MD11G1043500.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
3724133 .. 3734235
10103 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1043500.v1.1.491

Sequence Viewer

Length: 4098 bp
ATGTCACTGGGAGAGGTCTTTATCTCTGGTTTACTTCAAGTGTTACTTGAAAGGTTAGCATCTCGTGAGACACTAAGCTACTTTAGACGCCTACTTGGCGTTGGCAAAGAGCTGGAGAAATGGAATTCAATGTTGTCTACTATTCGAGCGGTGCTGAATGACGCGGAGGAAAAGCAATTGACGAGCCAAGCAGTAAAATTGTGGTTGGATGATCTCAGAAACTTGGCTTATAATGTGGAAGACATATTGGACAGGTTTTCAACCGAGATGTTGCAGCGCCAAATAAAAGAGAAACATGGTGCTACCACAAGCAAGGTATGGGGATTGTTTTCTGATGTTAAATTTAACCGTAACTTGATCTCAGAAATAAAGAAGATTACTGATCGGTTACATGAGATATTGGAACAAAAAAACCAACTTGGTTTACATGAGGGGACACCATCTGTTAAGGCATGGCACATGCCACCAAGTTCCCATTTACCAGAAGGACCTATGATTGGAAGGGATACAGACAAGACGAACATTATTGACTTCTTGTTGAAAGAAGCACTCCGTGCTGTTAACTTTCATGTAGTTGCCATTGTCGGTATGCCTGGAGTGGGAAAGACAACACTTGCAAGACATATTTATGATGATGACGAGGTGAAAGAGTTTAACCAAAAAGTATGGGTATCTGTGTCGGATGACTTCAATCTTGAAAGAGTGACAAAGGCAATTCTTGAGGCTGCCACATCTGGTCTTGCAAAGGATTTCAAGGAATTCAGCCAGGTTCAAGAAAGTTTGAGGAATGAGCTAGCAGGAAAAAAGTTTCTAATTGTTCTAGACGATGTTTGGGACACATGTGTCTATGATTTGTGGATAAAATTGCAGTCTCCTTTTCGTGTTGGAGCACCAGGAAGTAAGATAGTTGTGACAACACGTGATACAAATGTTGCAAACATGATGGGGTCCACTATTTATCGATTGGGTGGCATATCAGATGATCAATGTTGGGAAGTCTTTGAGCAGCATTCCCTCTTGGACATTACCAAAAGGCCGCAAAATTTTAAGTTGATAAAGGAGAAAGTTGTTGCAAAATGTCGCGGATTGCCATTGGTTGCAAGGACTCTTGGAGGTCTTCTACGTTGTAAACAAGTAGAAGAATGGGAGGCAATCTTAAACAACAAGATGTGGAGTCTATCAGACAATAGTGGCATTCTCCCAGTATTAAAATTGAGCTATCACCATCTCCCTTCGAGTTTGAAACGATGCTTTGCCTATTGTTCTCTACTTCCAAATGACTATGAATTTAGGGAGAAGCAGTTGATCCTTCTGTGGATGGCAGAAGGTTTGATTCAACAAAGAGTAGAGGACAAGAAACAGCTGGAAGAAGTAGGCAGTGACTATTTTCAAGAGCTATTGTCCAGGTCATTATTTCAAAAGGCAAGCAAAAGCAATGATAAGTATGTAATGCATGACCTTGTTGGTGAGTTGGCACGGTGGGCTGCAGGTGAAATATGCTTTTCATTGGAGGACAAGGGAAATGTTGACTTGCATCCAATGGCTCGTCATATGTCTTATGTCAGTGGTTACTTGGATGGGGTTAAAAAATTTGAGGCCATATCCAGAGTTAAACGTTTGAGGACATTCCTGTCATTTCGGCCTTCAGAATTTGATAAGAATTATCTAACTCGTTGGGTTACATTTGACCTATTGCCAGAAATGCGAATCTTACGAGTGCTTTCTTTGAATGGTTATGGAATAACTGAGTTGCCCAATTCAATTGGTAAATTGTGGAATCTACGGTATCTGGACCTTTCTTACACAGACATAACGAGTTTGCCTCCATCAACAACCACTCTTTGCAATCTGCAAACATTGTTATTAGAAGGTTGTTTTCAATTGAAGGCTTTGCCCGCAAACATGAGTAATCTAATTAGTTTGCGGCATCTCAACAGTTTTCGTGGATTGGAAGGAATGCCTCCGCATCTAGGTCGATTGACGAATCTGCAATCCTTGTCATATTTTGTGGTAGGCAAAGGTAGTGATCAGTCTGGGATAAGAGAGATAGGGTCCCTATCACATCTTCAAGGGACATTGCACCTCTCAAGATTGGAGAATGCGGTTGATGCTAAGGATGCTAGTGGGGCCAAGTTAATAGACAAGGAGGGGCTTGATTCATTGAAGCTGGAATGGTCTAATTTGAGCAGATCGAGGGAAATGGAGTTGGGTGTGCTTGACATGTTACAACCTCCTAAAAATCTCAAAGAGCTCACTATTATGGGTTATGCTGGATTGAAATTTTCATCGTGGATTGGAGATCCTCTGTTCTCTAAAATGGTGTGTGTAAGCTTATACAATTGTAGAAATTGTCATCTCTTGCCACCAGTTGGACAGTTTCCTTGCCTTAAAGAACTTCATATCACGAGAATGACTTCTGTGGAAATCGTTGGTCCCGAATTCTACGGAGAGGGTAGCTTGCCTTTTCGAGTATTAGAGATTCTTGAGTTTTTGGAGATGGGGCATTGGGAGAAATGGCTGCCTTTCGATCACGATAAGGGAAGTGGTGTTTTCCCTAGCTTAAAAGTGCTTTCAATCAAAGAGTGCCCTAAACTGGAGGGTGAGTTGCCGAAGAAACTTAATTCGTTAGCAAAGCTTGAGATTGCTAGATGCAAGGAATTGGTGGTTGAGATTGACAATTATGAACACACGAGTGAACTAAGCATCGATGGTGGCAAAGCGCTAGTGCATACTACCGCTGAGGTTGACTTTAAGTTTTTAGACACCTTGTGTCTTTCAAACATTTTGGAGCCGCGATTGCAAGCAAGGGGACTTACGAAAGCATTAGGCAAGATTAAGAAGTTGAACATTAGTGGTTGTGAGGAGCTGACGTCTTCATTGAAAAATGAGGACGGATGTTTCCCACAACTGATTTCTCTTGTTCGTTTGGTTATTGAACGCAACTCTGCCCTTGTTGAAGATCTCGGAAAAGAATTGGAGGGGTTGCTGCAGGTTCCAATATTAGCTTGTAAGCTTGAATATCTGGAAATAAACTATTGTGGAAGTCTTTCGAAGCTGCCGAAGGGGTTGCGTCAGTTGTCGTCTCTTCAAGAGCTTCACATACGGCAATGTTCAAGTCTAGTTTCTTTTCCAGATGTTGGCCTGCCACCTTCTCTTAAAGTCTTGGAGATTAGGAATTGTGACTCTCTGATGTATATTGCAAAATATCAGATTCCCCAAAATCTGAAAAGAATAGAGATATGGAGGTGTGGAAGTTTGAAATCATTAGTGGAGGAGGAGAATGAGCTCGTTGTTTCTTGTTCATCTTCCTTTTCTGTTTCTCTTGAGCACTTGCGGATATCTCTTTGTCCATCTCTGACATCGTTATCACCGAGAGGCCAGCTTTACAGGGCGCTTAAACACCTTGAGATAGAGGAATGTGAAGGGCTAGAGTTAATAACATCAGAGCGGTTCTTCCCCGACAATACTAATAATTGTCTTGAATGTATTAACATTGCCAGGTGTCAAAATCTGAAATCCTTACCGGAGGGCCTATGCCACCTCACCAATCTTCAAGCTTTTATTGTTAGGGAATGCAGAAAATTGGAGGTGTCACCGGAAGACATTCGCAATCTCACGTCTCTTAAAGTATTGGAGATTGGCTACATGGAAGGTTTGCCTTCCTTTCCTCCCAACCTCACATCACTTGAAATTTATGGAGCCAAGAGTTGTAAGCAGCTCTGGGAATCGGAGTGGGGGTTGGACAAACTCACGTCTCTCAAATATTTGTCGATCAAAAGTGAAGATCCAGATGTGTTGTCATTTCCACCTGATGGGAAGGAGGAGATGCTGCTCCCCAATTCTCTCACTGAACTCATAATTGATGGCTTCCCATATCTGAAGAAACTCGGCAATGGCATTCAATTCCTCACCTCTCTTCAGACGCTGCAACTATTTGATTGTCCAAAGCTCGCATCCATTCCAGAGGAAGGCTTGCCTCTTTCACTTGGGAAACTTCGGATCGACCGGTGTCCTCTACTACAAGAGAGATTTCAACCTGGAAAAGGACGATACTGGCCCAAAATATCCCACATTCCTTTCCTAAAAATAGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1366

Amino Acids

154.62

Weight (kDa)

6.39

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 99 2.2e-23 Rx N-terminal domain
NB-ARC PF00931 171 - 337 2.1e-33 NB-ARC domain
