Rw5G026370

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000070
Physical Location & Seq
Reverse (-)
38143756 .. 38147830
4075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026370.1

Sequence Viewer

Length: 4014 bp
ATGGCAGTGGGAGAGGTCTTTCTTGCTGCGTTCCTTCAGGTGCTATTCGACAGGTTAGCCTCTCGTGAGTTCCTCAAATTTGCACGGCAGAAGGGCATTGGCGGGAAGCTTGAAAAATGGAGTGAAACACTGTCTGCAATTCGAGCAGTGTTGTATGACGCCGAGGAGAAGCAACTCATAAGCATGTCAGTGAAAGAGTGGCTGGATGATCTCAAAGACTTGGCTTATGATGTGGAGGACATCTTGGATAGGTTTTCCACTGACATGTTACGATTCAAGGCCAAGGAGCAAAATCTGGCTGGCACAAGCAAGGTACGAGGCCTCTTTCTTAAAGTTAAATGCAACTTCAATATGAACTCTGAAATGGAAAAGATTTCTGAACGATTGCAAGAAATATCTCAACGAAAAAACATGCTCGGTTTGAATAATAGCGGAGCTGGATCTAGTGCGGCATCCCAAAGGCGAACAAGCTCATGCTTGCTTGATGGACCTGTGGTTGGAAGGTATGAAGACAAAAGGAAGATTATTGCTTTGTTGTCAAGAGATGAACCTAGTACTGTCAATTTCCATGTAGTTGCAATTGTTGGCATGCCTGGAGTTGGGAAGACTACACTTGCTAAATCAGTATTCAAGGACAACAATATTGCCCTGGAACAGTTCGATCTTAAGGTATGGGTATCAGTGTCTGATGATTTTGACCTTGTTAGAGTAACAAAAGCCATTCTTGAATCGGTCACGTCTGTGTATTGTGACTTCAAGGAATTCAATAAAGTTCAAGATAACTTGAGCAAGGAGTTAGCGAGTAAAAAGTTTCTAATTGTTTTGGATGATGTTTGGAATACCTGTGAACATGATCAATGGACAACCCTACAGTCTCCCTTTCATGTCGGAGCACCAGGAAGTAAGATAATTGTAACGACTCGTGATGAAAAAGTTGCAAGAATGACGAGACCCATTGAGATCCACTGTTTGAAGTATATGTCAGATGATGATTGTTGGGAAGTCTTTCAGCAGCATGCATTCATGAATGTCAACAGTGGGAGACCACAAGGTTTTGAATTTTTTCGAGAGAGTATTGTTAGAAAATGTGGCGGATTGCCCTTGGCTGCAAGGACTCTCGGTGGTCTTTTAGGTTGTGAAGACATAGATGAGTGGAAAAAAATATTGAACAATAGATTATGGAGTCTATCAAATAAGAGTCACATTCACCAAGTGTTGAAATTGAGCTATTACTATCTTCCTTCATATTTGAAAAGGTGCTTTGCATATTGCTCAATACTCCCAAACGACTATGAATTTGGGGAGAAGCAATTGACACTTCTGTGGATGGCAGAGGGTTTACTTCAACAACAAGAAGAACATGAAACATTAGAAGACTTGGGTGGTCACTATTTTGGACAGCTATTATCTAGGTCATTATTTCAAAAATCAAGCAGAAACAGTTCATTATATGTTATGCATCACCTTGTTGGTGATCTGGCTCGGTGGGCTGCGGGAGAAATTTGTCTCAGGTTGGAGGATAAAGATGGTAGATGTTCTCGAAAGACACGTCATTCGTCATACATTCCCGGTCAGTATGATGGGGTTAACAAATTTGAGGCATTCTCTGAAGCCAGACGTTTGCGTACTTTCCTACCATTGTCAGAAGACTTTTGTAACCATGTGACTTGTAAGGTTACTCTTGATTTACTGCCAAGATTGGAATACTTACGGGTACTCTCTTTGAATGGCTATCAAATAACTGAGTTGCCGAATTCAATTGGTAACTTGAAGCATCTACGGTACCTTGATGTTTCTCATACATTAATAATGAGATTGCCTGAATCAACAAGCACCCTTTGCAACTTACAGACATTAATATTAGAAAGTTGTTCTAGACTGAAGACACTGCCTACAAAGATGCGATATTTATCAAATCTACGCCATCTCAACAATTTAAATGTGCCTTCATTGGAAGGAATGCCCCCACAGCTAAGTCAACTGAGCAATCTGCAAACGTTGCCGAATTTCGTGGTGGGCAAAGGTAGTGACTCAAGGGTAAGAGAGATAGGGCCCCTGTCTCTTCTTCGAGGGACATTGTGCCTCTCAAGATTGGAAAATGTCAACGATGTTGAGGATGCAAGGAGGAGTGACTTAATTTGCAAGGAAGGGATTGATGCATTGCAGCTGGAATGGAATGATAAAGGACAAAATGAATCGATCGTCATCATCAAGGGTTATGGAGGTTTCCAGTTTTCAGCATGGATTGGAGATCCTTTATTCTCTTACGTGGTGCATGTGAGGTTAGAGAATTGTAACAATTGTCGATTCTTGCCACCACTTGGACAATTGCCTTCTCTCCAAATACTTTGTATTAGAAATATGTCTGGGGTGGAAAAAGTTGGCCTAGAGTTTTGTGGAGAGGGTAGCTTGTCTTTTCCATTATTGGAGACCCTTGTGTTTGAGGATATGCAAAACTGGAATGAGTGGTTTCCTTGCCAAGGATATCAAGGACTAGAAGTTTTCCCTTCCTTGAAAATACTTTCAGTGAAAAGATGTCCCAAATTAAAGGGTGAGTTACCTCACAAGCTTGATTTGTTAACAAAGCTTGAGATTCATGAATGTAATGAACTGGTGGTTTCAATTGGCAACTACAGACAGCTCTGTGAATTAAACGTGCGCGATTGCAAAGGGGTGGTGTATAAAAGTGTGCACCACTTTGAGTTACTCAAGTCTATTTATCTTTCAGATTTTTCAGTGCTGAGATTTCAAACTGGAGGATTCATGACCGGATTAAGAAAGGTGGAACAATTGATGATAGCCAATGGTGATGAACTTTCATCTGAATGGCAGAATGAAGGTAGATTGCTGCAACACCTGATATCTCTTCGAAGTTTGTTCATTGAAAGAAACTCCCACCTTCTTCAATTGCATTGCCTGACTTCACTTCAAATGCTTCACATATATAGATGCCCAAGTCTAGTTTCCATTCCAGAGTCATGTTTGCCAGCTTCTCTTAAAGATATAGAGATTGAAAGGTGCGGTTCTCTGACGTGCTTTGCAAGATACAAAATACCCCCGAGTCTAAGAACAATAGAGATTAAACATTGCCAGTGTTTGAAATATTTGGTAGAGGAGGAGCAGGTGGAGGGTTGCTCCTCTTCTTCATCTTCTCTTGAGTACTTGAGCATATCTAACTGTCCATCTCTTATCTCCTTATCATCCAGAGGTGGATTACCAAAGGCACTTAAACACCTTTACATACGGGATTGTGAACAACTGGAGACAATATCTGAAGGCTTCTATGATGACACTTGCCTCGAAGAACTTCATATTGGGCGTTGTGCAAATCTGAAATTCTTACCGGAGGGCCTATGGCTTCTTGCCAATCTTCGTAGGTTAATTGTTTTTGTCTGTGGAAGTCTTGTTTCCTTCCCAAGAGGAGGGTTGCCGACAAGGCCCTCCAACCTGACATTCCTTGTTATCTCCGATTGTCAAAGATGGAAAGCCTTCCCCAAAGGCATGATCAACCTTAACTCTCTCCAGGAGCTGGTGATTGGAAGCTGTGAAGGTTTGGCGTCCATTCTAGAGGAGGGTTTTCCCCCCAACCTAACATCACTTATCCTTTTGAATCCCAGCAATTGTAAGCCACTCACAGACTGGGGCTTGCACTTGCACAGACTCACCTCTCTTACAGAGTTATGCATCCAAGGTGTTGATCAAGACTTGGTTTCCTTTCCACCAGAGGAAATGCTGCTTCCTACATCTCTCATTAAACTTACCATTGGATTCTTCCCGAATCTGAGACGAATATCCAGCCAGGGCCTTCAAGTCCTCACCTCTCTTGAATCTCTTAGAATTGATGATTGCCCAAAGCTTGCATCCATTCCAGAGGACCTGCCCTCTTCACTCACACAACTTTACATCTATGATGACTGTCCTCTGCTTAGAAAGAGATATAAACCAGATATACCACGATACTGGGCCAAAATAAGTCACATTCCGTACATAGAGATTGGAGACTACAAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1337

Amino Acids

151.89

Weight (kDa)

6.62

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 98 4.2e-21 Rx N-terminal domain
NB-ARC PF00931 171 - 343 8.9e-37 NB-ARC domain
WHD_DRP PF23559 426 - 494 2.1e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 552 - 827 1.8e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 681 - 788 1.6e-18 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3112
Acc36I ACCTGC 2 cut(s) 3112, 3885
Acc65I GGTACC 1 cut(s) 1782
AccB1I GGYRCC 1 cut(s) 1782
AccB7I CCANNNNNTGG 2 cut(s) 2321, 3529
AccII CGCG 1 cut(s) 2661
AciI CCGC 6 cut(s) 102, 432, 449, 1092, 1493, 3021
AclI AACGTT 1 cut(s) 1997
AclWI GGATC 3 cut(s) 448, 955, 2246
AcsI RAATTY 9 cut(s) 77, 761, 1058, 1295, 1500, 1592, 1753, 2005, 3335
