RchiOBHm_Chr1g0321351

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Forward (+)
8925184 .. 8926970
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55142

Sequence Viewer

Length: 1611 bp
ATGGTAGCGGAGGTCTTCCTCGGTGCATTCCTTCAGTTACTGCTCGACAGGTTAACTCCTCATCACGATTTGCTCAACTTGGCGCGCCTGCATGGCGTCGACAAGAAACTCAAGAAATGGAGTCCAGCGCTGTCTGCAATCGGAGCGGTGCTGCAGGACGCGGAGGACAAGCAACTGAAGAGCGAGGCTGTGAAGCTATGGCTGGATGAACTCAAACACTTGGCTTTTGACATGGATGACATCTTGGACACCTTTTCCACCGAGCTGTTGCGGCGCAACGTGATGAATCAGCAACATGCGTGGCTTACGAGTAAGGTACGGGGAGCTAAGTTCAATTTCAATATGAATTCGGAGATCAAGGAGATTAGTGATAGGTTGGTAGAGATATCTGAAAGAAAGGACTTGCTTGGGTTGAATTATGTTGTGAATAAATGTAGTCAGGTGTGGCAAAGGCCGCCGAGTTCATGTGTGTTAGATGGGCCTGTGGTTGGTAGGGATGATGATAAAAGGAAGATTGTTGAGGTGTTATTGAGGGATGATCAGCCTAGTAGTGTCAATTTCCATGTGGTTGCCATTGTGGGTATGCCAGGACTTGGAAAGACTACAATTGCAGGTCATGTCTTCAATGATGATGCAATGGAACGGTTTAGTCCCAAAGTATGGGTATCTGTGTCTGATAACTTCAATCTTGTGAGAGTGACAAAGGCAATTCTGGAATCAGTTACATCTGCACGCTGTGATTTGGAGGAGTTCAGTCAAATTCAGGAGATTCTGAGCAAGGCATTGGCAAGTAAAAAGTTTTTGGTTGTTTTAGATGATGTCTGGAACACATGTGACTATGATCTATGGGCAAAGCTGCAGTCTGCCTTTCGTGGTGGAGCATTGGGAAGTAAGGTAATTGTGACAACTCGTGACACACAAGTTGCTAAGATGATGAGATCCATTGAAGTTCATAACTTGGGGTGCATGTCAGATGATGATTGCTGGGAAGTCTTTGGGCAACATGCATTCTTGAATGTTGAGAATGGGAGACTGCAAAGCTTTGAGTTGTTTCGGGAGAAAGTTGTAGCAAAATGTGGTGGGTTGCCCTTGGCTGCAAGAGTCCTTGGTGGTCTTTTAGGCTGTAAAGAGATAGACGAATGGGAAGAAATATTGAACAGTAAATTATGGAGTCTATGTGATAACAGTGGCATTCTCCCAGTATTAAAATTGAGCTATCACTATCTCTCTTCAAATTTGAAAAGGTGCTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGAGGCAGTTGATCCTTCTATGGATGGCAGAGGGTCTCATTCAGCAGTCACATGAATGTAAACAACTGGAAGATTTAGGGAGTAACTATTTTCGGGAGCTATTGTCCAGGTCATTGTTTCAAAGGTCCAGTAAAAACCATTCACAATTTGTTATGCATGACCTTGTTGGTGATTTGTCACGGTGGGCTGCAGGAGACATGTGTTTCAGATTGGAGGATAAGCTGGATGTTAGATGTTCTTCAAAGGTTCGCCATTCCTCTTACATTCCTGGTCAATTTGATGGGGTTCAAAAATTCAAAGAATTTTTTGAAGTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

536

Amino Acids

60.91

Weight (kDa)