WHD_DRP PF23559 424 - 492 4e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 565 - 860 1.1e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 680 - 804 1.5e-34 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 996 - 1141 8.3e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 1128 - 1261 2.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
AarI CACCTGC 1 cut(s) 1478
AasI GACNNNNNNGTC 2 cut(s) 842, 1630
AatII GACGTC 1 cut(s) 2873
Acc36I ACCTGC 2 cut(s) 1478, 2980
AccB7I CCANNNNNTGG 3 cut(s) 2369, 3137, 3812
AccBSI CCGCTC 2 cut(s) 149, 3448
AccI GTMKAC 1 cut(s) 137
AccII CGCG 3 cut(s) 164, 1083, 2794
AclI AACGTT 1 cut(s) 1615
AclWI GGATC 4 cut(s) 1300, 2294, 3779, 4007
AcuI CTGAAG 3 cut(s) 1629, 3899, 3902
AcvI CACGTG 1 cut(s) 920
AcyI GRCGYC 2 cut(s) 88, 2870
AdeI CACNNNGTG 2 cut(s) 554, 2769
AfeI AGCGCT 1 cut(s) 2721
AfiI CCNNNNNNNGG 9 cut(s) 497, 599, 1970, 2369, 2593, 3137, 3812, 3968, 4043
AflIII ACRYGT 3 cut(s) 839, 917, 2220
AgeI ACCGGT 1 cut(s) 4005
AjiI CACGTC 2 cut(s) 3618, 3753
AjnI CCWGG 6 cut(s) 592, 765, 892, 1403, 3497, 4036
AleI CACNNNNGTG 1 cut(s) 2691
Alw21I GWGCWC 4 cut(s) 892, 2253, 3288, 3330
Alw26I GTCTC 5 cut(s) 62, 876, 3087, 3624, 3759
AlwI GGATC 4 cut(s) 1300, 2294, 3779, 4007
AlwNI CAGNNNCTG 1 cut(s) 3925
Aor51HI AGCGCT 1 cut(s) 2721
AoxI GGCC 8 cut(s) 1034, 1596, 1640, 2127, 3139, 3376, 3529, 4055
AsiGI ACCGGT 1 cut(s) 4005
Asp700I GAANNNNTTC 3 cut(s) 2413, 3046, 3603
AspLEI GCGC 3 cut(s) 279, 2722, 3394
AspS9I GGNCC 8 cut(s) 488, 948, 1792, 2052, 2127, 2432, 3529, 4056
AsuHPI GGTGA 9 cut(s) 655, 1214, 1478, 1502, 2612, 3360, 3535, 3585, 3901
AsuII TTCGAA 1 cut(s) 3050
AsuNHI GCTAGC 1 cut(s) 793
AvaII GGWCC 5 cut(s) 488, 948, 1792, 2052, 2432
BaeGI GKGCMC 1 cut(s) 2588
BaeI ACNNNNGTAYC 2 cut(s) 915, 948
BanII GRGCYC 2 cut(s) 2253, 3288
BauI CACGAG 3 cut(s) 63, 2404, 2689
BbrPI CACGTG 1 cut(s) 920
BbsI GAAGAC 4 cut(s) 246, 1109, 2865, 3606
Bbv12I GWGCWC 4 cut(s) 892, 2253, 3288, 3330
BbvCI CCTCAGC 1 cut(s) 2739
BceAI ACGGC 1 cut(s) 3119
BcgI CGANNNNNNTGC 4 cut(s) 1955, 1989, 3049, 3083
BciT130I CCWGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 2 cut(s) 982, 2026
BcoDI GTCTC 5 cut(s) 62, 876, 3087, 3624, 3759
BfaI CTAG 9 cut(s) 794, 821, 1970, 2121, 2556, 2646, 2723, 3119, 3428
BfmI CTRYAG 2 cut(s) 1485, 2987
BfoI RGCGCY 3 cut(s) 280, 2723, 3395
BfuAI ACCTGC 2 cut(s) 1478, 2980
BfuI GTATCC 1 cut(s) 499
BglI GCCNNNNNGGC 1 cut(s) 96