AcuI CTGAAG 4 cut(s) 20, 1629, 1901, 3294
AcyI GRCGYC 2 cut(s) 159, 3557
AdeI CACNNNGTG 1 cut(s) 1213
AfaI GTAC 7 cut(s) 315, 556, 1627, 1716, 1784, 3161, 3986
AfiI CCNNNNNNNGG 6 cut(s) 497, 599, 2321, 2480, 3422, 3529
AflII CTTAAG 1 cut(s) 665
AflIII ACRYGT 2 cut(s) 264, 1547
AjiI CACGTC 3 cut(s) 738, 1550, 3033
AjnI CCWGG 5 cut(s) 592, 648, 895, 3522, 3798
AjuI GAANNNNNNNTTGG 6 cut(s) 2947, 2979, 3297, 3329, 3437, 3469
AleI CACNNNNGTG 2 cut(s) 740, 1321
Alw21I GWGCWC 2 cut(s) 895, 2694
Alw26I GTCTC 9 cut(s) 879, 943, 1036, 1511, 2064, 2423, 3257, 3778, 3993
Alw44I GTGCAC 1 cut(s) 2690
AlwI GGATC 3 cut(s) 448, 955, 2246
AlwNI CAGNNNCTG 2 cut(s) 686, 3529
Ama87I CYCGRG 1 cut(s) 3058
AoxI GGCC 8 cut(s) 279, 319, 2051, 2383, 3349, 3437, 3802, 3963
ApaI GGGCCC 1 cut(s) 2055
ApaLI GTGCAC 1 cut(s) 2690
ApeKI GCWGC 7 cut(s) 26, 1012, 1106, 1490, 2164, 2848, 3733
ApoI RAATTY 9 cut(s) 77, 761, 1058, 1295, 1500, 1592, 1753, 2005, 3335
ArsI GACNNNNNNTTYG 6 cut(s) 1280, 1312, 1534, 1537, 1566, 1569
AseI ATTAAT 2 cut(s) 1805, 1856
Asp700I GAANNNNTTC 5 cut(s) 1005, 1062, 1441, 3306, 3488
Asp718I GGTACC 1 cut(s) 1782
AspLEI GCGC 1 cut(s) 2661
AspS9I GGNCC 8 cut(s) 488, 2051, 2052, 3349, 3438, 3802, 3874, 3963
AsuC2I CCSGG 1 cut(s) 1569
AsuHPI GGTGA 8 cut(s) 1199, 1454, 1484, 2564, 2819, 3544, 3655, 3808
AsuII TTCGAA 1 cut(s) 2869
AvaI CYCGRG 1 cut(s) 3058
AvaII GGWCC 2 cut(s) 488, 3874
BaeGI GKGCMC 2 cut(s) 2055, 2694
BanI GGYRCC 1 cut(s) 1782
BanII GRGCYC 1 cut(s) 2055
BarI GAAGNNNNNNTAC 2 cut(s) 737, 769
BauI CACGAG 2 cut(s) 63, 921
BbsI GAAGAC 6 cut(s) 516, 611, 1146, 1380, 1653, 1889
Bbv12I GWGCWC 2 cut(s) 895, 2694
BbvI GCAGC 7 cut(s) 13, 1024, 1093, 1477, 2176, 2835, 3720
BccI CCATC 7 cut(s) 479, 1321, 1520, 1574, 1932, 3190, 3474
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 5 cut(s) 594, 650, 897, 3524, 3800
BclI TGATCA 3 cut(s) 853, 3504, 3697
BcnI CCSGG 1 cut(s) 1569
BcoDI GTCTC 9 cut(s) 879, 943, 1036, 1511, 2064, 2423, 3257, 3778, 3993
BfaI CTAG 8 cut(s) 444, 552, 1410, 1875, 2387, 2495, 2960, 3566
BfmI CTRYAG 2 cut(s) 869, 2632
BfrI CTTAAG 1 cut(s) 665
BfuAI ACCTGC 2 cut(s) 3112, 3885
BglI GCCNNNNNGGC 1 cut(s) 3436
BisI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2849, 3734
BlsI GCNGC 8 cut(s) 28, 451, 1014, 1108, 1492, 2166, 2850, 3735
BmcAI AGTACT 2 cut(s) 556, 3161
Bme1390I CCNGG 6 cut(s) 594, 650, 897, 1569, 3524, 3800
Bme18I GGWCC 2 cut(s) 488, 3874
BmeT110I CYCGRG 1 cut(s) 3058
BmgBI CACGTC 3 cut(s) 738, 1550, 3033
