincII GTYRAC 1 cut(s) 1980
HindII GTYRAC 1 cut(s) 1980
HpaII CCGG 3 cut(s) 1234, 2594, 3247
HphI GGTGA 5 cut(s) 1495, 1891, 2884, 2933, 3043
Hpy166II GTNNAC 4 cut(s) 32, 103, 1788, 1980
Hpy8I GTNNAC 4 cut(s) 32, 103, 1788, 1980
HpyAV CCTTC 9 cut(s) 390, 691, 1303, 1660, 1784, 2599, 2701, 3029, 3101
HpyCH4III ACNGT 7 cut(s) 12, 799, 948, 1414, 1785, 2008, 2645
HpyCH4IV ACGT 2 cut(s) 61, 2447
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 865, 1283, 1292
HpyF3I CTNAG 9 cut(s) 875, 1767, 1899, 2013, 2270, 2337, 2452, 2900, 3029
HpySE526I ACGT 2 cut(s) 61, 2447
Hsp92I GRCGYC 2 cut(s) 2447, 2792
HspAI GCGC 2 cut(s) 2688, 2864
Ksp22I TGATCA 1 cut(s) 760
LmnI GCTCC 1 cut(s) 635
Lsp1109I GCAGC 4 cut(s) 232, 1727, 1825, 1882
LweI GCATC 8 cut(s) 55, 1237, 1255, 1264, 1273, 2379, 2748, 3104
MaeII ACGT 2 cut(s) 61, 2447
MaeIII GTNAC 4 cut(s) 52, 338, 671, 1761
MbiI CCGCTC 1 cut(s) 1651
MfeI CAATTG 5 cut(s) 506, 707, 1297, 1532, 3000
MflI RGATCY 3 cut(s) 686, 1993, 2926
MhlI GDGCHC 1 cut(s) 34
MlsI TGGCCA 2 cut(s) 1134, 1295
MluNI TGGCCA 2 cut(s) 1134, 1295
MlyI GAGTC 4 cut(s) 331, 826, 1916, 2883
MmeI TCCRAC 4 cut(s) 116, 1098, 2690, 2742
Mox20I TGGCCA 2 cut(s) 1134, 1295
Mph1103I ATGCAT 1 cut(s) 600
MroXI GAANNNNTTC 4 cut(s) 143, 2556, 2676, 3039
MscI TGGCCA 2 cut(s) 1134, 1295
MslI CAYNNNNRTG 4 cut(s) 27, 1793, 1806, 1890
Msp20I TGGCCA 2 cut(s) 1134, 1295
MspI CCGG 3 cut(s) 1234, 2594, 3247
MspR9I CCNGG 6 cut(s) 36, 813, 1131, 1235, 1572, 2594
MunI CAATTG 5 cut(s) 506, 707, 1297, 1532, 3000
Mva1269I GAATGC 1 cut(s) 1131
MvaI CCWGG 4 cut(s) 36, 813, 1131, 1572
MwoI GCNNNNNNNGC 4 cut(s) 74, 865, 1283, 1292
NciI CCSGG 2 cut(s) 1235, 2594
NlaIV GGNNCC 2 cut(s) 1219, 2531
NmuCI GTSAC 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 600
NspI RCATGY 3 cut(s) 158, 1381, 2574
NspV TTCGAA 2 cut(s) 750, 1103
OliI CACNNNNGTG 1 cut(s) 1793
PaeR7I CTCGAG 1 cut(s) 2411
PagI TCATGA 1 cut(s) 847
PciI ACATGT 2 cut(s) 1377, 2570
PcsI WCGNNNNNNNCGW 1 cut(s) 3003
PctI GAATGC 1 cut(s) 1131
PdmI GAANNNNTTC 4 cut(s) 143, 2556, 2676, 3039
PfeI GAWTC 6 cut(s) 903, 989, 1072, 1196, 1457, 1798
PflMI CCANNNNNTGG 1 cut(s) 1526
PfoI TCCNGGA 1 cut(s) 2592
PkrI GCNGC 4 cut(s) 247, 1742, 1815, 1897