BglII AGATCT 1 cut(s) 2959
Bme1390I CCNGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
Bme18I GGWCC 5 cut(s) 488, 948, 1792, 2052, 2432
BmgBI CACGTC 2 cut(s) 3618, 3753
BmgT120I GGNCC 8 cut(s) 488, 948, 1792, 2052, 2127, 2432, 3529, 4056
BmiI GGNNCC 8 cut(s) 949, 2053, 2054, 2128, 2434, 2790, 2994, 3700
BmrFI CCNGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
BmrI ACTGGG 2 cut(s) 17, 1196
BmtI GCTAGC 1 cut(s) 797
BmuI ACTGGG 2 cut(s) 17, 1196
BoxI GACNNNNGTC 1 cut(s) 4008
BpiI GAAGAC 4 cut(s) 246, 1109, 2865, 3606
BplI GAGNNNNNCTC 2 cut(s) 1807, 1839
BpmI CTGGAG 3 cut(s) 134, 615, 2615
Bpu10I CCTNAGC 2 cut(s) 2112, 2739
Bpu14I TTCGAA 1 cut(s) 3050
BpuEI CTTGAG 6 cut(s) 740, 2071, 2504, 2657, 3344, 3425
Bsa29I ATCGAT 2 cut(s) 961, 2706
BsaAI YACGTR 1 cut(s) 920
BsaHI GRCGYC 2 cut(s) 88, 2870
BsaWI WCCGGW 3 cut(s) 3523, 3595, 4005
BsaXI ACNNNNNCTCC 8 cut(s) 1286, 1316, 2588, 2618, 2855, 2885, 3722, 3752
Bsc4I CCNNNNNNNGG 9 cut(s) 497, 599, 1970, 2369, 2593, 3137, 3812, 3968, 4043
Bse118I RCCGGY 1 cut(s) 4005
Bse1I ACTGG 5 cut(s) 12, 1202, 2366, 2598, 4058
Bse3DI GCAATG 5 cut(s) 1441, 2075, 3113, 3492, 3898
BseBI CCWGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
BseCI ATCGAT 2 cut(s) 961, 2706
BseGI GGATG 8 cut(s) 214, 688, 1323, 1534, 1582, 2122, 2900, 3953
BseLI CCNNNNNNNGG 9 cut(s) 497, 599, 1970, 2369, 2593, 3137, 3812, 3968, 4043
BseMI GCAATG 5 cut(s) 1441, 2075, 3113, 3492, 3898
BseMII CTCAG 4 cut(s) 229, 375, 1737, 2730
BseNI ACTGG 5 cut(s) 12, 1202, 2366, 2598, 4058
BseRI GAGGAG 4 cut(s) 2876, 3287, 3290, 3836
BseSI GKGCMC 1 cut(s) 2588
Bsh1236I CGCG 3 cut(s) 164, 1083, 2794
Bsh1285I CGRYCG 1 cut(s) 4006
BshFI GGCC 8 cut(s) 1036, 1598, 1642, 2129, 3141, 3378, 3531, 4057
BshTI ACCGGT 1 cut(s) 4005
BshVI ATCGAT 2 cut(s) 961, 2706
BsiEI CGRYCG 1 cut(s) 4006
BsiHKAI GWGCWC 4 cut(s) 892, 2253, 3288, 3330
BsiSI CCGG 3 cut(s) 3524, 3596, 4006
BslFI GGGAC 6 cut(s) 448, 848, 2038, 2086, 2418, 2823
BslI CCNNNNNNNGG 9 cut(s) 497, 599, 1970, 2369, 2593, 3137, 3812, 3968, 4043
BsmAI GTCTC 5 cut(s) 62, 876, 3087, 3624, 3759
BsmBI CGTCTC 3 cut(s) 3087, 3624, 3759
BsmFI GGGAC 6 cut(s) 448, 848, 2038, 2086, 2418, 2823
BsmI GAATGC 6 cut(s) 1009, 1194, 1962, 2104, 3578, 3897
BsnI GGCC 8 cut(s) 1036, 1598, 1642, 2129, 3141, 3378, 3531, 4057