BmgT120I GGNCC 8 cut(s) 488, 2051, 2052, 3349, 3438, 3802, 3874, 3963
BmiI GGNNCC 3 cut(s) 1784, 2053, 2054
BmrFI CCNGG 6 cut(s) 594, 650, 897, 1569, 3524, 3800
BmrI ACTGGG 2 cut(s) 3649, 3970
BmsI GCATC 9 cut(s) 461, 1468, 1783, 1890, 2107, 2146, 2939, 3693, 3869
BmuI ACTGGG 2 cut(s) 3649, 3970
BpiI GAAGAC 6 cut(s) 516, 611, 1146, 1380, 1653, 1889
BplI GAGNNNNNCTC 4 cut(s) 1589, 1621, 3119, 3151
BpmI CTGGAG 4 cut(s) 615, 2775, 3281, 3506
Bpu14I TTCGAA 1 cut(s) 2869
BpuEI CTTGAG 7 cut(s) 805, 2017, 2071, 2609, 2693, 3176, 3184
BpuMI CCSGG 1 cut(s) 1569
Bsa29I ATCGAT 1 cut(s) 2198
BsaAI YACGTR 1 cut(s) 2269
BsaBI GATNNNNATC 2 cut(s) 1909, 2204
BsaHI GRCGYC 2 cut(s) 159, 3557
BsaI GGTCTC 3 cut(s) 943, 1036, 2423
BsaJI CCNNGG 7 cut(s) 162, 282, 648, 1101, 2479, 3688, 3799
BsaWI WCCGGW 2 cut(s) 2768, 3343
Bsc4I CCNNNNNNNGG 6 cut(s) 497, 599, 2321, 2480, 3422, 3529
Bse1I ACTGG 8 cut(s) 2230, 2462, 2616, 2758, 3091, 3264, 3644, 3965
Bse3DI GCAATG 3 cut(s) 2159, 2911, 3085
Bse8I GATNNNNATC 2 cut(s) 1909, 2204
BseBI CCWGG 5 cut(s) 594, 650, 897, 3524, 3800
BseCI ATCGAT 1 cut(s) 2198
BseDI CCNNGG 7 cut(s) 162, 282, 648, 1101, 2479, 3688, 3799
BseGI GGATG 8 cut(s) 211, 452, 832, 1332, 2122, 3200, 3684, 3860
BseJI GATNNNNATC 2 cut(s) 1909, 2204
BseLI CCNNNNNNNGG 6 cut(s) 497, 599, 2321, 2480, 3422, 3529
BseMI GCAATG 3 cut(s) 2159, 2911, 3085
BseMII CTCAG 5 cut(s) 1522, 1734, 1973, 2732, 3773
BseNI ACTGG 8 cut(s) 2230, 2462, 2616, 2758, 3091, 3264, 3644, 3965
BseRI GAGGAG 7 cut(s) 179, 2140, 3127, 3128, 3131, 3435, 3584
BseSI GKGCMC 2 cut(s) 2055, 2694
BseXI GCAGC 7 cut(s) 13, 1024, 1093, 1477, 2176, 2835, 3720
BseYI CCCAGC 1 cut(s) 3614
Bsh1236I CGCG 1 cut(s) 2661
Bsh1285I CGRYCG 1 cut(s) 2202
BshFI GGCC 8 cut(s) 281, 321, 2053, 2385, 3351, 3439, 3804, 3965
BshNI GGYRCC 1 cut(s) 1782
BshVI ATCGAT 1 cut(s) 2198
BsiEI CGRYCG 1 cut(s) 2202
BsiHKAI GWGCWC 2 cut(s) 895, 2694
BsiHKCI CYCGRG 1 cut(s) 3058
BsiSI CCGG 3 cut(s) 1569, 2769, 3344
BslFI GGGAC 2 cut(s) 2086, 2523
BslI CCNNNNNNNGG 6 cut(s) 497, 599, 2321, 2480, 3422, 3529
BsmAI GTCTC 9 cut(s) 879, 943, 1036, 1511, 2064, 2423, 3257, 3778, 3993
BsmBI CGTCTC 1 cut(s) 3778
BsmFI GGGAC 2 cut(s) 2086, 2523
BsmI GAATGC 3 cut(s) 1019, 1601, 1965
BsnI GGCC 8 cut(s) 281, 321, 2053, 2385, 3351, 3439, 3804, 3965
Bso31I GGTCTC 3 cut(s) 943, 1036, 2423
BsoBI CYCGRG 1 cut(s) 3058
Bsp119I TTCGAA 1 cut(s) 2869
Bsp120I GGGCCC 1 cut(s) 2051
Bsp1286I GDGCHC 3 cut(s) 895, 2055, 2694
BspACI CCGC 6 cut(s) 102, 432, 449, 1092, 1493, 3021
