Prupe.2G023600_v2.0.a1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000070
Physical Location & Seq
Forward (+)
2300807 .. 2309340
8534 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G023600.1

Sequence Viewer

Length: 4242 bp
ATGGCGGTGGGAGAGATTTTTCTTGGGGCGTTCCTTCAGTTGCTGCTCGACAGGTTGGCGCCTCGCGAGATCCTCAACTACTTTGGTCTCGTAAAGGGCGTCGACAAAAAGCTGAATAAATGGAGTGACAATTTGTCTGCAATTGTAGCGGTGCTGAATGATGCGGGGGAGAAGCAACTGACAGAGCATGGTGTGAAATTGTGGCTGGATGACCTCAGAGACTTGGCTTATGATGTAGAAGACGTGCTGGACAAATTTGCTACTAAAATATTGAAGCGCCAGATAGAGGGGCGTGATCAAGCCAGCACAAGTAAAAAGGTGCGGAGTTCATTCTCTAAACTGAAACTCAATTTCGATATGAACTCTGAAATAAAGAAGATTACAGAGCGGTTGCAAGAGATCTCTGAACGGAAAGATAAGTTTGGTTTAAAGGGCACCGGAACGTCTAGTAAAGCATGGTCAAGGCCACCAACTTCAGGTGTGCTTGGTGGACTAACCATTGTTGGAAGAGATGGAGATAAAGCAAAAATATTAGACATGTTGTCGAGAGATGAGCATAATAATGTCAACTTTCATGTTGTTGCCATTGTTGGCATGGCTGGCCTTGGGAAGACTACACTTGCTCAATTTGCATTCAACAACAACAGTGATGTCATGAAGGAGTTCGAGCCGAGGGTGTGGGTGTGTGTGTCCGATGACTTCGACATTGTAAGAGTGACGAAGGCAATTCTTGAATCAGTCACATCTCAACCCGTAAAAGTAGAGGAGTTCAGTAAAATGCAGCATGATTTGAATGAGCAGTTAAGAGGTAAAAAGTTTTTAATTGTTTTAGATGATATTTGGAACAAAGGTGATTTATATGATCTGTGGACAAGACTTCAGTCCCCTTTTAGCGTTGGAGCACAAGGAAGTAAGATAATTGTGACAACACGAGACCTAAAAGTTGCAAAGATTATGGGAGACACCGAAGTTCATAACTTGGAGTCTGTGTCAAATGATAATTGTTTGGAAATATTTGAGCAGCATGCATTTGTGAACAATGATAGACCACCAAATTTTGAGTTGCTTCGGAAAAAAATTGCTGCAAAATGCAGTGGATTGCCCTTAGCTGCAAGAACTCTTGGTGGGCTTTTACGTCAGAATGAAATAAACGAATGGGAAGAAATATTGAACAACAAATTGTGGAATTTATCGGGTAAGAGTGACATTCTTCCGGTATTAAAGTTGAGCTATCACTATCTTCCTTCCAACTTGAAGAGGTGTTTTGCCTATTGCTCAATATTTCCAAATGACTATGAATTTGGGGAGAAGCAATTGATCCTTTTATGGATGGCAGAGGGTTTGATTCAACAACCAGCAGAAGCCAATAGAAAAATGGAGGATTTAGGCCACGACTATTTTCAAGAGCTATTATGTAGGTCATTATTTCAAAAGGCAAGTGAAAACAATTCACGATATGTAATGCATGACCTTGTTACTGATTTGGCACAATGGGCAGCAGGAAATACTTGTTTTAGATTGGAGGACAAGAAAGGTGATAACTTGCAATCTGTATGCTTTCGTCATTCGTCTTTCATAATTGGTGACTATGATGGAGTTCAAAAGTTTGAGGCCTATCGCGAAGTTAAACGTTTGCGAACATTCCTGCCACTTTCACTATCAAATACTGGGTGGATTCGATATCGTTTGAGTGGACAAAATCTGGCTCGTACGGTAATTTTTGATTTATTACCTCAGATGCAATACTTACGGGTGCTTTCTTTGAATGGCTATCGAGTGACTGAGCTGCCAGATTCAATTGGTAATTTGAAGTATCTGCGGTATCTTGATTTTTCTCACACATGGATAACAAGTTTGCCTGAATCAACAACCACTCTTTTCAACTTACAGACACTGATATTGGAAGGTTGTAGTTTTTTGGAGGCACTACCCATAAACTTGAGGAATCTAGTGAATCTGCGTCATCTCAACAACTCATTTGCAAATGCATTGAAAGCAATGCCTCCGCAACTAGGTCGGTTGACAAATCTGCAATCTTTGCCTAATTTTGTGGTGGGTAAAGGAAGTGATGAATCAGGGATAAGAGAGATAGGATCCCTATCCCATCTCCGAGGGACATTATCGCTCTCAAGGTTGGAGAATGTGATCGATGCTGAGGATGCACGGAAGGCCGACTTAAAATCCAAGGAGAGAGTAGATGAATTGGTGCTAAAATGGTCGAGTGGCACACAAGAAACGCAATTGGGCGTGCTTGACAGATTAGAACCTCATAGAATGCTTGAAAAACTCATCATCAGGGGTTATGCTGGATTAGAATTTTCAACATGGATTGGAGATCGTTCGTTCTCCACTATGGTGCATGTACGCTTAGACGAATGTAAAAATTGTCAAATCTTGCCACCACTTGGGCAACTGCCTTTGCTCAAAGAACTTTATATTACAGGAATGGCTGCAGTGGAAATTGTTGGCCCTGAGTTTTATGGGGAGGGTAGCTTGCCTTTTCCAGTACTAGAGACTCTTGAGTTTGAGGATATGCAACACTGGAAGAAATGGGTTCCTTTTGTGGGGGATCGGGGAATTGGTGTTTTCCCTTGCTTGAAGTTTCTTTCAATCAGAAATTGTCCTCAATTGGAGGGTAAGGTGCCAGAGAACCTTGATTCTTTAGCAAGACTTACAATTATTAAATGCGAGGAATTGGTGATTTCAATTTCCAACTATAAACAAATTGGTGCATTAGACATCAACGGTTGCAAAGCGGTGGTGAAGACAAGTGGCGTTGAGTTTGAGTTATTAGAGTCCTTGCAACTTGCAAATATTTCAGAGGTGAAGCTCCAAACAGGGGAATTCACCAAAGGATTAAGGAAGGTTGCAAAATTGACGATTGGCGGATGTGAGGGGCTGACATCTTCACTGGAGAATGAGGATAGAGTATTGCAACACTTGATTTCTCTTGATTGTTTGGTTATTGAAGGAAACTCTTCCCTCCTTGAAAAGCTGGGAAAAGAAGCAGAGGAGTTGCTGCAATTGCAAATATTGACTTGCAAGCTTAAATATCTAGAATTAAACAAGTGTGCAAGTCTTTCGAAGGTACCAGAAGGGTTGCATCACCTAACGGCTCTTCAAGACCTTGAAATAGTTGGATGCTCAAGTCTGGTTTCTTTTCCAGATGTTGGTCTGCCTCCTTCCGTTGAAGTCATAAGGATTGAGGAGTGTGATTCGTTGCTGTATTTCGCAAAATACCAGATTCCCCCAAATCTAAGAAGAATAGAGATAAGGAGGTGTAAAAGTTTGAAATCATTAGTAGAGAAAGAGGAGGATAGTTCTTCGTCTTCCTCTTCTTCTCATATTTCTCTTGAGCACTTGGCAATACGGGATTGTGAATCTCTAAAGTCGTTATCATTGAGAGCCCAGTTGTTTCCCAAGGCGCTTAAACGCCTTCACATATCCCATTGTGGAGAGCTGCAGTTAATAATGTCCGATGAGTTAGCCCACGACAACACTAATTATTGTCTTGAATATATTAGCATCGATTCTTGCCCAAATCTGAAATCCTTACCGGAGGGCCTATGCCACCTCACCAATCTTAAAACTTTAGAGATTTATCGTTGTGGGAGTCTGGTTTCCATCCCGAGCCTGAGTGGTGAGGGTTTGCCTTCGCCAACAACAACAGCCGCCTCCAGCCTGAAACAAATCTACATTCAAAATTGCAACAAATTGGAAATGTTACCGGACATGGGCAATCTCAACTGTCTTCAGGGATTGAATATTGATTACAGTGAAGGTTTAAATTTCACTTCCTTTCCCCCCAACCTAACATCACTTACAATCCGCGGAATCAAGAATTGTAAGCCGCTGTGGGAGTTGTTGCACAGGCTCACATCTCTTACAGTGTTGCGGGTCGATGGTGAAGACCCATATGTTGTGTCGTTTCCACCCGAAGGTGACACGGGTATGGATACGGAGATGCTGCTCCCCGAATCTCTCACTCATCTCTTAATTCGGGGCTTCCCAAATCTGAAGAAACTGAGCAGCAAGGGCTTTCAATTCCTCACCTCTCTTCGATATCTTCTATTCTGCGATTGTCCAAAGCTGGCATCTATTCCAGTGGAGGGGTTGCCTATTTCACTGAGGGAACTTTATATCATTAAGTGTCCTCTGCTAAAAGATAAATGCCAGCCTGGAAGTAAAGGACGATACCTGCCCAAAATATCCCACATCCCCCGCATAAAGATAAGCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1414