6.01

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 98 5.9e-21 Rx N-terminal domain
NB-ARC PF00931 166 - 337 4.1e-37 NB-ARC domain
WHD_DRP PF23559 422 - 489 1.3e-22 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 602
AccB7I CCANNNNNTGG 2 cut(s) 593, 660
AccBSI CCGCTC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 99
AccII CGCG 2 cut(s) 85, 161
AciI CCGC 5 cut(s) 8, 146, 161, 271, 455
AclWI GGATC 2 cut(s) 933, 1297
AcsI RAATTY 6 cut(s) 346, 759, 1234, 1283, 1583, 1592
AcuI CTGAAG 2 cut(s) 17, 197
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 737
AfaI GTAC 1 cut(s) 318
AfeI AGCGCT 1 cut(s) 129
AfiI CCNNNNNNNGG 3 cut(s) 488, 593, 660
AflIII ACRYGT 2 cut(s) 830, 1488
AjnI CCWGG 3 cut(s) 586, 1397, 1558
AluBI AGCT 8 cut(s) 196, 265, 326, 856, 1041, 1215, 1390, 1513
AluI AGCT 8 cut(s) 196, 265, 326, 856, 1041, 1215, 1390, 1513
Alw26I GTCTC 3 cut(s) 1024, 1331, 1479
AlwI GGATC 2 cut(s) 933, 1297
AlwNI CAGNNNCTG 1 cut(s) 40
Aor51HI AGCGCT 1 cut(s) 129
AoxI GGCC 2 cut(s) 452, 479
ApeKI GCWGC 4 cut(s) 151, 856, 1094, 1478
ApoI RAATTY 6 cut(s) 346, 759, 1234, 1283, 1583, 1592
ArsI GACNNNNNNTTYG 4 cut(s) 845, 877, 1268, 1300
AscI GGCGCGCC 1 cut(s) 83
AspLEI GCGC 4 cut(s) 85, 87, 130, 276
AspS9I GGNCC 2 cut(s) 479, 1416
AsuHPI GGTGA 1 cut(s) 1472
AvaII GGWCC 1 cut(s) 1416
BauI CACGAG 1 cut(s) 909
BbsI GAAGAC 2 cut(s) 7, 613
BbvI GCAGC 4 cut(s) 138, 843, 1081, 1465
BccI CCATC 3 cut(s) 470, 1309, 1565
BciT130I CCWGG 3 cut(s) 588, 1399, 1560
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 3 cut(s) 1024, 1331, 1479
BfaI CTAG 1 cut(s) 546
BfmI CTRYAG 3 cut(s) 152, 857, 1479
BfoI RGCGCY 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 602
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 6 cut(s) 152, 272, 455, 857, 1095, 1479
BlsI GCNGC 6 cut(s) 153, 273, 456, 858, 1096, 1480
Bme1390I CCNGG 3 cut(s) 588, 1399, 1560
Bme18I GGWCC 1 cut(s) 1416
BmgT120I GGNCC 2 cut(s) 479, 1416
BmrFI CCNGG 3 cut(s) 588, 1399, 1560
BmrI ACTGGG 1 cut(s) 1193
BmsI GCATC 1 cut(s) 622
BmuI ACTGGG 1 cut(s) 1193
BpiI GAAGAC 2 cut(s) 7, 613
BpuEI CTTGAG 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 1331
BsaJI CCNNGG 3 cut(s) 19, 1089, 1105
BsaXI ACNNNNNCTCC 6 cut(s) 1162, 1192, 1283, 1313, 1379, 1409
Bsc4I CCNNNNNNNGG 3 cut(s) 488, 593, 660
Bse1I ACTGG 3 cut(s) 1199, 1362, 1419
Bse3DI GCAATG 1 cut(s) 642
BseBI CCWGG 3 cut(s) 588, 1399, 1560
BseDI CCNNGG 3 cut(s) 19, 1089, 1105
BseGI GGATG 6 cut(s) 211, 241, 502, 541, 1320, 1522
BseLI CCNNNNNNNGG 3 cut(s) 488, 593, 660
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 1 cut(s) 764