BspANI GGCC 8 cut(s) 281, 321, 2053, 2385, 3351, 3439, 3804, 3965
BspCNI CTCAG 5 cut(s) 1521, 1735, 1974, 2733, 3774
BspDI ATCGAT 1 cut(s) 2198
BspFNI CGCG 1 cut(s) 2661
BspHI TCATGA 3 cut(s) 1023, 2596, 2763
BspLI GGNNCC 3 cut(s) 1784, 2053, 2054
BspMI ACCTGC 2 cut(s) 3112, 3885
BspPI GGATC 3 cut(s) 448, 955, 2246
BspT104I TTCGAA 1 cut(s) 2869
BspT107I GGYRCC 1 cut(s) 1782
BspTI CTTAAG 1 cut(s) 665
BspTNI GGTCTC 3 cut(s) 943, 1036, 2423
BsrDI GCAATG 3 cut(s) 2159, 2911, 3085
BsrI ACTGG 8 cut(s) 2230, 2462, 2616, 2758, 3091, 3264, 3644, 3965
BssECI CCNNGG 7 cut(s) 162, 282, 648, 1101, 2479, 3688, 3799
BssNI GRCGYC 2 cut(s) 159, 3557
BssSI CACGAG 2 cut(s) 63, 921
BssT1I CCWWGG 4 cut(s) 282, 1101, 2479, 3688
Bst2BI CACGAG 2 cut(s) 63, 921
Bst2UI CCWGG 5 cut(s) 594, 650, 897, 3524, 3800
Bst6I CTCTTC 4 cut(s) 2067, 2871, 3145, 3889
BstACI GRCGYC 2 cut(s) 159, 3557
BstAFI CTTAAG 1 cut(s) 665
BstAPI GCANNNNNTGC 2 cut(s) 1839, 1999
BstBAI YACGTR 1 cut(s) 2269
BstBI TTCGAA 1 cut(s) 2869
BstC8I GCNNGC 7 cut(s) 301, 479, 590, 1017, 2988, 3647, 3858
BstDEI CTNAG 9 cut(s) 1508, 1743, 1973, 1982, 2741, 3065, 3782, 3833, 3926
BstENI CCTNNNNNAGG 1 cut(s) 2478
BstF5I GGATG 8 cut(s) 211, 452, 832, 1332, 2122, 3200, 3684, 3860
BstFNI CGCG 1 cut(s) 2661
BstHHI GCGC 1 cut(s) 2661
BstMAI GTCTC 9 cut(s) 879, 943, 1036, 1511, 2064, 2423, 3257, 3778, 3993
BstMCI CGRYCG 1 cut(s) 2202
BstMWI GCNNNNNNNGC 6 cut(s) 143, 1487, 1839, 1969, 1999, 3436
BstNI CCWGG 5 cut(s) 594, 650, 897, 3524, 3800
BstNSI RCATGY 6 cut(s) 187, 268, 415, 592, 1019, 2279
BstSCI CCNGG 6 cut(s) 592, 648, 895, 1567, 3522, 3798
BstSFI CTRYAG 2 cut(s) 869, 2632
BstSLI GKGCMC 2 cut(s) 2055, 2694
BstUI CGCG 1 cut(s) 2661
BstV1I GCAGC 7 cut(s) 13, 1024, 1093, 1477, 2176, 2835, 3720
BstV2I GAAGAC 6 cut(s) 516, 611, 1146, 1380, 1653, 1889
BstX2I RGATCY 3 cut(s) 440, 960, 2251
BstXI CCANNNNNNTGG 1 cut(s) 3960
BstYI RGATCY 3 cut(s) 440, 960, 2251
Bsu15I ATCGAT 1 cut(s) 2198
BsuRI GGCC 8 cut(s) 281, 321, 2053, 2385, 3351, 3439, 3804, 3965
BsuTUI ATCGAT 1 cut(s) 2198
BtrI CACGTC 3 cut(s) 738, 1550, 3033
BtsCI GGATG 8 cut(s) 211, 452, 832, 1332, 2122, 3200, 3684, 3860
BtsI GCAGTG 3 cut(s) 12, 153, 1886
BveI ACCTGC 2 cut(s) 3112, 3885
Cac8I GCNNGC 7 cut(s) 301, 479, 590, 1017, 2988, 3647, 3858
CaiI CAGNNNCTG 2 cut(s) 686, 3529
CciI TCATGA 3 cut(s) 1023, 2596, 2763
CfoI GCGC 1 cut(s) 2661
Cfr13I GGNCC 8 cut(s) 488, 2051, 2052, 3349, 3438, 3802, 3874, 3963
ClaI ATCGAT 1 cut(s) 2198
CseI GACGC 2 cut(s) 167, 3546
Csp6I GTAC 7 cut(s) 314, 555, 1626, 1715, 1783, 3160, 3985