Amino Acids

158.97

Weight (kDa)

6.39

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4206
Acc65I GGTACC 1 cut(s) 3088
AccB1I GGYRCC 4 cut(s) 58, 434, 2641, 3088
AccB7I CCANNNNNTGG 2 cut(s) 2405, 3170
AccBSI CCGCTC 1 cut(s) 388
AccI GTMKAC 1 cut(s) 102
AccII CGCG 3 cut(s) 66, 1620, 3831
AclI AACGTT 1 cut(s) 1630
AclWI GGATC 5 cut(s) 64, 1312, 2088, 2101, 2577
AcsI RAATTY 7 cut(s) 254, 1054, 1186, 1298, 2315, 2843, 3787
AcuI CTGAAG 5 cut(s) 20, 459, 863, 3737, 4037
AcyI GRCGYC 2 cut(s) 59, 99
AfaI GTAC 4 cut(s) 1711, 2364, 2508, 3090
AflIII ACRYGT 1 cut(s) 537
AhdI GACNNNNNGTC 2 cut(s) 133, 541
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 1 cut(s) 4177
AleI CACNNNNGTG 1 cut(s) 2354
Alw21I GWGCWC 2 cut(s) 904, 3360
Alw26I GTCTC 5 cut(s) 92, 213, 927, 954, 2507
AlwI GGATC 5 cut(s) 64, 1312, 2088, 2101, 2577
AlwNI CAGNNNCTG 2 cut(s) 43, 1894
Ama87I CYCGRG 1 cut(s) 3628
AoxI GGCC 7 cut(s) 464, 601, 1387, 1611, 2169, 2467, 3562
ApoI RAATTY 7 cut(s) 254, 1054, 1186, 1298, 2315, 2843, 3787
ArsI GACNNNNNNTTYG 2 cut(s) 1283, 1315
Asp700I GAANNNNTTC 2 cut(s) 662, 2977
Asp718I GGTACC 1 cut(s) 3088
AspLEI GCGC 3 cut(s) 61, 279, 3427
AspS9I GGNCC 2 cut(s) 2468, 3562
AsuII TTCGAA 1 cut(s) 3083
AvaI CYCGRG 1 cut(s) 3628
BaeGI GKGCMC 1 cut(s) 437
BamHI GGATCC 1 cut(s) 2093
BanI GGYRCC 4 cut(s) 58, 434, 2641, 3088
BanII GRGCYC 1 cut(s) 3409
BauI CACGAG 1 cut(s) 930
BbsI GAAGAC 6 cut(s) 246, 617, 2771, 3321, 3743, 3915
Bbv12I GWGCWC 2 cut(s) 904, 3360
BbvCI CCTCAGC 1 cut(s) 2154
BccI CCATC 6 cut(s) 506, 1324, 1586, 2112, 3632, 3896
BceAI ACGGC 1 cut(s) 3129
BcgI CGANNNNNNTGC 4 cut(s) 1997, 2031, 3063, 3097
BciT130I CCWGG 1 cut(s) 4179
BciVI GTATCC 1 cut(s) 3949
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 5 cut(s) 92, 213, 927, 954, 2507
BfaI CTAG 5 cut(s) 447, 1949, 2012, 2510, 3056
BfmI CTRYAG 2 cut(s) 2451, 3461
BfoI RGCGCY 3 cut(s) 62, 280, 3428
BfuAI ACCTGC 1 cut(s) 4206
BfuI GTATCC 1 cut(s) 3949
BglII AGATCT 1 cut(s) 399
BmcAI AGTACT 1 cut(s) 2508
Bme1390I CCNGG 1 cut(s) 4179
BmeRI GACNNNNNGTC 2 cut(s) 133, 541
BmeT110I CYCGRG 1 cut(s) 3628
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 2468, 3562