PleI GAGTC 4 cut(s) 330, 826, 1915, 2883
PmaCI CACGTG 1 cut(s) 62
PmlI CACGTG 1 cut(s) 62
PpsI GAGTC 4 cut(s) 330, 826, 1915, 2883
Ppu21I YACGTR 1 cut(s) 62
PscI ACATGT 2 cut(s) 1377, 2570
Psp6I CCWGG 4 cut(s) 34, 811, 1129, 1570
PspCI CACGTG 1 cut(s) 62
PspEI GGTNACC 1 cut(s) 1761
PspGI CCWGG 4 cut(s) 34, 811, 1129, 1570
PspN4I GGNNCC 2 cut(s) 1219, 2531
PspPI GGNCC 5 cut(s) 182, 1217, 1657, 1918, 3198
PsrI GAACNNNNNNTAC 2 cut(s) 1042, 1074
PstI CTGCAG 1 cut(s) 1755
PstNI CAGNNNCTG 1 cut(s) 2761
PsuI RGATCY 3 cut(s) 686, 1993, 2926
RsaI GTAC 4 cut(s) 1000, 1332, 1950, 2335
RsaNI GTAC 4 cut(s) 999, 1331, 1949, 2334
RseI CAYNNNNRTG 4 cut(s) 27, 1793, 1806, 1890
SatI GCNGC 4 cut(s) 246, 1741, 1814, 1896
Sau96I GGNCC 5 cut(s) 182, 1217, 1657, 1918, 3198
ScaI AGTACT 2 cut(s) 1000, 2335
SchI GAGTC 4 cut(s) 331, 826, 1916, 2883
ScrFI CCNGG 6 cut(s) 36, 813, 1131, 1235, 1572, 2594
SduI GDGCHC 1 cut(s) 34
SfaNI GCATC 8 cut(s) 55, 1237, 1255, 1264, 1273, 2379, 2748, 3104
SfcI CTRYAG 3 cut(s) 8, 1751, 3204
Sfr274I CTCGAG 1 cut(s) 2411
SfuI TTCGAA 2 cut(s) 750, 1103
SinI GGWCC 3 cut(s) 182, 1217, 1657
SlaI CTCGAG 1 cut(s) 2411
SmiMI CAYNNNNRTG 4 cut(s) 27, 1793, 1806, 1890
SmlI CTYRAG 2 cut(s) 2411, 2698
SmoI CTYRAG 2 cut(s) 2411, 2698
SpeI ACTAGT 2 cut(s) 2405, 2833
SsiI CCGC 5 cut(s) 180, 228, 778, 1277, 1651
StyD4I CCNGG 6 cut(s) 34, 811, 1129, 1233, 1570, 2592
TaaI ACNGT 7 cut(s) 12, 799, 948, 1414, 1785, 2008, 2645
TaiI ACGT 2 cut(s) 64, 2450
TaqII GACCGA 1 cut(s) 1205
TatI WGTACW 2 cut(s) 998, 2333
TfiI GAWTC 6 cut(s) 903, 989, 1072, 1196, 1457, 1798
TscAI CASTG 2 cut(s) 813, 2923
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 4 cut(s) 245, 1740, 1813, 1895
Tsp45I GTSAC 1 cut(s) 52
TspRI CASTG 2 cut(s) 813, 2923
Van91I CCANNNNNTGG 1 cut(s) 1526
VneI GTGCAC 1 cut(s) 30
VpaK11BI GGWCC 3 cut(s) 182, 1217, 1657
XagI CCTNNNNNAGG 1 cut(s) 1664
XapI RAATTY 8 cut(s) 358, 434, 565, 746, 1635, 1643, 1964, 2299
XbaI TCTAGA 1 cut(s) 548
XceI RCATGY 3 cut(s) 158, 1381, 2574
XhoI CTCGAG 1 cut(s) 2411
XmnI GAANNNNTTC 4 cut(s) 143, 2556, 2676, 3039
ZraI GACGTC 1 cut(s) 2448
ZrmI AGTACT 2 cut(s) 1000, 2335
Zsp2I ATGCAT 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.