RchiOBHm_Chr1g0319841

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
7464806 .. 7467791
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55005

Sequence Viewer

Length: 2946 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTGGGGAGATCCTTGGCTACTTTGGAAGCTTGGGAGGAGTTGGCAAAGAGCTGCAGAAATGGAGGGGAACACTGTCCGCAATTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAGCGAGGCAGTGGATCTGTGGCTGAATGACCTGAAACACTTGGCTTATGATCTGGACGATCTACTCAACACCTTTTCGAATGAGATGTTAGAACGCGAGCAGCTCAAGCTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTCCGAAGTAGACGAGATTGCTAATCGGTTGCAAGACATAGTTGACCGGAAAGAGAAACTCGGTTTGAAATATATTGAGCAAACCTCTACGTCAACATCGTCATCAAAAAGAACACCAAGTTCGCCTGTGTTGGATGGATCTGTGGTTGGGAGAGATGAAGACACAGGAAAGATTGTGGAATTGTTGTCCAGAAATTCTGATCCTTCTTCTCCCACAAATTATCAAGTTGTTGCCATTGTTGGTATGGGTGGACTCGGCAAGACAACGCTTGCGGGACAAGTCTTCAATGATGTAGTTGCAATGAAACAGTTTGATGATCTCAAGATCTGGGTTTCAGTATCCGATAACTTCGATCTTCAAACGGTGACGAGAGCTATTTTTAAGGAAGTTACATCTCGGCCTTGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTGAGCAAGGCGATAGATGGTAAAAGGTTTTTGATTGTTTTAGATGATGTCTGGAGAACATGTGATTATGATTCGTGGACAAAACTGCAAGCGCCCTTCCGTGGGGGAGCTAAGGGAAGTAAGATAATGGTGACAACACGTGACGAAAAAGTTGCAATATTGATGGGAGCCCCAGCCGGTGATGTTTATCATTTAAAGACTCTATCAGATGAAAGTTGTTTGCAAGTATTTGAGCAGCATGTTAGCAATGACAGGCCACCGAATTTTGAGTTGCTTAAGGAGAAAATTGTTACGAATTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTGAAGAAACTGGCAAGTGGGAGGAAATACTGAATGACAAATTATGGAGTATGTCAGATAGGAGTAAGATACTCCCAGTACTGAGATTGAGTTATCATTATCTCCCTACACCTTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTAATCCTTCTGTGGATGGCAGAGGGTTTCCTTGAGCAACCAGAAGGGACTAAAGCAATGGAAGATACCGGTGACGAATATTTTGGCGAGCTACTATCTCGGTCATTATTTCAAAAGGCGGGCAAAAACAGTTCATGCTACGTAATGCACGATCTTGTTGCTGATTTGGCACGATGGGCTGCTGGAGACACATTTTGCAGATTGGAGGATAAACTGCATGGTAGATGTTCTACAAATACTCGTCATCTAACTTACGTTTCTGGTAAGTTTGATGGGGTAAAAAGATTTGAGACACTTTCTGGAGCAAAACGCTTGCGGACTTTCCTACCACTTCTTGTTTCCGGTGGTTATGAGAATTATCTAACTCGTTATGCTACTTTTGATTTATTGCCACAATTGAAGTACTTGCGGGTGCTCTCCTTCAATGGCTATAAACTGACCGAGGTGCCAAATTCCGTAGGTAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGAGTTTGCCAGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAGTTGAAGACCCTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATGCCTTCATTCGAAGAAATGCCTCCCCAAGTAAGTCGGTTGACTCGTCTGCGAACTTTGCGTAATTTTGTGGTGGGGAAACGTAGTGCATCAAGGATTGGAGAGATAGGGTCATTGCATCTTCGTGGGACCTTGAGCGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGAATACCTTGCAATTGGAATGGAGTGGCACAAGTGAGAAGGAGTCGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTCTACATCTCACTATCAAAGGTTACAATGGTTTCGAATTTTCAAAATGGATTGGATATCCTTCATTCTCTGATATGACATTTGTGAAGTTGGAGAATTGTAAAAATTGTCAATTCTTACCTCCTCTCGGCCAATTACCATCTCTGAAAAACCTTTGGATAGAAGGACTGGCCAGTGTTGAAAGTGTGGGTGTTGAGTTTTACGGAGAGTGTAGCTTGCCTTTTCCAGTGTTGGAAGCCCTGATTTTCAAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCAAGTTTTGCCATGCATGGAAAAGCTTTTGGTCCTTAGTTGCCCCAAATTGGAAGGTTGGTTACCCAAGATCATGGATTCATTATCAGATCTGCGTATACGTGAATGTGAAGAGTTAGTGGTTTCAATAGCCAATTATAAACATCTTCGTAAGCTGTTCATCGACGGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGTCACTGGAGGATATGGGGCTTTATAATATTTCAGAGTTCAGACTCGAAATGGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAAATTTGTGGATTACTGATGGCTGCAACGTGGGATTTCTAATATTATTGGAGGCAACATGTCTGACTCCCCCTCTCACTTTATTCAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

981

Amino Acids

110.81

Weight (kDa)

6.44

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 1.1e-18 Rx N-terminal domain
NB-ARC PF00931 178 - 353 3.1e-38 NB-ARC domain
WHD_DRP PF23559 433 - 501 1e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 574 - 840 1.9e-24 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 691 - 809 3.8e-32 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2700, 2793
Acc65I GGTACC 1 cut(s) 1812
AccB1I GGYRCC 2 cut(s) 1777, 1812
AccI GTMKAC 3 cut(s) 381, 796, 2659
AccII CGCG 1 cut(s) 279
AciI CCGC 8 cut(s) 26, 138, 149, 164, 644, 1451, 1648, 1741
AclWI GGATC 4 cut(s) 64, 204, 517, 566
AcoI YGGCCR 2 cut(s) 2389, 2430
AcsI RAATTY 7 cut(s) 565, 1072, 1316, 1783, 2297, 2559, 2854
AcuI CTGAAG 1 cut(s) 20
AcvI CACGTG 1 cut(s) 950
AfaI GTAC 4 cut(s) 318, 1236, 1736, 1814
AfiI CCNNNNNNNGG 4 cut(s) 911, 912, 2387, 2611
AflII CTTAAG 1 cut(s) 1085
AflIII ACRYGT 3 cut(s) 869, 947, 2909
AgeI ACCGGT 1 cut(s) 1400
AjnI CCWGG 1 cut(s) 61
AjuI GAANNNNNNNTTGG 2 cut(s) 1323, 1355
AleI CACNNNNGTG 1 cut(s) 2751
Alw21I GWGCWC 2 cut(s) 1749, 2748
Alw26I GTCTC 3 cut(s) 1512, 1616, 1795
Alw44I GTGCAC 1 cut(s) 2744
AlwI GGATC 4 cut(s) 64, 204, 517, 566
AlwNI CAGNNNCTG 1 cut(s) 43
AoxI GGCC 4 cut(s) 770, 1064, 2389, 2430
ApaLI GTGCAC 1 cut(s) 2744
ApeKI GCWGC 9 cut(s) 43, 112, 146, 175, 283, 1045, 1127, 1511, 2874
ApoI RAATTY 7 cut(s) 565, 1072, 1316, 1783, 2297, 2559, 2854
ArsI GACNNNNNNTTYG 4 cut(s) 1301, 1333, 1397, 1429