BmiI GGNNCC 6 cut(s) 60, 436, 2095, 2556, 2643, 3090
BmrFI CCNGG 1 cut(s) 4179
BmrI ACTGGG 2 cut(s) 1677, 3403
BmsI GCATC 9 cut(s) 151, 1728, 2140, 2149, 3112, 3131, 3534, 3954, 4103
BmuI ACTGGG 2 cut(s) 1677, 3403
BoxI GACNNNNGTC 1 cut(s) 880
BpiI GAAGAC 6 cut(s) 246, 617, 2771, 3321, 3743, 3915
BpmI CTGGAG 2 cut(s) 2933, 3661
Bpu10I CCTNAGC 2 cut(s) 1105, 2154
Bpu14I TTCGAA 1 cut(s) 3083
BpuEI CTTGAG 5 cut(s) 1960, 2113, 2540, 3130, 3374
Bsa29I ATCGAT 2 cut(s) 2148, 3528
BsaBI GATNNNNATC 1 cut(s) 2098
BsaHI GRCGYC 2 cut(s) 59, 99
BsaI GGTCTC 2 cut(s) 92, 927
BsaJI CCNNGG 6 cut(s) 604, 671, 2110, 2184, 3420, 3829
BsaWI WCCGGW 4 cut(s) 437, 1213, 3556, 3727
BsaXI ACNNNNNCTCC 2 cut(s) 974, 1004
Bse1I ACTGG 6 cut(s) 1672, 2504, 2546, 2916, 3409, 4103
Bse3DI GCAATG 1 cut(s) 2004
Bse8I GATNNNNATC 1 cut(s) 2098
BseBI CCWGG 1 cut(s) 4179
BseCI ATCGAT 2 cut(s) 2148, 3528
BseDI CCNNGG 6 cut(s) 604, 671, 2110, 2184, 3420, 3829
BseGI GGATG 7 cut(s) 214, 1335, 2164, 2894, 3146, 3624, 4215
BseJI GATNNNNATC 1 cut(s) 2098
BseMI GCAATG 1 cut(s) 2004
BseMII CTCAG 8 cut(s) 229, 1748, 1773, 2145, 2463, 3626, 4016, 4118
BseNI ACTGG 6 cut(s) 1672, 2504, 2546, 2916, 3409, 4103
BseRI GAGGAG 4 cut(s) 779, 3026, 3221, 3326
BseSI GKGCMC 1 cut(s) 437
BseYI CCCAGC 1 cut(s) 2995
Bsh1236I CGCG 3 cut(s) 66, 1620, 3831
BshFI GGCC 7 cut(s) 466, 603, 1389, 1613, 2171, 2469, 3564
BshNI GGYRCC 4 cut(s) 58, 434, 2641, 3088
BshVI ATCGAT 2 cut(s) 2148, 3528
BsiHKAI GWGCWC 2 cut(s) 904, 3360
BsiHKCI CYCGRG 1 cut(s) 3628
BsiSI CCGG 4 cut(s) 438, 1214, 3557, 3728
BsiWI CGTACG 1 cut(s) 1709
BslFI GGGAC 2 cut(s) 868, 2128
BsmAI GTCTC 5 cut(s) 92, 213, 927, 954, 2507
BsmFI GGGAC 2 cut(s) 868, 2128
BsmI GAATGC 2 cut(s) 632, 2280
BsnI GGCC 7 cut(s) 466, 603, 1389, 1613, 2171, 2469, 3564
Bso31I GGTCTC 2 cut(s) 92, 927
BsoBI CYCGRG 1 cut(s) 3628
Bsp119I TTCGAA 1 cut(s) 3083
Bsp1286I GDGCHC 4 cut(s) 437, 904, 3360, 3409
Bsp143I GATC 9 cut(s) 69, 295, 399, 862, 1317, 2093, 2145, 2335, 2569
Bsp68I TCGCGA 2 cut(s) 66, 1620
BspANI GGCC 7 cut(s) 466, 603, 1389, 1613, 2171, 2469, 3564
BspCNI CTCAG 8 cut(s) 228, 1747, 1774, 2146, 2464, 3627, 4017, 4119
BspDI ATCGAT 2 cut(s) 2148, 3528
BspFNI CGCG 3 cut(s) 66, 1620, 3831
BspHI TCATGA 1 cut(s) 654