CviQI GTAC 7 cut(s) 314, 555, 1626, 1715, 1783, 3160, 3985
DdeI CTNAG 9 cut(s) 1508, 1743, 1973, 1982, 2741, 3065, 3782, 3833, 3926
DraI TTTAAA 1 cut(s) 1938
DraIII CACNNNGTG 1 cut(s) 1213
Eam1104I CTCTTC 4 cut(s) 2067, 2871, 3145, 3889
EarI CTCTTC 4 cut(s) 2067, 2871, 3145, 3889
EciI GGCGGA 1 cut(s) 1107
Eco130I CCWWGG 4 cut(s) 282, 1101, 2479, 3688
Eco147I AGGCCT 1 cut(s) 321
Eco24I GRGCYC 1 cut(s) 2055
Eco31I GGTCTC 3 cut(s) 943, 1036, 2423
Eco32I GATATC 2 cut(s) 2486, 2862
Eco47I GGWCC 2 cut(s) 488, 3874
Eco57I CTGAAG 4 cut(s) 20, 1629, 1901, 3294
Eco88I CYCGRG 1 cut(s) 3058
EcoNI CCTNNNNNAGG 1 cut(s) 2478
EcoO109I RGGNCCY 6 cut(s) 2051, 2052, 3349, 3438, 3802, 3874
EcoRI GAATTC 2 cut(s) 761, 1753
EcoRII CCWGG 5 cut(s) 592, 648, 895, 3522, 3798
EcoRV GATATC 2 cut(s) 2486, 2862
EcoT14I CCWWGG 4 cut(s) 282, 1101, 2479, 3688
EcoT22I ATGCAT 4 cut(s) 1021, 1461, 2161, 3686
EcoT38I GRGCYC 1 cut(s) 2055
ErhI CCWWGG 4 cut(s) 282, 1101, 2479, 3688
Esp3I CGTCTC 1 cut(s) 3778
FalI AAGNNNNNCTT 2 cut(s) 708, 740
FaqI GGGAC 2 cut(s) 2086, 2523
FauI CCCGC 2 cut(s) 95, 1486
FbaI TGATCA 3 cut(s) 853, 3504, 3697
Fnu4HI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2849, 3734
FokI GGATG 8 cut(s) 218, 439, 839, 1339, 2129, 3187, 3671, 3847
FriOI GRGCYC 1 cut(s) 2055
Fsp4HI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2849, 3734
FspBI CTAG 8 cut(s) 444, 552, 1410, 1875, 2387, 2495, 2960, 3566
GlaI GCGC 1 cut(s) 2660
GluI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2849, 3734
GsaI CCCAGC 1 cut(s) 3618
GsuI CTGGAG 4 cut(s) 615, 2775, 3281, 3506
HaeIII GGCC 8 cut(s) 281, 321, 2053, 2385, 3351, 3439, 3804, 3965
HapII CCGG 3 cut(s) 1569, 2769, 3344
HgaI GACGC 2 cut(s) 167, 3546
HhaI GCGC 1 cut(s) 2661
Hin1I GRCGYC 2 cut(s) 159, 3557
Hin6I GCGC 1 cut(s) 2659
HinP1I GCGC 1 cut(s) 2659
HincII GTYRAC 5 cut(s) 1033, 1588, 1979, 2104, 2580
HindII GTYRAC 5 cut(s) 1033, 1588, 1979, 2104, 2580
HindIII AAGCTT 4 cut(s) 107, 2567, 2585, 3854
HpaI GTTAAC 2 cut(s) 1588, 2580
HpaII CCGG 3 cut(s) 1569, 2769, 3344
HphI GGTGA 8 cut(s) 1199, 1454, 1484, 2564, 2819, 3544, 3655, 3808
Hpy166II GTNNAC 9 cut(s) 848, 1033, 1340, 1588, 1979, 2104, 2580, 2692, 3254
Hpy8I GTNNAC 9 cut(s) 848, 1033, 1340, 1588, 1979, 2104, 2580, 2692, 3254
HpyCH4IV ACGT 7 cut(s) 737, 1549, 1618, 1997, 2268, 2655, 3032
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 1487, 1839, 1969, 1999, 3436
HpyF3I CTNAG 9 cut(s) 1508, 1743, 1973, 1982, 2741, 3065, 3782, 3833, 3926
HpySE526I ACGT 7 cut(s) 737, 1549, 1618, 1997, 2268, 2655, 3032