BseNI ACTGG 3 cut(s) 1199, 1362, 1419
BsePI GCGCGC 1 cut(s) 83
BseRI GAGGAG 2 cut(s) 48, 761
BseXI GCAGC 4 cut(s) 138, 843, 1081, 1465
BseYI CCCAGC 1 cut(s) 984
BsgI GTGCAG 1 cut(s) 714
Bsh1236I CGCG 2 cut(s) 85, 161
BshFI GGCC 2 cut(s) 454, 481
BslFI GGGAC 1 cut(s) 636
BslI CCNNNNNNNGG 3 cut(s) 488, 593, 660
BsmAI GTCTC 3 cut(s) 1024, 1331, 1479
BsmFI GGGAC 1 cut(s) 636
BsmI GAATGC 3 cut(s) 26, 1007, 1191
BsnI GGCC 2 cut(s) 454, 481
Bso31I GGTCTC 1 cut(s) 1331
Bsp143I GATC 5 cut(s) 354, 538, 841, 938, 1302
BspACI CCGC 5 cut(s) 8, 146, 161, 271, 455
BspANI GGCC 2 cut(s) 454, 481
BspCNI CTCAG 1 cut(s) 765
BspFNI CGCG 2 cut(s) 85, 161
BspMAI CTGCAG 3 cut(s) 156, 861, 1483
BspMI ACCTGC 1 cut(s) 602
BspPI GGATC 2 cut(s) 933, 1297
BspQI GCTCTTC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 1331
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 642
BsrI ACTGG 3 cut(s) 1199, 1362, 1419
BssECI CCNNGG 3 cut(s) 19, 1089, 1105
BssHII GCGCGC 1 cut(s) 83
BssMI GATC 5 cut(s) 354, 538, 841, 938, 1302
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 909
BssT1I CCWWGG 2 cut(s) 1089, 1105
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 3 cut(s) 588, 1399, 1560
Bst4CI ACNGT 4 cut(s) 645, 1160, 1187, 1473
Bst6I CTCTTC 2 cut(s) 173, 1234
BstACI GRCGYC 1 cut(s) 96
BstC8I GCNNGC 3 cut(s) 85, 89, 733
BstDEI CTNAG 3 cut(s) 327, 773, 927
BstF5I GGATG 6 cut(s) 211, 241, 502, 541, 1320, 1522
BstFNI CGCG 2 cut(s) 85, 161
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 4 cut(s) 85, 87, 130, 276
BstKTI GATC 5 cut(s) 357, 541, 844, 941, 1305
BstMAI GTCTC 3 cut(s) 1024, 1331, 1479
BstMBI GATC 5 cut(s) 354, 538, 841, 938, 1302
BstMWI GCNNNNNNNGC 5 cut(s) 93, 134, 143, 271, 454
BstNI CCWGG 3 cut(s) 588, 1399, 1560
BstNSI RCATGY 5 cut(s) 299, 834, 970, 1007, 1492
BstSCI CCNGG 3 cut(s) 586, 1397, 1558
BstSFI CTRYAG 3 cut(s) 152, 857, 1479
BstUI CGCG 2 cut(s) 85, 161
BstV1I GCAGC 4 cut(s) 138, 843, 1081, 1465
BstV2I GAAGAC 2 cut(s) 7, 613
BstX2I RGATCY 1 cut(s) 938
BstYI RGATCY 1 cut(s) 938
BsuRI GGCC 2 cut(s) 454, 481
BtsCI GGATG 6 cut(s) 211, 241, 502, 541, 1320, 1522
BtsIMutI CAGTG 1 cut(s) 1192
BveI ACCTGC 1 cut(s) 602
Cac8I GCNNGC 3 cut(s) 85, 89, 733
CaiI CAGNNNCTG 1 cut(s) 40
CfoI GCGC 4 cut(s) 85, 87, 130, 276
Cfr13I GGNCC 2 cut(s) 479, 1416
CseI GACGC 2 cut(s) 85, 167
Csp6I GTAC 1 cut(s) 317
CspCI CAANNNNNGTGG 2 cut(s) 281, 316
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 3 cut(s) 327, 773, 927
DpnI GATC 5 cut(s) 356, 540, 843, 940, 1304
DpnII GATC 5 cut(s) 354, 538, 841, 938, 1302