BspLI GGNNCC 6 cut(s) 60, 436, 2095, 2556, 2643, 3090
BspMAI CTGCAG 2 cut(s) 2455, 3465
BspMI ACCTGC 1 cut(s) 4206
BspPI GGATC 5 cut(s) 64, 1312, 2088, 2101, 2577
BspQI GCTCTTC 1 cut(s) 3123
BspT104I TTCGAA 1 cut(s) 3083
BspT107I GGYRCC 4 cut(s) 58, 434, 2641, 3088
BspTNI GGTCTC 2 cut(s) 92, 927
BsrBI CCGCTC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 2004
BsrI ACTGG 6 cut(s) 1672, 2504, 2546, 2916, 3409, 4103
BssECI CCNNGG 6 cut(s) 604, 671, 2110, 2184, 3420, 3829
BssMI GATC 9 cut(s) 69, 295, 399, 862, 1317, 2093, 2145, 2335, 2569
BssNI GRCGYC 2 cut(s) 59, 99
BssSI CACGAG 1 cut(s) 930
BssT1I CCWWGG 3 cut(s) 604, 2184, 3420
Bst2BI CACGAG 1 cut(s) 930
Bst2UI CCWGG 1 cut(s) 4179
Bst4CI ACNGT 6 cut(s) 647, 1714, 2747, 3749, 3776, 3889
Bst6I CTCTTC 6 cut(s) 502, 1250, 2983, 3123, 3340, 4062
BstACI GRCGYC 2 cut(s) 59, 99
BstAPI GCANNNNNTGC 1 cut(s) 2038
BstBI TTCGAA 1 cut(s) 3083
BstC8I GCNNGC 8 cut(s) 304, 601, 1026, 2249, 2495, 3044, 4092, 4175
BstDSI CCRYGG 1 cut(s) 3829
BstF5I GGATG 7 cut(s) 214, 1335, 2164, 2894, 3146, 3624, 4215
BstFNI CGCG 3 cut(s) 66, 1620, 3831
BstH2I RGCGCY 3 cut(s) 62, 280, 3428
BstHHI GCGC 3 cut(s) 61, 279, 3427
BstKTI GATC 9 cut(s) 72, 298, 402, 865, 1320, 2096, 2148, 2338, 2572
BstMAI GTCTC 5 cut(s) 92, 213, 927, 954, 2507
BstMBI GATC 9 cut(s) 69, 295, 399, 862, 1317, 2093, 2145, 2335, 2569
BstNI CCWGG 1 cut(s) 4179
BstNSI RCATGY 3 cut(s) 541, 1028, 2363
BstPAI GACNNNNGTC 1 cut(s) 880
BstSCI CCNGG 1 cut(s) 4177
BstSFI CTRYAG 2 cut(s) 2451, 3461
BstSLI GKGCMC 1 cut(s) 437
BstUI CGCG 3 cut(s) 66, 1620, 3831
BstV2I GAAGAC 6 cut(s) 246, 617, 2771, 3321, 3743, 3915
BstX2I RGATCY 3 cut(s) 69, 399, 2093
BstYI RGATCY 3 cut(s) 69, 399, 2093
Bsu15I ATCGAT 2 cut(s) 2148, 3528
BsuI GTATCC 1 cut(s) 3949
BsuRI GGCC 7 cut(s) 466, 603, 1389, 1613, 2171, 2469, 3564
BsuTUI ATCGAT 2 cut(s) 2148, 3528
BtgI CCRYGG 1 cut(s) 3829
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 7 cut(s) 214, 1335, 2164, 2894, 3146, 3624, 4215
BtsI GCAGTG 2 cut(s) 1099, 2460
BtuMI TCGCGA 2 cut(s) 66, 1620
BveI ACCTGC 1 cut(s) 4206
Cac8I GCNNGC 8 cut(s) 304, 601, 1026, 2249, 2495, 3044, 4092, 4175
CaiI CAGNNNCTG 2 cut(s) 43, 1894
CciI TCATGA 1 cut(s) 654
CfoI GCGC 3 cut(s) 61, 279, 3427