Hsp92I GRCGYC 2 cut(s) 159, 3557
HspAI GCGC 1 cut(s) 2659
KpnI GGTACC 1 cut(s) 1786
Ksp22I TGATCA 3 cut(s) 853, 3504, 3697
KspAI GTTAAC 2 cut(s) 1588, 2580
LmnI GCTCC 6 cut(s) 286, 434, 890, 3118, 3140, 3526
Lsp1109I GCAGC 7 cut(s) 13, 1024, 1093, 1477, 2176, 2835, 3720
LweI GCATC 9 cut(s) 461, 1468, 1783, 1890, 2107, 2146, 2939, 3693, 3869
MaeI CTAG 8 cut(s) 444, 552, 1410, 1875, 2387, 2495, 2960, 3566
MaeII ACGT 7 cut(s) 737, 1549, 1618, 1997, 2268, 2655, 3032
MfeI CAATTG 9 cut(s) 579, 1310, 1758, 2299, 2327, 2622, 2789, 2906, 3619
MflI RGATCY 3 cut(s) 440, 960, 2251
MhlI GDGCHC 3 cut(s) 895, 2055, 2694
MlyI GAGTC 8 cut(s) 913, 1108, 1192, 1207, 2024, 2984, 3070, 3654
MmeI TCCRAC 4 cut(s) 478, 868, 1494, 3468
Mph1103I ATGCAT 4 cut(s) 1021, 1461, 2161, 3686
MroXI GAANNNNTTC 5 cut(s) 1005, 1062, 1441, 3306, 3488
MslI CAYNNNNRTG 6 cut(s) 182, 188, 263, 740, 1321, 2824
MspA1I CMGCKG 1 cut(s) 2167
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 3 cut(s) 1569, 2769, 3344
MspR9I CCNGG 6 cut(s) 594, 650, 897, 1569, 3524, 3800
MunI CAATTG 9 cut(s) 579, 1310, 1758, 2299, 2327, 2622, 2789, 2906, 3619
Mva1269I GAATGC 3 cut(s) 1019, 1601, 1965
MvaI CCWGG 5 cut(s) 594, 650, 897, 3524, 3800
MvnI CGCG 1 cut(s) 2661
MwoI GCNNNNNNNGC 6 cut(s) 143, 1487, 1839, 1969, 1999, 3436
NciI CCSGG 1 cut(s) 1569
NlaIV GGNNCC 3 cut(s) 1784, 2053, 2054
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 8 cut(s) 733, 749, 1199, 1385, 1663, 2027, 2129, 3974
NsiI ATGCAT 4 cut(s) 1021, 1461, 2161, 3686
NspI RCATGY 6 cut(s) 187, 268, 415, 592, 1019, 2279
NspV TTCGAA 1 cut(s) 2869
OliI CACNNNNGTG 2 cut(s) 740, 1321
PaeI GCATGC 2 cut(s) 592, 1019
PagI TCATGA 3 cut(s) 1023, 2596, 2763
PaqCI CACCTGC 1 cut(s) 3112
PceI AGGCCT 1 cut(s) 321
PciI ACATGT 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 1546
PctI GAATGC 3 cut(s) 1019, 1601, 1965
PdmI GAANNNNTTC 5 cut(s) 1005, 1062, 1441, 3306, 3488
PflMI CCANNNNNTGG 2 cut(s) 2321, 3529
PfoI TCCNGGA 1 cut(s) 3522
PkrI GCNGC 8 cut(s) 28, 451, 1014, 1108, 1492, 2166, 2850, 3735
Ple19I CGATCG 1 cut(s) 2202
PleI GAGTC 8 cut(s) 913, 1108, 1191, 1206, 2024, 2983, 3069, 3654
PpsI GAGTC 8 cut(s) 913, 1108, 1191, 1206, 2024, 2983, 3069, 3654
Ppu21I YACGTR 1 cut(s) 2269
PpuMI RGGWCCY 1 cut(s) 3874
PscI ACATGT 1 cut(s) 264
PshBI ATTAAT 2 cut(s) 1805, 1856
Psp1406I AACGTT 1 cut(s) 1997
Psp5II RGGWCCY 1 cut(s) 3874
Psp6I CCWGG 5 cut(s) 592, 648, 895, 3522, 3798
PspFI CCCAGC 1 cut(s) 3614
PspGI CCWGG 5 cut(s) 592, 648, 895, 3522, 3798