Bsp119I TTCGAA 1 cut(s) 3050
Bsp1286I GDGCHC 5 cut(s) 892, 2253, 2588, 3288, 3330
BspANI GGCC 8 cut(s) 1036, 1598, 1642, 2129, 3141, 3378, 3531, 4057
BspCNI CTCAG 4 cut(s) 228, 374, 1738, 2731
BspDI ATCGAT 2 cut(s) 961, 2706
BspFNI CGCG 3 cut(s) 164, 1083, 2794
BspLI GGNNCC 8 cut(s) 949, 2053, 2054, 2128, 2434, 2790, 2994, 3700
BspMAI CTGCAG 2 cut(s) 1489, 2991
BspMI ACCTGC 2 cut(s) 1478, 2980
BspOI GCTAGC 1 cut(s) 797
BspPI GGATC 4 cut(s) 1300, 2294, 3779, 4007
BspT104I TTCGAA 1 cut(s) 3050
BsrBI CCGCTC 2 cut(s) 149, 3448
BsrDI GCAATG 5 cut(s) 1441, 2075, 3113, 3492, 3898
BsrFI RCCGGY 1 cut(s) 4005
BsrI ACTGG 5 cut(s) 12, 1202, 2366, 2598, 4058
BssAI RCCGGY 1 cut(s) 4005
BssNI GRCGYC 2 cut(s) 88, 2870
BssSI CACGAG 3 cut(s) 63, 2404, 2689
Bst2BI CACGAG 3 cut(s) 63, 2404, 2689
Bst2UI CCWGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
Bst4CI ACNGT 5 cut(s) 350, 1479, 1785, 1937, 2376
Bst6I CTCTTC 2 cut(s) 3090, 3921
BstACI GRCGYC 2 cut(s) 88, 2870
BstAPI GCANNNNNTGC 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 920
BstBI TTCGAA 1 cut(s) 3050
BstC8I GCNNGC 9 cut(s) 795, 1426, 1896, 2459, 2802, 3143, 3380, 3951, 3974
BstDEI CTNAG 7 cut(s) 74, 215, 361, 1746, 2112, 2699, 2739
BstENI CCTNNNNNAGG 1 cut(s) 4041
BstF5I GGATG 8 cut(s) 214, 688, 1323, 1534, 1582, 2122, 2900, 3953
BstFNI CGCG 3 cut(s) 164, 1083, 2794
BstH2I RGCGCY 3 cut(s) 280, 2723, 3395
BstHHI GCGC 3 cut(s) 279, 2722, 3394
BstMAI GTCTC 5 cut(s) 62, 876, 3087, 3624, 3759
BstMCI CGRYCG 1 cut(s) 4006
BstMWI GCNNNNNNNGC 9 cut(s) 96, 554, 1481, 1702, 1895, 2108, 2117, 2126, 2797
BstNI CCWGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
BstNSI RCATGY 3 cut(s) 463, 843, 2224
BstPAI GACNNNNGTC 1 cut(s) 4008
BstSCI CCNGG 6 cut(s) 592, 765, 892, 1403, 3497, 4036
BstSFI CTRYAG 2 cut(s) 1485, 2987
BstSLI GKGCMC 1 cut(s) 2588
BstUI CGCG 3 cut(s) 164, 1083, 2794
BstV2I GAAGAC 4 cut(s) 246, 1109, 2865, 3606
BstX2I RGATCY 3 cut(s) 2299, 2959, 3784
BstYI RGATCY 3 cut(s) 2299, 2959, 3784
Bsu15I ATCGAT 2 cut(s) 961, 2706
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 8 cut(s) 1036, 1598, 1642, 2129, 3141, 3378, 3531, 4057
BsuTUI ATCGAT 2 cut(s) 961, 2706
BtrI CACGTC 2 cut(s) 3618, 3753
BtsCI GGATG 8 cut(s) 214, 688, 1323, 1534, 1582, 2122, 2900, 3953
BtsI GCAGTG 1 cut(s) 1384
BtsIMutI CAGTG 4 cut(s) 5, 1384, 1570, 3846