Cfr13I GGNCC 2 cut(s) 2468, 3562
Cfr42I CCGCGG 1 cut(s) 3832
ClaI ATCGAT 2 cut(s) 2148, 3528
CseI GACGC 2 cut(s) 88, 1949
Csp6I GTAC 4 cut(s) 1710, 2363, 2507, 3089
CviQI GTAC 4 cut(s) 1710, 2363, 2507, 3089
DinI GGCGCC 1 cut(s) 60
DpnI GATC 9 cut(s) 71, 297, 401, 864, 1319, 2095, 2147, 2337, 2571
DpnII GATC 9 cut(s) 69, 295, 399, 862, 1317, 2093, 2145, 2335, 2569
DraI TTTAAA 2 cut(s) 429, 3786
DriI GACNNNNNGTC 2 cut(s) 133, 541
Eam1104I CTCTTC 6 cut(s) 502, 1250, 2983, 3123, 3340, 4062
Eam1105I GACNNNNNGTC 2 cut(s) 133, 541
EarI CTCTTC 6 cut(s) 502, 1250, 2983, 3123, 3340, 4062
EciI GGCGGA 1 cut(s) 2901
Eco130I CCWWGG 3 cut(s) 604, 2184, 3420
Eco147I AGGCCT 1 cut(s) 1613
Eco24I GRGCYC 1 cut(s) 3409
Eco31I GGTCTC 2 cut(s) 92, 927
Eco32I GATATC 2 cut(s) 1682, 4064
Eco57I CTGAAG 5 cut(s) 20, 459, 863, 3737, 4037
Eco88I CYCGRG 1 cut(s) 3628
EcoO109I RGGNCCY 1 cut(s) 3562
EcoRI GAATTC 1 cut(s) 2843
EcoRII CCWGG 1 cut(s) 4177
EcoRV GATATC 2 cut(s) 1682, 4064
EcoT14I CCWWGG 3 cut(s) 604, 2184, 3420
EcoT22I ATGCAT 3 cut(s) 1030, 1467, 1990
EcoT38I GRGCYC 1 cut(s) 3409
EgeI GGCGCC 1 cut(s) 60
EheI GGCGCC 1 cut(s) 60
ErhI CCWWGG 3 cut(s) 604, 2184, 3420
FalI AAGNNNNNCTT 2 cut(s) 2159, 2191
FaqI GGGAC 2 cut(s) 868, 2128
FauI CCCGC 3 cut(s) 157, 3888, 4229
FauNDI CATATG 1 cut(s) 3916
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 1 cut(s) 102
FokI GGATG 7 cut(s) 221, 1342, 2171, 2901, 3153, 3611, 4202
FriOI GRGCYC 1 cut(s) 3409
FspBI CTAG 5 cut(s) 447, 1949, 2012, 2510, 3056
GlaI GCGC 3 cut(s) 60, 278, 3426
GsaI CCCAGC 1 cut(s) 2999
GsuI CTGGAG 2 cut(s) 2933, 3661
HaeII RGCGCY 3 cut(s) 62, 280, 3428
HaeIII GGCC 7 cut(s) 466, 603, 1389, 1613, 2171, 2469, 3564
HapII CCGG 4 cut(s) 438, 1214, 3557, 3728
HgaI GACGC 2 cut(s) 88, 1949
HhaI GCGC 3 cut(s) 61, 279, 3427
Hin1I GRCGYC 2 cut(s) 59, 99
Hin6I GCGC 3 cut(s) 59, 277, 3425
HinP1I GCGC 3 cut(s) 59, 277, 3425
HincII GTYRAC 3 cut(s) 103, 568, 2022
HindII GTYRAC 3 cut(s) 103, 568, 2022
HindIII AAGCTT 1 cut(s) 3044
HpaII CCGG 4 cut(s) 438, 1214, 3557, 3728
Hpy166II GTNNAC 7 cut(s) 103, 491, 568, 870, 1036, 1694, 2022
Hpy8I GTNNAC 7 cut(s) 103, 491, 568, 870, 1036, 1694, 2022
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 6 cut(s) 647, 1714, 2747, 3749, 3776, 3889