AseI ATTAAT 2 cut(s) 1334, 1835
AsiGI ACCGGT 1 cut(s) 1400
Asp700I GAANNNNTTC 1 cut(s) 2559
Asp718I GGTACC 1 cut(s) 1812
AspLEI GCGC 1 cut(s) 904
AspS9I GGNCC 2 cut(s) 2100, 2593
AsuHPI GGTGA 5 cut(s) 287, 748, 952, 1001, 1415
AsuII TTCGAA 3 cut(s) 260, 1983, 2295
AvaII GGWCC 2 cut(s) 2100, 2593
BaeGI GKGCMC 1 cut(s) 2748
BalI TGGCCA 1 cut(s) 2432
BanI GGYRCC 2 cut(s) 1777, 1812
BanII GRGCYC 1 cut(s) 982
BarI GAAGNNNNNNTAC 2 cut(s) 2607, 2639
BbrPI CACGTG 1 cut(s) 950
BbsI GAAGAC 4 cut(s) 537, 646, 1919, 2156
Bbv12I GWGCWC 2 cut(s) 1749, 2748
BbvI GCAGC 9 cut(s) 30, 99, 158, 187, 295, 1057, 1114, 1498, 2861
BccI CCATC 8 cut(s) 500, 821, 967, 1342, 1500, 1598, 2407, 2864
BceAI ACGGC 1 cut(s) 2169
BcgI CGANNNNNNTGC 4 cut(s) 944, 978, 1472, 1506
BciT130I CCWGG 1 cut(s) 63
BciVI GTATCC 1 cut(s) 721
BcoDI GTCTC 3 cut(s) 1512, 1616, 1795
BfmI CTRYAG 1 cut(s) 113
BfoI RGCGCY 1 cut(s) 905
BfrI CTTAAG 1 cut(s) 1085
BfuI GTATCC 1 cut(s) 721
BglII AGATCT 3 cut(s) 15, 696, 2650
BmcAI AGTACT 2 cut(s) 1236, 1736
Bme1390I CCNGG 1 cut(s) 63
Bme18I GGWCC 2 cut(s) 2100, 2593
BmgT120I GGNCC 2 cut(s) 2100, 2593
BmiI GGNNCC 4 cut(s) 979, 1779, 1814, 2101
BmrFI CCNGG 1 cut(s) 63
BmrI ACTGGG 1 cut(s) 1226
BmsI GCATC 4 cut(s) 151, 2069, 2098, 2134
BmuI ACTGGG 1 cut(s) 1226
BpiI GAAGAC 4 cut(s) 537, 646, 1919, 2156
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 4 cut(s) 883, 1536, 1653, 2795
Bpu10I CCTNAGC 1 cut(s) 921
Bpu14I TTCGAA 3 cut(s) 260, 1983, 2295
BpuEI CTTGAG 4 cut(s) 272, 677, 1385, 2125
BsaAI YACGTR 3 cut(s) 950, 1474, 2663
BsaBI GATNNNNATC 1 cut(s) 996
BsaJI CCNNGG 4 cut(s) 62, 73, 910, 1773
BsaWI WCCGGW 3 cut(s) 417, 1400, 1673
BsaXI ACNNNNNCTCC 4 cut(s) 856, 886, 2219, 2249
Bsc4I CCNNNNNNNGG 4 cut(s) 911, 912, 2387, 2611
Bse118I RCCGGY 2 cut(s) 986, 1400
Bse1I ACTGG 7 cut(s) 307, 1171, 1232, 2433, 2433, 2486, 2778
Bse3DI GCAATG 5 cut(s) 678, 1063, 1117, 1395, 2084
Bse8I GATNNNNATC 1 cut(s) 996
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 4 cut(s) 62, 73, 910, 1773
BseGI GGATG 4 cut(s) 511, 790, 1353, 2149
BseJI GATNNNNATC 1 cut(s) 996
BseLI CCNNNNNNNGG 4 cut(s) 911, 912, 2387, 2611
BseMI GCAATG 5 cut(s) 678, 1063, 1117, 1395, 2084
BseMII CTCAG 2 cut(s) 803, 1229
BseNI ACTGG 7 cut(s) 307, 1171, 1232, 2433, 2433, 2486, 2778
BseRI GAGGAG 3 cut(s) 111, 182, 2373