DraIII CACNNNGTG 1 cut(s) 737
Eam1104I CTCTTC 2 cut(s) 173, 1234
EarI CTCTTC 2 cut(s) 173, 1234
Eco130I CCWWGG 2 cut(s) 1089, 1105
Eco31I GGTCTC 1 cut(s) 1331
Eco32I GATATC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 1416
Eco47III AGCGCT 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 17, 197
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 3 cut(s) 586, 1397, 1558
EcoRV GATATC 1 cut(s) 387
EcoT14I CCWWGG 2 cut(s) 1089, 1105
EcoT22I ATGCAT 2 cut(s) 1009, 1449
ErhI CCWWGG 2 cut(s) 1089, 1105
FaqI GGGAC 1 cut(s) 636
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 152, 272, 455, 857, 1095, 1479
FokI GGATG 6 cut(s) 218, 248, 509, 548, 1327, 1529
Fsp4HI GCNGC 6 cut(s) 152, 272, 455, 857, 1095, 1479
FspBI CTAG 1 cut(s) 546
GlaI GCGC 4 cut(s) 84, 86, 129, 275
GluI GCNGC 6 cut(s) 152, 272, 455, 857, 1095, 1479
GsaI CCCAGC 1 cut(s) 988
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 454, 481
HgaI GACGC 2 cut(s) 85, 167
HhaI GCGC 4 cut(s) 85, 87, 130, 276
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 4 cut(s) 83, 85, 128, 274
HinP1I GCGC 4 cut(s) 83, 85, 128, 274
HincII GTYRAC 2 cut(s) 54, 100
HindII GTYRAC 2 cut(s) 54, 100
HindIII AAGCTT 1 cut(s) 1039
HinfI GANTC 6 cut(s) 121, 286, 716, 769, 1101, 1171
HpaI GTTAAC 1 cut(s) 54
HphI GGTGA 1 cut(s) 1472
Hpy166II GTNNAC 3 cut(s) 54, 100, 1352
Hpy188I TCNGA 7 cut(s) 143, 352, 391, 676, 774, 973, 1499
Hpy188III TCNNGA 9 cut(s) 65, 112, 713, 764, 823, 911, 1012, 1055, 1385
Hpy8I GTNNAC 3 cut(s) 54, 100, 1352
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 41, 1316
HpyCH4III ACNGT 4 cut(s) 645, 1160, 1187, 1473
HpyCH4IV ACGT 1 cut(s) 279
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 134, 143, 271, 454
HpyF3I CTNAG 3 cut(s) 327, 773, 927
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 4 cut(s) 83, 85, 128, 274
Ksp22I TGATCA 1 cut(s) 538
KspAI GTTAAC 1 cut(s) 54
Kzo9I GATC 5 cut(s) 354, 538, 841, 938, 1302
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 4 cut(s) 143, 323, 878, 1387
Lsp1109I GCAGC 4 cut(s) 138, 843, 1081, 1465
LweI GCATC 1 cut(s) 622
MaeI CTAG 1 cut(s) 546
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 9 cut(s) 36, 697, 721, 833, 901, 911, 1338, 1373, 1467
MalI GATC 5 cut(s) 356, 540, 843, 940, 1304
MbiI CCGCTC 1 cut(s) 146
MboI GATC 5 cut(s) 354, 538, 841, 938, 1302
MboII GAAGA 8 cut(s) 7, 190, 523, 613, 1157, 1221, 1373, 1521
MfeI CAATTG 1 cut(s) 606
MflI RGATCY 1 cut(s) 938
MlyI GAGTC 3 cut(s) 130, 1110, 1180
Mph1103I ATGCAT 2 cut(s) 1009, 1449
MseI TTAA 2 cut(s) 53, 1205