PspN4I GGNNCC 3 cut(s) 1784, 2053, 2054
PspOMI GGGCCC 1 cut(s) 2051
PspPI GGNCC 8 cut(s) 488, 2051, 2052, 3349, 3438, 3802, 3874, 3963
PspPPI RGGWCCY 1 cut(s) 3874
PstNI CAGNNNCTG 2 cut(s) 686, 3529
PsuI RGATCY 3 cut(s) 440, 960, 2251
PvuI CGATCG 1 cut(s) 2202
PvuII CAGCTG 1 cut(s) 2167
RsaI GTAC 7 cut(s) 315, 556, 1627, 1716, 1784, 3161, 3986
RsaNI GTAC 7 cut(s) 314, 555, 1626, 1715, 1783, 3160, 3985
RseI CAYNNNNRTG 6 cut(s) 182, 188, 263, 740, 1321, 2824
SatI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2849, 3734
Sau96I GGNCC 8 cut(s) 488, 2051, 2052, 3349, 3438, 3802, 3874, 3963
ScaI AGTACT 2 cut(s) 556, 3161
SchI GAGTC 8 cut(s) 913, 1108, 1192, 1207, 2024, 2984, 3070, 3654
ScrFI CCNGG 6 cut(s) 594, 650, 897, 1569, 3524, 3800
SduI GDGCHC 3 cut(s) 895, 2055, 2694
SfaNI GCATC 9 cut(s) 461, 1468, 1783, 1890, 2107, 2146, 2939, 3693, 3869
SfcI CTRYAG 2 cut(s) 869, 2632
SfuI TTCGAA 1 cut(s) 2869
SinI GGWCC 2 cut(s) 488, 3874
SmiI ATTTAAAT 1 cut(s) 1938
SmiMI CAYNNNNRTG 6 cut(s) 182, 188, 263, 740, 1321, 2824
SmlI CTYRAG 8 cut(s) 665, 784, 2032, 2086, 2588, 2708, 3155, 3163
SmoI CTYRAG 8 cut(s) 665, 784, 2032, 2086, 2588, 2708, 3155, 3163
SphI GCATGC 2 cut(s) 592, 1019
SseBI AGGCCT 1 cut(s) 321
SsiI CCGC 6 cut(s) 102, 432, 449, 1092, 1493, 3021
SspI AATATT 4 cut(s) 643, 1164, 1860, 3104
SspMI CTAG 8 cut(s) 444, 552, 1410, 1875, 2387, 2495, 2960, 3566
StuI AGGCCT 1 cut(s) 321
StyD4I CCNGG 6 cut(s) 592, 648, 895, 1567, 3522, 3798
StyI CCWWGG 4 cut(s) 282, 1101, 2479, 3688
SwaI ATTTAAAT 1 cut(s) 1938
TaiI ACGT 7 cut(s) 740, 1552, 1621, 2000, 2271, 2658, 3035
TaqII GACCGA 1 cut(s) 721
TatI WGTACW 2 cut(s) 554, 3159
TauI GCSGC 1 cut(s) 452
TseFI GTSAC 8 cut(s) 733, 749, 1199, 1385, 1663, 2027, 2129, 3974
TseI GCWGC 7 cut(s) 26, 1012, 1106, 1490, 2164, 2848, 3733
Tsp45I GTSAC 8 cut(s) 733, 749, 1199, 1385, 1663, 2027, 2129, 3974
TspGWI ACGGA 1 cut(s) 3972
Van91I CCANNNNNTGG 2 cut(s) 2321, 3529
Vha464I CTTAAG 1 cut(s) 665
VneI GTGCAC 1 cut(s) 2690
VpaK11BI GGWCC 2 cut(s) 488, 3874
VspI ATTAAT 2 cut(s) 1805, 1856
XagI CCTNNNNNAGG 1 cut(s) 2478
XapI RAATTY 9 cut(s) 77, 761, 1058, 1295, 1500, 1592, 1753, 2005, 3335
XbaI TCTAGA 2 cut(s) 1874, 3565
XceI RCATGY 6 cut(s) 187, 268, 415, 592, 1019, 2279
XcmI CCANNNNNNNNNTGG 1 cut(s) 3636
XmnI GAANNNNTTC 5 cut(s) 1005, 1062, 1441, 3306, 3488
XspI CTAG 8 cut(s) 444, 552, 1410, 1875, 2387, 2495, 2960, 3566
ZrmI AGTACT 2 cut(s) 556, 3161
Zsp2I ATGCAT 4 cut(s) 1021, 1461, 2161, 3686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.