BseSI GKGCMC 1 cut(s) 2748
BseXI GCAGC 9 cut(s) 30, 99, 158, 187, 295, 1057, 1114, 1498, 2861
BseYI CCCAGC 1 cut(s) 982
BsgI GTGCAG 1 cut(s) 2257
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 4 cut(s) 772, 1066, 2391, 2432
BshNI GGYRCC 2 cut(s) 1777, 1812
BshTI ACCGGT 1 cut(s) 1400
BsiHKAI GWGCWC 2 cut(s) 1749, 2748
BsiSI CCGG 4 cut(s) 418, 987, 1401, 1674
BslFI GGGAC 3 cut(s) 660, 1393, 2113
BslI CCNNNNNNNGG 4 cut(s) 911, 912, 2387, 2611
BsmAI GTCTC 3 cut(s) 1512, 1616, 1795
BsmFI GGGAC 3 cut(s) 660, 1393, 2113
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 4 cut(s) 772, 1066, 2391, 2432
Bsp119I TTCGAA 3 cut(s) 260, 1983, 2295
Bsp1286I GDGCHC 3 cut(s) 982, 1749, 2748
BspACI CCGC 8 cut(s) 26, 138, 149, 164, 644, 1451, 1648, 1741
BspANI GGCC 4 cut(s) 772, 1066, 2391, 2432
BspCNI CTCAG 2 cut(s) 804, 1230
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 4 cut(s) 979, 1779, 1814, 2101
BspMAI CTGCAG 1 cut(s) 117
BspPI GGATC 4 cut(s) 64, 204, 517, 566
BspT104I TTCGAA 3 cut(s) 260, 1983, 2295
BspT107I GGYRCC 2 cut(s) 1777, 1812
BspTI CTTAAG 1 cut(s) 1085
BsrDI GCAATG 5 cut(s) 678, 1063, 1117, 1395, 2084
BsrFI RCCGGY 2 cut(s) 986, 1400
BsrI ACTGG 7 cut(s) 307, 1171, 1232, 2433, 2433, 2486, 2778
BssAI RCCGGY 2 cut(s) 986, 1400
BssECI CCNNGG 4 cut(s) 62, 73, 910, 1773
BssNAI GTATAC 1 cut(s) 2660
BssT1I CCWWGG 1 cut(s) 73
Bst1107I GTATAC 1 cut(s) 2660
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 6 cut(s) 135, 681, 736, 1463, 1812, 2729
Bst6I CTCTTC 2 cut(s) 1268, 2667
BstAFI CTTAAG 1 cut(s) 1085
BstBAI YACGTR 3 cut(s) 950, 1474, 2663
BstBI TTCGAA 3 cut(s) 260, 1983, 2295
BstC8I GCNNGC 8 cut(s) 281, 642, 900, 1421, 1453, 1646, 1865, 2477
BstDEI CTNAG 4 cut(s) 812, 921, 1238, 2597
BstDSI CCRYGG 1 cut(s) 910
BstEII GGTNACC 1 cut(s) 2622
BstF5I GGATG 4 cut(s) 511, 790, 1353, 2149
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 1 cut(s) 905
BstHHI GCGC 1 cut(s) 904
BstMAI GTCTC 3 cut(s) 1512, 1616, 1795
BstMWI GCNNNNNNNGC 6 cut(s) 146, 289, 1499, 1508, 2029, 2153
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 6 cut(s) 873, 1052, 1863, 1867, 1975, 2913
BstPI GGTNACC 1 cut(s) 2622
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 113
BstSLI GKGCMC 1 cut(s) 2748
BstSNI TACGTA 1 cut(s) 1474
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 9 cut(s) 30, 99, 158, 187, 295, 1057, 1114, 1498, 2861