HpyCH4IV ACGT 4 cut(s) 243, 443, 1135, 1630
HpySE526I ACGT 4 cut(s) 243, 443, 1135, 1630
Hsp92I GRCGYC 2 cut(s) 59, 99
HspAI GCGC 3 cut(s) 59, 277, 3425
KasI GGCGCC 1 cut(s) 58
KpnI GGTACC 1 cut(s) 3092
Ksp22I TGATCA 1 cut(s) 295
KspI CCGCGG 1 cut(s) 3832
Kzo9I GATC 9 cut(s) 69, 295, 399, 862, 1317, 2093, 2145, 2335, 2569
LguI GCTCTTC 1 cut(s) 3123
LmnI GCTCC 3 cut(s) 899, 2835, 3975
LweI GCATC 9 cut(s) 151, 1728, 2140, 2149, 3112, 3131, 3534, 3954, 4103
MaeI CTAG 5 cut(s) 447, 1949, 2012, 2510, 3056
MaeII ACGT 4 cut(s) 243, 443, 1135, 1630
MalI GATC 9 cut(s) 71, 297, 401, 864, 1319, 2095, 2147, 2337, 2571
MbiI CCGCTC 1 cut(s) 388
MboI GATC 9 cut(s) 69, 295, 399, 862, 1317, 2093, 2145, 2335, 2569
MfeI CAATTG 6 cut(s) 141, 1313, 1797, 2240, 2627, 3023
MflI RGATCY 3 cut(s) 69, 399, 2093
MhlI GDGCHC 4 cut(s) 437, 904, 3360, 3409
Mly113I GGCGCC 1 cut(s) 59
MlyI GAGTC 4 cut(s) 992, 2509, 2804, 3622
MmeI TCCRAC 6 cut(s) 484, 877, 1272, 2115, 2736, 3118
Mph1103I ATGCAT 3 cut(s) 1030, 1467, 1990
MroXI GAANNNNTTC 2 cut(s) 662, 2977
MslI CAYNNNNRTG 3 cut(s) 561, 2354, 3950
MspA1I CMGCKG 2 cut(s) 3831, 3853
MspI CCGG 4 cut(s) 438, 1214, 3557, 3728
MspR9I CCNGG 1 cut(s) 4179
MunI CAATTG 6 cut(s) 141, 1313, 1797, 2240, 2627, 3023
Mva1269I GAATGC 2 cut(s) 632, 2280
MvaI CCWGG 1 cut(s) 4179
MvnI CGCG 3 cut(s) 66, 1620, 3831
NarI GGCGCC 1 cut(s) 59
NdeI CATATG 1 cut(s) 3916
NdeII GATC 9 cut(s) 69, 295, 399, 862, 1317, 2093, 2145, 2335, 2569
NlaIV GGNNCC 6 cut(s) 60, 436, 2095, 2556, 2643, 3090
NmeAIII GCCGAG 1 cut(s) 696
NmuCI GTSAC 8 cut(s) 125, 715, 739, 922, 1202, 1583, 1777, 3941
NruI TCGCGA 2 cut(s) 66, 1620
NsiI ATGCAT 3 cut(s) 1030, 1467, 1990
NspI RCATGY 3 cut(s) 541, 1028, 2363
NspV TTCGAA 1 cut(s) 3083
OliI CACNNNNGTG 1 cut(s) 2354
PaeI GCATGC 1 cut(s) 1028
PagI TCATGA 1 cut(s) 654
PceI AGGCCT 1 cut(s) 1613
PciI ACATGT 1 cut(s) 537
PciSI GCTCTTC 1 cut(s) 3123
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 2 cut(s) 632, 2280
PdmI GAANNNNTTC 2 cut(s) 662, 2977
Pfl23II CGTACG 1 cut(s) 1709
PflMI CCANNNNNTGG 2 cut(s) 2405, 3170
PleI GAGTC 4 cut(s) 991, 2509, 2803, 3621
PluTI GGCGCC 1 cut(s) 62
PpsI GAGTC 4 cut(s) 991, 2509, 2803, 3621
PscI ACATGT 1 cut(s) 537
PshAI GACNNNNGTC 1 cut(s) 880