BveI ACCTGC 2 cut(s) 1478, 2980
Cac8I GCNNGC 9 cut(s) 795, 1426, 1896, 2459, 2802, 3143, 3380, 3951, 3974
CaiI CAGNNNCTG 1 cut(s) 3925
CfoI GCGC 3 cut(s) 279, 2722, 3394
Cfr10I RCCGGY 1 cut(s) 4005
Cfr13I GGNCC 8 cut(s) 488, 948, 1792, 2052, 2127, 2432, 3529, 4056
ClaI ATCGAT 2 cut(s) 961, 2706
CseI GACGC 4 cut(s) 96, 170, 3059, 3931
CspAI ACCGGT 1 cut(s) 4005
DdeI CTNAG 7 cut(s) 74, 215, 361, 1746, 2112, 2699, 2739
DraIII CACNNNGTG 2 cut(s) 554, 2769
DrdI GACNNNNNNGTC 2 cut(s) 842, 1630
DseDI GACNNNNNNGTC 2 cut(s) 842, 1630
Eam1104I CTCTTC 2 cut(s) 3090, 3921
EarI CTCTTC 2 cut(s) 3090, 3921
Ecl136II GAGCTC 2 cut(s) 2251, 3286
Eco24I GRGCYC 2 cut(s) 2253, 3288
Eco32I GATATC 1 cut(s) 3339
Eco47I GGWCC 5 cut(s) 488, 948, 1792, 2052, 2432
Eco47III AGCGCT 1 cut(s) 2721
Eco53kI GAGCTC 2 cut(s) 2251, 3286
Eco57I CTGAAG 3 cut(s) 1629, 3899, 3902
Eco72I CACGTG 1 cut(s) 920
EcoICRI GAGCTC 2 cut(s) 2251, 3286
EcoNI CCTNNNNNAGG 1 cut(s) 4041
EcoO109I RGGNCCY 3 cut(s) 488, 2052, 3529
EcoRI GAATTC 3 cut(s) 124, 758, 2438
EcoRII CCWGG 6 cut(s) 592, 765, 892, 1403, 3497, 4036
EcoRV GATATC 1 cut(s) 3339
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 2 cut(s) 2253, 3288
Esp3I CGTCTC 3 cut(s) 3087, 3624, 3759
FalI AAGNNNNNCTT 2 cut(s) 30, 62
FaqI GGGAC 6 cut(s) 448, 848, 2038, 2086, 2418, 2823
FauI CCCGC 1 cut(s) 1903
FauNDI CATATG 1 cut(s) 1551
FbaI TGATCA 2 cut(s) 982, 2026
FblI GTMKAC 1 cut(s) 137
FokI GGATG 8 cut(s) 221, 695, 1330, 1521, 1589, 2129, 2907, 3940
FriOI GRGCYC 2 cut(s) 2253, 3288
FspBI CTAG 9 cut(s) 794, 821, 1970, 2121, 2556, 2646, 2723, 3119, 3428
GlaI GCGC 3 cut(s) 278, 2721, 3393
GsuI CTGGAG 3 cut(s) 134, 615, 2615
HaeII RGCGCY 3 cut(s) 280, 2723, 3395
HaeIII GGCC 8 cut(s) 1036, 1598, 1642, 2129, 3141, 3378, 3531, 4057
HapII CCGG 3 cut(s) 3524, 3596, 4006
HgaI GACGC 4 cut(s) 96, 170, 3059, 3931
HhaI GCGC 3 cut(s) 279, 2722, 3394
Hin1I GRCGYC 2 cut(s) 88, 2870
Hin6I GCGC 3 cut(s) 277, 2720, 3392
HinP1I GCGC 3 cut(s) 277, 2720, 3392
HincII GTYRAC 3 cut(s) 562, 1528, 2746
HindII GTYRAC 3 cut(s) 562, 1528, 2746
HindIII AAGCTT 4 cut(s) 2329, 2633, 3011, 3555
HpaI GTTAAC 1 cut(s) 562
HpaII CCGG 3 cut(s) 3524, 3596, 4006
HphI GGTGA 9 cut(s) 655, 1214, 1478, 1502, 2612, 3360, 3535, 3585, 3901
Hpy166II GTNNAC 9 cut(s) 32, 138, 425, 562, 951, 1130, 1528, 2696, 2746