BstV2I GAAGAC 4 cut(s) 537, 646, 1919, 2156
BstX2I RGATCY 6 cut(s) 15, 69, 196, 509, 696, 2650
BstXI CCANNNNNNTGG 1 cut(s) 2635
BstYI RGATCY 6 cut(s) 15, 69, 196, 509, 696, 2650
BstZ17I GTATAC 1 cut(s) 2660
BsuI GTATCC 1 cut(s) 721
BsuRI GGCC 4 cut(s) 772, 1066, 2391, 2432
BtgI CCRYGG 1 cut(s) 910
BtsCI GGATG 4 cut(s) 511, 790, 1353, 2149
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 6 cut(s) 12, 131, 198, 2440, 2493, 2771
Cac8I GCNNGC 8 cut(s) 281, 642, 900, 1421, 1453, 1646, 1865, 2477
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 1 cut(s) 904
Cfr10I RCCGGY 2 cut(s) 986, 1400
Cfr13I GGNCC 2 cut(s) 2100, 2593
Csp6I GTAC 4 cut(s) 317, 1235, 1735, 1813
CspAI ACCGGT 1 cut(s) 1400
CspCI CAANNNNNGTGG 2 cut(s) 2840, 2875
CviQI GTAC 4 cut(s) 317, 1235, 1735, 1813
DdeI CTNAG 4 cut(s) 812, 921, 1238, 2597
DraI TTTAAA 1 cut(s) 1005
EaeI YGGCCR 2 cut(s) 2389, 2430
Eam1104I CTCTTC 2 cut(s) 1268, 2667
EarI CTCTTC 2 cut(s) 1268, 2667
Eco105I TACGTA 1 cut(s) 1474
Eco130I CCWWGG 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 982
Eco32I GATATC 1 cut(s) 2318
Eco47I GGWCC 2 cut(s) 2100, 2593
Eco57I CTGAAG 1 cut(s) 20
Eco72I CACGTG 1 cut(s) 950
Eco91I GGTNACC 1 cut(s) 2622
EcoO109I RGGNCCY 1 cut(s) 2100
EcoO65I GGTNACC 1 cut(s) 2622
EcoRII CCWGG 1 cut(s) 61
EcoRV GATATC 1 cut(s) 2318
EcoT14I CCWWGG 1 cut(s) 73
EcoT22I ATGCAT 2 cut(s) 1865, 2579
EcoT38I GRGCYC 1 cut(s) 982
ErhI CCWWGG 1 cut(s) 73
FalI AAGNNNNNCTT 2 cut(s) 1265, 1297
FaqI GGGAC 3 cut(s) 660, 1393, 2113
FauI CCCGC 3 cut(s) 637, 1444, 1734
FblI GTMKAC 3 cut(s) 381, 796, 2659
FokI GGATG 4 cut(s) 518, 797, 1360, 2156
FriOI GRGCYC 1 cut(s) 982
GlaI GCGC 1 cut(s) 903
GsaI CCCAGC 1 cut(s) 986
GsuI CTGGAG 4 cut(s) 883, 1536, 1653, 2795
HaeII RGCGCY 1 cut(s) 905
HaeIII GGCC 4 cut(s) 772, 1066, 2391, 2432
HapII CCGG 4 cut(s) 418, 987, 1401, 1674
HhaI GCGC 1 cut(s) 904
Hin6I GCGC 1 cut(s) 902
HinP1I GCGC 1 cut(s) 902
HincII GTYRAC 6 cut(s) 57, 415, 465, 797, 1857, 2013
HindII GTYRAC 6 cut(s) 57, 415, 465, 797, 1857, 2013
HindIII AAGCTT 5 cut(s) 88, 290, 2258, 2585, 2846
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 4 cut(s) 418, 987, 1401, 1674
HphI GGTGA 5 cut(s) 287, 748, 952, 1001, 1415
Hpy188III TCNNGA 7 cut(s) 236, 561, 694, 803, 862, 1632, 2938
Hpy99I CGWCG 1 cut(s) 2729