MslI CAYNNNNRTG 1 cut(s) 1345
MspR9I CCNGG 3 cut(s) 588, 1399, 1560
MunI CAATTG 1 cut(s) 606
Mva1269I GAATGC 3 cut(s) 26, 1007, 1191
MvaI CCWGG 3 cut(s) 588, 1399, 1560
MvnI CGCG 2 cut(s) 85, 161
MwoI GCNNNNNNNGC 5 cut(s) 93, 134, 143, 271, 454
NdeII GATC 5 cut(s) 354, 538, 841, 938, 1302
NmeAIII GCCGAG 1 cut(s) 483
NmuCI GTSAC 6 cut(s) 697, 833, 901, 911, 1338, 1467
NsiI ATGCAT 2 cut(s) 1009, 1449
NspI RCATGY 5 cut(s) 299, 834, 970, 1007, 1492
PalAI GGCGCGCC 1 cut(s) 83
PauI GCGCGC 1 cut(s) 83
PciI ACATGT 2 cut(s) 830, 1488
PciSI GCTCTTC 1 cut(s) 173
PctI GAATGC 3 cut(s) 26, 1007, 1191
PfeI GAWTC 3 cut(s) 286, 716, 769
PflMI CCANNNNNTGG 2 cut(s) 593, 660
PkrI GCNGC 6 cut(s) 153, 273, 456, 858, 1096, 1480
PleI GAGTC 3 cut(s) 129, 1109, 1179
PpsI GAGTC 3 cut(s) 129, 1109, 1179
PscI ACATGT 2 cut(s) 830, 1488
Psp6I CCWGG 3 cut(s) 586, 1397, 1558
PspFI CCCAGC 1 cut(s) 984
PspGI CCWGG 3 cut(s) 586, 1397, 1558
PspPI GGNCC 2 cut(s) 479, 1416
PstI CTGCAG 3 cut(s) 156, 861, 1483
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 1 cut(s) 938
PteI GCGCGC 1 cut(s) 83
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 1345
SalI GTCGAC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 2 cut(s) 53, 1205
SatI GCNGC 6 cut(s) 152, 272, 455, 857, 1095, 1479
Sau3AI GATC 5 cut(s) 354, 538, 841, 938, 1302
Sau96I GGNCC 2 cut(s) 479, 1416
SchI GAGTC 3 cut(s) 130, 1110, 1180
ScrFI CCNGG 3 cut(s) 588, 1399, 1560
SfaNI GCATC 1 cut(s) 622
SfcI CTRYAG 3 cut(s) 152, 857, 1479
SgsI GGCGCGCC 1 cut(s) 83
SinI GGWCC 1 cut(s) 1416
SmiMI CAYNNNNRTG 1 cut(s) 1345
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SsiI CCGC 5 cut(s) 8, 146, 161, 271, 455
SspI AATATT 1 cut(s) 1152
SspMI CTAG 1 cut(s) 546
StyD4I CCNGG 3 cut(s) 586, 1397, 1558
StyI CCWWGG 2 cut(s) 1089, 1105
TaaI ACNGT 4 cut(s) 645, 1160, 1187, 1473
TaiI ACGT 1 cut(s) 282
TaqI TCGA 2 cut(s) 45, 99
TauI GCSGC 2 cut(s) 274, 457
TfiI GAWTC 3 cut(s) 286, 716, 769
Tru1I TTAA 2 cut(s) 53, 1205
Tru9I TTAA 2 cut(s) 53, 1205
TscAI CASTG 1 cut(s) 1192
TseFI GTSAC 6 cut(s) 697, 833, 901, 911, 1338, 1467
TseI GCWGC 4 cut(s) 151, 856, 1094, 1478
Tsp45I GTSAC 6 cut(s) 697, 833, 901, 911, 1338, 1467
TspDTI ATGAA 7 cut(s) 222, 299, 359, 453, 941, 1296, 1359
TspRI CASTG 1 cut(s) 1192
Van91I CCANNNNNTGG 2 cut(s) 593, 660
VpaK11BI GGWCC 1 cut(s) 1416
XapI RAATTY 6 cut(s) 346, 759, 1234, 1283, 1583, 1592
XceI RCATGY 5 cut(s) 299, 834, 970, 1007, 1492
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 1 cut(s) 546
Zsp2I ATGCAT 2 cut(s) 1009, 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.