Psp1406I AACGTT 1 cut(s) 1630
Psp6I CCWGG 1 cut(s) 4177
PspFI CCCAGC 1 cut(s) 2995
PspGI CCWGG 1 cut(s) 4177
PspLI CGTACG 1 cut(s) 1709
PspN4I GGNNCC 6 cut(s) 60, 436, 2095, 2556, 2643, 3090
PspPI GGNCC 2 cut(s) 2468, 3562
PstI CTGCAG 2 cut(s) 2455, 3465
PstNI CAGNNNCTG 2 cut(s) 43, 1894
PsuI RGATCY 3 cut(s) 69, 399, 2093
RruI TCGCGA 2 cut(s) 66, 1620
RsaI GTAC 4 cut(s) 1711, 2364, 2508, 3090
RsaNI GTAC 4 cut(s) 1710, 2363, 2507, 3089
RseI CAYNNNNRTG 3 cut(s) 561, 2354, 3950
SacII CCGCGG 1 cut(s) 3832
SalI GTCGAC 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 3123
Sau3AI GATC 9 cut(s) 69, 295, 399, 862, 1317, 2093, 2145, 2335, 2569
Sau96I GGNCC 2 cut(s) 2468, 3562
ScaI AGTACT 1 cut(s) 2508
SchI GAGTC 4 cut(s) 992, 2509, 2804, 3622
ScrFI CCNGG 1 cut(s) 4179
SduI GDGCHC 4 cut(s) 437, 904, 3360, 3409
SfaNI GCATC 9 cut(s) 151, 1728, 2140, 2149, 3112, 3131, 3534, 3954, 4103
SfcI CTRYAG 2 cut(s) 2451, 3461
SfoI GGCGCC 1 cut(s) 60
Sfr303I CCGCGG 1 cut(s) 3832
SfuI TTCGAA 1 cut(s) 3083
SgrBI CCGCGG 1 cut(s) 3832
SmiMI CAYNNNNRTG 3 cut(s) 561, 2354, 3950
SmlI CTYRAG 5 cut(s) 1939, 2128, 2519, 3145, 3353
SmoI CTYRAG 5 cut(s) 1939, 2128, 2519, 3145, 3353
SphI GCATGC 1 cut(s) 1028
SseBI AGGCCT 1 cut(s) 1613
SspDI GGCGCC 1 cut(s) 58
SspI AATATT 8 cut(s) 270, 531, 1014, 1167, 1281, 2815, 3033, 3766
SspMI CTAG 5 cut(s) 447, 1949, 2012, 2510, 3056
StuI AGGCCT 1 cut(s) 1613
StyD4I CCNGG 1 cut(s) 4177
StyI CCWWGG 3 cut(s) 604, 2184, 3420
TaaI ACNGT 6 cut(s) 647, 1714, 2747, 3749, 3776, 3889
TaiI ACGT 4 cut(s) 246, 446, 1138, 1633
TatI WGTACW 1 cut(s) 2506
TauI GCSGC 2 cut(s) 3674, 3853
TseFI GTSAC 8 cut(s) 125, 715, 739, 922, 1202, 1583, 1777, 3941
Tsp45I GTSAC 8 cut(s) 125, 715, 739, 922, 1202, 1583, 1777, 3941
TspGWI ACGGA 4 cut(s) 424, 2179, 3175, 3974
Van91I CCANNNNNTGG 2 cut(s) 2405, 3170
XapI RAATTY 7 cut(s) 254, 1054, 1186, 1298, 2315, 2843, 3787
XbaI TCTAGA 1 cut(s) 3055
XceI RCATGY 3 cut(s) 541, 1028, 2363
XcmI CCANNNNNNNNNTGG 2 cut(s) 592, 1372
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 2 cut(s) 662, 2977
XspI CTAG 5 cut(s) 447, 1949, 2012, 2510, 3056
ZrmI AGTACT 1 cut(s) 2508
Zsp2I ATGCAT 3 cut(s) 1030, 1467, 1990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.