Hpy8I GTNNAC 9 cut(s) 32, 138, 425, 562, 951, 1130, 1528, 2696, 2746
HpyCH4III ACNGT 5 cut(s) 350, 1479, 1785, 1937, 2376
HpyCH4IV ACGT 6 cut(s) 919, 1123, 1615, 2870, 3617, 3752
HpyF10VI GCNNNNNNNGC 9 cut(s) 96, 554, 1481, 1702, 1895, 2108, 2117, 2126, 2797
HpyF3I CTNAG 7 cut(s) 74, 215, 361, 1746, 2112, 2699, 2739
HpySE526I ACGT 6 cut(s) 919, 1123, 1615, 2870, 3617, 3752
Hsp92I GRCGYC 2 cut(s) 88, 2870
HspAI GCGC 3 cut(s) 277, 2720, 3392
KflI GGGWCCC 1 cut(s) 2052
Ksp22I TGATCA 2 cut(s) 982, 2026
KspAI GTTAAC 1 cut(s) 562
LmnI GCTCC 5 cut(s) 887, 2788, 2863, 3698, 3837
MaeI CTAG 9 cut(s) 794, 821, 1970, 2121, 2556, 2646, 2723, 3119, 3428
MaeII ACGT 6 cut(s) 919, 1123, 1615, 2870, 3617, 3752
MbiI CCGCTC 2 cut(s) 149, 3448
MfeI CAATTG 4 cut(s) 176, 1761, 1880, 2338
MflI RGATCY 3 cut(s) 2299, 2959, 3784
MhlI GDGCHC 5 cut(s) 892, 2253, 2588, 3288, 3330
MlyI GAGTC 3 cut(s) 1099, 1183, 3176
MmeI TCCRAC 5 cut(s) 186, 660, 865, 2350, 3720
Mph1103I ATGCAT 1 cut(s) 1455
MroXI GAANNNNTTC 3 cut(s) 2413, 3046, 3603
MslI CAYNNNNRTG 4 cut(s) 627, 2258, 2408, 2691
MspA1I CMGCKG 2 cut(s) 1363, 2738
MspI CCGG 3 cut(s) 3524, 3596, 4006
MspR9I CCNGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
MunI CAATTG 4 cut(s) 176, 1761, 1880, 2338
Mva1269I GAATGC 6 cut(s) 1009, 1194, 1962, 2104, 3578, 3897
MvaI CCWGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
MvnI CGCG 3 cut(s) 164, 1083, 2794
MwoI GCNNNNNNNGC 9 cut(s) 96, 554, 1481, 1702, 1895, 2108, 2117, 2126, 2797
NdeI CATATG 1 cut(s) 1551
NheI GCTAGC 1 cut(s) 793
NlaIV GGNNCC 8 cut(s) 949, 2053, 2054, 2128, 2434, 2790, 2994, 3700
NmeAIII GCCGAG 1 cut(s) 3867
NmuCI GTSAC 6 cut(s) 3, 703, 910, 1379, 3179, 3591
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 3 cut(s) 463, 843, 2224
NspV TTCGAA 1 cut(s) 3050
OliI CACNNNNGTG 1 cut(s) 2691
PaqCI CACCTGC 1 cut(s) 1478
PciI ACATGT 2 cut(s) 839, 2220
PcsI WCGNNNNNNNCGW 3 cut(s) 2433, 3056, 3368
PctI GAATGC 6 cut(s) 1009, 1194, 1962, 2104, 3578, 3897
PdmI GAANNNNTTC 3 cut(s) 2413, 3046, 3603
PfeI GAWTC 8 cut(s) 1333, 1707, 1777, 1984, 2156, 2479, 3211, 3725
PflMI CCANNNNNTGG 3 cut(s) 2369, 3137, 3812
PinAI ACCGGT 1 cut(s) 4005
PleI GAGTC 3 cut(s) 1099, 1182, 3176
PmaCI CACGTG 1 cut(s) 920
PmlI CACGTG 1 cut(s) 920
PpsI GAGTC 3 cut(s) 1099, 1182, 3176
Ppu21I YACGTR 1 cut(s) 920