HpyCH4III ACNGT 6 cut(s) 135, 681, 736, 1463, 1812, 2729
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 289, 1499, 1508, 2029, 2153
HpyF3I CTNAG 4 cut(s) 812, 921, 1238, 2597
HspAI GCGC 1 cut(s) 902
KpnI GGTACC 1 cut(s) 1816
KspAI GTTAAC 1 cut(s) 57
LmnI GCTCC 3 cut(s) 917, 977, 1634
Lsp1109I GCAGC 9 cut(s) 30, 99, 158, 187, 295, 1057, 1114, 1498, 2861
LweI GCATC 4 cut(s) 151, 2069, 2098, 2134
MaeIII GTNAC 9 cut(s) 736, 760, 940, 950, 1099, 1403, 2282, 2622, 2769
MfeI CAATTG 3 cut(s) 141, 1727, 2192
MflI RGATCY 6 cut(s) 15, 69, 196, 509, 696, 2650
MhlI GDGCHC 3 cut(s) 982, 1749, 2748
MlsI TGGCCA 1 cut(s) 2432
MluNI TGGCCA 1 cut(s) 2432
MlyI GAGTC 8 cut(s) 618, 1003, 1862, 2008, 2231, 2777, 2805, 2911
MmeI TCCRAC 5 cut(s) 483, 2092, 2205, 2331, 2472
Mox20I TGGCCA 1 cut(s) 2432
Mph1103I ATGCAT 2 cut(s) 1865, 2579
MroXI GAANNNNTTC 1 cut(s) 2559
MscI TGGCCA 1 cut(s) 2432
MseI TTAA 9 cut(s) 56, 351, 753, 1004, 1086, 1334, 1835, 1886, 2760
MslI CAYNNNNRTG 2 cut(s) 2094, 2751
Msp20I TGGCCA 1 cut(s) 2432
MspA1I CMGCKG 2 cut(s) 149, 178
MspCI CTTAAG 1 cut(s) 1085
MspI CCGG 4 cut(s) 418, 987, 1401, 1674
MspR9I CCNGG 1 cut(s) 63
MunI CAATTG 3 cut(s) 141, 1727, 2192
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 1 cut(s) 63
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 6 cut(s) 146, 289, 1499, 1508, 2029, 2153
NlaIV GGNNCC 4 cut(s) 979, 1779, 1814, 2101
NmeAIII GCCGAG 3 cut(s) 606, 748, 2367
NmuCI GTSAC 5 cut(s) 736, 940, 950, 1403, 2769
NsiI ATGCAT 2 cut(s) 1865, 2579
NspI RCATGY 6 cut(s) 873, 1052, 1863, 1867, 1975, 2913
NspV TTCGAA 3 cut(s) 260, 1983, 2295
OliI CACNNNNGTG 1 cut(s) 2751
PaeI GCATGC 1 cut(s) 1867
PciI ACATGT 2 cut(s) 869, 2909
PcsI WCGNNNNNNNCGW 3 cut(s) 467, 1479, 2014
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 2559
PfeI GAWTC 2 cut(s) 881, 2639
PinAI ACCGGT 1 cut(s) 1400
PleI GAGTC 8 cut(s) 618, 1003, 1861, 2008, 2230, 2776, 2805, 2911
PmaCI CACGTG 1 cut(s) 950
PmlI CACGTG 1 cut(s) 950
PpsI GAGTC 8 cut(s) 618, 1003, 1861, 2008, 2230, 2776, 2805, 2911
Ppu21I YACGTR 3 cut(s) 950, 1474, 2663
PpuMI RGGWCCY 1 cut(s) 2100
PscI ACATGT 2 cut(s) 869, 2909
PshBI ATTAAT 2 cut(s) 1334, 1835
PsiI TTATAA 2 cut(s) 2700, 2793
Psp5II RGGWCCY 1 cut(s) 2100
Psp6I CCWGG 1 cut(s) 61
PspCI CACGTG 1 cut(s) 950
PspEI GGTNACC 1 cut(s) 2622
PspFI CCCAGC 1 cut(s) 982