PpuMI RGGWCCY 2 cut(s) 488, 2052
PscI ACATGT 2 cut(s) 839, 2220
PshAI GACNNNNGTC 1 cut(s) 4008
PsiI TTATAA 1 cut(s) 231
Psp124BI GAGCTC 2 cut(s) 2253, 3288
Psp1406I AACGTT 1 cut(s) 1615
Psp5II RGGWCCY 2 cut(s) 488, 2052
Psp6I CCWGG 6 cut(s) 592, 765, 892, 1403, 3497, 4036
PspCI CACGTG 1 cut(s) 920
PspGI CCWGG 6 cut(s) 592, 765, 892, 1403, 3497, 4036
PspN4I GGNNCC 8 cut(s) 949, 2053, 2054, 2128, 2434, 2790, 2994, 3700
PspPI GGNCC 8 cut(s) 488, 948, 1792, 2052, 2127, 2432, 3529, 4056
PspPPI RGGWCCY 2 cut(s) 488, 2052
PstI CTGCAG 2 cut(s) 1489, 2991
PstNI CAGNNNCTG 1 cut(s) 3925
PsuI RGATCY 3 cut(s) 2299, 2959, 3784
PvuII CAGCTG 1 cut(s) 1363
RseI CAYNNNNRTG 4 cut(s) 627, 2258, 2408, 2691
SacI GAGCTC 2 cut(s) 2253, 3288
Sau96I GGNCC 8 cut(s) 488, 948, 1792, 2052, 2127, 2432, 3529, 4056
SchI GAGTC 3 cut(s) 1099, 1183, 3176
ScrFI CCNGG 6 cut(s) 594, 767, 894, 1405, 3499, 4038
SduI GDGCHC 5 cut(s) 892, 2253, 2588, 3288, 3330
SfcI CTRYAG 2 cut(s) 1485, 2987
SfuI TTCGAA 1 cut(s) 3050
SinI GGWCC 5 cut(s) 488, 948, 1792, 2052, 2432
SmiMI CAYNNNNRTG 4 cut(s) 627, 2258, 2408, 2691
SmlI CTYRAG 6 cut(s) 719, 2086, 2483, 2636, 3323, 3404
SmoI CTYRAG 6 cut(s) 719, 2086, 2483, 2636, 3323, 3404
SspI AATATT 2 cut(s) 3000, 3764
SspMI CTAG 9 cut(s) 794, 821, 1970, 2121, 2556, 2646, 2723, 3119, 3428
SstI GAGCTC 2 cut(s) 2253, 3288
StyD4I CCNGG 6 cut(s) 592, 765, 892, 1403, 3497, 4036
TaaI ACNGT 5 cut(s) 350, 1479, 1785, 1937, 2376
TaiI ACGT 6 cut(s) 922, 1126, 1618, 2873, 3620, 3755
TauI GCSGC 3 cut(s) 1039, 1927, 2794
TfiI GAWTC 8 cut(s) 1333, 1707, 1777, 1984, 2156, 2479, 3211, 3725
TscAI CASTG 4 cut(s) 12, 1384, 1570, 3853
TseFI GTSAC 6 cut(s) 3, 703, 910, 1379, 3179, 3591
Tsp45I GTSAC 6 cut(s) 3, 703, 910, 1379, 3179, 3591
TspDTI ATGAA 9 cut(s) 557, 1299, 1494, 2148, 2274, 2387, 2697, 2865, 3291
TspGWI ACGGA 3 cut(s) 542, 2460, 2907
TspRI CASTG 4 cut(s) 12, 1384, 1570, 3853
Van91I CCANNNNNTGG 3 cut(s) 2369, 3137, 3812
VpaK11BI GGWCC 5 cut(s) 488, 948, 1792, 2052, 2432
XagI CCTNNNNNAGG 1 cut(s) 4041
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 3 cut(s) 463, 843, 2224
XmiI GTMKAC 1 cut(s) 137
XmnI GAANNNNTTC 3 cut(s) 2413, 3046, 3603
XspI CTAG 9 cut(s) 794, 821, 1970, 2121, 2556, 2646, 2723, 3119, 3428
ZraI GACGTC 1 cut(s) 2871
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.