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 4 cut(s) 979, 1779, 1814, 2101
PspPI GGNCC 2 cut(s) 2100, 2593
PspPPI RGGWCCY 1 cut(s) 2100
PstI CTGCAG 1 cut(s) 117
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 6 cut(s) 15, 69, 196, 509, 696, 2650
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 4 cut(s) 318, 1236, 1736, 1814
RsaNI GTAC 4 cut(s) 317, 1235, 1735, 1813
RseI CAYNNNNRTG 2 cut(s) 2094, 2751
SalI GTCGAC 1 cut(s) 795
SaqAI TTAA 9 cut(s) 56, 351, 753, 1004, 1086, 1334, 1835, 1886, 2760
Sau96I GGNCC 2 cut(s) 2100, 2593
ScaI AGTACT 2 cut(s) 1236, 1736
SchI GAGTC 8 cut(s) 618, 1003, 1862, 2008, 2231, 2777, 2805, 2911
ScrFI CCNGG 1 cut(s) 63
SduI GDGCHC 3 cut(s) 982, 1749, 2748
SfaNI GCATC 4 cut(s) 151, 2069, 2098, 2134
SfcI CTRYAG 1 cut(s) 113
SfuI TTCGAA 3 cut(s) 260, 1983, 2295
SinI GGWCC 2 cut(s) 2100, 2593
SmiMI CAYNNNNRTG 2 cut(s) 2094, 2751
SmlI CTYRAG 5 cut(s) 287, 692, 1085, 1364, 2104
SmoI CTYRAG 5 cut(s) 287, 692, 1085, 1364, 2104
SnaBI TACGTA 1 cut(s) 1474
SphI GCATGC 1 cut(s) 1867
SsiI CCGC 8 cut(s) 26, 138, 149, 164, 644, 1451, 1648, 1741
SspI AATATT 5 cut(s) 969, 1412, 1890, 2797, 2895
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 6 cut(s) 135, 681, 736, 1463, 1812, 2729
TaqI TCGA 7 cut(s) 260, 723, 796, 1983, 2295, 2724, 2814
TaqII GACCGA 2 cut(s) 1422, 1787
TatI WGTACW 2 cut(s) 1234, 1734
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 2 cut(s) 881, 2639
Tru1I TTAA 9 cut(s) 56, 351, 753, 1004, 1086, 1334, 1835, 1886, 2760
Tru9I TTAA 9 cut(s) 56, 351, 753, 1004, 1086, 1334, 1835, 1886, 2760
TscAI CASTG 6 cut(s) 12, 138, 198, 2440, 2493, 2778
TseFI GTSAC 5 cut(s) 736, 940, 950, 1403, 2769
TseI GCWGC 9 cut(s) 43, 112, 146, 175, 283, 1045, 1127, 1511, 2874
Tsp45I GTSAC 5 cut(s) 736, 940, 950, 1403, 2769
TspGWI ACGGA 3 cut(s) 899, 1777, 2478
TspRI CASTG 6 cut(s) 12, 138, 198, 2440, 2493, 2778
Vha464I CTTAAG 1 cut(s) 1085
VneI GTGCAC 1 cut(s) 2744
VpaK11BI GGWCC 2 cut(s) 2100, 2593
VspI ATTAAT 2 cut(s) 1334, 1835
XapI RAATTY 7 cut(s) 565, 1072, 1316, 1783, 2297, 2559, 2854
XceI RCATGY 6 cut(s) 873, 1052, 1863, 1867, 1975, 2913
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmiI GTMKAC 3 cut(s) 381, 796, 2659
XmnI GAANNNNTTC 1 cut(s) 2559
ZrmI AGTACT 2 cut(s) 1236, 1736
Zsp2I ATGCAT 2 cut(s) 1865, 2579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.